EWM21269.1, cds9403 (CDS) Nannochloropsis gaditana

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|585101423|gb|EWM21269.1| (glycoprotein 3-alpha-l-fucosyltransferase a [Nannochloropsis gaditana])

HSP 1 Score: 784.252 bits (2024), Expect = 0.000e+0
Identity = 376/376 (100.00%), Postives = 376/376 (100.00%), Query Frame = 0

Query:    1 MRAQENPCRLFLCVPQAPLPAVLVVVKTMPCIFSGVLAAFTRSPPHCRPSPPLPASSFLFPHPSNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQPDIHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFLTEPFTVATLHATLLALYGSEHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            MRAQENPCRLFLCVPQAPLPAVLVVVKTMPCIFSGVLAAFTRSPPHCRPSPPLPASSFLFPHPSNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQPDIHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFLTEPFTVATLHATLLALYGSEHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV
Sbjct:    1 MRAQENPCRLFLCVPQAPLPAVLVVVKTMPCIFSGVLAAFTRSPPHCRPSPPLPASSFLFPHPSNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQPDIHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFLTEPFTVATLHATLLALYGSEHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376          

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|545360017|ref|XP_005645422.1| (hypothetical protein COCSUDRAFT_37671 [Coccomyxa subellipsoidea C-169] >gi|384247391|gb|EIE20878.1| hypothetical protein COCSUDRAFT_37671 [Coccomyxa subellipsoidea C-169])

HSP 1 Score: 238.424 bits (607), Expect = 3.877e-71
Identity = 137/329 (41.64%), Postives = 189/329 (57.45%), Query Frame = 0

Query:   51 PPLPASSFLFPHPSNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQPDIH-KDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFLTEP-FTVATLHATLLALYG-SEHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            P +P  + +    SNC A SFRL+A+      N TVHSYGRCL+N      K D L   KF+LAFENS+E+DY+TEKY QAL  G+VP+VIGA NI DY  + NS++ +E++  +P VAA+MQ L+ +  AYD MLAWK  GP D+FLAL+D+  V   C LC+ LA R+R  E       A++   + PC+CR    G       V+HHL VRER  FE R +FL     TVA LHA +   +G +   P+W   RP ++  + +       + ++I++VY  G T + ALYG+         +   D  +   V  NPC ++EV FV
Sbjct:  133 PLVPKEADVVAFISNCGAKSFRLQALEALSA-NFTVHSYGRCLHNVDTRDPKADVLRRYKFTLAFENSQELDYITEKYIQALEAGSVPIVIGATNIADYEVAPNSMLVLESMEDIPAVAARMQQLLRNKTAYDEMLAWKEIGPQDKFLALLDLSVVHSSCSLCIQLATRIRTAE-----EAAQQLQRQPPCSCRRAPGG------AVVHHLLVRERGRFESRDVFLDGGNLTVAGLHAAINQAFGRAGQVPIWQSHRPDFKGGAREIGKFNTLIKLRIYRVYQVGQTQRAALYGE--------ASLDTDLKVAGIVSGNPCAKLEVVFV 441          

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|719969937|ref|XP_010269886.1| (PREDICTED: glycoprotein 3-alpha-L-fucosyltransferase A-like isoform X1 [Nelumbo nucifera])

HSP 1 Score: 234.958 bits (598), Expect = 4.986e-69
Identity = 131/317 (41.32%), Postives = 186/317 (58.68%), Query Frame = 0

Query:   64 SNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQP-DIHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGY-RRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFL-TEPFTVATLHATLLALY-GSEHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            SNC +++FRLEA+   +K N+ + SYG C  N+   + K + L   KFSLAFENS E DYVTEK+FQ+L  GA+PVV+GAPNI ++APS  S++HI+ L+ +P +A  M+ L  +  AY+  L WK  GPSD F ALVD+  V   CRLC+YLA ++R  E        +  G+ +RPC C    E         I+H+YVRER  FE  S+FL +   T+  L + +LA +  S H P+W ++RP   K             +KI+++YP G+T ++ALY      F   G    D  LR++V+ +PC + EV FV
Sbjct:  230 SNCRSHNFRLEALEGLEKENIKIDSYGICHRNRDGSVDKVETLKRYKFSLAFENSNEEDYVTEKFFQSLVAGAIPVVVGAPNIQEFAPSTGSVLHIKELSDIPSIAKAMKYLAENPDAYNQSLRWKYEGPSDSFKALVDMAAVHSSCRLCIYLASKIRESE-------ERNPGFQKRPCKCTRGLE--------TIYHIYVRERGRFEMDSIFLRSVNLTLNALESAVLAKFKSSNHVPIWKKQRPESIKGD----------ELKIYRIYPVGMTQRQALY-----TFKFNG----DSDLRKYVESHPCPKFEVIFV 512          

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|302754078|ref|XP_002960463.1| (fucosyltransferase [Selaginella moellendorffii] >gi|300171402|gb|EFJ38002.1| fucosyltransferase [Selaginella moellendorffii])

HSP 1 Score: 233.802 bits (595), Expect = 9.854e-69
Identity = 134/319 (42.01%), Postives = 186/319 (58.31%), Query Frame = 0

Query:   64 SNCHANSFRLEAISFFQKHNVTVHSYGRCLNN-QPDIHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRV---RRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFL-TEPFTVATLHATLLALYGSE-HRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            SNC  ++FRLEAI+  Q + V V SYG CL N  P + K D L H KFSLAFENS E DYVTEK++Q+L  G+VPVVIGAPNI ++APS NSI++++++  +  VA QM+ L  +  AY+  L WK  GP+D F AL+D+  V   CRLC+++A  +   +  EAF+ R  +      RPC C         GS     HLYVRER  F   S+FL ++  +VA L A +++ + S+ H P+W   RP   K         A  P+K+H+VYP G+T +EAL+            +  D+ L   +  NPC ++EV FV
Sbjct:  212 SNCGGHNFRLEAITKLQGYGVAVDSYGSCLTNVHPAVEKVDALRHYKFSLAFENSNEEDYVTEKFWQSLEAGSVPVVIGAPNIQNFAPSPNSILYLKSIKDVQAVAKQMKYLARNATAYNETLRWKFEGPTDSFKALIDMAAVHSSCRLCIHVATAIHQKQEQEAFRSRNAS------RPCKCSV-------GSTTTF-HLYVRERGRFHLESIFLRSDNLSVAALEAAIISKFSSKNHVPIWNAARPEMLK---------AKGPLKVHRVYPVGLTQREALFSW----------QFTDEDLVNHIDANPCAKLEVIFV 497          

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|641840787|gb|KDO59704.1| (hypothetical protein CISIN_1g038682mg, partial [Citrus sinensis])

HSP 1 Score: 230.335 bits (586), Expect = 1.033e-68
Identity = 135/331 (40.79%), Postives = 188/331 (56.80%), Query Frame = 0

Query:   49 PSPPLPASSFLFPHPSNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQPD-IHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFL-TEPFTVATLHATLLALYGS-EHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            P  P   S+      SNC A +FRL+A+   +K NV + SYG C  N+   + K + L   KFSLAFENS E DYVTEK+FQAL  G+VPVV+GAPNI D+APS  SI+HI     +  VA  M+ L  +  AY+  L+WK  GPSD F ALVD+  V   CRLC+YLA ++      ++R +      +RPC C    E         +HHLYVRER  F+  S+FL +   T+  L + +LA + S +H P+W ++RP    KS  G  ++     KI++++P G+T ++ALY      F   G    D  L+R ++ +PC + EV FV
Sbjct:   84 PVQPKTESALAAAFISNCGARNFRLQALEALEKENVKIDSYGGCHRNRDGRVEKVETLKRYKFSLAFENSNEEDYVTEKFFQALVAGSVPVVVGAPNIQDFAPSPKSILHIREREDVKSVAKTMKYLAENPEAYNQSLSWKYEGPSDSFKALVDMAAVHSSCRLCIYLATKI------QEREETNPAFRKRPCKCTRGSE--------TVHHLYVRERGRFKMVSIFLRSGSLTLKALESAVLAKFKSLKHVPIWKQERP----KSIRGEDEL-----KIYRIHPVGLTQRQALY-----EFKFNG----DDDLKRHIESSPCAKFEVIFV 382          

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|659112246|ref|XP_008456133.1| (PREDICTED: glycoprotein 3-alpha-L-fucosyltransferase A-like [Cucumis melo])

HSP 1 Score: 233.417 bits (594), Expect = 1.834e-68
Identity = 132/321 (41.12%), Postives = 185/321 (57.63%), Query Frame = 0

Query:   64 SNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQ--PDIHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRV----RRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFL-TEPFTVATLHATLLALYGS-EHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            SNC A +FRLEA+   +K N+T+ SYG C  N+    ++K + L   KFSLAFENS E DYVTEK+FQ+L  GA+PVVIGAPNI D+APS  S++HI  L+ +  VA  M+ L A+  A++  L WK  GPSD F AL D+G V   CRLC+++A  +     +G AFK+          RPC C    E         +HHLYVRER  F   S+FL +   TV  L + +++ + S +H P+W ++RP   K   +         ++++K+YP G+T ++ALY      F   G    D   RR+V+ NPC ++E  FV
Sbjct:  230 SNCGARNFRLEALEALEKLNITIDSYGSCHRNRDGSTVNKLEALKRYKFSLAFENSNEEDYVTEKFFQSLVAGAIPVVIGAPNIQDFAPSPASLLHIGELSDVESVANSMKYLAANPEAFNNSLRWKYEGPSDSFKALFDMGAVHSSCRLCIHVATGIHETEEKGLAFKN----------RPCKCTRGSE--------TVHHLYVRERGRFNSESIFLRSSNLTVEGLASAVISKFNSLKHEPIWKQERPKSLKGGDE---------LRLYKIYPVGLTQRQALY-----TFRFKG----DSGFRRYVEDNPCAKLEAIFV 514          

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|1040896812|ref|XP_017256161.1| (PREDICTED: glycoprotein 3-alpha-L-fucosyltransferase A-like isoform X3 [Daucus carota subsp. sativus])

HSP 1 Score: 231.106 bits (588), Expect = 2.072e-68
Identity = 127/316 (40.19%), Postives = 184/316 (58.23%), Query Frame = 0

Query:   64 SNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQPD-IHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFL-TEPFTVATLHATLLALYGS-EHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            SNC A +FRL+A+   +K N+ + SYG C  N+   + K   L   KFSLAFENS E DYVTEKYFQ+L  G++PVV+GAPNI +++PS N+I+HI+ L  +P V+  M+ L     AY+  L WK  GPSD F ALVD+  V   CRLC+YLA R+R  +      +   D  +RPC C    E         ++H+YVRER  FE  S+FL +   T+  L + + + + S +H P+W  +RP   + + +         +K++++YP G+T ++ALY      FS  G    D  LR+ +++NPC + EV FV
Sbjct:  158 SNCAARNFRLQALEGLEKENIKIDSYGGCHRNRDGRVDKVQALKRYKFSLAFENSNEEDYVTEKYFQSLVAGSIPVVVGAPNIQEFSPSSNAILHIKELKDIPSVSKSMRYLADHPEAYNQSLRWKYEGPSDSFKALVDMAAVHSSCRLCIYLATRIRESD------EKSLDSSKRPCKCTRGSE--------TVYHVYVRERGRFEMVSIFLRSSNLTLKALESAVQSKFESLKHVPIWKDERPESIRGTDE---------LKVYRIYPLGLTQRQALY-----TFSFKG----DNELRKHLEKNPCAKFEVIFV 441          

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|586642191|ref|XP_006826237.1| (putative fucosyltransferase-like protein [Amborella trichopoda] >gi|548830481|gb|ERM93474.1| hypothetical protein AMTR_s00132p00113370 [Amborella trichopoda])

HSP 1 Score: 231.876 bits (590), Expect = 5.001e-68
Identity = 132/326 (40.49%), Postives = 190/326 (58.28%), Query Frame = 0

Query:   54 PASSFLFPHPSNCHANSFRLEAISFFQKHNVTVHSYGRC-LNNQPDIHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFL-TEPFTVATLHATLLALYGS-EHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            PA++F+    SNC A +FRLEA+   +K N+ + SYG C  N+   + K   L   KFSLAFENS E DYVTEK+FQ+L  G +PVV+GAPNI D+AP+  SI+HI+ +  +P VA  +  L ++  A++  L WK  GPSD F ALVD+  V   CRLC++LA ++R     + + ++  +  +RPC C          S   +HHL+VRER  FE  S+FL +   T+  L + +LA + S  H P+W   RPS  K   D         +KI+++YP G+T ++ALY      FS  G    D  L+ +++ NPC ++EV FV
Sbjct:  213 PAAAFI----SNCGARNFRLEALELLEKMNIQIDSYGGCHRNHDGRVDKVKALKRYKFSLAFENSNEEDYVTEKFFQSLVAGTIPVVVGAPNIQDFAPAPGSILHIKEVDDVPSVAKNITYLASNPVAFNNSLRWKYEGPSDSFKALVDMAAVHSSCRLCIHLATQIR-----EKKERSNPNFQKRPCNCTR--------SGVTVHHLFVRERGRFEMESIFLSSSSLTLQALESAVLAKFKSLNHVPIWKGDRPSVIKGGDD---------LKIYRIYPLGMTQRQALY-----TFSFKG----DGDLQYYIESNPCAKLEVIFV 503          

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|1201316285|gb|OVA15676.1| (Glycosyl transferase [Macleaya cordata])

HSP 1 Score: 233.032 bits (593), Expect = 6.420e-68
Identity = 135/332 (40.66%), Postives = 190/332 (57.23%), Query Frame = 0

Query:   49 PSPPLPASSFLFPHPSNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQPD--IHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFL-TEPFTVATLHATLLALYGS-EHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYGQIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            P  P   ++      SNC A +FRL+A+   +K NV + SYG C  N+    + K D L H KFSLAFENS E DYVTEK+FQ+L  G+VPVV+GAPNILD+APS  S++HI+ L  +P VA  M+ L  +  AY+  + WK  GPSD F ALVD+  V   CRLC++LA ++   E  +   K      +RPC C    E         ++HLYVRER  F+  S+FL +   T+  L + +LA + S  H P+W ++RP  R +  D         +K++++YP G+T ++ALY      F   G    D  LR ++++NPC + EV FV
Sbjct:  249 PLQPKTEAALAAAFISNCGARNFRLQALEMLEKSNVKIDSYGGCHRNRDGRAVDKVDALKHYKFSLAFENSNEEDYVTEKFFQSLVAGSVPVVVGAPNILDFAPSPGSVLHIKELDDVPSVAKTMKYLAENPDAYNQTVRWKYDGPSDSFKALVDMAAVHSSCRLCIHLATKIWETEERRPGLK------KRPCKCTRGLE--------TVYHLYVRERGRFQMDSIFLRSGNVTLEALESVVLAKFKSLNHVPIWKQERPE-RIRGGD--------ELKVYRIYPVGMTQRQALY-----TFRFKG----DSDLRSYLERNPCAKFEVIFV 548          

BLAST of EWM21269.1 vs. NCBI_GenBank
Match: gi|1147613923|ref|XP_020110975.1| (glycoprotein 3-alpha-L-fucosyltransferase A-like [Ananas comosus])

HSP 1 Score: 231.106 bits (588), Expect = 6.894e-68
Identity = 129/332 (38.86%), Postives = 191/332 (57.53%), Query Frame = 0

Query:   49 PSPPLPASSFLFPHPSNCHANSFRLEAISFFQKHNVTVHSYGRCLNNQPD-IHKDDRLPHVKFSLAFENSEEVDYVTEKYFQALNFGAVPVVIGAPNILDYAPSENSIIHIENLAALPRVAAQMQALMADDHAYDAMLAWKARGPSDQFLALVDVGTVAPECRLCLYLADRVRRGEAFKDRAKAKEDGYRRPCACRPQEEGGEGGSNGVIHHLYVRERFTFEYRSLFL-TEPFTVATLHATLLALYGS-EHRPVWTRKRPSYRKKSPDGSWDMAYVPIKIHKVYPAGITTKEALYG-QIPDAFSVVGNRTMDQALRRWVKQNPCGEIEVTFV 376
            P  P   ++      SNC A +FRL+A+   ++  + + SYG C  N+   + K + L   KFSLAFENS E DYVTEK+FQ+L  G+VPVV+GAPNI D+AP+  SI+HI+ L+ +  +A  M+ L AD  AY+  + WK  GPSD F ALVD+  V   CRLC++LA ++R  E  +  AK +     RPC+C          + G ++HL+VRER  FE  S+FL ++  TV  L   +L  + S  + P+W  +RP+  +   +         +KI+++YP G+T ++ALY  +  D          D  L+R++K NPC ++EV FV
Sbjct:  191 PMQPKTEAALAAAFISNCGARNFRLQALEMLEQMGIKIDSYGACHRNRDGRVDKVEALKKYKFSLAFENSNEEDYVTEKFFQSLVAGSVPVVVGAPNIQDFAPAPGSILHIKELSDVSSIAQTMKHLAADPDAYNRTMRWKFEGPSDSFKALVDMAAVHSSCRLCIHLATKIREKE--EKTAKFQN----RPCSC--------SSNKGTVYHLFVRERGRFEMESIFLRSDKLTVEALEFAVLEKFRSLNYVPIWKNERPASIRGGDE---------LKIYRIYPVGLTQRQALYSFKFKD----------DADLKRYIKNNPCTKLEVIFV 489