EWM23344.1, cds7324 (CDS) Nannochloropsis gaditana

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|585104352|gb|EWM23344.1| (phosphatidylinositide phosphatase sac1 [Nannochloropsis gaditana])

HSP 1 Score: 1952.56 bits (5057), Expect = 0.000e+0
Identity = 966/966 (100.00%), Postives = 966/966 (100.00%), Query Frame = 0

Query:    1 MPGGGVVGVRTKKRTKMPLNGGMMWGKTILTPPPPSSSPASSLLSSPKGPITTRKHSSLLYLTVKPDYLVIRAPCGPHSPKTQQGLDPVLVLRAPPSTPRTGPRWPQADGDPVSPPPDTRGAGSNSVTVEVKDFQTAVRDGDIVGSEEEREIQGILGLVYLLGGAYLLVIEASEIVGTSPRGSNLTDKTSSFSIHRVQKMGMLPVSRRQQTGPHPQQHPALSSPGQRCDEERYLRMLQAPVVAGSLYFSPRYPLHRSLQENIRRCPGLDFESTAPAFWGEEAATGREGGGLGDVDSPAYALMAKNGSSRRGTDSRAASSSSTSSFSSSSSSSSNLTSPSPSTSLPFLWNGFLLSNLGPARLFWGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALEREGEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEYCPRGPSPTHAPISPFPPYRLPFTPGGIFFTLSVALQELPPFQLTLQAGALLTEAVVSLAQALVGSAATASSSLGGRDESPSAPPSWWVLAVFSYMMFLLFWVALWECVLFLGPFATLRTLRHHRYLLTDRPRLVEKDGEAGKDGNGREAT 966
            MPGGGVVGVRTKKRTKMPLNGGMMWGKTILTPPPPSSSPASSLLSSPKGPITTRKHSSLLYLTVKPDYLVIRAPCGPHSPKTQQGLDPVLVLRAPPSTPRTGPRWPQADGDPVSPPPDTRGAGSNSVTVEVKDFQTAVRDGDIVGSEEEREIQGILGLVYLLGGAYLLVIEASEIVGTSPRGSNLTDKTSSFSIHRVQKMGMLPVSRRQQTGPHPQQHPALSSPGQRCDEERYLRMLQAPVVAGSLYFSPRYPLHRSLQENIRRCPGLDFESTAPAFWGEEAATGREGGGLGDVDSPAYALMAKNGSSRRGTDSRAASSSSTSSFSSSSSSSSNLTSPSPSTSLPFLWNGFLLSNLGPARLFWGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALEREGEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEYCPRGPSPTHAPISPFPPYRLPFTPGGIFFTLSVALQELPPFQLTLQAGALLTEAVVSLAQALVGSAATASSSLGGRDESPSAPPSWWVLAVFSYMMFLLFWVALWECVLFLGPFATLRTLRHHRYLLTDRPRLVEKDGEAGKDGNGREAT
Sbjct:    1 MPGGGVVGVRTKKRTKMPLNGGMMWGKTILTPPPPSSSPASSLLSSPKGPITTRKHSSLLYLTVKPDYLVIRAPCGPHSPKTQQGLDPVLVLRAPPSTPRTGPRWPQADGDPVSPPPDTRGAGSNSVTVEVKDFQTAVRDGDIVGSEEEREIQGILGLVYLLGGAYLLVIEASEIVGTSPRGSNLTDKTSSFSIHRVQKMGMLPVSRRQQTGPHPQQHPALSSPGQRCDEERYLRMLQAPVVAGSLYFSPRYPLHRSLQENIRRCPGLDFESTAPAFWGEEAATGREGGGLGDVDSPAYALMAKNGSSRRGTDSRAASSSSTSSFSSSSSSSSNLTSPSPSTSLPFLWNGFLLSNLGPARLFWGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALEREGEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEYCPRGPSPTHAPISPFPPYRLPFTPGGIFFTLSVALQELPPFQLTLQAGALLTEAVVSLAQALVGSAATASSSLGGRDESPSAPPSWWVLAVFSYMMFLLFWVALWECVLFLGPFATLRTLRHHRYLLTDRPRLVEKDGEAGKDGNGREAT 966          

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|553194539|ref|XP_005855983.1| (phosphatidylinositide phosphatase sac1 [Nannochloropsis gaditana CCMP526] >gi|422293091|gb|EKU20392.1| phosphatidylinositide phosphatase sac1 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1899.79 bits (4920), Expect = 0.000e+0
Identity = 948/972 (97.53%), Postives = 953/972 (98.05%), Query Frame = 0

Query:    1 MPGGGVVGVRTKKRTKMPLNGGMMWGKTILTPPPPSSSPASSLLSSPKGPITTRKHSSLLYLTVKPDYLVIRAPCGPHSPKTQQGLDPVLVLRAPPSTPRTGPRWPQADGDPVSPPPDTRGAGSNSVTVEVKDFQTAVRDGDIVGSEEEREIQGILGLVYLLGGAYLLVIEASEIVGTSPRGSNLTDKTSSFSIHRVQKMGMLPVSRRQQTGPHPQQHPALSSPGQRCDEERYLRMLQAPVVAGSLYFSPRYPLHRSLQENIRRCPGLDFESTAPAFWGEEAATGREGGGLGDVDSPAYALMAKNGSSRRGTDSRAASSSSTSSFSSSSSSSSNLTSPSPSTSLPFLWNGFLLSNLGPARLFWGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALEREGEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEYCPRGPSPTHAPISPFPPYRLPFTPGGIFFTLSVALQELPPFQLTLQAGALLTEAVVSLAQALVGSAATASSSLGGRDESPSAPPSWWVLAVFSYMMFLLFWVALWECVLFLGPFATLRTLRHHRYLLTDRPRLVE------KDGEAGKDGNGREAT 966
            MPGGGVVGVRTKKRTKMPLNGGMMWGKTILTPPPPSSSPASSLLSSPKGPITTRKHSSLLYLTVKPDYLVIRAPCGPHSPKTQQGLDPVLVLRAPPSTPRTGPRWPQADGDPVSPPPDTRGAGS+SVTVEVKDFQTAVRDGDIVGSEEEREIQGILGLVYLLGGAYLLVIEASEIVGTSPRGSNLTDKTSSFSIHRVQKMGMLPVSRRQQTGPHPQQHPALSSPGQRCDEERYLRMLQAPVVAGSLYFSPRYPLHRSLQENIRRCPGLDFESTAP F G     G  GGG+GDVDSPAYALMAKNGSSRRGTDSRAASSSSTSSFSSSSSSSSNLTSPSPSTSLPFLWNGFLLSNLGPARLFWGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALER GEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGG+PRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRI+D GKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEYCPRGPSPTHAPISPFPPYRLPFTPGG+F TLSVALQELPPFQLTLQAGALLTEAVVSLAQALVGSAATASSSLGG DESPSAPPSWWVLAVFSYMMFLLFWVALWECVLFLGPFATLRTLRHHRYLLTDRPRLVE      KDGEAGKDGNGREAT
Sbjct:   80 MPGGGVVGVRTKKRTKMPLNGGMMWGKTILTPPPPSSSPASSLLSSPKGPITTRKHSSLLYLTVKPDYLVIRAPCGPHSPKTQQGLDPVLVLRAPPSTPRTGPRWPQADGDPVSPPPDTRGAGSDSVTVEVKDFQTAVRDGDIVGSEEEREIQGILGLVYLLGGAYLLVIEASEIVGTSPRGSNLTDKTSSFSIHRVQKMGMLPVSRRQQTGPHPQQHPALSSPGQRCDEERYLRMLQAPVVAGSLYFSPRYPLHRSLQENIRRCPGLDFESTAPPFGGRRRPRGGRGGGVGDVDSPAYALMAKNGSSRRGTDSRAASSSSTSSFSSSSSSSSNLTSPSPSTSLPFLWNGFLLSNLGPARLFWGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALERGGEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGGTPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIHDAGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEYCPRGPSPTHAPISPFPPYRLPFTPGGVFSTLSVALQELPPFQLTLQAGALLTEAVVSLAQALVGSAATASSSLGGWDESPSAPPSWWVLAVFSYMMFLLFWVALWECVLFLGPFATLRTLRHHRYLLTDRPRLVEKDGEAGKDGEAGKDGNGREAT 1051          

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|1173942645|gb|OQR91610.1| (phosphatidylinositide phosphatase SAC1 [Achlya hypogyna])

HSP 1 Score: 218.779 bits (556), Expect = 1.417e-56
Identity = 168/509 (33.01%), Postives = 229/509 (44.99%), Query Frame = 0

Query:  346 FLWNGFLLSNLGPARLF---WGTACISGYA-------VSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVP-------LDEFGEEGRGA-LEREGEEGA-----------KTEATKSRSTPAPDRE-----DDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHAL----SSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEY 816
            F WN  +L     ++L    W    I+GY        +S  +P                 +RRS R  GTRF  RG +K+G  ANF ETEV++  P+G+          + S+VQ+RGSIPL W Q  TL++ P  R +       SS+ S+                    AH +Q+ + Y  I +VNL+D  G S   + QA LG   Q+  +K  A          L   G  G  A +ER    G+                  RSTP+ D          + S FAE         ++  WFDFH EC    +WG L + L+ Q++   S         YD                           W EA  +  VL++Q+GV R NCMDNLDRTNVV S++  R +  AL    +++     +P PA  E      W +NADA+S +YAGT ALKTDF  TG+RTI G L DG+ +VTR Y+NNF DG RQD + L++G Y
Sbjct:  191 FFWNKPVLEPFFRSKLKLEKWIVPVINGYVKVMPNVKISADVP----------PFDFYFFTRRSWRRVGTRFNVRGVDKDGHTANFAETEVLMRRPDGS----------VCSYVQIRGSIPLYWDQMVTLKYMPRTRYAF------SSTESIVDWNELAFR-----------AHFDQIIAKYGHITVVNLIDKAGKSATVRDQAQLGSAYQKYTKKYNAAQADGSNPPALQHQGSSGDDAPMERSDRAGSFLGDSGGLVPPSPRLRFRRSTPSVDNTPVPAPTVVKPSLFAEP--------ITYVWFDFHHECRK-MQWGNL-SKLMDQVNDCFSK--------YD---------------------------WFEADRDGRVLKKQKGVFRVNCMDNLDRTNVVMSLIGRRAMLTALGVNTTNISNWLDSPFPA-FEVAFKNAWADNADAVSVMYAGTGALKTDFTRTGKRTIAGALQDGINSVTRYYLNNFSDGLRQDAMDLFVGNY 616          

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|669136862|ref|XP_008603999.1| (hypothetical protein SDRG_00306 [Saprolegnia diclina VS20] >gi|530744334|gb|EQC42576.1| hypothetical protein SDRG_00306 [Saprolegnia diclina VS20])

HSP 1 Score: 216.083 bits (549), Expect = 1.567e-55
Identity = 167/511 (32.68%), Postives = 229/511 (44.81%), Query Frame = 0

Query:  346 FLWNGFLLSNLGPARLF---WGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYV-------PLDEFGE--EGRGALEREGEEGAKT----------------EATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGA---LAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEYCP--RGPSP 823
            F WN  +L     ++L    W    I+GY   + +P +        A      +RRS R  GTRF  RG +K+G  ANF ETEV++  P+G           + S+VQ+RGSIPL W Q  TL++ P  R +       SSS S+                    AH +Q+ + Y  I +VNL+D  G S   + QA LG   Q+  +K  A         PL       +    LER    G+                   +T S  T +P      + S FAE         ++  WFDFH EC    +WG L + L+ Q++   S         YD                           W EA  +  VL++Q+GV R NCMDNLDRTNVV S++  R +  AL+   A     L     + E +    W +NADA+S +YAGT ALKTDF  TG+RT+ G + DG+ +VTR Y+NNF DG RQD + L++G Y    R  SP
Sbjct:  191 FFWNKPVLEPFFRSKLKLEKWIVPVINGYV--KVMP-NVKLSADVPAFDFFFFTRRSWRRIGTRFNVRGVDKDGHTANFAETEVLMRKPDGG----------ISSYVQIRGSIPLYWDQMVTLKYMPRTRYAF------SSSESIVDWNELAFR-----------AHFDQIIAKYGHITVVNLIDKAGKSATVRDQAQLGTAYQKYAKKYNAQSVDGSAPSPLQTASSSLDDDKPLERSDRSGSFLGDSGGLIPPSPRLRFRRSTPSVETVSPPAALSPKPSLFAEP--------ITYVWFDFHHECRK-MQWGNL-SKLMDQVNDCFSK--------YD---------------------------WFEADRDGRVLKKQKGVFRVNCMDNLDRTNVVMSLIGRRAMLQALNVNTANITNWLDSPFEAFELQFKNAWADNADAVSVMYAGTGALKTDFTRTGKRTLAGAIQDGVNSVTRYYLNNFSDGIRQDAMDLFVGNYAVDRRADSP 626          

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|813185421|ref|XP_012206882.1| (hypothetical protein SPRG_11310 [Saprolegnia parasitica CBS 223.65] >gi|641528114|gb|KDO22358.1| hypothetical protein SPRG_11310 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 213.001 bits (541), Expect = 1.297e-54
Identity = 167/509 (32.81%), Postives = 225/509 (44.20%), Query Frame = 0

Query:  346 FLWNGFLLSNLGPARLF---WGTACISGYA-------VSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYV-------PLDEFGE--EGRGALERE-------GEEGAKT---------EATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGA---LAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEY 816
            F WN  +L     ++L    W    I+GY        VS  +P                 +RRS R  GTRF  RG +K+G  ANF ETEV++  P+G           + S+VQ+RGSIPL W Q  TL++ P  R +       SSS S+                    AH +Q+ + Y  I +VNL+D  G S   + QA LG   Q+  +K  A         PL       +    LER        GE G             +T S  T +P      + S FAE         ++  WFDFH EC   + WG L + L+ Q++   S         YD                           W EA  +  VL++Q+GV R NCMDNLDRTNVV S++  R +  AL    A     L     + E +    W +NADA+S +YAGT ALKTDF  TG+RT+ G + DG+ +VTR Y+NNF DG RQD + L++G Y
Sbjct:  191 FFWNKPVLEPFFRSKLKLEKWIVPVINGYVKVMPNVKVSADVP----------PFDFFFFTRRSWRRIGTRFNVRGVDKDGHTANFAETEVLMRKPDGG----------ICSYVQIRGSIPLYWDQMVTLKYMPRTRYAF------SSSESIVDWNELA-----------FRAHFDQIIAKYGHITVVNLIDKAGKSATVRDQAQLGTAYQKYAKKYNAQSADGSAPSPLQTASSSLDDDKPLERSDRSGSFLGESGGLIPPSPRLRFRRSTPSVETVSPPAALSPKPSLFAEP--------ITYVWFDFHHECRKMQ-WGNL-SKLMDQVNDCFSK--------YD---------------------------WFEADRDGRVLKKQKGVFRVNCMDNLDRTNVVMSLIGRRAMLQALGVNTANITNWLDSPFEAFELQFKNAWADNADAVSVMYAGTGALKTDFTRTGKRTLAGAIQDGVNSVTRYYLNNFSDGIRQDAMDLFVGNY 617          

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|1173948823|gb|OQR96909.1| (phosphatidylinositide phosphatase SAC1 [Thraustotheca clavata])

HSP 1 Score: 206.068 bits (523), Expect = 3.010e-52
Identity = 151/478 (31.59%), Postives = 219/478 (45.82%), Query Frame = 0

Query:  363 WGTACISGYAVSRSIPLDGARKG-AGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGA------LEREGEEGAKTEATKSRSTPAPD-------REDDGRTSAFAERKGKGGSPR-------VSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGA---LAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEY 816
            W    I+GY   + +P    R G    +      +RRS    GTRF  RG +K+G  ANF ETE+++  P+G+          + S+VQ+RGSIPL W Q  TL++ P  R +       SS+ S+                    AH +Q+ + Y  I ++NL+D  G S   + QA LG   Q+  +K  A        ++  G+      ++ + E   ++     +++P P        R+    T   +        P        ++  WFDFH EC +  KW  L + L+ Q+                       G C  K              W EA  +  VL++Q+GV R NCMDNLDRTNVV S++A R +  AL    AG    L     S E +    W +NADA+S +YAGT ALKTDF  TG+R + GM+ DG+ +VTR Y+NNF DG RQD + L++  Y
Sbjct:  210 WIVPVINGYV--KVMP--NIRLGPDAPSFDFFFFTRRSWHRVGTRFNVRGVDKDGHTANFAETEMLMRRPDGS----------VCSYVQIRGSIPLYWDQMVTLKYMPRTRYAY------SSTESIVDWNELA-----------FRAHFDQIIAKYGHITVINLIDKTGKSATVRDQAQLGSAFQKYSKKYNA--------QQADGSSPSSSGVDEQIERSDRSSFIGEQNSPIPASPLRNILRKQTPSTDLTSSSSSLSPKPLSSLFPEPITYVWFDFHHECRN-MKWSNL-SKLMDQV-----------------------GDCFSKYN------------WFEADRDGRVLKKQKGVFRVNCMDNLDRTNVVMSLLARRAMLQALGVNTAGITNWLDSPFDSFEVQFKNAWADNADAVSIMYAGTGALKTDFTRTGKRGLPGMIQDGINSVTRYYLNNFTDGIRQDAMDLFVANY 611          

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|470518078|ref|XP_004353285.1| (hypothetical protein ACA1_196090 [Acanthamoeba castellanii str. Neff] >gi|440802831|gb|ELR23757.1| hypothetical protein ACA1_196090 [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 201.06 bits (510), Expect = 2.531e-51
Identity = 177/611 (28.97%), Postives = 257/611 (42.06%), Query Frame = 0

Query:  346 FLWNGFLLSNLGPARLF-WGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALEREGEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLR----HA-LSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEYCPRGPSPTHAPISPFPPYRLPFTPGGIFFTLSVALQELPPFQLTLQAGALLTEAVVSLAQALVGSAATASSSLGGRDESPSAPPSWWVLAVFSYMMFLLFWVALWECVLFLGPFATLRTLRHHRYLLTDRPRLV 950
            F WN +L  +   ++L  W T  + GY     + ++      G      L+SRR T+  GTR++ RGA+++G VANFVETE +L +P   R         + S VQ RGSIP+ WSQ P + ++P  RL+             A+      + +         AH++ LT +Y   +L+NL++  G      A+A +G+    E EK      +  +G+E                                                V   WFDFH EC   +      ++L+ Q+   +   G+ +                    T   E                 +R+QEGV RTNC+DNLDRTNVVQS +A  VL     HA +   P+ ++A  PA   +    +W  NADA+S  YAGT ALKTD+  TG+R+ KG+L DGL + TR Y+NNF DG+RQD   L+LG Y   G SP     SPF                        P   TL   ALL   V+ LA  +                +P   P      ++ +M+ ++FW      V  +G +   R L+ +   L DRP+LV
Sbjct:  153 FFWNRYLAQDFISSQLDGWVTPVMLGY-----VQIESHCTVNGHRFDYALISRRHTKRAGTRYHIRGADEQGHVANFVETEQVLVVPAQDR---------IYSFVQTRGSIPVFWSQAPDITYKPKPRLT-------------ATEKRNAAAFK---------AHMDDLTRHYSRHVLINLINHKG------AEAVIGK----EYEK-----QVQLYGDES-----------------------------------------------VRYVWFDFHHECRKMRFEN--VSNLIQQVKPEIEQFGYFL--------------------TENNE----------------AVRKQEGVFRTNCIDNLDRTNVVQSTLAREVLNAQLLHAGVFQRPSESIADHPA-FRQIFNHVWANNADAISEQYAGTGALKTDYTRTGKRSFKGVLNDGLNSATRYYLNNFKDGFRQDAYDLFLGNYVV-GQSPR----SPFA-------------------HNYRPVATTLFVIALLIGVVMLLASVV----------------APILQPEGTQSIIYQFMV-IVFW-----AVAMVGAY---RVLKLYGRDLVDRPQLV 577          

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|936592921|ref|XP_014209737.1| (PREDICTED: phosphatidylinositide phosphatase SAC1 [Copidosoma floridanum] >gi|936592923|ref|XP_014209738.1| PREDICTED: phosphatidylinositide phosphatase SAC1 [Copidosoma floridanum] >gi|936592925|ref|XP_014209739.1| PREDICTED: phosphatidylinositide phosphatase SAC1 [Copidosoma floridanum] >gi|936592927|ref|XP_014209740.1| PREDICTED: phosphatidylinositide phosphatase SAC1 [Copidosoma floridanum])

HSP 1 Score: 191.045 bits (484), Expect = 5.900e-48
Identity = 145/473 (30.66%), Postives = 207/473 (43.76%), Query Frame = 0

Query:  346 FLWNGFLLSNL--GPARLFWGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALEREGEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEY 816
            F+WN +LL +L   P    +    I G+    ++ ++G     G      ++SRRS    GTR + RG +  G V+NFVETE I+     A G++        S VQ RGSIPL W Q P L+++P     P L +  S  ++                    A H E    +Y   +L+NL+D  G      A+A L +  ++ V++L                      +  K EA                                    FDFH ECG   +W RL+  L+A++       G+ ++                   TR+                 ++L  Q+G+ RTNC+D LDRTNVVQSM+A RVL   LS L       +  SLE     +W +NAD +S  Y+GT ALKTDF  TG+RT  G + DG+ ++TR Y NNF DG+RQD +SL+LG Y
Sbjct:  168 FIWNSYLLQDLVARPEHHKFCLPIIHGFLSLNTVVVNGISFNWG------IISRRSVHRAGTRLFSRGIDANGNVSNFVETEQIVE----ANGSKS-------SFVQTRGSIPLFWQQTPNLKYKP----KPVLASNESHLIA-------------------CARHFESQIFHYGKQILINLIDHKG------AEATLEKAYRDIVQELK--------------------NQNIKYEA------------------------------------FDFHAECGK-MQWHRLEI-LMARLAHDQEQMGYFLL-------------------TRDN----------------VLLSVQDGIFRTNCIDCLDRTNVVQSMLAKRVLTDTLSKLEISRNMAEHPSLEYLFKQVWADNADIISIQYSGTSALKTDFTRTGKRTKMGAIKDGVNSLTRYYKNNFTDGFRQDAISLFLGRY 501          

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|380021582|ref|XP_003694641.1| (PREDICTED: phosphatidylinositide phosphatase SAC1 [Apis florea] >gi|820851479|ref|XP_012343282.1| PREDICTED: phosphatidylinositide phosphatase SAC1 [Apis florea])

HSP 1 Score: 186.422 bits (472), Expect = 2.388e-46
Identity = 148/473 (31.29%), Postives = 201/473 (42.49%), Query Frame = 0

Query:  346 FLWNGFLLSNLG--PARLFWGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALEREGEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEY 816
            F+WN +LL +L   P +  +    I G+     I + G+      +  L ++SRRS    GTR + RG +  G V+N+VETE ++       G+R       +S VQ RGSIPL W Q P L+++P     P L T                        +  A H E    +Y   +L+NL+D  GS              +  +EK  AY             ++R   E  + E+                                    FDFH EC   + W RL   L    D L                            T E E+ G   + E+ +    +L  Q+GV RTNC+D LDRTNVVQSM+A RVL   LS L       D  S+E     +W +NAD +S  Y+GT ALKTDF  TG+RT  G + DGL ++TR Y NNF DGYRQD + L+LG Y
Sbjct:  168 FVWNAYLLQDLTSRPEQYKFCLPIIHGFVSLNIITVTGS-----TSFNLGIVSRRSVHRAGTRLFSRGIDSTGNVSNYVETEQLVEF----NGHR-------MSFVQTRGSIPLFWYQAPNLKYKP----KPQLGTHEDHQ-------------------TACARHFETQIFHYGKQILINLIDHMGS--------------EGTLEK--AY----------HNVVQRINNENVRYES------------------------------------FDFHAECRRLR-WDRLNMLL----DRL----------------------------THELEQMGYFLLLEDGT----LLSAQDGVFRTNCIDCLDRTNVVQSMIAKRVLNDVLSRLQILRKIEDHPSVEEHFKRIWADNADIISIQYSGTGALKTDFTRTGKRTKLGAMRDGLNSLTRYYKNNFTDGYRQDSLDLFLGRY 502          

BLAST of EWM23344.1 vs. NCBI_GenBank
Match: gi|1035597609|ref|XP_016906172.1| (PREDICTED: phosphatidylinositide phosphatase SAC1 [Apis cerana] >gi|1035597611|ref|XP_016906173.1| PREDICTED: phosphatidylinositide phosphatase SAC1 [Apis cerana])

HSP 1 Score: 186.037 bits (471), Expect = 3.023e-46
Identity = 148/473 (31.29%), Postives = 201/473 (42.49%), Query Frame = 0

Query:  346 FLWNGFLLSNLG--PARLFWGTACISGYAVSRSIPLDGARKGAGEAARLTLLSRRSTRMQGTRFYRRGANKEGAVANFVETEVILALPEGARGNREGPETLLLSHVQVRGSIPLTWSQEPTLEWQPCVRLSPGLPTPPSSSLSLASHGSGGGSGRGGLQVSVTAAHLEQLTSNYRDILLVNLVDSGGSSKHSQAQAALGRRLQEEVEKLAAYVPLDEFGEEGRGALEREGEEGAKTEATKSRSTPAPDREDDGRTSAFAERKGKGGSPRVSLTWFDFHKECGSYKKWGRLKTHLLAQIDALLSSQGWTMVRIYDVGKQVGQGGCADKEKTREGERAGGQGVWEEASAEEIVLREQEGVVRTNCMDNLDRTNVVQSMVAGRVLRHALSSLPAGALAPDPASLERELTTLWVENADALSTLYAGTPALKTDFVLTGRRTIKGMLLDGLYAVTRLYINNFWDGYRQDCVSLWLGEY 816
            F+WN +LL +L   P +  +    I G+     I + G+      +  L ++SRRS    GTR + RG +  G V+N+VETE ++       G+R       +S VQ RGSIPL W Q P L+++P     P L T                        +  A H E    +Y   +L+NL+D  GS              +  +EK  AY             ++R   E  + E+                                    FDFH EC   + W RL   L    D L                            T E E+ G   + E+ +    +L  Q+GV RTNC+D LDRTNVVQSM+A RVL   LS L       D  S+E     +W +NAD +S  Y+GT ALKTDF  TG+RT  G + DGL ++TR Y NNF DGYRQD + L+LG Y
Sbjct:  168 FVWNAYLLQDLTSRPEQYKFCLPIIHGFVSLNIITVTGS-----TSFNLGIVSRRSVHRAGTRLFSRGIDSTGNVSNYVETEQLVEF----NGHR-------MSFVQTRGSIPLFWYQAPNLKYKP----KPQLGTHEDHQ-------------------TACARHFETQIFHYGKQILINLIDHMGS--------------EGMLEK--AY----------HNVVQRINNENVRYES------------------------------------FDFHAECRRLR-WDRLNMLL----DRL----------------------------THELEQMGYFLLLEDGT----LLSAQDGVFRTNCIDCLDRTNVVQSMIAKRVLNDVLSRLQILRKIEDHPSVEEHFKRIWADNADIISIQYSGTGALKTDFTRTGKRTKLGAMKDGLNSLTRYYKNNFTDGYRQDSLDLFLGRY 502