EWM28374.1, cds2297 (CDS) Nannochloropsis gaditana

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|585110744|gb|EWM28374.1| (n -(beta-n-acetylglucosaminyl)-l-asparaginase [Nannochloropsis gaditana])

HSP 1 Score: 878.626 bits (2269), Expect = 0.000e+0
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query:    1 MLPVCSLRLSYSQKFLFFVVVPPFVPSVTGIRRLTAPCYMQHIILVLFFCFVSFGHPFSHNLISTGHSDLSDMTAGMPHPKGAVVAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVVWKEGLGKAWVEEADDTGTA 432
            MLPVCSLRLSYSQKFLFFVVVPPFVPSVTGIRRLTAPCYMQHIILVLFFCFVSFGHPFSHNLISTGHSDLSDMTAGMPHPKGAVVAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVVWKEGLGKAWVEEADDTGTA
Sbjct:    1 MLPVCSLRLSYSQKFLFFVVVPPFVPSVTGIRRLTAPCYMQHIILVLFFCFVSFGHPFSHNLISTGHSDLSDMTAGMPHPKGAVVAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVVWKEGLGKAWVEEADDTGTA 432          

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|553184851|ref|XP_005854156.1| (asparaginase [Nannochloropsis gaditana CCMP526] >gi|422294908|gb|EKU22208.1| asparaginase [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 724.931 bits (1870), Expect = 0.000e+0
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 0

Query:   73 MTAGMPHPKGAVVAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVVWKEGLGKAWVEEADDTGTA 432
            MTAGMPHPKGAVVAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVVWKEGLGKAWVEEADDTGTA
Sbjct:    1 MTAGMPHPKGAVVAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVVWKEGLGKAWVEEADDTGTA 360          

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|299469827|emb|CBN76681.1| (Asparaginase, c-terminal [Ectocarpus siliculosus])

HSP 1 Score: 238.424 bits (607), Expect = 6.414e-73
Identity = 134/262 (51.15%), Postives = 167/262 (63.74%), Query Frame = 0

Query:  169 MCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIRE---GGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVVWKEGLGKAWVEEAD 427
            M G+  +YG V A++GV +A S AR VME S HS+L G GA+TFA   G E  E L+  A++++  W+  +  E    D R E                 HDTVG++ LD  GNL AGTSTSGW FKHPGRVGD+P+VGSGLY DS+VG AVATGDGEEI+R CLSF VVE MR G  P LAC+ GI RL+  ++    G    E   MH+ LTVAVMA++ +G +GAASTLG  N HRGRP FP  +W++G     VEE +
Sbjct:    1 MEGTSMSYGAVLAVRGVVKAFSAARVVMERSPHSVLAGEGATTFAVRNGIEAAETLTDDAKQQFEDWR-RQHLEEEGSDHRGES----------------HDTVGVICLDHDGNLCAGTSTSGWKFKHPGRVGDAPVVGSGLYCDSSVGAAVATGDGEEILRTCLSFAVVEFMRGGDSPQLACKKGIARLEETVKRSAAGRSVEEVNRMHDRLTVAVMAMNPKGDIGAASTLGPRNVHRGRPSFPLAIWRKGDPVRVVEETE 245          

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|1126524079|ref|WP_075162415.1| (hypothetical protein [Chthonomonas calidirosea] >gi|946700473|emb|CEK14004.1| asparaginase [Chthonomonas calidirosea])

HSP 1 Score: 218.009 bits (554), Expect = 4.259e-64
Identity = 139/314 (44.27%), Postives = 178/314 (56.69%), Query Frame = 0

Query:   84 VVAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNE-GKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAAST 396
            ++  TW FG+ A  K   +L    +                    ALDA+EA  NVVE D  +   VGYGG PNAEG++ELDAAIM G++ T G VAAL G  R ISVAR VME + H +LVG  A  FA   GF  +E+L+ AAR+ WL W+ N+ G E    D   +E                HDT+GL  LD +GNLAAG +TSG  +K PGRVGDSPIVGSGLY D+ +G A ATGDG+EIM+VCLS+  V  +  G     AC+   + L+ L+R+   E  R         A++AL K+G  GAA+T
Sbjct:    4 LILATWRFGQAACAKGFSLLAEGLS--------------------ALDAIEAAANVVEED-PNVNSVGYGGLPNAEGDVELDAAIMDGAQHTAGAVAALTGFRRPISVARCVMERTPHVMLVGENAKRFALQAGFVEEELLTEAARQRWLAWRSNQTGAEVAHFDRLSKEG---------------HDTIGLCALDKNGNLAAGCTTSGLAWKLPGRVGDSPIVGSGLYVDNELGAAAATGDGDEIMKVCLSYRAVMLLERGYTAQQACE---EALRYLLRKRPPETCR-----PYGAALIALRKDGDCGAAAT 273          

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|551644229|ref|XP_005824906.1| (hypothetical protein GUITHDRAFT_77590 [Guillardia theta CCMP2712] >gi|428168988|gb|EKX37926.1| hypothetical protein GUITHDRAFT_77590 [Guillardia theta CCMP2712])

HSP 1 Score: 214.542 bits (545), Expect = 1.075e-63
Identity = 119/251 (47.41%), Postives = 154/251 (61.35%), Query Frame = 0

Query:  169 MCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRL---KVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVVWKE 416
            M G     G V A+QG+S  +SVAR++ME   H++L G GA TFA   GF+ + +L+  +R E+ +WK             +E++  V       D  A HDT+G++  D  G L   TSTSGW FK PGRVGDSP+VGSG Y D A G AVATGDGEEIMR CLSFLVVE+MR G  P  AC+  I RL   K  +     +GE +  H+ LTV ++A++  G VGA+STLG  N HRG+P FP +VWK+
Sbjct:    1 MRGRGYRLGCVLAVQGISTPVSVARKLMEHGQHTVLAGEGAKTFALENGFKEENILTDESRREFELWK-------------REQNFTV-------DKEASHDTIGIICRDETGELVVATSTSGWKFKLPGRVGDSPLVGSGFYVDQACGAAVATGDGEEIMRGCLSFLVVERMRMGDSPTRACEHAIARLRSKKEFLSGASFQGENR-QHKKLTVGIVAMNTNGDVGASSTLGPANIHRGKPAFPCIVWKQ 230          

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|512723833|ref|WP_016481858.1| (asparaginase [Chthonomonas calidirosea] >gi|510022277|emb|CCW34296.1| Asparaginase [Chthonomonas calidirosea T49] >gi|946703353|emb|CEK15184.1| asparaginase [Chthonomonas calidirosea])

HSP 1 Score: 216.468 bits (550), Expect = 1.339e-63
Identity = 138/314 (43.95%), Postives = 178/314 (56.69%), Query Frame = 0

Query:   84 VVAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNE-GKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAAST 396
            ++  TW FG+ A  K   +L    +                    ALDA+EA  NVVE ++ +   VGYGG PNAEG +ELDAAIM G++ T G VAAL G  R ISVAR VME + H +LVG  A  FA   GF  +E+L+ AAR+ WL W+ N+ G E    D   +E                HDT+GL  LD +GNLAAG +TSG  +K PGRVGDSPIVGSGLY D+ +G A ATGDG+EIM+VCLS+  V  +  G     AC+   + L+ L+R+   E  R         A++AL K+G  GAA+T
Sbjct:    4 LILATWRFGQAACAKGFSLLAEGLS--------------------ALDAIEAAANVVE-EDPNVNSVGYGGLPNAEGVVELDAAIMDGAQHTAGAVAALTGFRRPISVARCVMERTPHVMLVGENAKRFALQAGFVEEELLTEAARQRWLAWRSNQTGAEVAHFDRLSKEG---------------HDTIGLCALDKNGNLAAGCTTSGLAWKLPGRVGDSPIVGSGLYVDNELGAAAATGDGDEIMKVCLSYRAVMLLERGYTAQQACE---EALRYLLRKRPPETCR-----PYGAALIALRKDGDCGAAAT 273          

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|1126526792|ref|WP_075165126.1| (hypothetical protein [Chthonomonas calidirosea] >gi|944157522|emb|CEK14005.1| asparaginase [Chthonomonas calidirosea])

HSP 1 Score: 216.083 bits (549), Expect = 2.413e-63
Identity = 131/269 (48.70%), Postives = 166/269 (61.71%), Query Frame = 0

Query:  129 ALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNE-GKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAAST 396
            ALDA+EA  NVVE ++ +   VGYGG PNAEG +ELDAAIM G++ T G VAAL G  R ISVAR VME + H +LVG  A  FA   GF  +E+L+ AAR+ WL W+ N+ G E    D   +E                HDT+GL  LD +GNLAAG +TSG  +K PGRVGDSPIVGSGLY D+ +G A ATGDG+EIM+VCLS+  V  +  G     AC+   + L+ L+R+   E  R         A++AL K+G  GAA+T
Sbjct:   29 ALDAIEAAANVVE-EDPNVNSVGYGGLPNAEGVVELDAAIMDGAQHTAGAVAALTGFRRPISVARCVMERTPHVMLVGENAKRFALQAGFVEEELLTEAARQRWLAWRSNQTGAEVAHFDRLSKEG---------------HDTIGLCALDKNGNLAAGCTTSGLAWKLPGRVGDSPIVGSGLYVDNELGAAAATGDGDEIMKVCLSYRAVMLLERGYTAQQACE---EALRYLLRKRPPETCR-----PYGAALIALRKDGDCGAAAT 273          

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|1200467142|gb|OUV82466.1| (glycosylasparaginase [Verrucomicrobiaceae bacterium TMED137])

HSP 1 Score: 204.527 bits (519), Expect = 5.772e-59
Identity = 137/345 (39.71%), Postives = 179/345 (51.88%), Query Frame = 0

Query:   80 PKGAVVAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIMCGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIWKGNEGKEGPQVDGRKEESAGVPAP-PREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVVWKEGLGKAWV 423
            P   ++  TW FG+   +KA + L    +                     LDAVE GI VVE D +++  VG  G PNA G ++ DA IM GS    G VAA++G+   I+ ARRVME++ H +LVG GA  FA  +G E  +V S    +EW                 +++S  +P   P++      HDTV LLVLD+ G++A G STSGW  K PGRVGDSPI+GSGLY D+ VG A ATG GE +MR C SF+VVE MR+GL P  AC   IQR+  +  +           E L +  +ALDK GR GAA +          P F  V+    L K  V
Sbjct:   14 PGEPLIVSTWPFGKAGNDKALDTLKHKGS--------------------LLDAVEQGIRVVESDEKNRS-VGITGLPNAAGVVQQDACIMNGSDHNAGSVAAIEGILHPITAARRVMEKTPHVMLVGDGARKFAIREGLESIKVDSQKLHKEW----------------HQKQSKPIPNKRPKDN-----HDTVALLVLDSKGHIAGGCSTSGWGGKLPGRVGDSPIIGSGLYVDNKVGAAGATGLGENVMRYCASFMVVEYMRKGLHPEEACVKTIQRIAAIDPKSA---------EDLHLNFVALDKRGRFGAAGSGSGFRYSVTTPNFSKVLEGSALSKKDV 307          

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|516518980|ref|WP_017907346.1| (N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase [Xanthomonas sp. SHU199])

HSP 1 Score: 204.527 bits (519), Expect = 2.129e-58
Identity = 139/343 (40.52%), Postives = 186/343 (54.23%), Query Frame = 0

Query:   78 PHPKGAV-VAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIM-----CGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIW-KGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVV 413
            P P+GA  V  TW FG  A + A +VL                         ALDAVEAG+ V E D  D + VG GG+P+ +G + LDA IM     CGS      VAAL+ +  AISVARRVME++ H +LVG GA  FA SQGFE  ++L+PA+ + W  W K +       ++ R      +P           HDT+G+L LDAHGNL+   +TSG  +K  GRVGDSPI+G+GLY D+ VGGA +TG GEE++R   SF VVE MR+G  PA AC+  + R   ++R       +  +   L V  +A++K G VGA +          +PGF + V
Sbjct:   42 PLPRGAARVISTWDFGVAANQAAWQVLSRGGA--------------------ALDAVEAGVRVPEAD-PDNHTVGLGGYPDRDGRVTLDACIMDHTGGCGS------VAALEDIVHAISVARRVMEKTPHVMLVGDGALQFALSQGFERTKLLTPASEQAWKEWLKTSHYAPEANIENRAYRRGTLPGGKDN------HDTIGMLALDAHGNLSGACTTSGMAWKMHGRVGDSPIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMR---IVR------RKPELTRDLQVGFLAMNKRGEVGAFAI---------QPGFSYAV 333          

BLAST of EWM28374.1 vs. NCBI_GenBank
Match: gi|959939146|ref|WP_058195948.1| (N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase [Xanthomonas translucens])

HSP 1 Score: 203.756 bits (517), Expect = 2.934e-58
Identity = 136/343 (39.65%), Postives = 187/343 (54.52%), Query Frame = 0

Query:   78 PHPKGAV-VAGTWLFGRLAVEKAAEVLLSSSTSPTAPPSIDHPSETNLATSIALDAVEAGINVVELDNQDQYYVGYGGFPNAEGEMELDAAIM-----CGSRKTYGGVAALQGVSRAISVARRVMEESVHSLLVGRGASTFARSQGFEWDEVLSPAAREEWLIW-KGNEGKEGPQVDGRKEESAGVPAPPREGDAVAPHDTVGLLVLDAHGNLAAGTSTSGWCFKHPGRVGDSPIVGSGLYADSAVGGAVATGDGEEIMRVCLSFLVVEKMREGLGPALACQAGIQRLKVLIREGGGEGERQGMHESLTVAVMALDKEGRVGAASTLGEHNRHRGRPGFPFVV 413
            P P+GA  V  TW FG  A + A +VL                         ALDAVEAG+ V E D  D   VG GG+P+ +G + LDA IM     CGS      VAAL+ +  AISVARRVME++ H +LVG GA  FA +QGFE   +L+P++ + W  W K ++      ++ R  +   +P           HDT+G+L LDAHGNL+   +TSG  +K  GRVGDSPI+G+GLY D+ VGGA +TG GEE++R   SF VVE+MR+G  PA AC+  + R   ++R       +  +   L V  +A+++ G VGA +          +PGF + V
Sbjct:   37 PLPRGAARVISTWDFGVAANQAAWQVLARGGA--------------------ALDAVEAGVKVPEAD-PDNPTVGLGGYPDRDGRVTLDACIMDHTGGCGS------VAALEDIVHAISVARRVMEKTPHVMLVGDGALQFALAQGFERTRLLTPSSEKAWKAWLKTSKYAPEANIENRAYQQGTLPGGKDN------HDTIGMLALDAHGNLSGACTTSGMAWKMHGRVGDSPIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVERMRQGKSPAEACREVVMR---IVR------RKPQLTRDLQVGFLAMNRRGEVGAFAI---------QPGFSYAV 328