EWM23961.1, cds6683 (CDS) Nannochloropsis gaditana

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|585105247|gb|EWM23961.1| (p-type atpase [Nannochloropsis gaditana])

HSP 1 Score: 2445.23 bits (6336), Expect = 0.000e+0
Identity = 1185/1185 (100.00%), Postives = 1185/1185 (100.00%), Query Frame = 0

Query:    1 MATEVELGKSKKSAASNGKKNGADEKPEVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLDCRTRTTEYMTREGNVDKWDSFKELVTAGALCNMCKYEADPNAPAVAAAGSLDLSQATNRPSIATMNNYTLSKRPAAVAGPLGNHQMTYQGTLARRSMQQQKGEAAKSTILNPGFFDRGAAALEASTGAGAGAGADTVRGMANLPNIVRQLSSVTGQATVGRGSQATFGRNSQATFGRNSQALQGAGTLNRLSVNALGRNTFLGGLPLVEAPPRIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPPTQADQRRVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDLFQFGVTDDFALRDGRVLSLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRKFLIRVYPTSCFANAFAF 1185
            MATEVELGKSKKSAASNGKKNGADEKPEVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLDCRTRTTEYMTREGNVDKWDSFKELVTAGALCNMCKYEADPNAPAVAAAGSLDLSQATNRPSIATMNNYTLSKRPAAVAGPLGNHQMTYQGTLARRSMQQQKGEAAKSTILNPGFFDRGAAALEASTGAGAGAGADTVRGMANLPNIVRQLSSVTGQATVGRGSQATFGRNSQATFGRNSQALQGAGTLNRLSVNALGRNTFLGGLPLVEAPPRIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPPTQADQRRVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDLFQFGVTDDFALRDGRVLSLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRKFLIRVYPTSCFANAFAF
Sbjct:    1 MATEVELGKSKKSAASNGKKNGADEKPEVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLDCRTRTTEYMTREGNVDKWDSFKELVTAGALCNMCKYEADPNAPAVAAAGSLDLSQATNRPSIATMNNYTLSKRPAAVAGPLGNHQMTYQGTLARRSMQQQKGEAAKSTILNPGFFDRGAAALEASTGAGAGAGADTVRGMANLPNIVRQLSSVTGQATVGRGSQATFGRNSQATFGRNSQALQGAGTLNRLSVNALGRNTFLGGLPLVEAPPRIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPPTQADQRRVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDLFQFGVTDDFALRDGRVLSLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRKFLIRVYPTSCFANAFAF 1185          

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|189086526|gb|ACD75720.1| (putative type 2C P-type ATPase [Plasmodiophora brassicae])

HSP 1 Score: 705.286 bits (1819), Expect = 0.000e+0
Identity = 439/1164 (37.71%), Postives = 653/1164 (56.10%), Query Frame = 0

Query:   37 SFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLDCRTRTTEYMTREGNVDKWDSFKELVTAGALCNMCKYEADPNAPAVAAAGSLDLSQATNRPSIATMNNYTLSKRPAAVAGPLGNHQMTYQGTLARRSMQQQKGEAAKSTILNPGFFDRGAAALEASTGAGAGAGADTVRGMANLPNIVRQLSSVTGQATVGRGSQATFGRNSQATFGRNSQALQGAGTLNRLSVNALGRNTFLGGLPLVEAPPR---IIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPP---TQADQRRVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASV---DDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDL-----FQFGVTDDFALRDGRVLSLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAF-SAYGRQPIQFFLFFLVSGAILVGWGELRKFLIRVYPTSCFANAFAF 1185
            S +EL       +N +    S+GL   +A++RL  +G N +TP P  P +       LDP ++LL+   VL      T   +  +  +LY+ ++L  ++  T+ LSF+QEGQ+   M  F+ MM ++A VRRGG +  + A DLV GD+V++ +G RVPAD+ ++ +  L+++ SS TGE  PV+MC     +     +++A + + V+ G G GIVVRTGD   IG IASL  Q    +STL   V  FV LI   AI+ A   + V +I+       G  +  +  ++ FIV+L+ANVPEGLPA V+ CL V A+R+A + V VKRL+ ++TLG+ T + +DKTGTLTQN M V+ L+ +   +  + ++   ++ +  S ++L     L ++C +  DP    +  +   + + AT+   I         + PA         Q   +  + +R+   + G+A       P     G+  L      G    +D V    + P ++  +       T+ R      G+       R       A    R   +AL + T +         PR   ++ GN SD A+F F   + D+   R+E   ++++PF+S  K+M TIH  P    T    + ++I+KGA E +L  C   + KG  +P+        E    + A +GERVL  A   LP      S    +D+  +++     +  T +  ++L DPPK GV DAVL+C+ AGIRV MITGDHP TAEAIA+ VNII+ Y  R++IA    +  +   E   GAAV+T  ++E   ++ W+ LL   E+VFAR SP +KL+I   L+A+ EV AMTGDGVNDSPAL+ ADIGIAMG+ G +VA+ AA+VVL+DDDFC+IVNGIEEGR++FDNL K IAYTLTHLV E+ A+ I +   +P G++S MILSIDL TEV P++S ++E AE  +M RPPR+ KVDRLV   L  Y Y+ AG+  A + + AY +V+ S+G+   +L     FQ G      L    V S  +Q  I++SA +AF  T++LSQ  ++F  KTR   + EHGVFNN +MN GV+VE+G++IAIV++P +N AF +AYG Q  + +   LVS   L G+ E RK+++R   TS F++   +
Sbjct:   28 SLDELVKRTRTSVNTSDLDVSSGLKPEEAEERLRNDGKNCMTPKPPVPLWKRFGKHLLDPMLVLLDFAGVLSI---ATALSTPGDTTDLYLGILLFILVVCTAGLSFFQEGQAESAMQGFQKMMPQNACVRRGGVQTTINAVDLVRGDIVILSTGDRVPADVALLTARGLRIDMSSMTGESLPVKMC--SILEPPAAGESIALSGSQVMAGNGTGIVVRTGDRCTIGQIASLANQASTTESTLRRNVEHFVKLIAALAIVMAAAMFIVTIIQQ------GTKNILQRIVSSFIVVLVANVPEGLPAAVSGCLAVAARRMAAKNVLVKRLEVLDTLGAVTTICTDKTGTLTQNHMAVACLFYEGEVQPADSLSHPRHLKELKSAEQL---HGLFDIC-FSNDPLHCTIVFSALCNQATATDFVQI--------EEAPAETKLTAARTQ-DVRIDMVKRAGSGRTGKA-------PDAERHGSLVL------GMAESSDNVARFKSSPVLIDDV-------TIKRVRTMPIGKQDFTNINRGE-----APVPTRGKADALRKQTRV---------PRERDVVAGNASDVAMFCFAERYVDVMELRQEIKTIFMVPFSSERKWMATIHGVPAADRTNRTSKHLLIVKGALERILPKCKLVLKKGAVVPLTPDAIAEVESACGDFARRGERVLVMAMATLPAQPDSFSYTFEEDEAGNIKSNFTIDNLTLISCLALHDPPKVGVADAVLKCQRAGIRVTMITGDHPATAEAIARHVNIIKHYHTREEIAAMNLDGQSGDVE-LAGAAVVTGAEMESMTDQQWDELLSMHEIVFARTSPMNKLEIVTRLQAKGEVCAMTGDGVNDSPALRRADIGIAMGLRGHDVARDAADVVLVDDDFCTIVNGIEEGRLIFDNLRKIIAYTLTHLVAEVAAIAITLLAGLPPGLNSFMILSIDLGTEVCPSLSLSYETAEDAIMQRPPRDDKVDRLVSIPLFLYMYVWAGIPVAMLSIGAYLLVFQSHGIPLSELVHTSYFQPGCGP--LLIGSTVFSEADQQYILSSAQSAFLGTIVLSQFWHIFHVKTRVNSIFEHGVFNNRLMNAGVLVELGLVIAIVYVPFMNSAFGTAYGGQ-TELWTMNLVSAVYLTGFNEFRKYMLRKDLTSTFSHWMGW 1129          

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|857971766|emb|CEP02260.1| (hypothetical protein PBRA_002525 [Plasmodiophora brassicae])

HSP 1 Score: 682.174 bits (1759), Expect = 0.000e+0
Identity = 438/1199 (36.53%), Postives = 654/1199 (54.55%), Query Frame = 0

Query:   37 SFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPE-----------------GLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLDCRTRTTEYMTREGNVDKWDSFKELVTAGALCNMCKYEADPNAPAVAAAGSLDLSQATNRPSIATMNNYTLSKRPAAVAGPLGNHQMTYQGTLARRSMQQQKGEAAKSTILNPGFFDRGAAALEASTGAGAGAGADTVRGMANLPNIVRQLSSVTGQATVGRGSQATFGRNSQATFGRNSQALQGAGTLNRLSVNALGRNTFLGGLPLVEAPPR---IIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPP---TQADQRRVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASV---DDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTG------------------DGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDL-----FQFGVTDDFALRDGRVLSLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAF-SAYGRQPIQFFLFFLVSGAILVGWGELRKFLIRVYPTSCFANAFAF 1185
            S +EL       +N +    S+GL   +A++RL  +G N +TP P  P +       LDP ++LL+   VL      T   +  +  +LY+ ++L  ++  T+ LSF+QEGQ+   M  F+ MM ++A VRRGG +  + A DLV GD+V++ +G RVPAD+ ++ +  L+++ SS TGE  PV+MC     +     +++A + + V+ G G GIVVRTGD   IG IASL  Q    +STL   V  FV LI   AI+ A   + V +I+       G  +  +  ++ FIV+L+ANVPE                 GLPA V+ CL V A+R+A + V VKRL+ ++TLG+ T + +DKTGTLTQN M V+ L+ +   +  + ++   ++ +  S ++L     L ++C +  DP    +  +   + + AT+   I         + PA         Q   +  + +R+   + G+A       P     G+  L      G    +D V    + P ++  +       T+ R      G+       R       A    R   +AL + T +         PR   ++ GN SD A+F F   + D+   R+E   ++++PF+S  K+M TIH  P    T    + ++I+KGA E +L  C   + KG  +P+        E    + A +GERVL  A   LP      S    +D+  +++     +  T +  ++L DPPK GV DAVL+C+ AGIRV+MITGDHP TAEAIA+ VNII+ Y  R++IA    +  +   E   GAAV+T  ++E   ++ W+ LL   E+VFAR SP +KL+I   L+A+ E+ AMTG                  DGVNDSPAL+ ADIGIAMG+ G +VA+ AA+VVL+DDDFC+IVNGIEEGR++FDNL K IAYTLTHLV E+ A+ I +   +P G++S MILSIDL TEV P++S ++E AE  +M RPPR+ KVDRLV   L  Y Y+ AG+  A + + AY +V+ S+G+   +L     FQ G      L    V S  +Q  I++SA +AF  T++LSQ  ++F  KTR   + EHGVFNN +MN GV+VE+G++IAIV++P +N AF +AYG Q  + +   LVS   L G+ E RK+++R   TS F++   +
Sbjct:   28 SLDELVKRTRTSVNTSDLDVSSGLKPEEAEERLRNDGKNCMTPKPPVPLWKRFGKHLLDPMLVLLDFAGVLSI---ATALSTPGDTTDLYLGILLFILVVCTAGLSFFQEGQAESAMQGFQKMMPQNACVRRGGVQTTINAVDLVRGDIVILSTGDRVPADVALLTARGLRIDMSSMTGESLPVKMC--SILEPPAAGESIALSGSQVMAGNGTGIVVRTGDRCTIGQIASLANQASTTESTLRRNVEHFVKLIAALAIVMAAAMFIVTIIQQ------GTKNILQRIVSSFIVVLVANVPEVTAPSHFQGRRLIAATKGLPAAVSGCLAVAARRMAAKNVLVKRLEVLDTLGAVTTICTDKTGTLTQNHMAVACLFYEGEVQPADSLSHPRHLKELKSAEQL---HGLFDIC-FSNDPLHCTIVFSALCNQATATDFVQI--------EEAPAETKLTAARTQ-DVRIDMVKRAGSGRTGKA-------PDAERHGSLVL------GMAESSDNVARFKSSPVLIDDV-------TIKRVRTMPIGKQDFTNINRAE-----APVPTRGKADALRKQTRV---------PRERDVVAGNASDVAMFCFAERYVDVMELRQEIRTIFMVPFSSERKWMATIHGVPAADRTNRTSKHLLIVKGALERILPKCKLVLKKGAVVPLTPDAIAEVESACGDFARRGERVLVMAMATLPAQPDSFSYTFEEDEAGNIKSNFTIDNLTLISCLALHDPPKVGVADAVLKCQRAGIRVIMITGDHPATAEAIARHVNIIKHYHTREEIAAMNLDGQSGDVE-LAGAAVVTGAEMESMTDQQWDELLSMHEIVFARTSPMNKLEIVTRLQAKGEICAMTGGTHYVGIRLHSPADSDFLDGVNDSPALRRADIGIAMGLRGHDVARDAADVVLVDDDFCTIVNGIEEGRLIFDNLRKIIAYTLTHLVAEVAAIAITLLAGLPPGLNSFMILSIDLGTEVCPSLSLSYETAEDAIMQRPPRDDKVDRLVSIPLFLYMYVWAGIPVAMLSIGAYLLVFQSHGIPLSELVHTSYFQPGCGP--LLIGSTVFSEADQQYILSSAQSAFLGTIVLSQFWHIFHVKTRVNSIFEHGVFNNRLMNAGVLVELGLVIAIVYVPFMNSAFGTAYGGQ-TELWTMNLVSAVYLTGFNEFRKYMLRKDLTSTFSHWMGW 1164          

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|470266027|ref|XP_004361660.1| (P-type ATPase [Dictyostelium fasciculatum] >gi|328875445|gb|EGG23809.1| P-type ATPase [Dictyostelium fasciculatum])

HSP 1 Score: 461.84 bits (1187), Expect = 2.788e-138
Identity = 260/621 (41.87%), Postives = 371/621 (59.74%), Query Frame = 0

Query:  555 ATVGRGSQATFGRNSQATFGRNSQALQGAGTLNRLSVNALGRNTFLGGLPLVEAPPRIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPPTQADQRRVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDL-------FQFGVTDDFALRDGRVLSLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRK 1168
            ATVGR     F +NS       ++ L+     NR   + L     L G    +   R+I+G+ S+ A+   C+    I   R   P+++ IPFNS  K+  +IH+     A+  RV+++KGAPEI++  C++ M  G+E+ ID  +   F + Y+ +   GER LGFAR  L  S++    D+ Y       P  + TF+GL SL+DPP+  VP AV +CK AGI+V+M+TGDHP+TA+AIAKKV II   +  +DIA++ G     + +  + A V+    +    ++DW+ +L K E+VFAR SPQ K  I  + + R EVVA+TGDGVNDSPALK ADIG+AMGI+G++VAK  A+++L+DD+F SIV GIEEGR++FDNL K+IAYTL+H VPE+   L+NI   IP  ++S +IL IDL TE+ PAIS A+E  E ++M R PR   VD LV  +LLSY+YLQAG IEA      +F+V  S G  P DL       FQ G   DF   DG   +   Q   +  A TA++ TL++ Q  N+   KTR VP+  HG+  N+ +N+G+++E GIL+ I++ P +++        P  ++ F L     L  W ELRK
Sbjct:  664 ATVGR---EMFNKNSTTC----AELLKICANCNRADYDKL-----LEGNMERQIDQRLILGDASESALVRLCDKNDPIVEVRAANPKVFEIPFNSANKWQLSIHKST-NPANTSRVLMMKGAPEIIISKCSKIMIDGKEVEIDDKWRNDFTQAYEALGSMGERCLGFARIILDQSEYGPMHDNLYDAQTLNFPTSQLTFVGLCSLLDPPRENVPFAVHQCKTAGIKVIMVTGDHPITAKAIAKKVGIISSPTA-EDIAEQKGIPLDQVDDSEVKAVVLHGAQIRELTDKDWDRVLAKSEIVFARTSPQQKQQIVENAQKRKEVVAVTGDGVNDSPALKKADIGVAMGIVGSDVAKETADIILLDDNFASIVAGIEEGRIIFDNLKKSIAYTLSHAVPEIAPFLLNIIAGIPLAITSFLILCIDLGTELAPAISLAYETGEKDIMTRKPRQLGVDHLVTTNLLSYSYLQAGPIEAIASFLNFFLVMASYGFRPQDLPYTAKDYFQEGAP-DFVTSDGVARNAHYQLTALEEAQTAYFMTLVMCQFFNLITNKTRVVPVWRHGM-KNMFVNFGLVIEAGILVFIIYTPFVHNIIEN-ASVPGIYWAFPLPIIFCLFAWNELRK 1267          

HSP 2 Score: 315.464 bits (807), Expect = 7.886e-86
Identity = 169/371 (45.55%), Postives = 234/371 (63.07%), Query Frame = 0

Query:   28 EVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLD 398
            E +  EH +S E+L       +N++ P +S GL   DA   L+  G N L PP   PW+  L   F + FMILLEI   LCFI +  +       VNLY+  VL  ++  T +L+F QE QS+ +MN+FKN++ +S RV R G E  +P ED+V+GD+VV+ +G +VP D+R+I   S+KV+NSS TGE E     +  TDD  L S NLAF   +V++G   G+V+RTG NT+IG IA L   T+  ++TL+ E   FVH I    I   I+F+ +      GF +G    P  + INV  +I +ANVPEGLP+T+T+CLTV A+RL+ R V+ K+L+ +ETLGS T++ASDKTGTLTQN+MTVSH+W D
Sbjct:  295 EYDVDEHEFSLEQLAIKFGTNINLSDPSQSQGLRQEDAATLLLRYGKNVLKPPKQTPWFVQLFLCFTNFFMILLEIAGALCFIAYALD---TSQRVNLYLGCVLWAIVIFTCMLTFAQERQSSNIMNSFKNLLPQSCRVVRDGSETKIPVEDVVIGDVVVVSAGDKVPGDIRVITCSSMKVDNSSLTGESEAQSCTVTCTDDNPLESHNLAFYGTLVMDGSARGVVIRTGTNTLIGKIADLASNTETTETTLQIETKRFVHFIAILGISMGIIFFVI------GFGVG--LQPIPNLINVLGLI-VANVPEGLPSTITACLTVTARRLSKRNVYAKKLESIETLGSITLIASDKTGTLTQNRMTVSHMWYD 653          

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|1160565393|ref|XP_020428905.1| (P-type ATPase [Polysphondylium pallidum PN500] >gi|281202571|gb|EFA76773.1| P-type ATPase [Polysphondylium pallidum PN500])

HSP 1 Score: 459.529 bits (1181), Expect = 1.327e-137
Identity = 271/690 (39.28%), Postives = 397/690 (57.54%), Query Frame = 0

Query:  490 LARRSMQQQKGEAAKSTILNPGFFDRGAAALEASTGAGA-GAGADTVRGMANLPNIVRQLSSVTGQATVGRGSQATFGRNSQATFGRNSQALQGAGTLNRLSVNALGRNTFLGGLPLVEAP--PRIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPPTQADQR-RVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTP-------LDLFQFGVTDDFALRDGRVLSLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRK 1168
            L+RR++  +K E+ ++          G+  L AS   G       TV  M     IV+   ++    T+GR   A F  NS       S  L+     NR   + L           +E P   R+I+G+ S+ A+   C+  ++IE TR   P+++ IPFNS  K+  +IHR      D+  RV+++KGAPEI+   C++ M  G+++ ID      F++ Y+E+   GER LGFA+  L   ++    D+ Y   +   P  + TF+GL SL+DPP+  VP AV +CK AGI+V+M+TGDHP+TA+AIAKKV II   +  +DIA   G    ++ E  + A V+    +    E DW+ +L K E+VFAR SPQ K  I    + R EVVA+TGDGVNDSPALK ADIG+AMGI+G++VAK  A+++L+DD+F SIV G+EEGR++FDNL K+IAYTLTH  PE++  ++NI   IP  ++S ++L IDL TE+ PAIS A+E+ E ++M R PR   VD LV  +LLSY+YLQAG +EA   L ++F+V  S G  P        D FQ G    F + DG+  +   Q   +  A TA++ TL++ Q  N+   KTR VP+  HG+  N+ +N G+++E GI   I++ P ++   S+     I ++ + L    +L  W E RK
Sbjct:  589 LSRRNVYAKKLESIETL---------GSITLIASDKTGTLTQNRMTVSHMWYDNTIVK---AINDGMTIGR---AMFDENSPTC----SDLLRIGACCNRSDFDKLAEGN-------MEKPIDQRLILGDASESALIRLCHKLEEIEITRANNPKIFEIPFNSTNKWQLSIHRRSSDPNDKSTRVLMMKGAPEIIFAKCSKIMMNGKDIDIDDKMRYDFQQAYEELGSMGERCLGFAQIVLDEKEYGPMHDNLYDAQQLNFPTSQLTFVGLCSLLDPPRENVPFAVHQCKTAGIKVIMVTGDHPITAKAIAKKVGIISSPTA-EDIAAAKGIPLEEVDESEVKAVVLHGSQIRELTETDWDRVLSKSEIVFARTSPQQKSQIVEQAQRRKEVVAVTGDGVNDSPALKKADIGVAMGIVGSDVAKETADIILLDDNFASIVAGVEEGRIIFDNLKKSIAYTLTHAFPEVIPFVLNIILGIPLAITSFLVLCIDLGTELAPAISLAYEKGEKDIMTRKPRILGVDHLVTTNLLSYSYLQAGPMEAITSLMSFFLVMASYGYPPSSLLYSAKDYFQEGA-QPFEV-DGKFYTASHQVAALEEAQTAYFMTLVMCQFFNLITNKTRVVPLWRHGM-ANMYVNIGLIIEAGICCFIIYTPFVHTIISSANVSGI-YWAYPLPMVFLLFAWNETRK 1247          

HSP 2 Score: 314.309 bits (804), Expect = 1.502e-85
Identity = 165/371 (44.47%), Postives = 237/371 (63.88%), Query Frame = 0

Query:   28 EVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLD 398
            E + V+H  + E+L       ++   P +S GLT+  AK  L   GPN L PP   PWY  L   F++ FMILLE+  VLCFI F  +    D  VNLY+  VL  ++  T +L+F QE QS+ +MN+FK ++ ++ RV R G E  +P  D+V+GD+VV+ +G ++PAD+R+I  + +KV+NSS TGE EP    +  TD+  L + NLAF   +V++G   G+V+RTG NT+IG IA +   T+  ++TL+ E   FV  I+   I   ++F+ V      GF +G    P  + INV  +I +ANVPEGLP+T+T+CLTV A+RL+ R V+ K+L+ +ETLGS T++ASDKTGTLTQN+MTVSH+W D
Sbjct:  274 EYDVVDHESTLEQLSEKFGTNIHFTDPSQSQGLTSESAKLLLERYGPNILKPPKESPWYVKLALCFVNFFMILLEVAGVLCFIAFALD---TDQRVNLYLGCVLWAIVIFTCLLTFAQEWQSSNIMNSFKKLLPQATRVVRDGMETRIPVADIVIGDVVVVSAGDKIPADIRVITCNGMKVDNSSLTGESEPQSCTVTCTDENPLETHNLAFYGTLVMDGSARGVVIRTGGNTLIGKIADMASNTQTAETTLQIETKRFVRFISALGITMGLIFFIV------GFGVG--LKPIPNLINVLGLI-VANVPEGLPSTITACLTVTARRLSRRNVYAKKLESIETLGSITLIASDKTGTLTQNRMTVSHMWYD 632          

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|330844080|ref|XP_003293965.1| (hypothetical protein DICPUDRAFT_42780 [Dictyostelium purpureum] >gi|325075643|gb|EGC29505.1| hypothetical protein DICPUDRAFT_42780, partial [Dictyostelium purpureum])

HSP 1 Score: 451.44 bits (1160), Expect = 3.224e-137
Identity = 241/561 (42.96%), Postives = 353/561 (62.92%), Query Frame = 0

Query:  611 RIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPPTQADQRRVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDLFQFGVTDDFALRDGRVL---SLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRK 1168
            R+I+G+ S+ A+   C+  +DIE TR +  +++ IPFNS  K+  +IH+     +  R ++ +KGAPEI+ + C++ M  G+E+PID   +  F   Y+ +   GER LGFA+  L L+D     +D Y       P    TF+GL SL+DPP+  VP AV +CK AGI+V+M+TGDHP+TA+AIAKKV II   ++ +DIAQE G   +++ +   GA V+    L  + E DW+ +L K E+VFAR SPQ K  I  + + R EVVA+TGDGVNDSPALK ADIG+AMG++G++VAK  A+++L+DD+F SIV GIEEGR++FDNL K+IAYTL+H +PE+   L+NI   IP  ++S +IL IDL TE+ PAIS A+E+ E ++M R PR    D LV  +LLSY+YLQAG IEA +    +F+V  + G  P  L   G T D+ +     L   +  +Q   +  A TA++ TL++ Q  N+   KTR VP+  HG+  N+ +N G+++E GI   +++ P ++    +    P +F+ + L     L  W E+RK
Sbjct:  416 RLILGDASESALVRLCHKIEDIEITRAKNSKVFEIPFNSTNKWQLSIHKLENDNSSSR-ILYMKGAPEIIFEKCSKIMQNGKEIPIDEKINADFITAYEALGSMGERCLGFAQ--LLLTDENTVPNDLYDAQILNFPMTGLTFVGLCSLLDPPRENVPFAVHQCKTAGIKVIMVTGDHPITAKAIAKKVGIISSPTV-EDIAQERGVPVSEVDDSEAGAIVLHGAQLREFTEADWDRVLSKGEIVFARTSPQQKSQIVENAQRRKEVVAVTGDGVNDSPALKKADIGVAMGVVGSDVAKETADIILLDDNFASIVAGIEEGRIIFDNLKKSIAYTLSHAIPEIAPFLLNIVSGIPLAITSFLILCIDLGTEMAPAISLAYEKGEKDIMTRKPRVLGKDHLVTTNLLSYSYLQAGPIEAIVSFLNFFLVMAAEGFPPHIL--PGTTQDYFIETALPLNGKNAHQQITALRKAQTAYFMTLVMCQFFNLITNKTRVVPLWRHGMM-NMYVNIGLIIEAGICCFVIYTPFIHTIIESE-VFPGKFWAYPLPMVFCLFAWNEIRK 968          

HSP 2 Score: 328.946 bits (842), Expect = 2.405e-92
Identity = 173/414 (41.79%), Postives = 251/414 (60.63%), Query Frame = 0

Query:   28 EVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLDCRTRTTEYMTREGNVDK--WD----SFKELVTAGALCNMCKYE 435
            E +  EH  + E+L       +N + P +S GLT++ A + L   G N L PP   PWY  L   F + FMILLE+   LCF+ +G ++      VNLY+A VL  ++  T +L+F QE Q+  +M +FKN++ +S RV R G E  +  ED+V+GD+VV+ +G +VP D+RII  + +KV+NSS TGE E     +  TDD  L S NLAF   +V++G   G+V+RTG NT+IG IA +   T+  ++TL+ E   FVH IT   I   ++F+ +      GF +G    P  + INV  +I +ANVPEGLP+T+T+CLTV A+RL+ R V+ K+L+ +ETLGS T++ASDKTGTLTQN+MTVSH+W D        +   G +D+  +D    +   L+  GA CN   ++
Sbjct:    3 EYDVNEHELTLEQLSDKFTTDINFSDPSQSGGLTSSLASELLSRYGKNVLKPPKEIPWYVQLAKCFTNFFMILLEVAGALCFLAYGLDRSI---RVNLYLACVLYAIVIFTCLLTFSQERQTGNIMKSFKNLLPQSCRVIRDGSETKIKVEDIVIGDIVVVSAGDKVPGDIRIITCNGMKVDNSSLTGESEAQSCTVTCTDDNPLESHNLAFYGTLVMDGSARGVVIRTGTNTLIGKIADMASNTEQTETTLQIETKRFVHFITVLGITMGLLFFII------GFCVG--IKPIPNLINVLGLI-VANVPEGLPSTITACLTVTARRLSKRNVYSKKLESIETLGSITLIASDKTGTLTQNRMTVSHMWYD--NTIFRAIENGGTIDRTLFDLGTPTCSNLIRVGACCNRADFD 402          

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|1575684|gb|AAB09569.1| (IonA, partial [Dictyostelium discoideum])

HSP 1 Score: 451.44 bits (1160), Expect = 4.154e-137
Identity = 244/582 (41.92%), Postives = 352/582 (60.48%), Query Frame = 0

Query:  611 RIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPPTQADQR----RVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDLFQFGVTDDFALRD-GRVLSLVE--QTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRKFLIRVYPTSCFANAFAF 1185
            R+I+G+ S+ A+   C+  +DIE TR + P+++ IPFNS  K+  +IH+ P  Q        R++ +KGAPEI+ + C++ M  G+EL ID      F + Y+ +   GER LGFA+  L L +     +D Y       P    TF+GL SL+DPP+  VP AV +CK AGI+V+M+TGDHP+TA+AIAKKV II   +  +DIA E G   + + ++ + A V+    +    + DW+ +L K E+VFAR SPQ K  I  + + R EVVA+TGDGVNDSPALK ADIG+AMGI+G++VAK  A+++L+DD+F SIV GIEEGR++FDNL K+IAYTL+H VPE+   L+NI   IP  ++S +IL IDL TE+ PAIS A+E  E ++M R PR    D LV  +LLSY+YLQAG IEA I    +F+V   +G  P  L   G T+D+     G+ L      Q   +  A TA++ TL+  Q  N+   +TR VP+  H + +N  +N G+++E GI   +V+ P ++    +    P  F+ + L     L  W E+RK +    P    +++  F
Sbjct:  416 RLILGDASESAILRLCHTIEDIEITRAKNPKVFEIPFNSTNKWQLSIHKLPNDQPQSNTSTARILYMKGAPEIIFEKCSKLMINGKELEIDEKIKNDFIQAYEALGSMGERCLGFAQ--LLLDNENTVPNDMYDAQTLNFPMTGLTFVGLCSLLDPPRENVPFAVHQCKTAGIKVIMVTGDHPITAKAIAKKVGIISSPTA-EDIALERGIPLSQVDDNEVKAVVLHGGQIRELTDADWDRVLSKSEIVFARTSPQQKSQIVENAQKRKEVVAVTGDGVNDSPALKKADIGVAMGIVGSDVAKETADIILLDDNFASIVAGIEEGRIIFDNLKKSIAYTLSHAVPEVAPFLLNIISGIPLAITSFLILCIDLGTEMAPAISLAYETGEKDIMSRKPRVLGKDHLVTTNLLSYSYLQAGPIEAIISFLNFFLVLAHHGFPPHSL--PGTTNDYFFEGCGKYLGFESDYQVEALRQAQTAYFMTLVTCQFFNLITNRTRVVPLWSHKILSNWYINIGLVIEAGICAFVVYTPFVHTIIES-ASVPGLFWAYPLPMIFCLFSWNEIRKRIAYDRPDWKISSSLGF 991          

HSP 2 Score: 330.102 bits (845), Expect = 1.173e-92
Identity = 165/371 (44.47%), Postives = 240/371 (64.69%), Query Frame = 0

Query:   28 EVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLD 398
            E +  EH  + E++    +  +N   P  S+GLT + A + L+ NG N L PP   PWY  L   F + FMILLE+   LCF+ +G ++   +  VNLY+A VL  ++  T +L+F QE Q+  +M +FKN++ +S RV R G E  V  ED+V+GD+V+ ++G +VPAD+RII  + +KV+NSS TGE +     +  TDD  L + NLAF   +V++G   G+V+RTG NT+IG IA L   T+  ++TL+ E+  FVH IT   I   ++F+ +      GF +G   D  ++ INV  +I +ANVPEGLP+T+T+CLTV A+RL+ R V+ K+L+ +ETLGS T++ASDKTGTLTQN+MTVSH+W D
Sbjct:    3 EYDVNEHELTLEQISEKFSTNINSNDPSLSSGLTQSLANELLIRNGKNILKPPKEVPWYVQLGKCFTNFFMILLEVAGALCFLAYGLDR---NQRVNLYLACVLYAIVIFTCLLTFSQERQTGNIMKSFKNLLPQSCRVIRDGSETKVKVEDIVVGDIVMCQAGDKVPADIRIITCNGMKVDNSSLTGESDAQSCTVTCTDDNPLETHNLAFCGTLVMDGSARGVVIRTGSNTLIGKIADLASNTEQTETTLQIEIKRFVHFITALGITMGLIFFII------GFAVG--IDSIQNLINVLGLI-VANVPEGLPSTITACLTVTARRLSRRNVYSKKLESIETLGSITLIASDKTGTLTQNRMTVSHMWYD 361          

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|66818235|ref|XP_642777.1| (P-type ATPase [Dictyostelium discoideum AX4] >gi|60470848|gb|EAL68820.1| P-type ATPase [Dictyostelium discoideum AX4])

HSP 1 Score: 452.595 bits (1163), Expect = 3.027e-135
Identity = 244/582 (41.92%), Postives = 352/582 (60.48%), Query Frame = 0

Query:  611 RIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPPTQADQR----RVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDLFQFGVTDDFALRD-GRVLSLVE--QTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRKFLIRVYPTSCFANAFAF 1185
            R+I+G+ S+ A+   C+  +DIE TR + P+++ IPFNS  K+  +IH+ P  Q        R++ +KGAPEI+ + C++ M  G+EL ID      F + Y+ +   GER LGFA+  L L +     +D Y       P    TF+GL SL+DPP+  VP AV +CK AGI+V+M+TGDHP+TA+AIAKKV II   +  +DIA E G   + + ++ + A V+    +    + DW+ +L K E+VFAR SPQ K  I  + + R EVVA+TGDGVNDSPALK ADIG+AMGI+G++VAK  A+++L+DD+F SIV GIEEGR++FDNL K+IAYTL+H VPE+   L+NI   IP  ++S +IL IDL TE+ PAIS A+E  E ++M R PR    D LV  +LLSY+YLQAG IEA I    +F+V   +G  P  L   G T+D+     G+ L      Q   +  A TA++ TL+  Q  N+   +TR VP+  H + +N  +N G+++E GI   +V+ P ++    +    P  F+ + L     L  W E+RK +    P    +++  F
Sbjct:  657 RLILGDASESAILRLCHTIEDIEITRAKNPKVFEIPFNSTNKWQLSIHKLPNDQPQSNTSTARILYMKGAPEIIFEKCSKLMINGKELEIDEKIKNDFIQAYEALGSMGERCLGFAQ--LLLDNENTVPNDMYDAQTLNFPMTGLTFVGLCSLLDPPRENVPFAVHQCKTAGIKVIMVTGDHPITAKAIAKKVGIISSPTA-EDIALERGIPLSQVDDNEVKAVVLHGGQIRELTDADWDRVLSKSEIVFARTSPQQKSQIVENAQKRKEVVAVTGDGVNDSPALKKADIGVAMGIVGSDVAKETADIILLDDNFASIVAGIEEGRIIFDNLKKSIAYTLSHAVPEVAPFLLNIISGIPLAITSFLILCIDLGTEMAPAISLAYETGEKDIMSRKPRVLGKDHLVTTNLLSYSYLQAGPIEAIISFLNFFLVLAHHGFPPHSL--PGTTNDYFFEGCGKYLGFESDYQVEALRQAQTAYFMTLVTCQFFNLITNRTRVVPLWSHKILSNWYINIGLVIEAGICAFVVYTPFVHTIIES-ASVPGLFWAYPLPMIFCLFSWNEIRKRIAYDRPDWKISSSLGF 1232          

HSP 2 Score: 334.724 bits (857), Expect = 9.585e-93
Identity = 172/398 (43.22%), Postives = 248/398 (62.31%), Query Frame = 0

Query:    1 MATEVELGKSKKSAASNGKKNGADEKPEVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLD 398
            M    EL K  KSA          +  E +  EH  + E++    +  +N   P  S+GLT + A + L+ NG N L PP   PWY  L   F + FMILLE+   LCF+ +G ++   +  VNLY+A VL  ++  T +L+F QE Q+  +M +FKN++ +S RV R G E  V  ED+V+GD+V+ ++G +VPAD+RII  + +KV+NSS TGE +     +  TDD  L + NLAF   +V++G   G+V+RTG NT+IG IA L   T+  ++TL+ E+  FVH IT   I   ++F+ +      GF +G   D  ++ INV  +I +ANVPEGLP+T+T+CLTV A+RL+ R V+ K+L+ +ETLGS T++ASDKTGTLTQN+MTVSH+W D
Sbjct:  224 MGQTAELSKKDKSATKQ-------KASEYDVNEHELTLEQISEKFSTNINSNDPSLSSGLTQSLANELLIRNGKNILKPPKEVPWYVQLGKCFTNFFMILLEVAGALCFLAYGLDR---NQRVNLYLACVLYAIVIFTCLLTFSQERQTGNIMKSFKNLLPQSCRVIRDGSETKVKVEDIVVGDIVMCQAGDKVPADIRIITCNGMKVDNSSLTGESDAQSCTVTCTDDNPLETHNLAFCGTLVMDGSARGVVIRTGSNTLIGKIADLASNTEQTETTLQIEIKRFVHFITALGITMGLIFFII------GFAVG--IDSIQNLINVLGLI-VANVPEGLPSTITACLTVTARRLSRRNVYSKKLESIETLGSITLIASDKTGTLTQNRMTVSHMWYD 602          

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|1013946394|gb|KYR00069.1| (P-type ATPase [Dictyostelium lacteum])

HSP 1 Score: 451.44 bits (1160), Expect = 3.341e-135
Identity = 269/700 (38.43%), Postives = 400/700 (57.14%), Query Frame = 0

Query:  490 LARRSMQQQKGEAAKSTILNPGFFDRGAAALEASTGAGA-GAGADTVRGMANLPNIVRQLSSVTGQATVGRGSQATFGRNSQATFGRNSQALQGAGTLNRLSVNALGRNTFLGGLPLVEAP--PRIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRP---PPTQADQRRVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDL-------FQFGVTDDFALRDGRVLSLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIVFIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRKFLIRVYPT 1176
            L++R++  +K E+ ++          G+  L AS   G       TV  M    +IVR + S     T+GR     F +NS       SQ ++     NR   + L           +E P   R+I+G+ S+ A+   C+  +DI+ TR   P+++ IPFNS  K+  +IH+       Q    R++++KGAPEI+ + C++ M +G+E+ +D      F   Y+++   GER LGFA+  +PLS      +D Y       P    TF+GL SL+DPP+  VP AV +CK AGI+V+M+TGDHP+TA+AIAKKV II   +  +DIAQ+ G     + +  + + V+    +  + +EDW+ +L K E+VFAR SPQ K  I  + + R EVVA+TGDGVNDSPALK ADIG+AMGI+G++VAK  A+++L+DD+F SIV G+EEGR++FDNL K+IAYTL+H VPE+   L+NI   +P  ++S +IL IDL TE+ PAIS A+E  E+++M R PR    D LV  +LL+Y+Y Q G +   IC   +F+V  S G  P  L       FQ    D   + DG+      Q   ++ A TA++ TL++ QV N+   KTR +P+ +HG+  N+ +N G+++E GIL  +++ P ++          I F+ F L   A +  W ELRK L   Y T
Sbjct:  524 LSKRNVYSKKLESIETL---------GSITLIASDKTGTLTQNRMTVSHMWYDNHIVRAIPS---SNTIGR---ELFDQNSPTC----SQLIKIGACCNRSDFDKLLEGN-------MEKPVEQRLILGDASESALVRLCHKIEDIDITRSNNPKVFEIPFNSTNKWQLSIHKQLLVTEGQDPNDRILLMKGAPEIIFEKCSKIMIQGKEVELDDTIRADFVRAYEDLGSMGERCLGFAQ--IPLSKENTVPNDLYDAQTLNFPMTGLTFVGLCSLLDPPRENVPAAVQQCKTAGIKVIMVTGDHPITAKAIAKKVGIITSPTA-EDIAQQKGIPIDKVDQSEVKSVVVHGAQIREFTKEDWDHILSKSEIVFARTSPQQKSQIVENAQNRGEVVAVTGDGVNDSPALKKADIGVAMGIVGSDVAKETADIILLDDNFASIVAGVEEGRIIFDNLKKSIAYTLSHAVPEIFPFLLNIIAGLPLALTSFLILCIDLGTELAPAISLAYEVGENDIMTRKPRKLGTDHLVTGNLLTYSYFQIGPMTTIICFLNFFLVMLSWGFPPSSLPGTASTYFQPDCPD--LIVDGKAYDSDYQMNAISEAQTAYFMTLVMCQVFNLLTNKTRVIPLWKHGM-KNMAVNIGIVIEAGILCFVIYTPFVHTIIGNNSVTGI-FWAFPLPMIACIFVWNELRKRLAFDYDT 1190          

HSP 2 Score: 303.523 bits (776), Expect = 3.987e-82
Identity = 170/412 (41.26%), Postives = 249/412 (60.44%), Query Frame = 0

Query:   28 EVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLDCR----TRTTEYMTREGNVDKWDSFKELVTAGALCNMCKYE 435
            E +  EH  +  E+       +N   P +SAGLT + A + L   G N L PP   PWY  L   F++ FMILLEI   LCF+ +G +       VNLY+  VL  ++  T +L+F+QE QS+ +MN+FKN++ +  RV R G E  +  ED+V+GD+VV+ +G ++P D+R+I  + +KV+NSS TGE EP    +  TDD  L + NLAF   +V++G   G+V+RTG NT+IG IA +   T   ++TL+ E   FV +I+   I   ++F+ +      GF IG    P  + INV  +I +ANVPEGLP+T+T+CLTV A+RL+ R V+ K+L+ +ETLGS T++ASDKTGTLTQN+MTVSH+W D        ++  + RE       +  +L+  GA CN   ++
Sbjct:  209 EYDVDEHEMTLMEIATKYTTDINEKDPSQSAGLTQSLAAELLSRYGKNVLKPPKETPWYVQLGRCFINFFMILLEIAGALCFLAYGLD---TSQRVNLYLGCVLFAIVIFTCLLTFFQERQSSNIMNSFKNLLPQFCRVIRDGGETKIKVEDVVVGDIVVVAAGDKIPGDIRVITCNGMKVDNSSLTGESEPQSCTVTCTDDNPLETHNLAFYGTLVMDGSARGVVIRTGGNTLIGKIADMASTTANVETTLQIETKRFVKIISALGISMGLLFFII------GFAIG--FKPIPNLINVLGLI-VANVPEGLPSTITACLTVTARRLSKRNVYSKKLESIETLGSITLIASDKTGTLTQNRMTVSHMWYDNHIVRAIPSSNTIGRELFDQNSPTCSQLIKIGACCNRSDFD 608          

BLAST of EWM23961.1 vs. NCBI_GenBank
Match: gi|831779070|ref|XP_012755606.1| (hypothetical protein SAMD00019534_046620 [Acytostelium subglobosum LB1] >gi|735856238|dbj|GAM21487.1| hypothetical protein SAMD00019534_046620 [Acytostelium subglobosum LB1])

HSP 1 Score: 445.277 bits (1144), Expect = 3.051e-132
Identity = 238/568 (41.90%), Postives = 350/568 (61.62%), Query Frame = 0

Query:  611 RIIVGNPSDKAVFTFCNLFKDIETTREEYPQLYIIPFNSRLKYMKTIHRPPPTQAD-QRRVVILKGAPEIVLKSCTRYMSKGEELPIDAGFSKRFEEVYKEIAGQGERVLGFARKHLPLSDFPASVDDKYKDMEDKVPHEEFTFLGLISLIDPPKNGVPDAVLRCKNAGIRVMMITGDHPLTAEAIAKKVNIIRDYSMRDDIAQETGESPADITEDRIGAAVITCKDLEHYKEEDWNVLLDKPELVFARASPQDKLDICRHLRARDEVVAMTGDGVNDSPALKNADIGIAMGIMGTEVAKGAANVVLMDDDFCSIVNGIEEGRVLFDNLTKTIAYTLTHLVPELMAVLINICFTIPAGMSSLMILSIDLLTEVPPAISYAWEQAESNVMDRPPRNRKVDRLVRPSLLSYAYLQAGMIEAGICLFAYFMVYGSNGVTPLDLFQFGVT----DDFALRDGRVLSLVEQTRIVNSAMTAFYATLILSQVVNVFVCKTRFVPMLEHGVFNNIMMNYGVMVEVGILIAIV---FIPGLNDAFSAYGRQPIQFFLFFLVSGAILVGWGELRKFL 1170
            R+I+G+ S+ A+    +  ++IE TR   P+++ IPFNS  K+  +IHR      D   RV+++KGAPEI+   CT+ M  G+++PI    S+ F+  Y+++   GER LGFA+  L  S +    D+ Y       P  + TF+GL SL+DPP+  VP AV +CK AGI+V+M+TGDHP+TA+AIAKKV II   +  +DIA       +++ +  + + V+    +    + DW+ +L K E+VFAR SPQ K  I    + R EVVA+TGDGVNDSPALK ADIG+AMGI+G++VAK  A+++L+DD+F SIV G+EEGR++FDNL K+IAYTLTH  PE+   ++ +   IP  ++S ++L IDL TE+ PAIS A+E+ ES++M R PR   VD LV  +LLSY+YLQAG IEA  CL  +F+V  S G  P  L          D+   +     S   Q   +  A TA++ TL++ Q  N+   KTR +P+  HG+ +N+ +N G+++E GIL  I+   F+PG+  + +  G     ++ F L    +L  + ELRK +
Sbjct:  695 RLILGDASESAIVRLVHKMENIEVTRANNPKIFEIPFNSTNKWQLSIHRRSSDPNDTSTRVLMMKGAPEIIFSKCTKIMMGGKDVPITEKISEDFQRAYEDLGSMGERCLGFAQIVLDESIYGPMHDNLYDAQTLNFPISQLTFVGLCSLLDPPRENVPHAVHQCKTAGIKVIMVTGDHPITAKAIAKKVGIISTPTA-EDIAAAKCIPLSEVDDSEVKSVVLHGNQIRELTDADWDRVLSKSEIVFARTSPQQKSQIVEQAQKRKEVVAVTGDGVNDSPALKKADIGVAMGIVGSDVAKETADIILLDDNFASIVAGVEEGRIIFDNLKKSIAYTLTHAFPEVAPFVLYLVLGIPLALTSFLVLCIDLGTELAPAISLAYEKGESDIMTRKPRVLGVDHLVTKNLLSYSYLQAGPIEAIACLMNFFLVLNSYGFPPYSLIGSATNHFSDDELVCKTLFGHSCSYQMEALKEAQTAYFMTLVMCQFFNLITNKTRVMPIWRHGM-SNMYVNGGLIIEAGILCFIIYAPFVPGIIQSKAVSGI----YWAFPLPMIFLLFAYNELRKHV 1256          

HSP 2 Score: 324.324 bits (830), Expect = 6.284e-89
Identity = 175/425 (41.18%), Postives = 256/425 (60.24%), Query Frame = 0

Query:   15 ASNGKKNGADEKPEVEYVEHAWSFEELEGNLAIKLNVAQPGKSAGLTAADAKKRLVENGPNALTPPPVKPWYFHLLAKFLDPFMILLEIVAVLCFILFGTNQGSVDNEVNLYIAVVLVFVICLTSVLSFYQEGQSAKVMNTFKNMMSESARVRRGGQEMDVPAEDLVLGDLVVIESGKRVPADLRIIQSDSLKVENSSFTGEPEPVEMCLEKTDDEALHSKNLAFNSAMVVEGKGLGIVVRTGDNTMIGNIASLVGQTKGGKSTLEAEVIGFVHLITKFAILSAIVFYAVALIKTCGFEIGGDCDPAKSFINVFIVILIANVPEGLPATVTSCLTVCAQRLATRQVFVKRLDCVETLGSCTVVASDKTGTLTQNKMTVSHLWLD--CRTRTTEYMT--REGNVDKWDSFKELVTAGALCNMCKYE 435
            ++  K     +  E +  EH  +FE++       +NV  P +S G++   AK  L + G N L PP   PWY  LL  F + FMILLE   VLCFI +  +      ++NLY+  VL  ++  TS+L+F QE QS+ +MN+FKN++ ++ RV R G E  +P E +V+GD+VV+ +G ++PAD+R+I  + +KV+NSS TGE EP    +  TD+  L + NLAF   +V++G   G+V+RTG NT+IG IA L   T   ++TL+ E   FV  I+   I   ++F  V      GF +G    P  + INV  +I +ANVPEGLP+T+T+CLTV A+RL+ R V+ K+L+ +ETLGS T++ASDKTGTLTQN+MTVSH+W D        + MT  R    +K  + + L+  GA CN   ++
Sbjct:  269 SAKDKTKAKQKASEYDVSEHELTFEQIMDKYHTHINVQDPQQSQGISNESAKVLLEKYGRNVLKPPKESPWYLKLLLCFTNFFMILLESAGVLCFIAYALD---TKQKINLYLGCVLWAIVIFTSLLTFAQEWQSSNIMNSFKNLLPQACRVVREGVETKIPVETVVIGDIVVVAAGDKIPADIRVITCNGMKVDNSSLTGESEPQSCTVTCTDENPLETHNLAFYGTLVMDGSARGVVIRTGANTLIGKIADLASNTVQTETTLQKETKRFVRFISGLGISMGVIFLIV------GFIVGIPAIP--NLINVLGLI-VANVPEGLPSTITACLTVTARRLSRRNVYAKKLESIETLGSITLIASDKTGTLTQNRMTVSHMWYDNAIVKAINDGMTVGRAMFNEKDATCETLLRIGACCNRADFD 681