EWM22669.1, cds8051 (CDS) Nannochloropsis gaditana

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|585103234|gb|EWM22669.1| (dna polymerase theta [Nannochloropsis gaditana])

HSP 1 Score: 3076.19 bits (7974), Expect = 0.000e+0
Identity = 1528/1528 (100.00%), Postives = 1528/1528 (100.00%), Query Frame = 0

Query:    1 MGMESPLLEPVPVPLPTSTGAPLSSKELSPVSACEARGVKRGRNDGATRDTAACSKVIHPSSLRLPTPSDAPPVHDETLTEPRSDQKGLLMDDCTLQCENTLHSKRAEILQGNKPGKIDRLEEKGTSITILGPLTEGSDHERANDFSLPHADTPGSDGPRRYVSQRARERLVQGSLQHTAITVFSVEARSAHPPEKPPDQNATNLPSAQGPVLPSSPPNQSCRLSPPRDGNIPLAFVPVFPPRPAPAACLSAPSIPPSSSETLPPTLHLPGFERKTVSETRPVPKDPNDGSLRVKLGPSAPAPPPYRPTRTRRHEPTQPPSALASSISRQRGQDNVSGPKLLAQESSRASAVGQKQGQQTYLLPSAPPPVTTTVTTTRTKLSPKISTTAPQETCGDNEDWGGPAAAFNAAAIRGISRQGEGRGGKNGEPGGGGPRRPVEGLGIWRRGRGDPRKVGRGVGHRKGVLGNVEANRGSVKGPAGRGRGGRGNAMTYLALNGERLQGPAAMERHRQDTAKIQQGLTFTAQRSALRAALLARQPTAVGEGGVDGSSHGGVPIPPGRASTEETDAKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAACLGPWTEAVKVQEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGEGGGVERALLEVVMGRLAGCKEEEGGEGWLETFARCTLYAVQAHEEEGKEENGEGLGVEGQACKVLQRFRRGLDYLVQNRYIGRRSIAAVKEVSGSTVRDGETSGGSIVGPDESHLDPERGGGQGILQGANAAVDGTRPVPAATREHAGGAGPSLPTARPKTCYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPLLQLETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAAEHDSAKPRQGPKDGGRQGGGDKRVARRIADLIVRKAQEVARQKVNP 1528
            MGMESPLLEPVPVPLPTSTGAPLSSKELSPVSACEARGVKRGRNDGATRDTAACSKVIHPSSLRLPTPSDAPPVHDETLTEPRSDQKGLLMDDCTLQCENTLHSKRAEILQGNKPGKIDRLEEKGTSITILGPLTEGSDHERANDFSLPHADTPGSDGPRRYVSQRARERLVQGSLQHTAITVFSVEARSAHPPEKPPDQNATNLPSAQGPVLPSSPPNQSCRLSPPRDGNIPLAFVPVFPPRPAPAACLSAPSIPPSSSETLPPTLHLPGFERKTVSETRPVPKDPNDGSLRVKLGPSAPAPPPYRPTRTRRHEPTQPPSALASSISRQRGQDNVSGPKLLAQESSRASAVGQKQGQQTYLLPSAPPPVTTTVTTTRTKLSPKISTTAPQETCGDNEDWGGPAAAFNAAAIRGISRQGEGRGGKNGEPGGGGPRRPVEGLGIWRRGRGDPRKVGRGVGHRKGVLGNVEANRGSVKGPAGRGRGGRGNAMTYLALNGERLQGPAAMERHRQDTAKIQQGLTFTAQRSALRAALLARQPTAVGEGGVDGSSHGGVPIPPGRASTEETDAKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAACLGPWTEAVKVQEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGEGGGVERALLEVVMGRLAGCKEEEGGEGWLETFARCTLYAVQAHEEEGKEENGEGLGVEGQACKVLQRFRRGLDYLVQNRYIGRRSIAAVKEVSGSTVRDGETSGGSIVGPDESHLDPERGGGQGILQGANAAVDGTRPVPAATREHAGGAGPSLPTARPKTCYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPLLQLETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAAEHDSAKPRQGPKDGGRQGGGDKRVARRIADLIVRKAQEVARQKVNP
Sbjct:    1 MGMESPLLEPVPVPLPTSTGAPLSSKELSPVSACEARGVKRGRNDGATRDTAACSKVIHPSSLRLPTPSDAPPVHDETLTEPRSDQKGLLMDDCTLQCENTLHSKRAEILQGNKPGKIDRLEEKGTSITILGPLTEGSDHERANDFSLPHADTPGSDGPRRYVSQRARERLVQGSLQHTAITVFSVEARSAHPPEKPPDQNATNLPSAQGPVLPSSPPNQSCRLSPPRDGNIPLAFVPVFPPRPAPAACLSAPSIPPSSSETLPPTLHLPGFERKTVSETRPVPKDPNDGSLRVKLGPSAPAPPPYRPTRTRRHEPTQPPSALASSISRQRGQDNVSGPKLLAQESSRASAVGQKQGQQTYLLPSAPPPVTTTVTTTRTKLSPKISTTAPQETCGDNEDWGGPAAAFNAAAIRGISRQGEGRGGKNGEPGGGGPRRPVEGLGIWRRGRGDPRKVGRGVGHRKGVLGNVEANRGSVKGPAGRGRGGRGNAMTYLALNGERLQGPAAMERHRQDTAKIQQGLTFTAQRSALRAALLARQPTAVGEGGVDGSSHGGVPIPPGRASTEETDAKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAACLGPWTEAVKVQEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGEGGGVERALLEVVMGRLAGCKEEEGGEGWLETFARCTLYAVQAHEEEGKEENGEGLGVEGQACKVLQRFRRGLDYLVQNRYIGRRSIAAVKEVSGSTVRDGETSGGSIVGPDESHLDPERGGGQGILQGANAAVDGTRPVPAATREHAGGAGPSLPTARPKTCYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPLLQLETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAAEHDSAKPRQGPKDGGRQGGGDKRVARRIADLIVRKAQEVARQKVNP 1528          

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|1117413766|ref|XP_019382602.1| (PREDICTED: DNA polymerase theta isoform X2 [Gavialis gangeticus])

HSP 1 Score: 442.58 bits (1137), Expect = 2.589e-124
Identity = 306/945 (32.38%), Postives = 462/945 (48.89%), Query Frame = 0

Query:  555 PIPPGRASTEET--DAKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRAL--THAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAACLGPWTEAVKV------QEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGEGGGVE----RALLEVVMGRLAGCKEEEGGEGWLETFARCTLYAVQAHEEEGKEENGEGLGVEG--QACKVLQRFRRGLDYLVQNRYIGRRSIAAVKEVSGSTVRDGETSGGSIVGPDESHLDPERGGGQGILQGANAAVDGTRPVPAATREHAGGAGPSLPTARPKTCYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPLLQL-ETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAA 1482
            P P G+        D  + L    WG+P+ ++  Y  LGV  +F WQ  CL  GQ   + EG+NLVYSAPTS GKTLVAELL+L+ +  TH      ALF++PFV++A+EK  Y + ++  V + V+ +   A  A     + VAVCTIERAN L+NRL+E  ++  + +VVVDELHM+GD+ RG+L+E++L+KVR      ++    AS  A+G                              +QI+G+SATLPNL  +A+WL+A LY T+FRPV L   +  G  +               S+   +  ++P+ +L     DE   V+ LC E+V  GH VL+FC S+ W ++ A  IAR F        +   +      +EG  E++ +LR +  GL   L+ ++  GVAFHHAGLT +ER ++EG F+ G++  L ATSTL++GVNLPARRVI+R+ + F G+ + +  ++QM GRAGR G D  GE+IL+ + ++     A     L P+ S L   EG G+     RA+LE+++G +A   ++     W  T+A CTL      + E   E  E     G  +AC         + +L+QN +I         +VSG +                                     DG + V   T  H G A                     T  + +SP +A  + A L +A  KG +L +DLH+++L  P    + T DW+      ++    +  VA  +G+ E  + R V+ +                         +++++ +RF  SL L D++ EVPL  + +  G  RG LQ+LQ  A  + GM+ VF   L W  + +L+     RL   V  +L  L R+   L++A RAR L+  GF    D+A A   DV   L  ++PF +
Sbjct:   33 PSPSGQCQQMNIPEDQADKLLLASWGLPKAVLEKYHSLGVVHMFEWQAECLMLGQ---VLEGKNLVYSAPTSAGKTLVAELLILKRVLETHK----KALFILPFVSVAKEKKYYLQALFQEVGVQVEGYMGSASPAGRFSTLDVAVCTIERANGLINRLIEENQMDLLGMVVVDELHMLGDSHRGYLLELLLTKVRFITEKVTKRQAKASSPASGG-----------------------------IQIVGMSATLPNLHLLASWLNAELYHTNFRPVPLMEWVKIGSNI-------------YDSSMNLVREFQPMLQLKG---DEDH-VVSLCYETVRDGHSVLLFCPSKNWCEKLADIIAREFCNLQLSERKTSNLPPIPLNKEGIEEVMDQLRRSLSGLDSVLQRTLPWGVAFHHAGLTFDERDVIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPV-FGGKLLDILAYKQMAGRAGRKGADTVGESILVCKPSERSKGIALLQGSLKPVRSCLLRREGEGITSSMIRAILEIIVGGVASTPDDV----W--TYASCTLLVASLKDNEQGSEKNEDRVQNGAIEAC---------VAWLLQNEFI---------QVSGPS-------------------------------------DGVKEVYCPT--HLGSA---------------------TLSSSLSPMEALEIFADLQRA-MKGFVLENDLHIVYLVTPVYEEWTTIDWYQFFCLWEKLPTSMKRVAELVGIEEGFLARSVKGKIIAKTEK----------------QHRQMAIHKRFFTSLALLDLISEVPLKDITKKYGCSRGQLQSLQQSAATYAGMVTVFSNRLGWHNMEQLLSQFQSRLTFGVHRELCDLVRVS--LLNAQRARTLYNTGFVTVVDLAKANPADVETALKNTVPFKS 820          

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|831291624|ref|XP_012677360.1| (PREDICTED: DNA polymerase theta [Clupea harengus])

HSP 1 Score: 439.113 bits (1128), Expect = 3.473e-123
Identity = 316/958 (32.99%), Postives = 480/958 (50.10%), Query Frame = 0

Query:  541 VGEGGVDGSSHGGVPIPPGRASTEETDAKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAAC-----LGP--WTEAVKVQEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGEGGGVE----RALLEVVMGRLAGCKEEEGGEGWLETFARCTLYAVQAHEEEGKEENGEGLGVEGQACKVLQRFRRG----LDYLVQNRYIGRRSIAAVKEVSGSTVRDGETSGGSIVGPDESHLDPERGGGQGILQGANAAVDGTRPVPAATREHAGGAGPSLPTARPKTCYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPLLQL-ETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAA 1482
            + E G+  S H G+P       ++ TD K LLS   WG+P+ ++  Y+  GV+R+F WQ  CL   +G VL EG+NLVYSAPTS GKTLV+ELL+L+ +    +   A+F++PFV++A EK  Y + V+    + V+ +      A     + VAVCTIE+AN L+NRL+E   L  + +VVVDELHMVGD+GRG+L+E++L+K+R  A            H T  A            + G++V          +QI+G+SATLPNL  +A WLDA LY TD+RPV L   +  G  +                + A +  ++P  +    +  +   ++ LCLE+V +G  VL+FC S++W ++ A SIAR F        LGP         QEG  +++A+L+ +  GL + L+ +V+ GVAFHHAGLT +ER ++E GF+ G +  L ATSTL++GVNLPARRVI+R+ + F+G+ + +  ++QM GRAGR G+D  GE++L+ +E +     +     L P+ S L   EG GV     RA+LE+++G +A   EE      +  +A CTL  V +   EG          EG A K  +R R      +++L++N +I                                H+  ++             V G R  P     H G A  S   + P+         A + FA +             Q   KG +L +DLH+L+   P    + T DW+      ++    +  VA  +G+ E  + R V         +G L  + D          +++++ +RF  +LVL D++ EVP+  + +     RG LQ+LQ  A  + GM+ VFC  L W  L  L+     RL   V  +L  L R+   L++A  AR L+ AG     D A A+V DV  +L  ++PF +
Sbjct:  202 LAEAGL--SIHVGIP-------SDHTD-KLLLS--SWGLPKPVLERYQSRGVQRMFEWQAECL--TKGGVL-EGKNLVYSAPTSAGKTLVSELLILKRVLETRK--KAIFILPFVSVAREKMFYLQDVFQEAGVKVEGYMGSTSAAGGFGALDVAVCTIEKANGLINRLIEEDRLDLLGIVVVDELHMVGDSGRGYLLELLLTKIRFIA------------HKTSAA----------RSQNGSSV-------CKEIQIVGMSATLPNLDLLANWLDAELYHTDYRPVPLKEWVKIGTSI-------------YDGSMALVRHFQPNLQ----VKGDDDHIVSLCLETVQEGRSVLMFCPSKSWCEKLADSIAREFYNLHQKDNLGPSGLPPMSLNQEGLLDVVAQLKRSPAGLDQVLQRTVRWGVAFHHAGLTFDERDIIENGFREGCIRVLAATSTLSSGVNLPARRVIIRTPL-FNGRLLDILTYKQMVGRAGRKGVDTLGESVLVCKETERAKGVSLIQGTLKPISSCLVKQEGEGVTTSMLRAILEIIVGGVASTPEE------VRLYASCTLL-VASLAAEG--------ASEGSAAKEKERSRGAIEACIEWLMENEFI--------------------------------HIQKDKD------------VPGERYCPT----HLGSATLSSSLSPPE---------ALSIFADL-------------QRAMKGFVLENDLHILYQITPVYVDWTTIDWYQFFCLWEQLPSSMKRVAEMVGIQEGFLARSV---------SGKLVAKTDK-------QRRQMAIHKRFFTTLVLLDLVNEVPVGTVAKKYACSRGQLQSLQQSASTYAGMVTVFCNRLGWHNLEMLLSQFQSRLSFGVQRELCDLVRIS--LLNAQTARTLYSAGLLTVADTAHAQVADVEKMLRKAVPFKS 992          

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|32306445|gb|AAN39838.1| (DNA polymerase Q [Mus musculus])

HSP 1 Score: 436.417 bits (1121), Expect = 2.108e-122
Identity = 303/970 (31.24%), Postives = 472/970 (48.66%), Query Frame = 0

Query:  576 GWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAAC------LGPWTE---AVKVQEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALH-VGE-GGGVERALLEVVMGRLAGCKEEEGGEGWLETFARCTLYAVQAHE-EEGKEENGEGLGVEG-QACKVLQRFRRGLDYLVQNRYIGRRSIAAVKEVSGSTVRDGETSGGSIVGPDESHLDPERGGGQGILQGANAAVDGTRPVPAATREHAGGAGPSLPTARPKTCYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPLLQL-ETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAAEHDSAKPRQGPKDGGRQG-----GGDKRVARRIADLIVRKAQEVARQKV 1526
             WG+P+ ++  Y   GVR++F WQ  CL  G    + EG+NLVYSAPTS GKTLVAELL+L+ +    +   ALF++PFV++A+EK  Y + ++  V L V  +            + +AVCTIERAN LVNRL+E  ++  + +VVVDELHM+GD+ RG+L+E++L+K+          +  ++ H   +A  L  A                      VQI+G+SATLPNL  VA+WL+A LY TDFRPV L         LE ++       S   S+   +  ++PL +    +  +   ++ LC E++   H VL+FC S+ W ++ A  IAR F         L   +E    +  Q+   E++ +L+ +  GL   L+ +V  GVAFHHAGLT EER ++EG F+ G +  L ATSTL++GVNLPARRVI+R+ I F GQ + +  ++QM GRAGR G+D  GE+IL+ + ++     A     L P+ S L   GE    + RA+LE+++G +A   ++      ++T+A CT  A    E ++G + N +   +    AC         + +L++N +I                  G+ +GG +  P                                   H G A                     T  + +SP D   + A L +A  KG +L +DLH+++L  P    + + DW+      ++    +  VA  +GV E  + R V+ +           ER            +++++ +RF  SLVL D++ E+PL  + +  G  RG +Q+LQ  A ++ GMI VF   L W  +  L+     RL   +  +L  L R+   L++A RAR L+ +GF    D+A A   +V   L  S+PF +   +    +   +  R        G    AR  A LIV +A+ + +Q +
Sbjct:  115 NWGLPKAVLEKYHSFGVRKMFEWQAECLLLGH---VLEGKNLVYSAPTSAGKTLVAELLILKRVLETRK--KALFILPFVSVAKEKKCYLQSLFQEVGLKVDGYMGSTSPTGQFSSLDIAVCTIERANGLVNRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKI-------CYVTRKSASHQAESASTLSNA----------------------VQIVGMSATLPNLQLVASWLNAELYHTDFRPVPL---------LESIKIG----NSIYDSSMKLVREFQPLLQ----VKGDEDHIVSLCYETIQDNHSVLIFCPSKKWCEKVADIIAREFYNLHHQPEGLVKSSEFPPVILDQKSLLEVMDQLKRSPSGLDSVLKNTVPWGVAFHHAGLTFEERDIIEGAFRQGFIRVLAATSTLSSGVNLPARRVIIRTPI-FSGQPLDILTYKQMVGRAGRKGVDTMGESILVCKNSEKSKGIALLQGSLEPVHSCLQRQGEVTASMIRAILEIIVGGVASTSQD------MQTYAACTFLAAAIQEGKQGMQRNQDDAQLGAIDAC---------VTWLLENEFI-------------QVAEPGDGTGGKVYHPT----------------------------------HLGSA---------------------TLSSSLSPTDTLDIFADLQRA-MKGFVLENDLHIVYLVTPVFEDWISIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVART------ERQH----------RQMAIHKRFFTSLVLLDLISEIPLKDINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLIRVS--LLNAQRARFLYASGFLTVADLARADSAEVEVALKNSLPFKSARKAVDEEEEAAEERRSMRTIWVTGKGLSAREAAALIVEEAKMILQQDL 930          

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|999967256|gb|KXJ06430.1| (DNA polymerase theta [Exaiptasia pallida])

HSP 1 Score: 363.229 bits (931), Expect = 2.930e-106
Identity = 240/730 (32.88%), Postives = 378/730 (51.78%), Query Frame = 0

Query:  566 TDAKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRL--ERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAACLGP-----------WTEAVKV----QEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGE-GGGVERALLEVVM-GRLAGCKEEEGGEGWLETFARCTLYAVQAHEEEGKEENGEGLGVEGQACKVLQRFRRGLDYLVQNRYIGRRSIAAVKEVSGSTVRDGETSGGSIVGPDESHLDPERGGGQGILQGANAAVDGTRPVPAATREHAGGAGPSLPTARPKTCYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLF 1276
            +D  NL S   WG+P  +++ Y ++G+  +F WQ  CL  G   VLR G NLVYSAPTS GKT+VAELLML+ +        ALF++P+V++A EK  Y +R++    + V+ F      A     V +AVCTIE+AN+L+NRL+E  +++ + VVVVDE+HM+GD  RG+L+E+ L+K+R                 +G        AE +++ G +T       N + +Q++ +SATLPNL  +A WL A LY TD RPV L+ ++  G  +  +++ +    +P+KA                   + ++   ++ LC E+++QGH VL+FC ++ W ++ A++IAR F+    P           + +A  +     E   E+  +L+ T VGL   L   + HGVA+HHAGLT++ER ++EG F+ G +  LVATSTL +GVNLPARRVI+R+   F G+ +    ++QM GRAGR G+D+ GE++L+ +  +   A       L P+ S L + E  GG++RALLEV+  G + G ++       +E +A CT +A  + +++           +G  C + +  +    +LV N +I                              + + D E G    IL+     V   RP               L     +  Y  TQLG+AT  + +SP +A  +   L QA  K  +L S+LH+++
Sbjct:   35 SDVHNLAS---WGLPPSVLQRYHDMGITHMFEWQAECLRTGN--VLRGG-NLVYSAPTSAGKTMVAELLMLKRVLET--KRKALFILPYVSVAREKMFYLQRLFQEASVRVEGFMGSHNPAGGFTAVDIAVCTIEKANSLLNRLMEENKVSTLGVVVVDEMHMIGDPHRGYLLELFLTKIRF---------------VSGEGTVQGEQAECDNKDGKST-------NESPIQVVCMSATLPNLDMLARWLAADLYFTDHRPVPLTEMVKIGPTIYDKKMTKIRDIDPTKA-------------------VVNDDDHIIPLCKETITQGHSVLIFCPTKNWCEKQAEAIARHFSTIGNPGLARKTDNRSGFVKASDMIAFDYEAIREVFEQLKRTPVGLDSVLRRVLPHGVAYHHAGLTVDERDIIEGAFRQGTIRVLVATSTLCSGVNLPARRVIIRTPT-FHGKMVDPLVYKQMAGRAGRKGVDDLGESVLVCKTTERHKATTLMKSELKPVQSCLLLKELNGGIKRALLEVIASGVVTGLRD-------VERYAACTFFASSSRQDDX----------DGNKCAITETIK----FLVDNEFIRV----------------------------QVNKDKENGDSVSILR---FQVTSQRP------SWXNFXKGFLIKESAQERYVSTQLGSATVASALSPDEALVVFGDLQQA-RKCFVLESELHIIY 655          

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|706148562|ref|XP_010205958.1| (PREDICTED: DNA polymerase theta, partial [Colius striatus])

HSP 1 Score: 370.933 bits (951), Expect = 4.279e-101
Identity = 230/605 (38.02%), Postives = 342/605 (56.53%), Query Frame = 0

Query:  560 RASTEETDAKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAACLGPWTEAVKV--------QEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGEGGGV----ERALLEVVMGRLAGCKEEEGGEGWLETFARCTLYAVQAHEEEGKEENGEGLGVEG--QACKVLQRFRRGLDYLVQNRYI 1150
            + +T E  A  LL    WG+P+ ++  Y  LGV ++F WQ  CL  GQ   + EG+NLVYSAPTS GKTLVAELL+L+ +    +   ALF++PFV++A+EK  Y + ++  VD+ V+ +      A     + VAVCTIE+AN+L+NRL+E   +  + VVVVDELHM+GD+ RG+L+E++L+KVR             ++  T   V+     + N   GG             +QIIG+SATLPNL  +A+WLDA LY T+FRPV L   +  G  +               S+   +  +EP  +L     DE   V+ LC E+V +GH VL+FC S+ W ++ A  IAR F +     + A K         +EG NE+L +L+ +  GL   L+ ++  GVAFHHAGLT +ER ++EG F+ G++  L ATSTL++GVNLPARRVI+R+ + F G+ + V  ++QM GRAGR G+D  GE+IL+ + ++     A     L P+ S L   EG GV    +RA+LE+++G +A   ++      ++T+A CTL A    E +   E  +     G  +AC         + +L++N +I
Sbjct:    6 QVNTPEDQADKLL-LASWGLPKAVLEKYHSLGVVQMFEWQAECLMLGQ---VLEGKNLVYSAPTSAGKTLVAELLILKRVLETRK--KALFILPFVSVAKEKKCYLQALFQEVDVRVEGYMGSTSPAGRFSALDVAVCTIEKANSLINRLIEENNMDSLGVVVVDELHMLGDSHRGYLLELLLTKVR-----------YVTEKVTKRQVK-----KTNPSFGG-------------IQIIGMSATLPNLGLLASWLDAELYCTNFRPVPLKEWVKIGSNI-------------YDSSMNLVREFEPKLQLK---GDEDH-VVSLCYETVCEGHSVLLFCPSKNWCEKLADIIAREFYSLQQDESSAKKSALAPVVVDREGINEVLDQLKRSISGLDSVLQRTLPWGVAFHHAGLTFDERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPM-FGGKLLDVLTYKQMAGRAGRKGVDTEGESILVCKPSERSKGTALLQGYLKPVRSCLLRREGEGVSSSMKRAILEIIVGGVANTPDD------VQTYASCTLLASSLKESQWGNEKAKDKVQTGPIEAC---------VAWLLENEFI 542          

HSP 2 Score: 118.242 bits (295), Expect = 9.637e-23
Identity = 71/236 (30.08%), Postives = 114/236 (48.31%), Query Frame = 0

Query: 1248 PRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPLLQL-ETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAA 1482
            P +A  + A L +A  K  +L +DLH+++L  P    +   DW+      ++    +  VA  +G+ E  + R ++ + T                       +++++ +RF  SL L D++ EVPL  + E  G  RG LQ+LQ  A  + GM+ VFC  L W  +  L+     RL   V  +L  L R+   +++A RAR L+ AGF    D+A A  +DVA  L  S+PF +
Sbjct:  572 PTEAMEIFADLQRAM-KSFVLENDLHIVYLVTPVYEDWTIIDWYQFFCLWEKLPASMKRVAELVGIEEGFLARSIKGKITAKTEKQH----------------RQMAIHKRFFTSLALLDLISEVPLKDMTEKYGCSRGQLQSLQQSAATYAGMVTVFCNRLGWHNMELLLSQFQSRLTFGVHRELCDLVRVS--VLNAQRARTLYNAGFVTVADLAKASPDDVATALKNSVPFKS 788          

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|999977218|gb|KXJ16392.1| (DNA polymerase theta [Exaiptasia pallida])

HSP 1 Score: 362.844 bits (930), Expect = 1.914e-98
Identity = 215/607 (35.42%), Postives = 338/607 (55.68%), Query Frame = 0

Query:  566 TDAKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAG-----KRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAACLGP-----------WTEAVKV----QEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGE-GGGVERALLEVVM-GRLAGCKEEEGGEGWLETFARCTLYAVQAHEEEGKEENGEGLGVEGQACKVLQRFRRGLDYLVQNRYI 1150
            +D  NL S   WG+P  +++ Y ++G+  +F WQ  CL  G   VLR G NLVYSAPTS GKT+VAELLML+ +        ALF++P+V++A EK  Y +R++    + V+ F      A     V +AVCTIE+AN+L+NRL+E  +++ + VVVVDE+HM+GD  RG+L+E+ L+K+R  +               G        AE +++ G +T       N + +Q++ +SATLPNL  +A WL A LY TD RPV L+ ++  G     K++ ++R     +P+KA                   + ++   ++ LC E+++QGH VL+FC ++ W ++ A++IAR F+    P           + +A  +     E   E+  +L+ T VGL   L   + HGVA+HHAGLT++ER ++EG F+ G +  LVATSTL +GVNLPARRVI+R+   F G+ +    ++QM GRAGR G+D+ GE++L+ +  +   A       L P+ S L + E  GG++RALLEV+  G + G ++       +E +A CT +A  + +++           +G  C + +  +    +LV N +I
Sbjct:  271 SDVHNLAS---WGLPPSVLQRYHDMGITHMFEWQAECLRTGN--VLRGG-NLVYSAPTSAGKTMVAELLMLKRVLET--KRKALFILPYVSVAREKMFYLQRLFQEASVRVEGFMGSHNPAGGFTAVDIAVCTIEKANSLLNRLMEENKVSTLGVVVVDEMHMIGDPHRGYLLELFLTKIRFVS---------------GEGTVQGEQAECDNKDGKST-------NESPIQVVCMSATLPNLDMLARWLAADLYFTDHRPVPLTEMVKIGPTIYDKKMTKIRDI---DPTKA-------------------VVNDDDHIIPLCKETITQGHSVLIFCPTKNWCEKQAEAIARHFSTIGNPGLARKTDNRSGFVKASDMIAFDYEAIREVFEQLKRTPVGLDSVLRRVLPHGVAYHHAGLTVDERDIIEGAFRQGTIRVLVATSTLCSGVNLPARRVIIRTPT-FHGKMVDPLVYKQMAGRAGRKGVDDLGESVLVCKTTERHKATTLMKSELKPVQSCLLLKELNGGIKRALLEVIASGVVTGLRD-------VERYAACTFFASSSRQDDX----------DGNKCAITETIK----FLVDNEFI 803          

HSP 2 Score: 130.568 bits (327), Expect = 1.666e-26
Identity = 97/324 (29.94%), Postives = 145/324 (44.75%), Query Frame = 0

Query: 1166 VRDGETSGGSIVGPDESHLDPERGGGQGILQGANAAVDGTRPVPAATREHAGGAGPSLPTARPKTCYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTP--DWHDLARRSQRWAPEVASVAAAIGVSESAIERVVR---PRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPL-LQLETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAAE 1483
            +RD E           S  D   G    I +     VD         ++   G   S  +A+ +  Y  TQLG+AT  + +SP +A  +   L QA  K  +L S+LH+++   P       P  DW+      +R    +  VA  +G+ E  +    R   P  T  +R                   Q++ + RRF  +L L D++ EVPL +     G  RG +Q+LQS A  F GM+ VFC+ L WV L  L+     RL   V  +L  L R+   +++AARAR L+ AGFQ    +A+A+ E +  VL  + PF +E
Sbjct:  762 LRDVERYAACTFFASSSRQDDXDGNKCAITETIKFLVDNEFIRVQVNKDKENGDSRSEESAQER--YVSTQLGSATVASALSPDEALVVFGDLQQAR-KCFVLESELHIIYQVTPIYVEAQWPNLDWNQFLSIWERLPSHMRRVADIVGIQEVYLSLASRGRIPTRTPQQR-------------------QQLRVHRRFFAALALQDLVHEVPLNVVARRYGATRGMVQSLQSSASTFAGMVSVFCQKLGWVNLDLLLSQFQSRLSFGVERELCDLVRIS--VLNAARARMLYNAGFQTMASLASAKPEVIETVLRNAAPFVSE 1061          

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|89267985|emb|CAJ81454.1| (polymerase (DNA directed), theta, partial [Xenopus tropicalis])

HSP 1 Score: 347.436 bits (890), Expect = 2.039e-98
Identity = 220/596 (36.91%), Postives = 327/596 (54.87%), Query Frame = 0

Query:  572 LSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAACL------------GPWTEAVKV-QEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGEGGGVE----RALLEVVMGRLAGCKEEEGGEGWLETFARCTLYAVQAHEEEGKEENGEGLGVEGQACKVLQRFRRGLDYLVQNRYI 1150
            L    WG+P+ ++  Y  LGV ++F WQ  CL  GQ   + EG+NLVYSAPTS GKTLVAELL+L+ +        ALF++PFV++A+EK  Y + ++  V + V  +   +  A     + VAVCTIE+AN LVNRL+E  ++  + ++VVDELHM+GD+ RG+L+E++L+KVR                                Q+G           A  VQI+G+SATLPNL  +AAWLDA LY TDFRPV L         LER++       +   S   ++  +EPL  LP  +  +   ++ L  E+V  GH +L+FC S+ W ++ A +IAR F                  +   V + ++G  +++ +L+ +  GL   L  +V  GVAFHHAGLT +ER ++EG F+ G +  L ATSTL++GVNLPARRVI+RS + F+G+ + +  ++QM GRAGR G+D  GE+IL+ +  +     +     L P+ S L   EG GV     RA+LE+++G +A   E+      +  +A CTL A    +  G +E   G G   +AC         +D+L++N +I
Sbjct:  190 LMLASWGLPKTVLEKYGSLGVSQMFEWQAECLMLGQ---VLEGKNLVYSAPTSAGKTLVAELLILKRVLETRR--KALFILPFVSVAKEKTFYLQNLFQEVGVKVDGYMGSSSPAGGFCSLDVAVCTIEKANGLVNRLIEENKIDLLGMMVVDELHMLGDSHRGYLLELLLTKVRYVT-----------------------------QKGAVGRKAEGAGPANDVQIVGMSATLPNLGLLAAWLDAELYHTDFRPVPL---------LERVK----IGRTVYDSNMVAVREFEPL--LP--VKGDDDHIVSLSYETVWGGHSILIFCPSKNWCEKLADTIAREFYNLFHRAQQRGDAGGSDSFVPPVALDRDGIRDVMDQLKRSPAGLDAVLGRTVPWGVAFHHAGLTFDERDIVEGAFRQGFVRVLAATSTLSSGVNLPARRVIIRSPL-FNGRLLDILTYKQMAGRAGRKGVDTEGESILVCKNPERAKGISLLQGSLKPVQSCLLRKEGAGVTGSMIRAILEIIVGGVADTPED------VRIYASCTLLAASMKQGGGDQEEEHGGGAI-EAC---------VDWLLRNEFI 717          

HSP 2 Score: 73.9442 bits (180), Expect = 1.787e-9
Identity = 47/147 (31.97%), Postives = 76/147 (51.70%), Query Frame = 0

Query: 1231 CYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPLLQL 1377
             Y  T+LGAAT  + +SP +A  + A L +A  KG +L +DLH+L+L  P    + T DW+      ++    +  VA  +G+ E  + R V         NG +  +     ND+    +++++ RRF  SLVL D++ EV L +L
Sbjct:  731 VYRPTKLGAATLSSSLSPSEALGIFADLQRAM-KGFVLENDLHILYLVTPVYEEWTTIDWYQFFCLWEKLPTSMKRVAELVGIEEGFLARSV---------NGKIVAK-----NDRQ--HRQIAIHRRFFTSLVLLDLISEVSLKEL 860          

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|1126175383|ref|XP_019619912.1| (PREDICTED: DNA polymerase theta-like [Branchiostoma belcheri])

HSP 1 Score: 362.844 bits (930), Expect = 2.062e-98
Identity = 218/563 (38.72%), Postives = 314/563 (55.77%), Query Frame = 0

Query:  568 AKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKRLERLRRAPPPNPSKASSASASLSAWEPLRELPSTIS---DETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAAC------------LGPWTEAVKVQEGRN---ELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGEGGGV----ERALLEVVMGRLAGCKEEEGGEGWLETFARCTLYA 1108
            ++N L    WG+PE +++ Y +  + +LF WQ  CL+ G+   +  GRNLVYSAPTS GKT+VAELLML+ +    + G  +F++PFV++A EK  Y +++     + V  +      A     V VAVCTIE+AN+LVNRL+E G+  ++ +VVVDELHMVGD+ RG+L+E++L+K+R           + S + T N    +P                       VQI+G+SATLPNL  +A WLDA LY TDFRPV L+  +  G                    +   S  E +R+  S I+   DE Q V+ LC E+VS GH VLVFC ++ W ++ A++IAR F                   +E   VQ  R    +++ +LR T VGL   L   V +GVAFHHAGL+ +ER ++EG F+ G L  LVATSTL++GVNLPARRVI+R+ + F G+ +    ++QM GRAGR G+D  GE++L+ +E +   A       L P+ S LH   G  V    +RA+LE++ G +A    +      +E +A CT+ A
Sbjct:  135 SQNPLLLSSWGLPESVLQCYHDNRITQLFEWQAECLQTGE---VLAGRNLVYSAPTSAGKTMVAELLMLKRVLETQKKG--IFILPFVSVAREKMFYLKKLLFDAGVKVDGYMGGQSPAGGFSSVDVAVCTIEKANSLVNRLIEDGKTDQLGIVVVDELHMVGDSHRGYLLELLLTKIRY----------VTSKNRTDNKAATNP-----------------------VQIVGMSATLPNLDLLARWLDAELYQTDFRPVPLTECVKVG-------------------TTVYNSQMEKVRDFHSVINVRGDEDQ-VVGLCYETVSDGHSVLVFCPTKNWCEKLAETIAREFYNINRVPPEEKLTEGFSKLSELPYVQLNRQVLQDVVEQLRRTPVGLDHMLGRVVPYGVAFHHAGLSFDERDIVEGAFRQGHLRVLVATSTLSSGVNLPARRVIIRTPL-FHGRVLDSLTYKQMAGRAGRKGVDSQGESVLVCKERERSKATTLIRSGLSPVHSCLHRETGQDVSSSMKRAILEIIAGGVARSPAD------VERYASCTMLA 632          

HSP 2 Score: 128.257 bits (321), Expect = 7.499e-26
Identity = 97/312 (31.09%), Postives = 152/312 (48.72%), Query Frame = 0

Query: 1225 TARPKTCYFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPL-LQLETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAA--------EHDSAKPRQGPK---DGGRQGGGDKRVARRIADLIVRKAQEVARQ 1524
            T  P   Y  TQLG+AT  + +SP +A  + A L +A  K  +L ++LH+LF   P    +   DW+      ++    +  VA  +GV E  + R V  R                +   K +  +++++ +RF  +LVL D+ QEVPL + ++     +G LQ+LQ  A  F GM+ VFC  L W  L  L+     RL   V  +L+ L R+   L++  RAR LF AGF     +AAA V DV  VL  + PF +        E++ AK R+  +     G+ G  +   AR+    I+++A+E+ +Q
Sbjct:  673 TPSPSQEYQPTQLGSATLSSALSPDEALVVFADLQRAM-KSFVLENELHILFQVTPIYTDWPNLDWYQYLCLYEKLPTHMRRVAELVGVEEGYLARAVNGRVP--------------VKTPKQL--RQLAVHKRFYTALVLQDLTQEVPLPVVVKKYSCTKGVLQSLQQSAATFAGMVTVFCSRLGWHNLELLLAQFQSRLTFGVQRELVELVRIS--LLNGLRARTLFNAGFHTVAMLAAAEVRDVEVVLRNAAPFKSGRKADDETEYE-AKERETSRCFWVTGKAGLTEAEAARQ----IIQEAKELVQQ 960          

BLAST of EWM22669.1 vs. NCBI_GenBank
Match: gi|1025371780|ref|XP_016398017.1| (PREDICTED: DNA polymerase theta-like [Sinocyclocheilus rhinocerous])

HSP 1 Score: 362.073 bits (928), Expect = 3.099e-98
Identity = 219/583 (37.56%), Postives = 324/583 (55.57%), Query Frame = 0

Query:  545 GVDGSSHGGVPIPPGRASTEET---DAKNLLSYPGWGIPEVIVRSYEELGVRRLFPWQVACLEAGQGRVLREGRNLVYSAPTSGGKTLVAELLMLRALTHAVEGGTALFVVPFVALAEEKAGYFRRVWAGVDLGVKAFHSDAVDATLSEDVHVAVCTIERANALVNRLLERGELARVKVVVVDELHMVGDAGRGFLIEVMLSKVRLAASLWSQASLMASDHATGNAVRLDPAAEVNDQRGGATVDCTTRKNAARVQIIGLSATLPNLPQVAAWLDAHLYVTDFRPVQLSLLLCAGKR-----LERLRRAPPPNPSKASSASASLSAWEPLRELPSTISDETQAVLHLCLESVSQGHGVLVFCTSRAWTQRCAKSIARAFAACL------GPWTEAVKV-QEGRNELLARLRLTSVGLAKELEESVKHGVAFHHAGLTMEERTLLEGGFKTGVLSTLVATSTLAAGVNLPARRVIVRSRIGFDGQEMSVAQFQQMCGRAGRFGIDETGEAILMTREADVQAARAFAARCLPPMMSALHVGEGGGVE----RALLEVVMGRLAGCKEEEGGEGWLETFARCTLYA 1108
            G+D S  G   +P    S       D  + L    WG+P+  +  Y+ LGV+R+F WQ  CL  G  +VL EG+NLVYSAPTS GKTLV+ELL+L+ +    +   A+F++PFV++A EK  Y + V+  V + V+ +  +   A     + VAVCTIE+AN L+NRL+E  +L  + +VVVDELHMVGD+GRG+L+E++L+K++                           A+ N  RG A      +  +A VQI+G+SATLPNL  +A WL+A LY T++RPV L   +  G       +  +R+  P  P K                       +   ++ LC E+V  GH  L+FC S+ W ++ A SI R F          G   +++ + QEG  +++A+L+ T  GL + L  +V  GVAFHHAGLT +ER +LEG F+ G +  L ATSTL++GVNLPARRVI+R+ + F+G  + +  ++QM GRAGR G+D  GE++L+ +EA+     +     L P+ S L   EG GV     RA+LE+++G +A   ++      +  +A CTL A
Sbjct:  262 GLDLSVLGDATLPDAAQSIHMARPADQSDKLLLSNWGLPKPALEKYQSLGVQRMFEWQAECLTLG--KVL-EGQNLVYSAPTSAGKTLVSELLILKRVLETRQ--KAMFILPFVSVAREKMFYLQNVFQEVGVRVEGYMGNTSAAGGFSALDVAVCTIEKANGLINRLIEEDKLNLLGIVVVDELHMVGDSGRGYLLELLLTKIQY-------------------------IAQTNTARGSA------QSASAGVQIVGMSATLPNLELLARWLNAELYSTNYRPVPLMEWVKIGTNVYDGSMNLVRQFTPALPVKG----------------------DDDHIVGLCFETVQDGHSALLFCPSKNWCEKLADSIGREFYNLHHKEMQSGSGVQSISLNQEGLQDVVAQLKRTPAGLDQVLRRTVPWGVAFHHAGLTFDERDILEGAFRQGYIRVLAATSTLSSGVNLPARRVIIRTPV-FNGHLLDILTYKQMVGRAGRKGVDTMGESVLVCKEAERAKGMSLIQGSLKPISSCLVKREGEGVTTSMLRAILEIIVGGVASSPQD------VRMYASCTLLA 779          

HSP 2 Score: 122.479 bits (306), Expect = 4.735e-24
Identity = 79/252 (31.35%), Postives = 126/252 (50.00%), Query Frame = 0

Query: 1232 YFGTQLGAATFFAGMSPRDATAMLASLSQATTKGVILSSDLHLLFLCVPPNRRYFTPDWHDLARRSQRWAPEVASVAAAIGVSESAIERVVRPRSTGGRRNGGLGERGDGLGNDKSVGGQEVSLLRRFVYSLVLHDMLQEVPLLQL-ETGGVGRGTLQALQSEARIFCGMIVVFCKHLQWVTLARLIEGLSLRLEHRVGEDLLPLCRMGPELVHAARARALFEAGFQAPEDVAAARVEDVAAVLLTSMPFAA 1482
            Y  T LG+AT  + +SP +A  + A L +A  KG +L +DLH+L+   P    + T DW+      ++    +  VA  +G+ E  +      RS GG+           L        +++++ +RF  +LVLH+++ E PL  + +  G  RG LQ+LQ  A  + GM+ VFC  L W  L  L+     RL   V  +L  L R+   L++A RAR L+ +GF    +VA A V ++   L  ++PF +
Sbjct:  826 YCPTHLGSATLSSSLSPPEALGIFADLQRAM-KGFVLENDLHILYQITPVYVDWTTIDWYQFFCLWEQLPSAMKRVAEMVGIQEGFL-----ARSVGGK-----------LIAKTEKQCRQMAIHKRFFTTLVLHELVSEEPLGAVAKKYGCSRGQLQSLQQSASTYAGMVTVFCNRLGWHNLELLLSQFQSRLSFGVQRELCDLVRIS--LLNAQRARTLYSSGFVTVAEVARADVTELEKALRKAIPFKS 1058