EWM23644.1, cds7040 (CDS) Nannochloropsis gaditana

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|585104796|gb|EWM23644.1| (trna pseudouridine synthase mitochondrial [Nannochloropsis gaditana])

HSP 1 Score: 1518.06 bits (3929), Expect = 0.000e+0
Identity = 740/740 (100.00%), Postives = 740/740 (100.00%), Query Frame = 0

Query:    1 MSRGQGGRWRGRNDGASRGADRAWGRGGRGWGRHGKRGRGGWKGDSTNKRKSDESAAEKGGPKRQCFYEDRAPHSGSYAAKQRHENSEDGQDLLGNKKKVAMIIGYCGTDFQGMQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKLHVKMKSEENVDENRKEEEENAEGTGKGGANESLHAGKEGEEGWSSMEIVKEGGPPMGAQEDETSDKVKSKCSESAGAGASATGEGGEVSAPVGKVEGREEGGHAPGAVSPSEADGPENISHENTSGGTIAAPFTTLSTSKGDGESEGRPDALSQEPPHRPPPAPDALAAALEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKKKGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWMYHCRQKYEMGELDAFAARGLTVAEQRSEAPKKQVMAEMMQEREEAARGKRLEQQEGACDEQGKEEEKLLVETDQTELEHENIVHGTEDLEGQVANHQNK 740
            MSRGQGGRWRGRNDGASRGADRAWGRGGRGWGRHGKRGRGGWKGDSTNKRKSDESAAEKGGPKRQCFYEDRAPHSGSYAAKQRHENSEDGQDLLGNKKKVAMIIGYCGTDFQGMQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKLHVKMKSEENVDENRKEEEENAEGTGKGGANESLHAGKEGEEGWSSMEIVKEGGPPMGAQEDETSDKVKSKCSESAGAGASATGEGGEVSAPVGKVEGREEGGHAPGAVSPSEADGPENISHENTSGGTIAAPFTTLSTSKGDGESEGRPDALSQEPPHRPPPAPDALAAALEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKKKGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWMYHCRQKYEMGELDAFAARGLTVAEQRSEAPKKQVMAEMMQEREEAARGKRLEQQEGACDEQGKEEEKLLVETDQTELEHENIVHGTEDLEGQVANHQNK
Sbjct:    1 MSRGQGGRWRGRNDGASRGADRAWGRGGRGWGRHGKRGRGGWKGDSTNKRKSDESAAEKGGPKRQCFYEDRAPHSGSYAAKQRHENSEDGQDLLGNKKKVAMIIGYCGTDFQGMQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKLHVKMKSEENVDENRKEEEENAEGTGKGGANESLHAGKEGEEGWSSMEIVKEGGPPMGAQEDETSDKVKSKCSESAGAGASATGEGGEVSAPVGKVEGREEGGHAPGAVSPSEADGPENISHENTSGGTIAAPFTTLSTSKGDGESEGRPDALSQEPPHRPPPAPDALAAALEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKKKGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWMYHCRQKYEMGELDAFAARGLTVAEQRSEAPKKQVMAEMMQEREEAARGKRLEQQEGACDEQGKEEEKLLVETDQTELEHENIVHGTEDLEGQVANHQNK 740          

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|553193884|ref|XP_005855852.1| (trna pseudouridine synthase, partial [Nannochloropsis gaditana CCMP526] >gi|422293209|gb|EKU20509.1| trna pseudouridine synthase, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 759.599 bits (1960), Expect = 0.000e+0
Identity = 371/376 (98.67%), Postives = 373/376 (99.20%), Query Frame = 0

Query:    8 RWRGRNDGASRGADRAWGRGGRGWGRHGKRGRGGWKGDSTNKRKSDESAAEKGGPKRQCFYEDRAPHSGSYAAKQRHENSEDGQDLLGNKKKVAMIIGYCGTDFQGMQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKLHVKMKSEENVDENRKEEEENAEGTGKGGANESLHAGKEGEEGWSSMEIVKEGGPPMGAQEDETSDKVKSKCSESAGAGASATGEGGEVSAPVGKVEGREEGGHAPGAVSPSEADGPENISHENTSGGTIAAPFTTLSTSKGDGESEGRPDALSQEPPHRPPPAPDALAAALEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYW 383
            RWRGR DGASRG DRAWGRGGRGWGRHGKRGRGGWKGDSTNKRKSDESAAE+GGPKRQCFYEDRAPHSGSYAAKQRHEN+EDGQDLLGNKKKVAMIIGYCGTDFQGMQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKLHVKMKSEENVDENRKEEEENAEGTGKGGANESLHAGKEGEEGWSSMEIVKEGGPPMGAQEDETSDKVKSKCSESAGAGASATGEGGEVSAPVGKVEGREEGGHAPGAVSPSEA GPENISHENTSGGTIAAPFTTLSTSKGDGESEGRPDALSQEPPHRPPPAPDALAAALEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYW
Sbjct:    1 RWRGRYDGASRGVDRAWGRGGRGWGRHGKRGRGGWKGDSTNKRKSDESAAEEGGPKRQCFYEDRAPHSGSYAAKQRHENAEDGQDLLGNKKKVAMIIGYCGTDFQGMQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKLHVKMKSEENVDENRKEEEENAEGTGKGGANESLHAGKEGEEGWSSMEIVKEGGPPMGAQEDETSDKVKSKCSESAGAGASATGEGGEVSAPVGKVEGREEGGHAPGAVSPSEAGGPENISHENTSGGTIAAPFTTLSTSKGDGESEGRPDALSQEPPHRPPPAPDALAAALEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYW 376          

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|1026951761|ref|XP_016611550.1| (tRNA pseudouridine synthase A [Spizellomyces punctatus DAOM BR117] >gi|907098137|gb|KND03511.1| tRNA pseudouridine synthase A [Spizellomyces punctatus DAOM BR117])

HSP 1 Score: 170.629 bits (431), Expect = 6.773e-42
Identity = 89/242 (36.78%), Postives = 131/242 (54.13%), Query Frame = 0

Query:  349 INRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKKKGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDW 590
            IN  LP  IRI G +R  R F+AKN C  R Y Y+LPT++L  V+     L                G+  G +   L++   +L    Q      R +R    QL+ L   L++Y GTH FHNF++   F   S  RYI++ E  DP++ G       G+EWV   + GQ+F+LHQIRKM+ +AIM+ R   PA+L+E  +++  L +P AP+ GL LDK  F +YNQ+ +  G+  ++D+
Sbjct:  209 INSHLPEQIRIWGFVRTNRSFHAKNACDARIYEYFLPTYVLQAVDPRLYPLSAV-------------GKKAGIKDAQLSFDYVELPKTEQKDLENKRSFRIEPEQLDLLRSILKEYEGTHNFHNFTVNKRFEDRSANRYIISFEASDPIIRG-------GIEWVKCKVLGQSFMLHQIRKMIGLAIMMVRTGTPASLVERTFENEKLNIPKAPALGLLLDKTLFRMYNQKRSS-GEDDAVDF 429          

HSP 2 Score: 89.3521 bits (220), Expect = 6.882e-15
Identity = 48/110 (43.64%), Postives = 64/110 (58.18%), Query Frame = 0

Query:   97 KKKVAMIIGYCGTDFQGMQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKLHVKMKSEENVDENRKEEEENAEGTGKGGANESLHA 206
            K+KVA+++GYCGT + GMQ+NPG  TIE EL KA+  AG + + N     KI + R+ RTDKGVHAA QV  C L + ++  + V +      E     G    N S HA
Sbjct:  124 KRKVALLMGYCGTGYNGMQINPGVETIELELHKALAAAGAVSKDNAMDPHKIAFMRAARTDKGVHAAGQV--CSLKMIIEDPDIVTKINSHLPEQIRIWGFVRTNRSFHA 231          

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|1023193204|gb|KZS90778.1| (tRNA pseudouridine synthase [Sistotremastrum niveocremeum HHB9708])

HSP 1 Score: 167.162 bits (422), Expect = 1.340e-40
Identity = 97/311 (31.19%), Postives = 153/311 (49.20%), Query Frame = 0

Query:  332 PPAPDALAAALEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKK-KGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWM 641
            P  PD +A         IN +LPP IR+ G +R    FNA+  C  R+Y Y+ P++L    +    +     +   K + G    G  G A  +   +    L +  +   +R R +R SS+ +ER    + KY+G+H FHNF++   ++  S +R + A+E+ DPVV G         EW+ V L GQ+F+LHQ RKMV VA+M+ R S P +LI + Y    +T+P AP+ GL L+   F  YN++   +      D   E+ +  +    P +     R +I  F +  I  ++   ED+  VF  W+
Sbjct:  161 PEVPDLVAR--------INEELPPEIRLWGFVRTQNPFNARVSCDSRKYTYFFPSYLFIPPKPDSGFARALQETNVKLRAGAETSGVDGAADDVHPFWKDSSLDSSPEEDLARKRTWRCSSQDVERFRTAVNKYLGSHNFHNFTVGREYKDPSNRRVMKAIEVSDPVVYG-------NTEWISVMLHGQSFMLHQ-RKMVCVAVMMCRTSTPPSLIPELYGQRKVTVPKAPALGLLLEYPVFENYNRKVEDLNGKIPKDANPEQLDAIR----PIIDFEQFRPQIDAFKQHYIYDNMRTHEDREGVFDGWI 451          

HSP 2 Score: 65.4698 bits (158), Expect = 1.964e-7
Identity = 52/159 (32.70%), Postives = 68/159 (42.77%), Query Frame = 0

Query:   22 RAWGRGGRGWGRHGKRGRG-------GWKGDSTNKRKSDESAAEKGGPKRQCFYEDRAPHSGSYAAKQRHENSEDGQDLLGNKKKVAMIIGYCGTDFQGMQM--NPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKL 171
            RA    G    RH K+ R             STN +      A   G +R    +D  P  G        E+ E  Q L   K+  A++IG+ GT + GMQ   N G  TIE  L  A+  AG + E N     K+   R+ RTD GVHAA  VV  ++
Sbjct:    8 RALDADGAASARHAKKSRTQDPTENPSDPTSSTNGKSGKRKLARNAGRRRGTRADDLPPQDG--------EDGEKKQRL--PKRACALLIGFSGTGYFGMQSQRNAGLKTIEGTLFDALVRAGAVSEDNADDPVKVNLQRAARTDAGVHAAGNVVSIRM 156          

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|1023238413|gb|KZT34347.1| (pseudouridine synthase [Sistotremastrum suecicum HHB10207 ss-3])

HSP 1 Score: 166.007 bits (419), Expect = 2.907e-40
Identity = 96/311 (30.87%), Postives = 153/311 (49.20%), Query Frame = 0

Query:  332 PPAPDALAAALEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKK-KGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWM 641
            P  PD +A         IN +LPP IR+ G +R    FNA+  C  R+Y Y+ P++L    +    +     +   K + G    G  G A  +   +    L +  +   +R R +R S + +ER    + KY+G+H FHNF++   ++  S +R + A+E+ DPVV G+        EW+ V L GQ+F+LHQ RKMV VA+M+ R S P +LI + Y    +T+P AP+ GL L+   F  YN++   +      D   E+ +  +    P +     R +I  F +  I  ++   ED+  VF  W+
Sbjct:  162 PDVPDLVAK--------INEELPPEIRLWGFVRTQNSFNARVSCDSRKYTYFFPSYLFIPPKPDSGFAKALQETNVKLRAGAETSGVDGAADEVHPFWKDSSLDSSPEEDLARKRTWRCSLQDVERFRTAVNKYLGSHNFHNFTVGREYKDPSNRRVMKAIEVSDPVVYGD-------TEWISVMLHGQSFMLHQ-RKMVCVAVMMCRTSTPPSLIPELYGQRKVTVPKAPALGLLLEYPVFENYNRKVEDLNGKIPKDANPEQLDAIR----PIIDFEQFRPQIDAFKQHYIYDNMRTHEDREGVFDGWI 452          

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|673014094|ref|XP_008861008.1| (tRNA pseudouridine(38-40) synthase [Aphanomyces invadans] >gi|574482606|gb|ETW09597.1| tRNA pseudouridine(38-40) synthase [Aphanomyces invadans])

HSP 1 Score: 160.229 bits (404), Expect = 7.068e-39
Identity = 101/300 (33.67%), Postives = 144/300 (48.00%), Query Frame = 0

Query:  342 LEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKKKGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWM 641
            L   R  +N  LPP I++  I+  T+ FN+KN C RR Y Y  PTF+ A     G   D      K     G+   A   +  S+    P+  A+++ F       R  +  L +L  TL  +VGTH FHN++ ++   S    RYI++ E  DP V         G EW+ + + GQ+FLLH IRKM+   + V RGS PAT I+ A++   + LP APS GLYL KA F +YN +      + + D      E D+             +RI  F E  I   I+ RE++  VF  W+
Sbjct:  152 LNEFRNNVNSFLPPDIQVLDIVAVTKNFNSKNSCDRRTYEYLTPTFVFA---PKGVMTD------KPATASGQWTAATYDEFNSIKV-DPEAWAMQKEF-------RLDATALAKLRETLAIFVGTHNFHNYTSKLPPYSPQCNRYILSFEADDPFV-------KNGTEWIRLRVVGQSFLLHHIRKMIGTTMDVLRGSTPATTIKTAFEQTKMDLPKAPSAGLYLAKAHFEVYNDKNENHRPSLAFD------EPDQ------------LDRIEAFKEAFIFPEIMEREEQDAVFPLWL 409          

HSP 2 Score: 108.997 bits (271), Expect = 1.516e-21
Identity = 52/100 (52.00%), Postives = 71/100 (71.00%), Query Frame = 0

Query:   74 HSGSYAAKQRHENSEDGQDLLGNKKKVAMIIGYCGTDFQGMQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKLHV 173
            HSGSYAA+    ++ D +     KK+  +++ YCG ++ GMQ+N G  TIEAELE+A+YEAG I   N+G +QKIGW+R+ RTDKGVHAA Q+V  KL +
Sbjct:   49 HSGSYAAQLDPADTTD-KCCAAPKKRYGLLLAYCGKNYSGMQVNIGVKTIEAELERALYEAGGIARCNYGMIQKIGWNRAARTDKGVHAAGQLVSMKLCI 147          

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|751183405|gb|KIL69344.1| (hypothetical protein M378DRAFT_70083 [Amanita muscaria Koide BX008])

HSP 1 Score: 160.614 bits (405), Expect = 2.075e-38
Identity = 107/362 (29.56%), Postives = 173/362 (47.79%), Query Frame = 0

Query:  349 INRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKKKGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWMYHCRQKYEMGELDAFAARGLTVAEQRSEAPKKQVMAEMMQEREEAARGKRLEQQEG-ACDEQGKEEEKL 709
            IN  LPP IR+ G +RA   FNA+  C  R+Y Y+ P+++L   +  G+   G  Q  K  G G           +   + +   S+ ++   +R R +R +  Q+ RL   + K+ GTH FHNF++   F+  S +R++  +E+ DPVV G+        EW+ V   GQ+F+LHQ RKM+   +M  R  AP+ +I++ Y   ++ +P  PS GL L++  F  YN R   I            +  D   R P +   + R++I +F ++ I  ++   ED+T +F  W+           +D +A   L     +   P+  V+ E   +R+   R KR     G A   + K EEKL
Sbjct:  169 INGLLPPEIRLWGFVRAQNSFNARIACDSRKYTYYFPSYILLPPKP-GS---GFDQASKHHGYGSDDSPLHTDVLLDNEFWKGHESSSKEEDLARKRAWRVNPSQVARLREIVSKFEGTHNFHNFTVGREFKDRSNQRHMKKIEVVDPVVYGD-------TEWISVLFHGQSFMLHQQRKMMTALVMTYRTGAPSDIIDELYSPRIVLVPKMPSLGLLLEEPVFESYNGRMAIINDKL--------DPSDPAYR-PPIDFDLYRDQIIKFKDDFIYKNMREVEDQTGLFDAWIRM---------VDTYAGNDLLYFNSKGTVPEAAVI-EKGVKRDNPFREKRKFNMTGFAATTEDKSEEKL 500          

HSP 2 Score: 65.855 bits (159), Expect = 1.571e-7
Identity = 31/75 (41.33%), Postives = 45/75 (60.00%), Query Frame = 0

Query:   97 KKKVAMIIGYCGTDFQGMQMNPGAI-TIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCK 170
            K++ A++IG+CGT + GMQ  P    TIE  L  A+ +AG + + N     K+  +R+ RTD GVHAA  +V  K
Sbjct:   83 KRQCALLIGFCGTGYSGMQFQPNVPRTIEGVLFDALVKAGAVSQDNADDPVKVNLARAARTDAGVHAAGNIVSIK 157          

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|673014096|ref|XP_008861009.1| (tRNA pseudouridine(38-40) synthase, variant [Aphanomyces invadans] >gi|574482607|gb|ETW09598.1| tRNA pseudouridine(38-40) synthase, variant [Aphanomyces invadans])

HSP 1 Score: 157.532 bits (397), Expect = 6.151e-38
Identity = 101/300 (33.67%), Postives = 144/300 (48.00%), Query Frame = 0

Query:  342 LEATRMAINRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKKKGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWM 641
            L   R  +N  LPP I++  I+  T+ FN+KN C RR Y Y  PTF+ A     G   D      K     G+   A   +  S+    P+  A+++ F       R  +  L +L  TL  +VGTH FHN++ ++   S    RYI++ E  DP V         G EW+ + + GQ+FLLH IRKM+   + V RGS PAT I+ A++   + LP APS GLYL KA F +YN +      + + D      E D+             +RI  F E  I   I+ RE++  VF  W+
Sbjct:  139 LNEFRNNVNSFLPPDIQVLDIVAVTKNFNSKNSCDRRTYEYLTPTFVFA---PKGVMTD------KPATASGQWTAATYDEFNSIKV-DPEAWAMQKEF-------RLDATALAKLRETLAIFVGTHNFHNYTSKLPPYSPQCNRYILSFEADDPFV-------KNGTEWIRLRVVGQSFLLHHIRKMIGTTMDVLRGSTPATTIKTAFEQTKMDLPKAPSAGLYLAKAHFEVYNDKNENHRPSLAFD------EPDQ------------LDRIEAFKEAFIFPEIMEREEQDAVFPLWL 396          

HSP 2 Score: 80.4925 bits (197), Expect = 2.926e-12
Identity = 42/80 (52.50%), Postives = 51/80 (63.75%), Query Frame = 0

Query:   98 KKVAMIIGYCGTDFQG---MQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKLHVK 174
            K   M   YC  D  G      N G  TIEAELE+A+YEAG I   N+G +QKIGW+R+ RTDKGVHAA Q+V  KL ++
Sbjct:   56 KCTYMHASYCLCDCCGHTPCHSNIGVKTIEAELERALYEAGGIARCNYGMIQKIGWNRAARTDKGVHAAGQLVSMKLCIE 135          

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|1021809351|gb|KZP01602.1| (pseudouridine synthase [Calocera viscosa TUFC12733])

HSP 1 Score: 157.918 bits (398), Expect = 2.691e-37
Identity = 92/294 (31.29%), Postives = 149/294 (50.68%), Query Frame = 0

Query:  349 INRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDGCAQQKKKKGGGGKRGEA-GGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWM 641
            +N  LPP +R+ G +R    FNA+  C  R Y Y  P+++L   +  G+ +   AQ    K    +   A  GA      +P   +  L +    R R +R     ++RL   ++ + GTH F+N+++   F+  +VKRY+M++E+ DP VVG+       +EW+ V   GQ+F+LHQIRKM+++AI+  R + P +LI ++Y    + +P AP+ GL L++  F  YN +   +G     D   +     +G     +     RE I RF  E I   + +RE +T  F  WM
Sbjct:  212 VNTHLPPEVRLWGTVRTANNFNARMSCDSRVYEYLFPSYILIPPKP-GSKM--AAQMASDKPVSDEALAALAGATKTGYMHPFWAVEDLGEPDLERKRRWRVGEESIKRLRELVKAFEGTHNFYNYTVGKDFKDKAVKRYMMSIEVRDPQVVGD-------MEWISVKFHGQSFMLHQIRKMISMAILACRAACPPSLILESYGPTRIRIPKAPALGLLLEEPQFGAYNAQMV-VGNRKLDDLRAKGRAVPEGDYREPIDFEPYREEIARFKLEMIYEKLRQREKETGTFDLWM 494          

HSP 2 Score: 81.2629 bits (199), Expect = 2.255e-12
Identity = 37/75 (49.33%), Postives = 50/75 (66.67%), Query Frame = 0

Query:   97 KKKVAMIIGYCGTDFQGMQMNPGAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKL 171
            KKKVA+++G+CGT   GMQ NP A TIE  L +A+ +AGC+   N     K+G +R+ RTD GVHAA  +V  K+
Sbjct:  124 KKKVALLVGFCGTGSHGMQYNPPARTIEGILFEALIKAGCVSADNSDDPNKVGLTRAARTDAGVHAAGNIVSMKI 198          

BLAST of EWM23644.1 vs. NCBI_GenBank
Match: gi|749898018|gb|KIJ65635.1| (hypothetical protein HYDPIDRAFT_88408 [Hydnomerulius pinastri MD-312])

HSP 1 Score: 155.606 bits (392), Expect = 1.360e-36
Identity = 95/316 (30.06%), Postives = 152/316 (48.10%), Query Frame = 0

Query:  349 INRQLPPSIRIHGILRATRGFNAKNDCTRRRYCYWLPTFLLAEVEEVGAWLDG--CAQQKKKKGGGGKRGEAGGAQGISLNYPQPDLSALRQCFRSRLRVYRASSRQLERLGRTLRKYVGTHKFHNFSIRMSFRSGSVKRYIMAVEMGDPVVVGEDGGEDKGLEWVCVTLTGQAFLLHQIRKMVAVAIMVSRGSAPATLIEDAYQDVMLTLPMAPSEGLYLDKASFALYNQRATQIGQAASLDWEGEEEEEDKGGRAPSLSVGVLRERIRRFLEEEIVGHILRREDKTLVFTKWMYHCRQKYEMGELDAFAARGLT 662
            +N +LPP IR+ G +R   GFNA+  C  R+Y Y+ P++LL   +   A++     A             +   A+  S   PQ DL+  RQ        +RAS   +ERL  T++K+ GTH FHNF++   F   S +R ++  ++ DPVV G+        EW+ V   GQ+F+LHQ RKM++  ++  R   P+ +I++ Y   M+ +P  P+ GL L+   F  YN R + + +    D       E+       +     RE I  F ++ I   +   ED+  +F  W+ +    YE  +L  F  RG+ 
Sbjct:  211 VNAELPPEIRVWGKVRTQNGFNARLSCDSRKYTYFFPSYLLIPPKPRSAFVRAQQAAASSPSNTPPHPFWDVADAESSS---PQEDLARKRQ--------WRASPEDVERLRATIKKFEGTHNFHNFTVGRDFSDRSNQRTMIKFQIADPVVYGD-------TEWISVLFHGQSFMLHQ-RKMMSALVLSCRTGTPSEIIDELYGPRMVLVPKMPALGLLLEYPIFQSYNNRISSVNEREKWDSSNPFFREN-------IDFEQYREAIDDFKQKFIYDGMRATEDQHGIFDAWIRYV-DNYEGNDLLYFNPRGII 499          

HSP 2 Score: 65.0846 bits (157), Expect = 3.378e-7
Identity = 34/78 (43.59%), Postives = 44/78 (56.41%), Query Frame = 0

Query:   97 KKKVAMIIGYCGTDFQGMQMNP---GAITIEAELEKAMYEAGCIKEANFGSLQKIGWSRSGRTDKGVHAAAQVVGCKL 171
            K+  A++IG+CGT   GMQ+ P   G  TIE  L  A+  AG + + N     K+   R+ RTD GVHAA  VV  KL
Sbjct:  120 KRMTALLIGFCGTGCSGMQIQPREAGLRTIEGVLFDALVRAGAVSQDNSDDPVKVNLGRAARTDAGVHAAGNVVSMKL 197