EWM23049.1, cds7622 (CDS) Nannochloropsis gaditana

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|585103859|gb|EWM23049.1| (medium-chain-fatty-acid- ligase (medium-chain acyl- synthetase) [Nannochloropsis gaditana])

HSP 1 Score: 576.63 bits (1485), Expect = 0.000e+0
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0

Query:    1 MHGRSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGVPPEEGERDGGVRAGHQESFYTDDDGERWFLTGDVATICPDGYIKITDR 280
            MHGRSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGVPPEEGERDGGVRAGHQESFYTDDDGERWFLTGDVATICPDGYIKITDR
Sbjct:    1 MHGRSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGVPPEEGERDGGVRAGHQESFYTDDDGERWFLTGDVATICPDGYIKITDR 280          

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|553194324|ref|XP_005855929.1| (acyl-CoA synthetase [Nannochloropsis gaditana CCMP526] >gi|422293130|gb|EKU20430.1| acyl-CoA synthetase [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 356.681 bits (914), Expect = 4.388e-118
Identity = 185/232 (79.74%), Postives = 185/232 (79.74%), Query Frame = 0

Query:    1 MHGRSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 232
            MHGRSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIV HAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTG                                              IPYTAAMVGCKLVLPGSALDGASLYELMKEEGV
Sbjct:    1 MHGRSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVHHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTG----------------------------------------------IPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 186          

HSP 2 Score: 106.301 bits (264), Expect = 2.042e-22
Identity = 47/47 (100.00%), Postives = 47/47 (100.00%), Query Frame = 0

Query:  234 PEEGERDGGVRAGHQESFYTDDDGERWFLTGDVATICPDGYIKITDR 280
            PEEGERDGGVRAGHQESFYTDDDGERWFLTGDVATICPDGYIKITDR
Sbjct:  323 PEEGERDGGVRAGHQESFYTDDDGERWFLTGDVATICPDGYIKITDR 369          

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|1012141896|gb|KYP12566.1| (long-chain fatty acid--CoA ligase [Limnobacter sp. CACIAM 66H1])

HSP 1 Score: 292.352 bits (747), Expect = 2.084e-92
Identity = 134/229 (58.52%), Postives = 174/229 (75.98%), Query Frame = 0

Query:    4 RSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 232
            R+KKL N L ELGV++G+ +ATLA N YRH+E+Y+AVSG GAV+HTLNPRLF E L +I+ HA+D+++FFD  FA L+  + P CP+VK W+ + DE  MP  P+++         NYEAL++   + YVWP L+E  AS LCYTSGTTG PKG LYSHRSTVLH++AA  PD+L +S+RDT+LP VP+FHVNAWGIPY A M G K+V+PG ALDGAS++EL++ E V
Sbjct:   48 RAKKLANTLTELGVQQGENIATLAWNGYRHVEIYYAVSGMGAVVHTLNPRLFPEQLVYIINHAKDTMVFFDSTFAPLIRAIAPACPTVKRWVQMCDEAVMPSEPAVA------GCLNYEALIKNASDQYVWPQLDENTASGLCYTSGTTGNPKGALYSHRSTVLHAMAACTPDALGLSSRDTILPVVPMFHVNAWGIPYIALMTGAKMVMPGGALDGASIFELLESEKV 270          

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|149824834|gb|EDM84048.1| (acyl-CoA synthase [Limnobacter sp. MED105])

HSP 1 Score: 292.352 bits (747), Expect = 2.261e-92
Identity = 134/229 (58.52%), Postives = 175/229 (76.42%), Query Frame = 0

Query:    4 RSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 232
            R+KKL N L  LGV++G+ +ATLA N YRH+E+Y+AVSG GAV+HTLNPRLF E L +I+ HA+D+++FFD  FA L++ + P CP+VK W+ + DE  MP   +++         NYEAL+++  + YVWP L+E AAS LCYTSGTTG PKG LYSHRSTVLH++AA  PD+L +S+RDT+LP VP+FHVNAWGIPY A M G K+V+PG ALDGAS+YEL++ E V
Sbjct:   43 RTKKLANTLTSLGVQQGENIATLAWNGYRHVEIYYAVSGMGAVVHTLNPRLFPEQLVYIINHAKDTMVFFDSTFAPLIKAIAPACPTVKRWVQMCDEAAMPAEQAVA------GCLNYEALIKDASDHYVWPQLDENAASGLCYTSGTTGNPKGALYSHRSTVLHAMAACTPDALGLSSRDTILPVVPMFHVNAWGIPYIALMTGAKMVMPGGALDGASIYELLESEKV 265          

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|1180278312|ref|WP_083797117.1| (long-chain fatty acid--CoA ligase [Limnobacter sp. MED105])

HSP 1 Score: 291.967 bits (746), Expect = 2.945e-92
Identity = 134/229 (58.52%), Postives = 175/229 (76.42%), Query Frame = 0

Query:    4 RSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 232
            R+KKL N L  LGV++G+ +ATLA N YRH+E+Y+AVSG GAV+HTLNPRLF E L +I+ HA+D+++FFD  FA L++ + P CP+VK W+ + DE  MP   +++         NYEAL+++  + YVWP L+E AAS LCYTSGTTG PKG LYSHRSTVLH++AA  PD+L +S+RDT+LP VP+FHVNAWGIPY A M G K+V+PG ALDGAS+YEL++ E V
Sbjct:   44 RTKKLANTLTSLGVQQGENIATLAWNGYRHVEIYYAVSGMGAVVHTLNPRLFPEQLVYIINHAKDTMVFFDSTFAPLIKAIAPACPTVKRWVQMCDEAAMPAEQAVA------GCLNYEALIKDASDHYVWPQLDENAASGLCYTSGTTGNPKGALYSHRSTVLHAMAACTPDALGLSSRDTILPVVPMFHVNAWGIPYIALMTGAKMVMPGGALDGASIYELLESEKV 266          

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|731478114|gb|AIZ64422.1| (long-chain fatty acid--CoA ligase [Hymenobacter sp. DG25B])

HSP 1 Score: 288.886 bits (738), Expect = 5.766e-91
Identity = 139/231 (60.17%), Postives = 166/231 (71.86%), Query Frame = 0

Query:    2 HGRSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 232
            H RSK+L N L+ELG+  G+RV TLA NT+RH ELY+ +SG GAV HT+NPRLF E L +I+ HA+D ++FFD  F  LVERL P CP+V+ W+ + D   MP   +L        +  YE LL    E++ WP+ +E  ASSLCYTSGTT  PKGVLYSHRSTVLHS AAALPD  N SARD +LP VP+FHVNAWGIPY A M+GCKLVLPG  LD ASLYEL + EGV
Sbjct:   46 HRRSKQLANALKELGINWGERVGTLAWNTHRHFELYYGISGMGAVCHTINPRLFPEQLVYIINHAEDRLIFFDLTFLPLVERLAPLCPTVEAWVLMTDRAHMPEATALPGE-----VRCYEDLLAAHNEEFTWPVFDENTASSLCYTSGTTDQPKGVLYSHRSTVLHSYAAALPDCFNCSARDVILPVVPMFHVNAWGIPYLAPMIGCKLVLPGPGLDAASLYELFENEGV 271          

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|1175474303|ref|WP_082017110.1| (long-chain fatty acid--CoA ligase [Hymenobacter sp. DG25B])

HSP 1 Score: 288.5 bits (737), Expect = 5.900e-91
Identity = 139/231 (60.17%), Postives = 166/231 (71.86%), Query Frame = 0

Query:    2 HGRSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 232
            H RSK+L N L+ELG+  G+RV TLA NT+RH ELY+ +SG GAV HT+NPRLF E L +I+ HA+D ++FFD  F  LVERL P CP+V+ W+ + D   MP   +L        +  YE LL    E++ WP+ +E  ASSLCYTSGTT  PKGVLYSHRSTVLHS AAALPD  N SARD +LP VP+FHVNAWGIPY A M+GCKLVLPG  LD ASLYEL + EGV
Sbjct:   42 HRRSKQLANALKELGINWGERVGTLAWNTHRHFELYYGISGMGAVCHTINPRLFPEQLVYIINHAEDRLIFFDLTFLPLVERLAPLCPTVEAWVLMTDRAHMPEATALPGE-----VRCYEDLLAAHNEEFTWPVFDENTASSLCYTSGTTDQPKGVLYSHRSTVLHSYAAALPDCFNCSARDVILPVVPMFHVNAWGIPYLAPMIGCKLVLPGPGLDAASLYELFENEGV 267          

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|586954894|gb|AHJ96628.1| (AMP-dependent synthetase and ligase [Hymenobacter swuensis DY53])

HSP 1 Score: 288.115 bits (736), Expect = 8.706e-91
Identity = 139/229 (60.70%), Postives = 168/229 (73.36%), Query Frame = 0

Query:    4 RSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 232
            RS++L N L+ LG++ GDRV TLA NT+RH+ELY+AVSG+GAV HT NPRLF E L +I+ HA+D +LFFD  F  LVE+L P CP+V+ W+ + D   MP   +L       +L  YE LL    +++ WP+ +E  ASSLCYTSGTT  PKGVLYSHRST+LHS AAALPD  N SARD +LP VP+FHVNAWGIPY A MVGCKLVLPG  LD ASLYEL + EGV
Sbjct:   44 RSRQLANALQRLGIRSGDRVGTLAWNTHRHLELYYAVSGSGAVCHTSNPRLFPEQLVYIINHAEDRLLFFDLTFLPLVEKLAPSCPTVEAWVLMTDRAHMPETTTLP------NLRCYEDLLAAESDEFEWPVFDENTASSLCYTSGTTDQPKGVLYSHRSTLLHSYAAALPDCFNCSARDVILPVVPMFHVNAWGIPYLAPMVGCKLVLPGPGLDAASLYELFETEGV 266          

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|1175220421|ref|WP_081768283.1| (long-chain fatty acid--CoA ligase [Hymenobacter swuensis])

HSP 1 Score: 288.115 bits (736), Expect = 1.096e-90
Identity = 139/229 (60.70%), Postives = 168/229 (73.36%), Query Frame = 0

Query:    4 RSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 232
            RS++L N L+ LG++ GDRV TLA NT+RH+ELY+AVSG+GAV HT NPRLF E L +I+ HA+D +LFFD  F  LVE+L P CP+V+ W+ + D   MP   +L       +L  YE LL    +++ WP+ +E  ASSLCYTSGTT  PKGVLYSHRST+LHS AAALPD  N SARD +LP VP+FHVNAWGIPY A MVGCKLVLPG  LD ASLYEL + EGV
Sbjct:   49 RSRQLANALQRLGIRSGDRVGTLAWNTHRHLELYYAVSGSGAVCHTSNPRLFPEQLVYIINHAEDRLLFFDLTFLPLVEKLAPSCPTVEAWVLMTDRAHMPETTTLP------NLRCYEDLLAAESDEFEWPVFDENTASSLCYTSGTTDQPKGVLYSHRSTLLHSYAAALPDCFNCSARDVILPVVPMFHVNAWGIPYLAPMVGCKLVLPGPGLDAASLYELFETEGV 271          

BLAST of EWM23049.1 vs. NCBI_GenBank
Match: gi|1209960518|ref|WP_088464313.1| (long-chain fatty acid--CoA ligase [Hymenobacter sp. CCM 8682] >gi|1209171467|gb|OWP63176.1| long-chain fatty acid--CoA ligase [Hymenobacter sp. CCM 8682])

HSP 1 Score: 287.73 bits (735), Expect = 1.375e-90
Identity = 137/229 (59.83%), Postives = 166/229 (72.49%), Query Frame = 0

Query:    4 RSKKLGNILEELGVKKGDRVATLAMNTYRHMELYFAVSGAGAVLHTLNPRLFAETLTWIVRHAQDSVLFFDPCFASLVERLLPHCPSVKHWICLVDEERMPVLPSLSPSSPFLSLHNYEALLREGKEDYVWPILEETAASSLCYTSGTTGPPKGVLYSHRSTVLHSLAAALPDSLNVSARDTVLPAVPLFHVNAWGIPYTAAMVGCKLVLPGSALDGASLYELMKEEGV 232
            RS++L N L  LG+  GDRV TLA NT+RH+ELY+AVSG+GAV HT+NPRLF E + +I+ HAQD  +FFD  F  LVE+L  HCP V+ W+ + D   MP       S+   +LH YE L+     DY WP+ +E  ASSLCYTSGTT  PKGVLYSHRST+LHS AAALPD  N S++D +LP VP+FHVNAWGIPY A M+GCKLVLPG  LD ASLYEL ++EGV
Sbjct:   44 RSRQLANALARLGIANGDRVGTLAWNTHRHLELYYAVSGSGAVCHTINPRLFPEQIVYIIGHAQDRAVFFDLTFLPLVEKLAAHCPGVEAWVLMSDRAHMP------ESTALPNLHCYEDLVAAENADYQWPVFDENTASSLCYTSGTTAEPKGVLYSHRSTLLHSFAAALPDCFNCSSQDVILPVVPMFHVNAWGIPYLAPMLGCKLVLPGPGLDAASLYELFEQEGV 266