EWM21472.1, cds9225 (CDS) Nannochloropsis gaditana

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|585101702|gb|EWM21472.1| (glucose-methanol-choline oxidoreductase [Nannochloropsis gaditana])

HSP 1 Score: 1774.6 bits (4595), Expect = 0.000e+0
Identity = 862/862 (100.00%), Postives = 862/862 (100.00%), Query Frame = 0

Query:    1 MAFFVIIVAVALLVLLSRVVYHFLCRRPGLKDDWPCVPDCRVQERVGKWPTLPRGRLKYITATEFRTLCALCDTVLPAFEAEDVPKAIRAYLMELVGSDDLFERLSTDMSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGGTWYGWGTGFSSMPFRQREAVFGRLLTSIIEPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRDSIPPSCVGGGTDVRELSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCEDCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGGAIETPPSHAGLAGVIAPYGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGEPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRPHRDEASGTTRGGKGMSEYLKYVRKMGTPVGNTALFSAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKLAVKIKGPDAEKAISVKYCVPSTEEAIGNVSYPEGPTLKGEYCAW 862
            MAFFVIIVAVALLVLLSRVVYHFLCRRPGLKDDWPCVPDCRVQERVGKWPTLPRGRLKYITATEFRTLCALCDTVLPAFEAEDVPKAIRAYLMELVGSDDLFERLSTDMSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGGTWYGWGTGFSSMPFRQREAVFGRLLTSIIEPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRDSIPPSCVGGGTDVRELSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCEDCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGGAIETPPSHAGLAGVIAPYGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGEPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRPHRDEASGTTRGGKGMSEYLKYVRKMGTPVGNTALFSAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKLAVKIKGPDAEKAISVKYCVPSTEEAIGNVSYPEGPTLKGEYCAW
Sbjct:    1 MAFFVIIVAVALLVLLSRVVYHFLCRRPGLKDDWPCVPDCRVQERVGKWPTLPRGRLKYITATEFRTLCALCDTVLPAFEAEDVPKAIRAYLMELVGSDDLFERLSTDMSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGGTWYGWGTGFSSMPFRQREAVFGRLLTSIIEPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRDSIPPSCVGGGTDVRELSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCEDCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGGAIETPPSHAGLAGVIAPYGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGEPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRPHRDEASGTTRGGKGMSEYLKYVRKMGTPVGNTALFSAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKLAVKIKGPDAEKAISVKYCVPSTEEAIGNVSYPEGPTLKGEYCAW 862          

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|553194567|ref|XP_005855990.1| (glucose-methanol-choline oxidoreductase, partial [Nannochloropsis gaditana CCMP526] >gi|422293074|gb|EKU20375.1| glucose-methanol-choline oxidoreductase, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 645.966 bits (1665), Expect = 0.000e+0
Identity = 311/313 (99.36%), Postives = 312/313 (99.68%), Query Frame = 0

Query:  305 VLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCEDCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGG 617
            VLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGC+DCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWA HAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGG
Sbjct:    1 VLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCQDCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWADHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGG 313          

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|1035905028|gb|OAY34626.1| (hypothetical protein MANES_12G034700 [Manihot esculenta])

HSP 1 Score: 315.464 bits (807), Expect = 8.892e-92
Identity = 234/791 (29.58%), Postives = 371/791 (46.90%), Query Frame = 0

Query:   60 ITATEFRTLCALCDTVLPAFEAEDVPKAIRAYLMELVGSDDLFERLSTDMSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGGTWYGWG----TGFSSMPFRQREAVFGRL-LTSIIEPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRD--SIPPSCVGGGTDVRE--------LSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCEDCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDND-----AVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWG--GAIETPPSHAGLAGVIAPYGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGEPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRPHRDEASGTTRGGKGMSEYLKYVRKMGTPVGN----TALFSAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKLAVKIKG 824
            +++ + +TL A+C+  +P   A+D+ K I              ++L    S+Y     +  +P  V +++      +A  IV+++L  L   LG   L G   + W       FS +   +RE +  +    +   P+R + + +K  +  ++F+  D           NP+W A+GY   TR+++R     +PL+K  +     D  ++ PS +  G  V E        + CDVVI+GSG GGG AA VL  +G KV+VLEKG YY   D++   E  S   L E SG++      + ILAGS VGGG+ +NW A  RTP+ V R+W   H +  FG ++Y  A+DAV  R+ +    S    +            N+ L +G + +G +   VPRN      C  C  GC  G K+ T  T+L DA + G   +++    +K +  D N+      +GVIA           R S+              T+  Y L V A    I + GSL +P +++ SGL +  IGR+L LHPV    G FP   SD    +  G  ++  +   + +G      IETP         ++P+ +  + +  +      A    + RD      +V      + ++ + L + D+ NL+  +   L++++  GA         + TYR    + +      K + E+L  +   G P       T LFSAHQM SCRMG+TEE G V   GE+WE + LYV D SV PT++G+NPM+TI+S ++ +SKK+A  + G
Sbjct:   24 LSSGQIQTLAAMCEAFVPPLPADDLSKEIPV------------DKLQAVRSFYRASGSEPPIPDEVAELLVTTGLKEAVLIVKLILKVLSFRLGTLLLCGFMCFDWNWPFMHKFSEISPEKREEILKKWSRETFFMPLRIVFLAVKIFSLYVFFSRTDE-------NLQNPAWEAIGYNKDTREKLRKSKEERPLQKGIIELANEDDSTLTPSLIQKGLQVSEDPEHNTCKIKCDVVIIGSGCGGGVAAAVLANSGQKVLVLEKGNYYVPADYSSL-EWPSMAELYESSGFLSTLNGRIMILAGSTVGGGSAINWSACIRTPDSVLRDWRVDHKIPLFGSSDYQNAMDAVCNRIGVTDNCSKEGFQ------------NQVLRKGCENIGLQVGSVPRNSSEDHYCGSCNFGCRTGEKKGTDSTWLVDAVSRGAV-ILSGCEAEKFMLEDSNNGSRKNCLGVIA-----------RASN--------------TNITYILQVEAR-VTISACGSLLTPPLMISSGLVNPNIGRNLHLHPVIMAWGYFPEHESDFKGKMYEGGIITS-IHKVVSEGSKVCAIIETPALGPASFAALSPWISGQDLKDKMVKYPRTAHLFALIRDRGSGEVKV------KGRITHRLDQLDKDNLRIGLRQALRILVGAGAV-------EVGTYRSDGQKIACKGIKEKDLEEFLDTITVPGGPKSKEQNWTTLFSAHQMSSCRMGATEEEGAVDENGESWEAKNLYVCDGSVLPTAIGVNPMITIQSTAYCISKKIAESLIG 741          

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|1013938384|gb|KYQ92076.1| (glucose-methanol-choline oxidoreductase [Dictyostelium lacteum])

HSP 1 Score: 308.145 bits (788), Expect = 2.999e-89
Identity = 227/799 (28.41%), Postives = 388/799 (48.56%), Query Frame = 0

Query:   43 QERVGKWPTLPRGRLKYITATEFRTLCALCDTV----LPAFEAEDVPKAIRAYLMELVGSDDLFERLSTDMSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTG--GTWYGWGTGFSSMPFRQREAVFGRLLTSII----EPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRDSIPPSCVGGGTDVRELSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCE--DCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGH-----RPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMK---DGWGGAIETPPSHAGLAGVIAP--YGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGE-PQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRPHRDEASGTTRGGKGMSEYLKYVRKMGTPVGNTALFSAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKL 818
            Q+  G +  LP G     T  E++TL  + DT+    L  +E  D+P  + A+            +L+    YY R   D D+  +   ++            R  +  L+  L LF  +G      G  T F  +P + R+ +   + +S      +  R +  L+  +     FA   T  S      TNP+W ALGYQ                R P L  +  +    S +   +D R  S DVV++GSGAGGG  A +L  AG KV++LEKGGY S  +   + E ++   L E++G + + +LSV+ILAGSC+GGGTT+NW AS RTP+H+  EW  K     F   ++ +AL+ ++ R+N+NT++S ++              N+ L +G   +      + RNV+ C+   C  C  GC   +KQS+  T+LEDA   G  +++    V+++                  +R+   + SD + +    H        P  S RY++ V A+  ++ SAG++H+PA+LLRS +K++ IG +  LHPV  V G++     D  + V  G  ++      MK     +G  +E P +H GL+  +A   +  +F+++  +     + V I I RD +    ++ +D D   P++ Y L++KD  N+   +   ++ + + GA   L     +  ++   D+          +++Y++ ++ +       ++ SAHQM SCRMGS+     V   GE+W++++L++ D SVFP++LG+NPM+TI + S++++K +
Sbjct:   12 QDIKGSFHLLPTG---IFTLKEYQTLQVIIDTLISNDLSEYEQTDIPSNLSAH------------KLTELKEYYHRTGTDLDLAYQFMKLI-------IMTKTRSQISDLKTLLSLFSTSGFGAILTGSITNFCFLPLKTRQKILSSMKSSSNGTRRQAYRAIVPLVFTL-----FATVITTHS-----ETNPNWEALGYQ----------------RPPPLEQIPGEE-KLSFITVNSD-RSFSTDVVVIGSGAGGGVTASLLAKAGYKVLILEKGGYLSPNNMT-WKESEAFPQLYEQAGTLTSDDLSVNILAGSCLGGGTTVNWTASVRTPDHILDEW-RKDCPNTFANDKFQEALNTISERINVNTQYSTQSTA------------NQLLKKGLDDLQLESSVIARNVKDCDTTQCGFCSMGCRTKSKQSSTVTYLEDACADGA-QIITNCFVEEI-----------------TKRMEPSKGSDPQQQMECVHGVVGTVQAPDGSGRYRIFVKAN-IIVASAGAIHTPALLLRSRIKNNNIGSNFYLHPVCPVIGMY-----DQQVEVWKGPPMTVVSKAHMKTPTSNYGTILEVPNAHIGLSLAVASCQWAGSFDFKTLIQSIDRWNVYIPILRDST--PGKIKLDKDQRTPKIIYKLSEKDWKNMMPGIESSIRALHSTGAVKILLPCPGLPVFQSSHDD----------INQYIQTIKSLKYKPNGCSIVSAHQMGSCRMGSSRSNSVVNEQGESWDLKRLFISDGSVFPSALGVNPMLTIYATSYIIAKNI 710          

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|495183075|ref|WP_007907865.1| (glucose-methanol-choline oxidoreductase [Ktedonobacter racemifer] >gi|297556602|gb|EFH90466.1| glucose-methanol-choline oxidoreductase [Ktedonobacter racemifer DSM 44963])

HSP 1 Score: 303.908 bits (777), Expect = 4.286e-88
Identity = 234/760 (30.79%), Postives = 367/760 (48.29%), Query Frame = 0

Query:   59 YITATEFRTLCALCDTVLPAFEAEDVPKAIRAYLMELVGSDDLFERLSTDMSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGGTWYGWGTGFSSMPFRQREAVFGRLLTSIIEPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRDSIPPSCVGGGTDVRELSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTC-FGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCED-CSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGGAIETPPSHAGLAGVIAPYGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGEPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRPHRDEASGTTRGGKG------MSEYLKYVRKMGTPVGNTALFSAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESI 810
            ++ + EF  L A+C+T+ P+ E    P A         G+ +       + +YY R A D D+   + + + ++  P+A+   R +L  +  P+    L G         F  +   +R      +  S +  +R     +K +AG IYF+A    P+  G    NP+W  L Y         +    +PL                     T+   L  D +++GSGAGGG AA  L  AG  V+VLEKGGY +E +F    E  +   L    G + +++L V ILAGS +GGGT +NW  SFRTP  V  EW    GL   F  A+Y ++  AV +R+ +NT +S              N+ N  L+ G KA+G     +PRN  GC+  C  C  GC YGAKQST++T+L+DA + G  +++     +KVL  ++    GV+ATV  +            GR              Y+L + A   V+++AG+L++PA+LLRSGL +  IG+HL LHP + +AGL+P +      GV    Y   F  G +   +G  +E PP+H GL G+  P+ +A  YR  +A   H A  IV+SRD+   +  V +D  GEP ++Y+++  D+ +L + +    ++  A GA          R      ++ +G  RG  G      + E+ + + + G        F+AHQM +CRMG+  +       GE + V+ L+V D S+FP + G+NPM++I ++
Sbjct:   18 WLASEEFAILEAVCETLFPSLE----PPA---------GNSE------AEQAYYRRSAHDLDLAHLIAETLAQE-PPEAQAEFRQLLALMANPVSGLLLAGS-----AKSFLELSAAKRARYLLAMANSPLGQLRQGYQAMKRLAGFIYFSA----PTEEG---RNPNWEVLDYTPAPPPPADAPQPIRPL-------------------AITEDTTLDADAIVIGSGAGGGVAAAELALAGKSVIVLEKGGYNNEANFT-LQEAQATPELYLNHGLLTSKDLGVVILAGSTLGGGTVVNWTTSFRTPIDVLEEWETISGLRGRFTNAQYQESFAAVEQRIQVNTENSAH------------NRQNRLLYDGCKALGYHTEEIPRNAVGCDQRCGTCGYGCRYGAKQSTMKTYLQDAFDHGT-RIIVNCQANKVLM-ENGQVQGVLATVLNKE----------AGRT-------------YELTLHA-KTVVVAAGTLNTPAILLRSGLTNKHIGQHLHLHPTTTLAGLYPDKVYPWQ-GVMQSAYSDQF--GRLDGNYGYKLEVPPAHPGLLGLATPWFSAREYREQMAQAAHIATIIVLSRDKGEGS--VTLDRHGEPIINYVVSVYDRKHLMHGLRQSARIHFAAGA----------REIVTLHNQRTGVKRGSDGSVSAQDLREFDRAIERHGMGPNRNMTFTAHQMGTCRMGNDPKTSVTNEHGEVYGVKGLFVADGSLFPAASGVNPMLSIMAL 672          

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|1035404178|ref|XP_008447391.2| (PREDICTED: LOW QUALITY PROTEIN: long-chain-alcohol oxidase FAO2-like [Cucumis melo])

HSP 1 Score: 304.294 bits (778), Expect = 9.457e-88
Identity = 242/768 (31.51%), Postives = 352/768 (45.83%), Query Frame = 0

Query:   86 KAIRAYLMELVGSDDLFERLSTD---MSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGGTWYGWG----TGFSSMPFRQREAVFGRLLTS---IIEPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRD--SIPPSCVGGGTDVRE---------LSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCEDCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDK-VLRNDDNDA-----VGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGGAI-ETPPSHAGLAGVIAPYGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGEPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRP--HRDEASGTTRGGKGMSEYLKYVRKMGTPVGN----TALFSAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKLA 819
            +A+ A+   L+    LF     D   +S+Y   A  A +P  V D++ E   P A  +V++VL  L   +G   L G   + W       FS +   +RE +      +       +R   ++IK      +F+  D       GT  N +W A+GY+  TR ++      +PL +  +     D  ++  S    G +V E         + CDVVIVGSG GGG AA VL  +GLKVVVLEKG Y+  ED++   E  S   L E  G     +  V +LAG+ VGGG+ +NW AS RTP+ V  EW   H +  +G  +Y  A+DAV  R+ +      R   V S         N+ L +G + +G +   V RN      C  C  GC  G K+ T  T+L DA   G   L    H DK +L N +N +     +GVIAT                          P + +  + +     A I + GSL +P +L+ SGL++  IG +L LHPVS V G FP E SD    +  G  ++       K     AI ETP         + P+ +    +  +      A    + RD+     +V      +  V Y L + D+ NL+  +   L+++IA GA         + TYR    R    GT +  + + E+L  V  +G P       T   SAHQM SCRMGST+E G V   GE+WE E L+V D SV PT++G+NPM+TI+S ++ +SKK+A
Sbjct:   29 EAMAAFCEALIPPLPLFSETPLDQFLLSFYQSSAFQAPIPDEVADLLVERANPKAVFLVKLVLRLLSFRIGTLLLCGNVCFDWRWPFFLKFSEISLEKREQILKNWSMAHHKYTVLLRMTFMIIKIFCCFKFFSRIDE------GTK-NVAWKAMGYEVDTRKQLNHTQKERPLERGMIETGNEDDSTLFRSLSQKGLEVIEDKKHGNAYKIKCDVVIVGSGCGGGVAAAVLAKSGLKVVVLEKGNYFVAEDYSSL-EGPSMNELYESGGLKSTLDGKVMLLAGTTVGGGSAINWSASIRTPKSVLDEWSTDHKIPLYGSIDYQLAMDAVCDRIGVT-----RGCRVESF-------QNQVLRKGCENLGLKVDSVSRNSSEDHYCGSCCYGCRVGDKKGTDTTWLVDAVKCGAVILTG-CHADKFILENTNNTSKKXKCIGVIAT--------------------------PLSKNVTKKLHIEAKATISACGSLLTPPLLIASGLRNKNIGTNLHLHPVSLVWGYFPEELSDFKGKIYEGGLITCLHNVVSKYSNSHAIIETPAVGPASFAALFPWTSGREMKDAMVKYSRTAHLFALVRDQGAGEIKV------KANVKYRLNQTDKENLKIGLRQALRILIAAGAT-------EIGTYRSDGQRIRCQGTKK--EDLEEFLDSVEAVGGPWSRQKYWTMYCSAHQMSSCRMGSTKEEGAVDENGESWEAEGLFVCDGSVLPTAVGVNPMITIQSTAYCISKKIA 734          

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|658057107|ref|XP_008364320.1| (PREDICTED: long-chain-alcohol oxidase FAO2-like [Malus domestica])

HSP 1 Score: 303.908 bits (777), Expect = 1.574e-87
Identity = 229/780 (29.36%), Postives = 359/780 (46.03%), Query Frame = 0

Query:   60 ITATEFRTLCALCDTVLPAFEAEDVPKAIRAYLMELVGSDDLFERLSTDMSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGGTWYGWG----TGFSSMPFRQREAVFGRLLT-SIIEPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRD--SIPPSCVGGGTDVRE--------LSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCEDCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGGAI-ETPPSHAGLAGVIAPYGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGEPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRPHRDEASGTTRGGKGMSEYLKYVRKMGTPVGNTALF----SAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKLA 819
             ++ + ++L A+C+ ++P    E++P             DD      +  SY+T       +P  V +++ +  +P+A   +R+ L  L + LG F L G   + W       FS     +RE V  +     ++  IR + +++K      +F+  D           NP+W A+G+    ++++      +PL K  +  +     ++  S    G +V +        ++CDVVIVGSG GGG AA VL  +G KVVVLEKG Y+  +D++   E  S   L E  G M + +  V ILAGS VGGG+ +NW AS RTP +V REW   H +  +   +Y  A+D V +R+++    +  N +            N+ L +G + +G +   VPRN      C  C  GCP G K+ T  T+L DA   G   L      +K +   DND                GR     G   +      S + + +L + A    I + GSL +P +++ SGLK+  IGR+L LHPV    G FP   S+       G  ++       +     AI ET          ++P+ +  + +  +      A    + RD S      V+  +G  +VHY L + D+ NL   +   L+++IA GA         + TYR             + + E+L  V     P     L+    SAHQM SCRMG+TEE G V   GE+WE + L+V DAS+ PT++G+NPM+TIES ++ +SKK+A
Sbjct:   25 FSSAQMQSLAAICEALIPPLSLEEIP-------------DDHQALHYSSSSYHTASGSQPPIPDEVAEMIVKRAQPEAVDAMRLFLKMLSLRLGTFLLCGKICFDWKWPFIHKFSEXSLDKREQVLNKWSRGKLVITIRLMFMVVKMFCFYTFFSRIDE-------NSRNPAWKAIGFDLDKKEKLIKTPKERPLDKGIIETVYESDSTLVQSLTKKGLEVADDAANNIHRINCDVVIVGSGCGGGVAAAVLAQSGQKVVVLEKGNYFVPKDYSSL-EGPSMSELYESGGIMASVDAMVMILAGSTVGGGSAVNWSASIRTPHNVLREWSVDHKIPLYASEDYQSAMDIVCKRISVTEGCTEENFQ------------NQILRKGCENLGLKVESVPRNSSADHYCGFCNYGCPTGDKKGTDSTWLVDAVECGAVILTG-CKAEKFILESDNDG---------------GRRKRCLGVQATSX----SKNIKRKLKIEAK-VTISACGSLLTPPLMISSGLKNRNIGRNLHLHPVLLSWGYFPQHESEFXXKKYEGGIITSIHKVVSETANARAIIETAVLGPASFATLSPWISRLDMKDKMEKYARTANLFALVRDXSSG----VVKREG--RVHYTLDQLDKENLLAGLRQALRILIAAGAV-------EVGTYRNDGQRIKCKGVKEEDLEEFLDTVVTSAGPSAREELWTIYSSAHQMGSCRMGATEEEGGVDENGESWEAKGLFVCDASLLPTAVGVNPMITIESTAYCISKKIA 737          

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|1173961016|gb|OQS07090.1| (hypothetical protein THRCLA_00889 [Thraustotheca clavata])

HSP 1 Score: 301.982 bits (772), Expect = 6.123e-87
Identity = 245/780 (31.41%), Postives = 375/780 (48.08%), Query Frame = 0

Query:   60 ITATEFRTLCALCDTV---LPAFEAEDVPKAIRAYLME-----LVGSDDLFERLSTDMSYYTRGALDADVPARVG-DIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGGTWYGWGTGFSSMPFRQREAVFGRLLTSIIEPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRDSIPPSCVGGGTDVRELSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYY--SEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCE-DCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGGAIETPPSHAGLAGVIAPYGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGEPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRPHR----DEASGTTRGGKGMSEYLKYVRKMGTPVGNTALFSAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKLAVKIK 823
            ++  +  TL A+ DT    L + + ED+ +   A L E     +   +D+ + L+TD       +LD    A+     + +D + D  K+  +    L    G + LTG       T F  +P  +RE     +  S    +RG+    K +     F  + T          NP+W A+G+     +R  +        KPQ      D    +   G  +   +  DVVIVGSGAGGG  A  L  AG +V+VLEKG YY   E+DF    EL+S R   E+   + +++ S+ + AGS  GGGTT+NW  SFR P  VR+EW  KH L  F   +++QA D +  RL ++  H            L +N  N+ L  G   +G     + +   G E  C  C  GCP   KQ T  ++L DA+  G  K +    VDKV  ++D  A GV  TV                            + + +LIV A   V+ SAGS+H+P++LLRSGLK+  IG++  +HPV  + G FP++T+    G  + T VS  +     +G+G ++E P    GL  V+ P+    +Y+  +    H +    + RD   + +R+V D  G+P+VH+ L  KD   +Q  +     +M++QGA   + +N S  +  P +    +E +      K   E++K +R  G     TALF+AHQM SCRMG+T  AG V   GETWE++ LYV DAS+FPT+ G+NPM T  +IS+ +++ +   ++
Sbjct:    3 LSDAQLETLSAIIDTFVSPLSSAQVEDITQEYFAQLKEHNANIIPSENDVKQFLTTDPK-----SLDLLTEAQTQIKFLPKDKQADLIKVFDL----LASGWGTYVLTGTR----STPFHQLPQSERETGLYNMAQSRFATVRGIFQAFKGLFVGGLFMKKQTVDGQ-----ANPTWAAMGFTGEPENRAPA----SAFVKPQFE----DVQAMAAALGENEPIVIETDVVIVGSGAGGGVVAAELAQAGYRVLVLEKGQYYYGPEDDF---NELESYRNKYEQGCLLTSEDNSLILFAGSTWGGGTTVNWSGSFRLPYEVRQEWAKKHNLPYFASPQFEQAFDRIYERLGISDAH------------LKLNCPNQILIDGCTKLGLPARKIEQTTGGAEHSCGFCTLGCPANEKQGTAVSWLRDAQAAGA-KFIQGCFVDKVTYSNDRVATGVEGTV---------------------------LNGKTKLIVRAT-TVVASAGSIHTPSLLLRSGLKNKNIGKNHRVHPVVTLNGFFPTKTTRPWSG-SILTSVSSALSNINGNGYGASLEVPVGLPGLVSVMLPWRNNVDYKTKMLQIPHLSTVFALVRDYD-SVSRIVNDATGQPRVHFQLGPKDAYAMQEGLVTSANIMLSQGA---VELNTSHHSLPPLKLTTPEELANPVECAK-TQEWIKQLRATGVAQNRTALFNAHQMGSCRMGATPAAGAVNPDGETWEIKGLYVADASLFPTASGVNPMTTTYAISYSVAQFMKANLE 706          

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|66801723|ref|XP_629786.1| (glucose-methanol-choline oxidoreductase [Dictyostelium discoideum AX4] >gi|60463184|gb|EAL61377.1| glucose-methanol-choline oxidoreductase [Dictyostelium discoideum AX4])

HSP 1 Score: 299.671 bits (766), Expect = 1.119e-85
Identity = 231/785 (29.43%), Postives = 380/785 (48.41%), Query Frame = 0

Query:   60 ITATEFRTLCALCDTVLPAFEAEDVPKAIRAYLMELVGSDDLFER---LSTDMSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGG--TWYGWGTGFSSMPFRQREAVFGRLLTSIIEPIRGLAI-LIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRDSIPPSCVGGGTDVRELSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCE--DCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAV------------GVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSAGSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDG--WGGAIETPPSHAGLAGVIAPY--GTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDG-EPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALA-LPVNESMRTYRPHRDEASGTTRGGKGMSEYLKYVRKMGTPVGNTALFSAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKL 818
             T TE++TL  +CDT+  + ++E     I   L +   + D   +   L+    Y+ R + D DV      ++       A K    +   L+  LGLF  TG      G  + F ++P + R+ V   ++ S   PIR  A   I  +A  IY++          G  +NP+W A+GY   T      + G + L   ++                T    L  DVV++GSGAGGG  A +L  AG KV+VLEKG Y S  +   + E ++   L E++G + + +LSV+ILAG+C+GGGTT+NW AS RTP+ +  EW  K     F   +++ A+D+V+ RLN+NT  +  +   A       N+NN+ L Q  + + A   P+PRN + C+   C  C  GC   +KQS++ T+LED  + G  +++     +++                     GVIA V                  P G +        Y++ + +H  V+ SAG+LH+P +LL+S +K+  IG +L LHPV  + G   ++  +   G  M      +       G   G  +ETP SH  L+G    +    +FN++  +    +Y+  I I RD      ++ +D DG  P++ Y L+ +D  ++  S+   L+ +I  GA  A +P++     ++P   +          + +Y+K ++  G     T + SAHQM SCRMGS++    V   GE+W+V++L++ D SV P+S+G+NPM+TI +IS +++ ++
Sbjct:   31 FTLTEYKTLTVICDTLF-SDDSEIALNEIENLLPKNSKNSDENHKEHQLNILKEYFKRKSSDLDVAGHFLKLIV------ATKSSSQI-SDLKTLLGLFSTTGAGIILTGQFSSFVNLPLKSRQQVL-TMMKSSSNPIRRQAFKAIVPLAISIYYSVIGD-----RGETSNPNWDAVGYD--TPPPPEQIPGEEKLSFIKI----------------TSETSLKADVVVIGSGAGGGVTAALLAEAGYKVIVLEKGSYISSNNMT-WKESEAFPLLYEQAGTLTSDDLSVNILAGNCLGGGTTVNWTASIRTPDIILEEW-RKQCPNAFDSIKFNNAMDSVSSRLNVNTIDTALDGTGAH------NRNNKLLEQALRELDADPQPIPRNTKNCDTTQCGNCSMGCRSKSKQSSMVTYLEDCCSKGG-QIITNCQAEEITTTTIPTNSTTTNGGGGQTVHGVIAYVST----------------PDGLK--------YRVFIKSH-IVVCSAGALHTPTLLLKSRIKNSNIGNNLYLHPVVPIIGEH-TQPVEVWKGPPMTVISKKYQKILPNSGKNTGSILETPNSHIALSGSNCAFMWDDSFNFKKRMLKARYYSGFISICRD--TVPGKIRLDKDGRSPKIQYTLSDRDWKSMLPSIEASLRALIKIGAIEASIPIS----GFKPGNQDT---------IEQYIKAIKSNGYKSNQTVILSAHQMGSCRMGSSKSNSVVNENGESWDVKRLFIADGSVLPSSVGVNPMITIYAISHIIANQI 733          

BLAST of EWM21472.1 vs. NCBI_GenBank
Match: gi|694415945|ref|XP_009336115.1| (PREDICTED: long-chain-alcohol oxidase FAO2-like [Pyrus x bretschneideri])

HSP 1 Score: 297.745 bits (761), Expect = 2.945e-85
Identity = 230/793 (29.00%), Postives = 364/793 (45.90%), Query Frame = 0

Query:   60 ITATEFRTLCALCDTVLPAFEAEDVPKAIRAYLMELVGSDDLFERLSTDMSYYTRGALDADVPARVGDIVEEDLRPDARKIVRMVLYALEMPLGLFFLTGGTWYGWG----TGFSSMPFRQREAVFGRLLT-SIIEPIRGLAILIKAMAGRIYFAARDTPPSTPGGTPTNPSWTALGYQDRTRDRVRSVMGGKPLRKPQLHPLRRD--SIPPSCVGGGTDVRE--------LSCDVVIVGSGAGGGTAAGVLTAAGLKVVVLEKGGYYSEEDFAGFGELDSERALCERSGWMGAQELSVSILAGSCVGGGTTLNWCASFRTPEHVRREWVDKHGLTCFGGAEYDQALDAVARRLNLNTRHSHRNPEVASVPSLVVNQNNERLWQGAKAVGARCLPVPRNVRGCEDCSGCERGCPYGAKQSTLRTFLEDAENTGNFKLVARAHVDKVLRNDDNDAVGVIATVHEERRLAEGRESDGEGRPPSGHRPGPSTSSRYQLIVWAHHAVILSA-GSLHSPAVLLRSGLKHHKIGRHLALHPVSGVAGLFPSETSDTHLGVGMGTYVSDFMGGEMKDGWGGAI-ETPPSHAGLAGVIAPYGTAFNYRMWVALKGHYAVSIVISRDESCAANRVVIDNDGEPQVHYLLTKKDQANLQYSVGVMLKMMIAQGAALALPVNESMRTYRPHRDEASGTTRGGKGMSEYLKYVRKMGTPVGNTALF----SAHQMCSCRMGSTEEAGPVQVTGETWEVEKLYVMDASVFPTSLGINPMVTIESISFMLSKKLAVKIKGPDAEKAI 831
             ++ + ++L A+C++++P    E++P   +A               S+  SY+T       +P  V +++ +   P+    +R+ L  L + LG F L G   + W       FS M   +RE V  +     ++  IR + +++K      +F+  D           NP+W A+G++   ++++      +PL K  +  +     ++  S    G +V +        + CDVVIVGSG GGG AA VL  +G KVVVLEKG Y+  +D++   E  S   L E  G M + +  V ILAGS VGGG+ +NW AS RTP +V REW   H +  +   +Y  A+D V +R+ +    +  N +            N+ L +G + +G +   VPRN      C  C  GCP G K+ T  T+L DA   G   L      +K +   DND                GR     G   +      S S   +  +     VI+SA GSL +P +++ SGLK+  IGR+L LHPV    G FP   S+       G  ++       +     AI ET          ++P+ +  + +  +      A    + RD S      V+  +G  ++HY L + D+ N+   +   L+++IA GA         + TYR             + + E+L  V     P     L+    SAHQM SCRMG+TEE G V   GE+WE + L+V DAS+ PT++G+NPM+T+ES ++ ++KK+A  +   D +K I
Sbjct:   25 FSSAQMQSLAAICESLIPPLPLEEIPDDHQA-----------LHSSSSSSSYHTASGSQPPIPDEVAEMIVKRALPEGVDAMRLFLKMLSLRLGTFLLCGKICFDWKWPFIHKFSEMSLDKREQVLNKWSRGKLVITIRLMFMVVKMCCFYTFFSRIDE-------NSRNPAWKAIGFELDKKEKLIKTPKERPLDKGIIETVYESDSTLVQSLTKKGLEVADDAENNIHRIKCDVVIVGSGCGGGVAAAVLAQSGQKVVVLEKGNYFVPKDYSSL-EGPSMSELYESGGIMSSVDAMVMILAGSTVGGGSAVNWSASIRTPNNVLREWSVDHKIPLYASEDYQSAMDIVCKRIGVTESCTKENFQ------------NQILRKGCENLGLKVESVPRNSSADHYCGFCNYGCPTGDKKGTDSTWLVDAVECGAVILTG-CKAEKFILEKDNDG---------------GRRKRCLGVQAT------SLSKNLKRKLEIEAKVIISACGSLLTPPLMVSSGLKNRNIGRNLHLHPVLLSWGYFPQHESEFKGKKYEGGIITSIHKVVSETANARAIIETAVLGPASFATLSPWISRLDMKDKMEKYARTANLFALVRDHSSG----VVKREG--RIHYTLDQLDKENILAGLRQALRILIAAGAV-------EVGTYRNDGQRLKCKGVKEEDLEEFLDTVVTSAGPSAREELWTIYSSAHQMGSCRMGATEEEGGVDENGESWEAKGLFVCDASLLPTAVGVNPMITVESTAYCIAKKIAQSL---DKQKYI 748