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Overview
Mutinfo:NoDGTT5, diacylglycerol acyltransferase type 2–5 |
Organism:Nannochloropsis oceanica (N. oceanica CCMP1779) |
Method:Overexpression |
Genes:jgi.p|Nanoce1779_2|545914 |
Reporter:Yellow FP (Venus variant) |
Promoter:EF |
Selection:Hygromycin |
Phenotypes:Yeast expressing NoDGTT5 accumulated TAG to the highest level. Over-expression of NoDGTT5 in CCMP1779 grown in N-replete medium resulted in levels of TAG normally observed only after N deprivation. Reduced growth rates accompanied NoDGTT5 over-expression in CCMP1779. Constitutive expression of NoDGTT5 in Arabidopsis thaliana was accompanied by increased TAG content in seeds and leaves. A broad substrate specificity for NoDGTT5 was revealed, with preference for unsaturated acyl groups. Furthermore, NoDGTT5 was able to successfully rescue the Arabidopsis tag1-1 mutant by restoring the TAG content in seeds.
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Publications: |
- Zienkiewicz K, Zienkiewicz A, Poliner E, Du ZY, Vollheyde K, Herrfurth C, Marmon S, Farré EM, Feussner I, Benning C. Nannochloropsis, a rich source of diacylglycerol acyltransferases for engineering of triacylglycerol content in different hosts. Biotechnology for biofuels. 2017; 10:8.
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