EWM20990.1, cds9681 (CDS) Nannochloropsis gaditana

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|585100986|gb|EWM20990.1| (microcystin synthetase-associated thioesterase [Nannochloropsis gaditana])

HSP 1 Score: 696.427 bits (1796), Expect = 0.000e+0
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0

Query:    1 MRLICLNDVLFVPSLSFPSHRLTSSYIERERSQDPMGCGSSCCIPPYVSKAIEVHAAAEPKKASDAPTDSQSTFKAGPRRPVMSEAMAQKWLLSNGKPVAGAKVRIICLGWAGANSNVFKTWDLKCGEVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLHRWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWLGKVIEE 334
            MRLICLNDVLFVPSLSFPSHRLTSSYIERERSQDPMGCGSSCCIPPYVSKAIEVHAAAEPKKASDAPTDSQSTFKAGPRRPVMSEAMAQKWLLSNGKPVAGAKVRIICLGWAGANSNVFKTWDLKCGEVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLHRWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWLGKVIEE
Sbjct:    1 MRLICLNDVLFVPSLSFPSHRLTSSYIERERSQDPMGCGSSCCIPPYVSKAIEVHAAAEPKKASDAPTDSQSTFKAGPRRPVMSEAMAQKWLLSNGKPVAGAKVRIICLGWAGANSNVFKTWDLKCGEVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLHRWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWLGKVIEE 334          

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|585112398|gb|EWM29817.1| (microcystin synthetase-associated thioesterase [Nannochloropsis gaditana])

HSP 1 Score: 225.328 bits (573), Expect = 3.697e-67
Identity = 138/340 (40.59%), Postives = 195/340 (57.35%), Query Frame = 0

Query:   36 MGCGSS-----CCIP--------PYVSKAIEVHAA--AEPKKASDAPTDSQSTFKA--------------------------GPRRPV-MSEAMAQKWLLSNGKPVAGAKVRIICLGWAGANSNVFKTWDLKCGEVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLH-RWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWLGKVI 332
            MGC SS     CC+P        P V  A E+     A P   +  PT SQ   +A                            R P  M E  A+KWL    K  A  +VR++C  +AG NSN +KTWD+K  E+V VELP RNAR+N+K I DV ++AKKI+ A   +GY+ES  PLV FGHSFGA++   +A+I++ +Y+YTPK  +  GC PLH R G P  +   +DDE++ S++D GG+TKEMA+++ +V+  L  +K D+L +D+YV++E A  L+ P+  +G   D R P  +  EW R+TT   + + FPGGHFFLK  E +V+A++  VI
Sbjct:   41 MGCTSSKGCCLCCVPDAALKSCGPAVPIAKEITNGYPAPPGHGTHTPTHSQGLGEAIQTRTRCISACAVRCIHDHLPGHRHDHQRAPAKMREEEARKWLSVKRKDFA--RVRLVCFPYAGGNSNFYKTWDVKEAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRP-KFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHE-APPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSVI 376          

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|585101141|gb|EWM21085.1| (thioesterase [Nannochloropsis gaditana])

HSP 1 Score: 152.14 bits (383), Expect = 6.473e-40
Identity = 90/268 (33.58%), Postives = 138/268 (51.49%), Query Frame = 0

Query:   88 AQKWLLSNGKPVAGAKVRIICLGWAGANSNVFKTWDLKCG-EVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVI--------------FGHSFGAMIGLHLARIMKKEYDYTPK-----MCIFGGCRPLHRWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCS--IQIKTFPGGHFFLKEKEADVLAWLGKVIE 333
            A++W +    P   AK R++CL WAG NS++++TWDL    EVV VELP RNAR  + P+ + ++L  ++  A  V+GYL S+ P  +              FGHSFGAM+  HLA+ +++  +   K     + +  G  PL + G         DD MIQ +V  GG+  E+A  + L++  L   + D+  +D YV+      L   L    G +D R+  E +  W + TT      +  FPGGHFF+KE  + VL  +G+ + 
Sbjct:   57 AKRWFVCRA-PRPEAKYRMLCLAWAGGNSSIYRTWDLGSEVEVVAVELPGRNARVKETPLKNFHLLVSRLLHAMEVVGYLPSSAPSFLPSSTSPPPLKPLLLFGHSFGAMLVTHLAQALRERREDEGKSVDVPLVLVSGMAPLDKRGGSTGVSAMSDDAMIQYLVKMGGIPSEVAASRELMNVFLPAFRGDYTAIDNYVYT-PLPALPFRLVAFAGENDARASKEIMEGWVKMTTHEKDFAVDVFPGGHFFIKEHTSMVLERIGQRVR 322          

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|504968554|ref|WP_015155656.1| (thioesterase [Chroococcidiopsis thermalis] >gi|428010549|gb|AFY89112.1| Oleoyl-(acyl-carrier-protein) hydrolase [Chroococcidiopsis thermalis PCC 7203])

HSP 1 Score: 130.568 bits (327), Expect = 2.015e-32
Identity = 70/239 (29.29%), Postives = 124/239 (51.88%), Query Frame = 0

Query:   97 KPVAGAKVRIICLGWAGANSNVFKTWDLKCG---EVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLHRWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWLGKVI 332
            +P   AK+R+ C  +AG ++ VF+TW+       EV  ++LP R +R  + P   ++ L + +  A F   YL+   P V FGHS GA+IG  LAR  ++    +P      G +  H    P+  +   + E +Q +    G  KE+ ++  ++  ++  ++ D +  + YV+  +  +LE P+ + GGL+DP +  EQL  W  +T  +  I+ FPG HFF+   +  +L  + +V+
Sbjct:   15 QPNPQAKLRLFCFPYAGGSATVFRTWNRDLPSFVEVCPIQLPGRGSRLRETPFTQISPLVQTLGNALF--PYLDK--PYVFFGHSLGALIGFELARYFRRNISSSPIHLFVSGRQAPHIPD-PLPRHALPEQEFLQEIKCLNGTPKEVLENTEMMQLLIPLLRADLVLDEAYVYTSEP-RLECPITVFGGLEDPETNSEQLEAWREHTNTAFSIEMFPGNHFFINTAQPLLLQAIAQVL 247          

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|1052324123|emb|SCE89538.1| (medium-chain acyl-[acyl-carrier-protein] hydrolase [Micromonospora chaiyaphumensis])

HSP 1 Score: 130.183 bits (326), Expect = 3.660e-32
Identity = 76/236 (32.20%), Postives = 115/236 (48.73%), Query Frame = 0

Query:   97 KPVAGAKVRIICLGWAGANSNVFKTWDLKCG---EVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLHRWGFPM-SYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWL 328
            +P+ GA +R+IC  +AG  ++VF +W    G   EV  V+LP R +R   +P  DV  L   +     + G L+   P  +FGHS GA++    AR +       P   +F   RP      P    +   DD++I  +   GG   ++  D  L+  VL  ++ D    D YV +     L+ P+  +GG DDP +PP  L  W R+TT   ++  F GGHF+L+E   D+L  L
Sbjct:   24 RPLPGAALRLICFPYAGGGASVFASWPAGTGPDIEVCAVQLPGRESRLGQRPFQDVRTLVSAVEAG--LGGLLDE--PFALFGHSMGALVAFEFARRLAARGRPAPAH-LFVSARPAPHLPRPWPRAHLLPDDQLIDRIRQIGGTPAQVLADARLMRLVLPVLRADFAVNDGYV-HRPGPLLDCPVTALGGRDDPEAPPRVLEAWARHTTGPFRLHRFDGGHFYLREHRDDLLRVL 253          

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|299116155|emb|CBN76062.1| (fatty acid-ACP thioesterase [Ectocarpus siliculosus])

HSP 1 Score: 130.183 bits (326), Expect = 3.816e-32
Identity = 80/271 (29.52%), Postives = 132/271 (48.71%), Query Frame = 0

Query:   83 MSEAMAQKWLLSNGKPVAGAKVRIICLGWAGANSNVFKTWDLKCGEVVKV---ELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTP-----------KMCIFGGCRPLHRWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVF----NEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLK-EKEADVLAWLGKVIEE 334
            MS +  +KW+    +  A A +R+ C  WAG +++ F  W  K  E V++   ELP R  R  +  +  V+ + + ++QA   MG L+   P  +FGHSFG+++   LAR +K++    P            MC+ GG  P      P+S    D   ++  ++  GGM +E+     LV   L  I+ D+   + Y +     + +  +  P+  +GG +D R+P   L  W   T    +++ F GGHFFL+ E E  VL +LG ++ E
Sbjct:    1 MSSSALEKWVACR-RVKADASIRLFCAAWAGGSASSFNKWTSKLPEAVELHALELPGRMRRSKEDSLTSVSAIVEGVAQALVTMGLLDK--PFALFGHSFGSLVMFELARKLKEDAGKIPVYLIVSGCCPPDMCVGGGSSP------PISQLPRD--ALVDHLIGLGGMPEELRDQTELVDYFLPPIRADYTAYEGYRYELRPGDDSRPITCPIICLGGEEDGRAPRSDLERWSSLTAAEFRLQMFGGGHFFLQGEGEQAVLTFLGDILTE 260          

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|501063191|ref|WP_012114475.1| (thioesterase [Xanthobacter autotrophicus] >gi|154160482|gb|ABS67698.1| Thioesterase [Xanthobacter autotrophicus Py2])

HSP 1 Score: 129.413 bits (324), Expect = 4.054e-32
Identity = 69/232 (29.74%), Postives = 119/232 (51.29%), Query Frame = 0

Query:  104 VRIICLGWAGANSNVFKTWDLKCGEVVKV---ELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLHRWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWLGKVI 332
            +R++CL  AGA++ +F+ W +    +  V   ELP R  R+      D+  LA  ++         + T PLV++GHS GA++   +AR + ++      + + G   P H  G P + +   D E++  +   GG +KE+     L++  L  ++ D   +D Y F +    L  P  ++GG  D  +PPE L  W    +  + ++ +PGGHFF++++EA +LA L KVI
Sbjct:    1 MRLVCLPHAGASAALFRPWKVTLSGIATVDSPELPGRGTRYGAPFATDMAALADDLADTLA-----QRTVPLVLYGHSMGALLAFEVARSLARQGVSVAGLVLSGRAAP-HLDGPPRARHTMTDAELVAELTAIGGTSKEVLAQPDLLAMTLPAVRADFRMVDTYAF-KPGPALAVPAIILGGSQDAATPPETLHAWSALISGPLTVELWPGGHFFVRDQEARLLALLAKVI 225          

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|929862306|ref|WP_054096230.1| (thioesterase [Thermoactinomyces vulgaris] >gi|928414757|gb|KPC72520.1| hypothetical protein ADL26_14325 [Thermoactinomyces vulgaris])

HSP 1 Score: 127.872 bits (320), Expect = 1.694e-31
Identity = 73/235 (31.06%), Postives = 123/235 (52.34%), Query Frame = 0

Query:   97 KPVAGAKVRIICLGWAGANSNVFKTWDLKCG---EVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLHRWGFPMSY---YKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVL 325
            KP   A++R+ C  +AG ++++F  W  +     EV  V+LP R  R  ++P+  +  L  +++    +  YL+   P +IFGHS GA+IG  LAR +++     P+  I    +  H    P S    Y   D+E+I  + +  G  +E+  ++ L+  +L  I+ D   +DRYV+ E+ E L  P+   GG++DP    E+L  W + TT    ++ F GGHF+L ++  D L
Sbjct:   13 KPNPQAELRLFCFPYAGGSASIFTQWQARFSNRIEVCPVQLPGRENRLLEEPVSSIERLVPQLADE--LEPYLDQ--PFLIFGHSMGALIGFELARELRRRNRPLPQKLIVSAKQAPH---IPRSKPLGYDFSDEELINQLRELNGTPEEVLANRELMELILPIIRADFALVDRYVYREE-EPLACPILAFGGIEDPDVKQEELAAWQQMTTGGFVMQQFAGGHFYLFQENTDAL 239          

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|1082692586|gb|OGA14913.1| (putative thioesterase [Betaproteobacteria bacterium RIFCSPLOWO2_02_FULL_63_19] >gi|1082730314|gb|OGA49639.1| putative thioesterase [Betaproteobacteria bacterium RIFCSPLOWO2_12_FULL_63_13])

HSP 1 Score: 123.25 bits (308), Expect = 1.478e-29
Identity = 71/240 (29.58%), Postives = 115/240 (47.92%), Query Frame = 0

Query:   98 PVAGAKVRIICLGWAGANSNVFKTWDLKCG---EVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLHRWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWLGKVIEE 334
            P   A++R+ C  +AG  ++VF++W        EV  V+LP R  R  + P  D+  LA+ +  A  +  YL+   P  I+GHS GA++   L RI++++    P+       R  H        Y   D E IQ++   GG+  E+ KD+ L+  +L  ++ D    DRY ++     L  P  L  G DD  +PPE +  W  ++  S  +  F GGHFFL+     ++  +   +E 
Sbjct:   16 PNPAARLRLFCFPFAGGGASVFRSWARALPPTIEVCPVQLPGRENRLRESPYTDLRPLAEVL--AGQIQWYLQK--PFAIYGHSMGALLAFELVRILQRQNAPLPRTLFLAAHRAAHLSPRRSPLYALPDKEFIQALHRFGGLQDEVMKDQELLDVLLPTLRADLTLCDRYSYS-PGSPLNCPFQLYVGRDDQEAPPEDMESWREHSAQSSSLCIFSGGHFFLRSDGDALMQAIATELER 250          

BLAST of EWM20990.1 vs. NCBI_GenBank
Match: gi|1086583572|emb|SDM25793.1| (Surfactin synthase thioesterase subunit [Dendrosporobacter quercicolus])

HSP 1 Score: 122.865 bits (307), Expect = 1.914e-29
Identity = 72/260 (27.69%), Postives = 132/260 (50.77%), Query Frame = 0

Query:   84 SEAMAQKWLLSNGKPVAGAKVRIICLGWAGANSNVFKTWDLKCGE---VVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCR--------PLHRWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWLGKVI 332
            +++    WLL+  +P A   +R+ CL +AG  ++V++ W  +  E   +  ++LP R  R  + P  D+  L  +IS+A  ++ YL+   P ++FGHS GA +   +AR ++++++  P   I  G R        PLH  G P       D   I+ +    G  + + + + L+   L  ++ D    + YV++   + LE P+   GG  DP +  +++  W R+T+CS  ++   GGHFFL+     +L  +GK++
Sbjct:    3 NQSTVTPWLLT--RPDARDIIRVFCLPYAGGGASVYRGWAKELPEGAGIYPIQLPGRENRIAEPPARDMTTLVHEISRA--IVPYLQQ--PFILFGHSLGARLAFEIARNIRRKWNLQPSRLIVSGSRAPEIPEPKPLH--GLP-------DSAFIEELRRFSGTPEAVLQSRELMELFLPVLRADFTVDETYVYSAD-KPLECPISAFGGTKDPEAGSKEVQGWARHTSCSFSLEMLEGGHFFLQTARPALLRSVGKIL 246