EWM30042.1, cds636 (CDS) Nannochloropsis gaditana

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|585112670|gb|EWM30042.1| (insulin-degrading enzyme [Nannochloropsis gaditana])

HSP 1 Score: 1912.12 bits (4952), Expect = 0.000e+0
Identity = 922/922 (100.00%), Postives = 922/922 (100.00%), Query Frame = 0

Query:    1 MEDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGVRTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYVHSSKHASDTCRPDVSETGRGVILVESMEALRKLKRGLSLFPAPGEVEWEGRE 922
            MEDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGVRTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYVHSSKHASDTCRPDVSETGRGVILVESMEALRKLKRGLSLFPAPGEVEWEGRE
Sbjct:    1 MEDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGVRTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYVHSSKHASDTCRPDVSETGRGVILVESMEALRKLKRGLSLFPAPGEVEWEGRE 922          

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|553189586|ref|XP_005855032.1| (insulysin [Nannochloropsis gaditana CCMP526] >gi|422294030|gb|EKU21330.1| insulysin [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1508.04 bits (3903), Expect = 0.000e+0
Identity = 729/733 (99.45%), Postives = 732/733 (99.86%), Query Frame = 0

Query:  189 QSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYVHSSKHASDTCRPDVSETGRGVILVESMEALRKLKRGLSLFPAPGEVEWEGR 921
            QSSKPARYLAHLLGHEGKGSLHSYLNSQGWIES+SAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLL+KVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHIS ATAPARRKLAVYVHSSKHASDTCRPDVSETG+GVILVESMEALRKLKRGLSLFPAPGEVEWEGR
Sbjct:  488 QSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESLSAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLEKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISVATAPARRKLAVYVHSSKHASDTCRPDVSETGKGVILVESMEALRKLKRGLSLFPAPGEVEWEGR 1220          

HSP 2 Score: 247.284 bits (630), Expect = 2.119e-64
Identity = 121/155 (78.06%), Postives = 124/155 (80.00%), Query Frame = 0

Query:    1 MEDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPP-----------------------------EKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQ 126
            MEDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEH+NNLQDDGWRLFQLQKSTAHPEHPFHKFGSGN +TLLPP                              +KGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQ
Sbjct:  218 MEDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHTNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNLRTLLPPQGNVPPCPSSSSLPPLPSPAQEEAGLTHTYQKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQ 372          

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|585112671|gb|EWM30043.1| (insulin-degrading enzyme [Nannochloropsis gaditana])

HSP 1 Score: 1358.58 bits (3515), Expect = 0.000e+0
Identity = 653/653 (100.00%), Postives = 653/653 (100.00%), Query Frame = 0

Query:    1 MEDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGVRTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKD 653
            MEDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGVRTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKD
Sbjct:    1 MEDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGVRTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKD 653          

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|299472245|emb|CBN77215.1| (similar to insulin-degrading enzyme [Ectocarpus siliculosus])

HSP 1 Score: 558.525 bits (1438), Expect = 1.090e-178
Identity = 351/987 (35.56%), Postives = 519/987 (52.58%), Query Frame = 0

Query:    2 EDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGVRTT---GQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMM--HEYPPENILWGPY-ALDWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEA-----WAKEKWYGTLHKLEKLPEAKVAAWSN-PPEIHPM---LSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQAL-PASLPASSPERGVK---------------------------VFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKG-------------------------------ANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKDSWGFS-PLYDGEQPEERAVMLHANSTLIYQTPG--------PNPEEDNSATEVYIQCGPTHLSGGDTKSDV--ILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQ-------------------------GLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYVHSSKHASD 878
            E TNYYF +K   L+GA DRF+ FF  P F  ++ EREM+AVDSEHSNN  +D WR++Q+ K+TA+P H F KFGSGN +TL P PE+G    TR +L+ FH TYYSAD MKL+I+G EDLDTL+ W    F+GVR T     P + PYP  A+  + LG R+ V+P++E R+L+L+WPLP      RAL  +       +  G+EG+G LH  L+ +GW+ S+SAGS V   D  +F++SLSLT+ G  H +EI+ L ++++ LLRS P  +++ ++L  M+EI F F E          I++ +   +  P  +L G +  L+WD  A+ D ++ LTP+   + VVSK  EEEA K+      W KE+WYGT +K+E L E  +      PP +        LP  N F+PT+FSL  +     + A  +       +     + + L PAS+PA + E   K                           ++HK+D +++VPK    A L + + Y+SP + +  R++  LL+++L  +AY+A +AGL YS+   T GL + V GYS  + +LL K+ G    LL E ++ G                               A    ++Q++   R + LR Y N  ++ PYE A Y  RQV++ + WH+ +Y Q L D   C    M    ++ + R+  D  AHGNV  +EA      + D+   + PL + E P   A+ L A                   +  E NSA +VY+Q GP     G++  D+   L++++ +  TSA+Q+LRT +QLGY+V+  L R   G+                           SVVVQSP  +P +L+  +E W+A FR+ EL  LSDE F+S + ++ S  L +++ + EEA   +  I  R  DFYR  R+    R LTK+ +L  +D    A  APARRKL+V V S +HA D
Sbjct:  169 ESTNYYFDVKSSHLRGATDRFAQFFRTPLFAESAIEREMQAVDSEHSNNKNEDTWRIYQVLKATANPSHAFSKFGSGNYETLRPRPEEG--VDTRASLIDFHETYYSADAMKLSILGNEDLDTLEAWVRDAFSGVRNTKPPAVPDYGPYP--AFGAAELGRRVTVIPLKETRQLALSWPLPPYQGVTRALLRN------LYSQGYEGEGGLHKLLHGRGWVSSLSAGSMVTGTDFQLFRLSLSLTEEGERHTDEIIELCHRFIALLRSEPPQKRIRDDLAAMTEIGFRFLENGGPSRAVQSIATTLGQEDVVPAEVLSGAFTVLEWDPAALTDVVNRLTPKNCQILVVSKKDEEEADKDGSAAIGWRKERWYGTSYKVEALSEELLRRLEGVPPHVEGFPEAFRLPGANPFIPTEFSLRADDAGEPAAAAQEGTATAGDADADAEAVRRLLPASIPALALETVPKEDWSRLVPPSLVAEGVGGEGGRGGAVNLWHKMDRSYRVPKSSIAAKLWTPEPYASPMAAMQARMFVRLLKEDLKSWAYDADLAGLRYSLEMTTRGLQLSVGGYSSTVALLLSKILGHIGDLLAEYRELGELMESGRGGGGGGAGVGVGGHAEGQGLAGLTARQELLRQRYETSRESFLRYYRNSAQDQPYETADYYVRQVMEAEVWHIDEYRQALEDRDACTPADMARHFDKILGRMRVDVMAHGNVGRKEAEDLASAIADALSRTEPLPEAELPTRNALRLQAAGEGEGGGGNGVVVVELEADEAEKNSAVQVYLQAGP-----GESNLDLASALELINTLGYTSAFQQLRTREQLGYMVYTHLERGPSGKVTPPASWDGAGAAGGGEEMHPGGPLAWSVVVQSPDKTPAELEERVEAWIAGFRD-ELAALSDEVFQSTVASMSSSVLRRERSMREEASIFFGAIASRTGDFYRRYRKAESYRRLTKQAVLDAYD-QFYAPGAPARRKLSVRVASQRHARD 1138          

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|873232065|emb|CEM06609.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 544.658 bits (1402), Expect = 1.674e-173
Identity = 338/987 (34.25%), Postives = 527/987 (53.39%), Query Frame = 0

Query:    2 EDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGVRTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYAL-DWDEQAVRDYLS-LLTPERMHLTVVSKAFEEEAQK--EAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSA------------------QALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTG-LSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKD-----------------------------KG-ANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKDSWGFSPLYDGEQPEERAVMLHANS------TLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLR---TLTKEDLLSFWDTHISAATAPARRKLAVYVHSSKH--------------ASDTCRPDVSETGRGVILVESMEALRKLKRGLSLFPA 912
            E TNYYF + ++ L GALDRFS FF  P F+  +T+RE+ AVDSE   N+Q D  RL+ L K+ AHP+HPF KF +GN +TL   P KG     R AL  FH  YYS + MKL +VGR+D++ L++W  ++F+ +    +P+ F YPVE +    LG +  VVP+R+++R+  ++P PH  +     + +KP  YL+++LG+EG GSL   L  +GW E ++A    +  D ++F++S+ LT  G++H+EEI  L +QYL L+ ++P  + +F+++  +  +KF F EK +   FA+ +S  + ++ P  +L GP  L  W  + + + L   L P     T++SK FE+ AQ+  + W KEKWYGTLH+   +   K+  W++P  IH  L+LP PN+ +P DFSL C+Q +  + A        P   +   SA                  Q    S P  S ER V++ HK+D  F+VPK   +  L       +P S +L+ ++  LL +ELN   Y+A+ AG+   +   + G +++ V G+S KLP+L+ +V    + LL E K+                             KG A   ++ ++F   R    R Y N      Y  A  +  +VL+ + +   Q+I  L++   CN + M   V+E +AR D   +  GN + EEA    +   D     PL +GE+P  R V L   S       ++ +    +  + NSA EV+ Q G   LS    + D++LD+L  +A  SA+ RLRTE QLGYIV   +R L G +G SV VQS  A P  L+  IE W+   R+ E+  +SD++F + +  + + K ++DKRL+EE+ R W +I  R++ F R++RE AVL+    ++K+ LL+ +D HI A TAP+RR LA +  S  H              A D    + +  G G +++ + + +R+ +R   L PA
Sbjct:  200 ETTNYYFKVAYDHLHGALDRFSQFFISPLFNEDATDREVNAVDSEFYKNVQSDQRRLWMLTKTMAHPKHPFSKFATGNLQTLSNEPHKG--RDVRAALKQFHEAYYSGNAMKLCVVGRDDVEQLEKWVQEMFSPIVNNDRPTVFEYPVEPFSYQSLGKKYQVVPIRDIKRIVFSFPYPHFED----FKETKPDMYLSYILGYEGAGSLRKLLVEKGWAEELNAAPYFEATDFNIFRISIDLTHEGLQHLEEITSLTFQYLALISNAPPSQALFDQIAKLRSMKFRFSEKIDPASFATLVSRNLFDFEPPRVLSGPALLTKWAPEDIHELLDHYLIPRNCIFTIISKEFEKHAQERPDGWKKEKWYGTLHREVNIDPKKLRDWAHPSSIHSKLTLPPPNDLVPVDFSLRCDQESPPADAERAATDGGPGEAMVQVSARRLDEDRGGGGGGEDDFPQRFRVSTPGESGERTVEILHKMDRKFRVPKGHLYLQLTVPSSQQNPASYLLNDIFMQLLGEELNPLLYDASQAGMGCQMDFYSRGDITISVGGFSEKLPLLMTRVLQAMQSLLTEYKNVHLPPPESSREEEDDDRWEDDDDIQEGLTKGQARKQKLLERFETVRQKEKRRYENSRFAESYHLASRSVNEVLNPRGYSWDQHISFLSNPRQCNPEEMARNVQESLARGDITCFVSGNFHREEAEDTARQAADVLRIDPLLEGEEPVNRCVELPLASGTPGGRGVVVEKTSVDANQPNSAVEVHFQVGLEDLS---FERDLLLDLLDQIAYRSAFNRLRTELQLGYIVDTMVRSLYGTRGFSVRVQSTVADPLALEQHIEGWLVKLRQ-EVVDMSDQEFLNSVNGLLAKKTQRDKRLAEESSRMWREISLRQFVFDRKEREAAVLQDNTKISKKRLLALFDEHI-AKTAPSRRLLASHFWSIPHRESHDKTAEAHAQQAGDKKAGEAAAAGDGGVVLRTHDDIRQFRRTRPLHPA 1175          

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|1129189175|gb|OLP94703.1| (Insulin-degrading enzyme [Symbiodinium microadriaticum])

HSP 1 Score: 537.339 bits (1383), Expect = 4.947e-169
Identity = 330/901 (36.63%), Postives = 491/901 (54.50%), Query Frame = 0

Query:    2 EDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGVRTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAV-DFASGISSMMHEYPPENILWGPYALD-WDEQAVRDYLSLLTPERMHLTVVSKAFEEE-----AQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIH-----PMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKD-----KGAND------------PEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALSYFQTLKDS---WGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYV 870
            E T YYF +    L  ALDRF+ FF  P FD +  EREMKAVDSE +N   +DGWRL Q+ K+TA PEHPF  F  GN  TL        ++ TR+ L+ ++ T+Y A  MKL +VG + LDTLQE A + F  V   G      YPV+ W  S++G  +  VP+++ R +S  WPLP     Q+ L  +KP  YLAHLLGHEG GSLH  LN  GW++ +S+G++    D  +F ++++LT  G  H EE++ L+++Y++ +RS+    ++F+EL ++ EI F  +E      DFA+  +  +H YP   +L GP+ALD W    V +YLS LTP    + V S  F EE     A  + W  E+WY   +KLE L +  +  W    E+        L LP PN F+P DFSL         +    P+   PP+ L  S                 ++++HK+D  FK P+    AH+ +    + P  V + RL+  ++ D+LN F+Y+A++AGL Y++      L++ V G++ KLP LLK V G+   LL+EI+      KGA+              E+ +K    R  LL++Y NF RE P+    Y   Q++  ++WHL++YI+VL      ++ A    V    + +  D   HGN  AEEA S   T+  S    G  PL +  + E   + L++ +   Y     NP ++N +T+V  Q GPT+    D   D  + ++  +A  SA+QRLRTE QLGYIV AF        G SV+VQ P   P ++D  IE W+ DF + EL  +++EDF+ ++ A+ + + ++  RL +EA RHWA+I+ RRY F R       L+++ KED+ +F++ ++ A  AP+RRKL+V V
Sbjct:  200 EYTTYYFKVASPFLDEALDRFAQFFISPTFDPSGIEREMKAVDSESTNYSTEDGWRLLQVLKATAAPEHPFFSFDVGNLDTL----GSNDLDNTRKQLVEWNKTHYQAGAMKLVVVGSDSLDTLQELATEKFCSV-PAGSGREMVYPVKPWPSSQVGRVVKCVPLKDSRSISACWPLP---PVQKYL-FAKPELYLAHLLGHEGAGSLHDALNQFGWVDQLSSGTAHSFTDEQLFAINITLTPEGDAHREEVLALLFEYVETIRSAGPKEEIFKELASLQEIGFAHKEDSPLPDDFAASAAMALHRYPANEVLRGPFALDEWRSDVVAEYLSTLTPTNCLIMVTSDGFREESTAQSASDQGWKPEEWYKAYYKLEALDKDHLDQWREKLEVALQGQPSGLKLPEPNRFIPNDFSLRASDGEGAEVT-KLPMEVLPPNPLMDSGM---------------LRLWHKVDKAFKTPREYVLAHVHTAAYDAGPEVVTMMRLFCSVVSDDLNTFSYDASVAGLGYNLEF-ADNLTMSVGGFNDKLPDLLKVVVGRIATLLEEIETIAEAVKGASSEEELLAALGDRGQELLEKLEVQRQILLQDYKNFTREDPWSVGNYYLSQLMLRKSWHLSEYIEVLERGP--DLAATAAAVRTAFSHVQVDMLVHGNATAEEAKSVADTVCASLKRLGAEPLKELRKKEITQLPLNSTTVFEYDLAALNPAQENCSTQVVYQVGPTN---EDLSRDACVSLVCRIAHVSAFQRLRTELQLGYIVQAFPWVNEHICGFSVLVQGPHEHPQKVDELIETWLEDFGQ-ELEAMTEEDFQKNVEALVNERTQRYSRLLQEATRHWAEILPRRYQFERILESTEALKSVKKEDVAAFFEEYL-AKGAPSRRKLSVRV 1067          

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|1126162426|ref|XP_019635766.1| (PREDICTED: insulin-degrading enzyme-like isoform X3 [Branchiostoma belcheri])

HSP 1 Score: 521.546 bits (1342), Expect = 1.952e-167
Identity = 310/882 (35.15%), Postives = 485/882 (54.99%), Query Frame = 0

Query:    2 EDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGV--RTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYAL-DWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALS----YFQTLKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYVHSSKHA 876
            E TNYYF +  + L+GALDRF+ FF  P F+  S +REM AVDSE+  NL+ D WRL QL+K+TA  +HPF KFG+GN+ TL   P +  ++  RE LL +H+TYYSA++M L+++G+EDLD+L    V  FA V  +    P    +P+  +    L  R DVVPV+++R +++A+PLP      R    + P  YL HL+GHEG GSL S L ++GW+ S+  G          F V++ LT+ G+ H+ +IV  ++QYL++LR +     +F E + +S +KF F++KE   ++ S ++ ++ +YP +++L  PY   D++ Q + D LS LTPE++ + VV+KAFE     E    EKWY   +++E +    +  WS   + HP L LP PNEF+PT F +   +P                    P  A ++P  +   +     KV+ K D TF +PK       ++   Y+ P +  L  ++  L RD L E+AY A +AG+SY++   T G  + + GY  K  +LLKKV  K              DP   ++F   +    R   NF  E PY+ A+Y T  +L   AW   + +  L++ +  N+Q+    V++  + +  +   HGN+  +EAL        +L+++    PL   ++ + R V L       Y+    N     SA E Y QCG        T+ +++L++L  + +   + +LRT++QLGYIVF+ +RR N  QGL  ++QS    P  LD  +E ++    E  L  LS+E+F+ H+ A+   +L+K K+L+ E  RHW +I+ ++Y+F R+  EVA L+T+TKE+LL+F+  H S   AP RRKL +YV  ++ A
Sbjct:   49 EHTNYYFDVSSQHLEGALDRFAQFFISPLFNEESKDREMNAVDSENEKNLKSDMWRLHQLEKATADQKHPFSKFGTGNKYTLSERPSQLNLDV-REELLKYHSTYYSANVMALSVLGKEDLDSLSRLVVDKFASVENKNVNVPE---FPIHPFQEEHLRLRCDVVPVKDIRTMNVAFPLPDLHPYYR----TNPGHYLGHLIGHEGPGSLLSLLKAKGWVNSLVGGQKEGGNGFMFFTVNVDLTEEGLGHVYDIVAHIFQYLEMLRQTGHQEWIFNECRDLSALKFRFKDKEMPRNYTSHLAGLLQKYPMDHVLVAPYLCEDYEPQLIEDVLSKLTPEKVRVAVVAKAFEGATDSE----EKWYKAQYRIEPISPDLIQQWSQ-VQTHPDLKLPPPNEFIPTQFEI---KPR-------------------PDEAPSIPECIKDDA---MCKVWFKQDDTFLLPKACLSFEFINPVAYADPLNCNLAYMFVQLYRDALTEYAYSAELAGVSYTLHNTTYGFYLAIGGYDDKQSILLKKVLDKMTSF--------TIDP---KRFTIIKELYTRALENFKAEQPYQHAVYYTTLLLSEMAWTKEELVGSLDEVTVENLQSF---VKQLFSHMYMECLVHGNLTQQEALDTVGLVVGSLQENTQTRPLLASQRVKHREVQLPQGVAHKYER--ENTVHSTSALETYYQCGLQ-----STRDNMLLELLCQILNEPCFNQLRTQEQLGYIVFSGVRRANSVQGLRFILQS-DRQPAYLDERVEVFVQKM-ESHLQDLSEEEFQKHVAALSVRRLDKPKKLTSETARHWGEILAQQYNFDRDNIEVAFLKTITKEELLNFYKEHFSWG-APKRRKLTIYVKPTEVA 868          

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|919040609|ref|XP_013403642.1| (PREDICTED: insulin-degrading enzyme-like [Lingula anatina])

HSP 1 Score: 522.702 bits (1345), Expect = 1.061e-166
Identity = 317/879 (36.06%), Postives = 497/879 (56.54%), Query Frame = 0

Query:    2 EDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGV--RTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYAL-DWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVN---AEEALSYFQT-LKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYVHSS 873
            E TNY+F +  E L+GA+DRF+ FF CP F  ++TERE+ AV+SE+  N+ +D WR  QL+K+TA+P+HPF KFG+GN+ TL   P++ G+  TR+ LL FH+ +YSA+IM L+++G+E LD LQE  + LF+ V  +    P    +P   + P +L  +  VVPV+++R LS+ WP+P   E  +    S P  YL HL+GHEG GSL S L ++GW+ ++  G S        F VS+ LT+ G+EH+++I+ LV+QY+K+L+       +F+EL+ +S ++F F++KE    +A  ++S++HEYP E  L   Y L D+    +   L +LTPE++ + VV K FE  A++    KEKWYGT + +EK+P   +  W N  + H  L LP  N+F+ TDF+++   P  K                  SS Q +    P S      +++ K D TF +PK  +   L S   Y  P    L+ ++  L RD LNE+AY+A +AGL YS+     G+ + + GYS K P+LLKKV  K              DP   ++F   +    R   NF  E P++ A+Y T  V   Q W   + +  L D     V+ +T ++ + ++RL+ +   +GNV    AEE L   Q  LK++    PL   ++   R V L    + +Y++   N    +S+ EVY QC        +T S+V+L++   + S   +  LRT++QLGYIVF+ +RR +G QGL ++VQS   SP  ++   E ++    E  +  ++D+ F+ H+ A+   +LEK K++S +  + W++I  ++Y+F R++ E A LRTLTK+D++ F+   IS   AP R K++V+V S+
Sbjct:  134 EHTNYFFDVAPEHLKGAMDRFAQFFLCPLFTASATEREVNAVNSENDKNIPNDAWRFNQLEKATANPKHPFSKFGTGNKYTLETRPKELGIN-TRDELLKFHSKFYSANIMGLSVLGKESLDELQEMVLDLFSNVENKEVAIPE---WPESPYGPEQLQVKAFVVPVKDIRNLSILWPIPDLQEHYK----SNPGHYLGHLIGHEGPGSLLSELKAKGWVNTLVGGQSGGAKGFMFFHVSVDLTEEGIEHVDDIITLVFQYIKMLKREGPQEWIFKELQDLSHMQFRFKDKERPQGYACQVASVLHEYPLEEALSHGYHLTDYRPDLISLVLDMLTPEKIRVGVVGKQFEGIAEQ----KEKWYGTAYCVEKIPGELIQMWEN-VDYHENLKLPQKNQFIATDFNIV---PREKEAT---------------SSPQVI-VETPLS------RLWFKQDDTFLLPKAYYAFELNSPLAYMDPLHTNLNAMFCDLFRDALNEYAYDAELAGLGYSLLGTVYGIYLGIRGYSDKQPILLKKVMEKMTTF--------KPDP---KRFEIFKEAYKRSLENFRAEQPHQHAVYYTNLVTSEQMWTKEELLDALED---ITVERLTAMIPQFLSRLNIEGLVYGNVTRQRAEEVLDIIQNMLKENCDTKPLAPSQKKRWREVQLPDGCSYLYKS--TNDVHKSSSIEVYYQCNLQ-----ETHSNVLLELFCQIISEPCFNILRTQEQLGYIVFSGIRRNSGVQGLRIIVQS-DRSPEYVESRTEAFLKKM-ESYILEMTDDIFKKHVEALAKKRLEKPKKMSAQNGKLWSEINCKQYNFDRDEIEAAHLRTLTKDDVMQFYKELISPE-APKRHKVSVHVVST 950          

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|1126162422|ref|XP_019635746.1| (PREDICTED: insulin-degrading enzyme-like isoform X2 [Branchiostoma belcheri] >gi|1126162424|ref|XP_019635757.1| PREDICTED: insulin-degrading enzyme-like isoform X2 [Branchiostoma belcheri])

HSP 1 Score: 520.776 bits (1340), Expect = 2.097e-166
Identity = 310/882 (35.15%), Postives = 485/882 (54.99%), Query Frame = 0

Query:    2 EDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGV--RTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYAL-DWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALS----YFQTLKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYVHSSKHA 876
            E TNYYF +  + L+GALDRF+ FF  P F+  S +REM AVDSE+  NL+ D WRL QL+K+TA  +HPF KFG+GN+ TL   P +  ++  RE LL +H+TYYSA++M L+++G+EDLD+L    V  FA V  +    P    +P+  +    L  R DVVPV+++R +++A+PLP      R    + P  YL HL+GHEG GSL S L ++GW+ S+  G          F V++ LT+ G+ H+ +IV  ++QYL++LR +     +F E + +S +KF F++KE   ++ S ++ ++ +YP +++L  PY   D++ Q + D LS LTPE++ + VV+KAFE     E    EKWY   +++E +    +  WS   + HP L LP PNEF+PT F +   +P                    P  A ++P  +   +     KV+ K D TF +PK       ++   Y+ P +  L  ++  L RD L E+AY A +AG+SY++   T G  + + GY  K  +LLKKV  K              DP   ++F   +    R   NF  E PY+ A+Y T  +L   AW   + +  L++ +  N+Q+    V++  + +  +   HGN+  +EAL        +L+++    PL   ++ + R V L       Y+    N     SA E Y QCG        T+ +++L++L  + +   + +LRT++QLGYIVF+ +RR N  QGL  ++QS    P  LD  +E ++    E  L  LS+E+F+ H+ A+   +L+K K+L+ E  RHW +I+ ++Y+F R+  EVA L+T+TKE+LL+F+  H S   AP RRKL +YV  ++ A
Sbjct:  107 EHTNYYFDVSSQHLEGALDRFAQFFISPLFNEESKDREMNAVDSENEKNLKSDMWRLHQLEKATADQKHPFSKFGTGNKYTLSERPSQLNLDV-REELLKYHSTYYSANVMALSVLGKEDLDSLSRLVVDKFASVENKNVNVPE---FPIHPFQEEHLRLRCDVVPVKDIRTMNVAFPLPDLHPYYR----TNPGHYLGHLIGHEGPGSLLSLLKAKGWVNSLVGGQKEGGNGFMFFTVNVDLTEEGLGHVYDIVAHIFQYLEMLRQTGHQEWIFNECRDLSALKFRFKDKEMPRNYTSHLAGLLQKYPMDHVLVAPYLCEDYEPQLIEDVLSKLTPEKVRVAVVAKAFEGATDSE----EKWYKAQYRIEPISPDLIQQWSQ-VQTHPDLKLPPPNEFIPTQFEI---KPR-------------------PDEAPSIPECIKDDA---MCKVWFKQDDTFLLPKACLSFEFINPVAYADPLNCNLAYMFVQLYRDALTEYAYSAELAGVSYTLHNTTYGFYLAIGGYDDKQSILLKKVLDKMTSF--------TIDP---KRFTIIKELYTRALENFKAEQPYQHAVYYTTLLLSEMAWTKEELVGSLDEVTVENLQSF---VKQLFSHMYMECLVHGNLTQQEALDTVGLVVGSLQENTQTRPLLASQRVKHREVQLPQGVAHKYER--ENTVHSTSALETYYQCGLQ-----STRDNMLLELLCQILNEPCFNQLRTQEQLGYIVFSGVRRANSVQGLRFILQS-DRQPAYLDERVEVFVQKM-ESHLQDLSEEEFQKHVAALSVRRLDKPKKLTSETARHWGEILAQQYNFDRDNIEVAFLKTITKEELLNFYKEHFSWG-APKRRKLTIYVKPTEVA 926          

BLAST of EWM30042.1 vs. NCBI_GenBank
Match: gi|1126162420|ref|XP_019635736.1| (PREDICTED: insulin-degrading enzyme-like isoform X1 [Branchiostoma belcheri])

HSP 1 Score: 521.546 bits (1342), Expect = 3.409e-166
Identity = 310/882 (35.15%), Postives = 485/882 (54.99%), Query Frame = 0

Query:    2 EDTNYYFSIKWEELQGALDRFSAFFTCPRFDNASTEREMKAVDSEHSNNLQDDGWRLFQLQKSTAHPEHPFHKFGSGNRKTLLPPPEKGGMEATREALLAFHTTYYSADIMKLAIVGREDLDTLQEWAVQLFAGV--RTTGQPSHFPYPVEAWDPSRLGTRLDVVPVREMRRLSLAWPLPHNWEKQRALQSSKPARYLAHLLGHEGKGSLHSYLNSQGWIESISAGSSVKTADMDMFKVSLSLTQAGMEHIEEIVGLVYQYLKLLRSSPVDRKVFEELKTMSEIKFLFREKENAVDFASGISSMMHEYPPENILWGPYAL-DWDEQAVRDYLSLLTPERMHLTVVSKAFEEEAQKEAWAKEKWYGTLHKLEKLPEAKVAAWSNPPEIHPMLSLPSPNEFLPTDFSLICEQPAYKSLAPDDPVHPFPPSLLFPSSAQALPASLPASSPERGVKVFHKLDTTFKVPKVQFFAHLLSRQIYSSPPSVVLHRLYNMLLRDELNEFAYEAAMAGLSYSVTTRTTGLSVKVSGYSHKLPVLLKKVAGKAKGLLQEIKDKGANDPEIQQKFNKHRLTLLREYMNFDREAPYERALYNTRQVLDGQAWHLAQYIQVLNDHSTCNVQAMTDVVEEGMARLDCDAYAHGNVNAEEALS----YFQTLKDSWGFSPLYDGEQPEERAVMLHANSTLIYQTPGPNPEEDNSATEVYIQCGPTHLSGGDTKSDVILDVLSHMASTSAYQRLRTEQQLGYIVFAFLRRLNGGQGLSVVVQSPSASPPQLDGFIEDWMADFREKELGTLSDEDFESHLLAVESMKLEKDKRLSEEAYRHWAQIVERRYDFYREKREVAVLRTLTKEDLLSFWDTHISAATAPARRKLAVYVHSSKHA 876
            E TNYYF +  + L+GALDRF+ FF  P F+  S +REM AVDSE+  NL+ D WRL QL+K+TA  +HPF KFG+GN+ TL   P +  ++  RE LL +H+TYYSA++M L+++G+EDLD+L    V  FA V  +    P    +P+  +    L  R DVVPV+++R +++A+PLP      R    + P  YL HL+GHEG GSL S L ++GW+ S+  G          F V++ LT+ G+ H+ +IV  ++QYL++LR +     +F E + +S +KF F++KE   ++ S ++ ++ +YP +++L  PY   D++ Q + D LS LTPE++ + VV+KAFE     E    EKWY   +++E +    +  WS   + HP L LP PNEF+PT F +   +P                    P  A ++P  +     +   KV+ K D TF +PK       ++   Y+ P +  L  ++  L RD L E+AY A +AG+SY++   T G  + + GY  K  +LLKKV  K              DP   ++F   +    R   NF  E PY+ A+Y T  +L   AW   + +  L++ +  N+Q+    V++  + +  +   HGN+  +EAL        +L+++    PL   ++ + R V L       Y+    N     SA E Y QCG        T+ +++L++L  + +   + +LRT++QLGYIVF+ +RR N  QGL  ++QS    P  LD  +E ++    E  L  LS+E+F+ H+ A+   +L+K K+L+ E  RHW +I+ ++Y+F R+  EVA L+T+TKE+LL+F+  H S   AP RRKL +YV  ++ A
Sbjct:  149 EHTNYYFDVSSQHLEGALDRFAQFFISPLFNEESKDREMNAVDSENEKNLKSDMWRLHQLEKATADQKHPFSKFGTGNKYTLSERPSQLNLDV-REELLKYHSTYYSANVMALSVLGKEDLDSLSRLVVDKFASVENKNVNVPE---FPIHPFQEEHLRLRCDVVPVKDIRTMNVAFPLPDLHPYYR----TNPGHYLGHLIGHEGPGSLLSLLKAKGWVNSLVGGQKEGGNGFMFFTVNVDLTEEGLGHVYDIVAHIFQYLEMLRQTGHQEWIFNECRDLSALKFRFKDKEMPRNYTSHLAGLLQKYPMDHVLVAPYLCEDYEPQLIEDVLSKLTPEKVRVAVVAKAFEGATDSE----EKWYKAQYRIEPISPDLIQQWSQ-VQTHPDLKLPPPNEFIPTQFEI---KPR-------------------PDEAPSIPECI---KDDAMCKVWFKQDDTFLLPKACLSFEFINPVAYADPLNCNLAYMFVQLYRDALTEYAYSAELAGVSYTLHNTTYGFYLAIGGYDDKQSILLKKVLDKMTSF--------TIDP---KRFTIIKELYTRALENFKAEQPYQHAVYYTTLLLSEMAWTKEELVGSLDEVTVENLQSF---VKQLFSHMYMECLVHGNLTQQEALDTVGLVVGSLQENTQTRPLLASQRVKHREVQLPQGVAHKYER--ENTVHSTSALETYYQCGLQ-----STRDNMLLELLCQILNEPCFNQLRTQEQLGYIVFSGVRRANSVQGLRFILQS-DRQPAYLDERVEVFVQKM-ESHLQDLSEEEFQKHVAALSVRRLDKPKKLTSETARHWGEILAQQYNFDRDNIEVAFLKTITKEELLNFYKEHFSWG-APKRRKLTIYVKPTEVA 968