EWM22150.1, cds8527 (CDS) Nannochloropsis gaditana

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|585102571|gb|EWM22150.1| (cysteine desulfurase [Nannochloropsis gaditana])

HSP 1 Score: 3226.03 bits (8363), Expect = 0.000e+0
Identity = 1565/1565 (100.00%), Postives = 1565/1565 (100.00%), Query Frame = 0

Query:    1 MMEVEEEVASPSLPPASQPMLPSAHIQEHNHPGRNAKIGPVNFLQGQPSTGQGQREGRPAGSEAIGEIHSCKMEKRRRQGDGDRECSYRTFYELLEEKEALGELLMQRTLKNGGNPPDIVREIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLNNWRQLQQQHRQQQEKKQHQKKAQQQPQKEREPPHQLHLPSPRPSPRTSQPILEERGGPEPAQYEEGKRMVEKKVNGSSRPHHPAQPSRPQEPHQRMPGCGDSGRPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLGDFQSPRLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLASCTYGMGRARKGGHVGLAHTSGGEQEHKEQKEKEGKERVPNVDGDDKEGMDVMEDWEEKTRCSRKTQEKVSAAVAEWGCESEVELFGMLMEEAEALTEALPDPPAPSFPSALRPEHEHLRFFVWPWEVGMEGVGSKGSGDRAKEEASKSAVEEGEQGRTLQKEEERKTRTIEKGGASQENDALRLPTLSFGPITSDPCVPIPPSRPPGPAPTYGPGYFRPLQVTGVRESPRYQYRELLFENLTEHTRSSSRSSLSLSGSGSSLASWNSTAAGSVASSPGICPSPSPSWRMRALSLGNDSPSLSEWKSQSQLLVERTFLPSFSYTSSVSPNHPQVQYRTGPGGEGRRAGSRDSTPSRKRSHGGNEKGPSAGEDRGRCTPTSPSLLTRRPSHGQLSMLAENLKRIATGGHHLPQQPPRSVLHPDKGQQKQHLNSPYHQSAPVAPPCTTASDATCQGEQPGAVPQDLVRGHDWSKSDHGGSSQDGQRGGATTTHMASNDGASTPIPFQCRQRTPSMRPTVPSSCPQMHHLRHQHPHAQQPHAQKQQSEQPQHQQQPHQQQQQQQQQQGNPPNPSIHSAVKGSGSSSAPLPGHPRVFCPVGAASAFSACAPSGSLMSSSSTTTATSTSSSLLSSPPPPPPPPPHRTEKLTFYTHAGGVLGPQAIQLVQSSFEVIKPAGDAFCTCFYDRLFSQHPELETHFRYTDMKHQHASLLSALVLVIQELLDPSSLRSRLLQLGKRHFYYGASPAHYGHVGAAMLWALSTHLGERMTQEIHDAWSDTITFIATVMIEGGKLEEEAVNLAQEESEREIRRLKEEAEMAQTMVKESHATT 1565
            MMEVEEEVASPSLPPASQPMLPSAHIQEHNHPGRNAKIGPVNFLQGQPSTGQGQREGRPAGSEAIGEIHSCKMEKRRRQGDGDRECSYRTFYELLEEKEALGELLMQRTLKNGGNPPDIVREIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLNNWRQLQQQHRQQQEKKQHQKKAQQQPQKEREPPHQLHLPSPRPSPRTSQPILEERGGPEPAQYEEGKRMVEKKVNGSSRPHHPAQPSRPQEPHQRMPGCGDSGRPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLGDFQSPRLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLASCTYGMGRARKGGHVGLAHTSGGEQEHKEQKEKEGKERVPNVDGDDKEGMDVMEDWEEKTRCSRKTQEKVSAAVAEWGCESEVELFGMLMEEAEALTEALPDPPAPSFPSALRPEHEHLRFFVWPWEVGMEGVGSKGSGDRAKEEASKSAVEEGEQGRTLQKEEERKTRTIEKGGASQENDALRLPTLSFGPITSDPCVPIPPSRPPGPAPTYGPGYFRPLQVTGVRESPRYQYRELLFENLTEHTRSSSRSSLSLSGSGSSLASWNSTAAGSVASSPGICPSPSPSWRMRALSLGNDSPSLSEWKSQSQLLVERTFLPSFSYTSSVSPNHPQVQYRTGPGGEGRRAGSRDSTPSRKRSHGGNEKGPSAGEDRGRCTPTSPSLLTRRPSHGQLSMLAENLKRIATGGHHLPQQPPRSVLHPDKGQQKQHLNSPYHQSAPVAPPCTTASDATCQGEQPGAVPQDLVRGHDWSKSDHGGSSQDGQRGGATTTHMASNDGASTPIPFQCRQRTPSMRPTVPSSCPQMHHLRHQHPHAQQPHAQKQQSEQPQHQQQPHQQQQQQQQQQGNPPNPSIHSAVKGSGSSSAPLPGHPRVFCPVGAASAFSACAPSGSLMSSSSTTTATSTSSSLLSSPPPPPPPPPHRTEKLTFYTHAGGVLGPQAIQLVQSSFEVIKPAGDAFCTCFYDRLFSQHPELETHFRYTDMKHQHASLLSALVLVIQELLDPSSLRSRLLQLGKRHFYYGASPAHYGHVGAAMLWALSTHLGERMTQEIHDAWSDTITFIATVMIEGGKLEEEAVNLAQEESEREIRRLKEEAEMAQTMVKESHATT
Sbjct:    1 MMEVEEEVASPSLPPASQPMLPSAHIQEHNHPGRNAKIGPVNFLQGQPSTGQGQREGRPAGSEAIGEIHSCKMEKRRRQGDGDRECSYRTFYELLEEKEALGELLMQRTLKNGGNPPDIVREIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLNNWRQLQQQHRQQQEKKQHQKKAQQQPQKEREPPHQLHLPSPRPSPRTSQPILEERGGPEPAQYEEGKRMVEKKVNGSSRPHHPAQPSRPQEPHQRMPGCGDSGRPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLGDFQSPRLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLASCTYGMGRARKGGHVGLAHTSGGEQEHKEQKEKEGKERVPNVDGDDKEGMDVMEDWEEKTRCSRKTQEKVSAAVAEWGCESEVELFGMLMEEAEALTEALPDPPAPSFPSALRPEHEHLRFFVWPWEVGMEGVGSKGSGDRAKEEASKSAVEEGEQGRTLQKEEERKTRTIEKGGASQENDALRLPTLSFGPITSDPCVPIPPSRPPGPAPTYGPGYFRPLQVTGVRESPRYQYRELLFENLTEHTRSSSRSSLSLSGSGSSLASWNSTAAGSVASSPGICPSPSPSWRMRALSLGNDSPSLSEWKSQSQLLVERTFLPSFSYTSSVSPNHPQVQYRTGPGGEGRRAGSRDSTPSRKRSHGGNEKGPSAGEDRGRCTPTSPSLLTRRPSHGQLSMLAENLKRIATGGHHLPQQPPRSVLHPDKGQQKQHLNSPYHQSAPVAPPCTTASDATCQGEQPGAVPQDLVRGHDWSKSDHGGSSQDGQRGGATTTHMASNDGASTPIPFQCRQRTPSMRPTVPSSCPQMHHLRHQHPHAQQPHAQKQQSEQPQHQQQPHQQQQQQQQQQGNPPNPSIHSAVKGSGSSSAPLPGHPRVFCPVGAASAFSACAPSGSLMSSSSTTTATSTSSSLLSSPPPPPPPPPHRTEKLTFYTHAGGVLGPQAIQLVQSSFEVIKPAGDAFCTCFYDRLFSQHPELETHFRYTDMKHQHASLLSALVLVIQELLDPSSLRSRLLQLGKRHFYYGASPAHYGHVGAAMLWALSTHLGERMTQEIHDAWSDTITFIATVMIEGGKLEEEAVNLAQEESEREIRRLKEEAEMAQTMVKESHATT 1565          

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|698787984|ref|XP_009827024.1| (hypothetical protein H257_04291 [Aphanomyces astaci] >gi|574118113|gb|ETV83594.1| hypothetical protein H257_04291 [Aphanomyces astaci])

HSP 1 Score: 429.483 bits (1103), Expect = 2.728e-126
Identity = 208/400 (52.00%), Postives = 265/400 (66.25%), Query Frame = 0

Query:  343 RPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLGDFQSPRLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLASCTY 742
            +PV+F  PF HHSN+LPWRE+   +V +       +D+  L+L L  Y  R LKIG+F+AASN+TG+  DV  IT LLH++GALAFWDY+ AAPY+ MDMN                                 P +  + RP    DA++LSGHK VGGPG+PGVLVVKK L+ N VP  PGGGTVF+V +  HRYLSN+ EREEGGTPD LGSIRLGL F +K+R+G     +R     ++ + +  ++R++V+LG   + RLPIFSFL+R G  FLHYNFVCALLNDLFG+QSRGGCQCAGPYGQRLLG+S       E AL++  EVLRPG SR+S  Y   D EV+YI++A+ FVA  G KFLP YR N +TGEW++ T+F   P R+WL+  T+
Sbjct:  178 KPVVFVGPFEHHSNLLPWRESSADVVQIGESSDGTVDIAALKLQLTTYADRPLKIGSFSAASNLTGVLSDVDGITCLLHQHGALAFWDYAAAAPYVAMDMN---------------------------------PVVVGDTRPFVYKDAIFLSGHKFVGGPGSPGVLVVKKRLLGNAVPTTPGGGTVFYVTEDDHRYLSNREEREEGGTPDTLGSIRLGLAFEIKQRIGTATILDREHRHVQRVVAALQNHRHLVLLGHGTADRLPIFSFLVRAGARFLHYNFVCALLNDLFGVQSRGGCQCAGPYGQRLLGISKRDQRTLESALIDKTEVLRPGMSRVSFPYIMDDDEVEYILAALDFVATEGWKFLPQYRFNHKTGEWKHRTRFTKFPTRKWLSKATF 544          

HSP 2 Score: 127.487 bits (319), Expect = 7.645e-26
Identity = 64/121 (52.89%), Postives = 87/121 (71.90%), Query Frame = 0

Query:  119 IVREIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLN 239
            ++  I+ +++GR   F +P G  RP+VYAD+TASGR+L+ IED+M  EV+P YGNTHTTTS+TGLQ+T +R E R ++A  +NA I G G     AEDV+ F GSGSTGA+ KL+  L L+
Sbjct:   55 LLSHIRSTVIGRNSAFDTPFG-VRPLVYADYTASGRTLQCIEDYMQTEVLPQYGNTHTTTSITGLQSTCFRHETRQIVAQCVNAKITGRG-----AEDVVLFTGSGSTGAIHKLVLALGLH 169          

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|298708709|emb|CBJ49206.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 434.491 bits (1116), Expect = 1.052e-125
Identity = 223/413 (54.00%), Postives = 269/413 (65.13%), Query Frame = 0

Query:  336 PGCGDSGRPVIFSCPFGHHSNMLPWRETGCKIVNVKAD-GRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLGDFQSPRLPIFSFLIRHGPS---FLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLASCTYGM 744
            PG     RPVIF  P  HHSN+LPWRE+  ++V V+ +    G+D+ HLE  LR++  R LKIGAF+AASN+TG+  DV  +TA LHR GALA WDY+TAAPY+ +DMN                               P  S   + +     DA++ SGHK VGGPGTPGVLVVKK L+ N VP   GGGTVFFV +  HRYLSN+ EREEGGTPDILGSIRLGL   +KE +G      R    A+  L +  +++ IVVLGD    RLPIFSFL+R   S   FLHYNFVCALLNDLFG+QSRGGCQCAGPY Q LLGLS   + A E  LL   E+LRPGFSRLSL++ +SD E  YI+ AV FVADHG+ FLP+YR N +TGEW++ T+F   P RRWL +C YG 
Sbjct:  202 PGWNPDDRPVIFVGPLEHHSNLLPWRESCGEVVTVRENPSSGGVDLAHLEEQLRRFASRRLKIGAFSAASNVTGLLEDVDKVTATLHRAGALALWDYATAAPYVHVDMN-------------------------------PSASGLEDDQALVAKDAIFFSGHKFVGGPGTPGVLVVKKRLLCNAVPGTVGGGTVFFVTEKDHRYLSNKEEREEGGTPDILGSIRLGLAVQMKESMGRRGLQGREEALAELGLSTLRAHKGIVVLGDSGKKRLPIFSFLVRTASSSGRFLHYNFVCALLNDLFGVQSRGGCQCAGPYSQLLLGLSLPDTRAVEAELLRKHELLRPGFSRLSLSFVNSDREAKYILDAVLFVADHGASFLPYYRVNYKTGEWKHKTRFTRFPERRWLGNCDYGF 583          

HSP 2 Score: 119.398 bits (298), Expect = 3.201e-23
Identity = 56/116 (48.28%), Postives = 80/116 (68.97%), Query Frame = 0

Query:  123 IQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRL 238
            ++  LLGR  PF +P G  +P+VY+DWTA+GR++  IE ++ + V+P +GNTHT TS+TG QT+ +R EAR +IA A+NA + G       AEDV+ F G+G+T AV KL+  L L
Sbjct:   87 VRRGLLGRNTPFRTPYG-MKPLVYSDWTATGRAVASIETYLADRVVPLFGNTHTATSITGAQTSCFRHEARQIIAQAVNAKVTGRA-----AEDVVLFTGTGATAAVAKLVSALGL 196          

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|673029960|ref|XP_008868941.1| (hypothetical protein H310_05873 [Aphanomyces invadans] >gi|574475336|gb|ETW02336.1| hypothetical protein H310_05873 [Aphanomyces invadans])

HSP 1 Score: 424.861 bits (1091), Expect = 1.188e-124
Identity = 207/400 (51.75%), Postives = 266/400 (66.50%), Query Frame = 0

Query:  343 RPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLGDFQSPRLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLASCTY 742
            +PV+F  PF HHSN+LPWRE+   +V +       +D+  L+  L KY  R LKIG+F+AASN+TG+  DV AI  LLH++GAL+FWDY+ AAPY+ +DMN                                 P +  ++RP    DA++LSGHK +GGPG+PGVLVVKK L+ N VP  PGGGTVF+V +  HRYLSN+ EREEGGTPD LGSIRLGL F +K+R+G+     R     ++ + +  ++ ++V+LG   + RLPIFSFL+R G  FLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLG+S     A E AL++  EVLRPG SR+S  Y   D EVDYI++A+ FVA  G KFLP YR N +TGEW++ T+F   P R+WL+  T+
Sbjct:  181 KPVVFVGPFEHHSNLLPWRESSADVVQISETSEGIVDVSMLKRELIKYADRPLKIGSFSAASNLTGVLTDVDAIACLLHQHGALSFWDYAAAAPYVPIDMN---------------------------------PVVAGDSRPFVYKDAIFLSGHKFLGGPGSPGVLVVKKRLLGNAVPTAPGGGTVFYVTEEDHRYLSNREEREEGGTPDTLGSIRLGLAFEIKQRIGSQTILAREHRNVQRVVTALQNHSHLVLLGHSTADRLPIFSFLVRSGARFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGISKSHQRALEAALIDKTEVLRPGMSRVSFPYIMDDAEVDYILAALDFVATDGWKFLPQYRFNHKTGEWKHRTRFTKFPTRKWLSKATF 547          

HSP 2 Score: 131.724 bits (330), Expect = 3.913e-27
Identity = 67/121 (55.37%), Postives = 86/121 (71.07%), Query Frame = 0

Query:  119 IVREIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLN 239
            + R I+ +++G    F +P G  RP+VYAD+TASGRSL+ IED+M  EV+P YGNTHTTTS+TGLQTT +R E R +IA  +NA I G G     AEDV+ F GSGSTGA+ KL+  L L+
Sbjct:   58 LFRHIRSTVIGHNSAFDTPFG-TRPLVYADYTASGRSLQCIEDYMQTEVLPQYGNTHTTTSITGLQTTCFRHETRQIIAQCVNAKITGRG-----AEDVVLFTGSGSTGAIAKLVQALGLH 172          

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|1173945389|gb|OQR94019.1| (cysteine desulfurase [Thraustotheca clavata])

HSP 1 Score: 423.32 bits (1087), Expect = 3.639e-124
Identity = 212/407 (52.09%), Postives = 265/407 (65.11%), Query Frame = 0

Query:  343 RPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLG---DFQSPR-LPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLASCTYGMG 745
            RPV+F  PF HHSN+LPWRE+  +IV +  D    +D++ L+  L +Y    LKIG+F+AASN+TG    V AIT LLH +GALAFWDY+  APY+ MDMN                                 P L  + RP    D ++ SGHK +GGPG+PGVLV+KK L+ N VP  PGGGTVF+V +  HRYLSN+ EREEGGTPD LGSIRLGL F +K R+G  Y  ER      K L++  ++ ++V+LG   D  +PR LPIFSFLI+ G  FLHYNFVCALLNDLFG+QSRGGCQCAGPYGQRLLG+S     A E AL++ +EVLRPGFSR+S  Y   D +VDY + A+ FVA HG KFLP YR N +TGEW++ T+F   P R+WL+   +  G
Sbjct:  156 RPVVFVGPFEHHSNLLPWRESNTEIVQINEDANGHVDVKMLKAKLIEYASHPLKIGSFSAASNLTGQLSYVDAITCLLHVHGALAFWDYAACAPYIAMDMN---------------------------------PVLTGDMRPFAYKDGIFFSGHKFLGGPGSPGVLVLKKRLLGNAVPTNPGGGTVFYVTEDGHRYLSNRAEREEGGTPDTLGSIRLGLAFEVKHRIGTKYIIEREEANVVKVLKALENHSHVVLLGHHSDSVAPRRLPIFSFLIKCGDRFLHYNFVCALLNDLFGVQSRGGCQCAGPYGQRLLGISKQDQLAIEDALIDKIEVLRPGFSRVSFPYIMDDKDVDYTLKAIDFVALHGWKFLPQYRFNHKTGEWKHKTRFTKFPTRKWLSRAPFLSG 529          

HSP 2 Score: 132.494 bits (332), Expect = 2.580e-27
Identity = 73/151 (48.34%), Postives = 99/151 (65.56%), Query Frame = 0

Query:   89 RTFYELLEEKEALGELLMQRTLKNGGNPPDIV-REIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRL 238
            RTF + LEE     +L +     NG +   ++ + +Q SL+G    F +P G R P++YAD+TASGRSL+ IED+M  EV+P YGNTHTTTS+TGLQ+T +R E R ++A  +NA I G G     AEDV+ F GSGST A+ KL+ +L L
Sbjct:    6 RTFCDELEE-----DLNVLDQENNGVDSSKVLLKTMQSSLVGNHVAFDTPFGPR-PLIYADFTASGRSLQCIEDYMQSEVLPLYGNTHTTTSITGLQSTCFRHETRQIVAQCVNAKITGRG-----AEDVVLFTGSGSTSAINKLVQILGL 145          

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|970634017|gb|KUF79431.1| (tRNA 2-thiocytidine biosynthesis protein TtcA [Phytophthora nicotianae])

HSP 1 Score: 422.165 bits (1084), Expect = 4.583e-124
Identity = 205/398 (51.51%), Postives = 271/398 (68.09%), Query Frame = 0

Query:  343 RPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLG--DFQSPRLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLA 738
            +PV+F  PF HHSN+LPWRE+  +IV +  +    +D++ LE  L+ Y  R LKIGAF+AASN+TG+  DV A++ALLH++GALA WDY+T APY+ +DMN                        P  + +  RP +       YK DA+++SGHK +GGPG+PGVL+VKK L+ N VP  PGGGTVFFV ++ HRYLSN+ EREEGGTPDILGSIRLGL F LK+RVG            ++  ES   N+NIV+LG  D    ++P+FSFL+R G  FLHYNFVCALLNDLFG+Q+RGGCQCAGPY  RLLGL+     A E AL++  E+LRPGF+R+S  Y+ SD E++Y++SAV+FVA  G KFLP Y+ N ++G W++ T+F   P R+W++
Sbjct:   78 KPVVFVGPFEHHSNLLPWRESAAEIVTIPENEHGQMDLKALETQLQMYAARPLKIGAFSAASNLTGVLADVDAVSALLHQHGALACWDYATCAPYVNIDMN------------------------PVVTDETRRPFV-------YK-DAVFISGHKFIGGPGSPGVLIVKKRLLTNAVPTVPGGGTVFFVTENDHRYLSNRAEREEGGTPDILGSIRLGLAFELKQRVGTKNIMILERRNVQRVRESLQQNKNIVLLGRQDSAVEQIPVFSFLVRFGDRFLHYNFVCALLNDLFGVQTRGGCQCAGPYATRLLGLARRDVLALESALIDKKEMLRPGFTRMSFPYFMSDAELEYVLSAVNFVATDGWKFLPQYKFNHKSGVWKHWTRFTKFPDRKWIS 443          

HSP 2 Score: 85.5001 bits (210), Expect = 5.908e-13
Identity = 41/74 (55.41%), Postives = 50/74 (67.57%), Query Frame = 0

Query:  168 MPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLNNW 241
            MP YGNTHTTTS+TGLQTT +R EAR ++A A+NA I G G     AED + F G GST A+ KL+  L L  +
Sbjct:    1 MPLYGNTHTTTSITGLQTTCFRHEARQIVAQAVNAKITGRG-----AEDCVLFTGQGSTSAINKLVQALGLQQF 69          

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|1173954103|gb|OQS01596.1| (hypothetical protein ACHHYP_00577 [Achlya hypogyna])

HSP 1 Score: 422.165 bits (1084), Expect = 8.087e-124
Identity = 208/413 (50.36%), Postives = 268/413 (64.89%), Query Frame = 0

Query:  335 MPGCGDSGRPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLG-DFQSP-RLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLASCTYGMG 745
            +PG G   RPV+F  PF HHSN+LPWRE+  ++V +  +    +DM  L   L +Y  R LKIG+F+AASN+TG   DV A+T LLH++GAL+FWDY+  APY+ MDMN                                 P L  + RP    D ++ SGHK VGGPG+PGVLV+KK L+ N VP  PGGGTVF+V +  HRYLSN+ EREEGGTPD LG+IRLGL   +K R+G +Y  ER      + L +  ++ ++V+LG D  +P RLPIFSFL+R G  FLHYNFVCALLNDLFG+QSRGGCQCAGPYGQRLLG++     A E AL++  EVLRPGFSR+S  Y   + +V +++ A+ FVA HG KFLP YR N +TGEW++ T+F   P R+WL+   +  G
Sbjct:  149 LPGVG---RPVVFVGPFEHHSNLLPWRESAAEVVQIAENAEGNVDMVMLAAKLVEYADRPLKIGSFSAASNLTGQLTDVDAVTVLLHQHGALSFWDYAACAPYVSMDMN---------------------------------PVLTDDRRPFAYKDGIFFSGHKFVGGPGSPGVLVLKKRLLGNSVPSCPGGGTVFYVTEDDHRYLSNRAEREEGGTPDNLGAIRLGLALEVKHRIGVSYILEREEAHVAQVLAALSAHPSVVLLGHDVAAPTRLPIFSFLVRAGDRFLHYNFVCALLNDLFGVQSRGGCQCAGPYGQRLLGVTKADQRALEAALIDKTEVLRPGFSRVSFPYIMDNSDVAFVLRAIEFVATHGWKFLPQYRFNHKTGEWKHKTRFTKFPTRKWLSKAAFLSG 525          

HSP 2 Score: 131.724 bits (330), Expect = 4.264e-27
Identity = 66/121 (54.55%), Postives = 86/121 (71.07%), Query Frame = 0

Query:  119 IVREIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLN 239
             +R ++ SL+G   PF +P G R P+VYAD+TASGRSL  IED+M  EV+P YGNTHTTTS+TGLQ+T +R E R ++A  +NA I G G     AEDV+ F GSGST A+ KL+ +L L+
Sbjct:   31 FLRSMRASLVGHKVPFDTPYGPR-PLVYADFTASGRSLACIEDYMRAEVLPLYGNTHTTTSITGLQSTCFRHETRQIVAQCVNAKITGRG-----AEDVVLFTGSGSTAAINKLVQILGLH 145          

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|1214682733|gb|OWZ00908.1| (hypothetical protein PHMEG_00027801 [Phytophthora megakarya])

HSP 1 Score: 423.705 bits (1088), Expect = 1.597e-123
Identity = 215/444 (48.42%), Postives = 284/444 (63.96%), Query Frame = 0

Query:  341 SGRPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLG--DFQSPRLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLASCTYGMGRARKGGHVGLAHTSGGEQEHKEQKEKEGKERVPNVDG 782
            S RPV+F  PF HHSN+LPWRE+G +IV +  + +  +D++ LE  L+    R LKIGAF+AASN+TG+  DV A++ALLH++GALA WDY+T APY+ +DMN                        P  + +  RP +       YK DA+++SGHK +GGPG+PGVLVVKK L+ N VP  PGGGTVFFV ++ HRYLSN+ EREEGGTPDILGSIRLGL F LK+RVG            ++  ES   N NIV+LG  D +  ++P+FSFL+R G  FLHYNFVCALLNDLFGIQ+RGGCQCAGPY  RLLGL+     A E AL+E  E+LRPGF+R+S  Y+ S  E++Y+++AV+FVA  G KFLP Y+ N ++G W++ T+F   P R+W++   +      K     LA        H+ +   E +  V  V+ 
Sbjct:  156 SERPVVFVGPFEHHSNLLPWRESGAEIVTIPENQQGQMDLQALETQLKANAARPLKIGAFSAASNLTGVLTDVDAVSALLHQHGALACWDYATCAPYVNIDMN------------------------PVVTDEQRRPFV-------YK-DAVFISGHKFIGGPGSPGVLVVKKRLLTNAVPTVPGGGTVFFVTENDHRYLSNRAEREEGGTPDILGSIRLGLAFELKQRVGTKNIMTLERRNVQRVRESLQQNENIVLLGRQDSEVEQIPVFSFLVRFGDRFLHYNFVCALLNDLFGIQTRGGCQCAGPYATRLLGLARRDVLALESALIEKKEMLRPGFTRMSFPYFMSTAELEYVLAAVNFVATDGWKFLPQYKFNHKSGVWKHWTRFTKFPDRKWISH--FATALEEKTRSKNLADDEETLAAHRAENLAEARRLVDEVNA 565          

HSP 2 Score: 125.176 bits (313), Expect = 4.381e-25
Identity = 74/160 (46.25%), Postives = 95/160 (59.38%), Query Frame = 0

Query:   83 DRECSYRTFYELLEEKEALGELLMQ-RTLKNGGNPPDIVREIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLNNW 241
            D   S R F E LEE  +L     Q  +L       D V  I  +++GR   F SP G R  + YAD+TAS + L+ +ED++  EVMP YGNTHTTTS+TGLQTT +R EAR ++A A+NA I G G     AED + F G GST AV+KL+  L L  +
Sbjct:    3 DESASSRYFCEELEEDLSLQIQESQPESLSTAAQ--DAVDLITRNVVGRNSLFESPFGSR-ALCYADFTASSKPLQCVEDYLNREVMPLYGNTHTTTSITGLQTTCFRHEARQIVAQAVNAKITGRG-----AEDCVLFTGQGSTSAVVKLVQALGLQQF 154          

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|567951032|gb|ETK76739.1| (hypothetical protein L915_16922 [Phytophthora parasitica] >gi|567979694|gb|ETL30170.1| hypothetical protein L916_16825 [Phytophthora parasitica] >gi|568009187|gb|ETL83396.1| hypothetical protein L917_16658 [Phytophthora parasitica] >gi|570941075|gb|ETP06332.1| hypothetical protein F441_17268 [Phytophthora parasitica CJ01A1])

HSP 1 Score: 423.32 bits (1087), Expect = 1.741e-123
Identity = 205/398 (51.51%), Postives = 271/398 (68.09%), Query Frame = 0

Query:  343 RPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLG--DFQSPRLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLA 738
            +PV+F  PF HHSN+LPWRE+  +IV +  +    +D++ LE  L+ Y  R LKIGAF+AASN+TG+  DV A++ALLH++GALA WDY+T APY+ +DMN                        P  + +  RP +       YK DA+++SGHK +GGPG+PGVL+VKK L+ N VP  PGGGTVFFV ++ HRYLSN+ EREEGGTPDILGSIRLGL F LK+RVG            ++  ES   N+NIV+LG  D    ++P+FSFL+R G  FLHYNFVCALLNDLFG+Q+RGGCQCAGPY  RLLGL+     A E AL++  E+LRPGF+R+S  Y+ SD E++Y++SAV+FVA  G KFLP Y+ N ++G W++ T+F   P R+W++
Sbjct:  171 KPVVFVGPFEHHSNLLPWRESAAEIVTIPENEHGQMDLKALETQLQMYAARPLKIGAFSAASNLTGVLADVDAVSALLHQHGALACWDYATCAPYVNIDMN------------------------PVVTDETRRPFV-------YK-DAVFISGHKFIGGPGSPGVLIVKKRLLTNAVPTVPGGGTVFFVTENDHRYLSNRAEREEGGTPDILGSIRLGLAFELKQRVGTKNIMILERRNVQRVRESLQQNKNIVLLGRQDSAVEQIPVFSFLVRFGDRFLHYNFVCALLNDLFGVQTRGGCQCAGPYATRLLGLARRDVLALESALIDKKEMLRPGFTRMSFPYFMSDAELEYVLSAVNFVATDGWKFLPQYKFNHKSGVWKHWTRFTKFPDRKWIS 536          

HSP 2 Score: 119.783 bits (299), Expect = 1.829e-23
Identity = 61/122 (50.00%), Postives = 80/122 (65.57%), Query Frame = 0

Query:  120 VREIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLNNW 241
            V  I  +++GR   F SP G R  + YAD+TAS + L+ +ED++  EVMP YGNTHTTTS+TGLQTT +R EAR ++A A+NA I G G     AED + F G GST A+ KL+  L L  +
Sbjct:   47 VDLITRNVVGRQTLFESPFGSR-VLCYADFTASSKPLQCVEDYLNREVMPLYGNTHTTTSITGLQTTCFRHEARQIVAQAVNAKITGRG-----AEDCVLFTGQGSTSAINKLVQALGLQQF 162          

BLAST of EWM22150.1 vs. NCBI_GenBank
Match: gi|570971319|gb|ETP34438.1| (hypothetical protein F442_17253 [Phytophthora parasitica P10297])

HSP 1 Score: 423.32 bits (1087), Expect = 1.758e-123
Identity = 205/398 (51.51%), Postives = 271/398 (68.09%), Query Frame = 0

Query:  343 RPVIFSCPFGHHSNMLPWRETGCKIVNVKADGRRGLDMRHLELMLRKYQHRFLKIGAFTAASNITGICLDVPAITALLHRYGALAFWDYSTAAPYLKMDMNASSWDGGAMLGSQGAARSEGEEIGPTGSPQGPRPSLPPNARPNYKIDALYLSGHKLVGGPGTPGVLVVKKFLVQNDVPERPGGGTVFFVKDHSHRYLSNQVEREEGGTPDILGSIRLGLCFGLKERVGATYATERGALFAKKTLESFCSNRNIVVLG--DFQSPRLPIFSFLIRHGPSFLHYNFVCALLNDLFGIQSRGGCQCAGPYGQRLLGLSPDASDAYEHALLEHLEVLRPGFSRLSLTYYHSDPEVDYIISAVHFVADHGSKFLPFYRCNERTGEWRYHTQFGSAPGRRWLA 738
            +PV+F  PF HHSN+LPWRE+  +IV +  +    +D++ LE  L+ Y  R LKIGAF+AASN+TG+  DV A++ALLH++GALA WDY+T APY+ +DMN                        P  + +  RP +       YK DA+++SGHK +GGPG+PGVL+VKK L+ N VP  PGGGTVFFV ++ HRYLSN+ EREEGGTPDILGSIRLGL F LK+RVG            ++  ES   N+NIV+LG  D    ++P+FSFL+R G  FLHYNFVCALLNDLFG+Q+RGGCQCAGPY  RLLGL+     A E AL++  E+LRPGF+R+S  Y+ SD E++Y++SAV+FVA  G KFLP Y+ N ++G W++ T+F   P R+W++
Sbjct:  171 KPVVFVGPFEHHSNLLPWRESAAEIVTIPENEHGQMDLKALETQLQMYAARPLKIGAFSAASNLTGVLADVDAVSALLHQHGALACWDYATCAPYVNIDMN------------------------PVVTDETRRPFV-------YK-DAVFISGHKFIGGPGSPGVLIVKKRLLTNAVPTVPGGGTVFFVTENDHRYLSNRAEREEGGTPDILGSIRLGLAFELKQRVGTKNIMILERRNVQRVRESLQQNKNIVLLGRQDSAVEQIPVFSFLVRFGDRFLHYNFVCALLNDLFGVQTRGGCQCAGPYATRLLGLARRDVLALESALIDKKEMLRPGFTRMSFPYFMSDAELEYVLSAVNFVATDGWKFLPQYKFNHKSGVWKHWTRFTKFPDRKWIS 536          

HSP 2 Score: 120.168 bits (300), Expect = 1.723e-23
Identity = 61/122 (50.00%), Postives = 80/122 (65.57%), Query Frame = 0

Query:  120 VREIQLSLLGRLRPFASPLGGRRPIVYADWTASGRSLRFIEDFMCEEVMPFYGNTHTTTSVTGLQTTLYRQEARHLIAAAINAHIDGEGGANEQAEDVLFFCGSGSTGAVMKLIHVLRLNNW 241
            V  I  +++GR   F SP G R  + YAD+TAS + L+ +ED++  EVMP YGNTHTTTS+TGLQTT +R EAR ++A A+NA I G G     AED + F G GST A+ KL+  L L  +
Sbjct:   47 VDLITRNVVGRQTLFESPFGSR-VLCYADFTASSKPLQCVEDYLNREVMPLYGNTHTTTSITGLQTTCFRHEARQIVAQAVNAKITGRG-----AEDCVLFTGQGSTSAINKLVQTLGLQQF 162