EWM23097.1, cds7577 (CDS) Nannochloropsis gaditana

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|585103959|gb|EWM23097.1| (protease do-like 2 [Nannochloropsis gaditana])

HSP 1 Score: 1358.58 bits (3515), Expect = 0.000e+0
Identity = 657/657 (100.00%), Postives = 657/657 (100.00%), Query Frame = 0

Query:    1 MSARSIRENSLTAAKMGGLAALLMMLLVAVAPPPGLAFFQPAGLISSRLQSIAPPPPTSPPNACPGTKTSYFLPSFPSLTVFGAIKGRATRTSLYAAAPASPASSSSGNAGRDEDEEGSFPDESDSPTPSSPDTWRSNGGEALPPTKHTTQIDLTGIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAWAFDVKEKLLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNGEYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVAGNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPSRPQKQSFLMVGGLLFLPLSWEILDPDRSTRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDARENAVWNATQEICEIYSVPYYKPLPARKEGEEGGESELPLWQRSHAKGEGTKW 657
            MSARSIRENSLTAAKMGGLAALLMMLLVAVAPPPGLAFFQPAGLISSRLQSIAPPPPTSPPNACPGTKTSYFLPSFPSLTVFGAIKGRATRTSLYAAAPASPASSSSGNAGRDEDEEGSFPDESDSPTPSSPDTWRSNGGEALPPTKHTTQIDLTGIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAWAFDVKEKLLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNGEYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVAGNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPSRPQKQSFLMVGGLLFLPLSWEILDPDRSTRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDARENAVWNATQEICEIYSVPYYKPLPARKEGEEGGESELPLWQRSHAKGEGTKW
Sbjct:    1 MSARSIRENSLTAAKMGGLAALLMMLLVAVAPPPGLAFFQPAGLISSRLQSIAPPPPTSPPNACPGTKTSYFLPSFPSLTVFGAIKGRATRTSLYAAAPASPASSSSGNAGRDEDEEGSFPDESDSPTPSSPDTWRSNGGEALPPTKHTTQIDLTGIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAWAFDVKEKLLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNGEYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVAGNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPSRPQKQSFLMVGGLLFLPLSWEILDPDRSTRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDARENAVWNATQEICEIYSVPYYKPLPARKEGEEGGESELPLWQRSHAKGEGTKW 657          

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|553194401|ref|XP_005855948.1| (protease do-like 2 [Nannochloropsis gaditana CCMP526] >gi|422293121|gb|EKU20421.1| protease do-like 2 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 380.948 bits (977), Expect = 2.480e-126
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0

Query:  259 MEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNGEYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVAGNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMS 440
            MEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNGEYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVAGNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMS
Sbjct:    1 MEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNGEYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVAGNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMS 182          

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|545703477|ref|XP_005704064.1| (serine-type endopeptidase isoform 1 [Galdieria sulphuraria] >gi|452820503|gb|EME27544.1| serine-type endopeptidase isoform 1 [Galdieria sulphuraria])

HSP 1 Score: 297.745 bits (761), Expect = 4.273e-89
Identity = 182/476 (38.24%), Postives = 270/476 (56.72%), Query Frame = 0

Query:  156 GIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAWAFDVKEKLLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDV-QLGELVTVVGYPRGGEKICLTKGIVSRL------HFNGEYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRES--VAGNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPSRPQKQSFLMVGGLLFLPLSWEIL--------DPDRSTRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDARENAVWNATQ 614
            G P  V+ S+FKL VT C P +  PW  Q    ++ST+ AF V  + +LTN+HCV  + V+ +KK G+   + A+V+   +  D+AL+ V DE FWEGVE   + +G +  L E VTVVGYP GGE I +T G+VSR+      H   + L +QIDAAINPGNSGGP  N R +CVGIA++  +   +ENIGYIIP  VV HFL+DF R+G   G C  GFE Q + N  LR+S  ++    G L+  +A  +  +  LQKGDV+   DG  + ND TV ++  + INF +L++  FVG+   V ++R   ++E     + P           +E+    PI   RP  + +L V GL+F+ LS   L        D +   + L       +R +A+E+VV+LS++    IN GY     T +L FN   V NL HL  +I++ +E+++RF  D    ++IV++ ++ A+  ++Q
Sbjct:  100 GFPADVLDSVFKLYVTHCEPNYSLPW--QKRRQTYSTSTAFAVGNRRILTNAHCVEHSTVVKIKKRGSEKKYMAQVVSIGNDCDIALLSVEDESFWEGVEC--LSSGRLPYLQEAVTVVGYPIGGENISVTAGVVSRVELQQYSHGAIDLLGVQIDAAINPGNSGGPAFNSRFECVGIAFQSLLTTEAENIGYIIPWLVVQHFLDDFDRNGYYTGFCYCGFEFQRMENEYLRKSFHLSDESGGVLIKRIAPTSPCSKVLQKGDVITHFDGVPIANDGTVSYRGGERINFHYLITLKFVGESCTVRIVRNGNIME----VSYP----------LFELPLLVPIHEKRPVPE-YLTVAGLVFVALSEPYLRSEYGEKWDFEAPVKLLDKLLYGYKR-TANEQVVILSQVLNARINVGYECLTNTELLHFNGTRVENLCHLANLIDETSEEFLRF--DLEFDEVIVIE-KQAALEQSSQ 552          

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|545703475|ref|XP_005704063.1| (serine-type endopeptidase isoform 2 [Galdieria sulphuraria] >gi|452820502|gb|EME27543.1| serine-type endopeptidase isoform 2 [Galdieria sulphuraria])

HSP 1 Score: 291.197 bits (744), Expect = 2.227e-86
Identity = 182/491 (37.07%), Postives = 270/491 (54.99%), Query Frame = 0

Query:  156 GIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAWAFDVKEKLLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDV-QLGELVTVVGYPRGGEKICLTKGIVSRL------HFNGEYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESV-------------AGNH----TGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPSRPQKQSFLMVGGLLFLPLSWEIL--------DPDRSTRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDARENAVWNATQ 614
            G P  V+ S+FKL VT C P +  PW  Q    ++ST+ AF V  + +LTN+HCV  + V+ +KK G+   + A+V+   +  D+AL+ V DE FWEGVE   + +G +  L E VTVVGYP GGE I +T G+VSR+      H   + L +QIDAAINPGNSGGP  N R +CVGIA++  +   +ENIGYIIP  VV HFL+DF R+G   G C  GFE Q + N  LR+S               G H       ++ ++A  +  +  LQKGDV+   DG  + ND TV ++  + INF +L++  FVG+   V ++R   ++E     + P           +E+    PI   RP  + +L V GL+F+ LS   L        D +   + L       +R +A+E+VV+LS++    IN GY     T +L FN   V NL HL  +I++ +E+++RF  D    ++IV++ ++ A+  ++Q
Sbjct:  100 GFPADVLDSVFKLYVTHCEPNYSLPW--QKRRQTYSTSTAFAVGNRRILTNAHCVEHSTVVKIKKRGSEKKYMAQVVSIGNDCDIALLSVEDESFWEGVEC--LSSGRLPYLQEAVTVVGYPIGGENISVTAGVVSRVELQQYSHGAIDLLGVQIDAAINPGNSGGPAFNSRFECVGIAFQSLLTTEAENIGYIIPWLVVQHFLDDFDRNGYYTGFCYCGFEFQRMENEYLRKSFHLSDESGGVLIKRVGEHEIFFMKLIIAQIAPTSPCSKVLQKGDVITHFDGVPIANDGTVSYRGGERINFHYLITLKFVGESCTVRIVRNGNIME----VSYP----------LFELPLLVPIHEKRPVPE-YLTVAGLVFVALSEPYLRSEYGEKWDFEAPVKLLDKLLYGYKR-TANEQVVILSQVLNARINVGYECLTNTELLHFNGTRVENLCHLANLIDETSEEFLRF--DLEFDEVIVIE-KQAALEQSSQ 567          

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|1085187585|ref|XP_002370490.2| (trypsin domain-containing protein [Toxoplasma gondii ME49] >gi|527308735|gb|EPT25374.1| trypsin domain-containing protein [Toxoplasma gondii ME49])

HSP 1 Score: 282.722 bits (722), Expect = 1.284e-81
Identity = 179/473 (37.84%), Postives = 260/473 (54.97%), Query Frame = 0

Query:  155 TGIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAW-AFDVKEK-LLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNG------EYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVA--GNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPS-----RPQKQSFLMVGGLLFLPLSWEILDPDRS--------TRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDA 604
            T +  +V  ++FK+  T C P + +PW+ +  +TS ST +   D   K  LLTN+H V  A V+ V+K G+   ++AEVL      DLA++RV D  FW+G+          QLG+ VTVVGYP GG+   +T+G+VSR             LAIQIDAAINPGNSGGP LN    CVGIA++   D  +ENIGYIIP EVV HFLED+RRHG+ +G    GF  Q L N +LR S++      G L+ ++     A A LQKGD++L I G ++ +D TV F++ + I F +++S  FVGD   V++LR+              + +  KLNL         ++P+     RPQ   +L+VGGL+F+PLS   L  +           R L  +    +     E+ V+LS +  H +  GY      +V  FN   V+ L HL  ++E+ TE++ RF +D +  +++VL+A
Sbjct:  275 TILKSEVPNTVFKVFCTHCEPNYSQPWTTRRQTTSMSTGFVTLDANGKQCLLTNAHSVEHAAVVQVRKRGDHQKYEAEVLCIGLECDLAMLRVSDADFWKGLGPPLQWGPSPQLGDPVTVVGYPLGGDNSSVTQGVVSRADLQQYCLGSCSLLAIQIDAAINPGNSGGPALNRSSQCVGIAFQSLKDGDTENIGYIIPSEVVSHFLEDYRRHGRCLGFGDGGFTWQKLENKSLRHSLSLKSKDEGILIKKLDGGGPAKAVLQKGDILLEIGGKRIASDGTVAFRNGERILFTWILSQMFVGDRCSVKLLRQN--------RERRESFSVGKLNL---------LVPANSDLRRPQ---YLIVGGLVFVPLSEPFLKSEYGEDFESRAPVRLLDKWQHGFQSFPG-EQFVLLSHVLAHDVTVGYEHLHNVQVQQFNGTSVKTLKHLAELVENSTEEYWRFDLDHD--EVVVLEA 724          

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|672569983|gb|KFH09397.1| (trypsin domain-containing protein [Toxoplasma gondii VAND])

HSP 1 Score: 282.722 bits (722), Expect = 1.353e-81
Identity = 179/473 (37.84%), Postives = 260/473 (54.97%), Query Frame = 0

Query:  155 TGIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAW-AFDVKEK-LLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNG------EYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVA--GNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPS-----RPQKQSFLMVGGLLFLPLSWEILDPDRS--------TRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDA 604
            T +  +V  ++FK+  T C P + +PW+ +  +TS ST +   D   K  LLTN+H V  A V+ V+K G+   ++AEVL      DLA++RV D  FW+G+          QLG+ VTVVGYP GG+   +T+G+VSR             LAIQIDAAINPGNSGGP LN    CVGIA++   D  +ENIGYIIP EVV HFLED+RRHG+ +G    GF  Q L N +LR S++      G L+ ++     A A LQKGD++L I G ++ +D TV F++ + I F +++S  FVGD   V++LR+              + +  KLNL         ++P+     RPQ   +L+VGGL+F+PLS   L  +           R L  +    +     E+ V+LS +  H +  GY      +V  FN   V+ L HL  ++E+ TE++ RF +D +  +++VL+A
Sbjct:  275 TILKSEVPNTVFKVFCTHCEPNYSQPWTTRRQTTSMSTGFVTLDANGKQCLLTNAHSVEHAAVVQVRKRGDHQKYEAEVLCIGLECDLAMLRVSDADFWKGLGPPLQWGPSPQLGDPVTVVGYPLGGDNSSVTQGVVSRADLQQYCLGSCSLLAIQIDAAINPGNSGGPALNRSSQCVGIAFQSLKDGDTENIGYIIPSEVVSHFLEDYRRHGRCLGFGDGGFTWQKLENKSLRHSLSLKSKDEGILIKKLDGGGPAKAVLQKGDILLEIGGKRIASDGTVAFRNGERILFTWILSQMFVGDRCSVKLLRQN--------RERRESFSVGKLNL---------LVPANSDLRRPQ---YLIVGGLVFVPLSEPFLKSEYGEDFESRAPVRLLDKWQHGFQSFPG-EQFVLLSHVLAHDVTVGYEHLHNVQVQQFNGTSVKTLKHLAELVENSTEEYWRFDLDHD--EVVVLEA 724          

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|557731707|gb|ESS28089.1| (trypsin domain-containing protein [Toxoplasma gondii VEG] >gi|820703087|tpe|CEL78822.1| TPA: trypsin, putative [Toxoplasma gondii VEG])

HSP 1 Score: 282.337 bits (721), Expect = 1.635e-81
Identity = 179/473 (37.84%), Postives = 260/473 (54.97%), Query Frame = 0

Query:  155 TGIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAW-AFDVKEK-LLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNG------EYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVA--GNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPS-----RPQKQSFLMVGGLLFLPLSWEILDPDRS--------TRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDA 604
            T +  +V  ++FK+  T C P + +PW+ +  +TS ST +   D   K  LLTN+H V  A V+ V+K G+   ++AEVL      DLA++RV D  FW+G+          QLG+ VTVVGYP GG+   +T+G+VSR             LAIQIDAAINPGNSGGP LN    CVGIA++   D  +ENIGYIIP EVV HFLED+RRHG+ +G    GF  Q L N +LR S++      G L+ ++     A A LQKGD++L I G ++ +D TV F++ + I F +++S  FVGD   V++LR+              + +  KLNL         ++P+     RPQ   +L+VGGL+F+PLS   L  +           R L  +    +     E+ V+LS +  H +  GY      +V  FN   V+ L HL  ++E+ TE++ RF +D +  +++VL+A
Sbjct:  268 TILKSEVPNTVFKVFCTHCEPNYSQPWTTRRQTTSMSTGFVTLDANGKQCLLTNAHSVEHAAVVQVRKRGDHQKYEAEVLCIGLECDLAMLRVSDADFWKGLGPPLQWGPSPQLGDPVTVVGYPLGGDNSSVTQGVVSRADLQQYCLGSCSLLAIQIDAAINPGNSGGPALNRSSQCVGIAFQSLKDGDTENIGYIIPSEVVSHFLEDYRRHGRCLGFGDGGFTWQKLENKSLRHSLSLKSKDEGILIKKLDGGGPAKAVLQKGDILLEIGGKRIASDGTVAFRNGERILFTWILSQMFVGDRCSVKLLRQN--------RERRESFSVGKLNL---------LVPANSDLRRPQ---YLIVGGLVFVPLSEPFLKSEYGEDFESRAPVRLLDKWQHGFQSFPG-EQFVLLSHVLAHDVTVGYEHLHNVQVQQFNGTSVKTLKHLAELVENSTEEYWRFDLDHD--EVVVLEA 717          

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|523576571|gb|EPR63244.1| (trypsin domain-containing protein [Toxoplasma gondii GT1] >gi|672259556|gb|KFG34324.1| trypsin domain-containing protein [Toxoplasma gondii GAB2-2007-GAL-DOM2])

HSP 1 Score: 281.567 bits (719), Expect = 3.143e-81
Identity = 179/473 (37.84%), Postives = 259/473 (54.76%), Query Frame = 0

Query:  155 TGIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAW-AFDVKEK-LLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNG------EYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESVA--GNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPS-----RPQKQSFLMVGGLLFLPLSWEILDPDRS--------TRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDA 604
            T +  +V  ++FK+  T C P + +PW+ +  +TS ST +   D   K  LLTN+H V  A V+ V+K G+   ++AEVL      DLA++RV D  FW+G+          QLG+ VTVVGYP GG+   +T+G+VSR             LAIQIDAAINPGNSGGP LN    CVGIA++   D  +ENIGYIIP EVV HFLED+RRHG+ +G    GF  Q L N +LR S++      G L+ ++     A A LQKGD++L I G ++ +D TV F++ + I F +++S  FVGD   V++LR+              + +  KLNL         ++P+     RPQ   +L+VGGL+F+PLS   L  +           R L  +    +     E+ V+LS +  H +  GY      +V  FN   V+ L HL  ++E+ TE + RF +D +  +++VL+A
Sbjct:  268 TILKSEVPNTVFKVFCTHCEPNYSQPWTTRRQTTSMSTGFVTLDANGKQCLLTNAHSVEHAAVVQVRKRGDHQKYEAEVLCIGLECDLAMLRVSDADFWKGLGPPLQWGPSPQLGDPVTVVGYPLGGDNSSVTQGVVSRADLQQYCLGSCSLLAIQIDAAINPGNSGGPALNRSSQCVGIAFQSLKDGDTENIGYIIPSEVVSHFLEDYRRHGRCLGFGDGGFTWQKLENKSLRHSLSLKSKDEGILIKKLDGGGPAKAVLQKGDILLEIGGKRIASDGTVAFRNGERILFTWILSQMFVGDRCSVKLLRQN--------RERRESFSVGKLNL---------LVPANSDLRRPQ---YLIVGGLVFVPLSEPFLKSEYGEDFESRAPVRLLDKWQHGFQSFPG-EQFVLLSHVLAHDVTVGYEHLHNVQVQQFNGTSVKTLKHLAELVENSTEKYWRFDLDHD--EVVVLEA 717          

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|675130346|ref|XP_008887060.1| (trypsin domain-containing protein [Hammondia hammondi] >gi|661334072|gb|KEP62220.1| trypsin domain-containing protein [Hammondia hammondi])

HSP 1 Score: 281.182 bits (718), Expect = 5.004e-81
Identity = 179/473 (37.84%), Postives = 260/473 (54.97%), Query Frame = 0

Query:  155 TGIPGQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAW-AFDVK-EKLLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDVQLGELVTVVGYPRGGEKICLTKGIVSRLHFNG------EYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRE--SVAGNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLTWEMQKRHPILPS-----RPQKQSFLMVGGLLFLPLSWEILDPDRS--------TRALRSFCESVRRISADEEVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDA 604
            T +  +V  ++FK+  T C P + +PW+ +  +TS ST +   D   +K LLTN+H V  A V+ V+K G+   ++AEVL      DLA++RV D  FW+G+          QLG+ VTVVGYP GG+   +T+G+VSR             LAIQIDAAINPGNSGGP LN    CVGIA++   D  +ENIGYIIP EVV HFLED+RRHG+ +G    GF  Q L N +LR   S+     G L+ ++     A A L+KGD++L I G K+ +D TV F++ + I F +++S  FVGD   V++LR+              + +  KLNL         ++P+     RPQ   +L+VGGL+F+PLS   L  +           R L  +    +     E+ V+LS +  H +  GY      +V  FN   V+ L HL  ++E+ TE++ RF +D +  +++VL+A
Sbjct:  271 TILKSEVPNTVFKVFCTHCEPNYSQPWTTRRQTTSMSTGFVTLDANGKKCLLTNAHSVEHAAVVQVRKRGDHQKYEAEVLCIGLECDLAMLRVSDPDFWKGLGPPLQWGPSPQLGDPVTVVGYPLGGDNSSVTQGVVSRADLQQYCLGSCSLLAIQIDAAINPGNSGGPALNRSSQCVGIAFQSLKDGDTENIGYIIPSEVVSHFLEDYRRHGRCLGFGDGGFTWQKLENKSLRHALSLKSKDEGILIKKLDGGGPAKAVLRKGDILLEIGGKKIASDGTVAFRNGERILFTWILSQMFVGDRCSVKLLRQN--------RERRESFSVGKLNL---------LVPANSDLRRPQ---YLIVGGLVFVPLSEPFLKSEYGEDFESRAPVRLLDKWQHGFQSFPG-EQFVLLSHVLAHDVTVGYEHLHNVQVQHFNGTSVKTLKHLAELVENSTEEYWRFDLDHD--EVVVLEA 720          

BLAST of EWM23097.1 vs. NCBI_GenBank
Match: gi|697158058|ref|XP_009587776.1| (PREDICTED: protease Do-like 2, chloroplastic isoform X1 [Nicotiana tomentosiformis])

HSP 1 Score: 277.715 bits (709), Expect = 7.677e-81
Identity = 177/533 (33.21%), Postives = 292/533 (54.78%), Query Frame = 0

Query:  112 RDEDEEGSFPDESDSPTP-----SSPDTWRSNG----GEALPPTKHTTQIDLTGIP-GQVMGSIFKLKVTSCPPLFRRPWSRQPTSTSFSTAWAFDVKEKLLLTNSHCVADAVVINVKKPGNADLFKAEVLGKSDHSDLALIRVPDEVFWEGVEAIRMEAGDV-QLGELVTVVGYPRGGEKICLTKGIVSRL------HFNGEYLAIQIDAAINPGNSGGPVLNERGDCVGIAYRKRVDRGSENIGYIIPVEVVHHFLEDFRRHGQDMGACLQGFELQDLTNAALRESV-AGNHTGCLVIEVAAWTNAAAHLQKGDVVLSIDGHKLQNDKTVPFKDLDYINFEFLMSYHFVGDMMRVEVLRKEPVVEGEDGAALPPANTFHKLNLT-WEMQKRHPILPSRPQKQSFLMVGGLLFLPLSWEILDPDRSTRALRSFCESVRRISADE--EVVVLSEIFPHPINDGYFPPRLTRVLTFNDVPVRNLSHLTTMIEDCTEDWMRFKVDSNLQQMIVLDARENAVWNATQEICEIYSVP 623
            R    EG+F +  D  +      S P + +S G    G+ +       Q++ +       + ++ K+  T   P +  PW +Q   TS  T  AF + +  LLTN+HCV     + VKK G+   + A+VL +    D+AL+ V  + FWEG E +R   G + +L + VTVVGYP GG+ I +TKG+VSR+      H + E L IQIDAAINPGNSGGP  N+ GDC+G+A++      +ENIGY+IP  VV HFLED+ R+G+  G    G  LQ L N ALR  +   ++ G LV +V   ++ +  L++GDV++S DG ++ ++ TVPF+  + I F +L+S  F GD + + ++R    ++ +  A L P     +++L  + ++   P         S+L+V GL+F PLS  +++ +  +  L+   ++   ++  E  ++VVLS++  + +N GY      +VL  N   ++N+ HL  +++ C   ++ F+ + N   ++VL+ RE A  +A+  I + Y +P
Sbjct:   72 RRSKNEGAFANADDRSSTNEAGRSQPTSIKSFGLQRKGKGVLLDSKEQQVETSSFQDAAFLNAVVKVYCTHTAPDYSLPWQKQRQYTS--TGSAFMIGDGKLLTNAHCVEHDTQVKVKKRGDDTKYVAKVLARGVECDIALLSVESKDFWEGAEPLRF--GRLPRLQDAVTVVGYPLGGDTISVTKGVVSRIEVTSYAHGSSELLGIQIDAAINPGNSGGPAFNDDGDCIGVAFQVYRSDDAENIGYVIPTTVVSHFLEDYERNGKYCGFPCLGVLLQKLENPALRACLKVPSNEGVLVRKVEPTSDVSNVLKEGDVIVSFDGVRVGSEGTVPFRSSERIAFRYLISQKFSGDSVELGIIRGGEFMKVQ--AVLKP-----RVHLVPYHIEGGQP---------SYLIVAGLVFTPLSEPLIEEEEDSIGLKLLTKARYSLAKFEGQQIVVLSQVLANEVNIGYEDMSNDQVLKLNGTRIKNIHHLAHLVDSCKGKYLVFEFEDNF--LVVLE-RE-AALDASSSILKDYGIP 580