EWM21593.1, cds9085 (CDS) Nannochloropsis gaditana

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|553185155|ref|XP_005854208.1| (long-chain acyl-CoA ligase [Nannochloropsis gaditana CCMP526] >gi|422294847|gb|EKU22147.1| long-chain acyl-CoA ligase [Nannochloropsis gaditana CCMP526] >gi|585101848|gb|EWM21593.1| amp-dependent synthetase and ligase [Nannochloropsis gaditana])

HSP 1 Score: 1467.21 bits (3797), Expect = 0.000e+0
Identity = 707/707 (100.00%), Postives = 707/707 (100.00%), Query Frame = 0

Query:    1 MHRYKHFAIRGIYGHWGHQGQGYQIMSSSLRVTQSRSASHVPSSSTIPSFTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEVVRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLEQGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQANVASYPKSRAKETPDTQQPYEDALAA 707
            MHRYKHFAIRGIYGHWGHQGQGYQIMSSSLRVTQSRSASHVPSSSTIPSFTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEVVRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLEQGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQANVASYPKSRAKETPDTQQPYEDALAA
Sbjct:    1 MHRYKHFAIRGIYGHWGHQGQGYQIMSSSLRVTQSRSASHVPSSSTIPSFTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEVVRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLEQGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQANVASYPKSRAKETPDTQQPYEDALAA 707          

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|298714214|emb|CBJ27350.1| (long chain acyl-coA synthetase [Ectocarpus siliculosus])

HSP 1 Score: 446.047 bits (1146), Expect = 4.802e-144
Identity = 250/652 (38.34%), Postives = 375/652 (57.52%), Query Frame = 0

Query:   35 SRSASHVPSSSTIP---SFTTLVEMQQSVARTQGKKDFLG-YKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINM-QESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEVVRPEDMPNAL--SLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLEQG-FLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIY 678
            S++A+  P     P    + TLV+M     ++ G +   G  K    S ++W+TY+ F   V + +  L   GI  GD V  +S NR EWAV   A+ SLGA +VPMYE+QR  +  YIL DSGAK L V ++  Y +T HF        A  QG+  +V    Q +    S KD+                 +   A+    + +  P ++A LIYTSGTTG PKGV L+H N+ +NI G+  ++ P+D+ +    SL+FLPWAH  G   EL  +M+ GA + +++D+T +   + EV+P +L+AVP L+K+V D I  K+       ++L  +++ +A +R + +  G+ +   L+ Q+ +LD  VLSK+RD  GG +++S VGG+  ++ V  FFE  + I ILEGYGL+ETSP+++++ P+ + RRLGS G+ +    + ++  G  +P GEEGE+  SGP V +GY    E ++    ++DG+++FRTGDLG ++ G +L ITGR+KE YKLENGKYV    +E A+  S YI Q +++G + PFN AL+ P+W  + +W  SK       S    E+ +   V+  +   I L+  D  KKYEMP+ + LLEE FT EN M T K+S KRH VIK Y   L  +Y
Sbjct:   59 SKAAAASPPVHATPHYMDYDTLVDMHLITRQSFGNRPLFGTQKGDKDSPFEWLTYKEFGKQVDRCRAALAGLGITHGDTVGIISNNREEWAVCAYATYSLGAVFVPMYEEQRPEDWRYILEDSGAKCLFVATKGIYRETFHF--------AGVQGNVQNVFCFDQPAGQPGSFKDL-----------------IDTNAEEKVPAYIPDPSELATLIYTSGTTGKPKGVMLSHGNIISNIVGIRAIL-PDDLVSCHDRSLSFLPWAHCYGQTAELHALMAHGASMGISRDVTALLDQLAEVKPTLLYAVPQLFKRVCDAIHAKVAESSPAKQYLFKRSLQMAHRRRKHIEAGEPVGAALELQHRLLDQAVLSKIRDRFGGNLKVSFVGGAATNMEVLNFFE-NIDIKILEGYGLTETSPMVTVNTPEQKFRRLGSTGRCLADVNVKIMMGGEEMPAGEEGEVCVSGPNVMQGYNNLPEASSEVLFDLDGQRYFRTGDLGRMDDGTYLRITGRIKEQYKLENGKYVVPGPIEAAMTSSTYITQALVFGDNRPFNVALVFPDWNLVRSWAESKGRANDGTSLE--ELASMDVVKNLIAGEIALSL-DGFKKYEMPRTFELLEEGFTKENNMQTQKLSTKRHVVIKHYHDTLMHMY 680          

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|219113383|ref|XP_002186275.1| (long chain acyl-CoA synthetase [Phaeodactylum tricornutum CCAP 1055/1] >gi|209583125|gb|ACI65745.1| long chain acyl-CoA synthetase [Phaeodactylum tricornutum CCAP 1055/1] >gi|554888953|gb|AGZ03884.1| long-chain acyl-CoA synthetase 3 [Phaeodactylum tricornutum])

HSP 1 Score: 433.721 bits (1114), Expect = 4.473e-139
Identity = 256/651 (39.32%), Postives = 372/651 (57.14%), Query Frame = 0

Query:   44 SSTIPSFTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDV--DVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEV---VRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGL--QAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVV-RDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEM-DGK-KWFRTGDLGTL-EQGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQANV 683
            S  + +F TL EMQ    +   +K   G     S  Y+WI++  F+  V   + VLR+ G+   DKV  +S NRVEW     A+ SL A  VPMYE Q  ++  YIL DSGAK++   + + YD                      V+  Q    V +L+     D     P   + +    TA+ D  F   +  PDD+A LIYTSGTTG PKGVELTH N C+N++        VR     + +SLAFLPWAHS G   EL++ M+ G+ + +++ ++ I  D++ V+P +LFAVPTL+KK+YD + + + +     + L+  A+++  +R+ +  EG R +LG   + +  VLD +VL K+R   GG++R + V G+     V  F +  +GI + EGYGL+ETSP+++I+ P  + R++GSVG+A+ G  + +V  +G  +P GEEGEI  +GP + +GY    E TA       DGK + F TGDLG + E GFL +TGRLKELYKLENGKYVC   +E+ +  S +I QVVL GA+ PFN AL+VP W  + A        GL  S    E+  + +V+A +  +I + CK+ MKK+E+PQ W  +  PFT  N+MLTPKMSI+RH VI  Y   + A+YD  ++
Sbjct:   77 SDGLTAFETLHEMQTRSCQVFAEKRLFGTYSDASKAYEWISFAEFDQAVSVCRAVLRDLGVQEHDKVGLISNNRVEWVTIACAAYSLNANVVPMYEAQLPSDWTYILNDSGAKVVFCATTDIYD----------------------VVRSQVRINVPTLQANVCLDAPDGEPFAYSTLMHHATADVDGQFVK-LPTPDDLANLIYTSGTTGKPKGVELTHENFCSNVKAGCRSLGDVRKLIKDDDVSLAFLPWAHSYGQTAELWSSMAHGSSMGISRGVSHILEDLQLVQPTVLFAVPTLFKKIYDGVHNAMEAAPPLRKKLMKAALSLGRERVAV-NEGTRDALGFIERTKLGVLDKLVLGKIRARFGGKLRHAFVAGAACPPEVLSFMDD-VGIQVCEGYGLTETSPIITINTP--EYRKVGSVGKAIGGVTVHIVDEEGKELPIGEEGEICCTGPNIMRGYYGNAEATAEVITTAPDGKTRMFHTGDLGRMGEDGFLKVTGRLKELYKLENGKYVCPTPIEEGIGMSRFIAQVVLTGANQPFNVALVVPEWPAIRAEL------GLDDSVSDDELAMNEKVRALVDADIKIKCKN-MKKFEVPQNWAFV-APFTAANDMLTPKMSIRRHKVISTYNDIIAAMYDSESI 692          

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|502591048|ref|WP_012828673.1| (long-chain fatty acid--CoA ligase [Haliangium ochraceum] >gi|262080105|gb|ACY16074.1| AMP-dependent synthetase and ligase [Haliangium ochraceum DSM 14365])

HSP 1 Score: 415.616 bits (1067), Expect = 2.654e-133
Identity = 234/634 (36.91%), Postives = 356/634 (56.15%), Query Frame = 0

Query:   50 FTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEVVRPEDMP---NALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRD-GLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLEQ-GFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIY 678
            +T+LVEMQ+       K+   G K      Y+WITY  F  +V + +  L + G+  GD+VA +S NRVEWAVG  A+ SLG  +VPMYE Q   E  YI+ DS +K+ L  +   Y +    +                         V SL+    FD    A  + +  +    A+   +S    PDD+A L+YTSGTTG PKGV L+H N+C+N+      V P+  P     ++ +FLPWAH+ G   EL  ++S GA + LA+ + T+  +  EVRP +L AVP ++ ++YD ++ ++  + K  + L + A++V++KR E+   G   +L L  Q+++ + +V +K+++  GGR+R    GG+ +++ V  F +  LGI + EGYGL+ETSP+ +++RP    RR+G+VGQA+    + +  + G  +P   +GE+  SGP + +GY    E TA    E+DGK+ FRTGD+G L Q GFL+ITGR KE YKLENGKYV   ++E  L  S +I Q  +YG + PFN  LIVP++E +  W  S +  G+S       +     V A + E +  A   + K +E P++W LL E F+ EN ++TPKMS++R AV   Y   L+ +Y
Sbjct:    5 YTSLVEMQEDACERFAKRRVFGTKL--EGIYRWITYADFASLVNRFRAALAQLGVEAGDRVAVVSNNRVEWAVGAYATYSLGGHYVPMYEAQSPAERRYIIEDSNSKVALAATEAIYQELAGLIGE-----------------------VDSLEHAICFDASESAPHSYLHHLAVG-AENPVSSVHPSPDDLAGLLYTSGTTGKPKGVMLSHNNICSNLR-----VMPDLFPLSHEDVTCSFLPWAHAFGQTAELHGIISAGASMGLAESVQTLMENFLEVRPTVLLAVPRIFHRIYDGVKKRMADEPKLRQKLFEHALSVSQKRTEMRDHGGSSTL-LDIQHKLFERVVFAKIKERFGGRLRFVMSGGAALAVEVAEFID-NLGITVFEGYGLTETSPVATVNRPG--ARRIGTVGQAIPDVSIYICDEQGKLLPPETDGEVLISGPNIMQGYHGLPEATAEVMVEVDGKQAFRTGDMGRLSQDGFLSITGRFKEQYKLENGKYVVPTLLEDQLKLSGFITQAFIYGDNKPFNVCLIVPDFEAIQKWAKSDEGGGVSGDLSPEALCADARVHAKIGEELA-AYGAEFKHFEQPKRWKLLVEDFSPENGLMTPKMSVRRRAVTARYQDELDKLY 602          

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|499871872|ref|WP_011552606.1| (long-chain fatty acid--CoA ligase [Myxococcus xanthus] >gi|108462896|gb|ABF88081.1| putative long-chain-fatty-acid--CoA ligase [Myxococcus xanthus DK 1622] >gi|1089564839|emb|SDW94184.1| long-chain acyl-CoA synthetase [Myxococcus xanthus])

HSP 1 Score: 406.371 bits (1043), Expect = 9.271e-130
Identity = 244/639 (38.18%), Postives = 361/639 (56.49%), Query Frame = 0

Query:   47 IPSFTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEV--VRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLE-QGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQAN 682
            +P F TL+++ +    T G +D  G K   + ++ W TY  F +MV   +  L + G+G GD+VA +S NR+EWAVG  A+ +LG  +VPMYE Q++ E ++IL DSGAK++   + +   + +      S      Q + +    ++ S T S        D D  A + R      AE      S   +P D+A LIYTSGTTG PKGV L+H N+  N+  M EV  +  ED     SLAFLPWAH  G   EL  ++S GA +A+A+ +  I  ++ EV+P +LF+VP ++ ++YD +Q ++  +   +R++  + +AVA +R  +   GK   L L  Q+   D +V SK+R   GGR++ +  GGS +S  V  F +  LGI + EGYGL+ETSP+ + + P+   R++GSVG+A+ G  + +  D     E  +GEI   G  V  GY  K EE    F    G   FRTGD+G L+  G+L ITGR+KE YKLENGKYV    +E++L  S YI   +++G + P+N A+IV + + L  W   K   GL  +S   E+   PEV    RE +    +D +K YE PQ++LL+ E FTV N+MLTPKMS+KR  V+  Y  A+ A+Y + +
Sbjct:    3 LPQFQTLIDIFKRSTSTFGSRDLFGEKK--NGQWVWTTYSRFGEMVDDLRGGLAQLGVGAGDRVAVISNNRLEWAVGAYATYTLGGAYVPMYESQQVKELQFILNDSGAKVVFCATDDIAQRIQ------SVRAELPQLEHI----IRFSGTTS--------DTDSFATLLR----RGAETPTPLVSP--KPADLAGLIYTSGTTGQPKGVMLSHANIARNVSAMHEVFPMGTEDR----SLAFLPWAHVFGQTVELHALLSMGASMAIAEAVEKIIDNLSEVKPTLLFSVPRIFNRIYDGLQKRMAGEKAVTRFMFHRGLAVAAQRRALAEAGKSSGL-LDLQHAFFDKVVFSKVRARFGGRLKYAFSGGSAISKEVAEFID-NLGITVYEGYGLTETSPIATANFPN--NRKIGSVGKALPGIRVEI--DTAATGEATQGEIVVHGHNVMMGYYNKPEENEKVF---TGNGGFRTGDMGYLDPDGYLYITGRIKEQYKLENGKYVVPSPIEQSLALSTYIANALVHGMNKPYNVAIIVVDVDTLKKWATEK---GLDTTSMP-ELLKRPEVLQLYREQLNEFTRD-VKGYERPQRFLLISEDFTVANDMLTPKMSVKRRNVVARYNDAIEALYREGS 597          

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|1087649439|emb|SDD93266.1| (long-chain acyl-CoA synthetase [Myxococcus virescens])

HSP 1 Score: 405.601 bits (1041), Expect = 1.655e-129
Identity = 244/639 (38.18%), Postives = 361/639 (56.49%), Query Frame = 0

Query:   47 IPSFTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEV--VRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLE-QGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQAN 682
            +P F TL+++ +    T G +D  G K   + ++ W TY  F +MV   +  L + G+G GD+VA +S NR+EWAVG  A+ +LG  +VPMYE Q++ E ++IL DSG+K++   + +   + +            A+   L  I ++ S T S        D D  A + R      AE      S   +P D+A LIYTSGTTG PKGV L+H N+  N+  M EV  +  ED     SLAFLPWAH  G   EL  ++S GA +A+A+ +  I  ++ EV+P +LF+VP ++ ++YD +Q ++  +   +R++  + +AVA +R  +   GK   L L  Q+   D +V SK+R   GGR++ +  GGS +S  V  F +  LGI + EGYGL+ETSP+ + + P+   R++GSVG+A+ G  + +  D     E  +GEI   G  V  GY  K EE    F    G   FRTGD+G L+  G+L ITGR+KE YKLENGKYV    +E++L  S YI   +++G + P+N A+IV + + L  W   K   GL  +S   E+   PEV    RE +    +D +K YE PQ++LL+ E FTV N+MLTPKMS+KR  V+  Y  A+ A+Y + +
Sbjct:    3 LPQFQTLIDIFKRSTSTFGSRDLFGEKK--NGQWVWTTYSRFGEMVDDLRGGLAQLGVGAGDRVAVISNNRLEWAVGAYATYTLGGAYVPMYESQQVKELQFILNDSGSKVVFCATDDIAQRIQ---------AVRAELPHLEHI-IRFSGTTS--------DTDSFATLLR----RGAETPTPLVSP--KPTDLAGLIYTSGTTGQPKGVMLSHSNIARNVSAMHEVFPMGTEDR----SLAFLPWAHVFGQTVELHALLSMGASMAIAEAVEKIIDNLSEVKPTLLFSVPRIFNRIYDGLQKRMAGEKAVTRFMFHRGLAVAAQRRALAEAGKSSGL-LDLQHAFFDKVVFSKVRARFGGRLKYAFSGGSAISKEVAEFID-NLGITVYEGYGLTETSPIATANFPN--NRKIGSVGKALPGVRVEI--DTAATGEATQGEIVVHGHNVMMGYYNKPEENEKVF---TGNGGFRTGDMGYLDPDGYLYITGRIKEQYKLENGKYVVPSPIEQSLALSTYIANALVHGMNKPYNVAIIVVDVDTLKKWAAEK---GLDTTSMP-ELLKRPEVLQLYREQLNEFTRD-VKGYERPQRFLLISEDFTVANDMLTPKMSVKRRNVVARYNDAIEALYREGS 597          

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|488715160|ref|WP_002639036.1| (long-chain fatty acid--CoA ligase [Myxococcus hansupus] >gi|891162908|gb|AKQ67593.1| Long-chain-fatty-acid--CoA ligase [Myxococcus hansupus])

HSP 1 Score: 405.216 bits (1040), Expect = 2.187e-129
Identity = 243/639 (38.03%), Postives = 361/639 (56.49%), Query Frame = 0

Query:   47 IPSFTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEV--VRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLEQ-GFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQAN 682
            +P F TL+++ +    T G ++  G K   + ++ W TY  F +MV   +  L + G+G GD+VA +S NR+EWAVG  A+ +LG  +VPMYE Q++ E ++I+ DSGAK++   + E   +               Q     + N++     S      T D D  A + R      AE      S   +P D+A LIYTSGTTG PKGV L+H+N+  N+  M EV  + PED     SLAFLPWAH  G   EL  ++S GA +A+A+ +  I  ++ EV+P +LF+VP ++ ++YD +Q ++  +   +R++  + +AVA +R  +  +GK   L L  Q+   D +V SK+R   GGR++ +  GGS +S  V  F +  LGI + EGYGL+ETSP+ + + P  + R++GSVG+A+ G  + +  D     E  +GEI   G  V  GY  K EE    F    G   FRTGD+G L++ G+L ITGR+KE YKLENGKYV    +E++L  S +I   +++G + P+N  +IV + + L  W   K   GL  SS   E+   PEVQ   RE +    +D +K YE PQ++LL  E FTV N+MLTPKMS+KR  V+  Y  A+ A+Y + +
Sbjct:    3 LPQFQTLIDIFKRSTSTFGSRELFGEKK--NGQWVWTTYSRFGEMVDDLRGGLAQLGVGAGDRVAVISNNRLEWAVGAYATYTLGGAYVPMYESQQVKELQFIINDSGAKVVFCATDEIAQRL--------------QAVRAELPNVEHLIRFSGT----TSDADSFATLLR----RGAETPTPLVSP--KPTDLAGLIYTSGTTGQPKGVMLSHQNIARNVSAMHEVFPMTPEDR----SLAFLPWAHVFGQTVELHALLSMGASMAVAEAVEKIIDNLSEVKPTLLFSVPRIFNRIYDGLQKRMAGEKAVTRFMFHRGLAVAAQRRALAEQGKSSGL-LDLQHAFFDKVVFSKVRARFGGRLKYAFSGGSAISKEVAEFID-NLGITVYEGYGLTETSPIATANFP--ENRKIGSVGKALPGVRIEI--DTAATGEATQGEIVVHGHNVMMGYYNKPEENEKVF---TGNGGFRTGDMGLLDKDGYLYITGRIKEQYKLENGKYVVPSPIEQSLTLSTFIANALVHGMNKPYNVVVIVVDVDTLKKWATEK---GLDTSSLP-ELLKRPEVQQLYREQVNEFTRD-VKGYERPQRFLLTSEDFTVANDMLTPKMSVKRRNVVARYNDAIEALYREGS 597          

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|503705035|ref|WP_013939111.1| (long-chain fatty acid--CoA ligase [Myxococcus fulvus] >gi|337259812|gb|AEI65972.1| putative long-chain-fatty-acid--CoA ligase [Myxococcus fulvus HW-1])

HSP 1 Score: 402.134 bits (1032), Expect = 3.673e-128
Identity = 243/636 (38.21%), Postives = 358/636 (56.29%), Query Frame = 0

Query:   47 IPSFTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEVVRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLE-QGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQA 681
            +P F TL+++ +    T G ++  G K   + ++ W TY  F +MV   +  L + G+G GD+VA +S NR+EWAVG  A+ SLG  +VPMYE Q+  E ++I+ DSG+K++   + E   + +            A+   L  I ++ S T S        D D  A + R      AE      S   +P D+A LIYTSGTTG PKGV L+H N+  N+  M EV  P    +  SLAFLPWAH  G   EL  + S GA +A+A+ +  I  ++ EV+P +LF+VP ++ ++YD +Q ++  +   +R++  + +AVA ++  +  +GK   L L  Q+   D +V SK+R   GGR++ +  GGS +S  V  F +  LGI + EGYGL+ETSP+ + + P  + R++GSVG+A+ G  + +  D     E ++GEI   G  V  GY  K EE    F    G   FRTGD+G L+  G+L ITGR+KE YKLENGKYV    +E++L  S YI   +++G + P+N A+IV + + L  W   K   GL  SS   E+   PEV    RE +    +D +K YE PQ++LL  E FTV N+MLTPKMS+KR  V+  Y  A+ A+Y + 
Sbjct:    3 LPQFQTLIDILKRSTSTFGSRELFGEKK--NGQWVWTTYSRFGEMVDDLRGGLAQLGVGAGDRVAVISNNRLEWAVGAYATYSLGGAYVPMYESQQAKELQFIINDSGSKVVFCATDEIAQRIQSVR---------AELPHLEHI-IRFSGTTS--------DTDSFATLLR----RGAETPTPMVSP--KPTDLAGLIYTSGTTGQPKGVMLSHANIARNVSAMHEVF-PMGTDDR-SLAFLPWAHVFGQTVELHALFSMGASMAIAEAVEKIIDNLSEVKPTLLFSVPRIFNRIYDGLQKRMAGEKPVTRFMFRRGLAVAAEKRALAEQGKSSGL-LDLQHAFFDKVVFSKVRARFGGRLKYAFSGGSAISKEVAEFID-NLGITVYEGYGLTETSPIATANFP--ENRKIGSVGKALPGVRIEI--DTAATGEAKQGEIVVHGHNVMMGYYNKPEENEKVF---TGNGGFRTGDMGYLDPDGYLYITGRIKEQYKLENGKYVVPSPIEQSLALSTYIANALVHGMNKPYNVAIIVVDVDALKKWATEK---GLDTSSMP-ELLKRPEVLQLYREQVNEFTRD-VKGYERPQRFLLTSEDFTVANDMLTPKMSVKRRNVVARYNDAIEALYREG 596          

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|836728841|ref|WP_047860904.1| (long-chain fatty acid--CoA ligase [Archangium gephyra] >gi|827428142|gb|AKJ08038.1| Long-chain-fatty-acid--CoA ligase [Archangium gephyra])

HSP 1 Score: 399.438 bits (1025), Expect = 4.269e-127
Identity = 247/640 (38.59%), Postives = 354/640 (55.31%), Query Frame = 0

Query:   46 TIPSFTTLVEM-QQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEV--VRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTL-EQGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIYDQA 681
            T P F TLV++  QS+A + G +   G K   + ++ W+TY  F  MV   +  L + G+  GD+VA +S NR EWAVG  A  +LGA +VPMYE Q   E  YIL D GAK++                       FA  DA++         +  L+ I  F             +M   AD        +P D+A LIYTSGTTG PKGV L+H NL  N+  M EV  +  ED     SLAFLPWAHS G N EL  ++S GA + +A+ +  I  ++ EV+P +LF+VP ++ ++YD +Q ++ ++    R + ++ + VA++R + L E  + SL L AQ+   D +V SK+R   GGR++ +  G + +S  V  F +  LGI + EGYGL+ETSP+ + + P    R++GSVG+ + G  + + R     P  ++GEI   G  V  GY    +E +  F    G   F+TGD+G L + G++ ITGR+KE YKLENGKYV    +E+AL  S +I  V+L+G + PFN A+IVP+ + L  W   K   GL  SS   E+   PEVQ   RE I   CKD  K YE PQ++LL+ E FT  N+MLTP + +KR  V+K +G A+  +Y +A
Sbjct:    2 TSPKFETLVDIFLQSIA-SFGPRPLFGEKK--NGQWNWMTYIQFGQMVDDLRGGLAQLGVTQGDRVAVISNNRHEWAVGAYACYTLGAAYVPMYEAQLDKEWAYILNDCGAKVV-----------------------FAATDAIAAKLKAIKPELPKLEHIIRF-TGTATEADTFACLMRRGADTPSPVTTPKPSDLAGLIYTSGTTGNPKGVMLSHSNLARNVCAMHEVFPMSKEDR----SLAFLPWAHSFGQNVELNALISMGASMGIAEGVDKIVDNLSEVQPTLLFSVPRIFNRIYDGLQKRMAAEKPIKRMMFNRGIEVAKQR-KALAEKNQSSLLLDAQHAFFDRVVFSKVRARFGGRLKYAFSGAAAISREVAEFID-NLGITVYEGYGLTETSPIATANYPG--HRKIGSVGRPIPGTRVEIDRSETGDP--KQGEIVVYGHNVMMGYYNLPDENSKVFTADGG---FKTGDMGMLDDNGYVWITGRIKEQYKLENGKYVVPVPIEQALQLSPFITNVMLHGQNKPFNVAIIVPDMDSLKKWAAEK---GLDTSS-IPELLKRPEVQQLYREQINEFCKDAAKGYEKPQRFLLISEDFTTANDMLTPSLKLKRRNVLKKFGEAVEELYREA 597          

BLAST of EWM21593.1 vs. NCBI_GenBank
Match: gi|1101193841|gb|OIP44922.1| (long-chain fatty acid--CoA ligase [Deltaproteobacteria bacterium CG2_30_63_29])

HSP 1 Score: 396.741 bits (1018), Expect = 3.059e-126
Identity = 239/630 (37.94%), Postives = 357/630 (56.67%), Query Frame = 0

Query:   50 FTTLVEMQQSVARTQGKKDFLGYKPCPSSKYKWITYQAFNDMVGKAKFVLREAGIGPGDKVAALSGNRVEWAVGCMASLSLGATWVPMYEQQRLTECEYILRDSGAKLLLVGSREAYDKTRHFLTRPSRNPAFAQGDALSVINMQESKTVSSLKDIWTFDVDVPARMARVTGVMTAEADACFASAVVRPDDVAVLIYTSGTTGTPKGVELTHRNLCTNIEGMWEVVRPEDMPNALSLAFLPWAHSIGMNCELFNMMSRGARVALAQDMTTIASDIKEVRPNILFAVPTLYKKVYDRIQDKITSQGKWSRWLVDKAVAVAEKRLEILREGKRLSLGLQAQYEVLDHIVLSKLRDTLGGRVRISPVGGSKMSLPVTRFFEAALGIAILEGYGLSETSPLLSISRPDIQIRRLGSVGQAVKGAELAVVRDGLRVPEGEEGEIWASGPMVFKGYLKKKEETAAAFGEMDGKKWFRTGDLGTLE-QGFLTITGRLKELYKLENGKYVCAPVVEKALLESEYIFQVVLYGADLPFNTALIVPNWEKLTAWCVSKKLPGLSASSRKTEIRTHPEVQAFMRENIVLACKDKMKKYEMPQKWLLLEEPFTVENEMLTPKMSIKRHAVIKAYGSALNAIY 678
            F  LV+M +            G K     +YKWITY+     V   +  L   G+G GD VA ++ N   WA G  A+  L A +VPMYE Q   + +YI+ DSGAK+L+V +    +K + F+                    +E +T+  + +I   + D  +    +   +    D  +       DD+  LIYTSGTTG PKGV L+H NL +N+  + EV  P D  +  SL+FLPWAHS G   EL  ++S GA + LA+ + TI  ++ EV+P++LF+VP ++ ++YD +  K+ ++G   + L ++ + VA KR  + ++ K  +L L  Q++VLD +V  K+RD  GGR+R +  GG+ +S  V  F +  LGI + EGYGL+ETSP+ +++ PD Q  R+GS+G+A+ G E+ ++      PEG +GEI   GP V KGY    +ET   F E  G   F+TGD+G ++  GF+ ITGR+KE YKLENGKYV    +E+ L  S YI  ++++G + P+N A+IVP+ E L +W   K   GLS  + +  I+    V+A + E I    KD  K YE P+K++L  E FT EN+MLTP M IKR  V++ Y +ALN ++
Sbjct:    5 FQNLVDMFEKSVERYAANPLFGVKE--GGEYKWITYKDVGQQVKGLRGGLSALGVGKGDTVAIIANNSPAWAAGAYATYGLAARYVPMYEAQLAKDWKYIINDSGAKVLIVANDLIAEKCKDFV--------------------EELETLEFIVNIAGPESDAHSFAGLIKTGLATPVDPIYP----EKDDICGLIYTSGTTGMPKGVLLSHWNLTSNVNAVHEVF-PMDQTDR-SLSFLPWAHSFGQTVELHTLLSYGASMGLAESVQTIIQNLTEVKPSLLFSVPRIFNRIYDGVHKKMAAEGGLKKTLFERTLTVANKRRALAQDNKSSAL-LDLQFKVLDKLVGQKIRDRFGGRLRYAFSGGAALSKEVAIFID-NLGITVYEGYGLTETSPICTVNCPDGQ--RIGSIGRAIPGVEIKII-PFESAPEG-QGEIVVYGPNVMKGYHNLPKETEETFTEDGG---FKTGDMGHVDADGFVWITGRVKEQYKLENGKYVVPAPLEEKLKLSGYIANIMIHGVNKPYNVAVIVPDVEMLASWAKDK---GLSYDNDEALIKIDG-VEALINEEIQKYGKD-FKGYERPRKFVLTAEDFTTENDMLTPSMKIKRRKVLETYEAALNGLF 592