EWM21396.1, cds9277 (CDS) Nannochloropsis gaditana
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Overview
Properties
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Analyses
This CDS is derived from or has results from the following analyses
Annotated Terms
The following terms have been associated with this CDS:
Homology
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|585101614|gb|EWM21396.1| (Tetratricopeptide-like helical [Nannochloropsis gaditana]) HSP 1 Score: 2391.3 bits (6196), Expect = 0.000e+0 Identity = 1167/1167 (100.00%), Postives = 1167/1167 (100.00%), Query Frame = 0
Query: 1 MGNRVEVSQIVAADDSKWRRRRRRGRGGNQVGPSRLPVTRYSYRVATAMLVTGLFILTSSGPGGSRVHAFVLPVSFSSSTVSSSPHLLSAGALRGTHPHTRSPPASLLAASLISKDQDSYLSGEGASPSCFSPPPPSTPSSARPPSRPPLPIRLPPRPQPFQEPYSQQHPYQAPPRSPHSFRSSTRNATRWIEAAHINGTALPPQDYLLLKDLVRRREAMQARAFCFSLLEGSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYVFTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEGEGVQGGNREEHGRQGRDGQALIRSERWVDGPVDRAGSPGGQKRKRAPPPLSTGRTRAKTILTRMEREVRRAQVVAGAEKRPGETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGYAARGNAAATNALVREMHRKGVEPNVFTFINLVRACTLAGCPEKALSVVDPAFSWDFYSAAITIYHQSGCFAEADALYQQAVLHGKVPVDVTLTPADHALDLHGMSAPVAAAAVRYALKRIREEWWRCGPAAVKDLVLISGVGKGSLVPFRPTLRPAVQDMLVEQYYPPIDSTTQPGNAGRVVVGRESLLAWLEGKEGGGQGEGEGGGEDQERDVAEESMS 1167
MGNRVEVSQIVAADDSKWRRRRRRGRGGNQVGPSRLPVTRYSYRVATAMLVTGLFILTSSGPGGSRVHAFVLPVSFSSSTVSSSPHLLSAGALRGTHPHTRSPPASLLAASLISKDQDSYLSGEGASPSCFSPPPPSTPSSARPPSRPPLPIRLPPRPQPFQEPYSQQHPYQAPPRSPHSFRSSTRNATRWIEAAHINGTALPPQDYLLLKDLVRRREAMQARAFCFSLLEGSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYVFTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEGEGVQGGNREEHGRQGRDGQALIRSERWVDGPVDRAGSPGGQKRKRAPPPLSTGRTRAKTILTRMEREVRRAQVVAGAEKRPGETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGYAARGNAAATNALVREMHRKGVEPNVFTFINLVRACTLAGCPEKALSVVDPAFSWDFYSAAITIYHQSGCFAEADALYQQAVLHGKVPVDVTLTPADHALDLHGMSAPVAAAAVRYALKRIREEWWRCGPAAVKDLVLISGVGKGSLVPFRPTLRPAVQDMLVEQYYPPIDSTTQPGNAGRVVVGRESLLAWLEGKEGGGQGEGEGGGEDQERDVAEESMS
Sbjct: 1 MGNRVEVSQIVAADDSKWRRRRRRGRGGNQVGPSRLPVTRYSYRVATAMLVTGLFILTSSGPGGSRVHAFVLPVSFSSSTVSSSPHLLSAGALRGTHPHTRSPPASLLAASLISKDQDSYLSGEGASPSCFSPPPPSTPSSARPPSRPPLPIRLPPRPQPFQEPYSQQHPYQAPPRSPHSFRSSTRNATRWIEAAHINGTALPPQDYLLLKDLVRRREAMQARAFCFSLLEGSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYVFTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEGEGVQGGNREEHGRQGRDGQALIRSERWVDGPVDRAGSPGGQKRKRAPPPLSTGRTRAKTILTRMEREVRRAQVVAGAEKRPGETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGYAARGNAAATNALVREMHRKGVEPNVFTFINLVRACTLAGCPEKALSVVDPAFSWDFYSAAITIYHQSGCFAEADALYQQAVLHGKVPVDVTLTPADHALDLHGMSAPVAAAAVRYALKRIREEWWRCGPAAVKDLVLISGVGKGSLVPFRPTLRPAVQDMLVEQYYPPIDSTTQPGNAGRVVVGRESLLAWLEGKEGGGQGEGEGGGEDQERDVAEESMS 1167
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|553180823|ref|XP_005853467.1| (putative udp-glucosyltransferase, partial [Nannochloropsis gaditana CCMP526] >gi|422295594|gb|EKU22893.1| putative udp-glucosyltransferase, partial [Nannochloropsis gaditana CCMP526]) HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0 Identity = 290/291 (99.66%), Postives = 290/291 (99.66%), Query Frame = 0
Query: 861 MEREVRRAQVVAGAEKRPGETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGYAARGNAAATNALVREMHRKGVEPNVFTFINLVRACTLAGCPEKALSVVDPAFSWDFYSAAITIYHQSGCFAEADALYQQAVLHGKVPVDVTLTPADHALDLHGMSAPVAAAAVRYALKRIREEWWRCGPAAVKDLVLISGVGKGSLVPFRPTLRPAVQDMLVEQYYPPIDSTTQPGNAGRVVVGRESLLAWLEGKEGGGQGEGE 1151
MEREVRRAQVVAGAEKR GETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGYAARGNAAATNALVREMHRKGVEPNVFTFINLVRACTLAGCPEKALSVVDPAFSWDFYSAAITIYHQSGCFAEADALYQQAVLHGKVPVDVTLTPADHALDLHGMSAPVAAAAVRYALKRIREEWWRCGPAAVKDLVLISGVGKGSLVPFRPTLRPAVQDMLVEQYYPPIDSTTQPGNAGRVVVGRESLLAWLEGKEGGGQGEGE
Sbjct: 1 MEREVRRAQVVAGAEKRSGETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGYAARGNAAATNALVREMHRKGVEPNVFTFINLVRACTLAGCPEKALSVVDPAFSWDFYSAAITIYHQSGCFAEADALYQQAVLHGKVPVDVTLTPADHALDLHGMSAPVAAAAVRYALKRIREEWWRCGPAAVKDLVLISGVGKGSLVPFRPTLRPAVQDMLVEQYYPPIDSTTQPGNAGRVVVGRESLLAWLEGKEGGGQGEGE 291
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|551540996|ref|XP_005758827.1| (hypothetical protein EMIHUDRAFT_53066, partial [Emiliania huxleyi CCMP1516] >gi|485606664|gb|EOD06398.1| hypothetical protein EMIHUDRAFT_53066, partial [Emiliania huxleyi CCMP1516]) HSP 1 Score: 283.493 bits (724), Expect = 1.284e-81 Identity = 161/427 (37.70%), Postives = 235/427 (55.04%), Query Frame = 0
Query: 307 PSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTI 732
P++ ++ L ++ R+K W SL +M GV+PN +Y ++ W + L +L A N + + A+ + A +L++EM + + PD ++Y+AAISACE++ C ALSL+ M G PN+Y+Y+AAISAC + GR +EA L EM + GLRPN+V Y+ +SACE+ G D A LL EM AG+ +VI++N IS C++ G + AL LR+M V+PD IS+NAAI+AC + G E LL M + V D +Y+ + +A + L +L +M G R +PDVISY A+S+ EK G A LL M AG+ N+Y+Y ++ CEK GR +AL LL M GLTP+ Y + AC A + L+LL +MQ DGV P +
Sbjct: 8 PNVFSYSILINLCARSKDWKTAVSLLREMPNAGVQPNVISYNAAISVCEKASQWKKALSLLREMPGAGVQPNVISYNAAISACEKAGRCDEALSLLREMPD-VGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPGAG-VQPDVISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSLLREM--PGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLSPNEYSYNAAISACEKAGRCNEALSLLREMPGAGLTPNVISYNAAISACEKAGRCDEALSLLSKMQEDGVQPDV 430 HSP 2 Score: 243.047 bits (619), Expect = 4.412e-67 Identity = 145/426 (34.04%), Postives = 223/426 (52.35%), Query Frame = 0
Query: 373 ASCASNYVFTMALKGLKVR-RNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQ---VVVGKLEEEG 794
A C N L L R ++WK A +L++EM + P+ ++Y AAIS CE++S ALSL+ M G PN +Y+AAISAC + GR DEA L EM G++P+++ Y+ +SACE+ G D A LL EM AG+ + ++N IS C++ G WE A+ LR+M + P+ +S++AAI+AC + G +E LL M + + +YN + +AG + L +L +M G +PDVISY A+SA EK G A LL M AG++ + +Y+ ++ CEK R +AL LL M + PD Y + +C A + L+LL M + G+ P + Y + A G+ A+ L +M + PN + +A I A + G ++ K++E+G
Sbjct: 4 AGCEPNVFSYSILINLCARSKDWKTAVSLLREM-PNAGVQPNVISYNAAISVCEKASQWKKALSLLREMPGAGVQPNVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAG-LRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREM--PGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLSPNEYSYNAAISACEKAGRCNEALSLLREMPGAGLTPNVISYNAAISACEKAGRCDEALSLLSKMQEDG 425 HSP 3 Score: 202.986 bits (515), Expect = 3.954e-53 Identity = 119/353 (33.71%), Postives = 180/353 (50.99%), Query Frame = 0
Query: 440 MEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEE 792
M G PN ++YS I+ C R A L EM + G++PN++ YN +S CE+ A LL EM AG+ +VI++N IS C++ G + AL LR+M + V+PD IS++AAI+AC + G +E LL M V + +YN + +AG + + + +L +M G RP+V+SY+ A+SA EK G + A LL M AG+ N +Y ++ CEK GR +AL LL M G+ PD Y + AC A + L+LL M + GV P + Y+ + A + A+ L +M RV P+ + +A I + + G + L E
Sbjct: 1 MRQAGCEPNVFSYSILINLCARSKDWKTAVSLLREMPNAGVQPNVISYNAAISVCEKASQWKKALSLLREMPGAGVQPNVISYNAAISACEKAGRCDEALSLLREMPDVG-VQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAG--LRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLRE 350 HSP 4 Score: 174.866 bits (442), Expect = 2.058e-43 Identity = 128/466 (27.47%), Postives = 208/466 (44.64%), Query Frame = 0
Query: 510 MRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEGEGVQGGNREEHGRQGRDGQALIRSERWVDGPVDRAGSPGGQKRKRAPPPLSTGRTRAKTILTRMEREVRRAQVVAGAEKRPGETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGYAARGNAAATNALVREMHRKGVEPNVFTF 975
MR AG + +V +++ LI++C +W+ A+ LR+M V+P+ IS+NAAI+ C + ++ LL M V + +YN + +AG + L +L +M G +PDVISY+ A+SA EK G + A LL M AG++ N+Y+Y ++ CEK GRW +A+ LL M GL P+ Y+ + AC A + L+LL M + G+ P + Y + A G+ A+ L +M V P+ + +A I A + G + L E GVQ D A +K R LS R R++ +V + ++ G + S R +P + G+ + Y+ + A + G A +L+ M L P+V +YN + G +L+ +M GV+P+V +F
Sbjct: 1 MRQAGCEPNVFSYSILINLCARSKDWKTAVSLLREMPNAG-VQPNVISYNAAISVCEKASQWKKALSLLREMPGAGVQPNVISYNAAISACEKAGRCDEALSLLREMPDVG--VQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLRE--MPSAGVQP-----------------------DVISYSAAISACEKASRCDEALSLLREMPG---ARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDA--GLSPNEYSYNAAISACEKAGRCNEALSLLREMPGAGLTPNVISYNAAISACEKAGRCDEALSLLSKMQEDGVQPDVISF 433 HSP 5 Score: 162.54 bits (410), Expect = 2.566e-39 Identity = 124/431 (28.77%), Postives = 198/431 (45.94%), Query Frame = 0
Query: 232 GSRPLSVHSYLTLMKNLGRAGDWEGAVGIFE----------------ALRRRHQGVGGKEFMKLIRVLARAG------KYEAAMAYWDKA----EALGL--------GPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQ----------------------------TLDR------VEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTY 593
G P +V SY L+ R+ DW+ AV + A+ + K+ + L+R + AG Y AA++ +KA EAL L P ++ ++ +A + SL +M GV+PN+ +Y + + W E + +L A N V ++ A+ + A +L++EM + ++P+ ++Y AAISACE++ C ALSL+ M G P+ +Y+AAISAC + GR +EA L EM S G++P+++ Y+ +SACE+ D A LL EM A + DVI++N IS C++ G AL LR+M +L R + P+ IS+NAAI+AC + G +E LL +M+ V D ++
Sbjct: 5 GCEP-NVFSYSILINLCARSKDWKTAVSLLREMPNAGVQPNVISYNAAISVCEKASQWKKALSLLREMPGAGVQPNVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDA-GLSPNVISYNAAISACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRGNEALSLLREMPSAGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLSPNEYSYNAAISACEKAGRCNEALSLLREMPGAGLTPNVISYNAAISACEKAGRCDEALSLLSKMQEDGVQPDVISF 433
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|551550884|ref|XP_005762936.1| (hypothetical protein EMIHUDRAFT_52144, partial [Emiliania huxleyi CCMP1516] >gi|485612117|gb|EOD10507.1| hypothetical protein EMIHUDRAFT_52144, partial [Emiliania huxleyi CCMP1516]) HSP 1 Score: 276.559 bits (706), Expect = 2.224e-78 Identity = 167/500 (33.40%), Postives = 260/500 (52.00%), Query Frame = 0
Query: 276 FMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASN-YVFTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVI 774
+ +LI AR G A D+ G P + ++ L ++ R+K W SL +M GV PN+ +Y + A + W + + +L A + Y + A+ + A +L EM + PD ++Y A ISACE++ ALSL+ M G P+ +YSAAISAC + R +EA L+ EM + G+RPN++ +N +SACE+ G D A L +EM AAG+ DVI++N IS C++ G+WE+AL LR+M + PD IS++AAI+AC + E L M A V + ++N + +AG + L +L +M G PDVISY+ A+SA EK G +E A LL M AG+ N+++Y ++ CEK R +AL L M G+ P+ ++ + AC A + L+LL++M+ DGV P + Y+ + A + A+ L+ + L R V P+ + +AV+
Sbjct: 1 WTQLISAHARVGDCRRAFGLLDEMRQAGC-EPDVFSYSILINLCARSKDWKTAVSLLREMPGAGVPPNEVSYSAAISACAKAGRWQKAVSLLREMPGAGVQPDVYSYNAAISACEKAGRCDEALSLFDEMPAA-GVQPDVISYNATISACEKAGQWEKALSLLREMPGAGLSPDVISYSAAISACEKASRCNEALSLFDEMLAAGVRPNVISFNATISACEKAGRCDEALSLFDEMPAAGVQPDVISYNATISACEKGGQWEKALSLLREMPGAG-LSPDVISYSAAISACEKASRCNEALSLFDEMLAAGVRPNVISFNATISACEKAGRCDEALLLLREMPDAG--LSPDVISYSAAISACEKAGQWEKALSLLREMPAAGVPPNEFSYNATISACEKASRCNEALSLFDEMLAAGVRPNVISFSAAISACEKAGRCDDALSLLQKMREDGVQPNVISYSAAIQACAAAQQPDEALRLFKEAL-RSVEPDRITFNAVL 494 HSP 2 Score: 251.136 bits (640), Expect = 3.052e-69 Identity = 153/433 (35.33%), Postives = 224/433 (51.73%), Query Frame = 0
Query: 366 ILDAATLASCASNYVFTMALKGLKVR-RNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEG 797
+LD A C + L L R ++WK A +L++EM + P+ V+Y+AAISAC ++ A+SL+ M G P+ Y+Y+AAISAC + GR DEA L+ EM + G++P+++ YN +SACE+ G + A LL EM AG+ DVI+++ IS C++ AL +M V P+ ISFNA I+AC + G +E L M A V D +YN + + G + L +L +M G PDVISY+ A+SA EK A L M AG+R N ++ ++ CEK GR +AL LL M GL+PD Y+ + AC A + L+LL M GV P Y + A + A+ L+ +MLA V PN + SA I A + G + L++ ++G
Sbjct: 20 LLDEMRQAGCEPDVFSYSILINLCARSKDWKTAVSLLREMPGA-GVPPNEVSYSAAISACAKAGRWQKAVSLLREMPGAGVQPDVYSYNAAISACEKAGRCDEALSLFDEMPAAGVQPDVISYNATISACEKAGQWEKALSLLREMPGAGLSPDVISYSAAISACEKASRCNEALSLFDEMLAAG-VRPNVISFNATISACEKAGRCDEALSLFDEMPAAGVQPDVISYNATISACEKGGQWEKALSLLREM--PGAGLSPDVISYSAAISACEKASRCNEALSLFDEMLAAGVRPNVISFNATISACEKAGRCDEALLLLREMPDAGLSPDVISYSAAISACEKAGQWEKALSLLREMPAAGVPPNEFSYNATISACEKASRCNEALSLFDEMLAAGVRPNVISFSAAISACEKAGRCDDALSLLQKMREDG 448 HSP 3 Score: 164.851 bits (416), Expect = 1.613e-39 Identity = 139/511 (27.20%), Postives = 218/511 (42.66%), Query Frame = 0
Query: 487 YNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEGEGVQGGNREEHGRQGRDGQALIRSERWVDGPVDRAGSPGGQKRKRAPPPLSTGRTRAKTILTRMEREVRRAQVVAGAEKRPGETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGY--AARGNAAATNALVREMHRKGVEPNVFTFINLVRACTLAGCPEKALSVV 995
+ ++SA R GD AF LL+EMR AG + DV +++ LI++C +W+ A+ LR+M V P+ +S++AAI+ACA+ G ++ LL M G +PDV SY A+SA EK G + A L M AG++ + +Y ++ CEK G+W +AL LL M GL+PD Y+ + AC A + L+L + M GV P + + + A G+ A+ L+ +M A V P+ + +A I A +GG + + L E G G+ D A +K R LS + + + V A ++ G ++ R +P + G+ D Y + A + G A +L+ M +PP+ +YN + A+R N A + L EM GV PNV +F + AC AG + ALS++
Sbjct: 1 WTQLISAHARVGDCRRAFGLLDEMRQAGCEPDVFSYSILINLCARSKDWKTAVSLLREMPGAG-VPPNEVSYSAAISACAKAGRWQKAVSLLREMP-------------------------------------GAGVQPDVYSYNAAISACEKAGRCDEALSLFDEMPAAGVQPDVISYNATISACEKAGQWEKALSLLREMPGAGLSPDVISYSAAISACEKASRCNEALSLFDEMLAAGVRPNVISFNATISACEKAGRCDEALSLFDEMPAAGVQPDVISYNATISACEKGGQWEKALSLLRE--MPGAGLSP-----------------------DVISYSAAISACEKASRCNEALSLFD---EMLAAGVRPNVISFNATISACEKAGRCDEALLLLREMPDA--GLSPDVISYSAAISACEKAGQWEKALSLLREMPAAGVPPNEFSYNATISACEKASRCNEALS--LFDEMLAAGVRPNVISFSAAISACEKAGRCDDALSLL 441
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|551550926|ref|XP_005762957.1| (hypothetical protein EMIHUDRAFT_52395, partial [Emiliania huxleyi CCMP1516] >gi|551566584|ref|XP_005769584.1| hypothetical protein EMIHUDRAFT_51885, partial [Emiliania huxleyi CCMP1516] >gi|485612138|gb|EOD10528.1| hypothetical protein EMIHUDRAFT_52395, partial [Emiliania huxleyi CCMP1516] >gi|485620587|gb|EOD17155.1| hypothetical protein EMIHUDRAFT_51885, partial [Emiliania huxleyi CCMP1516]) HSP 1 Score: 254.603 bits (649), Expect = 1.029e-71 Identity = 145/378 (38.36%), Postives = 217/378 (57.41%), Query Frame = 0
Query: 397 AWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVI 774
A+ ++ EMR+ PD +Y+ I+ C RS + A+SL+ M G PN+ +YSAAISAC + GR ++A L EM + GL PN+V Y+ +SACE+ G + A LL EM AAG+ +VI+++ IS C++ G+WE+AL LR+M V PD S+NAAI+AC +GG E+ LL M + + +YN + +AG + L +L +M G +P+VISY+ A+SA EK G +E A LL M AG+R N +Y ++ CEK G+W +AL LL M G+ PD Y+ + AC A + G L+L +MQ DGV P ++ Y+ + A + A+ L+ + L R V P+ + +AV+
Sbjct: 16 AFGILDEMRQA-GCEPDVFSYSILINLCARSKDWKTAVSLLREMPGAGVPPNEVSYSAAISACEKAGRWEKALSLLREMPAAGLSPNVVSYSAAISACEKAGRWEEALSLLREMLAAGVQPNVISYSAAISACEKAGQWEKALSLLREMPAAG-VRPDVYSYNAAISACEKGGQWEKALSLLRDMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPAAG--VQPNVISYSAAISACEKAGQWEKALSLLREMPAAGVRPNVISYNAAISACEKGGQWEKALSLLREMPAAGVRPDVISYSAAISACEKAGRCGDALSLFSKMQEDGVQPDVYSYSGAIQACAAAQQPAEALRLFKEAL-RSVEPSCITFNAVL 388 HSP 2 Score: 217.624 bits (553), Expect = 1.172e-58 Identity = 127/355 (35.77%), Postives = 195/355 (54.93%), Query Frame = 0
Query: 278 KLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYT 631
KLI AR G A D+ G P + ++ L ++ R+K W SL +M GV PN+ +Y + + W + L +L A + N V ++ A+ + W+ A +L++EM + P+ ++Y+AAISACE++ ALSL+ M G P+ Y+Y+AAISAC +GG+ ++A L +M GL PN++ YN +SACE+ G D A LL EM AAG+ +VI+++ IS C++ G+WE+AL LR+M V P+ IS+NAAI+AC +GG E+ LL M A V D +Y+ + +AG D L + +M ++G +PDV SY+
Sbjct: 2 KLITAHARVGDRRRAFGILDEMRQAGC-EPDVFSYSILINLCARSKDWKTAVSLLREMPGAGVPPNEVSYSAAISACEKAGRWEKALSLLREMPAAGLSPNVVSYSAAISACEKAGRWEEALSLLREMLAA-GVQPNVISYSAAISACEKAGQWEKALSLLREMPAAGVRPDVYSYNAAISACEKGGQWEKALSLLRDMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPAAGVQPNVISYSAAISACEKAGQWEKALSLLREMPAAG-VRPNVISYNAAISACEKGGQWEKALSLLREMPAAGVRPDVISYSAAISACEKAGRCGDALSLFSKMQEDG--VQPDVYSYS 351 HSP 3 Score: 181.03 bits (458), Expect = 4.682e-46 Identity = 114/342 (33.33%), Postives = 174/342 (50.88%), Query Frame = 0
Query: 456 ISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEG---GGSQVVVGKLEEEG 794
I+A R G A + EM+ G P++ Y+ +++ C R D A LL EM AG+ + ++++ IS C++ G WE+AL LR+M + P+ +S++AAI+AC + G EE LL M A V + +Y+ + +AG + L +L +M G RPDV SY A+SA EK G +E A LL M AG+ N +Y ++ CEK GR +AL LL M G+ P+ Y+ + AC A + L+LL M GV P + Y + A G+ A+ L +M A V P+ + SA I A + G + + K++E+G
Sbjct: 4 ITAHARVGDRRRAFGILDEMRQAGCEPDVFSYSILINLCARSKDWKTAVSLLREMPGAGVPPNEVSYSAAISACEKAGRWEKALSLLREMPAAG-LSPNVVSYSAAISACEKAGRWEEALSLLREMLAAGVQPNVISYSAAISACEKAGQWEKALSLLREMPAAG--VRPDVYSYNAAISACEKGGQWEKALSLLRDMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPAAGVQPNVISYSAAISACEKAGQWEKALSLLREMPAAGVRPNVISYNAAISACEKGGQWEKALSLLREMPAAGVRPDVISYSAAISACEKAGRCGDALSLFSKMQEDG 342 HSP 4 Score: 180.259 bits (456), Expect = 1.032e-45 Identity = 105/303 (34.65%), Postives = 165/303 (54.46%), Query Frame = 0
Query: 490 MLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEE 792
+++A R GD AF +L+EMR AG + DV +++ LI++C +W+ A+ LR+M V P+ +S++AAI+AC + G E+ LL M A + + +Y+ + +AG + + L +L +M G +P+VISY+ A+SA EK G +E A LL M AG+R + Y+Y ++ CEK G+W +AL LL M GL+P+ Y + AC A + L+LL M GV P + Y+ + A G+ A+ L +M A V PN + +A I A +GG + + L E
Sbjct: 3 LITAHARVGDRRRAFGILDEMRQAGCEPDVFSYSILINLCARSKDWKTAVSLLREMPGAG-VPPNEVSYSAAISACEKAGRWEKALSLLREMPAAGLSPNVVSYSAAISACEKAGRWEEALSLLREMLAAG--VQPNVISYSAAISACEKAGQWEKALSLLREMPAAGVRPDVYSYNAAISACEKGGQWEKALSLLRDMPDAGLSPNVISYNAAISACEKAGRCDEALSLLREMPAAGVQPNVISYSAAISACEKAGQWEKALSLLREMPAAGVRPNVISYNAAISACEKGGQWEKALSLLRE 302 HSP 5 Score: 159.458 bits (402), Expect = 1.276e-38 Identity = 104/320 (32.50%), Postives = 165/320 (51.56%), Query Frame = 0
Query: 240 SYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYVFTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFN 559
SY + +AG WE A+ + + + I +AG++E A++ + A G+ P+++ ++ +A QW + SL +M A GVRP+ V +++AA+SA KG W+ A +L+++M + ++P+ ++Y AAISACE++ C ALSL+ M G PN +YSAAISAC + G+ ++A L EM + G+RPN++ YN +SACE+GG + A LL EM AAG+ DVI+++ IS C++ G AL MQ D V+PD S++
Sbjct: 69 SYSAAISACEKAGRWEKALSLLREMPAAGLSPNVVSYSAAISACEKAGRWEEALSLLREMLAAGV-QPNVISYSAAISACEKAGQWEKALSLLREMPAAGVRPD-----VYSYNAAISA-------------------------CEKG----GQWEKALSLLRDMPDA-GLSPNVISYNAAISACEKAGRCDEALSLLREMPAAGVQPNVISYSAAISACEKAGQWEKALSLLREMPAAGVRPNVISYNAAISACEKGGQWEKALSLLREMPAAGVRPDVISYSAAISACEKAGRCGDALSLFSKMQE-DGVQPDVYSYS 351 HSP 6 Score: 101.679 bits (252), Expect = 4.659e-19 Identity = 93/364 (25.55%), Postives = 152/364 (41.76%), Query Frame = 0
Query: 631 TQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEGEGVQGGNREEHGRQGRDGQALIRSERWVDGPVDRAGSPGGQKRKRAPPPLSTGRTRAKTILTRMEREVRRAQVVAGAEKRPGETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGYAARGNAAATNALVREMHRKGVEPNVFTFINLVRACTLAGCPEKALSV 994
T+ ++A +VGD A +L M+ AG + ++Y++++ LC ++ W A+ LL M G+ P+ Y+ + AC A + L+LL M G+ P + Y+ + A G+ A+ L +MLA V PN + SA I A + G + + L E G +R + + A GGQ K LS R L+ V A ++ G ++ S R +P + GV + Y + A + G A +L+ M + P+V +YN + G +L+REM GV P+V ++ + AC AG ALS+
Sbjct: 1 TKLITAHARVGDRRRAFGILDEMRQAGCEPDVFSYSILINLCARSKDWKTAVSLLREMPGAGVPPNEVSYSAAISACEKAGRWEKALSLLREMPAAGLSPNVVSYSAAISACEKAGRWEEALSLLREMLAAGVQPNVISYSAAISACEKAGQWEKALSLLREMPAAG---------------------VRPDVYSYNAAISACEKGGQWEK----ALSLLRDMPDAGLS---PNVISYNAAISACEKAGRCDEALSLLREMPAA--GVQPNVISYSAAISACEKAGQWEKALSLLREMPAAGVRPNVISYNAAISACEKGGQWEKALSLLREMPAAGVRPDVISYSAAISACEKAGRCGDALSL 334
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|299471045|emb|CBN78905.1| (conserved unknown protein [Ectocarpus siliculosus]) HSP 1 Score: 261.922 bits (668), Expect = 1.508e-70 Identity = 162/539 (30.06%), Postives = 267/539 (49.54%), Query Frame = 0
Query: 240 SYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHAL 777
SY + +K GR G WE +G+ + + +I + G+++ A++ + A GL P +I +N GR+ +W + +M + GV P+ +Y + W + +LD A N + F A+ W+ A L++E+ +TPD ++ +AI+AC +S AL L+ M P+ +Y++AI ACG+GG+ + A L +M ++GL PN++ N + AC G +LL +M A G+ +VIT+N+ I C + G+WE+AL L M+ L + PD+I++N+AIAAC++ G +E LL M Q + D +Y +D Y + G + + +L QM +G P+VI+Y + A + G++E A LL +K + + +Y + ++ C K GRW +AL LL M GLTPD YT ++AC + + L LL M GV PT Y++ + A G+ AV L +M R + + S ++ AL
Sbjct: 217 SYNSAIKACGRGGQWERVIGLLREMPSVGLTPDAITYNSVITGCGKEGQWKEALSVLTEMSAKGLTPETI-SYNMAIRACGRSGRWKEAVEVLRQMESQGVTPDVISYDAAIKACGGGGQWETSVDLLDEMAGRGVAPNTIHFNSAIVACGKGGQWEKAVELLREVTAL-GLTPDATSFNSAIAACTKSGRWKEALELLKEMPAKRLKPDAISYNSAIEACGKGGQWEMALELRRQMPTKGLTPNVISSNIAIRACGERGRWQEGLELLRQMPAQGLTPNVITYNSAIKTCGKGGQWEKALDLLAKMKELA-MTPDSITYNSAIAACSKRGRWKEAVALLREMPGQGLTPDVISYTAAIDAYGKNGQWERAVELLRQMPTKG--LTPNVITYNSVIKACGRGGEWEKALDLLKELKETAVAPDLMSYNLAISACGKRGRWEEALDLLREMPAEGLTPDVISYTSAIRACNAEGEWEKALGLLNLMGAHGVSPTATSYSLAIEACGKGGRREEAVCLVREMAQRGLSHRNISNSLIVEAL 750 HSP 2 Score: 258.07 bits (658), Expect = 3.441e-69 Identity = 165/568 (29.05%), Postives = 273/568 (48.06%), Query Frame = 0
Query: 231 EGSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEG 797
EG P +V Y + ++ G WE AV + + R + I+ R G++E + + ++GL P +I +N + G+ QW S+ +M A G+ P +Y + + S W E + +L + + + A+K W+ + L+ EM R + P+T+ + +AI AC + A+ L+ + G P+ ++++AI+AC + GR EA L EM ++ L+P+ + YN+ + AC +GG + A +L +M G+ +VI+ N I C ERG W+ L LR M + P+ I++N+AI C +GG E+ LL +MK + D+ TYN+ + ++ G + + + +L +M +G PDVISYT A+ A K G +E A LL M G+ N TY V+ C + G W +AL LL ++ + PD Y + + AC + L LL M +G+ P + YT + A +G+ A+GL + M A V P S I A +GG + V + E + G
Sbjct: 174 EGVSP-NVFCYNSAIEACGSGDQWEIAVSLLREMADREVVPDEISYNSAIKACGRGGQWERVIGLLREMPSVGLTPDAI-TYNSVITGCGKEGQWKEALSVLTEMSAKGLTPETISYNMAIRACGRSGRWKEAVEVLRQMESQGVTPDVISYDAAIKACGGGGQWETSVDLLDEMAGR-GVAPNTIHFNSAIVACGKGGQWEKAVELLREVTALGLTPDATSFNSAIAACTKSGRWKEALELLKEMPAKRLKPDAISYNSAIEACGKGGQWEMALELRRQMPTKGLTPNVISSNIAIRACGERGRWQEGLELLRQMPA-QGLTPNVITYNSAIKTCGKGGQWEKALDLLAKMKELAMTPDSITYNSAIAACSKRGRWKEAVALLREMPGQG--LTPDVISYTAAIDAYGKNGQWERAVELLRQMPTKGLTPNVITYNSVIKACGRGGEWEKALDLLKELKETAVAPDLMSYNLAISACGKRGRWEEALDLLREMPAEGLTPDVISYTSAIRACNAEGEWEKALGLLNLMGAHGVSPTATSYSLAIEACGKGGRREEAVCLVREMAQRG 735 HSP 3 Score: 220.32 bits (560), Expect = 2.645e-56 Identity = 136/468 (29.06%), Postives = 220/468 (47.01%), Query Frame = 0
Query: 348 QVLAHSAAVSAPWSEVLRILDAATLASCASNYVFTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQT-----------------------------LDR-----VEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGG 781
Q AA + W E L ILD + +T A+ + W+ A L++E+ E+ ++P+ Y +AI AC +A+SL+ M P++ +Y++AI ACGRGG+ + L EM S GL P+ + YN++++ C + G A +L EM A G+ + I++N I C G W+ A+ LR M++ LD V P+TI FN+AI AC +GG E+ LL + A + D ++N+ + T++G + + L +L +M + R +PD ISY A+ A K G +E A L M G+ N + + + C + GRW + L LL M +GLTP+ Y +K C + L LL +M+ + P Y + A +G+ + AV L +M + + P+ + +A I A + G
Sbjct: 114 QAALREAATTGKWREALGILDEMKEGGVVCAHSYTTAITTCGRQGQWEKALELLREIPEQEGVSPNVFCYNSAIEACGSGDQWEIAVSLLREMADREVVPDEISYNSAIKACGRGGQWERVIGLLREMPSVGLTPDAITYNSVITGCGKEGQWKEALSVLTEMSAKGLTPETISYNMAIRACGRSGRWKEAVEVLRQMESQGVTPDVISYDAAIKACGGGGQWETSVDLLDEMAGRGVAPNTIHFNSAIVACGKGGQWEKAVELLREVTALGLTPDATSFNSAIAACTKSGRWKEALELLKEMPAK--RLKPDAISYNSAIEACGKGGQWEMALELRRQMPTKGLTPNVISSNIAIRACGERGRWQEGLELLRQMPAQGLTPNVITYNSAIKTCGKGGQWEKALDLLAKMKELAMTPDSITYNSAIAACSKRGRWKEAVALLREMPGQGLTPDVISYTAAIDAYGKNG 579 HSP 4 Score: 193.741 bits (491), Expect = 1.677e-47 Identity = 166/614 (27.04%), Postives = 272/614 (44.30%), Query Frame = 0
Query: 450 YTYSAAISACGRGGRLDEAKRLWGEM-KSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEGEGVQGGNREEHGRQGRDGQALIRSERWVDGPVDRAGSPGGQKRKRAPPPLSTGRTRAKTILTRMER------EVRRAQVVAGAEKRPGETEDPRSGERVVPGSGQGVCLDRREYHVLLRAICRKGHLAFAHEILQHMVRVHLPPDVTAYNYLVLGYAARGNAAATNALVREMHRKGVEPNVFTFINLVRACTLAGCPEKALSVVD--PA--FSWDF--YSAAITIYHQSGCFAEADALYQQAVLHGKVPVDVTLTPADHALDLHG 1050
++Y+ AI+ CGR G+ ++A L E+ + G+ PN+ YN+ + AC G + A LL EM + D I++N+ I C G+WER +G LR+M ++ + PD I++N+ I C + G +E +L M A+ + +T +YN + R+G + + + VL QM +G PDVISY A+ A G +E + LL M G+ N + + C K G+W +A+ LL + GLTPD+T + + AC + + L LL+ M + P Y + A G+ A+ L M + + PN + S + + E G+ EG+ E RQ Q L + + + G GGQ K A +L +M+ + +A KR G ++ + R +PG QG+ D Y + A + G A E+L+ M L P+V YN ++ G L++E+ V P++ ++ + AC G E+AL ++ PA + D Y++AI + G + +A L HG P + + A A G
Sbjct: 145 HSYTTAITTCGRQGQWEKALELLREIPEQEGVSPNVFCYNSAIEACGSGDQWEIAVSLLREMADREVVPDEISYNSAIKACGRGGQWERVIGLLREMPSVG-LTPDAITYNSVITGCGKEGQWKEALSVLTEMSAKGLTPETISYNMAIRACGRSGRWKEAVEVLRQMESQG--VTPDVISYDAAIKACGGGGQWETSVDLLDEMAGRGVAPNTIHFNSAIVACGKGGQWEKAVELLREVTALGLTPDATSFNSAIAACTKSGRWKEALELLKEMPAKRLKPDAISYNSAIEACGKGGQWEMALELRRQMPTKGLTPNVI-------------SSNIAIRACGERGRWQEGL------ELLRQ-MPAQGLTPNVITYNSAIKTCGK-GGQWEK------------ALDLLAKMKELAMTPDSITYNSAIAACSKR-GRWKEAVALLREMPG--QGLTPDVISYTAAIDAYGKNGQWERAVELLRQMPTKGLTPNVITYNSVIKACGRGGEWEKALDLLKELKETAVAPDLMSYNLAISACGKRGRWEEALDLLREMPAEGLTPDVISYTSAIRACNAEGEWEKALGLLNLMGAHGVSPTATSYSLAIEACGKGG 719
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|551543901|ref|XP_005760066.1| (hypothetical protein EMIHUDRAFT_51716, partial [Emiliania huxleyi CCMP1516] >gi|485608338|gb|EOD07637.1| hypothetical protein EMIHUDRAFT_51716, partial [Emiliania huxleyi CCMP1516]) HSP 1 Score: 248.44 bits (633), Expect = 4.868e-69 Identity = 139/370 (37.57%), Postives = 204/370 (55.14%), Query Frame = 0
Query: 397 AWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPN 766
A+ L+ EMR+ PD ++Y+ I+ C S + A+SL+ M G PN +YSAAISAC + GR +A L EM G++P++ Y+ +SACE+ G D A L +EM AAG+ DVI++N IS C++ G+WE+AL LR+M + PD IS++AAI+AC + E L M A V + ++N + +AG + L +L +M G PDVISY+ A+SA+EK G +E A LL M AG+ N+++Y+ ++ CEK GR +AL LL M GL P+ Y+ + AC A + LAL + M GV PT+ Y+ + A G+ A+ L+ M V PN
Sbjct: 25 AFGLLDEMRQA-GCEPDVISYSILINMCAGSKDWKTAVSLLREMPDAGVPPNVVSYSAAISACAKAGRWQKAVSLLREMPGAGVQPDVYSYSAAISACEKAGRCDEALSLFDEMPAAGVQPDVISYNATISACEKGGQWEKALSLLREMPGAG-LSPDVISYSAAISACEKASRCNEALSLFDEMLAAGVRPNVISFNATISACEKAGRCDEALLLLREMPDAG--LSPDVISYSAAISASEKAGQWEKAVSLLREMPAAGVPPNEFSYSAAISACEKAGRCDEALSLLREMPAAGLRPNVISYSAAISACEKASRCDEALALFDEMLAAGVQPTVISYSAAISACEKAGRCGDALSLFSKMQEDGVQPN 390 HSP 2 Score: 241.891 bits (616), Expect = 1.135e-66 Identity = 143/413 (34.62%), Postives = 224/413 (54.24%), Query Frame = 0
Query: 366 ILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHAL 777
+LD A C + + +++ + ++WK A +L++EM + + P+ V+Y+AAISAC ++ A+SL+ M G P+ Y+YSAAISAC + GR DEA L+ EM + G++P+++ YN +SACE+GG + A LL EM AG+ DVI+++ IS C++ AL +M V P+ ISFNA I+AC + G +E LL M + D +Y+ + +AG + + +L +M G P+ SY+ A+SA EK G + A LL M AG+R N +Y+ ++ CEK R +AL L M G+ P Y+ + AC A + G L+L +MQ DGV P ++ Y+ + A + A+ L+ L R V P+ V +AV+ A+
Sbjct: 28 LLDEMRQAGCEPDVISYSILINMCAGSKDWKTAVSLLREMPDA-GVPPNVVSYSAAISACAKAGRWQKAVSLLREMPGAGVQPDVYSYSAAISACEKAGRCDEALSLFDEMPAAGVQPDVISYNATISACEKGGQWEKALSLLREMPGAGLSPDVISYSAAISACEKASRCNEALSLFDEMLAAG-VRPNVISFNATISACEKAGRCDEALLLLREMPDAGLSPDVISYSAAISASEKAGQWEKAVSLLREMPAAG--VPPNEFSYSAAISACEKAGRCDEALSLLREMPAAGLRPNVISYSAAISACEKASRCDEALALFDEMLAAGVQPTVISYSAAISACEKAGRCGDALSLFSKMQEDGVQPNVYSYSAAIQACAAAQQPDEALRLFKAAL-RSVEPSRVTFNAVLDAV 435 HSP 3 Score: 207.223 bits (526), Expect = 1.653e-54 Identity = 138/434 (31.80%), Postives = 219/434 (50.46%), Query Frame = 0
Query: 274 KEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASN-YVFTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKA 706
KE+ +LI AR G A D+ G P ++ ++ L ++ +K W SL +M GV PN +Y + A + W + + +L A + Y ++ A+ + A +L EM + PD ++Y A ISACE+ ALSL+ M G P+ +YSAAISAC + R +EA L+ EM + G+RPN++ +N +SACE+ G D A LL EM AG+ DVI+++ IS ++ G+WE+A+ LR+M V P+ S++AAI+AC + G +E LL M A + + +Y+ + +A + L + D+M G +P VISY+ A+SA EK G A L + M+ G++ N Y+Y+ + C + +AL L + R + P + V+ A
Sbjct: 7 KEWTQLISAHARVGDCRRAFGLLDEMRQAGC-EPDVISYSILINMCAGSKDWKTAVSLLREMPDAGVPPNVVSYSAAISACAKAGRWQKAVSLLREMPGAGVQPDVYSYSAAISACEKAGRCDEALSLFDEMPAA-GVQPDVISYNATISACEKGGQWEKALSLLREMPGAGLSPDVISYSAAISACEKASRCNEALSLFDEMLAAGVRPNVISFNATISACEKAGRCDEALLLLREMPDAGLSPDVISYSAAISASEKAGQWEKAVSLLREMPAAG-VPPNEFSYSAAISACEKAGRCDEALSLLREMPAAGLRPNVISYSAAISACEKASRCDEALALFDEMLAAG--VQPTVISYSAAISACEKAGRCGDALSLFSKMQEDGVQPNVYSYSAAIQACAAAQQPDEALRLFKAA-LRSVEPSRVTFNAVLDA 434 HSP 4 Score: 195.667 bits (496), Expect = 1.644e-50 Identity = 123/385 (31.95%), Postives = 189/385 (49.09%), Query Frame = 0
Query: 413 DTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEG---GGSQVVVGKLEEEG 794
DT +T ISA R +C A L+ M G P+ +YS I+ C A L EM G+ PN+V Y+ +SAC + G A LL EM AG+ DV +++ IS C++ G + AL +M V+PD IS+NA I+AC +GG E+ LL M + D +Y+ + +A + L + D+M G RP+VIS+ +SA EK G + A LL M AG+ + +Y+ ++ EK G+W +A+ LL M G+ P+ Y+ + AC A + L+LL M G+ P + Y+ + A + A+ L+ +MLA V P + SA I A + G + + K++E+G
Sbjct: 5 DTKEWTQLISAHARVGDCRRAFGLLDEMRQAGCEPDVISYSILINMCAGSKDWKTAVSLLREMPDAGVPPNVVSYSAAISACAKAGRWQKAVSLLREMPGAGVQPDVYSYSAAISACEKAGRCDEALSLFDEMPAAG-VQPDVISYNATISACEKGGQWEKALSLLREMPGAGLSPDVISYSAAISACEKASRCNEALSLFDEMLAAG--VRPNVISFNATISACEKAGRCDEALLLLREMPDAGLSPDVISYSAAISASEKAGQWEKAVSLLREMPAAGVPPNEFSYSAAISACEKAGRCDEALSLLREMPAAGLRPNVISYSAAISACEKASRCDEALALFDEMLAAGVQPTVISYSAAISACEKAGRCGDALSLFSKMQEDG 386 HSP 5 Score: 169.859 bits (429), Expect = 1.054e-41 Identity = 121/375 (32.27%), Postives = 193/375 (51.47%), Query Frame = 0
Query: 235 PLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGK----EFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAP-----WSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDG 599
P +V SY + +AG W+ AV + R G G + + I +AG+ + A++ +D+ A G+ P ++ +N + QW + SL +M G+ P+ V+++SAA+SA +E L + D A N + F + + A L++EM + ++PD ++Y+AAISA E++ A+SL+ M G PN+++YSAAISAC + GR DEA L EM + GLRPN++ Y+ +SACE+ D A L +EM AAG+ VI+++ IS C++ G AL MQ D V+P+ S++AAI ACA +E RL + + V+ T+N ++D
Sbjct: 73 PPNVVSYSAAISACAKAGRWQKAVSLL----REMPGAGVQPDVYSYSAAISACEKAGRCDEALSLFDEMPAAGVQ-PDVISYNATISACEKGGQWEKALSLLREMPGAGLSPD-----VISYSAAISACEKASRCNEALSLFDEMLAAGVRPNVISFNATISACEKAGRCDEALLLLREMPDA-GLSPDVISYSAAISASEKAGQWEKAVSLLREMPAAGVPPNEFSYSAAISACEKAGRCDEALSLLREMPAAGLRPNVISYSAAISACEKASRCDEALALFDEMLAAGVQPTVISYSAAISACEKAGRCGDALSLFSKMQE-DGVQPNVYSYSAAIQACAAAQQPDEALRLF-KAALRSVEPSRVTFNAVLDA 434
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|551540954|ref|XP_005758806.1| (hypothetical protein EMIHUDRAFT_53301, partial [Emiliania huxleyi CCMP1516] >gi|485606643|gb|EOD06377.1| hypothetical protein EMIHUDRAFT_53301, partial [Emiliania huxleyi CCMP1516]) HSP 1 Score: 243.817 bits (621), Expect = 9.169e-68 Identity = 141/394 (35.79%), Postives = 208/394 (52.79%), Query Frame = 0
Query: 366 ILDAATLASCASNYVFTMALKGLKVR-RNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDM 758
ILD A C N L L R ++WK A +L++EM + P+ ++Y AAISACE++ C ALSL+ M G P+ +Y+AAISAC + GR DEA L EM G++P+++ Y+ +SACE+ G D A LL EM AG+ + ++N IS C++ G WE A+ LR+M + P+ +S++AAI+AC + G +E LL M + + +YN +AG + L +L +M G +PDVISY A+SA EK G A LL M AG++ + +Y ++ CEK RW +AL LL M G+ PD Y+ + AC A + L+LL M V P + Y + + G+ A+ L +M
Sbjct: 1 ILDEMRQAGCEPNVFSYSILINLCARSKDWKTAVSLLREM-PNAGVQPNVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAG-LRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAATSACEKAGRCDEALSLLREM--PGAGVQPDVISYNAAISACEKAGRCNEALSLLREMPSAGVQPDVISYNAAISACEKASRWEKALSLLREMPDVGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREM 390 HSP 2 Score: 234.572 bits (597), Expect = 1.726e-64 Identity = 140/389 (35.99%), Postives = 210/389 (53.98%), Query Frame = 0
Query: 307 PSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLT 694
P++ ++ L ++ R+K W SL +M GV+PN +Y + + E L +L + + + A+ + A +L++EM + + PD ++Y+AAISACE++ C ALSL+ M G PN+Y+Y+AAISAC + GR +EA L EM + GLRPN+V Y+ +SACE+ G D A LL EM AG+ +VI++N S C++ G + AL LR+M V+PD IS+NAAI+AC + G E LL M + V D +YN + +A + L +L +M G +PDVISY+ A+SA EK + A LL M A ++ + +Y ++ CEK GR +AL LL M GL+
Sbjct: 12 PNVFSYSILINLCARSKDWKTAVSLLREMPNAGVQPNVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYNAAISACEKAGRCDEALSLLREMPD-VGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAGLSPNVISYNAATSACEKAGRCDEALSLLREMPGAG-VQPDVISYNAAISACEKAGRCNEALSLLREMPSAGVQPDVISYNAAISACEKASRWEKALSLLREMPDVG--VQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGLS 396 HSP 3 Score: 229.565 bits (584), Expect = 9.299e-63 Identity = 139/399 (34.84%), Postives = 209/399 (52.38%), Query Frame = 0
Query: 331 LYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGV 728
+ ++M G PN +Y +L + A S W + +L A N + + A+ + A +L++EM + + PD ++Y AAISACE++ C ALSL+ M G P+ +YSAAISAC + GR DEA L EM G++PN YN +SACE+ G + A LL EM AAG+ +V++++ IS C++ G + AL LR+M + P+ IS+NAA +AC + G +E LL M V D +YN + +AG + L +L +M G +PDVISY A+SA EK +E A LL M G++ + +Y+ ++ CEK R +AL LL M + PD Y + +C A + L+LL M + G+
Sbjct: 1 ILDEMRQAGCEPNVFSYSILINLCARSKDWKTAVSLLREMPNAGVQPNVISYNAAISACEKAGRCDEALSLLREMPD-VGVQPDVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAGVQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAG-LSPNVISYNAATSACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRCNEALSLLREMPSAG--VQPDVISYNAAISACEKASRWEKALSLLREMPDVGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLREMPDAGL 395 HSP 4 Score: 226.868 bits (577), Expect = 7.976e-62 Identity = 137/393 (34.86%), Postives = 205/393 (52.16%), Query Frame = 0
Query: 400 LIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEE 792
++ EMR+ P+ +Y+ I+ C RS + A+SL+ M G PN +Y+AAISAC + GR DEA L EM G++P+++ YN +SACE+ G D A LL EM G+ DVI+++ IS C++ G + AL LR+M V+P+ S+NAAI+AC + G EE LL M A + + +Y+ + +AG + L +L +M G P+VISY A SA EK G + A LL M AG++ + +Y ++ CEK GR +AL LL M G+ PD Y + AC A + L+LL M + GV P + Y+ + A + A+ L +M RV P+ + +A I + + G + L E
Sbjct: 1 ILDEMRQA-GCEPNVFSYSILINLCARSKDWKTAVSLLREMPNAGVQPNVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYNAAISACEKAGRCDEALSLLREMPDVGVQPDVISYSAAISACEKAGRCDEALSLLREMPGAG-VQPNEYSYNAAISACEKAGRWEEAVSLLREMPAAGLRPNVVSYSAAISACEKAGRCDEALSLLREMPDAG--LSPNVISYNAATSACEKAGRCDEALSLLREMPGAGVQPDVISYNAAISACEKAGRCNEALSLLREMPSAGVQPDVISYNAAISACEKASRWEKALSLLREMPDVGVQPDVISYSAAISACEKASRCDEALSLLREMPGARVQPDVISYNAAISSCEKAGRCNEALSLLRE 389
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|299471515|emb|CBN80001.1| (conserved unknown protein [Ectocarpus siliculosus]) HSP 1 Score: 249.595 bits (636), Expect = 8.605e-67 Identity = 154/550 (28.00%), Postives = 265/550 (48.18%), Query Frame = 0
Query: 231 EGSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGK--EFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHAL 777
EG P V +Y + + + G W+ A+ + + +G+ + I + G+++ AM ++ A G+ PP+++ +N + ++W L E++ G P+ TY + + A W E + +L ++ + ++ + R WK A L+++MR + ++P+ ++Y +AI AC + AL L+ M G P +Y++A +AC GR EA L EM ++G+ PN + YN+ L AC +GG + A LL M G D D+I++N+ I C +WE A+ LR+M T + P+ I++N+AI AC R +E L M + + + TYN+++D + + + +L M G PDVISY A+ A K +E A +LL M G + + +Y + C K+GRW +AL LL M GLTP++ Y + AC ++ + +L+ MQ G P + Y+ + A +G+ + AV L D+ + + P+ VI A
Sbjct: 139 EGVTP-DVVTYNSAIAACSKGGRWKEAMDLLTQMVAPTEGITPDVVTYNSAIAACSNGGRWKEAMDLLEQMVAQGV-PPNLITYNSAIGACAKGRRWEEAMDLLEEVIEQGFPPSTRTYNPVIDACAKGGRWLEAMDLLGQMLTNDIPADVISYSSVIAACGRGRRWKEAMDLLEQMRTQ-GVSPNVISYNSAIDACAKGDRWKEALDLLREMTTVGLVPTVISYNSATAACAVNGRWVEALELLKEMPAQGIAPNTISYNSALDACAKGGQWEKAVKLLRGMSTVGSDPDIISFNSAIDACGRGQQWETAVELLREMPTAG-LTPNVITYNSAIEACGRSARWQEAMGLFREMPTRGLSPNVVTYNSMIDACAKGEQWELAVQLLTGMPARG--VAPDVISYNSAIEACGKGEQWELALQLLKGMPTRGPKPDIISYNSAVTACAKSGRWREALGLLKDMATVGLTPNTVSYGAAIHACGKGEQWDVAVRILKEMQTHGATPNLITYSAAIDACAKQGRWKEAVDLLTDLRRQGLTPDAQTYLTVISAF 682 HSP 2 Score: 232.261 bits (591), Expect = 8.266e-61 Identity = 162/570 (28.42%), Postives = 260/570 (45.61%), Query Frame = 0
Query: 232 GSRPLS-VHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFA--MGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEG 797
G +P S V L++ GR DW A+ + + + + I + ++ A+ + G+ P ++ +N + +W L +M A G+ P+ TY + + W E + +L+ N + + A+ R W+ A L++E+ E+ P T TY I AC + A+ L+ M + + +YS+ I+ACGRG R EA L +M+++G+ PN++ YN+ + AC +G A DLL EM G+ VI++N+ + C G W AL L++M + P+TIS+N+A+ ACA+GG E+ +LL M D D ++N+ +D R + + +L +M G P+VI+Y A+ A + ++ A L M G+ N TY ++ C K +W A+ LL M RG+ PD Y ++AC ++ L LL+ M G P I Y V A G+ R A+GL DM + PN V A IHA +G V V L+E G
Sbjct: 70 GGKPKSLVKLKSILLEAKGR--DWRTALAALDEFKISGGSLDTTSYNIAIAACSNGRQWATAVRLLREMPTEGV-TPDVVTYNSAIAACSKGGRWKEAMDLLTQMVAPTEGITPDVVTYNSAIAACSNGGRWKEAMDLLEQMVAQGVPPNLITYNSAIGACAKGRRWEEAMDLLEEVIEQ-GFPPSTRTYNPVIDACAKGGRWLEAMDLLGQMLTNDIPADVISYSSVIAACGRGRRWKEAMDLLEQMRTQGVSPNVISYNSAIDACAKGDRWKEALDLLREMTTVGLVPTVISYNSATAACAVNGRWVEALELLKEMPA-QGIAPNTISYNSALDACAKGGQWEKAVKLLRGMSTVGSDPDIISFNSAIDACGRGQQWETAVELLREMPTAG--LTPNVITYNSAIEACGRSARWQEAMGLFREMPTRGLSPNVVTYNSMIDACAKGEQWELAVQLLTGMPARGVAPDVISYNSAIEACGKGEQWELALQLLKGMPTRGPKPDIISYNSAVTACAKSGRWREALGLLKDMATVGLTPNTVSYGAAIHACGKGEQWDVAVRILKEMQTHG 632 HSP 3 Score: 221.476 bits (563), Expect = 3.536e-57 Identity = 164/577 (28.42%), Postives = 255/577 (44.19%), Query Frame = 0
Query: 227 FSLLEGSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLG-PPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATL-----ASCASNYVFTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEG 797
F + GS L SY + W AV + + + I ++ G+++ AM + A G P ++ +N +W L E+M A GV PN TY + A W E + +L+ ++ N V KG + W A L+ +M I D ++Y++ I+AC R A+ L+ M G PN +Y++AI AC +G R EA L EM + GL P ++ YN+ +AC G A +LL+EM A GI + I++N+ + C + G+WE+A+ LR M T+ +PD ISFN+AI AC RG E LL M + + TYN+ ++ R+ + + + + +M G P+V++Y + A K +E A +LL M G+ + +Y + C K +W AL LL M RG PD Y V ACA + + L LL+ M G+ P Y + A + AV + +M PN + SA I A + G + V L + ++G
Sbjct: 101 FKISGGS--LDTTSYNIAIAACSNGRQWATAVRLLREMPTEGVTPDVVTYNSAIAACSKGGRWKEAMDLLTQMVAPTEGITPDVVTYNSAIAACSNGGRWKEAMDLLEQMVAQGVPPNLITYNSAIGACAKGRRWEEAMDLLEEVIEQGFPPSTRTYNPVIDACAKGGR----WLEAMDLLGQMLTN-DIPADVISYSSVIAACGRGRRWKEAMDLLEQMRTQGVSPNVISYNSAIDACAKGDRWKEALDLLREMTTVGLVPTVISYNSATAACAVNGRWVEALELLKEMPAQGIAPNTISYNSALDACAKGGQWEKAVKLLRGMSTVGS-DPDIISFNSAIDACGRGQQWETAVELLREMPTAGLTPNVITYNSAIEACGRSARWQEAMGLFREMPTRG--LSPNVVTYNSMIDACAKGEQWELAVQLLTGMPARGVAPDVISYNSAIEACGKGEQWELALQLLKGMPTRGPKPDIISYNSAVTACAKSGRWREALGLLKDMATVGLTPNTVSYGAAIHACGKGEQWDVAVRILKEMQTHGATPNLITYSAAIDACAKQGRWKEAVDLLTDLRRQG 667 HSP 4 Score: 198.364 bits (503), Expect = 1.819e-49 Identity = 145/536 (27.05%), Postives = 235/536 (43.84%), Query Frame = 0
Query: 179 HSFRSSTRNATRWIEAA----HINGTALPPQDYLL---LKDLVRRREAMQARAFCFSLLEGSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKA 706
+S ++ N RW EA + +PP + + R +A ++E P S +Y ++ + G W A+ + + + +I R +++ AM ++ G+ P +++ +N D + +W L +M +G+ P +Y + AV+ W E L +L A N + + AL W+ A L++ M PD +++ +AI AC R A+ L+ M G PN TY++AI ACGR R EA L+ EM +RGL PN+V YN+M+ AC +G + A LL M A G+ DVI++N+ I C + +WE AL L+ M T +PD IS+N+A+ ACA+ G E LL M + +T +Y + + + + +L +M G A P++I+Y+ A+ A K G RW +A+ LL +RR+GLTPD+ Y V+ A
Sbjct: 186 NSAIAACSNGGRWKEAMDLLEQMVAQGVPPNLITYNSAIGACAKGRRWEEAMDLLEEVIEQGFPPSTRTYNPVIDACAKGGRWLEAMDLLGQMLTNDIPADVISYSSVIAACGRGRRWKEAMDLLEQMRTQGVSP-NVISYNSAIDACAKGDRWKEALDLLREMTTVGLVPTVISYNSATAACAVNGRWVEALELLKEMPAQGIAPNTISYNSALDACAKGGQWEKAVKLLRGMSTV-GSDPDIISFNSAIDACGRGQQWETAVELLREMPTAGLTPNVITYNSAIEACGRSARWQEAMGLFREMPTRGLSPNVVTYNSMIDACAKGEQWELAVQLLTGMPARGVAPDVISYNSAIEACGKGEQWELALQLLKGMPTRGP-KPDIISYNSAVTACAKSGRWREALGLLKDMATVGLTPNTVSYGAAIHACGKGEQWDVAVRILKEMQTHG--ATPNLITYSAAIDACAKQG-----------------RWK------------------EAVDLLTDLRRQGLTPDAQTYLTVISA 681 HSP 5 Score: 111.309 bits (277), Expect = 3.183e-21 Identity = 75/269 (27.88%), Postives = 125/269 (46.47%), Query Frame = 0
Query: 232 GSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGD 499
GS P + S+ + + GR WE AV + + + I R+ +++ AM + + GL P +++ +N + D + +QW L M A GV P+ +Y + W L++L + + + A+ W+ A L+++M +TP+TV+Y AAI AC + VA+ ++ M+ GA PN TYSAAI AC + GR EA L +++ +GL P+ Y T++SA +R D
Sbjct: 422 GSDP-DIISFNSAIDACGRGQQWETAVELLREMPTAGLTPNVITYNSAIEACGRSARWQEAMGLFREMPTRGLSP-NVVTYNSMIDACAKGEQWELAVQLLTGMPARGVAPDVISYNSAIEACGKGEQWELALQLLKGMPTRGPKPDIISYNSAVTACAKSGRWREALGLLKDMATV-GLTPNTVSYGAAIHACGKGEQWDVAVRILKEMQTHGATPNLITYSAAIDACAKQGRWKEAVDLLTDLRRQGLTPDAQTYLTVISAFQRCSD 687
BLAST of EWM21396.1 vs. NCBI_GenBank
Match: gi|299471535|emb|CBN80021.1| (conserved unknown protein [Ectocarpus siliculosus]) HSP 1 Score: 240.35 bits (612), Expect = 2.698e-64 Identity = 161/563 (28.60%), Postives = 264/563 (46.89%), Query Frame = 0
Query: 239 HSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEEEGKEGEGV 800
SY + GR G WE AV + + + +I A+ G+++ A+ + + G+ P + + + R +W L +M A V PN Y +S A + W + ++ A + + ++ A+ W+ A L ++MR + PD +TY +AI+AC A+SL+ + G PN +YS+ I+AC + G+ A L EMK+ L PN++ YN + AC +GG A DLL EM G+ DV++++++I C + W+ A+ LR+M T+ + P+ IS+N+AI ACA+GG +E + LL M V + YN+ + + + + L +L +M G V SY+ A+ A K + A LL M G+ N+ Y + C + +W +A+ LL M GL+PD Y+ + ACA + LA+L+ M G+ P + Y + A G+ + AV L +M A + PN + S+ I A + GG + + LEE G GV
Sbjct: 34 KSYCMAITACGRGGQWEQAVRLLREMPTEGAAPDLSAYSAVIDACAKGGQWKMAVFFLMEMPTKGIAPDA-RSYGAAINACARGGRWKIALDLLREMLARDVTPNVIIYNSAINSCAKAGQWEIAVSLVKEMATVGLAPDVISYSSAISACGRGGRWEEALELFEDMRTS-GVAPDVITYGSAIAACANGGRWKEAVSLLRKIPTVGLTPNVISYSSVITACAKEGQWKIALDLLREMKAMRLAPNIITYNAAIDACAKGGRWKEAIDLLREMPTVGLPPDVVSYSSVIDACSKGDRWKEAIDILREMPTVG-LSPNAISYNSAIDACAKGGQWKEAKGLLREMPTAGVTQRVIGYNSAIAACAKGEQWKEALALLREMPTVG--LHTTVFSYSSAIDACGKGNLWIKAKELLREMATVGLAPNEVCYNSAIDACGRGDQWEEAVDLLREMPTVGLSPDVFSYSSAIAACAKGDQWKEALAVLKEMSAAGLAPDLICYNSAIDACSKGGRWKMAVALLGEMRAAGLTPNIISYSSAIDACVRGGQWKEGIALLEE--MRGSGV 589 HSP 2 Score: 230.72 bits (587), Expect = 5.993e-61 Identity = 154/554 (27.80%), Postives = 273/554 (49.28%), Query Frame = 0
Query: 231 EGSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAP-----WSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALL 778
EG+ P + +Y ++ + G W+ AV + + + + I AR G+++ A+ + A + P+++ +N + +A QW SL ++M +G+ P+ V+++S+A+SA W E L + + + A + + + A+ WK A +L++++ +TP+ ++Y++ I+AC + +AL L+ M+ PN TY+AAI AC +GGR EA L EM + GL P++V Y++++ AC +G A D+L EM G+ + I++N+ I C + G+W+ A G LR+M T V I +N+AIAACA+G +E LL M + +Y++ +D + + +L +M G P+ + Y A+ A + +E A LL M G+ + ++Y+ +A C K +W +AL +L M GL PD CY + AC+ + +ALL M+ G+ P I Y+ + A + G+ + + L +M V P+ + HAL+
Sbjct: 62 EGAAP-DLSAYSAVIDACAKGGQWKMAVFFLMEMPTKGIAPDARSYGAAINACARGGRWKIALDLLREMLARDV-TPNVIIYNSAINSCAKAGQWEIAVSLVKEMATVGLAPD-----VISYSSAISACGRGGRWEEALELFEDMRTSGVAPDVITYGSAIAACANGGRWKEAVSLLRKIPTV-GLTPNVISYSSVITACAKEGQWKIALDLLREMKAMRLAPNIITYNAAIDACAKGGRWKEAIDLLREMPTVGLPPDVVSYSSVIDACSKGDRWKEAIDILREMPTVGLSPNAISYNSAIDACAKGGQWKEAKGLLREMPTAG-VTQRVIGYNSAIAACAKGEQWKEALALLREMPTVGLHTTVFSYSSAIDACGKGNLWIKAKELLREMATVG--LAPNEVCYNSAIDACGRGDQWEEAVDLLREMPTVGLSPDVFSYSSAIAACAKGDQWKEALAVLKEMSAAGLAPDLICYNSAIDACSKGGRWKMAVALLGEMRAAGLTPNIISYSSAIDACVRGGQWKEGIALLEEMRGSGVVPDVI----TYHALM 600 HSP 3 Score: 228.794 bits (582), Expect = 3.005e-60 Identity = 152/555 (27.39%), Postives = 266/555 (47.93%), Query Frame = 0
Query: 277 MKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILD---FNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWS-EVLRILDAATLASCASNYVFTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGI------------------RWNQ-----------------YTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACASAQKLGHCLALLERMQNDGVVPTIHIYTILVGAALMKGKARTAVGLYHDMLARRVHPNGVFVSAVIHALLEGGGSQVVVGKLEE 792
MK + +R+G + A+ D + L P ++D + GR QW + L +M G P+ S Y + + A W V +++ T + A+ WK A L++EM R +TP+ + Y +AI++C ++ +A+SLV M G P+ +YS+AISACGRGGR +EA L+ +M++ G+ P+++ Y + ++AC GG A LL ++ G+ +VI+++++I+ C + G+W+ AL LR+M+ + R+ P+ I++NAAI ACA+GG +E LL M + D +Y++++D ++ + + + +L +M G P+ ISY A+ A K G ++ A LL M AG+ +W + ++Y+ + C K W +A LL M GL P+ CY + AC + + LL M G+ P + Y+ + A + + A+ + +M A + P+ + ++ I A +GG ++ V L E
Sbjct: 1 MKKMLYDSRSGDWRKALRAVDGMKKL---PGWLIDQKSYCMAITACGRGGQWEQAVRLLREMPTEGAAPDLSAYSAVIDACAKGGQWKMAVFFLMEMPTKGIAPDARSYGAAINACARGGRWKIALDLLREMLAR-DVTPNVIIYNSAINSCAKAGQWEIAVSLVKEMATVGLAPDVISYSSAISACGRGGRWEEALELFEDMRTSGVAPDVITYGSAIAACANGGRWKEAVSLLRKIPTVGLTPNVISYSSVITACAKEGQWKIALDLLREMKAM-RLAPNIITYNAAIDACAKGGRWKEAIDLLREMPTVGLPPDVVSYSSVIDACSKGDRWKEAIDILREMPTVG--LSPNAISYNSAIDACAKGGQWKEAKGLLREMPTAGVTQRVIGYNSAIAACAKGEQWKEALALLREMPTVGLHTTVFSYSSAIDACGKGNLWIKAKELLREMATVGLAPNEVCYNSAIDACGRGDQWEEAVDLLREMPTVGLSPDVFSYSSAIAACAKGDQWKEALAVLKEMSAAGLAPDLICYNSAIDACSKGGRWKMAVALLGE 548 HSP 4 Score: 210.69 bits (535), Expect = 4.180e-54 Identity = 146/527 (27.70%), Postives = 234/527 (44.40%), Query Frame = 0
Query: 221 QARAFCFSLLE----GSRPLSVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDR----------------------------------VEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEKTGRWPQALHLLHSMRRRGLTPDSTCYTVVVKACA 708
Q + F L+E G P SY + R G W+ A+ + + R + I A+AG++E A++ + +GL P ++ ++ GR +W L+E M GV P+ TY + A W E + +L N + ++ + WK A L++EM+ + P+ +TY AAI AC + A+ L+ M G P+ +YS+ I AC +G R EA + EM + GL PN + YN+ + AC +GG A LL EM AG+ + VI +N+ I+ C + +W+ AL LR+M T+ + P+ + +N+AI AC RG EE LL M + D +Y++ + + + + L VL +M G PD+I Y A+ A K G ++ A LL M+ AG+ N +Y+ + C + G+W + + LL MR G+ PD Y ++ CA
Sbjct: 83 QWKMAVFFLMEMPTKGIAP-DARSYGAAINACARGGRWKIALDLLREMLARDVTPNVIIYNSAINSCAKAGQWEIAVSLVKEMATVGLAP-DVISYSSAISACGRGGRWEEALELFEDMRTSGVAPDVITYGSAIAACANGGRWKEAVSLLRKIPTVGLTPNVISYSSVITACAKEGQWKIALDLLREMKAM-RLAPNIITYNAAIDACAKGGRWKEAIDLLREMPTVGLPPDVVSYSSVIDACSKGDRWKEAIDILREMPTVGLSPNAISYNSAIDACAKGGQWKEAKGLLREMPTAGVTQRVIGYNSAIAACAKGEQWKEALALLREMPTVGLHTTVFSYSSAIDACGKGNLWIKAKELLREMATVGLAPNEVCYNSAIDACGRGDQWEEAVDLLREMPTVGLSPDVFSYSSAIAACAKGDQWKEALAVLKEMSAAG--LAPDLICYNSAIDACSKGGRWKMAVALLGEMRAAGLTPNIISYSSAIDACVRGGQWKEGIALLEEMRGSGVVPDVITYHALMVTCA 604 HSP 5 Score: 177.178 bits (448), Expect = 7.756e-43 Identity = 121/438 (27.63%), Postives = 209/438 (47.72%), Query Frame = 0
Query: 238 VHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEMRERWHITPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACARGGAVEEGRRLLGRMKAQDVDRDTCTYNTLMDGYTRAGSFPDVLHVLDQMGKEGGRARPDVISYTQALSAAEKVGDYEAADRLLAAMKVAGIRWNQYTYAVVMALCEK 674
V SY + + GR G WE A+ +FE +R + I A G+++ A++ K +GL P+++ ++ + + QW L +M AM + PN TY + A W E + +L + V ++ + WK A +++EM ++P+ ++Y +AI AC + A L+ M G Y++AI+AC +G + EA L EM + GL + Y++ + AC +G A +LL EM G+ + + +N+ I C +WE A+ LR+M T+ + PD S+++AIAACA+G +E +L M A + D YN+ +D ++ G + + +L +M G P++ISY+ A+ A + G ++ LL M+ +G+ + TY +M C K
Sbjct: 173 VISYSSAISACGRGGRWEEALELFEDMRTSGVAPDVITYGSAIAACANGGRWKEAVSLLRKIPTVGL-TPNVISYSSVITACAKEGQWKIALDLLREMKAMRLAPNIITYNAAIDACAKGGRWKEAIDLLREMPTVGLPPDVVSYSSVIDACSKGDRWKEAIDILREMPTV-GLSPNAISYNSAIDACAKGGQWKEAKGLLREMPTAGVTQRVIGYNSAIAACAKGEQWKEALALLREMPTVGLHTTVFSYSSAIDACGKGNLWIKAKELLREMATVGLAPNEVCYNSAIDACGRGDQWEEAVDLLREMPTVG-LSPDVFSYSSAIAACAKGDQWKEALAVLKEMSAAGLAPDLICYNSAIDACSKGGRWKMAVALLGEMRAAG--LTPNIISYSSAIDACVRGGQWKEGIALLEEMRGSGVVPDVITYHALMVTCAK 605 HSP 6 Score: 141.354 bits (355), Expect = 4.799e-31 Identity = 93/366 (25.41%), Postives = 166/366 (45.36%), Query Frame = 0
Query: 237 SVHSYLTLMKNLGRAGDWEGAVGIFEALRRRHQGVGGKEFMKLIRVLARAGKYEAAMAYWDKAEALGLGPPSILDFNKLFDILGRAKQWPRCRSLYEKMFAMGVRPNKSTYQVLAHSAAVSAPWSEVLRILDAATLASCASNYV-FTMALKGLKVRRNWKAAWALIQEM--------------------------------RERWHI--TPDTVTYTAAISACERSSNCTVALSLVALMEVDGAHPNQYTYSAAISACGRGGRLDEAKRLWGEMKSRGLRPNLVVYNTMLSACERGGDVDAAFDLLEEMRAAGIDRDVITWNTLISVCKERGEWERALGCLRDMQTLDRVEPDTISFNAAIAACAR 567
+V SY +++ + G W+ A+ + ++ + I A+ G+++ A+ + +GL PP ++ ++ + D + +W + +M +G+ PN +Y + A W E +L A + + A+ WK A AL++EM RE + P+ V Y +AI AC R A+ L+ M G P+ ++YS+AI+AC +G + EA + EM + GL P+L+ YN+ + AC +GG A LL EMRAAG+ ++I++++ I C G+W+ + L +M+ V PD I+++A + CA+
Sbjct: 242 NVISYSSVITACAKEGQWKIALDLLREMKAMRLAPNIITYNAAIDACAKGGRWKEAIDLLREMPTVGL-PPDVVSYSSVIDACSKGDRWKEAIDILREMPTVGLSPNAISYNSAIDACAKGGQWKEAKGLLREMPTAGVTQRVIGYNSAIAACAKGEQWKEALALLREMPTVGLHTTVFSYSSAIDACGKGNLWIKAKELLREMATVGLAPNEVCYNSAIDACGRGDQWEEAVDLLREMPTVGLSPDVFSYSSAIAACAKGDQWKEALAVLKEMSAAGLAPDLICYNSAIDACSKGGRWKMAVALLGEMRAAGLTPNIISYSSAIDACVRGGQWKEGIALLEEMRG-SGVVPDVITYHALMVTCAK 605 The following BLAST results are available for this feature:
BLAST of EWM21396.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31) Total hits: 10
Relationships
This CDS is a part of the following mRNA feature(s):
Sequences
Synonyms
Publications
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