EWM29817.1, cds840 (CDS) Nannochloropsis gaditana

Overview
NameEWM29817.1
Unique Namecds840
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002456.1:544562..544685 -
Alignment locationCM002456.1:543164..544239 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM29817.1
Productmicrocystin synthetase-associated thioesterase
Orig transcript idgnl|cribi|Naga_100017g31.315.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002456.1supercontigCM002456.1:544562..544685 -
CM002456.1supercontigCM002456.1:543164..544239 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
GO:0016788hydrolase activity, acting on ester bonds
Vocabulary: Biological Process
TermDefinition
GO:0009058biosynthetic process
Vocabulary: INTERPRO
TermDefinition
IPR012223TEII
IPR001031Thioesterase
IPR029058AB_hydrolase
Homology
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|585112398|gb|EWM29817.1| (microcystin synthetase-associated thioesterase [Nannochloropsis gaditana])

HSP 1 Score: 829.321 bits (2141), Expect = 0.000e+0
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0
Query:    1 MLTPFALYSLKTTGAGTKGSLKWSVVVFSVVSYHAILVEVMGCTSSKGCCLCCVPDAALKSCGPAVPIAKEITNGYPAPPGHGTHTPTHSQGLGEAIQTRTRCISACAVRCIHDHLPGHRHDHQRAPAKMREEEARKWLSVKRKDFARVRLVCFPYAGGNSNFYKTWDVKEAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSVIFRDLTGPGATDLPGFILFDPNKK 399
            MLTPFALYSLKTTGAGTKGSLKWSVVVFSVVSYHAILVEVMGCTSSKGCCLCCVPDAALKSCGPAVPIAKEITNGYPAPPGHGTHTPTHSQGLGEAIQTRTRCISACAVRCIHDHLPGHRHDHQRAPAKMREEEARKWLSVKRKDFARVRLVCFPYAGGNSNFYKTWDVKEAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSVIFRDLTGPGATDLPGFILFDPNKK
Sbjct:    1 MLTPFALYSLKTTGAGTKGSLKWSVVVFSVVSYHAILVEVMGCTSSKGCCLCCVPDAALKSCGPAVPIAKEITNGYPAPPGHGTHTPTHSQGLGEAIQTRTRCISACAVRCIHDHLPGHRHDHQRAPAKMREEEARKWLSVKRKDFARVRLVCFPYAGGNSNFYKTWDVKEAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSVIFRDLTGPGATDLPGFILFDPNKK 399          
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|585100986|gb|EWM20990.1| (microcystin synthetase-associated thioesterase [Nannochloropsis gaditana])

HSP 1 Score: 238.424 bits (607), Expect = 3.077e-72
Identity = 138/340 (40.59%), Postives = 195/340 (57.35%), Query Frame = 0
Query:   41 MGCTSSKGCCLCCVPDAALKSCGPAVPIAKEITNGYPAPPGHGTHTPTHSQGLGEAIQTRTRCISACAVRCIHDHLPGHRHDHQRAPAKMREEEARKWLSVKRKDFA--RVRLVCFPYAGGNSNFYKTWDVKEAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRP-KFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHE-APPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSVI 376
            MGC SS     CC+P        P V  A E+     A P   +  PT SQ   +A                            R P  M E  A+KWL    K  A  +VR++C  +AG NSN +KTWD+K  E+V VELP RNAR+N+K I DV ++AKKI+ A   +GY+ES  PLV FGHSFGA++   +A+I++ +Y+YTPK  +  GC PLH R G P  +   +DDE++ S++D GG+TKEMA+++ +V+  L  +K D+L +D+YV++E A  L+ P+  +G   D R P  +  EW R+TT   + + FPGGHFFLK  E +V+A++  VI
Sbjct:   36 MGCGSS-----CCIP--------PYVSKAIEVHAA--AEPKKASDAPTDSQSTFKA--------------------------GPRRPV-MSEAMAQKWLLSNGKPVAGAKVRIICLGWAGANSNVFKTWDLKCGEVVKVELPSRNARFNDKPIHDVNILAKKISQACFVMGYLESTTPLVIFGHSFGAMIGLHLARIMKKEYDYTPKMCIFGGCRPLH-RWGFPMSYYKEDDDEMIQSMVDCGGMTKEMAKDKLLVSSVLAGIKMDHLCMDRYVFNEKAEKLEAPLWLIGGLDDPRSPPEQLPEWDRYTTCSIQIKTFPGGHFFLKEKEADVLAWLGKVI 332          
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|585101141|gb|EWM21085.1| (thioesterase [Nannochloropsis gaditana])

HSP 1 Score: 179.874 bits (455), Expect = 1.176e-49
Identity = 98/258 (37.98%), Postives = 144/258 (55.81%), Query Frame = 0
Query:  128 AKMREEEARKWLSVKR-KDFARVRLVCFPYAGGNSNFYKTWDV-KEAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVF--------------FGHSFGAIVSTLVAQILRNQYNYTPKHL-----VVSGCVPLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTT--KEFESRIFPGGHFFLK 362
            A M   EA++W   +  +  A+ R++C  +AGGNS+ Y+TWD+  E E+V VELPGRNAR  E  + + +L+  ++  A+  +GY+ S  P                 FGHSFGA++ T +AQ LR +     K +     +VSG  PL  R G    S  +DD ++  L+ MGGI  E+A +R+++  FLPA +GDY A+D YVY   P L   ++A   + D R  +   E W + TT  K+F   +FPGGHFF+K
Sbjct:   50 APMPLSEAKRWFVCRAPRPEAKYRMLCLAWAGGNSSIYRTWDLGSEVEVVAVELPGRNARVKETPLKNFHLLVSRLLHAMEVVGYLPSSAPSFLPSSTSPPPLKPLLLFGHSFGAMLVTHLAQALRERREDEGKSVDVPLVLVSGMAPLDKRGGSTGVSAMSDDAMIQYLVKMGGIPSEVAASRELMNVFLPAFRGDYTAIDNYVYTPLPALPFRLVAFAGENDARASKEIMEGWVKMTTHEKDFAVDVFPGGHFFIK 307          
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|299116155|emb|CBN76062.1| (fatty acid-ACP thioesterase [Ectocarpus siliculosus])

HSP 1 Score: 160.999 bits (406), Expect = 2.714e-43
Identity = 91/264 (34.47%), Postives = 141/264 (53.41%), Query Frame = 0
Query:  130 MREEEARKWLSVKR-KDFARVRLVCFPYAGGNSNFYKTWDVKEAEMV---TVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAG---RPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAP-----PLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLK-PLEKEVVAYVSSVIFRDL 380
            M      KW++ +R K  A +RL C  +AGG+++ +  W  K  E V    +ELPGR  R  E  +T V  I + +A AL  +G ++   P   FGHSFG++V   +A+ L+      P +L+VSGC P  +  G    P  S    D LV  L+ +GG+ +E+    ++V  FLP ++ DY A + Y Y   P     P+ CPI+ LG ++D R P+ + E W+  T  EF  ++F GGHFFL+   E+ V+ ++  ++   L
Sbjct:    1 MSSSALEKWVACRRVKADASIRLFCAAWAGGSASSFNKWTSKLPEAVELHALELPGRMRRSKEDSLTSVSAIVEGVAQALVTMGLLDK--PFALFGHSFGSLVMFELARKLKEDAGKIPVYLIVSGCCPPDMCVGGGSSPPISQLPRDALVDHLIGLGGMPEELRDQTELVDYFLPPIRADYTAYEGYRYELRPGDDSRPITCPIICLGGEEDGRAPRSDLERWSSLTAAEFRLQMFGGGHFFLQGEGEQAVLTFLGDILTEKL 262          
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|1154767560|ref|WP_078684186.1| (hypothetical protein [Desulfovibrio bizertensis] >gi|1152131839|emb|SKA67754.1| medium-chain acyl-[acyl-carrier-protein] hydrolase [Desulfovibrio bizertensis DSM 18034])

HSP 1 Score: 156.762 bits (395), Expect = 1.574e-41
Identity = 85/241 (35.27%), Postives = 132/241 (54.77%), Query Frame = 0
Query:  148 RVRLVCFPYAGGNSNFYKTWDV---KEAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCV-PLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSVIFRDLTGPG 384
            R+RLVC PYAGG ++ ++ W     ++ E++ V+LPGR  R  +   +D+ +I  K+ AAL    Y++   PL FFG+S G  ++  +A+ LR Q N  P  + V+ C  P       P++ +  D E   +L D+GG   E+  N +++  +LPAL+ D+   + YV+   PP DCPI A   D D   PQ E++ W + TT  F  R FPGGHFF+     E+++ + S +   L  P 
Sbjct:   19 RLRLVCLPYAGGGASLFRKWPQFLPEDIELLAVQLPGREKRLFDPPCSDLAVIVHKLRAALQN--YLD--CPLGFFGYSMGTRIAYELARTLREQDNIQPACMFVAACKGPQIEEPPNPRY-LLPDKEFKEALRDLGGTPDEVLENTELMDMYLPALRADFALKESYVHSPCPPFDCPITAYCGDHDKEAPQDESQTWDQETTAAFRLRCFPGGHFFIHDNLSELLSDMKSQLHITLREPS 254          
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|918244401|ref|WP_052375403.1| (thioesterase [Chondromyces apiculatus] >gi|599567444|gb|EYF05488.1| putative thioesterase involved in non-ribosomal peptide biosynthesis [Chondromyces apiculatus DSM 436])

HSP 1 Score: 155.992 bits (393), Expect = 3.327e-41
Identity = 88/248 (35.48%), Postives = 127/248 (51.21%), Query Frame = 0
Query:  133 EEARKWLSVKRKDFA-RVRLVCFPYAGGNSNFYKTWDVK---EAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSVI 376
            + + KWL   R   A RVRL+C PYAGG +  ++TW  K   + E++ ++LPGR  R  E ++  V  +   +   L      +S +P V FGHS GAI+S  + + LRNQ +  P  L+VSG    +L    P     +D  L+  +   GG  +E+ R  +++   LP L+ D+  +  + Y  A PL  P+LALG   D   P    E W      EF SRIFPGGHFFL   E  V+  ++  I
Sbjct:   23 DASSKWLWRSRPGAAPRVRLICVPYAGGGAASFRTWPAKLPADVELLAIQLPGRGMRMGEPLLRRVEQVVDAVGPLL-----TDSPVPFVLFGHSMGAILSFNLCRWLRNQGHPLPARLIVSGHRAPNLPDPEPPKHHLSDAALLDRIRRYGGTPEEILREPELMELMLPILRADFELLGTHAYTSAAPLPVPLLALGGADDPNAPPDTIEAWREHAAGEFSSRIFPGGHFFLHTEEAAVLPVIAEEI 265          
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|1121311269|ref|WP_073622413.1| (thioesterase [Calothrix sp. HK-06] >gi|1115279927|gb|OKH43166.1| putative thioesterase [Calothrix sp. HK-06])

HSP 1 Score: 154.836 bits (390), Expect = 5.468e-41
Identity = 80/248 (32.26%), Postives = 134/248 (54.03%), Query Frame = 0
Query:  133 EEARKWLSVKRKDFARVRLVCFPYAGGNSNFYKTWD---VKEAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSVIF 377
            + + +W+  +    A  R+ CFPYAG  ++ ++ W    + E E+  ++LPGR  R  E + T +  + + ++ AL  L Y++  +P  FFGHS GA+VS  VA+ L++ YN +PKHL VSG     LR+ +P      D+  +  L    G  + + +N ++++ FLP L+ D+   D Y  H   PL+CPI A G  QD  V   +   W   T  +F   +FPG HFF+K   ++++  ++  ++
Sbjct:    5 QTSTRWICNQPNRQASKRMFCFPYAGAGASVFRNWSNQLLPEVEICPIQLPGREGRMAEPLFTKLSPLVEALSKAL--LPYLD--LPFTFFGHSMGALVSFEVARFLQSHYNISPKHLFVSGRQAPQLRSSKPPIHQLPDNLFIEELRRYNGTPEMVLQNHELLSLFLPILRADFAINDTYT-HTNMPLNCPITAFGGLQDSGVSLTDIAAWRYQTNSDFSMHMFPGDHFFIKNEREQLLLLIAETMY 247          
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|1005323321|ref|WP_061608361.1| (thioesterase [Sorangium cellulosum] >gi|1005195667|gb|KYF69472.1| gramicidin dehydrogenase [Sorangium cellulosum])

HSP 1 Score: 154.451 bits (389), Expect = 8.067e-41
Identity = 90/250 (36.00%), Postives = 129/250 (51.60%), Query Frame = 0
Query:  138 WLSVKRKDFA-RVRLVCFPYAGGNSNFYKTWDVK---EAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSV---IFRDL 380
            W+   R + A R+RL CFPYAGG ++ Y  W  +     E+  ++LPGR AR +E     +  + + +A AL G        P  F+GHS GA+VS  +A+ LR +    P+HL VSG    H     P   +  DD  ++ L  MGG  +E+ R  D++  FLP L+ D    +   +   PPLDCPI A G D D R    + E W   T   F + +FPGGHFFL+     ++  +S+V   + RDL
Sbjct:   10 WVGCPRPNPASRLRLFCFPYAGGGASIYSAWWRELPAHTELCAIKLPGREARLSEPPFERLTPLVQALATALDGW----LAKPFAFYGHSLGALVSFELARELRRRGAPLPRHLFVSGRRAPHQPISAPLHGLP-DDAFLAWLRRMGGTPEEVLREPDLLALFLPVLRADVAVNEAEPFVPGPPLDCPISAFGGDDDERASPADIEAWRELTRGPFRAEVFPGGHFFLRTARAPLLRSISAVLDDVARDL 254          
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|1124241371|ref|WP_074541226.1| (thioesterase [Bacillus cereus] >gi|1152964868|gb|OOZ79399.1| thioesterase [Bacillus cereus])

HSP 1 Score: 153.68 bits (387), Expect = 1.533e-40
Identity = 76/234 (32.48%), Postives = 135/234 (57.69%), Query Frame = 0
Query:  149 VRLVCFPYAGGNSNFYKTWDVK---EAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSVIFRD 379
            +RL CF YAGG+++ +K W ++   + E++ V+LPGR  R  EK   ++  I  ++A  +  L       P VFFGHS GA++S  ++  L+  ++ +P+ L++S     HL          +    +  L +MGG ++E+ RN++++   +P ++ D+   +KYVY E   L+CPI+A G  +D RV     ++W + T+K F+ ++FPG HF+LK +EKE++  +S  +  +
Sbjct:   24 MRLFCFHYAGGSASNFKKWSIELPDQVEVIAVQLPGRENRIMEKPFNNIQSIICELAVEILPL----LDKPYVFFGHSMGALLSYEMSWYLKENFSTSPEKLIISSFRAPHLSPITNNMHDLSVPIFIEKLKEMGGTSEEILRNQELMELIIPTIRADFAVCEKYVYKERSKLNCPIVAYGGTEDSRVRLEHLKQWEKHTSKAFKVQMFPGNHFYLKEVEKELLYLISKELLEN 253          
BLAST of EWM29817.1 vs. NCBI_GenBank
Match: gi|1005199749|gb|KYF72840.1| (gramicidin dehydrogenase [Sorangium cellulosum])

HSP 1 Score: 153.295 bits (386), Expect = 2.245e-40
Identity = 89/250 (35.60%), Postives = 130/250 (52.00%), Query Frame = 0
Query:  138 WLSVKRKDFA-RVRLVCFPYAGGNSNFYKTWDVK---EAEMVTVELPGRNARYNEKVITDVYLIAKKIAAALAGLGYIESKIPLVFFGHSFGAIVSTLVAQILRNQYNYTPKHLVVSGCVPLHLRAGRPKFSVANDDELVSSLLDMGGITKEMARNRDVVTQFLPALKGDYLAVDKYVYHEAPPLDCPILALGSDQDLRVPQHETEEWARFTTKEFESRIFPGGHFFLKPLEKEVVAYVSSV---IFRDL 380
            W+   R + A R+RL CFPYAGG ++ Y  W  +     E+  ++LPGR AR  E     +  + + +A AL G        P  F+GHS GA+VS  +A+ LR +    P+HL VS     HL    P   + +DD+ ++ L  MGG  +E+ R+ D++  FLP L+ D    +   +   PPLDCP+ A G   D R    E E W   T   F + +FPGGHFFL+     ++  +S+V   + RDL
Sbjct:   10 WVGCPRPNPASRLRLFCFPYAGGGASVYSAWWRELPAHTELCAIKLPGREARLGEPPFERLTPLVQALATALDGW----LAKPFAFYGHSLGALVSFELARELRRRGAPLPRHLFVSARRAPHLPIAAPLHGL-SDDDFLAWLRRMGGTPEEVLRDPDLLALFLPVLRADVAVNEAEPFVPGPPLDCPMSAFGGADDERASPAEMEAWREHTRGPFRTEVFPGGHFFLRTARAPLLRSISAVLDGVARDL 254          
The following BLAST results are available for this feature:
BLAST of EWM29817.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585112398|gb|EWM29817.1|0.000e+0100.00microcystin synthetase-associated thioesterase [Na... [more]
gi|585100986|gb|EWM20990.1|3.077e-7240.59microcystin synthetase-associated thioesterase [Na... [more]
gi|585101141|gb|EWM21085.1|1.176e-4937.98thioesterase [Nannochloropsis gaditana][more]
gi|299116155|emb|CBN76062.1|2.714e-4334.47fatty acid-ACP thioesterase [Ectocarpus siliculosu... [more]
gi|1154767560|ref|WP_078684186.1|1.574e-4135.27hypothetical protein [Desulfovibrio bizertensis] >... [more]
gi|918244401|ref|WP_052375403.1|3.327e-4135.48thioesterase [Chondromyces apiculatus] >gi|5995674... [more]
gi|1121311269|ref|WP_073622413.1|5.468e-4132.26thioesterase [Calothrix sp. HK-06] >gi|1115279927|... [more]
gi|1005323321|ref|WP_061608361.1|8.067e-4136.00thioesterase [Sorangium cellulosum] >gi|1005195667... [more]
gi|1124241371|ref|WP_074541226.1|1.533e-4032.48thioesterase [Bacillus cereus] >gi|1152964868|gb|O... [more]
gi|1005199749|gb|KYF72840.1|2.245e-4035.60gramicidin dehydrogenase [Sorangium cellulosum][more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna840rna840Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications