malonyl CoA-acyl carrier protein transacylase

Overview


Mutinfo: malonyl CoA-acyl carrier protein transacylase
Organism: Nannochloropsis oceanica (N. oceanica IMET1)
Method: Overexpression
Genes: NO07G02760
Reporter: Flag tag
Promoter: Hsp20
Selection: Zeocin
Phenotypes: NoMCAT overexpressing N. oceanica showed a higher growth rate and photosynthetic efficiency. The neutral lipid content of engineered lines showed a significant increase by up to 31% compared to wild type. Gas chromatography-mass spectrometry analysis revealed that NoMCAT overexpression significantly altered the fatty acid composition.
Publications:
Chen JW, Liu WJ, Hu DX, Wang X, Balamurugan S, Alimujiang A, Yang WD, Liu JS, Li HY. Identification of a malonyl CoA-acyl carrier protein transacylase and its regulatory role in fatty acid biosynthesis in oleaginous microalga Nannochloropsis oceanica. Biotechnology and applied biochemistry. 2017 Sep; 64(5):620-626.