EWM27529.1, cds3114 (CDS) Nannochloropsis gaditana

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|585109796|gb|EWM27529.1| (nadh-dependent fumarate reductase [Nannochloropsis gaditana])

HSP 1 Score: 2706.01 bits (7013), Expect = 0.000e+0
Identity = 1315/1315 (100.00%), Postives = 1315/1315 (100.00%), Query Frame = 0

Query:    1 MAVLSVSLDAGRVDSIFATYLWRNEMREGIHRHFNTLLNLRPAPQLFNHPMTLFINPSKILHAMASLADGRAAASAVVAPMNLRKPAAGPFTASAAGGWNDPALNMEEGELKKVLQTVTGTRGAMQYIAKVGAPPERLDEIHALVEDVLKRAEQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVGDKLIHHVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQENGGSTTHVPAQKTVTGVTYEDMATHEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAIGVCLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLEFTGSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEMVMCKMLGEIGVPQHAVFAYSNP 1315
            MAVLSVSLDAGRVDSIFATYLWRNEMREGIHRHFNTLLNLRPAPQLFNHPMTLFINPSKILHAMASLADGRAAASAVVAPMNLRKPAAGPFTASAAGGWNDPALNMEEGELKKVLQTVTGTRGAMQYIAKVGAPPERLDEIHALVEDVLKRAEQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVGDKLIHHVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQENGGSTTHVPAQKTVTGVTYEDMATHEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAIGVCLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLEFTGSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEMVMCKMLGEIGVPQHAVFAYSNP
Sbjct:    1 MAVLSVSLDAGRVDSIFATYLWRNEMREGIHRHFNTLLNLRPAPQLFNHPMTLFINPSKILHAMASLADGRAAASAVVAPMNLRKPAAGPFTASAAGGWNDPALNMEEGELKKVLQTVTGTRGAMQYIAKVGAPPERLDEIHALVEDVLKRAEQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVGDKLIHHVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQENGGSTTHVPAQKTVTGVTYEDMATHEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAIGVCLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLEFTGSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEMVMCKMLGEIGVPQHAVFAYSNP 1315          

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|1129176950|gb|OLP85325.1| (putative fumarate reductase [Symbiodinium microadriaticum])

HSP 1 Score: 914.45 bits (2362), Expect = 0.000e+0
Identity = 518/1176 (44.05%), Postives = 716/1176 (60.88%), Query Frame = 0

Query:  140 EIHALVEDVLKRAEQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFW--VGDKLIHHVFQSKT-KRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVP-KEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEP-ARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQENGGSTTHVPAQKTVTGVTYEDMATHEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPY-VGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAIGVCLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVL---EGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLE---FTGSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEMVMCKMLGEIG 1303
            E   +V++V    E+ FS +++DSEV+++N+  +      S  M  VL A   +  +T+G +DPA  PL  HYK+             VE  P +  I+ ++ W +F ++++G  +TK HD A +DL GLAKGWAID+    L+  GF   +++WGGD+ V+G HPTGR W+  V+EP  ++                                    S   +   E +EAE +                   A+I LRDG ++ TSGDY+Q        K   HV   ++ K+L+++S   +A AS+V   SCM+ADALAT+ALA+G    +E+R  L+  +       VTD+LL+SR GP+V+R R  GSE    R++R + HE  A VV+ GGGLAG SAA+EA K  A V LLEKEA++GGNSAKATSGIN  GT  Q   GV D+ ++ ERDT  S KGG  D   + +L+++SAEA+ WL+D++G+ L ELSQLGGHA KRTHR  P   G P P+G+ IM+  +A     +    + ++T++ + +L +Q+ +E+G           V GV Y D A  E+ TL  DAV+LTTGG+G D S  SL+K++RPDL            +G+P+TNG+FA GDG++ G  +GA  VDMDKVQLHPT+ +DPKDP+  TK LGPEALRGSGGIL++Q+GRRFVNELDLRS VS  IL  C+ Y +    + G  ++WCVLN+E QQ FGL  L FYK ++GLFE       LA  ++GCE   +  T ++YA +    +C KT K  FP  +   D   V AR+TP IHY MGGL IS + EVL   +GA   R  ++       RLFAAGE TGGVHG NRLGGNSLLECVVFGR+AG +A  I Q+    L    W PV  RE+  TD +YG NT V+RF LHG++Q  GL VG+++ I+GE+DG+ + GFYSP+SRP++ G ++ILCRTD  GG I++ LN+++PG  C +  MGG++L+    TG   +QG R IK+LSLL GGTG+AP +Q+VRAY+  +  +  PE  P+  P   GV++VYAAE   DLAF   L  ++  +P    +Y+VLNKPP GW +G+GFV+ D  R+ L++P  E    + V+CGPP+FE +MC  L ++G
Sbjct:  150 EAQRIVDEVFGETEKTFSHFVEDSEVSQLNRLAKKTLFKPSPAMKKVLSAAGTINRMTRGAFDPAILPLAAHYKK----------TPGVE--PSSDLIS-HSKWSAFEITEEG--VTKQHDSAMMDLCGLAKGWAIDEMSQRLKDFGFTASYVDWGGDIKVSGQHPTGRNWTAAVLEPYPLNG-----------------------------------SNEPEKADEGKEAEHI-------------------AHIELRDGQAIATSGDYLQQLADDKNQKTRCHVLDGRSDKKLVQISHHSVASASVVCS-SCMIADALATSALAVGETSVRESRKLLEVFTGHALKDPVTDFLLFSRLGPRVVRWRPPGSEAQEHRELRHASHEEGAHVVIVGGGLAGVSAAVEAVKAKATVTLLEKEAQLGGNSAKATSGINACGTRVQKAYGVDDDVRWLERDTFVSAKGGSSDQGCVSMLASKSAEAIHWLIDELGIPLSELSQLGGHAKKRTHRCPPKADGTPVPVGYTIMQHARAAA---MAIPGVSVKTNSTMKRL-LQKTREDGA--------LEVCGVEYMD-ADGELQTLSCDAVILTTGGFGFDHSDGSLMKQYRPDL------------VGVPTTNGSFANGDGMRFGADVGASLVDMDKVQLHPTALIDPKDPLCHTKYLGPEALRGSGGILLDQQGRRFVNELDLRSTVSAKILEHCEEYHIEGSAEKGRPWSWCVLNQECQQKFGLPMLGFYK-SQGLFEEVTGTAGLAA-LLGCEEQTILETFKDYANACDQKICQKTGKTVFPSKVTEEDQSFVVARITPCIHYCMGGLEISPSGEVLTRKDGAMGKRSKIH-------RLFAAGECTGGVHGGNRLGGNSLLECVVFGRIAGEKAAAINQKEQGLLSSGTWLPVKLREIRDTDERYGHNTAVYRFELHGSMQTTGLEVGRYIGIKGEIDGDTVTGFYSPISRPEDSGVIDILCRTDEKGGPIVSLLNSMQPGSSCMMAGMGGVKLKQDVTTGGFSYQG-RSIKKLSLLCGGTGLAPAVQVVRAYVNMI--SKFPEQMPI-SPDEGGVKIVYAAETHGDLAFITALDQLKERFPQLISYYLVLNKPPRGWVQGIGFVDGDIIRQRLWFPGSED--HVCVMCGPPIFEKIMCANLAKMG 1215          

HSP 2 Score: 912.909 bits (2358), Expect = 0.000e+0
Identity = 521/1193 (43.67%), Postives = 720/1193 (60.35%), Query Frame = 0

Query:  140 EIHALVEDVLKRAEQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFW--VGDKLIHHVFQSKT-KRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVP-KEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEP-ARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQENGGSTTHVPAQKTVTGVTYEDMATHEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPY-VGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAIGVCLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVL---EGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLE---FTGSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEMVMCKMLGEIGV----------PQHAVF 1310
            E   +V++V    E+ FS +++DSEV+++N+  +      S  M  VL A   +  +T+G +DPA  PL  HYK+             VE  P +  I+ ++ W +F ++++G  +TK HD A +DL GLAKGWAID+    L+  GF   +++WGGD+ V+G HPTGR W+  V+EP  ++                                    S   +   E +EAE +                   A+I LRDG ++ TSGDY+Q        K   HV   ++ K+L+ +S   +A AS+V   SCM+ADALAT+ALA+G    +E+R  L+  +       VTD+LL+SR GP+V+R R  GSE    R++R + HE  A VV+ GGGLAG SAA+EA K  A V LLEKEA++GGNSAKATSGIN  GT  Q   GV D+ ++ ERDT  S KGG  D   + +L+++SAEA+ WL+D++G+ L ELSQLGGHA KRTHR  P   G P P+G+ IM+  +A     +    + ++T++ + +L +Q+ +E+G           V GV Y D A  E+ TL  DAV+LTTGG+G D S  SL+K++RPDL            +G+P+TNG+FA GDG++ G  +GA  VDMDKVQLHPT+ +DPKDP+  TK LGPEALRGSGGIL++Q+GRRFVNELDLRS VS  IL  C+ Y +    + G  ++WCVLN+E QQ FGL  L FYK ++GLFE       LA  ++GCE   +  T ++YA +    +C KT K  FP  +   D   V AR+TP IHY MGGL IS + EVL   +GA   R  ++       RLFAAGE TGGVHG NRLGGNSLLECVVFGR+AG +A  I Q+    L    W PV  RE+  TD +YG NT V+RF LHG++Q  GL VG+++ I+GE+DG+ + GFYSP+SRP++ G ++ILCRTD  GG I++ LN+++PG  C +  MGG++L+    TG   +QG R IK+LSLL GGTG+AP +Q+VRAY+  +  +  PE  P+  P   GV++VYAAE   DLAF   L  ++  +P    +Y+VLNKPP GW +G+GFV+ D  R+ L++P  E    + V+CGPP+FE +MC  L ++G           P+H+ F
Sbjct: 1392 EAQRIVDEVFGETEKTFSHFVEDSEVSQLNRLAKKTLFKPSPAMKKVLSAAGTINRMTRGAFDPAILPLAAHYKK----------TPGVE--PSSDLIS-HSKWSAFEITEEG--VTKQHDSAMMDLCGLAKGWAIDEMSQRLKDFGFTASYVDWGGDIKVSGQHPTGRNWTAAVLEPYPLNG-----------------------------------SNEPEKADEGKEAEHI-------------------AHIELRDGQAIATSGDYLQQLADDKNQKTRCHVLDGRSDKKLVLISHHSVASASVVCS-SCMIADALATSALAVGETSVRESRKLLEVFTGHALKDPVTDFLLFSRLGPRVVRWRPPGSEAQEHRELRHASHEEGAHVVIVGGGLAGVSAAVEAVKAKATVTLLEKEAQLGGNSAKATSGINACGTRVQKAYGVDDDVRWLERDTFVSAKGGSSDQGCVSMLASKSAEAIHWLIDELGIPLSELSQLGGHAKKRTHRCPPKADGTPVPVGYTIMQHARAAA---MAIPGVSVKTNSTMKRL-LQKTREDGA--------LEVCGVEYMD-ADGELQTLSCDAVILTTGGFGFDHSDGSLMKQYRPDL------------VGVPTTNGSFANGDGMRFGADVGASLVDMDKVQLHPTALIDPKDPLCHTKYLGPEALRGSGGILLDQQGRRFVNELDLRSTVSAKILEHCEEYHIEGSAEKGRPWSWCVLNQECQQKFGLPMLGFYK-SQGLFEEVTGTAGLAA-LLGCEEQTILETFKDYANACDQKICQKTGKTVFPSKVTEEDQSFVVARITPCIHYCMGGLEISPSGEVLTRKDGAMGKRSKIH-------RLFAAGECTGGVHGGNRLGGNSLLECVVFGRIAGEKAAAINQKEQGLLSSGTWLPVKLREIRDTDERYGHNTAVYRFELHGSMQTTGLEVGRYIGIKGEIDGDTVTGFYSPISRPEDSGVIDILCRTDEKGGPIVSLLNSMQPGSSCMMAGMGGVKLKQDVTTGGFSYQG-RSIKKLSLLCGGTGLAPAVQVVRAYVNMI--SKFPEQMPI-SPDEGGVKIVYAAETHGDLAFITALDQLKERFPQLISYYLVLNKPPRGWVQGIGFVDGDIIRQRLWFPGSED--HVCVMCGPPIFEKIMCANLAKMGYDRGYENWPLKPRHSSF 2474          

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|342184056|emb|CCC93537.1| (unnamed protein product [Trypanosoma congolense IL3000])

HSP 1 Score: 777.704 bits (2007), Expect = 0.000e+0
Identity = 491/1185 (41.43%), Postives = 676/1185 (57.05%), Query Frame = 0

Query:  128 IAKVGAPPERLDEIHALVEDVLKRAEQVFSLWIK----DSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVG-DKLIHHVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQENGGSTTHVPAQKTVTGVTYE--DMATHEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQ-SAAIGVCLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLEFTGSQ---IFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEM-VMCKMLG 1300
            +  V   P+   E+    E +L+ A Q+ +  +     +SEV+++N  P G    +SE +  V+     +Y+ T   +DPA +P++   +Q ++      D+T  E++ +  ++       SF L+ +  T+T+ H++A LDLGGL KG+ +D  V  LR AG  ++  EWGGDV V+G +  G  W V +  PPSV  +                                        ++  +E E    D         EE+ P L  + L D  ++ TSGDY  + +     ++ ++F  + +RL+      LAQ S+    S M ADALATA L +   P   R  L+  + R     VT+Y  Y+R+G ++        E    R+ R++   P+RVV+ GGGLAG SAAIEAA  GA+V+L+EKE ++GGNSAKATSGINGWGT  QA   VMD  KYFERDT  SG GG  DP+L+++LS +S +A+ WL   +GV L  LSQLGGH++KRTHRA     G P PIG+ IM+ ++ ++R+ L  + + I T   V  L M E     G  T V     VTGVTY   D     +  L AD+V+L TGG+  D    SLL+E+ P L               P+TNG +ATGDGVKL  ALGA  VDMDKVQLHPT  +DPK+P N TKILGPEALRGSGGIL+N++GRRFVNELDLRSVVS AI  Q   Y  ++   G  +A+CVLN+EA + FG++AL FY    GLF+  +    LA  +IGC+ A L+ T++ Y + S+    C  T K  FP  +       VA  VTPSIHYTMGG  IS AAE+L   +    N+    RP+R LF AGEVTGGVHG NRLGGNSLLECVVFG++AG RA TI QRR   L +  WT V  RE  ++  ++GV + V RF+L GALQ+ GL++G+FVAIRGE DG+ L G+YSP++ PD+ G + +L R D   G +  +++A+RPG    +KA GGLR++    +   +F+G   I+ L L++ GTGIAPM+Q++RA L          ++P  D   S +RL+YAAE    L +   L+   + YP  F    VLN PP GWT GVGFV+  + ++ L  P   S+  L  ICGPP  +  V  ++LG
Sbjct:  115 LCVVTCNPKAATELEPKAEAILREAFQMVNNHLNSFNPNSEVSQLNALPVGVKHQMSEHLHKVMECALHVYNSTGADFDPATAPIVSRLRQAVRNPDIVSDLTLSEKEVEHFSLPR-----SFELNLEEGTITRKHEEARLDLGGLNKGYTVDYVVNNLRAAGMENVLFEWGGDVRVSGRNIKGHQWVVALARPPSVEEV----------------------------------------LYRARENEVSTHD--------NEESTPLLHVVEL-DNEALCTSGDYENIIYHPVHGVVGNIFDWRWRRLLSPEEGALAQVSVKCY-SAMYADALATACL-IKRDPVRVRYLLE--NWRYLRNRVTNYFAYTRQGERLAHMHEIAQETRELREERIAGSIPSRVVIVGGGLAGLSAAIEAASCGAQVILMEKEQKLGGNSAKATSGINGWGTRAQAESDVMDGGKYFERDTFLSGSGGSTDPALVKMLSVKSGDAIGWLT-SLGVPLTVLSQLGGHSYKRTHRAPDKADGTPVPIGYTIMKILEDHIRKNL-RNNVTIMTEVAVKGL-MHETSTTPGGATEV----RVTGVTYTTADDGFCRLLELRADSVILATGGFSNDREPNSLLQEYAPQL------------SSFPTTNGPWATGDGVKLAQALGAKLVDMDKVQLHPTGLIDPKEPSNRTKILGPEALRGSGGILLNKQGRRFVNELDLRSVVSKAINAQKNEYPESD---GCFFAYCVLNEEASKLFGVNALDFYGKRLGLFQRVESVEELAH-LIGCDEAVLRDTLDNYEKCSSTKTTCPLTGKMVFPCALGAQGPYNVAF-VTPSIHYTMGGCLISPAAEILR--EHKGLNVLEDYRPIRGLFGAGEVTGGVHGGNRLGGNSLLECVVFGKIAGDRAATILQRRDAALSKTSWTSVVVRES-RSGEQFGVGSRVLRFNLPGALQRTGLSLGEFVAIRGEWDGQQLIGYYSPITLPDDLGTISLLVRADK--GTLKEWISALRPGDSVEVKACGGLRIDLDPVKKHLLFKGTPVIR-LGLISAGTGIAPMLQVIRAAL----------SKPYVDNVES-IRLIYAAEEYDTLTYRSILERFTQQYPDKFACDFVLNNPPEGWTGGVGFVDKKSLQKVLQPP---SSEPLIAICGPPPMQRDVTNELLG 1197          

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|1177789491|gb|ORC84661.1| (NADH-dependent fumarate reductase [Trypanosoma theileri])

HSP 1 Score: 775.393 bits (2001), Expect = 0.000e+0
Identity = 510/1289 (39.57%), Postives = 718/1289 (55.70%), Query Frame = 0

Query:   35 NTLLNLRPAPQLFNHPMTLFINPSKILHAMASLA----DGRAAASAVVA-PMNLRKPAAGPFTASAAGGWNDPALNMEEGELKKVLQTVTGTRGAMQYIAKV---GAPPERLDEIHA--LVEDVLKRAEQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQ-HLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVGDKLIH-HVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQ--ENGGSTTHVPAQKTVTGVTY--EDMATHEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAIGV-CLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLE---FTGSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEMVMCKMLGEIG 1303
            +T L LR     +N   +   +PS+ L+     +    DGR++AS V+  P+   K          +     PA      E ++V+ T+ G    + Y  ++   GA   +  E  A  ++++      +  + +  +SEV+++N+ P G+   +SE +  V+    ++Y  +   +DPA  PL +  +   LK+   G D+T  E++     +  ++   SF +     T+++ HD+A LDLGGL KG+ +D  V  L  AG  ++  EWGGD   +G++    PW++ V  PPSV+ ++          R E++                      K+V EL    +VL ++ A            L  +   D  ++ TSGDY  V +     +   +F  K K L+E     LAQ S+    S M ADALATA++      K  R  L+  + R     VT+Y  Y+RE  ++        E    R  R++   PARV+V GGGLAG SAAIEAA  GA+V+L+EKE+++GGNSAKATSGINGWGT  QA + + D  K FERDT  SG GG+C P L+R LS +S +A+ WL   +GV L  LSQLGGH+ KRTHRA   P G P PIG+ IM+ ++ ++R  L  D I I  +  V  L M E     +GG   H      VTGVTY   +    E  TL AD+V+L TGG+  D    SLL+E+ P L G+            P+TNG +ATGDGVKL   LGA  VDMDK+QLHPT  +DPKDP +PTK LGPEALRGSGGIL+N +G RFVNELDLRSVVS AI  Q   Y  ++   G+ +A+C+LN+ A + FG +AL +Y +  G+F+  +D   LA ++IGC    L  T+  Y  ++  G  CLKT K+ FP  +         A VTPSIHYTMGG  ISAAAE+L+  +    N+    RP+R LF AGEVTGGVHG NRLGGNSLLECVVFG++AG RA TI Q+R + L  E+WT V  RE  +++ ++G  + V RF+L GALQ++GL++GQF+AIRGE DG  L G+YSP++ P+E G + IL R D   G +  +++A+RPG    +KA  GLR++    T   IF+G   I+Q  L+AGGTG+APM+QI+RA L            P  +  T  +RL+YA+E   +L +  TLQ   ++ P  F    VLN PP GWT GVGF++  + ++ L  P ++    L  ICGPP  +  +   L  +G
Sbjct:   37 STTLALR-----WNSTTSRTSHPSQTLNYQQRFSSGGPDGRSSASMVIVDPVRAAKERDRMAREMLSSNLPRPA------EEERVVVTLKGLVHTVPYTLRIVEDGASLAKESEAKAKVILQECFDMVNKYLNHYNPESEVSKVNELPIGEKHKMSEHLLRVMECSVRVYTSSGTCFDPATGPLAKRLRDLMLKED--GDDITLSEEE-----VERFSLPRSFDVDLAQGTISRLHDEAKLDLGGLNKGYTVDCVVEKLNDAGIANVLFEWGGDCRASGVNYQRLPWAIAVTRPPSVNEVRE---------RAEAI-------------------AEGKEVEEL----SVLREKAA------------LLRVMYLDDEALCTSGDYENVVFNKKHGVRCSLFDWKKKGLLEPVDDELAQVSVKCY-SAMYADALATASVIKRDTVK-VRYMLE--TWRHSRNRVTNYAAYTREEERLAHMYEIARETPELRQERIAGSLPARVIVIGGGLAGLSAAIEAASCGAQVILMEKESKLGGNSAKATSGINGWGTRAQALKDIYDNCKTFERDTYLSGLGGMCAPGLVRALSVKSGDAIGWL-SSLGVPLTVLSQLGGHSRKRTHRAPDMPDGTPVPIGYTIMQTLERHIRNNL-SDHITIMENTSVKAL-MHETHGTPDGGHEVH------VTGVTYVKSNGEEKEQMTLTADSVILATGGFSNDREGKSLLREYAPHLFGT------------PTTNGPWATGDGVKLARRLGATLVDMDKIQLHPTGLIDPKDPASPTKYLGPEALRGSGGILLNNKGERFVNELDLRSVVSKAINMQDNEYPESK---GSRFAYCILNEAAAELFGRNALGYYWNVLGVFKRVEDEHELA-ELIGCSQETLHQTLVTYEATSRNGSRCLKTGKSVFPCVLG-PQGPFYVAFVTPSIHYTMGGCLISAAAEILQ--EHHSMNILEDQRPIRGLFGAGEVTGGVHGGNRLGGNSLLECVVFGKIAGDRAATILQKRDSALSTEKWTSVVVRESRRSE-QFGAGSRVLRFNLPGALQRSGLSLGQFIAIRGEWDGRQLIGYYSPITLPEERGTISILARGDK--GTLCEWISAMRPGDSVEIKACTGLRIDRDPNTKQIIFRG-HVIRQFGLIAGGTGVAPMLQIIRAAL----------ERPFVE-TTESIRLIYASEEYDELTYRSTLQDYAKSNPDKFACDFVLNSPPEGWTGGVGFIDRPSLQKTLQPPSDD---LLVAICGPPAMQRAVRNTLLNMG 1213          

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|340056975|emb|CCC51314.1| (putative NADH-dependent fumarate reductase [Trypanosoma vivax Y486])

HSP 1 Score: 773.852 bits (1997), Expect = 0.000e+0
Identity = 475/1167 (40.70%), Postives = 660/1167 (56.56%), Query Frame = 0

Query:  143 ALVEDVLKRAEQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVGDK---LIHHVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQENGGSTTHVPAQKTVTGVTYEDMATHEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAIGV-CLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLEFT--GSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEMVMCKMLGEIG 1303
            A++ D      Q  + +  +SEV+ +N    G    +S+ + +V+    ++Y  +  ++DPA  PL++ +++  + +L   D    +       + LY+   SF ++    T+++ H KA LDLGGL KG+ +D  V  LR AG +D+  EWGGD+H +G +  G+PW+V +  PPSV ++                                                 VL  +      GE E  P        D  ++ TSGDY  V  + D    ++  +F   +K+L+  S  GLAQ S+    + M ADALATA L +   P   R  LD    R     VT+Y+ Y+R+G +V        E    R +R+S   PARV+V GGGLAG SAAIEAA  GA+V+LLEKE ++GGNSAKATSGINGWGT  Q  + V D  KYFERDT +SG  G  DP+L++ LS +SA+A+ WL+  +GV L  LSQLGGH+  RTHRA  T  G P PIG+ IM+ ++ ++R+ L  D++ + T   V++L ++E +   G    V     V     E+    E   L ADAVVL TGG+  D ++ SLL+E+ P L               P+TNG++ATGDGVKL   LGA  VDMDK+QLHPT  VDP DP NPTK LGPEALRGSGGIL+N+ G+RFVNELDLRSVVSNAIL Q   Y G+    G+ +A+CVLN EA + FG+++L FY+   GLF+  ++   LA+ +IGCE   L+ T+E Y + +   V C  T K  +P  +       VA  VTPSIHYTMGG  IS AAEVL   + D  ++    RP+R LF AGEVTGGVHG NRLGGNSLLECVVFG++AG RA TI Q+    L   +WT V  RE  ++  ++G+ + V RF+L GA+Q++GL +G+FVAIRGE DG+ L G+YSP++ PD+ G + +L R D   G +  +++A+RPG    +KA GGL +E      ++    + I++  L+AGGTG+APM+QI+RA             +P      S VRL+YA+E    L +   L          F    VLN PP GWTEGVGF++  + +  L  P ++    L  ICGP V +  +   L ++G
Sbjct:  181 AVLTDAFDMVNQHLNHFNPNSEVSLVNDLAVGIKHQMSDHLRLVMECCIRVYRSSGELFDPATGPLIEFFRKSTR-NLECSDTIPCDD------LELYSLPRSFDVNLGDGTISRRHPKARLDLGGLNKGYTVDYVVERLRAAGLSDLMFEWGGDIHASGRNVNGQPWAVGIARPPSVESV-------------------------------------------------VLRAKEGRSATGEAETPPPFLCAVQLDNEAICTSGDYENV--INDPKHGVVCSIFDWGSKKLLSPSDGGLAQVSVKCYNA-MFADALATACL-VKRDPARVRQMLDGW--RFSRNCVTNYVAYTRQGERVAHMHEIAQESRELRRLRISGSLPARVIVVGGGLAGLSAAIEAAGCGAQVILLEKEKKLGGNSAKATSGINGWGTRAQLERDVQDGGKYFERDTYKSGLKGCTDPALVKTLSVKSADAINWLV-SLGVPLTVLSQLGGHSHMRTHRAPDTADGTPVPIGYTIMKTLENHIRKNL-KDRVTVMTGVSVIEL-LEETELMPGGRREVRVTGVVIARPGEN---EEPMKLPADAVVLATGGFSNDKTADSLLREYAPHL------------SNYPTTNGSWATGDGVKLARRLGAKLVDMDKIQLHPTGLVDPNDPANPTKFLGPEALRGSGGILLNKLGKRFVNELDLRSVVSNAILRQKNEYPGSN---GSYFAYCVLNAEATKLFGVNSLNFYEKQLGLFQRVENVTELAQ-LIGCEETVLQETLENYNECSTAKVRCPHTDKVVYPCALGPQGPYSVAF-VTPSIHYTMGGCLISPAAEVLR--EEDSFSILENHRPIRGLFGAGEVTGGVHGGNRLGGNSLLECVVFGKIAGDRAATILQKHEVALSRNDWTSVVVRET-RSGEQFGIGSCVLRFNLPGAVQRSGLKLGEFVAIRGEWDGQQLLGYYSPITLPDDLGTISLLVRGDK--GTLREWISALRPGDSVEIKACGGLCIEQDPENERLLFRNKVIRKFGLIAGGTGVAPMLQIIRAAF----------QKPYASTIDS-VRLIYASEDFESLTYRKILADYAEKNRDKFACRYVLNNPPEGWTEGVGFISRKSLQSTLQPPSDD---LLVAICGPQVMQRSVKTELLDMG 1243          

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|557864318|gb|ESS67586.1| (NADH-dependent fumarate reductase [Trypanosoma cruzi Dm28c])

HSP 1 Score: 768.459 bits (1983), Expect = 0.000e+0
Identity = 499/1252 (39.86%), Postives = 681/1252 (54.39%), Query Frame = 0

Query:   68 ADGRAAASAVVAPMNLRKPAAGPFTASAAGGWNDPALNMEEGELKKVLQTVTGTRGAMQYIAKVGAP-PERLDEIHALVEDVLKRA----EQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVGDKLIH-HVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKL--EMQEPQENGGSTTHVPAQKTVTGVTYEDMATHEIHT--LEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCK--PYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEY-AQSAAIGVCLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLEFTGSQ---IFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEMVMCKMLGEIG 1303
            ADGR++AS VV    L         A      N P+ + EE    +++ T+ G    + Y  ++    P        +   VLK A    +Q  + +  +SEV+ INQ P G    +S+ M  V+    ++Y  +   +DPA  PL++  +  +K      + T  E++     +  +    SF ++    T+ + H+ A LDLGG+ KG+ +D+ V  L  AG  D+  EWGGD    G++   +PW++ ++ PP V                                                    V  ++ A  GL E++   +L  +   D  ++ TSGDY  V +     +  ++F  K + L+E   S LAQ SI    S M ADALATA+L    + K  R  L+    R     VT+Y+ Y+R+G +V R     +E    R  R++   PARV+V GGGLAG SAAIEA   GA+V+LLEKE +VGGNSAKATSGINGWGT  QA   + D    FERDT +SG GG   PSL+R LS +S +A+ WL   +GV L  LSQLGGH+ KRTHRA     G P PIG+ IM  ++ +VR KL  D++ I  +  V  L  E++   + G        +  VTGVTY++    E  +  L ADAV+L TGG+  D  S SL+ E  P+L             G P+TNG +ATGDGVKL   LGA  VDMDKVQLHPT  +DPKDP NPTK LGPEALRGSGG+L+N++G RFVNELDLRSVVSNAI+ Q    PY G     G+ +A+CVLN  A + FG++ L FY +  G+F+   D   LA  +IGC+   L+ T+E Y + S A   C  T K  +P  +         A VTPSIHYTMGG  IS +AE+    +    NL    RP+  LF AGEVTGGVHG NRLGGNSLLECVVFGR+AG RA TI Q++   L +++WT V  RE  ++  ++G  + V RF+L GALQ++GL +GQF+AIRGE DG+ L G+YSP++ PDE G + IL R D   G +  +++A+RPG    +K+ GG+ +E   ++   +F G   I+Q  L+AGG+G+APM+QI+RA L            P  D  T  +RLVY AE   +L + + L    +  P  F     LN PP GWT GVGFV+  + R+ L  P   S   L  ICGPP  +  M   L  +G
Sbjct:   62 ADGRSSASIVVVDAELAAKERDRI-AREMLSQNMPSPHAEE----RLVVTMRGLEHTVPYTLRIVLNGPNEAKNGEVIAGKVLKEAFEVVDQHLNHYNPESEVSAINQLPLGVKHTMSQHMRRVMECCVRVYASSGACFDPATGPLVEFLRSVMKDEKSDAESTLTEEE-----VERFCLPQSFDVNMTDGTIARKHEGAKLDLGGVNKGYTVDRVVENLNAAGMRDVMFEWGGDCRATGVNYQHQPWAIAIVRPPPV----------------------------------------------------VQVEQRAKEGLDEKKEASQLLRLMYLDDEALCTSGDYENVMYSPKYGVRSNIFDWKKRSLLEPVESELAQVSIKCY-SAMYADALATASLIKREISK-VRHMLEEW--RHSRNRVTNYVTYTRQGERVARMFEIATENAEIRKNRIAGSLPARVIVVGGGLAGLSAAIEATACGAQVILLEKEPKVGGNSAKATSGINGWGTRAQALDDIQDNCNIFERDTHKSGLGGSTVPSLVRTLSVKSGDAISWL-SSLGVPLTVLSQLGGHSRKRTHRAPDKADGTPVPIGFTIMRTLEQHVRTKL-ADRVTIMENTLVTSLLHEIKGTPDGG-------REVRVTGVTYKNSDEKETSSMKLTADAVILATGGFSNDHMSQSLIGEFAPEL------------SGFPTTNGPWATGDGVKLARRLGATLVDMDKVQLHPTGLIDPKDPANPTKYLGPEALRGSGGVLLNKKGERFVNELDLRSVVSNAIIEQGDEYPYSG-----GSKFAFCVLNDAAVELFGVNLLNFYANTLGVFKRVDDLQGLAM-LIGCDVLTLQNTLEAYESNSIATSACPFTGKVVYPCVLG-PQGPFYVAFVTPSIHYTMGGCLISPSAEIQR--EHHSLNLLENQRPILGLFGAGEVTGGVHGGNRLGGNSLLECVVFGRIAGDRAATILQKQVYALSKDKWTSVVVRES-RSGERFGTGSRVLRFNLPGALQRSGLYLGQFIAIRGEWDGQQLIGYYSPITLPDERGVISILARGDK--GTLKEWISAMRPGDSVEIKSCGGILIERNPAKKQFLFHG-HVIRQFGLIAGGSGVAPMLQIIRAAL----------ERPFVD-TTESIRLVYTAEEYEELTYRELLNHYSKENPDKFSVEFSLNNPPEGWTGGVGFVDRPSLRKTLQPP---SNDLLIAICGPPAMQRTMKNDLLAMG 1199          

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|71747118|ref|XP_822614.1| (NADH-dependent fumarate reductase [Trypanosoma brucei brucei TREU927] >gi|788017012|ref|XP_011777633.1| NADH-dependent fumarate reductase, putative [Trypanosoma brucei gambiense DAL972] >gi|70832282|gb|EAN77786.1| NADH-dependent fumarate reductase, putative [Trypanosoma brucei brucei TREU927] >gi|261332374|emb|CBH15369.1| NADH-dependent fumarate reductase, putative [Trypanosoma brucei gambiense DAL972] >gi|1115870781|emb|SCU71016.1| NADH-dependent fumarate reductase [Trypanosoma equiperdum])

HSP 1 Score: 761.14 bits (1964), Expect = 0.000e+0
Identity = 472/1167 (40.45%), Postives = 659/1167 (56.47%), Query Frame = 0

Query:  140 EIHALVEDVLKRA----EQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVGDK-LIHHVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEA---RAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQENGGSTTHVPAQKTVTGVTYEDMA--THEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAIGV-CLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLE---FTGSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFE 1292
            E  A   ++L++A    +   + +  +SE++R+N  P G+   +SED+  V+     ++  +   +DPA  P++   +  ++      D++  E     A + L++   SF +  +  T+ + H +A LDLGG+ KG+ +D  V  LR AG  ++  EWGGD+  +G +  G  W+V +  PPSV               +E +RR                            A+  +          +++  P L ++   D  ++ TSGDY  V +     +   +F  + + L+      LAQ S+    S M ADALAT  L    V ++A   R  L+    R   + VT+Y  Y+R+G ++        E    R++R++   P+R+V+ GGGLAG SAAIEAA  GA+V+L+EKE R+GGNSAKATSGINGWGT  QA+  ++D  KYFERDT  SG GG  DP+L++VLS +S +A+ WL   +GV L  LSQLGGH+FKRTHRA     G P PIG  IM  ++ ++R  L  +++ I T   V +L  +      G++     +  VTGV Y D++    +   L ADAVVL TGG+  D    SLL ++ P L               P+TNG +ATGDGVKL  ++GA  VDMDKVQLHPT  +DPKDP N TKILGPEALRGSGGIL+N++G+RFVNELDLRSVVS AI TQ   Y G+    G  +A+CVLN++A   FG  AL FY    GLF+ A+    LAK +IGC+   L+ T+E+Y   +   V C  T K  FP  +  T      A VTPSIHYTMGG  IS AAEVL+  +    N+    RP+R LF AGEVTGGVHG NRLGGNSLLECVVFG++AG RA TI Q+R   L +  WT V  RE  ++  ++G  + V RF+L GALQ+ GL +G+FVAIRGE DG+ L G++SP++ P++ G + +L R D   G +  ++ A+RPG    +KA GGLR++        +F+  R I + +L+A GTG+APM+Q++RA L           +P  D   S +RL+YAAE    L +   LQ     +P  F    VLN PP GWT GVGFVN  + ++ L  P   S+  L V+CGPPV +
Sbjct:  138 EFEAKAAEILRKAFHMVDYSLNCFNPESELSRVNSLPVGEKHQMSEDLRHVMECTISVHHSSGMGFDPAAGPIISRLRGAMRDHNDMSDISVTE-----AEVELFSLAQSFDVDLEEGTIARKHSEARLDLGGVNKGYTVDYVVDHLRAAGMPNVLFEWGGDIRASGRNIKGNLWAVAIKRPPSV---------------EEVIRR----------------------------AKGKMLKMGEEEQEEKDDDSPSLLHVVELDDEALCTSGDYENVLYHPKHGVAGSIFDWQRRGLLSPEEGALAQVSVKCY-SAMYADALATVCL----VKRDAVRIRYLLEGW--RYVRSRVTNYFAYTRQGERLAHMHEIAQETRELREIRIAGSLPSRIVIVGGGLAGLSAAIEAASCGAQVILMEKEGRIGGNSAKATSGINGWGTRTQAKSDILDGGKYFERDTFLSGVGGTTDPALVKVLSVKSGDAIGWLT-SLGVPLSVLSQLGGHSFKRTHRAPDKTDGTPLPIGHTIMRTLEDHIRNNL-SERVTIMTHVSVTELLHETDTTPDGAS-----EVRVTGVRYRDLSDVDGQPSKLLADAVVLATGGFSNDREENSLLCKYAPHL------------ASFPTTNGPWATGDGVKLATSVGAKLVDMDKVQLHPTGLIDPKDPANTTKILGPEALRGSGGILLNKQGKRFVNELDLRSVVSKAINTQGNEYPGSG---GCYFAYCVLNEDATNLFGGGALGFYGKKLGLFQRAETVEELAK-LIGCDEGELRDTLEKYETCSKAKVACPVTGKVVFPC-VVGTRGPYNVAFVTPSIHYTMGGCLISPAAEVLQ--EYKGLNILENHRPIRCLFGAGEVTGGVHGGNRLGGNSLLECVVFGKIAGDRAATILQKREIALSKTSWTSVVVRES-RSGEQFGTGSRVLRFNLPGALQRTGLNLGEFVAIRGEWDGQQLVGYFSPITLPEDLGTISLLVRADK--GTLKEWICALRPGDSVEIKACGGLRIDQDPVKKCLLFR-NRPITRFALVAAGTGVAPMLQVIRAAL----------KKPYVDTLES-IRLIYAAEEYDTLTYRSILQRFAEEFPDKFVCNFVLNNPPEGWTGGVGFVNKKSLQKVLQPP---SSEPLIVVCGPPVMQ 1205          

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|71410008|ref|XP_807320.1| (NADH-dependent fumarate reductase [Trypanosoma cruzi strain CL Brener] >gi|70871295|gb|EAN85469.1| NADH-dependent fumarate reductase, putative [Trypanosoma cruzi])

HSP 1 Score: 759.985 bits (1961), Expect = 0.000e+0
Identity = 495/1252 (39.54%), Postives = 685/1252 (54.71%), Query Frame = 0

Query:   68 ADGRAAASAVVAPMNLRKPAAGPFTASAAGGWNDPALNMEEGELKKVLQTVTGTRGAMQYIAKV---GAPPERLDEIHA--LVEDVLKRAEQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVGDKLIH-HVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKL--EMQEPQENGGSTTHVPAQKTVTGVTYEDMATHEIHT--LEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCK--PYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAI-GVCLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLEFTGSQ---IFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFEMVMCKMLGEIG 1303
            ADGR++AS VV    L         A      N P+ + EE    +++ T+ G    + Y  ++   G    +  E+ A  ++++  +  +Q  + +  +SEV+ INQ P G    +S+ M  V+    ++Y  +   +DPA  PL++  +  +K      + T  E++     +  +    SF ++    T+ + H+ A LDLGG+ KG+ +D+ V  L  AG  D+  EWGGD    G++   +PW++ ++ PP V  ++                                   HAK+                  GL E++   +L  +   D  ++ TSGDY  V +     +  ++F  K + L+E   S LAQ SI    S M ADALATA+L    + K  R  L+    R     VT+Y+ Y+R+G +V R     +E    R  R++   PARV+V G GLAG SAAIEA   GA+V+LLEKE +VGGNSAKATSGINGWGT  QA   + D    FERDT +SG GG   PSL+R LS +S +A+ WL   +GV L  LSQLGGH+ KRTHRA     G P PIG+ IM  ++ +VR KL  D++ I  S  V  L  E++   + G        +  VTGVTY+     E  +  L ADAV+L TGG+  D  S SL+ E  P+L             G P+TNG +ATGDGVKL   LGA  VDM+KVQLHPT  +DPKDP NPTK LGPEALRGSGG+L+N++G RFVNEL+LRSVVSNAI+ Q    PY G     G+ +A+CVLN  A + FG++ L FY +  G+F+   D   LA  +IGC+   L+ T+E Y  S+ +   C  T K  +P  +         A VTPSIHYTMGG  IS +AE+    +    NL    RP+  LF AGEVTGGVHG NRLGGNSLLECVVFGR+AG RA TI Q++   L +++WT V  RE  ++  ++G  + V RF+L GALQ++GL +GQF+AIRGE DG+ L G+YSP++ PDE G + IL R D   G +  +++A+RPG    +K+ GG+ +E   ++   +F G   I+Q  L+AGG+G+APM+QI+RA L            P  D  T  +RLVY AE   +L + + L    +  P  F     LN PP GWT GVGFV+  + R+ L  P   S   L  ICGPP  +  M   L  +G
Sbjct:   61 ADGRSSASIVVVDAELAAKERDRI-AREMLSQNMPSPHAEE----RLVVTMRGLEHTVPYTLRIVLNGPNEAKNGEVIAGKVLKEAFEVVDQHLNHYNPESEVSAINQLPLGAKHTMSQHMRRVMECCVRVYASSGACFDPATGPLVEFLRSVMKDEKSDAESTLTEEE-----VERFCLPQSFDVNMTDGTIARKHEGAKLDLGGVNKGYTVDRVVEKLNAAGMRDVMFEWGGDCRATGVNYQHQPWAIAIVRPPPVEVVEQ----------------------------------HAKE------------------GLDEKKEASQLLRLMYLDDEALCTSGDYENVMYSPKYGVRSNIFDWKKRSLLEPVESELAQVSIKCY-SAMYADALATASLIKRDISK-VRHMLEEW--RHSRNRVTNYVTYTRQGERVARMFEIATENAEIRKNRIAGSLPARVIVVGCGLAGLSAAIEATACGAQVILLEKEPKVGGNSAKATSGINGWGTRAQALDDIQDNCNIFERDTHKSGLGGSTVPSLVRTLSVKSGDAISWL-SSLGVPLTVLSQLGGHSRKRTHRAPDKADGTPVPIGFTIMRTLEQHVRTKL-ADRVTIMESTVVTSLLNEIKGTPDGG-------REVRVTGVTYKKSDEKEAGSMKLTADAVILATGGFSNDHMSQSLIGEFAPEL------------SGFPTTNGPWATGDGVKLARRLGATLVDMEKVQLHPTGLIDPKDPANPTKYLGPEALRGSGGVLLNKKGERFVNELELRSVVSNAIIEQGDEYPYSG-----GSKFAFCVLNDAAVKLFGVNLLNFYANTLGVFKRVDDLQGLAM-LIGCDVLTLQNTLETYESSSIVTSACPFTGKVVYPC-VVGPQGPFYVAFVTPSIHYTMGGCLISPSAEIQR--EHYSLNLLENQRPILGLFGAGEVTGGVHGGNRLGGNSLLECVVFGRIAGDRAATILQKQVYALSKDKWTSVVVRES-RSGERFGTGSRVLRFNLPGALQRSGLYLGQFIAIRGEWDGQQLIGYYSPITLPDERGVISILARGDK--GTLKEWISAMRPGDSVEIKSCGGILIERNPAKKQFLFHG-HVIRQFGLIAGGSGVAPMLQIIRAAL----------ERPYVD-TTESIRLVYTAEEYEELTYRELLHHYSKENPDKFSVEFSLNNPPEGWTGGVGFVDRPSLRKTLQPP---SNDLLIAICGPPAMQRAMKNDLLAMG 1198          

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|401429196|ref|XP_003879080.1| (NADH-dependent fumarate reductase-like protein [Leishmania mexicana MHOM/GT/2001/U1103] >gi|322495330|emb|CBZ30634.1| NADH-dependent fumarate reductase-like protein [Leishmania mexicana MHOM/GT/2001/U1103])

HSP 1 Score: 759.599 bits (1960), Expect = 0.000e+0
Identity = 479/1160 (41.29%), Postives = 665/1160 (57.33%), Query Frame = 0

Query:  141 IHALVEDVLKRAEQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVG--DKLIHHVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEARAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQ-EPQENGGSTTHVPAQKTVTGVTYEDM--ATHEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYA-QSAAIGVCLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLEFT--GSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFE 1292
            + A++ D     ++  + +  DSEV+++N+ P G+   +S+ +  V+   +++Y+++   +DPA +PL+      L+ +    D T+ +    +     +    SF +     T+ + H++A LDLGGL KG+ +D  V  L  A F D+  EWGGD   +G++   +PW+V V+ PPSV  +         L   +S + +T             ++ H+      + A++     +A+ G  + E R  L  + L + A + TSGDY  V +     + +   +    + L+E   +GLAQ ++    SC+ ADALATA+  +   P   R  L+    R     VTDY  Y+REG ++        E    R  R++   PARVVV GGGLAG +AAIEA   GA V+LLEKE R+GGNSAKATSGINGWGT  QA   V+D  K+FERDT  SG+GG CDP L+R LS +SAEA+ WL +  G+ L  L QLGG + +R HRA     G P P+G+ IM  ++ Y+R KL   ++ I     VV L        +G    H      V GV Y+ +  A+  +  L ADAVVL TGG+  D ++ SLL+E+ P++ G+            P+TNG FATGDGVK+   LGA  VDMDKVQLHPT  +DPKDP N TK LGPEALRGSGGIL+N+ G RFVNELDLRSVVS AI+ Q   Y  +    G+ +A+CVL++EA   FG S+L +Y  ++GLF    D   LA ++IGC   +L  T+E Y  QS    VC  T K  FP  +  T      A VTPSIHYTMGG  IS AAE+L   Q    N++  +RP+  LF AGEVTGGVHG NRLGGNSLLECVVFG++AG RA TI Q+    L +++W PV  RE   +D ++GV + V RF+L GA Q +GL VG+F+ IRG+ DG+ L G+YSP++ PD++G + IL R D   G +  +++++RPG    +KA GGLR+E      Q+   K  I++L L+AGG+G+APM+QI++A L          N P  D     +RLVYAAE E +L +   L+  +   P  F    VLN PP GWTEGVG+V+  T +  L  P   S   L  ICGPPV +
Sbjct:   88 VKAILSDCFAMVDKHLNSFNPDSEVSQVNRMPVGEKHVMSKHLFEVVKCCEEVYNISGSCFDPAAAPLVHK----LRDAACRHDSTAEDFAITSEEAGRFTLTSSFAIDIKEGTIARKHEEAMLDLGGLNKGYTVDCVVDQLNAANFADVLFEWGGDCRASGVNAQRQPWAVGVVRPPSVDEI---------LAAAKSGKSVT-------------MNAHSLGDHTDESAQST----SAADGAAKAEQRALLRVMSLSNEA-LCTSGDYENVLFASALGRALSSTYNWSRRCLIEPCQNGLAQVTVKCY-SCLYADALATASF-VKRDPVRVRYILEHY--RHDYNRVTDYAAYTREGERLAHMYEIAHESPACRIERIAGSLPARVVVIGGGLAGCAAAIEAVSCGATVILLEKEPRLGGNSAKATSGINGWGTRTQAVNHVLDNCKFFERDTFLSGEGGHCDPGLVRTLSVKSAEAISWL-ESFGIPLTVLYQLGGASRRRCHRAPDQKDGTPVPVGFTIMRHLEDYIRTKL-QGKVTILNEMEVVSLMHDVSAMPDGNREIH------VHGVRYKSVTDASGTVMDLLADAVVLATGGFSNDRTTNSLLREYAPNVYGT------------PTTNGTFATGDGVKMARKLGATLVDMDKVQLHPTGLIDPKDPSNRTKYLGPEALRGSGGILLNKNGERFVNELDLRSVVSQAIIAQDNEYPDSG---GSKFAYCVLSEEAATLFGKSSLTYYWKSQGLFTRVDDMKALA-ELIGCSVESLHRTLETYERQSTVKKVCPLTGKLVFP-SVVGTKGPYYVAYVTPSIHYTMGGCFISPAAELLMEDQ--SVNIFEDMRPILGLFGAGEVTGGVHGGNRLGGNSLLECVVFGKIAGDRAATILQKEKYGLSKDKWVPVVVRESRASD-QFGVGSRVLRFNLPGATQTSGLTVGEFIGIRGDWDGQQLIGYYSPINMPDDKGRISILARGDK--GNLQEWISSMRPGDSVEMKACGGLRIELKPHQKQMVYRKTVIRKLGLIAGGSGVAPMLQIIKAAL----------NRPYVD-GIETIRLVYAAEDEHELTYRSLLKQYRADNPDKFDCGFVLNNPPEGWTEGVGYVDRATLQSLLPPP---SKGLLVAICGPPVMQ 1168          

BLAST of EWM27529.1 vs. NCBI_GenBank
Match: gi|60460036|gb|AAX20162.1| (mitochondrial NADH-dependent fumarate reductase [Trypanosoma brucei])

HSP 1 Score: 758.059 bits (1956), Expect = 0.000e+0
Identity = 471/1167 (40.36%), Postives = 658/1167 (56.38%), Query Frame = 0

Query:  140 EIHALVEDVLKRA----EQVFSLWIKDSEVNRINQAPRGQAIDLSEDMSVVLLAVDKLYDVTKGVYDPACSPLLQHYKQHLKQSLRGFDVTSVEQDPQAAAIALYAHWGSFILSKDGKTLTKAHDKAGLDLGGLAKGWAIDQCVAALRRAGFNDIFMEWGGDVHVAGMHPTGRPWSVRVMEPPSVHTLQNTVPTPAALGRQESLRRMTDSSGMGPVPRSPLLSGHAKDVFELQEAEAVLADRTASLGLGEEEARPELANIPLRDGASVTTSGDYMQVFWVGDK-LIHHVFQSKTKRLMEVSSSGLAQASIVTEGSCMLADALATAALAMGAVPKEA---RAFLDAVSPRLGGTTVTDYLLYSREGPKVLRHRVRGSEGDVARDVRLSQHEPARVVVAGGGLAGFSAAIEAAKTGARVVLLEKEARVGGNSAKATSGINGWGTLPQARQGVMDEEKYFERDTCRSGKGGLCDPSLIRVLSTQSAEAVRWLMDDMGVDLDELSQLGGHAFKRTHRARPTPSGDPRPIGWLIMERVQAYVREKLPPDQIEIRTSARVVKLEMQEPQENGGSTTHVPAQKTVTGVTYEDMA--THEIHTLEADAVVLTTGGYGCDLSSTSLLKEHRPDLLGSAHGGNGSSDLGMPSTNGAFATGDGVKLGLALGALTVDMDKVQLHPTSFVDPKDPVNPTKILGPEALRGSGGILINQEGRRFVNELDLRSVVSNAILTQCKPYVGAEGQVGNVYAWCVLNKEAQQNFGLSALRFYKDARGLFEAAKDFPTLAKDVIGCEPAALKATIEEYAQSAAIGV-CLKTRKNAFPVPIAWTDTDLVAARVTPSIHYTMGGLRISAAAEVLEGAQMDRGNLYRKLRPVRRLFAAGEVTGGVHGANRLGGNSLLECVVFGRLAGGRAGTIKQRRPTCLEEEEWTPVTFREVLQTDFKYGVNTMVFRFHLHGALQQAGLAVGQFVAIRGELDGEWLQGFYSPLSRPDEEGYVEILCRTDTAGGQIINFLNAVRPGGECYLKAMGGLRLE---FTGSQIFQGKREIKQLSLLAGGTGIAPMIQIVRAYLYHLVGAGAPENEPLPDPATSGVRLVYAAEREFDLAFTDTLQSIQRAYPAYFRHYVVLNKPPLGWTEGVGFVNADTARRHLYYPYEESAHQLFVICGPPVFE 1292
            E  A   ++L++A    +   + +  +SE++R+N  P G+   +SED+  V+     ++  +   +DPA  P++   +  ++      D++  E     A + L++   SF +  +  T+ + H +A LDLGG+ KG+ +D  V  LR AG  ++  EWGGD+  +G +  G  W+V +  PPSV               +E +RR                            A+  +          +++  P L ++   D  ++ TSGDY  V +     +   +F  + + L+      LAQ S+    S M ADALAT  L    V ++A   R  L+    R   + VT+Y  Y+R+G ++        E    R++R++   P+R+V+ GGGLAG SAAIEAA  GA+V+L+EKE R+GGNSAKATSGINGWGT  QA+  ++D  KYFERDT  SG GG  DP+L++VLS +S +A+ WL   +GV L  LSQLGGH+FKRTHRA     G P PIG  IM  ++ ++R  L  +++ I T   V +L  +      G++     +  VTGV Y D++    +   L ADAVVL TGG+  D    SLL ++ P L               P+TNG +ATGDGVKL  ++GA  VDMDKVQLHPT  +DPKDP N TKILGPEALRGSGGIL+N++G+RFVNELDLRSVVS AI TQ   Y G+    G  +A+CVLN++A   F   AL FY    GLF+ A+    LAK +IGC+   L+ T+E+Y   +   V C  T K  FP  +  T      A VTPSIHYTMGG  IS AAEVL+  +    N+    RP+R LF AGEVTGGVHG NRLGGNSLLECVVFG++AG RA TI Q+R   L +  WT V  RE  ++  ++G  + V RF+L GALQ+ GL +G+FVAIRGE DG+ L G++SP++ P++ G + +L R D   G +  ++ A+RPG    +KA GGLR++        +F+  R I + +L+A GTG+APM+Q++RA L           +P  D   S +RL+YAAE    L +   LQ     +P  F    VLN PP GWT GVGFVN  + ++ L  P   S+  L V+CGPPV +
Sbjct:  138 EFEAKAAEILRKAFHMVDYSLNCFNPESELSRVNSLPVGEKHQMSEDLRHVMECTISVHHSSGMGFDPAAGPIISRLRGAMRDHNDMSDISVTE-----AEVELFSLAQSFDVDLEEGTIARKHSEARLDLGGVNKGYTVDYVVDHLRAAGMPNVLFEWGGDIRASGRNIKGNLWAVAIKRPPSV---------------EEVIRR----------------------------AKGKMLKMGEEEQEEKDDDSPSLLHVVELDDEALCTSGDYENVLYHPKHGVAGSIFDWQRRGLLSPEEGALAQVSVKCY-SAMYADALATVCL----VKRDAVRIRYLLEGW--RYVRSRVTNYFAYTRQGERLAHMHEIAQETRELREIRIAGSLPSRIVIVGGGLAGLSAAIEAASCGAQVILMEKEGRIGGNSAKATSGINGWGTRTQAKSDILDGGKYFERDTFLSGVGGTTDPALVKVLSVKSGDAIGWLT-SLGVPLSVLSQLGGHSFKRTHRAPDKTDGTPLPIGHTIMRTLEDHIRNNL-SERVTIMTHVSVTELLHETDTTPDGAS-----EVRVTGVRYRDLSDVDGQPSKLLADAVVLATGGFSNDREENSLLCKYAPHL------------ASFPTTNGPWATGDGVKLATSVGAKLVDMDKVQLHPTGLIDPKDPANTTKILGPEALRGSGGILLNKQGKRFVNELDLRSVVSKAINTQGNEYPGSG---GCYFAYCVLNEDATNLFCGGALGFYGKKLGLFQRAETVEELAK-LIGCDEGELRDTLEKYETCSKAKVACPVTGKVVFPC-VVGTRGPYNVAFVTPSIHYTMGGCLISPAAEVLQ--EYKGLNILENHRPIRCLFGAGEVTGGVHGGNRLGGNSLLECVVFGKIAGDRAATILQKREIALSKTSWTSVVVRES-RSGEQFGTGSRVLRFNLPGALQRTGLNLGEFVAIRGEWDGQQLVGYFSPITLPEDLGTISLLVRADK--GTLKEWICALRPGDSVEIKACGGLRIDQDPVKKCLLFR-NRPITRFALVAAGTGVAPMLQVIRAAL----------KKPYVDTLES-IRLIYAAEEYDTLTYRSILQRFAEEFPDKFVCNFVLNNPPEGWTGGVGFVNKKSLQKVLQPP---SSEPLIVVCGPPVMQ 1205