EWM22415.1, cds8219 (CDS) Nannochloropsis gaditana

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|585102921|gb|EWM22415.1| (5-methyltetrahydropteroyltriglutamate--homocysteine s-methyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 1399.8 bits (3622), Expect = 0.000e+0
Identity = 677/677 (100.00%), Postives = 677/677 (100.00%), Query Frame = 0

Query:    1 MSADNVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGTVPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQASTTCTPPLFLAWNPWQLRRAHTWRRASTSPKSGLTRTVDSRLVAGRKFSPLCVTWWPQQSWFAKRWGEARPTPEVSPPPSKTLGLVVCFTPLLSALCVLERKGSRHM 677
            MSADNVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGTVPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQASTTCTPPLFLAWNPWQLRRAHTWRRASTSPKSGLTRTVDSRLVAGRKFSPLCVTWWPQQSWFAKRWGEARPTPEVSPPPSKTLGLVVCFTPLLSALCVLERKGSRHM
Sbjct:    1 MSADNVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGTVPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQASTTCTPPLFLAWNPWQLRRAHTWRRASTSPKSGLTRTVDSRLVAGRKFSPLCVTWWPQQSWFAKRWGEARPTPEVSPPPSKTLGLVVCFTPLLSALCVLERKGSRHM 677          

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|585102922|gb|EWM22416.1| (5-methyltetrahydropteroyltriglutamate--homocysteine s-methyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 1176.77 bits (3043), Expect = 0.000e+0
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0

Query:    1 MSADNVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGTVPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA 570
            MSADNVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGTVPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA
Sbjct:    1 MSADNVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGTVPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA 570          

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|760438007|ref|XP_011396072.1| (5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase [Auxenochlorella protothecoides] >gi|675350762|gb|KFM23202.1| 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase [Auxenochlorella protothecoides])

HSP 1 Score: 663.3 bits (1710), Expect = 0.000e+0
Identity = 336/575 (58.43%), Postives = 415/575 (72.17%), Query Frame = 0

Query:    1 MSADNVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELS-GLDAYFAAARG-HPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSR--GDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGT-VPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA 570
            M +  + ++TIG+PRIGP REMKKALE +W+GKS+ + L  VA+Q E  AW +Q D+G++LVALD T YDQ+LD  TYLGL+P RFQ L  G   YFA ARG H  +  ALDM K  D+NYHY+VPEL A+   + +W+  L  V RGQA +G++ AVP+LIGPIT   L+R     D S ++  L PAY  VL  L  LGVPEVQ+HEPVLAL  AN+ QAD E+ Y  LAG  VPL+LVV YDDV  DVY WLV+LPVQAI LDFC VPGA HG +T   IA+ GFP DKRLG GI+DGRS W D+GTAV ++AALR  LGP QA+ VQ+SA L H+P D+  E  LP  + +RLAFA QKLEE+VAV ++ +G A  A    L T+  A         I    + R   +A RRP+QPQF  FPTT+IGSFPQT  IRRARLQYK+G IS  +Y+E+I  EIGY+IG Q+AL +DV VHGE ER+DMVEYFG+KL+G+ FT+H WVQSYGSRYVRPP++ G+V+R GPMTVHE+ LAQ++T+R  VKGMLTGP T++NWSFPRKD+ RS QA
Sbjct:    1 MVSTQISSATIGFPRIGPNREMKKALESFWSGKSTADDLRTVAEQTERVAWLSQKDAGLDLVALDSTYYDQILDFITYLGLIPKRFQHLEPGFAQYFAMARGVHGAH--ALDMQKFFDTNYHYMVPELEASPAVEADWSGLLAKVERGQAVLGSDVAVPILIGPITLVSLARYPEGTDASALVGALLPAYVEVLGRLKALGVPEVQMHEPVLALGTANELQADFESSYSQLAGVGVPLNLVVYYDDVEEDVYSWLVRLPVQAISLDFCAVPGAAHGCATAATIARLGFPEDKRLGAGIVDGRSIWRDDGTAVDLLAALRARLGPNQAIVVQSSAPLLHLPYDVRAEKNLPGELTARLAFALQKLEEIVAVAKSGSGTAPPA----LETFGAAFPSDEGRSGIDASKYHRSEDYATRRPKQPQFRPFPTTSIGSFPQTEQIRRARLQYKRGIISTEQYKEQIGVEIGYAIGLQDALDIDVPVHGEPERSDMVEYFGLKLDGYSFTEHGWVQSYGSRYVRPPIVSGNVTRAGPMTVHEYRLAQAVTARP-VKGMLTGPVTMLNWSFPRKDVARSVQA 568          

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|552840366|ref|XP_005850025.1| (hypothetical protein CHLNCDRAFT_141995 [Chlorella variabilis] >gi|307109686|gb|EFN57923.1| hypothetical protein CHLNCDRAFT_141995 [Chlorella variabilis])

HSP 1 Score: 614.379 bits (1583), Expect = 0.000e+0
Identity = 320/568 (56.34%), Postives = 395/568 (69.54%), Query Frame = 0

Query:    5 NVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGT-VPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHA-GAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA 570
             + T TIG+PRIG  REMKKALE YWAG +  +AL   A  VE  AW+ QA +GI L+ALD T YD  L   +  G   A     + L  YFA ARG      A DMSK  DSNYH++VPEL   S P+P+W+  L+ V+RGQ  VG E+AVPM++GP T   L+RG FDRS ++ RL PAY  +L +LA+LGVPEVQIHEP+L +S +    AD    Y  LA   +P+++VV YDD   + Y WLV+LPV A+ LDF GVPGA +G+ST Q IAK+GFP  KRLG G+IDGR  WAD G A ++VA+LR  LG  Q   + TS SLQHVP DL  E   P  +   LAFA QKL E+V    AV G A GA   +DL+ +TGAP  G   + +P + F+RP P+A RRP+Q Q+  FPTTTIGSFPQTP IRR+RL +KKG +S+ +YRE++A EIG  IG QEALGLDVLVHGE ER+DMVEYFG+KL G+ FT+  WVQSYGSRYVRPP++ GD+SR GPMTVHE+ELAQ +TS+  VKGMLTGP TI+ WSFPR+D TR +QA
Sbjct:    3 TISTCTIGFPRIGSNREMKKALEAYWAGSNDAQALLQAAAGVEADAWRCQARAGIQLIALDSTLYDHALQERSATGAP-AAVPGAAALPVYFAMARG-AEGAPACDMSKHFDSNYHHIVPELDEQSEPRPDWSLLLDRVKRGQEAVGRERAVPMVVGPSTLLGLARGSFDRSSLLARLAPAYAQLLRQLAELGVPEVQIHEPILTMSGSEGLAADCAACYAELAAVGLPINIVVAYDD---ETYKWLVELPVAALSLDFLGVPGAEYGSSTAQHIAKHGFPKSKRLGAGVIDGRGIWADQGQAARLVASLRALLGEEQ--RIATSTSLQHVPYDLAAEEVPPAGLARPLAFAVQKLGEVVGTAAAVLGAAPGAIPALDLAAFTGAP--GGAPRELPADMFRRPEPYAERRPKQVQYPPFPTTTIGSFPQTPAIRRSRLAFKKGRLSQEQYREKMAGEIGLCIGVQEALGLDVLVHGEPERSDMVEYFGLKLGGYHFTEQGWVQSYGSRYVRPPIVTGDISRSGPMTVHEYELAQGLTSKP-VKGMLTGPVTILQWSFPRRDATRRQQA 560          

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|545374437|ref|XP_005651506.1| (cobalamin-independent methionine synthase [Coccomyxa subellipsoidea C-169] >gi|384253487|gb|EIE26962.1| cobalamin-independent methionine synthase [Coccomyxa subellipsoidea C-169])

HSP 1 Score: 597.045 bits (1538), Expect = 0.000e+0
Identity = 315/584 (53.94%), Postives = 392/584 (67.12%), Query Frame = 0

Query:    6 VKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSR-----GDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGT-VPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMV---------AVKEAVTGHAGAASDIDLSTYTGAPTMGLTA----KAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA 570
            + TSTIG+PRIG KRE+KKALE YW  ++S E L AV  + +  AW  QAD+GI+LV LDGT YDQ+L+   YLGL P+RF  LSG D YFA ARG  P   ALDMSK  D+NYHYLVPEL    TPKP+++   + V+RGQ  +G EKAVP++IGP T   LS+       FD    I+ L PAY  +L +L  LGVPEVQ+HEP+L  S A + +A+ E+ Y  L+   VP++LV  YDD+  + Y W VKLPV A+ LDF GVPG+  GN T  L+ KYGFPSDKRLG G++DGRS +AD  T   +VAAL K       +SVQ+S SLQH+P + +LETELP  + +RLAFA+QK+ E+V         +   ++T    A S++       A T GL      +AI    F R   F  RR +Q Q   FPTTTIGSFPQTP +RRARLQ+KKG +S  +Y + I   + Y+   QE +GLDV VHGE ERTDMVEYFG+KL GF FT+  WVQSYGSRYVRPP+I GDVSR GP+TV EF+ AQS+T +  VKGMLTGP TI+NWSFPRKDI+RS QA
Sbjct:    3 ITTSTIGFPRIGEKREVKKALETYWKKQTSLEELLAVNNEAQRLAWTLQADAGISLVGLDGTLYDQVLEFIFYLGLAPSRFSHLSGYDLYFALARG-VPGTEALDMSKFFDTNYHYLVPELATTVTPKPDFSLLFDKVKRGQDAIGKEKAVPIIIGPNTLVGLSKPADASAPFDHDSAIKALLPAYTELLTQLKALGVPEVQLHEPILTTSDAAKLKAEFESTYSELSKVGVPINLVTYYDDIG-EAYEWAVKLPVAAVSLDFLGVPGSALGNETAALVEKYGFPSDKRLGAGVVDGRSVFADGDTPANLVAALLKK--GITNISVQSSTSLQHLPYNKDLETELPADLVARLAFAKQKVAEIVEAASKAPSASAPASITAALPAVSELQ------ACTHGLDVYDPKEAIAENLFNRSEEFKARRSKQIQTPDFPTTTIGSFPQTPELRRARLQFKKGQLSAEDYEKVINDYMKYANDFQERIGLDVFVHGEPERTDMVEYFGVKLEGFAFTEAGWVQSYGSRYVRPPIIYGDVSRTGPITVKEFKYAQSLTQKP-VKGMLTGPVTILNWSFPRKDISRSAQA 575          

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|1013287104|dbj|BAU71146.1| (METE; cobalamin-independent methionine synthase [Parachlorella kessleri])

HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0
Identity = 333/578 (57.61%), Postives = 401/578 (69.38%), Query Frame = 0

Query:    8 TSTIGYPRIGPKREMKKALEEYWAG---KSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTP-KPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGT-VPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPS--DKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLD-LNLETELPTAVKSRLAFAQQKLEEMVA-VKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQ----------EALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITR 566
            + TIG+PR GPKREMKKALE YWA    K +KE L A A +VE  AW+ QA++G++LV LDGT YDQ+LD   YLGLLP RFQ LSGLD YFAAAR   P   A DMSK  D+NYHYLVPELT  S P  P+W+  L+ V RGQA VG EKAVP+L+GP T   L++GDF RS ++ RL PAY+ +L  L  LGVPE  IHEP+L        +AD    Y SLAG  VP++LVV YDDV+   Y WLV+LPV A+ LDF GVPG   G  T +LIAK+GFP   DKRLG G+IDGR+ WAD G A  ++AALR  LGP Q + +QTS  LQHVP D  + E  LP A+  R+AFAQQK +E+   V    T   G+   ++L  +TGA       +  P  FF+  A +  RR +QP F  FPTTTIGSFPQTP IRRARLQ+KKG +S  EYRER+AAEIG++IGAQ          EALGLDVLVHGE ER+DMVEYFG+KL GF FT++ WVQSYGSRYVRPP+I GDVSR  PMTVHE+ +AQ +T++  VKGMLTGP TI+ WSF R D++R
Sbjct:    5 SGTIGFPRTGPKREMKKALELYWANPAAKGAKETLLATAAEVEAQAWRCQAEAGVDLVGLDGTLYDQVLDFSHYLGLLPKRFQHLSGLDRYFAAAR-GAPGAPAADMSKYFDTNYHYLVPELTPESGPTSPDWSLLLDRVARGQAVVGVEKAVPILLGPNTLVGLAKGDFARSEVVARLVPAYRQLLETLKGLGVPE--IHEPILTRDDGATLEADFGACYASLAGAGVPINLVVYYDDVSEATYKWLVQLPVAAVSLDFKGVPGNDQGCHTARLIAKHGFPQARDKRLGAGLIDGRAIWADQGEAAAMLAALRARLGPDQRICLQTSTPLQHVPYDKRSEEGHLPAALLCRIAFAQQKAQELAGTVATLRTSPPGSIPALNLGLWTGAAPTPAELQFKPELFFRSEA-YEGRRGRQPCFPPFPTTTIGSFPQTPAIRRARLQFKKGALSPEEYRERMAAEIGFAIGAQASKGAECPLVEALGLDVLVHGEPERSDMVEYFGLKLAGFHFTENGWVQSYGSRYVRPPIIAGDVSRLEPMTVHEYLIAQGLTTKP-VKGMLTGPVTILQWSFVRTDVSR 577          

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|545706609|ref|XP_005705621.1| (5-methyltetrahydropteroyltriglutamate--homocysteinemethyltransferase [Galdieria sulphuraria] >gi|452822078|gb|EME29101.1| 5-methyltetrahydropteroyltriglutamate--homocysteinemethyltransferase [Galdieria sulphuraria])

HSP 1 Score: 560.066 bits (1442), Expect = 0.000e+0
Identity = 304/571 (53.24%), Postives = 381/571 (66.73%), Query Frame = 0

Query:    5 NVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVAL-DGTCYDQMLDHCTYLGLLPARFQELSG-LDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGD-FDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGT-VPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQF-HAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA 570
            N  T+TIGYPRIG  REMKKALE YW GKSS + L  V+KQVEE + K Q ++GI L+ + D T YD +LD    LGL+P RF+++    D YFA ARG+   + ALDMSK  DSNYHYLVPEL A+S P P++  FL+ + R Q  +G E+AVP++ GP+T+  L+R +      MI RL PAY ++LN L  L VPEVQ+HEP   L  A   Q  AE  YQ LA   VP++LV  YDD+    YPW+VKLPVQ I +DF        G++   L+ K+GFP+DK+LG G++DGRS W D G A +V+  L   +    A+ VQ S SL HVPLD+N E  L +++KSRL+FA+QK+ E+  V       A   SD   S  +GA +  L A  +    F R  PF+ RR QQ      F TTTIGSFPQTP +R+ RLQYKKG +SE EY+ +I   I ++IG QEALGLDV VHGE+ER+DMVEYFG KL GF FT++ WVQSYGSRYVRPP+I  DVSR  PMTV EF +AQSMT++  VKGMLTGP TI+NWSFPRKDI R +QA
Sbjct:    3 NKATATIGYPRIGLNREMKKALEAYWGGKSSLDELLKVSKQVEEESLKDQMNAGIQLIGVGDHTMYDHVLDWTFRLGLIPDRFKQVQNPKDKYFAMARGNKEGLQALDMSKFFDSNYHYLVPELNAHSKPSPSFEEFLDEISRAQQQIGKERAVPIVFGPVTYARLARCEGVSVDEMISRLVPAYVSLLNSLKSLQVPEVQLHEPGFVLGDAETLQKAAEAAYQELAKVQVPINLVTYYDDLGK-TYPWVVKLPVQTISMDFV------RGDN-LSLVKKHGFPADKKLGAGVLDGRSVWKDTGYAAQVIKELEAIVK--SAIVVQPSCSLMHVPLDVNSEPSLDSSLKSRLSFARQKIAELGVV-------ASGKSDGASSGSSGATSQDLEA-TLDKSMFSRCEPFSKRRSQQFTVPGGFGTTTIGSFPQTPAVRKLRLQYKKGQVSEEEYQRQIDRHISFAIGVQEALGLDVFVHGESERSDMVEYFGQKLRGFTFTENGWVQSYGSRYVRPPIIYADVSRISPMTVREFVVAQSMTNKP-VKGMLTGPVTILNWSFPRKDIPRKQQA 554          

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|159489910|ref|XP_001702934.1| (cobalamin-independent methionine synthase [Chlamydomonas reinhardtii] >gi|158270957|gb|EDO96787.1| cobalamin-independent methionine synthase [Chlamydomonas reinhardtii])

HSP 1 Score: 551.206 bits (1419), Expect = 0.000e+0
Identity = 308/601 (51.25%), Postives = 391/601 (65.06%), Query Frame = 0

Query:    3 ADNVKTSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPEL--------TANSTP-KPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRG-DFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLA---GTVPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQA----LSVQTSASLQHVPLDLNLETE----------LPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASD------IDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA 570
            A  + T+TIG+PRIG +R++K A+E Y+ G S +  L AVA +V+  AW  Q  +GI ++ LDGT YDQ+LD  T+LG +P RF+ LSGL  Y+A ARG      ALDMSK  D+NYHYLVPEL        TA   P +P+++  L+ + RGQA VG E+AVP+LIGP+TF  LSRG +      + RL P Y  +L +LA  G PEVQ+HEPVLA S     +A+ ET Y  +A   G+VPLHLV  YDD+ A  YPW V+LPV A+ LDF G PGA   + T  L+ ++GFP+DKRLG G++DGRS W D+GTAV   A LR  L  G      L V +SA LQH+P DL LE E          LP A+ +RL FA+QK+EE+V+V       A AA+       + L        +      IP E+F RP P+ VRR QQ Q  AFPTTTIGSFPQT  +RR R Q K G +++ EY   IA  I +++G QEALG+DVLVHGEAERTDMVEYFGM+L G  FT+  WVQSYGSR VRPPL+V D++ +GPMT  E+++A + T R  VKGMLTGP TI+NWSFPRKDI+R+ QA
Sbjct:    2 AAMLSTTTIGFPRIGNQRQLKFAMESYFKGDSGEAELLAVAHKVQSDAWALQKAAGIAVIGLDGTLYDQVLDTITWLGAIPPRFKHLSGLQRYYAMARGG----AALDMSKFFDTNYHYLVPELGPDVLGPATAAGAPLQPDFSGPLDKLARGQAVVGRERAVPILIGPVTFVSLSRGCELPLDQAVARLLPTYCALLQQLAAAGAPEVQLHEPVLATSEGAGMRAEFETAYAQMAQAAGSVPLHLVTYYDDLGA-AYPWAVQLPVAAVTLDFLGPPGAAVPSQTLALLQQHGFPADKRLGAGVVDGRSVWKDDGTAV---ALLRALLDTGAVSSDRLVVTSSAPLQHLPYDLGLELEAPKTPEAEPHLPPALAARLGFAKQKVEEIVSVARLAASPAAAAAAAGHGAVVQLQRLQQGKGVEDHTADIPAEWFSRPKPYDVRREQQLQLPAFPTTTIGSFPQTAEVRRLRQQLKSGRLTQAEYEGLIAGHIAHAVGVQEALGIDVLVHGEAERTDMVEYFGMQLGGMLFTRAGWVQSYGSRCVRPPLVVDDITYRGPMTCWEYKVASAYT-RKPVKGMLTGPVTILNWSFPRKDISRAAQA 593          

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|926799059|ref|XP_013906953.1| (5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase [Monoraphidium neglectum] >gi|761978265|gb|KIZ07934.1| 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase [Monoraphidium neglectum])

HSP 1 Score: 537.724 bits (1384), Expect = 3.823e-179
Identity = 286/564 (50.71%), Postives = 370/564 (65.60%), Query Frame = 0

Query:    8 TSTIGYPRIGPKREMKKALEEYWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRGDFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLAGT-VPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLETELPTAVKSRLAFAQQKLEEMVAVKEAVTGHAGAASDIDLSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA 570
            ++TIG+PR G KR+ K ALE +W GK+S+  L A    V+E AW+ Q  +G+ L+ALDGT YDQ+LD    LG+ PARF+ LSGL+ YFA ARG P N  ALDMSKL+D+NYHYLVPEL     PKP+++  L+ ++RGQA +G + AVPM++GP+TF +LS        ++++L P+Y  +L +L  +G PEVQ+HEP LA       Q  A+  Y  LA    P+HLV  YDD+  + YPW+ +LPV+A+ LDFCGVPGA   N+T  LI   G+P+DKRLGVG +DGRS WAD+  A   +    + LG   +L+V +S SLQH+P D+ LE  LP  +  RLAFA QKL    A+     G   + +      + G   +  +A  +    F R   F  RR +QPQF AFPTT+IGSFPQT  +RR R Q K G I    Y   I  +I  +IG QE L +DVLVHGEAERTDM E+FGM+L+G  FTQH WVQSYGSRYVRPP+I GDV+    MTV EF++AQ +T R  VKGMLTGP TI+NWSFPRKD++R  QA
Sbjct:    4 STTIGFPRTGSKRQAKVALESFWKGKTSEPDLVASFAAVDEEAWRAQQAAGVQLIALDGTYYDQVLDMVYALGVAPARFKHLSGLELYFAMARGAP-NAPALDMSKLLDTNYHYLVPELEEGFAPKPDFSAILDRLKRGQALLGKDAAVPMMVGPVTFALLSSTTLPIESVVEKLLPSYAQLLRQLQAVGAPEVQLHEPALATDKGAAAQRAAQAAYAQLAAVGCPIHLVACYDDLG-EAYPWVAQLPVRALTLDFCGVPGATAPNATLDLIRANGWPADKRLGVGCVDGRSVWADDEGACTALLNELRTLG-ASSLAVTSSVSLQHLPWDVTLEPALPAGLAPRLAFAVQKLG---AIARLAAGKGVSGAAGAAGGWVG--RVRDSAMELERAMFDRAENFEARRAKQPQFPAFPTTSIGSFPQTAQVRRLRAQLKSGAIDRPTYERLIDQQIALAIGIQEGLDIDVLVHGEAERTDMSEHFGMQLSGMVFTQHGWVQSYGSRYVRPPIIAGDVAFVNAMTVREFKVAQDLTPRP-VKGMLTGPVTILNWSFPRKDLSRRAQA 558          

BLAST of EWM22415.1 vs. NCBI_GenBank
Match: gi|1004148829|gb|KXZ56801.1| (METE protein [Gonium pectorale])

HSP 1 Score: 525.013 bits (1351), Expect = 1.066e-173
Identity = 281/558 (50.36%), Postives = 361/558 (64.70%), Query Frame = 0

Query:   29 YWAGKSSKEALTAVAKQVEEAAWKTQADSGINLVALDGTCYDQMLDHCTYLGLLPARFQELSGLDAYFAAARGHPPNITALDMSKLMDSNYHYLVPELTANSTPKPNWAPFLEAVRRGQATVGAEKAVPMLIGPITFTVLSRG-DFDRSVMIQRLGPAYQTVLNELAKLGVPEVQIHEPVLALSAANQYQADAETVYQSLA-GTVPLHLVVPYDDVAADVYPWLVKLPVQAIGLDFCGVPGAPHGNSTCQLIAKYGFPSDKRLGVGIIDGRSPWADNGTAVKVVAALRKHLGPGQALSVQTSASLQHVPLDLNLET-ELPTAVKSRLAFAQQKLEEMVAVKEAV--------TGHAGAASDID-----LSTYTGAPTMGLTAKAIPTEFFQRPAPFAVRRPQQPQFHAFPTTTIGSFPQTPTIRRARLQYKKGTISEVEYRERIAAEIGYSIGAQEALGLDVLVHGEAERTDMVEYFGMKLNGFCFTQHAWVQSYGSRYVRPPLIVGDVSRQGPMTVHEFELAQSMTSRAFVKGMLTGPTTIINWSFPRKDITRSEQA 570
            +W GK S+  L + A +V+  AW+ Q D+GI  + LDGT YDQ+LD   +LG++PARF+ LSGL  YFA ARG P    ALDM+KLMD+NYH+LVPEL AN +P+PN+   ++ V +GQA +GA  A+P+++GP+TF  L++G        ++RL PAY  +L  LA + VPEVQ+HEP+LA S A   +   E  Y  LA   VPLHLV  YDD+ +  YPW V LPV ++ LDF GVPGA   N T QLI ++GFP+DKRLG G++DGRS WAD+GTAV +V  L     P + L + +SA LQH+P DL LE   LP  +  RLAFA QKL E+V+   AV        + H  A   +           G+       + +  + F R   +  RR  Q  F AFPTTTIGSFPQT  +RR R Q ++G +S  EY   I   I + +G QEALGLDVLVHGE+ERTDMVEYFG +L G   T   WVQSYGSR VRPP++VGD+  QGPM V E+++AQ+ T R  VKGMLTGP TI+NWSFPRKDI+R +QA
Sbjct:   18 FWQGKLSEADLLSTADKVQADAWELQRDTGIAAIGLDGTLYDQVLDTVQWLGVIPARFKGLSGLQRYFAMARGAPGR-PALDMTKLMDTNYHFLVPELDANFSPEPNYEWLVDKVAKGQAVLGAASAIPIVMGPVTFVCLAKGCPVPTDEAVRRLLPAYTELLRRLAGMRVPEVQMHEPILATSGAGDLRDSFEAAYLELAKAGVPLHLVTYYDDIGS-AYPWAVNLPVSSVTLDFLGVPGAAVPNQTLQLIRQHGFPADKRLGAGVVDGRSVWADDGTAVSLVTELLGLGIPHERLVITSSAPLQHLPYDLRLEGGHLPEGLAGRLAFATQKLAEIVSTATAVAVAAADPGSAHGDAPGAVKPQAGAAGAAAGSGEGVAFGQQLDEKLFSRSEAYKTRREAQATFPAFPTTTIGSFPQTAEVRRLRQQLQRGALSRPEYERLITGHIAHCVGVQEALGLDVLVHGESERTDMVEYFGTQLGGMWHTSSGWVQSYGSRCVRPPVVVGDIEYQGPMAVWEYQVAQTYTRRP-VKGMLTGPVTILNWSFPRKDISRQQQA 572