EWM23154.1, cds7515 (CDS) Nannochloropsis gaditana

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|585104035|gb|EWM23154.1| (carboxylesterase type b [Nannochloropsis gaditana])

HSP 1 Score: 1286.55 bits (3328), Expect = 0.000e+0
Identity = 623/623 (100.00%), Postives = 623/623 (100.00%), Query Frame = 0

Query:    1 MGLLKVFHFAKEDEPVIVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFLKVEGGDYNCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVDFNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNGACHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPNFQSGEEKKYLDIADAGSESTVLPDWPHLTASAPHQVMILDVPLSACRIQEVDLHLTFPELWKIVLKNARALWFHRPPPSGSKTERAMQSNL 623
            MGLLKVFHFAKEDEPVIVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFLKVEGGDYNCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVDFNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNGACHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPNFQSGEEKKYLDIADAGSESTVLPDWPHLTASAPHQVMILDVPLSACRIQEVDLHLTFPELWKIVLKNARALWFHRPPPSGSKTERAMQSNL
Sbjct:    1 MGLLKVFHFAKEDEPVIVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFLKVEGGDYNCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVDFNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNGACHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPNFQSGEEKKYLDIADAGSESTVLPDWPHLTASAPHQVMILDVPLSACRIQEVDLHLTFPELWKIVLKNARALWFHRPPPSGSKTERAMQSNL 623          

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|1020118695|ref|WP_063252524.1| (carboxylesterase/lipase family protein [Bacillus niacini])

HSP 1 Score: 235.343 bits (599), Expect = 1.278e-66
Identity = 179/553 (32.37%), Postives = 254/553 (45.93%), Query Frame = 0

Query:   16 VIVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFL---KVEGGDY----NCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVDFNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNGA---CHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPNFQSGEEKKYLDIADAGSESTVLPDWP 558
             IV++  G+++G +  D  V  ++GIP+A PP GS RFR PE   +WEG++    F   A Q    I+  F                  N I N+N             E+CL L V +P                  G + +  PVMVWIHGGAF  G+ S   Y    SF     VVVVTINYRLGILGFL   ++ G +Y    NCG+ DQVAALQWVQENI+AFGGDP NVT+FGESAG MS G L     A  LFH+ ILQSG+     S ++A  +A     AL +   +L  + ++P   L+ A +LV        M   PV+DG  L     +    G A+++  LI   + E  +F   F   + +  E ++ K+              FE     +V  I ++ GG  P S   Y        N +L  +I ++ A +       +   V++ RFD  +P   GA    HA+++ +VF ++   +        P    ++  +  +W NFAR  DPN ++                  LP+WP
Sbjct:    4 TIVESAHGKLQGEQIND--VFAWKGIPYAKPPVGSLRFRAPELPDSWEGIRDATSFSPVAPQPQREIMEFF-----------------GNDISNMN-------------EDCLYLNVWSP------------------GADDKKRPVMVWIHGGAFISGSGSSSWYDG-ASFAENGDVVVVTINYRLGILGFLHLGEIGGEEYATSGNCGIQDQVAALQWVQENISAFGGDPNNVTVFGESAGAMSIGVLLGFPSAQGLFHKAILQSGAAANVHSSDKATKIAGHLLGALQVEPTNLSKLEELPIEQLIQASDLVPP------MSLGPVMDGISLPRHPEEAIADGSAKDVTILIGTNKDEYNIF-SAFDPEWKNADETQVTKQ--------------FEKTFGPLVPVISSYLGGNKPLSQELY--------NKLLTMSIFTYPAQKLTELQVKQGAPVWMYRFDWETPVFGGALKSTHALEIPFVFNTLNTPNTENFTGSSPDRQLVAIQMHQSWINFARNSDPNTEN------------------LPEWP 458          

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|651986012|ref|WP_026696108.1| (carboxylesterase/lipase family protein [Bacillus kribbensis])

HSP 1 Score: 234.572 bits (597), Expect = 2.696e-66
Identity = 186/560 (33.21%), Postives = 250/560 (44.64%), Query Frame = 0

Query:   16 VIVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFLKVE---GGDY----NCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVD---FNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNG---ACHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPNFQSGEEKKYLDIADAGSESTVLPDWPHLTA 562
             IV+T  GRV+G    +  V V+RGIP+A PP G  RFR PEP  +WEG++    F   A Q    I+     +FL             N + N+N             E+CL L V +P                  G + +  PVMVWIHGGAF  G+ S   Y    SF +   VVVVTINYRLGILGFL +    G +Y    NCG+ DQVAALQWVQ+NIA FGGDP NVTIFGESAG MS G L     A  LFH+ ILQSG+     S E+A  +A     AL +   +L  +  +P   L+   NLV        M   PVVDG  L     +    G AR++  LI   + E  +F  +F   + +  E+              M    FE A            G   P     +   + +D   FN +L   I ++ A +       +   V++ RFD  +P + G   A HA+++ +V+ ++   +        P    L+  +  AW +FAR G PN                  +  LPDWP   A
Sbjct:    2 TIVRTSYGRVKGEHLDE--VLVWRGIPYAKPPVGPLRFRSPEPPDSWEGIRDASSFSPAAPQSKRDIM-----EFLG------------NDLSNMN-------------EDCLYLNVWSP------------------GADDKKRPVMVWIHGGAFVSGSGSSSWYDG-ASFSSLGDVVVVTINYRLGILGFLHLAEFGGKEYASSGNCGILDQVAALQWVQDNIAEFGGDPENVTIFGESAGAMSIGVLLGFPAAKGLFHKAILQSGAAANVHSSEKAAKIAKHLLSALQVEPANLSVLDKLPVEQLVEVSNLV------PAMSLGPVVDGVSLPKHPEEAIAEGSARDISILIGTNKDEWQIF-SVFDPQWKNADEK--------------MTAVFFEKAF-----------GPQAPLIAIQFMEDRPLDQDLFNKLLTMKIFTYPARQLAELQVKQGASVWMYRFDWETPVLGGALKATHALEIPFVWNTLDKANTENFTGSSPERQLLANQMHQAWISFAREGKPN------------------TGALPDWPRYDA 460          

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|1084589739|gb|OGO44643.1| (hypothetical protein A2Z05_05110 [Chloroflexi bacterium RBG_16_60_22])

HSP 1 Score: 226.483 bits (576), Expect = 4.277e-63
Identity = 193/541 (35.67%), Postives = 261/541 (48.24%), Query Frame = 0

Query:   13 DEPVIVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRS--CSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKG-VVVVTINYRLGILGFL---KVEGGDY----NCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQ-----NLVIRGKKCGIMPFQPVVDGDLLKD---RIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVDFNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMN---GACHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPN 532
            D P +V TK G++EG +  + G+ VFRGIP+AAPP G  R+R P+P   W GV+   +FG                                 PI   NP     +PRS    +E+CL L V TP                  G + R  PVMVWIHGGAF +G+ SQ ++    + LARKG VV+VTINYRLG+LGFL   ++ GG      N G+ DQVAAL+WV++NI AFGGDP N+T+FGESAG MS G L A   A  LFH+ IL+SG   T +  +EA  +     + L I       +  +  P LL         L   G+   +    PVVDG+++ D   R+I+    G A ++  ++     E  LF  + P     + E  + +R+   +  P  D+P   AA +      KA E  G P + AD  T    D    L F +P+    E   +  G+A   YL  F   SP M    GACHA+D+ +VFG         C +  P    LS  +  AW  FARTGDP+
Sbjct:    4 DTPALVATKSGKLEGSR--EGGLYVFRGIPYAAPPVGKLRWRPPQPLKPWSGVRPAREFG---------------------------------PIAPQNPLAGSIIPRSEEPQDEDCLFLNVWTP------------------GLDNRRRPVMVWIHGGAFTIGSGSQAMHN--GAGLARKGNVVIVTINYRLGLLGFLRLKELTGGGIPSTGNEGLLDQVAALEWVRDNIGAFGGDPANITVFGESAGAMSIGCLMAMPAARGLFHKAILESGVGSTAVPLDEATGVGERFVKELGIKSDDDRALRALTPPQLLDVDMRLRTGLAGPGEAVRLTAVAPVVDGEIIPDVPNRVINN---GSASDVKVIVGTNLEEFKLFAAMDPPGVKID-EAVMSRRLRAFV--PAGDIPRLVAAYR------KAREKRGEPATPADLLTAIQTD----LMFRMPALELVEAQRDN-GQAAYSYL--FTWESPVMGGMLGACHALDIGFVFGL---YDDAFCGSG-PEADKLSGCIQEAWLAFARTGDPS 466          

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|503862128|ref|WP_014096122.1| (carboxylesterase/lipase family protein [Bacillus coagulans] >gi|347583710|gb|AEO99976.1| Carboxylesterase type B [Bacillus coagulans 36D1] >gi|985291342|gb|KWZ76660.1| putative para-nitrobenzyl esterase [Bacillus coagulans] >gi|1004853295|gb|KYC63861.1| hypothetical protein B4100_0844 [Bacillus coagulans])

HSP 1 Score: 225.713 bits (574), Expect = 5.317e-63
Identity = 191/588 (32.48%), Postives = 267/588 (45.41%), Query Frame = 0

Query:   17 IVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFLKV--EGGDY----NCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVD-FNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNG---ACHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPNFQSGEEKKYLDIADAGSESTVLPDW-PHLTASAPHQVMILDVPLSACRIQEVDLHLTFPELW 593
            IV+T  G+  G + K  GV +++GIP+A PP G  RFR PE    W GVK C  FG  A Q   + ++ FL                 NP  N+             +E+CLNL + TP               G++G      PVMVWIHGGAF  GA S   Y    +F     VVVVTINYRLG LGFL +   GG+Y    NCG+ DQ+AAL+WV+ENIAAFGGDP  VTIFGESAG MS   L ++  A  LFH+ IL+SG+     + E A   A      L + K  +  + ++PA  L  A N          M   PV DG +L +   ++     A+++  LI   + E  LF    P          + KR          D  + +   QK  A  K W+      +    +T +  D   +   F  P+   A+T  +     +KV++ +FD  SP  NG   ACHA+++ +V+ +++         + P   +L+  +  AW  FA+ GDPN                   ++LP+W P+ T   P  +   D     CR+ E D H     LW
Sbjct:    5 IVETVFGKARGYEEK--GVQIWKGIPYAKPPIGPLRFRPPELPEPWAGVKDCTQFGPIAWQP-PVELMDFL----------------GNPAENM-------------DEDCLNLNIWTP---------------GADGERR---PVMVWIHGGAFANGAGSAPSYD-GSAFAKNGDVVVVTINYRLGALGFLYLGEMGGEYEASGNCGILDQIAALKWVKENIAAFGGDPDCVTIFGESAGAMSVAALLSSPAASGLFHKAILESGAANFTATPERAAKNARRILETLGLEKKDVAKLAEVPAKNLAEAVN------SLPFMSLLPVTDGIVLPEH-PERALENAAKDIPVLIGTNKDEYRLFTVFDP----------VWKR---------QDPKEMQDVFQKTFA--KYWDALSAKITDPSAFTQELYDRIMTYFVFTGPALKLADTRAQT---GEKVWMYQFDWESPVYNGTLKACHALEIPFVWHTLEQPGTENLTGNAPGRHALADQMHQAWIAFAQNGDPN------------------CSLLPEWPPYNTRQRPAMIFGQD-----CRV-EKDPHQAERALW 486          

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|33311865|gb|AAQ03995.1|AF417207_1 (esterase 54 [Bacillus niacini])

HSP 1 Score: 225.328 bits (573), Expect = 9.330e-63
Identity = 172/527 (32.64%), Postives = 244/527 (46.30%), Query Frame = 0

Query:   16 VIVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFL---KVEGGDY----NCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVDFNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNGA---CHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPN 532
             IV +  G+++G +    GVC ++G+P+A PP G+ RFR PE   +WEGV+    F   A Q    I+  F                  N I N+N             E+CL L V +P                  G + +  PVMVWIHGGAF  G+ S   Y    SF A+  VVVVTINYRLGILGFL   ++ G +Y    NCG+ DQVAALQWVQENIA+FGGDP NVT+FGESAG MS G L     A  LFH  ILQSG+     S E A  +A     AL +   +L  + ++    L+   +LV        M   PV+DG  L     +    G A+++  L+   + E  +F  +F   + +  E K+                 FE     +V  I  +  GG+   + D        FN +L   I ++ A +       +   V++ RFD  +P   GA    HA+++ +VF +++  +        P    ++  +   W NFA++G PN
Sbjct:    4 TIVGSVYGKLQGEQVD--GVCSWKGVPYAKPPVGALRFRAPERPDSWEGVRQATSFSPVAPQTQREIMEFF-----------------GNDISNMN-------------EDCLYLNVWSP------------------GADDKKRPVMVWIHGGAFVSGSGSSSWYDGA-SFAAQGDVVVVTINYRLGILGFLHLGEIGGEEYATSGNCGILDQVAALQWVQENIASFGGDPNNVTVFGESAGAMSIGVLLGFPSAQGLFHNAILQSGAAANVHSSETATKVAGHLLAALQVEPTNLSKLEELSVEQLIQVADLVPP------MSLGPVIDGVSLPKHPQEAIADGSAKDVSILVGTNKDEYNIF-SVFDPEWKNADEAKVTAL--------------FEKTFGPLVQVISKFIPGGL---NQDL-------FNKLLTDTIFTNPAQKLAELQVNQGTPVWMYRFDWETPVFGGALKSTHALEIPFVFNTLRTPNTENFTGSSPERQQIADQMHQRWINFAKSGHPN 448          

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|651962687|ref|WP_026684766.1| (carboxylesterase/lipase family protein [Bacillus coagulans])

HSP 1 Score: 223.787 bits (569), Expect = 2.711e-62
Identity = 196/588 (33.33%), Postives = 268/588 (45.58%), Query Frame = 0

Query:   17 IVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFLKV--EGGDY----NCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVD-FNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNG---ACHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPNFQSGEEKKYLDIADAGSESTVLPDW-PHLTASAPHQVMILDVPLSACRIQEVDLHLTFPELW 593
            IV+T  GRV G +  D GV +F+GIP+A PP G  RFR PEP   W G+K C  FG  A Q   + ++ FL                 NP  N++             E+CLNL + TP              +   GR     PVMVWIHGGAF  GA S   Y    +F     VVVVTINYRLG LGFL +   G +Y    NCG+ DQ+AAL+WV+ENIA+FGGDP  VTIFGESAG MS   L ++  A  LFH+ IL+SG+     + E A   A      L + K  +  + ++PA  L  A N          M   PV DG +L +   +K     A+++  LI   + E  LF    P          + +R          D  + +A  QK  A  K W+      +    +T +  D   +   F  P+   A+T  +     +KV++ RFD  SP  NG   ACHA+++ +V+ +++         +     +L+  +  AW  FA+ GDPN                   ++LP+W P+ T   P   MI D     CRI E D H     LW
Sbjct:    5 IVETVCGRVRGYE--DKGVQIFKGIPYAKPPIGPLRFRPPEPPEPWSGIKDCTQFGPIAWQP-PVELMDFL----------------GNPAENMS-------------EDCLNLNIWTP--------------EVENGRR----PVMVWIHGGAFANGAGSAPSYDG-SAFAQNGDVVVVTINYRLGALGFLYLGEMGEEYKASGNCGILDQIAALKWVKENIASFGGDPDCVTIFGESAGAMSVAALLSSPAARGLFHKAILESGAANFTATPERAANNARRILETLGLEKKDVAKLAEVPAKNLAEAVN------SLPFMSLLPVTDGIVLPEH-PEKVLENAAKDIPVLIGTNKDEYRLFTAFDP----------VWRR---------QDPREMQAVFQKTFA--KDWDALSTKMTDPSAFTQELYDRIMTYFVFTGPTLKLADTRAQT---GEKVWMYRFDWESPVYNGMLKACHALEIPFVWHTLEQPGTENLTGNALGRHALANRMHQAWIAFAKNGDPN------------------CSLLPEWPPYDTTQRP--TMIFD---QDCRI-EHDPHQAERTLW 486          

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|7546321|pdb|1C7J|A (Chain A, Pnb Esterase 56c8)

HSP 1 Score: 223.402 bits (568), Expect = 3.717e-62
Identity = 172/529 (32.51%), Postives = 251/529 (47.45%), Query Frame = 0

Query:   17 IVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKG-VVVVTINYRLGILGFLKVEGGDY----NCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMP--FQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVDFNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMH--SPTMNGACHAIDLAYVFGSVKPMSGGLCPADIPSNA-SLSAAVISAWTNFARTGDPNFQS 535
            IV T+ G+V+G    + GV  ++GIP+A PP G  RF+ PEP   WE V     +G    Q   ++ L++                               LPR    E+CL + V  P  P                  ++ LPVMVWIHGGAF LGA S+ +Y    S LA +G V+VVT+NYRLG  GF+ +   D     N G+ DQ AAL+WV+ENI+AFGGDP NVT+FGESAGGMS   L A   A  LF + I++SG+ +T +++E+A   AA   + L I++  L+ +  + A  LL A + +   +K  I    FQP +D   L +        G A  +  LI  TR E  LF   F ++    +++ L   + +SL  P+ +                  +   + P   +   +   D    L F  P    A     A      V++ RFD H   P  N A HA++L +VFG++  +   +  A+I      LS  + SAW  FA+TG+P+ ++
Sbjct:    5 IVTTQYGKVKGTT--ENGVHKWKGIPYAKPPVGQWRFKAPEPPEVWEDVLDATAYGPVCPQPSDLLSLSY-----------------------------TELPRQS--EDCLYVNVFAPDTP------------------SQNLPVMVWIHGGAFYLGAGSEPLYD--GSKLAAQGEVIVVTLNYRLGPFGFMHLSSFDEAYSDNLGLLDQAAALKWVRENISAFGGDPDNVTVFGESAGGMSIAALLAMPAAKGLFQKAIMESGASRT-MTKEQAASTAAAFLQVLGINESQLDRLHTVAAEDLLKAADQLRIAEKENIFQLFFQPALDPKTLPEEPEKSIAEGAASGIPLLIGTTRDEGYLF---FTSDSDVRSQETLDAALEYSLGKPLAE------------------KAADLYPRSLESQIHMVTD----LLFWRP----AVAFASAQSHYAPVWMYRFDWHPEKPPYNKAFHALELPFVFGNLDGLER-MAKAEITDEVKQLSHTIQSAWITFAKTGNPSTEA 449          

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|518550593|ref|WP_019720800.1| (carboxylesterase/lipase family protein [Bacillus coagulans])

HSP 1 Score: 223.016 bits (567), Expect = 4.999e-62
Identity = 192/588 (32.65%), Postives = 266/588 (45.24%), Query Frame = 0

Query:   17 IVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFLKV--EGGDY----NCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVD-FNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNG---ACHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPNFQSGEEKKYLDIADAGSESTVLPDW-PHLTASAPHQVMILDVPLSACRIQEVDLHLTFPELW 593
            IV+   G+V G + K  GV +F+GIP+A PP G  RFR PEP   W G+K C  FG  A Q   + ++ FL                 NP  N+             +E+CLNL + TP                  G +    PVMVWIHGGAF  GA S   Y    +F     VVVVTINYRLG LGFL +   GG+Y    NCG+ DQ+AAL+WV++NIAAFGGDP  VTIFGESAG MS   L ++  A  LFH+ IL+SG+     + E A          L + K  +  + ++PA  L  A N          M   PV DG +L +   +K    VA+++  LI   + E  LF    P  +  +  Q++Q                  A  QK  A  K W+      +    +T +  D   +   F  P+   A+T  +     +K+++ RFD  SP  NG   ACHA+++ +V+ +++         + P   +L+  +  AW  F +  DPN                   ++LP+W P+ T   P   MI D     CRI E D H     LW
Sbjct:    5 IVEAVCGKVRGYEEK--GVQIFKGIPYAKPPIGPLRFRPPEPPEPWSGIKDCTQFGPIAWQP-PVELMDFL----------------GNPAENM-------------DEDCLNLNIWTP------------------GTDRGRRPVMVWIHGGAFANGAGSAPSYD-GSAFAKNGDVVVVTINYRLGALGFLYLGEMGGEYKASGNCGILDQIAALKWVKDNIAAFGGDPDCVTIFGESAGAMSVAALLSSPAARGLFHKAILESGAANFTATPERAAKNTRRILETLGLEKKDVAKLAEVPAKNLAEAVN------SLPFMSLLPVTDGIVLPEH-PEKVLENVAKDIPVLIGTNKDEYRLFTAFDPV-WRKQDPQEMQ------------------AVFQKTFA--KDWDALSAKITDPSAFTQELYDRIMTYFVFTGPALKLADTRAQT---GEKMWMYRFDWESPVYNGMLKACHALEIPFVWHTLEQPGTENLTGNAPGRHALADRMHQAWIAFTKNDDPN------------------CSLLPEWPPYNTRQRP--TMIFD---QDCRI-EHDPHQAERALW 486          

BLAST of EWM23154.1 vs. NCBI_GenBank
Match: gi|737196983|ref|WP_035182471.1| (carboxylesterase/lipase family protein [Bacillus coagulans] >gi|685885165|gb|KGB30504.1| carboxylesterase [Bacillus coagulans] >gi|757440977|gb|AJO24304.1| carboxylesterase type B [Bacillus coagulans] >gi|850400921|gb|AKN54225.1| putative esterase [Bacillus coagulans] >gi|1002352549|gb|KXT19080.1| carboxylesterase [Bacillus coagulans])

HSP 1 Score: 220.705 bits (561), Expect = 3.940e-61
Identity = 189/589 (32.09%), Postives = 265/589 (44.99%), Query Frame = 0

Query:   17 IVKTKQGRVEGRKRKDTGVCVFRGIPFAAPPTGSRRFRKPEPYPAWEGVKLCEDFGKTAVQGLSIIVLAFLPKFLAALAWGCLWLTNSNPIYNLNPKRPMALPRSCSEEECLNLTVETPFLPLEGELREDHEGDGSEGRETRLLPVMVWIHGGAFQLGANSQFVYQPPESFLARKGVVVVTINYRLGILGFL---KVEGGDY----NCGMWDQVAALQWVQENIAAFGGDPGNVTIFGESAGGMSCGFLSATDFAGRLFHRVILQSGSIQTGLSREEAGWLAAEGARALDISKCSLETMGDIPAPTLLAAQNLVIRGKKCGIMPFQPVVDGDLLKDRIIDKWRAGVARNLDFLISYTRHEELLFLKLFPANYTSETEQKLQKRVTFSLSPPVMDVPDFEAAAQKVVAEIKAWEGGGIPPSHADYWTYKAVD-FNSVLKFAIPSHIAAETLTEAPGRAKKVYLCRFDMHSPTMNG---ACHAIDLAYVFGSVKPMSGGLCPADIPSNASLSAAVISAWTNFARTGDPNFQSGEEKKYLDIADAGSESTVLPDW-PHLTASAPHQVMILDVPLSACRIQEVDLHLTFPELW 593
            IV+T  G+  G + K  GV +++GIP+A PP G  RFR PE    W GVK C  FG  A Q   + ++ FL                 NP  N+             +E+CLNL + TP               G++G      PVMVWIHGGAF  GA S   Y    +F     VVVVTINYRLG LGFL   ++ G  Y    NCG+ DQ+AAL+WV+ENIAAFGGDP  VTIFGESAG MS   L ++  A  LFH+ IL+SG+     + E A          L + K  +  + ++PA  L  A N          M   PV DG +L +   ++     A+++  LI   + E  LF    P          + KR          D  + +   QK  A  K W+      +    +T +  D   +   F  P+   A+T  +     +KV++ +FD  SP  NG   ACHA+++ +V+ +++         + P   +L+  +  AW  FA+ GDPN                   ++LP+W P+ T   P  +   D     CR+ E D H     LW
Sbjct:    5 IVETVFGKARGYEEK--GVQIWKGIPYAKPPIGPLRFRPPELPEPWAGVKDCTQFGPIAWQP-PVELMDFL----------------GNPAENM-------------DEDCLNLNIWTP---------------GADGERR---PVMVWIHGGAFANGAGSAPSYD-GSAFAKNGDVVVVTINYRLGALGFLYLGEMGGEKYEASGNCGILDQIAALKWVKENIAAFGGDPDCVTIFGESAGAMSVAALLSSPAASGLFHKAILESGAANFTATPERAAKNTRRILETLGLEKKDVAKLAEVPAKNLAEAVN------SLPFMSLLPVTDGIVLPEH-PERALENAAKDIPVLIGTNKDEYRLFTVFDP----------VWKR---------QDPKEMQDVFQKTFA--KYWDALSAKITDPSAFTQELYDRIMTYFVFTGPALKLADTRAQT---GEKVWMYQFDWESPVYNGTLKACHALEIPFVWHTLEQPGTENLTGNAPGRHALADRMHQAWIAFAQNGDPN------------------CSLLPEWPPYNTRQRPAMIFGQD-----CRV-EKDPHQAERALW 487