EWM24288.1, cds6416 (CDS) Nannochloropsis gaditana

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|585105679|gb|EWM24288.1| (dna gyrase subunit a [Nannochloropsis gaditana])

HSP 1 Score: 2035.38 bits (5272), Expect = 0.000e+0
Identity = 1001/1001 (100.00%), Postives = 1001/1001 (100.00%), Query Frame = 0

Query:    1 MFAASVSAVLKSARRGGRFGFHLQPWPPSFVPTLALNPRPVPERGSRWTLQAARGLSKFSRDGGGGARSMRNKKTRLSVKLSSRQGDGSDDDGIPREPEILRDFGGGNGEKMGGESQSISAAGGDSGYGDKVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRFASADLKPTGRTSRGCSAIKLRKTDEMVDITVLRPDAAVDVDGPGDCGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSGDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADSDDDDEDGSLANKADAVMKDLRYENEAAVVMSIGSQTGVVDA 1001
            MFAASVSAVLKSARRGGRFGFHLQPWPPSFVPTLALNPRPVPERGSRWTLQAARGLSKFSRDGGGGARSMRNKKTRLSVKLSSRQGDGSDDDGIPREPEILRDFGGGNGEKMGGESQSISAAGGDSGYGDKVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRFASADLKPTGRTSRGCSAIKLRKTDEMVDITVLRPDAAVDVDGPGDCGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSGDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADSDDDDEDGSLANKADAVMKDLRYENEAAVVMSIGSQTGVVDA
Sbjct:    1 MFAASVSAVLKSARRGGRFGFHLQPWPPSFVPTLALNPRPVPERGSRWTLQAARGLSKFSRDGGGGARSMRNKKTRLSVKLSSRQGDGSDDDGIPREPEILRDFGGGNGEKMGGESQSISAAGGDSGYGDKVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRFASADLKPTGRTSRGCSAIKLRKTDEMVDITVLRPDAAVDVDGPGDCGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSGDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADSDDDDEDGSLANKADAVMKDLRYENEAAVVMSIGSQTGVVDA 1001          

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|299116769|emb|CBN74882.1| (DNA gyrase subunit A [Ectocarpus siliculosus])

HSP 1 Score: 1051.58 bits (2718), Expect = 0.000e+0
Identity = 510/837 (60.93%), Postives = 641/837 (76.58%), Query Frame = 0

Query:  129 GDKVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQD-DLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRFAS--ADLKPTGRTSRGCSAIKLRKTDEMVDITVLRPDAAVDVDGPGDCGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSGDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADSDD 962
            GD+V+   L  E+ +SFMQYAMS ILGRALPD RDGLKPVHRRIL+AM+ LNL PE ++RKCARVVGEVLGKYHPHGD +VYDALVRMAQDFVMSAPL+ GHGNFGS+D+DPAAAMRYTECKLS +A+D LL DINLDTVD   NFDGNE EP+VLP+R+P+LL+NGATGIAVGMATN+PPHNLGEV+  T+ALI +P + DD+L RL+PAPDFPTGG IMGL G RKLYT  +G + +RA TH+E I    GR  RNA+VVTELPYQVNK+SLL+++A+MVN+KK+EGISDLRDESDR+G+RVV+ELKRDA P VVENNLFKKT LQ+SF GN LAL +DG +P R +LR ++QSF+DFRF T+RRRT  EL K+ +R+H+V+G+++AL  ID V+ELVR + DT  A+  LM   Y +S+ QA AILAL LGRLT++E+ KL+AE E L+  +E  + +M +D KV D++ +EL E + KHAVPRR+ I+   G L E DLLAND SV++VT AGYIKRMPL++F  Q RGTRGKAGA+MS D D+V HFF C DHD ++F+S+KGVA+GIRAFQVPV SR +KGVP+PQVLP++ D+ ++SV+ + + F+ DE+LVLLT++G+IK+TP+ AFK  +SRGLI ISLGE DTL W +RC D++ I+I +++GY+ RF +   DL+P+GRTSRG                                     G  LL +TT GYGKRV+ + FK  RRGG GVIA+K +    D+LAC R    D E++LST +GTIVRQR  AIA QSRTATGV VQ+LD  + IK VA++P  S+D
Sbjct:  162 GDQVVNVGLEAEIKTSFMQYAMSIILGRALPDVRDGLKPVHRRILYAMYGLNLNPEGTHRKCARVVGEVLGKYHPHGDTAVYDALVRMAQDFVMSAPLIQGHGNFGSIDNDPAAAMRYTECKLSSVAKDTLLTDINLDTVDMMTNFDGNEKEPVVLPSRLPLLLINGATGIAVGMATNIPPHNLGEVVAATIALIRNPGIPDDQLFRLLPAPDFPTGGRIMGLAGARKLYTTSNGGVTLRATTHIEVIE-AKGRTKRNAVVVTELPYQVNKSSLLQRMAEMVNDKKLEGISDLRDESDRDGVRVVVELKRDANPQVVENNLFKKTQLQTSFSGNMLALGSDGTKPHRFSLRAAIQSFVDFRFTTVRRRTAFELGKVRAREHIVQGMLQALGMIDAVIELVRASTDTPMARDGLMAEPYGLSQEQADAILALRLGRLTSMEEGKLKAEAEDLKAAMERLEGVMNEDSKVMDIIVEELTEAKNKHAVPRRTMIKPDEGELHEEDLLANDNSVVVVTAAGYIKRMPLEEFTAQNRGTRGKAGAKMSSDHDVVQHFFTCQDHDTILFVSDKGVAYGIRAFQVPVGSRTAKGVPVPQVLPIAGDEQIASVLAV-DDFREDEYLVLLTQKGYIKRTPLAAFKSTSSRGLIIISLGEDDTLRWVKRCCDENGIVIGSKQGYATRFLANQKDLRPSGRTSRGVK---------------------------------EEGHSLLVLTTRGYGKRVALNEFKTTRRGGKGVIALKLRQKLEDTLACFRIVGADDEIMLSTAQGTIVRQRAGAIARQSRTATGVIVQKLDANDGIKDVALVPEISED 963          

HSP 2 Score: 64.6994 bits (156), Expect = 7.750e-7
Identity = 64/241 (26.56%), Postives = 108/241 (44.81%), Query Frame = 0

Query:  629 LDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPV--ASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFK--KLTSRGLIAISLGEG--DTLGWARRCTDQDDILISTRKGYSMRFASADLKPTGRTSRGCSAIKLRKTDEMVDITV---LRPDAAVDVDGPG-----DCGSGS 855
            +D    D  ++++T+ GYIKR PL  F+     +RG     + +DD +     C D + +V  S++G A    A Q  +  + R S+GV                        +    L++LT +G+ K+  ++ FK  +   +G+IA+ L +   DTL   R     D+I++ST +G  +R  +  +    RT+ G    KL   D + D+ +   +  DAA     PG     D G+GS
Sbjct:  768 VDDFREDEYLVLLTQKGYIKRTPLAAFKST--SSRGLIIISLGEDDTLRWVKRCCDENGIVIGSKQGYATRFLANQKDLRPSGRTSRGVK-----------------------EEGHSLLVLTTRGYGKRVALNEFKTTRRGGKGVIALKLRQKLEDTLACFRIVGADDEIMLSTAQGTIVRQRAGAIARQSRTATGVIVQKLDANDGIKDVALVPEISEDAAAATGRPGASSGDDVGAGS 983          

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|873234992|emb|CEM01585.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 981.089 bits (2535), Expect = 0.000e+0
Identity = 516/890 (57.98%), Postives = 678/890 (76.18%), Query Frame = 0

Query:  132 VLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPM----GGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRFAS--ADLKPTGRTSRGCSAIKLRKTDEMVDITVLRPDAAVDVDGPG---DCGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFK----------AGS-----------GDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLP-ADSDDDDEDGSLANKADAVMKDLRYENEAAVVM 990
            V+  EL  ELS+SFM YAMS ILGRALPDARDGLKPVHRRIL+AM  LNL PESS+RKCARVVGEVLGKYHPHGD +VYDALVRMAQDFVMS PLV GHGNFGSVD DP AAMRYTECKL+ L+RDALL D+++DTVDF  NFDGNEVEP +LPA++P LLLNGATGIAVGMATN+PPH LGE++D  +ALI++PSLSD++L  LIPAPDFPTG  I+GLEG RKLY  G+G++VMRA+TH+E I      GG    R AIVVTELPY VNK+ LLE++A MVNEKK+EGI+DLRDESDR+G R+VIELK+DA  +VV+NNLFKKT LQ+SF GN L+L +DG+QP RLTLR +LQ ++DFRF+TIRRRT  EL + + R HVVEGL++AL +IDDV+++VR+AKD   A+  L GS +D+SE QA+A+L LTL RLTALE+ KL+ E E+LR +I + +A+MR+D KV+ +M+ EL+E++ K+   RRS I   A  LTE DL+ NDRSV++VT +GY+KR+P+++F+ Q RGTRGK GA++S DD V HFFACNDHD ++F+S +GVAF ++A+QVP++SR ++GVPLPQVLP+++DD ++SV+P+ + F+ DE+LVLLTK+G+IKKTP+ AF  +++RGL  I+L   D L + RRCTD D ++I+TR GY++RF +    L+PT RTSRG  ++K+R  D++ D+ +L          PG   D G G+N  +LLAVT  G GKR+    F++Q RGG+GVIA KFK          +G+           GD+++CMR C  + E++LST  GT++RQ+   I+LQ RTATGV VQRL +G+ ++SV+++P A+ DD     +  +  D+ ++D   +NE   V+
Sbjct:   25 VMDVELESELSASFMSYAMSIILGRALPDARDGLKPVHRRILWAMQGLNLSPESSFRKCARVVGEVLGKYHPHGDVAVYDALVRMAQDFVMSGPLVNGHGNFGSVDGDPPAAMRYTECKLTPLSRDALLADVSMDTVDFVANFDGNEVEPTILPAKLPFLLLNGATGIAVGMATNIPPHQLGELVDACIALIKNPSLSDERLFSLIPAPDFPTGALIVGLEGVRKLYKTGNGAVVMRARTHVEEIKKTNRKGGQLGSRTAIVVTELPYMVNKSVLLERMATMVNEKKLEGIADLRDESDRDGTRIVIELKKDARATVVQNNLFKKTQLQTSFAGNLLSLGDDGKQPVRLTLRSALQIWLDFRFRTIRRRTNFELGRRKQRAHVVEGLLKALAQIDDVIDVVRQAKDANQAREALQGSPFDLSEEQAEALLRLTLARLTALEEEKLKTELEELRARISELEALMREDSKVYHLMETELKELKRKYGGQRRSEIIHEAADLTEEDLIPNDRSVLIVTHSGYVKRLPVEEFDPQSRGTRGKLGAKISDDDSVAHFFACNDHDTLLFVSNRGVAFTVKAYQVPLSSRTARGVPLPQVLPIAADDRITSVLPITQ-FKEDEYLVLLTKKGWIKKTPLAAFANVSNRGLTLIALEPDDVLRFVRRCTDDDSVIIATRGGYAIRFRTDGEQLRPTSRTSRGVKSLKIRDGDQVADLDIL----------PGNEDDDGKGANKFFLLAVTENGCGKRIKPSHFRLQSRGGLGVIATKFKPQGKGNGGNTSGNKASSAEKKKLGGDAVSCMRVCRLNDEIMLSTAGGTVLRQKAGEISLQGRTATGVLVQRLPDGDEVQSVSVVPEAEQDDLIPPSATQSDTDSDVEDEVVDNEGVEVI 903          

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|1057114372|ref|WP_068510124.1| (DNA gyrase subunit A [Leptolyngbya sp. O-77] >gi|984537235|dbj|BAU42215.1| DNA gyrase subunit A [Leptolyngbya sp. O-77])

HSP 1 Score: 941.028 bits (2431), Expect = 0.000e+0
Identity = 458/851 (53.82%), Postives = 628/851 (73.80%), Query Frame = 0

Query:  130 DKVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRFAS--ADLKPTGRTSRGCSAIKLRKTDEMVDITVLRPDAAV-------DVDGPGDCG----SGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSGDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADSDDDDEDG 967
            D+++PT+L  E+S S+++YAMS I+GRALPDARDGLKPVHRRIL+AMH L L P+  +RKCARVVG+V+GKYHPHGD +VYDALVRMAQDF M +PLV GHGNFGSVD+DP AAMRYTEC+L  +  +ALL+DI  +TVDF PN+DG++ EPLVLPAR+P LLLNG++GIAVGMATN+PPHNLGE+LDG +ALI +P ++D +L+++IP PDFPTGG I+G  G R+ Y  G GSI MR    +ETI   G    R AI++TELPYQ NKA+L+EKIA+MVNE+K+EGI+D+RDESDR+G+R+VIEL+RDA P VV NNL+K+T LQ++F  N LAL N   +PQ L L+ESLQ F+DFR +TI RRT++EL K E RDH+++G + AL  +D+++ L+R A DT  AK  LM  +Y +SEAQA AIL + L RLTALE  K++AE ++L+ +I D + ++   E++  +++ E  E++A HA PRR+ I    G L ++DL+AN+R++++VTE GYIKRMP+  FE Q R TRGK+G RM +DD ++HF  CNDHD+++F S++GVA+ +RA+QVP  SR S+GVP+ Q+LP+  ++ ++SV+ + E F  D +LV+LT++GFIKKT + AF  + + GLIAISL +GD L W R  T  D I+I +RKG ++ F +    L+P GR +RG  A+ LR+ DE++ + +L P   V       D D  GD      S + G ++L +TT G GKRV  + F++Q+R GMG+ AI+F+  +GD LA +R  ++D E++L T +G I+RQ V AI+ QSR ATGVRVQRLDE + I  VA++P   + +D D 
Sbjct:   10 DRIVPTDLRTEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMHELGLAPDRPFRKCARVVGDVIGKYHPHGDTAVYDALVRMAQDFSMRSPLVDGHGNFGSVDNDPPAAMRYTECRLRAITTNALLQDIESETVDFVPNYDGSQQEPLVLPARIPQLLLNGSSGIAVGMATNIPPHNLGELLDGLVALIANPDMTDAELMQIIPGPDFPTGGIILGTSGIREAYATGRGSITMRGVATIETIERRGAPD-REAIIITELPYQTNKAALIEKIAEMVNERKLEGIADIRDESDRDGMRIVIELRRDAYPRVVLNNLYKQTPLQANFGVNTLALVNG--EPQLLGLKESLQVFLDFRIETITRRTRYELRKAEERDHILQGYLIALANLDEIIALIRGAADTPTAKQELM-ERYSLSEAQADAILQMQLRRLTALEAGKIQAEHDELQVKIADLRDILANRERILQIIRDEAVELKAAHATPRRTVIEPLDGELGDIDLIANERAIILVTEQGYIKRMPVSTFEAQNRATRGKSGTRMKEDDGIDHFLTCNDHDNILFFSDRGVAYCLRAYQVPTGSRTSRGVPIVQLLPIPVEEKITSVVAVTE-FTDDLYLVMLTQKGFIKKTQLSAFGNIRTNGLIAISLEDGDQLRWVRLTTADDSIIIGSRKGMTIHFRTDHEQLRPLGRPTRGVRAMSLREGDELISMDIL-PSQVVQTIAEGDDTDNDGDLAEEECSTAQGPWVLVITTGGLGKRVPVNQFRLQKRAGMGLRAIRFRK-AGDELAALRVVNEDDEMILVTSRGIIIRQVVNAISCQSRAATGVRVQRLDEDDAIADVALVPPSGEAEDPDA 853          

HSP 2 Score: 79.7221 bits (195), Expect = 1.938e-11
Identity = 70/248 (28.23%), Postives = 112/248 (45.16%), Query Frame = 0

Query:  745 LVLLTKQGFIKKTPVDAF--KKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKG--YSMRFASADLKPTG-RTSRGCSAIKL------RKTDEMVDITVLRPDAAVDVDGPGDCGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSGDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQ--SRTATGVRVQRLDEGNIIKSVAMLP-------ADSDDDDEDGSLANK 972
            ++L+T+QG+IK+ PV  F  +   +RG     + E D +     C D D+IL  + +G  Y +R       PTG RTSRG   ++L       K   +V +T    D                  YL+ +T  G+ K+    +F   R  G+  I+++     GD L  +R  + D  +++ ++KG  +  R     L+   R   GVR   L EG+ + S+ +LP       A+ DD D DG LA +
Sbjct:  514 IILVTEQGYIKRMPVSTFEAQNRATRGKSGTRMKEDDGIDHFLTCNDHDNILFFSDRGVAYCLRAYQV---PTGSRTSRGVPIVQLLPIPVEEKITSVVAVTEFTDDL-----------------YLVMLTQKGFIKKTQLSAFGNIRTNGLIAISLE----DGDQLRWVRLTTADDSIIIGSRKGMTIHFRTDHEQLRPLGRPTRGVRAMSLREGDELISMDILPSQVVQTIAEGDDTDNDGDLAEE 737          

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|763348317|ref|WP_044206581.1| (DNA gyrase subunit A [Coleofasciculus chthonoplastes])

HSP 1 Score: 931.784 bits (2407), Expect = 0.000e+0
Identity = 450/849 (53.00%), Postives = 619/849 (72.91%), Query Frame = 0

Query:  130 DKVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRFAS--ADLKPTGRTSRGCSAIKLRKTDEMVDITVL-------------------RPDAAVDVDGPGDCGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSGDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLP 957
            ++++PT L  E+S S+++YAMS I+GRALPDARDGLKPVHRRIL+AMH L L P+  +RKCARVVGEVLGKYHPHGD +VYDALVRMAQDF M +PL+ GHGNFGSVD+DP AAMRYTEC+L  LA D+LL+DI  +TVDF  NFDG++ EP+VLPARVP +LLNG++GIAVGMATN+PPHNLGE++DG +ALI +P +++ +L++ IPAPDFPTGG I+G  G +  YT G GSI MR    +ETI    GR  + AI+VTELPYQ NKA+L+EKIA+MVN+K++EGISD+RDESDR G+RVVIELKRDA P VV NNL+K+T LQS+F  N LAL   G +PQ LT+++ L+ F++FR +TI RRTQ+EL K E RDH+++GL+ ALD +D V+ ++R A DTA AK+ L+  Q+ +SE QA AIL + L RLTALE  K++AE E+L+ +I D Q ++    ++ ++++ E  +I+  H  PRR++I+   G L ++DL+AN+++V++VTE GYIKRMP+  F  Q R TRGKA ARM +DD V HF  C DHD ++F S++GV + +RA+Q+P ASR ++GVP+ Q+LP+  D+ ++SV+P+ E F  D +LV+LT++G+IKKT +DAF  + + GLIAISLGEGD L W R C  +D I+I + +G ++ F +    L+P GR +RG  A+KLR  DE++ + +L                     D + +V+G     +   G ++L VTT G GKRV    F++Q R GMG++ IKF+    D LA +R  ++  E+++ T +G I+RQ + AI+ QSR ATG+RVQRLDE + I +VA++P
Sbjct:    6 ERIVPTNLRSEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMHELGLTPDRPFRKCARVVGEVLGKYHPHGDTAVYDALVRMAQDFSMRSPLINGHGNFGSVDNDPPAAMRYTECRLQSLATDSLLRDIESETVDFLDNFDGSQQEPIVLPARVPQILLNGSSGIAVGMATNIPPHNLGELVDGLVALIHNPEITEKELMQYIPAPDFPTGGQILGRSGIKDAYTTGRGSITMRGVAQVETIE-HRGRPDKEAIIVTELPYQTNKAALIEKIAEMVNDKRIEGISDIRDESDRRGMRVVIELKRDAYPRVVLNNLYKQTPLQSNFGANMLALV--GNEPQLLTIKQFLEVFLEFRIETITRRTQYELRKAEERDHLLQGLLIALDNLDAVIAVIRAANDTATAKSQLI-EQFGLSEPQADAILQMQLRRLTALEAQKIQAEHEELQVRIADLQDILANRSRILEIIETEALQIKTTHDTPRRTAIQEVEGELDDIDLIANEKAVILVTEQGYIKRMPIHSFAAQARATRGKAAARMKEDDGVEHFLTCCDHDSLLFFSDRGVVYSLRAYQIPAASRTARGVPIVQMLPIPHDEKITSVVPVSE-FTDDVYLVMLTRKGYIKKTALDAFSNIRANGLIAISLGEGDQLRWVRLCRAEDSIIIGSSQGMAIHFKADHDQLRPLGRATRGVKAMKLRSKDELISMDILPAQVVERLIATANSEEVETEDDTSDEVNGEEVHETAGQGPWVLVVTTGGLGKRVPVSQFRLQNRAGMGLVTIKFR-HKKDKLAALRVVNEGDELMMVTMRGIIIRQALDAISPQSRMATGIRVQRLDEEDAIAAVALVP 848          

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|499369631|ref|WP_011057209.1| (DNA gyrase subunit A [Thermosynechococcus elongatus] >gi|22298912|ref|NP_682159.1| DNA gyrase subunit A [Thermosynechococcus elongatus BP-1] >gi|22295093|dbj|BAC08921.1| DNA gyrase A subunit [Thermosynechococcus elongatus BP-1])

HSP 1 Score: 930.628 bits (2404), Expect = 0.000e+0
Identity = 460/844 (54.50%), Postives = 620/844 (73.46%), Query Frame = 0

Query:  131 KVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRF-ASAD-LKPTGRTSRGCSAIKLRKTDEMVDITVLRPDAAVDVDGPGDCGSG-----------SNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSG-DSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADS 960
            +++PTEL  E+S S+++YAMS I+GRALPDARDGLKPVHRRIL+AM+ L L  +  +RKCARVVGEVLGKYHPHGD++VYDALVRMAQDF M  PL+ GHGNFGS+D+DP AAMRYTEC+L  LA +ALL+DI  +TVDF  NFDG++ EPLVLPAR+P LLLNGA+GIAVGMATN+PPHNLGE++DG +ALI  P +SD +L+R IP PDFPTGG I+G  G  + YT G GS+ +RA   +ET+    GRQ R AI++TELPYQ NKA+L+EKIA++VNEKK+EGI+DLRDESDR+GIRVVIELKRDA P VV NNL+K+T LQ +F  N LA+ N   +PQ LTL+ SL+ F+ FR + I RRT++ L K E RDH+++GL+ AL  +D V++L+R A DTA A+  LM + Y +SEAQA AIL + L RLTALE  K+  E  +L+ QI D++ ++   ++V +++++E+ E++AK A PRRS I    G ++++DL+AND+SV++VT+ GYIKRMP+D FE Q R  RG+ GA + +DD V HFF+CNDHD ++F S++G+ + + A+Q+P  SR ++G P+ Q+LP+  ++ ++SVI + E F  DE+LV+LT++GFIKKTP+ AF  + S GLIAISL EGD L W RR  +QD I+I +R+G ++ F AS D L+P GR +RG  ++ LR  DE+V + +L    A     P +  SG           S G ++L +TT GYGKRV    F++Q R GMG+ A KFKA S  D LA +R  +   E+++ T +G I+RQ+V  I+ QSR+ATGVR+QRLDE + I + A+LP  S
Sbjct:    9 RIIPTELREEISRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMYELGLTSDRPFRKCARVVGEVLGKYHPHGDSAVYDALVRMAQDFSMRHPLIEGHGNFGSIDNDPPAAMRYTECRLQALATEALLQDIEQETVDFVDNFDGSQQEPLVLPARIPQLLLNGASGIAVGMATNIPPHNLGELVDGLVALIHHPQMSDRELMRYIPGPDFPTGGHILGQGGIEEAYTTGRGSMTLRAVATIETLE-APGRQPREAIIITELPYQTNKAALMEKIAELVNEKKIEGIADLRDESDRDGIRVVIELKRDAHPRVVLNNLYKQTPLQVNFGANMLAIVNG--EPQLLTLKRSLEVFLRFREEAIARRTRYALRKAEERDHLLQGLLVALANLDAVIQLIRSASDTALARQQLMQT-YALSEAQADAILQMQLRRLTALEAEKIEREHAELQRQIADYRDILAHRQRVLEIIEKEVTELKAKFATPRRSRIVQADGEISDIDLIANDKSVILVTQQGYIKRMPVDTFEAQSRDGRGRKGAEIKEDDAVEHFFSCNDHDRILFFSDRGLVYALAAYQIPSGSRQARGTPIVQLLPIPREEKITSVIAVQE-FSEDEYLVMLTRKGFIKKTPLAAFSHIRSNGLIAISLEEGDQLRWVRRTREQDTIIIGSRQGMAIHFRASHDQLRPLGRATRGVKSMNLRPGDELVGMDILPAAIANRFATPSEDDSGEIEDSEETVAHSEGPWVLVITTNGYGKRVPVQQFRLQNRAGMGITATKFKAKSNEDQLAALRIVNAGDELMIVTSRGIIIRQKVMDISSQSRSATGVRLQRLDEDDAIVTAAVLPPGS 847          

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|1186534854|ref|WP_085435821.1| (DNA topoisomerase (ATP-hydrolyzing) subunit A [unicellular cyanobacterium SU2])

HSP 1 Score: 929.087 bits (2400), Expect = 0.000e+0
Identity = 447/851 (52.53%), Postives = 624/851 (73.33%), Query Frame = 0

Query:  130 DKVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRFASAD--LKPTGRTSRGCSAIKLRKTDEMVDITVLRPDAAVDV------------DGPGDCGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSGDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADSDDDDED 966
            D+++PT+L+ E+S S+++YAMS I+GRALPDARDGLKPVHRRIL+AM+ L L PE  +RKCARVVGEVLGKYHPHGD +VYDALVRMAQ F M  PL+ GHGNFGSVD+DP AAMRYTEC+L  L+ +ALL+DI  +TVDFA NFDG++ EP+VLPAR+P LLLNG++GIAVGMATN+PPHNLGE++DGT+ALI +P + + +L ++IP PDFPTGG I+G  G +  YT G GSI MR    +ET+    GR  R AI+VT+LPYQ NKA+L+E+IAD+VN+K++EGISD+RDESDR+G+RVVIELKRDA P VV NNL+K+T +QS+F  N LAL N   +PQ LTL++ LQ F+DFR +TI RRTQ+EL K + RDH+++GL+ AL  +D V+ L+R+A DT  A+  L+  Q+ +S  QA AIL + L RLTALE  K+ AE E+L+ +I D Q ++ + E++  ++K+EL +I++ HA PRR+ I    G + + DL+AN+++++++TE GYIKRMP+  FE Q R TRGKA A++ +DD+V HF  C DHD V+F S++GV +GI A+Q+P ASR ++GVP+ Q+LP+  D+ ++S++ + E F  D +L++LT+ G+IKKT + AF  + + GLIAISLGEGD L W R   ++D I+I TR+G ++ F + +  L+P GR++RG  A+KL+K DE + + +L       +                     + G ++LA+T  GYGKRV  + F++Q R GMGV AIKFK+ S D LA +   ++D E+++ + +G I+RQ V AI+LQSR ATGVRVQRLD  + I +VA++P  ++ ++ +
Sbjct:    6 DRIIPTDLSNEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMYELGLTPERPFRKCARVVGEVLGKYHPHGDTAVYDALVRMAQHFSMRNPLIEGHGNFGSVDNDPPAAMRYTECRLQSLSTNALLRDIEAETVDFADNFDGSQQEPIVLPARIPQLLLNGSSGIAVGMATNIPPHNLGELIDGTIALINEPEIEEVELRKIIPGPDFPTGGQILGRSGIKDAYTTGRGSITMRGVAQIETLEQ-RGRPDREAIIVTQLPYQTNKAALIERIADLVNDKRIEGISDIRDESDRDGMRVVIELKRDAYPRVVLNNLYKQTPIQSNFGANMLALVNG--EPQLLTLKDFLQVFLDFRIETITRRTQYELRKAQERDHLLQGLLIALSNLDAVIRLIRQAADTTTAREELV-EQFSLSVVQADAILQMQLRRLTALEADKIHAEHEELQAKIADLQDILARRERIETIIKEELEQIKSIHATPRRTEIIRDDGEIVDTDLIANEQAIILLTEQGYIKRMPVSTFEAQSRATRGKAAAKIKEDDVVEHFLTCCDHDQVLFFSDRGVVYGIPAYQIPAASRTARGVPIVQMLPIPKDEKITSIVAVDE-FSEDVYLIMLTRNGYIKKTALAAFSNIRANGLIAISLGEGDQLRWVRLAREEDSIIIGTRQGMAIHFHADNQQLRPLGRSTRGVKAMKLKKGDEFISMDILSSQVIAGMEDTEEEDEELLETEELTPEETTGGPWVLAITNGGYGKRVPVNKFRLQNRAGMGVKAIKFKSKS-DRLASLHIVNEDDELMIVSNRGIIIRQAVNAISLQSRMATGVRVQRLDSDDAIAAVALVPQAAEGEESN 850          

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|571029438|ref|WP_024124235.1| (DNA gyrase subunit A [Thermosynechococcus sp. NK55a] >gi|564737424|gb|AHB87822.1| DNA gyrase subunit A GyrA [Thermosynechococcus sp. NK55a])

HSP 1 Score: 928.702 bits (2399), Expect = 0.000e+0
Identity = 457/844 (54.15%), Postives = 619/844 (73.34%), Query Frame = 0

Query:  131 KVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRF-ASAD-LKPTGRTSRGCSAIKLRKTDEMVDITVLRP-----------DAAVDVDGPGDCGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGSG-DSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADS 960
            +++PTEL  E+S S+++YAMS I+GRALPDARDGLKPVHRRIL+AMH L L  +  +RKCARVVGEVLGKYHPHGD++VYDALVRMAQDF M  PL+ GHGNFGS+D+DP AAMRYTEC+L  LA +ALL+DI  +TVDF  NFDG++ EPLVLPAR+P LLLNG +GIAVGMATN+PPHNLGE++DG +ALI  P +SD +L+R IP PDFPTGG I+G  G  + YT G GSI +RA   +ET+    GRQ R AI+VTELPYQ NKA+L+EKIA++VNEKK+EGI+DLRDESDR+GIRVVIELKRDA P VV NNL+K+T LQ +F  N LA+ N   +PQ LTL+ SL+ F+ FR + I RRT++ L K E RDH+++GL+ AL  +D V++L+R A DTA A+  LM + Y +SEAQA AIL + L RLTALE  K+  E  +L+ QI D++ ++   ++V +++++E+ E++AK+A PRRS I    G +++ DL+AND+SV++VT+ GYIKRMP+D FE Q R  RG+ GA + +DD V HFF+CNDHD ++F S++G+ + + A+Q+P  SR ++G P+ Q+LP+  ++ ++SVI + E F  DE+LV+LT++GFIKKTP+ AF  + + GLIAISL EGD L W RR  ++D I+I +R+G ++ F AS D L+P GR +RG  ++ LR  DE+V + +L             D   + +   +    S G ++L +TT GYGKRV    F++Q R GMG+ A KFKA S  D LA +R  + + E+++ T +G I+RQ+V  I+ QSR+ATGVR+QRLDE + I + A+LP  S
Sbjct:    9 RIIPTELREEISRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMHELGLTSDRPFRKCARVVGEVLGKYHPHGDSAVYDALVRMAQDFSMRHPLIEGHGNFGSIDNDPPAAMRYTECRLQALATEALLQDIEQETVDFVDNFDGSQQEPLVLPARIPQLLLNGTSGIAVGMATNIPPHNLGELVDGLVALIHHPQISDRELMRYIPGPDFPTGGHILGQGGIEEAYTTGRGSITLRAVATIETLE-APGRQPREAIIVTELPYQTNKAALMEKIAELVNEKKIEGIADLRDESDRDGIRVVIELKRDAYPRVVLNNLYKQTPLQVNFGANMLAIVNG--EPQLLTLKRSLEVFLRFREEAIARRTRYALRKAEERDHLLQGLLVALANLDAVIQLIRSASDTALARQQLMQT-YALSEAQADAILQMQLRRLTALEAEKIEREHAELQRQIADYRDILAHRQRVLEIIEKEVTELKAKYATPRRSRIVQADGEISDTDLIANDKSVILVTQQGYIKRMPVDTFEAQSRDGRGRKGAEIKEDDAVEHFFSCNDHDRILFFSDRGLVYALPAYQIPSGSRQARGTPIVQLLPIPREEKITSVIAVQE-FSEDEYLVMLTRKGFIKKTPLAAFSNIRANGLIAISLEEGDQLRWVRRTREEDTIIIGSRQGMAIHFRASHDQLRPLGRATRGVKSMSLRPGDELVGMDILPAAIANRFAATPEDDRAESEDAEETVVHSEGPWVLVITTNGYGKRVPVQQFRLQNRAGMGITATKFKAKSNEDQLAALRIVNAEDELMIVTSRGIIIRQKVMDISSQSRSATGVRLQRLDEEDAIVTAAVLPPGS 847          

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|1121317970|ref|WP_073628109.1| (DNA topoisomerase (ATP-hydrolyzing) subunit A [Scytonema sp. HK-05] >gi|1115296955|gb|OKH59944.1| DNA gyrase subunit A [Scytonema sp. HK-05] >gi|1210123497|dbj|BAY42611.1| DNA gyrase subunit A [Scytonema sp. HK-05])

HSP 1 Score: 926.391 bits (2393), Expect = 0.000e+0
Identity = 451/858 (52.56%), Postives = 619/858 (72.14%), Query Frame = 0

Query:  130 DKVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRF--ASADLKPTGRTSRGCSAIKLRKTDEMVDITVLRPD--AAVDVDGPGDC-----------------GSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGS-GDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADSDDDDE 965
            ++++PT+L +E+S S+++YAMS I+GRALPDARDGLKPVHRRIL+AMH L L  +  +RKCARVVGEVLGKYHPHGD +VYDALVRMAQDF M  PL+AGHGNFGSVD+DP AAMRYTEC+L  L  DALL+DI  +TVDF  NFD ++ EP VLPAR+P LLLNG++GIAVGMATN+PPHNLGE++DG +ALIE+P ++D +L++ IP PDFPTG  I+G    ++ YT G GSI MR    +ETI    GR  R+AI++TELPYQ NKA+L+EKIA+MVNEK++EGI+D+RDESDR+G+RVVIELKRDA P VV NNL+K+T LQ++F  N LAL N   +PQ LTL+E LQ F+DFR + I RRT++EL K E RDH+++GL+ AL  +D+++ L+R A D   AK  L+ + Y +SEAQA AIL + L RLTALE  K+R+E E+L+  I + Q ++ + E++ ++++ E+ +++ KHA PRR+ I    G + E DL+AN+++++++TE GYIKRMP+  FE Q R TRGKA A++ +DD V HF  C DHD ++F SE+GV + ++A+Q+PV SR S+G P+ Q+LP+  ++ ++S++P+ E F +DE+LV+LTK G IKKT + AF  + + GLIAISL EGD L W RR   +D ++I +R+G ++ F      L+P GR +RG  ++KLRK DE+V + +L     A +D D   +                  G+GS G + L +T  GYGKRV    F++Q R G G+ A KFK     D LA +   + D E+++ T +G I+RQ V AI++QSR+ATGVRVQRLDE ++I  VA++P D+ D  E
Sbjct:    6 ERIIPTDLRIEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMHELGLSADRPFRKCARVVGEVLGKYHPHGDTAVYDALVRMAQDFSMRFPLIAGHGNFGSVDNDPPAAMRYTECRLEALTSDALLQDIESETVDFIDNFDASQQEPTVLPARIPQLLLNGSSGIAVGMATNIPPHNLGELIDGLVALIENPEITDIQLMQYIPGPDFPTGSQILGTTAIKEAYTTGRGSITMRGVATIETIEQ-RGRPDRDAIIITELPYQTNKAALIEKIAEMVNEKRLEGIADIRDESDRDGMRVVIELKRDAYPRVVLNNLYKQTPLQANFGANMLALVNG--EPQILTLKEFLQVFLDFRIEAITRRTRYELRKAEERDHILQGLLIALLHLDEIISLIRNASDAPTAKGELI-TTYGLSEAQADAILQMQLRRLTALEADKIRSEHEELQAIIANLQDILARRERILEIIQNEVTQLKQKHATPRRTVISPLEGEIDERDLIANEKALILLTEQGYIKRMPVSTFEAQSRATRGKAAAKIKEDDNVEHFLTCCDHDSILFFSERGVVYCLKAYQIPVGSRTSRGTPIVQMLPIPKEEKITSIVPVDE-FSSDEYLVMLTKGGNIKKTELAAFSNIRANGLIAISLEEGDQLRWVRRARVEDSVIIGSRQGMAIHFRCTHEQLRPLGRATRGVKSMKLRKGDELVGMDILPAAILATLDTDTEAEIEEIETEEIENEESTEVPGNGSTGPWALVITMGGYGKRVPVAQFRLQNRAGQGLTATKFKNRKIKDQLATLHIVNSDDEIMMVTSRGIIIRQAVNAISVQSRSATGVRVQRLDEDDVITGVAIVPPDTGDAAE 858          

BLAST of EWM24288.1 vs. NCBI_GenBank
Match: gi|1102818550|ref|WP_071599788.1| (DNA topoisomerase (ATP-hydrolyzing) subunit A [Mastigocladopsis repens])

HSP 1 Score: 926.006 bits (2392), Expect = 0.000e+0
Identity = 450/859 (52.39%), Postives = 618/859 (71.94%), Query Frame = 0

Query:  130 DKVLPTELNLELSSSFMQYAMSTILGRALPDARDGLKPVHRRILFAMHVLNLQPESSYRKCARVVGEVLGKYHPHGDASVYDALVRMAQDFVMSAPLVAGHGNFGSVDDDPAAAMRYTECKLSLLARDALLKDINLDTVDFAPNFDGNEVEPLVLPARVPMLLLNGATGIAVGMATNVPPHNLGEVLDGTLALIEDPSLSDDKLLRLIPAPDFPTGGDIMGLEGPRKLYTNGSGSIVMRAKTHMETIPMGGGRQVRNAIVVTELPYQVNKASLLEKIADMVNEKKMEGISDLRDESDREGIRVVIELKRDAMPSVVENNLFKKTALQSSFPGNFLALANDGRQPQRLTLRESLQSFIDFRFKTIRRRTQHELSKLESRDHVVEGLIRALDRIDDVVELVRRAKDTAAAKATLMGSQYDMSEAQAQAILALTLGRLTALEDAKLRAEREQLRGQIEDFQAMMRQDEKVFDVMKQELREIRAKHAVPRRSSIRAHAGALTELDLLANDRSVMMVTEAGYIKRMPLDDFEQQRRGTRGKAGARMSQDDLVNHFFACNDHDHVVFISEKGVAFGIRAFQVPVASRVSKGVPLPQVLPLSSDDHVSSVIPLGESFQADEFLVLLTKQGFIKKTPVDAFKKLTSRGLIAISLGEGDTLGWARRCTDQDDILISTRKGYSMRF--ASADLKPTGRTSRGCSAIKLRKTDEMVDITVLRPDAAVDVDGPGD--------------------CGSGSNGQYLLAVTTAGYGKRVSADSFKVQRRGGMGVIAIKFKAGS-GDSLACMRACSDDYEVVLSTQKGTIVRQRVAAIALQSRTATGVRVQRLDEGNIIKSVAMLPADSDDDDE 965
            ++++PT+L  E+S S+++YAMS I+GRALPDARDGLKPVHRRIL+AMH L L  E  ++KCARVVGEVLGKYHPHGD +VYDALVRMAQDF M +PLV GHGNFGSVD+DP AAMRYTEC+L  L  DALL+DI  +TVDF  NFD ++ EP VLPARVP LLLNG++GIAVGMATN+PPHNLGE++DG +ALIE+P ++D +L++ IP PDFPTGG I+G    R+ YT G GSI MR    +ETI    GR  R+AI++TELPYQ NKA+L+EKIA+MVNEK++EGI+D+RDESDR+G+R+VIELKRDA P VV NNL+K+T LQ++F  N LAL N   +PQ LTL+E LQ F+DFR + I RRT++EL K E RDH+++GL+ AL  +D+++ L+R A D   AK  LM + Y +SEAQA AIL + L RLTALE  K+R+E E+L+  I + Q ++ + E++ ++++ E+++++ KHA PRR+ I    G + E DL+AN+++++++TE GYIKRM ++ FE Q R TRGKA A+M +DD V HF  C DHD V+F SE+GV + ++A+Q+PV+SR S+G P+ Q+LP+  ++ ++S++P+ E F +DE+LV+LTK G IKKT + AF  + + GLIAISL EGD L W RR   +D ++I +R+G ++ F      L+P GR +RG  ++KLRK D +V + +L       ++                         G+GS G + L +T  GYGKRV    F++Q R G G++A KFK     D LA +   + D E+++ T +G I+RQ   AI++QSR+ATGVRVQRLDE ++I  VA++P D+ D +E
Sbjct:    6 ERIIPTDLRNEMSRSYLEYAMSVIVGRALPDARDGLKPVHRRILYAMHELGLIHERPFKKCARVVGEVLGKYHPHGDTAVYDALVRMAQDFSMRSPLVEGHGNFGSVDNDPPAAMRYTECRLQALTGDALLQDIESETVDFVDNFDASQQEPTVLPARVPQLLLNGSSGIAVGMATNIPPHNLGELIDGLVALIENPEITDIQLIQYIPGPDFPTGGQILGTSAIREAYTTGRGSITMRGVATIETIEQ-RGRPDRDAIIITELPYQTNKAALIEKIAEMVNEKRIEGIADIRDESDRDGMRIVIELKRDAYPRVVLNNLYKQTPLQANFGANMLALVNG--EPQILTLKEFLQVFLDFRIEAITRRTRYELRKAEERDHILQGLLIALAHLDEIINLIRHAPDAPTAKGELM-TTYGLSEAQADAILQMQLRRLTALEADKIRSEHEELQAIIANLQDILARRERILEIIQTEVKQLKEKHATPRRTVISPLEGEIDERDLIANEKALILLTEQGYIKRMAVNTFEAQSRATRGKAAAKMKEDDGVEHFLTCCDHDSVLFFSERGVVYCLKAYQIPVSSRTSRGTPIVQMLPIPKEEKITSIVPVTE-FTSDEYLVMLTKGGNIKKTELAAFSNIRANGLIAISLEEGDQLRWVRRARVEDSVIIGSRQGMAIHFRCTHEQLRPLGRATRGVKSMKLRKGDVLVGMDILPAAILATLEDTSTEAEIEEIETEEIENEESIEVSGNGSTGPWALVITMGGYGKRVPVSQFRLQNRAGQGLMATKFKNRKIKDQLATLHIVNSDDEIMMVTSRGIIIRQATNAISIQSRSATGVRVQRLDEDDVITGVAIVPPDTGDAEE 859