EWM20706.1, cds9967 (CDS) Nannochloropsis gaditana | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

Overview


Name	EWM20706.1
Unique Name	cds9967
Type	CDS
Organism	Nannochloropsis gaditana (N. gaditana B-31)
Alignment location	KK037259.1:92900..93082 -
Alignment location	KK037259.1:92523..92705 -

Link to JBrowse

Properties


Property Name	Value
Protein id	EWM20706.1
Product	trna -lysidine synthetase
Orig transcript id	gnl\|cribi\|Naga_102325g1.10078.mrna
Gbkey	CDS

Mutants

Expression

No biomaterial libraries express this feature.

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
KK037259.1	supercontig	KK037259.1:92900..93082 -
KK037259.1	supercontig	KK037259.1:92523..92705 -

Analyses

This CDS is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST analysis for N. gaditana B-31	2020-04-07
InterPro analysis for N. gaditana B-31	2020-04-06
Gene prediction for N. gaditana B-31	2014-02-18

Annotated Terms

The following terms have been associated with this CDS:

Vocabulary: **INTERPRO**
Term	Definition
IPR014729	Rossmann-like_a/b/a_fold
IPR011063	tRNA-lysidine/thiocyt_synth

Homology

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|585100540|gb|EWM20706.1| (trna -lysidine synthetase [Nannochloropsis gaditana])

HSP 1 Score: 248.825 bits (634), Expect = 1.068e-83
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0

Query:    1 MQEHDIHVITRVYPANLTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFKVRGKG 121
            MQEHDIHVITRVYPANLTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFKVRGKG
Sbjct:    1 MQEHDIHVITRVYPANLTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFKVRGKG 121

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|1214663798|gb|OWY92577.1| (TRNA(Ile)-lysidine synthetase, partial [Phytophthora megakarya])

HSP 1 Score: 115.931 bits (289), Expect = 2.092e-28
Identity = 52/100 (52.00%), Postives = 69/100 (69.00%), Query Frame = 0

Query:   17 LTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFK 116
            + R T  R+ YE  +REIRYSTY E + Q     +  GHHRGDV+ENV+SN+ +G+  L+L+GM  +  VNGV + RPLLD  K  I+DFAH +G+PYFK
Sbjct:   39 VKRATTRRDDYEKISREIRYSTYAEVMEQYNIPGMCFGHHRGDVQENVISNMMKGLSLLNLNGMQASSIVNGVRIWRPLLDFDKDVIFDFAHRYGIPYFK 138

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|1210513041|dbj|GAX27959.1| (hypothetical protein FisN_32Lh019 [Fistulifera solaris])

HSP 1 Score: 117.857 bits (294), Expect = 2.380e-28
Identity = 54/100 (54.00%), Postives = 68/100 (68.00%), Query Frame = 0

Query:   17 LTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFK 116
            +TR    R+ YE  AREIRY  YR+ + +     +LLGHH+GD+ ENVLSN  +G GPL LSGM      +GV++ RPLL L KS I+D+AH FGVPYFK
Sbjct:  345 VTRGITARDEYERIAREIRYQAYRDAIAEFDGIGILLGHHKGDLRENVLSNAHKGCGPLDLSGMTAVSQNDGVTLLRPLLPLEKSTIFDYAHQFGVPYFK 444

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|325187233|emb|CCA21772.1| (tRNA(Ile)lysidine synthase putative [Albugo laibachii Nc14])

HSP 1 Score: 117.087 bits (292), Expect = 4.301e-28
Identity = 52/100 (52.00%), Postives = 70/100 (70.00%), Query Frame = 0

Query:   17 LTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFK 116
            +TR    R+ YE  AR+IRYSTYR  L +     +  GHHRGDV+ENV+SN+ +G+  L L+GM+ T T NGV + RP+L+  KS I+DFAH +G+PYFK
Sbjct:  292 VTRGETKRDEYEKIARDIRYSTYRSILEKHSIPGICFGHHRGDVQENVVSNMMKGLSLLSLNGMSETSTANGVVIWRPMLEFDKSTIFDFAHRYGIPYFK 391

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|1210520479|dbj|GAX20637.1| (hypothetical protein FisN_32Hh019 [Fistulifera solaris])

HSP 1 Score: 115.546 bits (288), Expect = 1.737e-27
Identity = 53/100 (53.00%), Postives = 67/100 (67.00%), Query Frame = 0

Query:   17 LTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFK 116
            +TR    R+ YE  AREIRY  YR+ + +     +LLGHH+GD+ ENVLSN  +G GPL LSGM      +GV++ RPLL L K  I+D+AH FGVPYFK
Sbjct:  379 VTRGITARDEYERIAREIRYQAYRDAIEEFDGIGILLGHHKGDLRENVLSNAHKGCGPLDLSGMTAVSQNDGVTLLRPLLPLEKIAIFDYAHQFGVPYFK 478

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|612388973|ref|XP_007510007.1| (tRNA(Ile)-lysidine synthase [Bathycoccus prasinos] >gi|412985676|emb|CCO19122.1| tRNA(Ile)-lysidine synthase [Bathycoccus prasinos])

HSP 1 Score: 115.161 bits (287), Expect = 2.543e-27
Identity = 55/115 (47.83%), Postives = 76/115 (66.09%), Query Frame = 0

Query:    2 QEHDIHV-ITRVYPANLTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYF 115
            ++HDI   I  +    L R    RE YE+EAR+IR+  Y+    + +  AVLLGHH GDV+ENV++N+FRG   L+++GM+  G +  V + RP+L  PKS + DFAHTFGVPYF
Sbjct:  390 RKHDIACEIKNMNNEGLKRGVTPRETYEIEARKIRFDFYKLMRQKYEYPAVLLGHHDGDVQENVITNMFRGANVLNVNGMSDEGIIENVRIWRPMLSRPKSDVLDFAHTFGVPYF 504

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|673039283|ref|XP_008873602.1| (tRNA(Ile)-lysidine synthetase, variant [Aphanomyces invadans] >gi|574471032|gb|ETV98041.1| tRNA(Ile)-lysidine synthetase, variant [Aphanomyces invadans])

HSP 1 Score: 114.775 bits (286), Expect = 2.702e-27
Identity = 55/114 (48.25%), Postives = 74/114 (64.91%), Query Frame = 0

Query:    3 EHDIHVITRVYPANLTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFK 116
            EH+I    R   +   R T  RE YE  +R+IRY+TY+E + +     + +GHHRGDV+ENV+SN+ +G   L L+GM  +  VNGV + RPLL + K  I+DFAH FGVPYFK
Sbjct:  281 EHNIECFVRPI-SEFQRATTKREDYENLSRDIRYTTYQEVMAKHSIPGMCVGHHRGDVQENVISNMMKGQSLLGLNGMTPSSVVNGVRIWRPLLSVTKDAIFDFAHVFGVPYFK 393

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|673039281|ref|XP_008873601.1| (tRNA(Ile)-lysidine synthetase [Aphanomyces invadans] >gi|574471031|gb|ETV98040.1| tRNA(Ile)-lysidine synthetase [Aphanomyces invadans])

HSP 1 Score: 114.39 bits (285), Expect = 3.908e-27
Identity = 55/114 (48.25%), Postives = 74/114 (64.91%), Query Frame = 0

Query:    3 EHDIHVITRVYPANLTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFK 116
            EH+I    R   +   R T  RE YE  +R+IRY+TY+E + +     + +GHHRGDV+ENV+SN+ +G   L L+GM  +  VNGV + RPLL + K  I+DFAH FGVPYFK
Sbjct:  281 EHNIECFVRPI-SEFQRATTKREDYENLSRDIRYTTYQEVMAKHSIPGMCVGHHRGDVQENVISNMMKGQSLLGLNGMTPSSVVNGVRIWRPLLSVTKDAIFDFAHVFGVPYFK 393

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|397571890|gb|EJK48013.1| (hypothetical protein THAOC_33226 [Thalassiosira oceanica])

HSP 1 Score: 114.39 bits (285), Expect = 4.508e-27
Identity = 56/107 (52.34%), Postives = 69/107 (64.49%), Query Frame = 0

Query:   17 LTRNTAVRERYEVEAREIRYSTYRETLFQTQASA-------VLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFK 116
            +TR    R+ YE  AREIR+  YR T  +  +         ++LGHHRGD+ ENVLSN  RG GPL LSGM    T +GV + RPLL L KS IYD++HT+GVPYFK
Sbjct:  547 ITRGVTARDDYERIAREIRFDIYRRTCAEVSSDGTVQRGVGIMLGHHRGDLRENVLSNAHRGCGPLDLSGMTEVSTNDGVVLFRPLLFLEKSFIYDYSHTYGVPYFK 653

[Close]

BLAST of EWM20706.1 vs. NCBI_GenBank
Match: gi|301106300|ref|XP_002902233.1| (tRNA(Ile)-lysidine synthase, putative [Phytophthora infestans T30-4] >gi|262098853|gb|EEY56905.1| tRNA(Ile)-lysidine synthase, putative [Phytophthora infestans T30-4])

HSP 1 Score: 114.005 bits (284), Expect = 5.512e-27
Identity = 52/100 (52.00%), Postives = 70/100 (70.00%), Query Frame = 0

Query:   17 LTRNTAVRERYEVEAREIRYSTYRETLFQTQASAVLLGHHRGDVEENVLSNVFRGVGPLHLSGMAVTGTVNGVSVHRPLLDLPKSQIYDFAHTFGVPYFK 116
            + R T  R+ YE  +REIRYSTY E + +     +  GHHRGDV+ENV+SN+ +G+  L+L+GM  +  VNGV + RPLLDL K  I++FAH +GVPYFK
Sbjct:  295 VKRATTRRDDYEKISREIRYSTYAEVMQKYHIPGMCFGHHRGDVQENVISNMMKGLSLLNLNGMQASSVVNGVRIWRPLLDLEKDVIFEFAHRYGVPYFK 394

The following BLAST results are available for this feature:

BLAST of EWM20706.1 vs. NCBI_GenBank

BLAST of EWM20706.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10


Match Name	E-value	Identity	Description
gi\|585100540\|gb\|EWM20706.1\|	1.068e-83	100.00	trna -lysidine synthetase [Nannochloropsis gaditan...	[more]
gi\|1214663798\|gb\|OWY92577.1\|	2.092e-28	52.00	TRNA(Ile)-lysidine synthetase, partial [Phytophtho...	[more]
gi\|1210513041\|dbj\|GAX27959.1\|	2.380e-28	54.00	hypothetical protein FisN_32Lh019 [Fistulifera sol...	[more]
gi\|325187233\|emb\|CCA21772.1\|	4.301e-28	52.00	tRNA(Ile)lysidine synthase putative [Albugo laibac...	[more]
gi\|1210520479\|dbj\|GAX20637.1\|	1.737e-27	53.00	hypothetical protein FisN_32Hh019 [Fistulifera sol...	[more]
gi\|612388973\|ref\|XP_007510007.1\|	2.543e-27	47.83	tRNA(Ile)-lysidine synthase [Bathycoccus prasinos]...	[more]
gi\|673039283\|ref\|XP_008873602.1\|	2.702e-27	48.25	tRNA(Ile)-lysidine synthetase, variant [Aphanomyce...	[more]
gi\|673039281\|ref\|XP_008873601.1\|	3.908e-27	48.25	tRNA(Ile)-lysidine synthetase [Aphanomyces invadan...	[more]
gi\|397571890\|gb\|EJK48013.1\|	4.508e-27	52.34	hypothetical protein THAOC_33226 [Thalassiosira oc...	[more]
gi\|301106300\|ref\|XP_002902233.1\|	5.512e-27	52.00	tRNA(Ile)-lysidine synthase, putative [Phytophthor...	[more]

Relationships

This CDS is a part of the following mRNA feature(s):


Feature Name	Unique Name	Species	Type
rna9998	rna9998	Nannochloropsis gaditana (N. gaditana B-31)	mRNA

Sequences

Synonyms

Publications