EWM21649.1, cds9071 (CDS) Nannochloropsis gaditana

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|585101904|gb|EWM21649.1| (24-dehydrocholesterol reductase [Nannochloropsis gaditana])

HSP 1 Score: 1254.58 bits (3245), Expect = 0.000e+0
Identity = 604/604 (100.00%), Postives = 604/604 (100.00%), Query Frame = 0

Query:    1 MPQQSKLVSRGQASAREENWFEYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERESRKGVRRPALAGTQVALSEFAEGRAVEPRGGKEVNGGTGGPTGKGEGDVPSPSSTSGGRVGRREDAGGEDAADFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAARV 604
            MPQQSKLVSRGQASAREENWFEYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERESRKGVRRPALAGTQVALSEFAEGRAVEPRGGKEVNGGTGGPTGKGEGDVPSPSSTSGGRVGRREDAGGEDAADFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAARV
Sbjct:    1 MPQQSKLVSRGQASAREENWFEYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERESRKGVRRPALAGTQVALSEFAEGRAVEPRGGKEVNGGTGGPTGKGEGDVPSPSSTSGGRVGRREDAGGEDAADFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAARV 604          

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|919065762|ref|XP_013414441.1| (PREDICTED: delta(24)-sterol reductase-like [Lingula anatina])

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 281/583 (48.20%), Postives = 375/583 (64.32%), Query Frame = 0

Query:   21 FEYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERESRKGVRRPALAGTQVALSEFAEGRAVEPRGGKEVNGGTGGPTGKGEGDVPSPSSTSGGRVGRREDAGGEDAADFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAAR 603
             EY++ ++RWVF  F L+P+S+++D+  ++RN + F + N APK HD KV+D+++Q+++WA    + ++CTARPGW T+S R G YK+T R I ++L DIL ++T+     VRVEPL TMGQ+TA L  LGWT+PVLPELDDLTVGGLI GVG+E+SSH YGLFQH C+ FE+ LADGSVV CS ++NP+LFY +PWS+GTLGFL+SA+I+IIPAK Y++++Y    +  E L +FE E++K      + G       ++E  AV               TG    D PS    S G                                                    FWKPWFFKHVE FL  G            G EYIPLRHYYHRH++S+FWE+QDIIPFGNHP+FRYLFGWM+PP+ISLLKLTQ E ++++YE+H ++QDMLVP+ DLG +L CF +   +YPLWLCP  +P  P      G + P  D D M+VD+GAYG P +  F+  +  R  E+FVR  +G+QM+YADS+M+REEFREMFDH  YD++R +++ C +AFPEVYDKV+K AR
Sbjct:   24 LEYILINYRWVFVCFFLLPISVIYDVFYFVRNWIVFKL-NSAPKLHDKKVQDVQRQVKQWASDGCQTQMCTARPGWMTVSFRQGLYKRTMRNINVNLIDILEVDTTN-RQVVRVEPLATMGQITATLNPLGWTLPVLPELDDLTVGGLIMGVGIETSSHKYGLFQHCCVAFELVLADGSVVKCSKDENPDLFYAVPWSYGTLGFLVSADIKIIPAKKYLRMEYKPVHSFNEILRVFEEETKKKTGNEFVEGLM-----YSENEAVI-------------MTGNMTNDAPSDKINSIG---------------------------------------------------NFWKPWFFKHVEGFLRTGP-----------GVEYIPLRHYYHRHTRSIFWELQDIIPFGNHPVFRYLFGWMVPPKISLLKLTQGETIKRMYEQHQMIQDMLVPMADLGSALKCFHKEVKLYPLWLCPFYLPNLP------GMVHPKGDKDAMYVDIGAYGEPKVNNFDPVKTTRNIEEFVRKVQGFQMLYADSYMTREEFREMFDHTLYDKMRGQLN-CKKAFPEVYDKVNKKAR 517          

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|944209713|gb|KQK76604.1| (delta(24)-sterol reductase [Amazona aestiva])

HSP 1 Score: 552.747 bits (1423), Expect = 0.000e+0
Identity = 287/583 (49.23%), Postives = 369/583 (63.29%), Query Frame = 0

Query:   21 FEYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERESRKGVRRPALAGTQVALSEFAEGRAVEPRGGKEVNGGTGGPTGKGEGDVPSPSSTSGGRVGRREDAGGEDAADFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAAR 603
             E ++ HHRWVF    L+PLS++FD+   +R    + + + AP+ H  +V  I+ Q+REW     K  +CT  PGW T+SLRVGKYKKT + I ++L D+L +++      VRVEPLVTMGQLTA L  +GWT+PV+PELDDLTVGGLI G G+ESSSHIYGLFQH C+ +E+ LADGS+V CSP +N +LFY +PWS GTLGFL++AEI++IPAK  VK+ Y                  K VR     G Q    EF E                                         E    E++  FVE L +S E  V+M G + D+     E  K+N IG ++KPWFFKHVE +L         KD +  G EYIP RHYYHRH++S+FWE+QDIIPFGNHP+FRYLFGWM+PP+ISLLKLTQ EA+RKLYE+HHVVQDMLVP+K L +S+  F +  +VYPLWLCP  +P NP      G + P  D  E++VD+GAYG P  + F AR + R+ E FVR+  G+QM+YAD +M+REEF +MFD   Y +LR +M+ C  AFPEVYDK+ KAAR
Sbjct:   23 LEAVLVHHRWVFVCLFLLPLSILFDVYYQLRAWAVWRLHS-APRLHAQRVRHIQAQVREWKDEGSKRYMCTGXPGWLTVSLRVGKYKKTHKNIMINLMDVLEVDSERQV--VRVEPLVTMGQLTAYLNPMGWTIPVVPELDDLTVGGLIMGTGIESSSHIYGLFQHTCVAYELVLADGSLVRCSPTENSDLFYAVPWSCGTLGFLVAAEIKMIPAKKXVKIHY------------------KPVR-----GLQNVCEEFTE-----------------------------------------ESKKKENS--FVEGLVYSLEEAVIMTGVLTDEA----EPSKINRIGNYYKPWFFKHVEKYL---------KD-DRTGTEYIPARHYYHRHTRSIFWELQDIIPFGNHPVFRYLFGWMVPPKISLLKLTQGEAIRKLYEQHHVVQDMLVPMKSLEKSIQTFHDDLNVYPLWLCPFILPNNP------GMVHPKGDEAELYVDIGAYGEPKRKQFEARASMRQMEKFVRSVHGFQMLYADCYMTREEFWDMFDGSXYHKLREQMN-CKDAFPEVYDKICKAAR 515          

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|291242187|ref|XP_002740991.1| (PREDICTED: delta(24)-sterol reductase [Saccoglossus kowalevskii])

HSP 1 Score: 340.887 bits (873), Expect = 7.738e-107
Identity = 161/283 (56.89%), Postives = 204/283 (72.08%), Query Frame = 0

Query:  322 REDAGGEDAADFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAARV 604
            ++   GE+  +FVEAL +S    V+M        T   EA KVNAIG +WKPWFFKHVES+L KG            G EYIPLRHYYHRH++S+FWE+QDIIPFGN+P+FRYL GWM+PP+ISLLKLTQ E +R+LYE+HHV+QDMLVP++ L  SL CF++   +YPLW+CPM +P  P      G + P  D DEMFVD+GAYG P  +GF AR+  R+ E +VR+  G+QM+YAD +M++EEFR MFDH  YD LR K++ C +AFPEVYDKVSKAAR+
Sbjct:  258 KDKVFGEN--EFVEALVYSDHEAVIMTANQ----TENAEAEKVNAIGNYWKPWFFKHVESYLKKGRG----------GVEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLCGWMVPPKISLLKLTQGETIRRLYEQHHVIQDMLVPLETLDSSLKCFDKELKLYPLWVCPMLLPSIP------GMVHPKSDQDEMFVDIGAYGEPKRDGFKARDTVRRLEKYVRSVDGFQMLYADCYMTKEEFRFMFDHSLYDRLRTKLN-CCKAFPEVYDKVSKAARL 517          

HSP 2 Score: 283.493 bits (724), Expect = 9.411e-85
Identity = 130/237 (54.85%), Postives = 178/237 (75.11%), Query Frame = 0

Query:   21 FEYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFE 257
             EY++ H RW+F    L+P+S++FD+ +  RN + F M + APK HD +V DI+KQ+REW     +  +CTARPGW T+S RV KYK T RKI+++L D+L I+T     +VRVEPL TMGQ+TA L  LGWT+PVLPELDDLTVGGLI GVG+E+SSH +GLFQH C+ +E+ LADGS+V CS +++P+LFY +PWS+GTLGFL++AEI+IIPAK +VK++Y+     +    +F+
Sbjct:   25 LEYVVVHQRWIFVCLFLLPVSVLFDVLISFRNWITFKMTS-APKKHDTRVRDIQKQVREWMTDGCEQPMCTARPGWMTVSPRVAKYKHTHRKIQVNLIDVLEIDTE--RQTVRVEPLATMGQITATLNPLGWTLPVLPELDDLTVGGLIMGVGIETSSHKHGLFQHTCLSYELVLADGSLVKCSKDEDPDLFYAVPWSYGTLGFLVAAEIKIIPAKKFVKIEYFPVKTTENISKVFK 258          

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|390343862|ref|XP_792094.3| (PREDICTED: delta(24)-sterol reductase [Strongylocentrotus purpuratus])

HSP 1 Score: 336.265 bits (861), Expect = 5.892e-105
Identity = 156/272 (57.35%), Postives = 199/272 (73.16%), Query Frame = 0

Query:  332 DFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAAR 603
            +FVE L +SK+  V+M G + D+     E  K+NAIG FWKPWFFKHVESFL  G +            EYIPLRHYYHRHS+S+FWEIQDI+PFGNHP+FRYL GW+ PP++SLLKLTQ E +R+LYE+ HVVQDMLVP+KD+  SL CF +   +YPLWLCP  +P  P      G + PL + +E+FVD+GAYGNP    F+ R++ RK E+ VR   G+QM+YADS+M+REEFREMFDH  YD+LR+ + +C  AFPEVYDK++K+AR
Sbjct:  274 EFVEGLMYSKDKAVIMTGQLTDQA----EPAKINAIGNFWKPWFFKHVESFLKTGPAV-----------EYIPLRHYYHRHSRSIFWEIQDIVPFGNHPIFRYLLGWLTPPKVSLLKLTQGEIIRELYEKKHVVQDMLVPLKDMKSSLMCFHDEMEMYPLWLCPFVLPALP------GMVHPLGNQEELFVDIGAYGNPKNPNFHFRDSTRKVEEHVRKVNGFQMLYADSYMTREEFREMFDHSLYDKLRKNL-KCEGAFPEVYDKINKSAR 523          

HSP 2 Score: 282.722 bits (722), Expect = 1.869e-84
Identity = 134/242 (55.37%), Postives = 180/242 (74.38%), Query Frame = 0

Query:   22 EYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERESRKG 263
            EY+I   RW+F    L+PLS ++D+  ++RN + F M + APK H  +V+DI+ Q+++W     +  +CT RPGWQT+SLRVGKYK T RKI ++L DIL I+T     +++VEPL TMGQ+TA L  LGWT+PVLPELDDLTVGGLI GVG+ESSSH YGLFQH+C+ FE+ LADGSV  CS ++NPELFY++PWS+GTLGFL+SAEI+I+PAK YV+L+Y       +   +F + S++ 
Sbjct:   32 EYVIVKQRWIFVCLFLLPLSAVYDVYYFMRNWVVFRMSS-APKLHAERVQDIQSQVKKWKSDKSQQLMCTGRPGWQTISLRVGKYKLTHRKIFINLVDILDIDTE--RQTMKVEPLATMGQITAMLNPLGWTLPVLPELDDLTVGGLIMGVGIESSSHKYGLFQHVCVSFELVLADGSVAQCSKDENPELFYSVPWSYGTLGFLVSAEIKIVPAKQYVRLEYKPVHCFDDVSNVFAKCSKEA 270          

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|156370088|ref|XP_001628304.1| (predicted protein [Nematostella vectensis] >gi|156215277|gb|EDO36241.1| predicted protein [Nematostella vectensis])

HSP 1 Score: 330.872 bits (847), Expect = 5.958e-103
Identity = 164/285 (57.54%), Postives = 207/285 (72.63%), Query Frame = 0

Query:  322 REDAGGEDAADFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFI--RPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAARV 604
            RE    E+  +FVEALA+S+   VVM GT  D+     E  K+N IG FWKPWFFKHV+S+L  G            G EYIPLR YYHRH++S+FWE+QDIIPFGN+ ++RYLFGW+MPP+ISLLKLTQ E LR+LYE+HHVVQDMLVP++ LG SL CF E F +YPLW+CPM +P+ P    H      RP  DG  M+VD+GAYG P ++ F ARE  R+ E FV++  G+QM+YADS+M+REEFR MFDH  YD+LR +M +C +AFPE+YDKVS+AAR+
Sbjct:  263 RESMSKEN--EFVEALAYSEIEAVVMTGTFEDEA----EPDKLNPIGYFWKPWFFKHVQSYLKSGP-----------GVEYIPLRDYYHRHTRSIFWELQDIIPFGNNVIWRYLFGWVMPPKISLLKLTQGETLRRLYEQHHVVQDMLVPIETLGNSLSCFHENFELYPLWICPMYLPRTPGL-VHAPVDKRRPHSDG-RMYVDIGAYGTPKVKNFVARENMRRVEAFVKSVDGFQMLYADSYMTREEFRAMFDHELYDKLREEM-QCKEAFPEIYDKVSRAARL 527          

HSP 2 Score: 271.166 bits (692), Expect = 5.367e-80
Identity = 132/241 (54.77%), Postives = 176/241 (73.03%), Query Frame = 0

Query:   22 EYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPS--VRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERES 260
            E++I H RW+F    L+P+S++++  L++RN L F   +  P+ H  +V+ +++Q+++W     K  +CTARPGW T+S RVGKYKKT   I ++L DIL IN +    +  VRVEP+VTMGQLT AL+ LGWT+PVLPELDDLTVGGLI G G+ESSSH +GLFQHIC+ F++ LADG++V  S ++N  LFY+IPWSHGTLGFL++AEI IIPAK YV+L Y    + KE  T F RES
Sbjct:   26 EHIIIHQRWIFVCLFLLPVSVLYESFLFVRNWLAFKYYS-DPERHKERVQKVQEQVQKWNKDGRKQPMCTARPGWMTVSPRVGKYKKTHCNIDVNLMDILEINCAAGESTGVVRVEPMVTMGQLTHALVPLGWTIPVLPELDDLTVGGLIMGCGIESSSHKFGLFQHICVGFDLVLADGTLVHASKKENEHLFYSIPWSHGTLGFLVAAEIIIIPAKKYVRLSYNPVHSKKELETSFTRES 265          

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|1191024527|ref|XP_020896413.1| (delta(24)-sterol reductase-like isoform X1 [Exaiptasia pallida] >gi|999981682|gb|KXJ20856.1| Delta(24)-sterol reductase [Exaiptasia pallida])

HSP 1 Score: 322.783 bits (826), Expect = 7.645e-100
Identity = 153/274 (55.84%), Postives = 195/274 (71.17%), Query Frame = 0

Query:  332 DFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGD-EMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAARV 604
            DFVE L +S+   VVM G   D+P    E  K+NAIG FWKPWFFKHVE +L        GK       EYIPLR YYHRH++SLFWE+QDI+PFGN+ ++RYL GW MPP+ISLLKLTQ E LR+LYE+HHVVQDMLVP+  L ++L CF E F +YPLWLCPM +P  P      G +    +GD +M+VDVGAYG P   GF AR + R  E  VR  +GYQM+YADS+M+R+EF +MFDH  Y+++R+ ++ C +AFPE+YDKVS++AR+
Sbjct:  271 DFVEGLVYSENEAVVMTGKFCDEP----EPDKINAIGHFWKPWFFKHVEQYLT-------GKPTLT---EYIPLRDYYHRHTRSLFWELQDIVPFGNNVIWRYLMGWSMPPKISLLKLTQGETLRRLYEQHHVVQDMLVPMTTLDKALTCFHEEFQLYPLWLCPMYLPNTP------GLVHSHGEGDGKMYVDVGAYGTPHANGFKARNSLRNVEAIVRKLQGYQMLYADSYMTRDEFHQMFDHTLYNKMRKTLN-CEEAFPEIYDKVSRSARI 523          

HSP 2 Score: 269.24 bits (687), Expect = 2.820e-79
Identity = 129/239 (53.97%), Postives = 174/239 (72.80%), Query Frame = 0

Query:   22 EYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERES 260
            E++I + RW+F    L+PLS++++I L +RN L F   + +P+ H  +V  +++Q+  W        +CTARPGW T+SLRVGKYK+T  KI+++L D+L ++T      VRVEPLVTMGQLTA LL LGWTVPVLPELDDLTVGGLI G GVESSSH YG+FQH C+ +++ LADGS+   S E+N +LFY+IPWSHGTLGFL++AEI+IIPAK YV L Y+   + +  +  F ++S
Sbjct:   29 EHVIINQRWIFVCLFLLPLSVLYEIFLSVRNWLAFKYYS-SPERHAKRVRYVQEQVTSWNKKGRVKPMCTARPGWMTVSLRVGKYKETHEKIQVNLMDVLEVDTERSV--VRVEPLVTMGQLTATLLPLGWTVPVLPELDDLTVGGLIMGCGVESSSHKYGMFQHTCVAYDIVLADGSLAHASKEENTDLFYSIPWSHGTLGFLVAAEIKIIPAKKYVCLTYHPIHSKEHLIATFTKKS 264          

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|704564372|ref|XP_010183462.1| (PREDICTED: delta(24)-sterol reductase [Mesitornis unicolor])

HSP 1 Score: 317.775 bits (813), Expect = 3.328e-99
Identity = 151/271 (55.72%), Postives = 193/271 (71.22%), Query Frame = 0

Query:  333 FVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAAR 603
            FVE L +S E  V+M G + D+P    E  K+N IG ++KPWFFKHVE +L            +  G EYIP RHYYHRH++S+FWE+QDIIPFGN+P+FRYLFGWM+PP+ISLLKLTQ EA+RKLYE+HHVVQDMLVP+K L +S+  F +  +VYPLWLCP  +P NP      G + P  D  E++VD+GAYG P  + F AR + R+ E FVR+  G+QM+YAD +M+REEF +MFD   Y +LR +M+ C  AFPEVYDK+ KAAR
Sbjct:  177 FVEGLMYSLEEAVIMTGVLTDEP----EQSKINRIGNYYKPWFFKHVEMYLK----------ADRTGIEYIPARHYYHRHTRSIFWELQDIIPFGNNPVFRYLFGWMVPPKISLLKLTQGEAIRKLYEQHHVVQDMLVPMKSLEKSIQTFHDDLNVYPLWLCPFILPNNP------GMVHPKGDEAELYVDIGAYGEPKRKQFEARASMRQMEKFVRSVHGFQMLYADCYMTREEFWDMFDGSLYHKLRDQMN-CKDAFPEVYDKICKAAR 426          

HSP 2 Score: 221.476 bits (563), Expect = 2.502e-62
Identity = 105/174 (60.34%), Postives = 132/174 (75.86%), Query Frame = 0

Query:   89 LCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERESRK 262
            +CT RPGW T+SLRVGKYKK  + I ++L D+L +++      V VEPLVTMGQLTA L  +GWT+PV+PELDDLTVGGLI G G+ESSSHIYGLFQH CM +E+ LADGS+V CSP +N +LFY +PWS GTLGFL++AEI++IPAK YVK+ Y      ++    F  ES+K
Sbjct:    1 MCTGRPGWLTVSLRVGKYKKIHKNIMINLMDVLEVDSERQV--VLVEPLVTMGQLTAYLNPMGWTIPVVPELDDLTVGGLIMGTGIESSSHIYGLFQHTCMAYELVLADGSLVRCSPTENSDLFYAVPWSCGTLGFLVAAEIKMIPAKKYVKIHYEPVRGLQKICEKFTEESKK 172          

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|677438790|gb|KFQ36371.1| (Delta(24)-sterol reductase, partial [Mesitornis unicolor])

HSP 1 Score: 317.775 bits (813), Expect = 5.351e-99
Identity = 151/271 (55.72%), Postives = 193/271 (71.22%), Query Frame = 0

Query:  333 FVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAAR 603
            FVE L +S E  V+M G + D+P    E  K+N IG ++KPWFFKHVE +L            +  G EYIP RHYYHRH++S+FWE+QDIIPFGN+P+FRYLFGWM+PP+ISLLKLTQ EA+RKLYE+HHVVQDMLVP+K L +S+  F +  +VYPLWLCP  +P NP      G + P  D  E++VD+GAYG P  + F AR + R+ E FVR+  G+QM+YAD +M+REEF +MFD   Y +LR +M+ C  AFPEVYDK+ KAAR
Sbjct:  190 FVEGLMYSLEEAVIMTGVLTDEP----EQSKINRIGNYYKPWFFKHVEMYLK----------ADRTGIEYIPARHYYHRHTRSIFWELQDIIPFGNNPVFRYLFGWMVPPKISLLKLTQGEAIRKLYEQHHVVQDMLVPMKSLEKSIQTFHDDLNVYPLWLCPFILPNNP------GMVHPKGDEAELYVDIGAYGEPKRKQFEARASMRQMEKFVRSVHGFQMLYADCYMTREEFWDMFDGSLYHKLRDQMN-CKDAFPEVYDKICKAAR 439          

HSP 2 Score: 231.491 bits (589), Expect = 6.739e-66
Identity = 110/187 (58.82%), Postives = 138/187 (73.80%), Query Frame = 0

Query:   76 QIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERESRK 262
            Q+REW     K  +CT RPGW T+SLRVGKYKK  + I ++L D+L +++      V VEPLVTMGQLTA L  +GWT+PV+PELDDLTVGGLI G G+ESSSHIYGLFQH CM +E+ LADGS+V CSP +N +LFY +PWS GTLGFL++AEI++IPAK YVK+ Y      ++    F  ES+K
Sbjct:    1 QVREWKDEGSKRYMCTGRPGWLTVSLRVGKYKKIHKNIMINLMDVLEVDSERQV--VLVEPLVTMGQLTAYLNPMGWTIPVVPELDDLTVGGLIMGTGIESSSHIYGLFQHTCMAYELVLADGSLVRCSPTENSDLFYAVPWSCGTLGFLVAAEIKMIPAKKYVKIHYEPVRGLQKICEKFTEESKK 185          

BLAST of EWM21649.1 vs. NCBI_GenBank
Match: gi|847118359|ref|XP_012816582.1| (PREDICTED: delta(24)-sterol reductase isoform X1 [Xenopus tropicalis])

HSP 1 Score: 319.701 bits (818), Expect = 8.479e-99
Identity = 154/282 (54.61%), Postives = 197/282 (69.86%), Query Frame = 0

Query:  322 REDAGGEDAADFVEALAFSKEHVVVMVGTMVDKPTGRREAGKVNAIGKFWKPWFFKHVESFLAKGSSEMKGKDVEAVGEEYIPLRHYYHRHSKSLFWEIQDIIPFGNHPLFRYLFGWMMPPRISLLKLTQTEALRKLYEEHHVVQDMLVPVKDLGESLDCFEEVFSVYPLWLCPMRIPKNPDFRKHGGFIRPLEDGDEMFVDVGAYGNPSMEGFNAREACRKAEDFVRAKKGYQMMYADSFMSREEFREMFDHGKYDELRRKMDRCLQAFPEVYDKVSKAAR 603
            RE    E+   FVE L +S +  V+M G + D+     +AG+VN IG++WKPWFF+HVES+L               G EYIPLRHYYHRH+KS+FWE+QDIIPFGNHP+FRY FGWM+PP+ISLLKLTQ E +RKLYE+HHVVQDMLVP+K L +++  F    SVYPLWLCP  +P +P      G I P  +  E++VD+GAYG P  + F+A+ + R+ E FVR   G+QM+YAD +MSREEF +MFD   Y +LR K++ C  AFPEVYDK+ KAAR
Sbjct:  257 RESKNKENY--FVEGLVYSADEAVIMTGVLTDEA----QAGQVNRIGQYWKPWFFRHVESYLKNNRD----------GTEYIPLRHYYHRHTKSIFWELQDIIPFGNHPVFRYFFGWMVPPKISLLKLTQGETIRKLYEQHHVVQDMLVPMKCLQKAITAFHSEISVYPLWLCPFILPSHP------GMIHPKGNEAELYVDIGAYGEPKTKHFDAKGSMRRLEKFVRDVHGFQMLYADCYMSREEFWDMFDGALYQKLRGKLN-CNNAFPEVYDKICKAAR 515          

HSP 2 Score: 290.812 bits (743), Expect = 1.181e-87
Identity = 138/242 (57.02%), Postives = 178/242 (73.55%), Query Frame = 0

Query:   21 FEYMITHHRWVFCIFLLMPLSLMFDIALYIRNGLQFYMRNWAPKHHDAKVEDIKKQIREWALHDGKNKLCTARPGWQTMSLRVGKYKKTFRKIKLDLHDILSINTSGPAPSVRVEPLVTMGQLTAALLSLGWTVPVLPELDDLTVGGLIAGVGVESSSHIYGLFQHICMQFEVALADGSVVFCSPEKNPELFYNIPWSHGTLGFLLSAEIRIIPAKPYVKLQYYAFTNGKEALTMFERESRK 262
            FEY+I HHRW+F    L+PLS++FD+  Y R  L F M + APK HD +V+DI+ Q+R+W    GKN +CT RPGW T+SLRVGKYK T + I ++L DIL ++T      VRVEPLV MGQ+TA L S+ WT+PV+PELDDLTVGGLI G G+ESSSH +GLFQHIC+ +E+ LADGS+V C+P +N +LFY +PWS GTLGFL++AEI+I+PAK YVKL Y      ++    F RES+ 
Sbjct:   23 FEYVIVHHRWIFVCLFLLPLSVIFDVYYYARAWLVFKMCS-APKQHDRRVKDIQDQVRQWKAEGGKNFMCTGRPGWLTVSLRVGKYKNTHKNIMINLMDILEVDTKRQV--VRVEPLVNMGQVTALLNSISWTLPVVPELDDLTVGGLIMGTGIESSSHNFGLFQHICLAYELVLADGSLVRCTPTENSDLFYAVPWSCGTLGFLVAAEIKIVPAKKYVKLHYTPVKGLEKICEKFSRESKN 261