EWM26474.1, cds4191 (CDS) Nannochloropsis gaditana

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|585108527|gb|EWM26474.1| (manganese transport protein [Nannochloropsis gaditana])

HSP 1 Score: 1149.42 bits (2972), Expect = 0.000e+0
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query:    1 MPPVAAISLPESGLPPSPSISTKLEVSDSMSYTAAENLNQASNSDSSSSTETIYHTFRGKIASFLYMAPPLAASSTAEERLPLRSTTSSQDRNKRTLQVTPQSSSSESSPILTYTTNAPPTRPPTRLLPSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMTVGQGSIPA 576
            MPPVAAISLPESGLPPSPSISTKLEVSDSMSYTAAENLNQASNSDSSSSTETIYHTFRGKIASFLYMAPPLAASSTAEERLPLRSTTSSQDRNKRTLQVTPQSSSSESSPILTYTTNAPPTRPPTRLLPSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMTVGQGSIPA
Sbjct:    1 MPPVAAISLPESGLPPSPSISTKLEVSDSMSYTAAENLNQASNSDSSSSTETIYHTFRGKIASFLYMAPPLAASSTAEERLPLRSTTSSQDRNKRTLQVTPQSSSSESSPILTYTTNAPPTRPPTRLLPSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMTVGQGSIPA 576          

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|553188746|ref|XP_005854858.1| (mn2+ fe2+ nramp family [Nannochloropsis gaditana CCMP526] >gi|422294201|gb|EKU21501.1| mn2+ fe2+ nramp family [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 516.153 bits (1328), Expect = 9.845e-178
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0

Query:    1 MPPVAAISLPESGLPPSPSISTKLEVSDSMSYTAAENLNQASNSDSSSSTETIYHTFRGKIASFLYMAPPLAASSTAEERLPLRSTTSSQDRNKRTLQVTPQSSSSESSPILTYTTNAPPTRPPTRLLPSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIG 254
            MPPVAAISLPESGLPPSPSISTKLEVSDSMSYTAAENLNQASNSDSSSSTETIYHTFRGKIASFLYMAPPLAASSTAEERLPLRSTTSSQDRNKRTLQVTPQSSSSESSPILTYTTNAPPTRPPTRLLPSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIG
Sbjct:    1 MPPVAAISLPESGLPPSPSISTKLEVSDSMSYTAAENLNQASNSDSSSSTETIYHTFRGKIASFLYMAPPLAASSTAEERLPLRSTTSSQDRNKRTLQVTPQSSSSESSPILTYTTNAPPTRPPTRLLPSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIG 254          

HSP 2 Score: 213.001 bits (541), Expect = 6.807e-60
Identity = 118/134 (88.06%), Postives = 123/134 (91.79%), Query Frame = 0

Query:  306 IFVCFAAEIVLSRPSFS---GVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLG 436
            +F+   AEI ++    +   GVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLG
Sbjct:  234 VFLWITAEIAIAACDLAEVIGVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLG 367          

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|1199705219|ref|WP_087435729.1| (divalent metal cation transporter [Paenibacillus apiarius])

HSP 1 Score: 498.049 bits (1281), Expect = 1.297e-169
Identity = 270/440 (61.36%), Postives = 331/440 (75.23%), Query Frame = 0

Query:  129 PSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMT 568
            PSLPEAY S+PVP+         S  R  LA+VGPGY+VAVGYMDPGNWAT IAGG+ ++Y LLSVVL+S+L+A+ LQ L+ +LG+AT RDLAQ C + YS      LWI  E+AIAACDLAE+IGSAIALQLLFGLPL+ G  +T+LD+LIVL   +K    FR +E+ V+ LIA I +CF AE++LSRP    V+ G FVP    +   A  LYIA+GILGATVMPHNLYLHSS+VQTR+Y ES E K  AI +++LD+ IAL LA+FVNAAILI++AA  F  SG T+ A  + +AY +L P+LG   AS+LF VALLASGQNSTLTGTLAGQIVMEGF+Q+RLKPW+RRLITRL+ IVPAV  + + G++G   LL+LSQVILS QLSFAVFPL+ FTSD   MG  FV+    + LAWVS I+IA LN++LLV T
Sbjct:   24 PSLPEAYKSMPVPKKG-------SFFRKFLAFVGPGYLVAVGYMDPGNWATAIAGGSQFNYTLLSVVLISNLMAMLLQSLSARLGIATGRDLAQACHDHYSKPVNFVLWILCELAIAACDLAEIIGSAIALQLLFGLPLIIGVIITSLDILIVLLLQNKG---FRYIEVIVVVLIATIMMCFGAELILSRPDVREVLHG-FVPSAEIVKNPA-MLYIALGILGATVMPHNLYLHSSIVQTRNYEESYEGKKSAIRFATLDSTIALILAMFVNAAILILSAAT-FHSSGHTEIA-EIQDAYHMLTPLLGTGMASVLFAVALLASGQNSTLTGTLAGQIVMEGFLQIRLKPWVRRLITRLIAIVPAVIVIAIYGEQGTADLLILSQVILSLQLSFAVFPLIRFTSDTKKMGA-FVSPLWMNSLAWVSGIMIAGLNLYLLVGT 448          

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|754586341|ref|WP_041978172.1| (divalent metal cation transporter [Pyrinomonas methylaliphatogenes] >gi|746990502|emb|CDM66746.1| natural resistance-associated macrophage protein metal ion transporter NRAMP [Pyrinomonas methylaliphatogenes])

HSP 1 Score: 496.508 bits (1277), Expect = 5.179e-169
Identity = 270/439 (61.50%), Postives = 327/439 (74.49%), Query Frame = 0

Query:  130 SLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMT 568
            SLPEAY ++ VP+ R G   W    R +LA+ GPGYMVAVGYMDPGNWATDIA G+ ++Y LLSV++LS+L+A+ LQ L V+LGVAT RDLAQ C + YSPR +  LW+  EIAI ACDLAEVIGSAIAL LLFG+PLVWG  LTALDV+++L       R FR LE  V+ LI VI VCFAAEI+ SRP  SG+++G F+P    + T +  LYIA+GILGATVMPHNLYLHSS+VQTR Y  S   K EAI ++++D+ IAL LALF+N AIL+VAAA  F  +G+   A  + +AY LL+P+LG   AS LF +ALLASGQNSTLTGTLAGQIVMEGF+ +RL+PWLRRLITRLV IVPA+    L G+ G   LL+ SQV+LS QLSFAVFPLV FTSD   MG EFVN     +LAW  A++IA+ NIWLL+ T
Sbjct:   22 SLPEAYRALGVPQ-RAG--FW----RKMLAFSGPGYMVAVGYMDPGNWATDIAAGSRFNYTLLSVIMLSNLMAILLQSLAVRLGVATGRDLAQACRDHYSPRVSFILWVLCEIAIVACDLAEVIGSAIALNLLFGIPLVWGVMLTALDVILLLML---QNRGFRLLEAIVISLIIVIGVCFAAEIIFSRPDVSGILRG-FIPS-PQIITDSGMLYIAIGILGATVMPHNLYLHSSVVQTRRYELSAAGKREAIRFATIDSTIALMLALFINGAILVVAAAT-FHRTGNYAVA-EIQDAYKLLSPLLGVSVASTLFAIALLASGQNSTLTGTLAGQIVMEGFLNIRLRPWLRRLITRLVAIVPAIIVTALYGESGTAQLLIFSQVVLSMQLSFAVFPLVKFTSDPRKMG-EFVNPAWLKWLAWTVAVIIASFNIWLLLQT 445          

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|518252781|ref|WP_019422989.1| (divalent metal cation transporter [Paenibacillus sp. OSY-SE])

HSP 1 Score: 494.582 bits (1272), Expect = 2.675e-168
Identity = 261/441 (59.18%), Postives = 332/441 (75.28%), Query Frame = 0

Query:  129 PSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMTV 569
            PSLPEAY S+PVP+         S  R  LA+VGPGY++AVGYMDPGNWATDIAGGA ++Y LLSV+L+S+L+A+ LQ L+ +LG+AT RDLAQ C + YS      LWI  E+AIAACDLAE+IGSAIALQLLFGLPL+ G  +T+LD+LIV+   +K    FR +E+ ++ LIA I +CF  E++LSRP    V+ G F+P    +   A  LYIA+GILGATVMPHNLYLHSS+VQTR+Y ES E K  AI +++LD+ IAL +A+FVNAAILI++AA F+  SG T+ A  + +AY +L P+LG   AS+LF VALLASGQ+STLTGTLAGQIVMEGF+Q+RLKPW+RRLITRL+ IVP V  + + G++G   LL+LSQVILS QLSFAVFPL+ FTSD   MG  FV+    + LAW++ I+IA LN++LLV T+
Sbjct:   24 PSLPEAYKSMPVPKKG-------SFFRKFLAFVGPGYLIAVGYMDPGNWATDIAGGAQFNYTLLSVILISNLMAMLLQSLSARLGIATGRDLAQACHDHYSKPVNFVLWILCELAIAACDLAEIIGSAIALQLLFGLPLIIGVIITSLDILIVMLLQNKG---FRYVEVIIVVLIATIMMCFGTELILSRPDVQEVLHG-FIPSAEIVKNPA-MLYIALGILGATVMPHNLYLHSSIVQTRNYEESYEGKKSAIRFATLDSTIALMMAMFVNAAILILSAATFYS-SGHTEIA-EIQDAYQMLTPLLGTGMASVLFAVALLASGQSSTLTGTLAGQIVMEGFLQIRLKPWVRRLITRLIAIVPTVIVIAIYGEQGTAELLILSQVILSLQLSFAVFPLIRFTSDKKKMGA-FVSPLWMNSLAWIAGIIIAGLNLYLLVRTL 449          

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|497316610|ref|WP_009630823.1| (divalent metal cation transporter [Synechocystis sp. PCC 7509])

HSP 1 Score: 491.115 bits (1263), Expect = 4.365e-167
Identity = 266/447 (59.51%), Postives = 331/447 (74.05%), Query Frame = 0

Query:  123 PPTRLLPSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPR-LYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMT 568
            P  R  PSLPE + S+ +P    G+  W    R +LAY GPGY+V+VGYMDPGNWATDIAGG+ + Y LLSV+LLS+L+A+ LQ L V+LGVAT RDLAQ C + +SPR    LW+  EIAIAACDLAE++GSAIALQLLFG+PL+WG C+TALDVL++L F    G  FR +E  V+ L+A + +CF AEI+ S+P   G++ G + P +  L  Q P  LYIA+GILGATVMPHNLYLHSS+VQTRD+  + E K EAI + ++D+ +ALSLALF+N+AILIVAAA  F  SG+ QD   + +AY LL+P+LG  AAS +FG+ALLASGQ+STLT TLAGQ+VMEGF+ +RL  WLRRL TR + IVPAV T++L G++    LLV SQVILS QLSFAVFPLVMFTS   +MG EFVN      LAW  A +IA LNIWLL+ T
Sbjct:    4 PQNR--PSLPEVHRSIKIPE---GKGFW----RKMLAYAGPGYLVSVGYMDPGNWATDIAGGSKFGYTLLSVILLSNLMAILLQSLCVRLGVATGRDLAQACRDYFSPRINFCLWVLCEIAIAACDLAELLGSAIALQLLFGIPLLWGVCITALDVLVLL-FLQNKG--FRYVEALVITLVATVGICFTAEIIFSQPDAGGILLG-YAPKIEIL--QNPEMLYIAIGILGATVMPHNLYLHSSIVQTRDFQPTSEKKWEAIKFGTIDSTVALSLALFINSAILIVAAAT-FHFSGN-QDVAEIQDAYKLLSPLLGVGAASAIFGLALLASGQSSTLTATLAGQVVMEGFLHLRLPSWLRRLATRALAIVPAVITIILFGEQSTGKLLVFSQVILSLQLSFAVFPLVMFTSKRRLMG-EFVNPLWLKVLAWTVAFVIAGLNIWLLIQT 432          

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|1210107840|dbj|BAY26970.1| (manganese transport protein [Calothrix sp. NIES-2100])

HSP 1 Score: 491.115 bits (1263), Expect = 5.194e-167
Identity = 252/441 (57.14%), Postives = 331/441 (75.06%), Query Frame = 0

Query:  129 PSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPRLYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMTV 569
            PSLPE + S+ +P+ +       +  R +LAY GPGY+V+VGYMDPGNWATD+AGGA + YALL+V++LS+L+A+ +Q L V+LGVAT+RDLAQ C + +SPR   +LW+  EIAIAACDLAEV+GSAIALQLLFG+PL WG C+TALDV+++L    K    FR  E  V+ L+A + +CF AEI+ SRP F G++ G +VP +  +      LYIA+GILGATVMPHNLYLHSS+VQTR++  + + K EAI + ++D+ +AL  ALF+N+AILIVAAA  F  SG  QD   + +AY LL+P+LG  AAS +F +ALLASGQ+STLT TLAGQIVMEGF+ +RLKPWLRRLITRL+ I+PA+ +++  G+K    LL+ SQVILS QLSFAV PLVMFTS+  +MG EFVN      L+W+ AI+I +LN+WLL+ T+
Sbjct:   11 PSLPEVHRSIEIPKTK-------NIWRKMLAYAGPGYLVSVGYMDPGNWATDLAGGAKFGYALLTVIMLSNLMAILMQSLCVRLGVATERDLAQACRDYFSPRVNFWLWVLCEIAIAACDLAEVLGSAIALQLLFGIPLSWGVCITALDVIVLLLLQHKG---FRYTEALVIMLVATVGLCFFAEIIFSRPDFGGILLG-YVPKI-EIVQNPEMLYIAIGILGATVMPHNLYLHSSIVQTRNWEATSQKKWEAIKFGTIDSTVALFFALFINSAILIVAAAT-FHFSG-YQDVAEIQDAYKLLSPILGVNAASAIFALALLASGQSSTLTATLAGQIVMEGFLNLRLKPWLRRLITRLIAIIPALISIIFLGEKSTGSLLIFSQVILSLQLSFAVVPLVMFTSNRRLMG-EFVNPLWLKILSWLVAIVIGSLNVWLLLQTI 436          

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|1209852735|dbj|GAX43548.1| (manganese transport protein [Tolypothrix sp. NIES-4075])

HSP 1 Score: 491.115 bits (1263), Expect = 6.221e-167
Identity = 255/442 (57.69%), Postives = 329/442 (74.43%), Query Frame = 0

Query:  129 PSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPR-LYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMTV 569
            PSLPE + S+ +P          +  R +LAY GPGY+V+VGYMDPGNWATD+AGGA + YALL+V++LS+L+A+ +Q L V+LGVAT RDLAQ C E +SPR    LW+  EIAIAACDLAEV+GSAIALQLLFG+PL WG C+TALDV+++L    K    FR  E  V+ L+A + +CF AEI+ SRP F G++ G + P +  +  Q P  LYIA+GILGATVMPHNLYLHSS+VQTRD+  + E K EAI + ++D+ +ALS ALF+N+AILI++AA+ F  SG   D   + +AY LL+P+LG  AAS +FG+ALLASGQ+STLT TLAGQIVMEGF+ +RLKPWLRRL TRL+ IVPA+ +++  G+K    LL+LSQV+LS QLSFAV PLVMFTS+  +MG EFVN      L+W+ AI I +LN+WLL+ T+
Sbjct:   11 PSLPEVHRSIAIPNTT-------NVWRKMLAYAGPGYLVSVGYMDPGNWATDLAGGAKFGYALLTVIMLSNLMAILMQSLCVRLGVATGRDLAQACREYFSPRVNFCLWLLCEIAIAACDLAEVLGSAIALQLLFGIPLSWGVCITALDVIVLLLLQHKG---FRYTEALVIMLVATVGICFTAEIIFSRPDFGGILLG-YAPKIEIV--QNPEMLYIAIGILGATVMPHNLYLHSSIVQTRDWQPTTEKKWEAIKFCTIDSTVALSFALFINSAILIISAAS-FHFSGH-HDVAEIQDAYKLLSPLLGVNAASAIFGLALLASGQSSTLTATLAGQIVMEGFLNLRLKPWLRRLTTRLIAIVPALISIMFLGEKSTGGLLILSQVVLSLQLSFAVVPLVMFTSNHRLMG-EFVNPLWLKILSWLVAIAIGSLNVWLLLQTI 436          

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|1197831900|ref|WP_086766539.1| (MULTISPECIES: divalent metal cation transporter [Nostoc] >gi|1195998027|gb|OUL20416.1| divalent metal cation transporter [Nostoc sp. RF31Y] >gi|1195999726|gb|OUL22057.1| divalent metal cation transporter [Nostoc sp. 106C])

HSP 1 Score: 490.73 bits (1262), Expect = 8.187e-167
Identity = 258/452 (57.08%), Postives = 335/452 (74.12%), Query Frame = 0

Query:  119 PPTRPPTRLLPSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPR-LYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMTV 569
            P +  P R  PSLPE + S+ +P+ +       +  R +LAY GPGY+V+VGYMDPGNWATD+AGGA + YALL+V++LS+L+A+ +Q L V+LGVAT RDLAQ C + +SPR    LW+  EIAIAACDLAEV+GSAIALQLLFG+PL WG C+TALDV+++L    K    FR  E  V+ L+A + +CF AEI+ SRP F G++ G +VP +  L  Q P  LYIA+GILGATVMPHNLYLHSS+VQTR++  + E K EAI +S++D+ +AL  ALF+N+AILIV+AA  F  SG   D   + +AY LL+P+LG  AAS +F +ALLASGQ+STLT TLAGQIVMEGF+ +RLKPWLRRLITRL+ I+PA+ +++  G+K    LL+ SQVILS QLSFAV PLVMFTS+  +MG EFVN      L+W+ AI+I +LN+WLL+ T+
Sbjct:    2 PFSENPDRK-PSLPEVHRSIAIPKTK-------NIWRKMLAYAGPGYLVSVGYMDPGNWATDLAGGAKFGYALLTVIMLSNLMAILMQSLCVRLGVATGRDLAQACRDYFSPRVNFCLWVLCEIAIAACDLAEVLGSAIALQLLFGIPLSWGVCITALDVIVLLLLQHKG---FRYTEALVIMLVATVGLCFLAEIIFSRPDFGGILLG-YVPKIEIL--QNPEMLYIAIGILGATVMPHNLYLHSSIVQTRNWEVTSEKKWEAISFSTIDSTVALFFALFINSAILIVSAAT-FHFSG-YHDVAEIKDAYKLLSPILGVNAASAIFALALLASGQSSTLTATLAGQIVMEGFLNLRLKPWLRRLITRLIAIIPALISIIFFGEKSTSGLLIFSQVILSLQLSFAVVPLVMFTSNRRLMG-EFVNPLWLKILSWLVAIVIGSLNVWLLLQTI 436          

BLAST of EWM26474.1 vs. NCBI_GenBank
Match: gi|1210212649|dbj|BAZ31689.1| (manganese transport protein [Cylindrospermum sp. NIES-4074])

HSP 1 Score: 488.804 bits (1257), Expect = 4.171e-166
Identity = 256/458 (55.90%), Postives = 336/458 (73.36%), Query Frame = 0

Query:  120 PTRPPTRLLPSLPEAYHSVPVPRARPGEVSWLSRCRTLLAYVGPGYMVAVGYMDPGNWATDIAGGAIYDYALLSVVLLSSLLAVFLQVLTVKLGVATKRDLAQMCAEAYSPRFAVFLWITAEIAIAACDLAEVIGSAIALQLLFGLPLVWGCCLTALDVLIVLSFFDKPGRRFRGLELAVMGLIAVIFVCFAAEIVLSRPSFSGVMKGFFVPDLYALATQAPR-LYIAMGILGATVMPHNLYLHSSLVQTRDYGESVEDKTEAIFYSSLDTIIALSLALFVNAAILIVAAAAFFDPSGSTQDAPSVSEAYDLLAPVLGNKAASILFGVALLASGQNSTLTGTLAGQIVMEGFVQVRLKPWLRRLITRLVVIVPAVFTVVLAGDKGVDFLLVLSQVILSFQLSFAVFPLVMFTSDVSVMGTEFVNSPSTSFLAWVSAILIAALNIWLLVMTVGQGSIPA 576
            P  P  +  PSLPE + S+ +P  +       +  R +LAY GPGY+V+VGYMDPGNWATD+AGG+ + YALL+V++LS+L+A+ +Q L V+LGVAT RDLAQ C + +SPR   +LW+  EIAIAACDLAEV+GSAIALQLLFG+PL WG C+TALDV+++L    K    FR  E  V+ L+A + +CFAAEI+ S P   G++ G F P +  +  Q P  LYIA+GILGATVMPHNLYLHSS+VQTRD+  + E + EAI + ++D+ +AL  ALF+N+AILIVAAA  F  SG+ QD   + +AY LL+P+LG  AAS +FG+ALLASGQ+STLT TLAGQIVMEGF+ +RLKPWLRRL+TRL+ I+PA+ +++  G+K    LL+ SQV+LS QLSFAV PLVMFTS+  +MG EFVN      L+W+ AI+I +LNIWLL+ T+     P+
Sbjct:    4 PEDPARK--PSLPEVHRSIAIPNTK-------NIWRKMLAYAGPGYLVSVGYMDPGNWATDLAGGSKFGYALLTVIMLSNLMAILIQSLCVRLGVATGRDLAQACRDYFSPRVNFWLWVLCEIAIAACDLAEVLGSAIALQLLFGIPLGWGVCITALDVIVLLLLQHK---GFRYTEALVIMLVATVGICFAAEIIFSHPDLGGIILG-FAPKIEII--QNPEMLYIAIGILGATVMPHNLYLHSSIVQTRDWEPTSEKRREAIKFGTIDSTVALFFALFINSAILIVAAAT-FHFSGN-QDVAEIQDAYKLLSPLLGVNAASAIFGLALLASGQSSTLTATLAGQIVMEGFLNLRLKPWLRRLVTRLIAIIPALISIMFFGEKSTSSLLIFSQVVLSLQLSFAVVPLVMFTSNHRLMG-EFVNPLWLKILSWLVAIVIGSLNIWLLLQTISGWMFPS 443