EWM25591.1, cds5084 (CDS) Nannochloropsis gaditana

Overview
NameEWM25591.1
Unique Namecds5084
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002465.1:105663..105777 +
Alignment locationCM002465.1:106047..106207 +
Alignment locationCM002465.1:106416..106538 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM25591.1
Productdna-directed dna polymerase epsilon 2
Orig transcript idgnl|cribi|Naga_101029g1.3279.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002465.1supercontigCM002465.1:105663..105777 +
CM002465.1supercontigCM002465.1:106047..106207 +
CM002465.1supercontigCM002465.1:106416..106538 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Biological Process
TermDefinition
GO:0006259DNA metabolic process
GO:0009058biosynthetic process
Vocabulary: Molecular Function
TermDefinition
GO:0016740transferase activity
GO:0003677DNA binding
Vocabulary: Cellular Component
TermDefinition
GO:0005634nucleus
Vocabulary: INTERPRO
TermDefinition
IPR024639DNA_pol_e_bsu_N
Homology
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|585107383|gb|EWM25591.1| (dna-directed dna polymerase epsilon 2 [Nannochloropsis gaditana])

HSP 1 Score: 268.085 bits (684), Expect = 6.823e-91
Identity = 132/132 (100.00%), Postives = 132/132 (100.00%), Query Frame = 0
Query:    1 MAGRMSSSTRAAANRAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSFTFDKERPFNPDPGAR 132
            MAGRMSSSTRAAANRAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSFTFDKERPFNPDPGAR
Sbjct:    1 MAGRMSSSTRAAANRAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSFTFDKERPFNPDPGAR 132          
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|299470367|emb|CBN78416.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 80.4925 bits (197), Expect = 3.936e-15
Identity = 41/95 (43.16%), Postives = 61/95 (64.21%), Query Frame = 0
Query:   15 RAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYD 109
            +A+FRAFKL+GL ++ADA  A+V V+  EE  D A+ AI+ ++K  +ER EL S +VD  T+  VV  LT  E DL   +  + +AF  P++ +D
Sbjct:    5 QAVFRAFKLQGLGLRADAARAIVRVVEREEGADEAVQAIIRSVKARIERNELSSNVVDVDTVSAVVADLTSREQDLADSSTQMQNAFVRPRLYFD 99          
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|813232892|ref|XP_012213236.1| (hypothetical protein SPRG_18408, partial [Saprolegnia parasitica CBS 223.65] >gi|641519142|gb|KDO16056.1| hypothetical protein SPRG_18408, partial [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 70.8626 bits (172), Expect = 8.479e-12
Identity = 38/122 (31.15%), Postives = 70/122 (57.38%), Query Frame = 0
Query:   11 AAANRAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSFTFDKERPFNPDPGAR 132
            A   + +FRAFKL+GL++ ADA+  L+  +  E  +   LD +L AIK  ++R +L +++V    +E+ +  L     + + ++I ++DAF MP + Y+  +K++S   D+ R  +  P  R
Sbjct:    2 APGAKQVFRAFKLQGLTLHADALKRLLHEMEMEPTL--GLDDVLFAIKNSIDRSKLINSVVTIEALESALDTLLSVSAENDYQSIQVFDAFEMPSLQYEAMTKTYSVGLDRHRRLHASPNYR 121          
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|813171073|ref|XP_012204292.1| (hypothetical protein SPRG_09753 [Saprolegnia parasitica CBS 223.65] >gi|641530924|gb|KDO25023.1| hypothetical protein SPRG_09753 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 70.8626 bits (172), Expect = 9.508e-12
Identity = 38/122 (31.15%), Postives = 70/122 (57.38%), Query Frame = 0
Query:   11 AAANRAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSFTFDKERPFNPDPGAR 132
            A   + +FRAFKL+GL++ ADA+  L+  +  E  +   LD +L AIK  ++R +L +++V    +E+ +  L     + + ++I ++DAF MP + Y+  +K++S   D+ R  +  P  R
Sbjct:    2 APGAKQVFRAFKLQGLTLHADALKRLLHEMEMEPTL--GLDDVLFAIKNSIDRSKLINSVVTIEALESALDTLLSVSAENDYQSIQVFDAFEMPSLQYEAMTKTYSVGLDRHRRLHASPNYR 121          
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|669161178|ref|XP_008616151.1| (hypothetical protein SDRG_11875 [Saprolegnia diclina VS20] >gi|530732056|gb|EQC30298.1| hypothetical protein SDRG_11875 [Saprolegnia diclina VS20])

HSP 1 Score: 70.8626 bits (172), Expect = 1.005e-11
Identity = 40/124 (32.26%), Postives = 72/124 (58.06%), Query Frame = 0
Query:   11 AAANRAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDG--ALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSFTFDKERPFNPDPGAR 132
            A   + +FRAFKL+GL++ ADA+  L+    HE +V+    LD +L AIK  ++R +L +++V    +E+ +  L     + + ++I ++DAF MP + Y+  +K++S   D+ R  +  P  R
Sbjct:    2 APGAKQVFRAFKLQGLTLHADALKRLL----HEMEVEQTLGLDDVLFAIKNSIDRSKLVNSVVTIEALESALDTLLSVSAENDYQSIQVFDAFEMPGLQYEAMTKTYSVGLDRHRRLHAAPNYR 121          
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|397621190|gb|EJK66163.1| (hypothetical protein THAOC_12929 [Thalassiosira oceanica])

HSP 1 Score: 63.1586 bits (152), Expect = 3.401e-10
Identity = 39/108 (36.11%), Postives = 63/108 (58.33%), Query Frame = 0
Query:   11 AAANRAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKG-LVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSF 117
            A   + I R FK RGL++++ A+ AL++VL+ E   D  L AI++ IK  L+  G     +V  + +E VV +L++D  D+  EA+ + DAF  P++ +D   K FS 
Sbjct:    3 ATDRKRIARTFKSRGLTIQSTALDALLNVLSRETH-DETLFAIIDEIKDRLITGGGPGGNVVTTSLLEEVVAELSRDAKDVTDEAVQLLDAFQTPRLEFDATRKQFSL 109          
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|698823517|ref|XP_009844787.1| (hypothetical protein H257_17620 [Aphanomyces astaci] >gi|574100149|gb|ETV65735.1| hypothetical protein H257_17620 [Aphanomyces astaci])

HSP 1 Score: 65.0846 bits (157), Expect = 1.058e-9
Identity = 34/118 (28.81%), Postives = 67/118 (56.78%), Query Frame = 0
Query:   15 RAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSFTFDKERPFNPDPGAR 132
            R +FR  KL GL++ +DA+  L+S LT++  +   LD ++ AIK  ++R +L+S++V +  +E+ +  L     D + + I +++AF  PK+ +   +K++  + D  R  +     R
Sbjct:    5 RQVFRELKLHGLTLHSDALKRLLSELTNQPTLH--LDDVVFAIKNSIDRSKLKSSVVSQDALESALDTLLNVSSDNDYQCIQVFNAFDQPKLIFHPSTKTYEVSADPTRKLHASSAHR 120          
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|1173940795|gb|OQR90110.1| (DNA polymerase epsilon subunit [Achlya hypogyna])

HSP 1 Score: 63.929 bits (154), Expect = 2.249e-9
Identity = 36/121 (29.75%), Postives = 69/121 (57.02%), Query Frame = 0
Query:   12 AANRAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSFTFDKERPFNPDPGAR 132
            AA + +FRAFKL+GL++ ADA+  L+  +  +  V   L+ ++ AIK  ++R +L +++V +  +E+ +  L     + + +AI ++ A   PK+ Y+  SK++    D+ R  +  P  R
Sbjct:    2 AAAKQVFRAFKLQGLTLHADALKRLLREMDEQPSV--LLEDVIYAIKNSIDRSKLTNSVVTQEALESALDTLLSVSTENDYQAIQVFSALEQPKLRYESLSKTYEVVADRGRRLHAAPEYR 120          
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|673043945|ref|XP_008875933.1| (hypothetical protein, variant 3 [Aphanomyces invadans] >gi|574468213|gb|ETV95232.1| hypothetical protein, variant 3 [Aphanomyces invadans])

HSP 1 Score: 63.929 bits (154), Expect = 2.326e-9
Identity = 32/109 (29.36%), Postives = 65/109 (59.63%), Query Frame = 0
Query:   15 RAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSFSFTFDKER 123
            + +FR  KL GL++ +DA+  L+S LT++  +   L+ ++ AIK  ++R +L++++V +  +E+ +  L     D + + I +++AF  PK+ Y   +KS+  + D  R
Sbjct:    5 KQVFRELKLHGLTLHSDALKRLLSELTNQPTL--VLEDVVFAIKNSIDRSKLKTSVVTQDALESALDTLLNVSSDNDYQCIQVFNAFEQPKLVYHPSTKSYEVSADPSR 111          
BLAST of EWM25591.1 vs. NCBI_GenBank
Match: gi|1176068654|ref|XP_020604187.1| (DNA polymerase epsilon subunit 2-like [Orbicella faveolata])

HSP 1 Score: 63.929 bits (154), Expect = 2.711e-9
Identity = 40/112 (35.71%), Postives = 62/112 (55.36%), Query Frame = 0
Query:    4 RMSSSTRAAANRAIFRAFKLRGLSVKADAVAALVSVLTHEEDVDGALDAILNAIKGLVERGELRSTLVDRATIETVVRQLTKDEDDLNQEAIAIWDAFSMPKMTYDHRSKSF 115
            +M+++ R      I   FKL GLS+K DA   L  VL+   +V+  L+  L+ I   V++  L S+LVDR   E  V+  ++  +D N +A  I DAFS+P+ T++   K F
Sbjct:   45 KMAATVRGK----IVSTFKLHGLSLKTDASKFLTEVLSPLTEVE--LEDWLDKIVEGVQKQPLTSSLVDREIAEQAVQDCSQSSEDDNDKAFTIIDAFSVPRFTFNPDRKKF 150          
The following BLAST results are available for this feature:
BLAST of EWM25591.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585107383|gb|EWM25591.1|6.823e-91100.00dna-directed dna polymerase epsilon 2 [Nannochloro... [more]
gi|299470367|emb|CBN78416.1|3.936e-1543.16conserved unknown protein [Ectocarpus siliculosus][more]
gi|813232892|ref|XP_012213236.1|8.479e-1231.15hypothetical protein SPRG_18408, partial [Saproleg... [more]
gi|813171073|ref|XP_012204292.1|9.508e-1231.15hypothetical protein SPRG_09753 [Saprolegnia paras... [more]
gi|669161178|ref|XP_008616151.1|1.005e-1132.26hypothetical protein SDRG_11875 [Saprolegnia dicli... [more]
gi|397621190|gb|EJK66163.1|3.401e-1036.11hypothetical protein THAOC_12929 [Thalassiosira oc... [more]
gi|698823517|ref|XP_009844787.1|1.058e-928.81hypothetical protein H257_17620 [Aphanomyces astac... [more]
gi|1173940795|gb|OQR90110.1|2.249e-929.75DNA polymerase epsilon subunit [Achlya hypogyna][more]
gi|673043945|ref|XP_008875933.1|2.326e-929.36hypothetical protein, variant 3 [Aphanomyces invad... [more]
gi|1176068654|ref|XP_020604187.1|2.711e-935.71DNA polymerase epsilon subunit 2-like [Orbicella f... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna5096rna5096Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications