EWM24359.1, cds6316 (CDS) Nannochloropsis gaditana

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|585105755|gb|EWM24359.1| (biotin and thiamin synthesis associated [Nannochloropsis gaditana])

HSP 1 Score: 1267.68 bits (3279), Expect = 0.000e+0
Identity = 606/606 (100.00%), Postives = 606/606 (100.00%), Query Frame = 0

Query:    1 MMLWRASGRLCPRSLFTTPTAASRGGKLPSRHHQQRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQLMSDNAKRVVVAMACVIDIDEKISRHIVQKKGVIFGRDNQTQN 606
            MMLWRASGRLCPRSLFTTPTAASRGGKLPSRHHQQRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQLMSDNAKRVVVAMACVIDIDEKISRHIVQKKGVIFGRDNQTQN
Sbjct:    1 MMLWRASGRLCPRSLFTTPTAASRGGKLPSRHHQQRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQLMSDNAKRVVVAMACVIDIDEKISRHIVQKKGVIFGRDNQTQN 606          

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|553187049|ref|XP_005854541.1| (Fe-Fe hydrogenase maturation protein [Nannochloropsis gaditana CCMP526] >gi|422294523|gb|EKU21823.1| Fe-Fe hydrogenase maturation protein [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1195.26 bits (3091), Expect = 0.000e+0
Identity = 571/572 (99.83%), Postives = 571/572 (99.83%), Query Frame = 0

Query:    1 MMLWRASGRLCPRSLFTTPTAASRGGKLPSRHHQQRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQLMSDNAKRV 572
            MMLWRASGRLCPRSLFTTPTAASRGGKLPSRHHQQRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQLMSDNAKR 
Sbjct:    1 MMLWRASGRLCPRSLFTTPTAASRGGKLPSRHHQQRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQLMSDNAKRA 572          

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|1039092114|gb|ANM86880.1| (HydG [Stygiella incarcerata] >gi|1039092161|gb|ANM86741.1| HydG [Stygiella incarcerata])

HSP 1 Score: 622.468 bits (1604), Expect = 0.000e+0
Identity = 309/541 (57.12%), Postives = 383/541 (70.79%), Query Frame = 0

Query:   23 SRGGKLPSRHHQQRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQ 563
            SRG    S    + +  +WS+E E HHK+  P +II++  ++R LE TK++AKD   V+ IL  A + A L+ + PG         E+VQGL  E+AA LLNVDS N  +M  L+  AL +K++IYGNRIVLFAP+YLAN+C+N+C YC+FR  NK+  R  L+ DE+ +EV+ L+ +GH+RLLLLTGE PKY+FD+FL AV +V +V+TP PCG IRRINVEIPSLSVSD RRLKAT  IGTYTLFQE+YH +T++++HP GPK+D+ HR+LTMDRAQ GG+DDVGIGALFGL DYRFEVLG+L HA HLDR Y  GPHT+SIPRMQPA NAP AE  P PV D +FKKLVA++RCAVPYTGMILSTRES ++RR LL LG+SQ+SAGSRT VG+YH+              G S        P   Q             EEE  E+      QF LQDHR LD VI DLL   F+PSFCTACYR+GRTG  FM+IAK G I +FC PNALL+LQEY++DYASP+T+ +G  VI  E +
Sbjct:   10 SRGAHATSTVLSRTFYHEWSVEREPHHKFAKPEEIINDAELHRALEVTKERAKDPIAVRDILQKAWDNALLKGVVPG--------EEYVQGLDNEDAAVLLNVDSTNDTLMGELFSTALKIKERIYGNRIVLFAPLYLANYCINSCTYCSFRHENKEGHRDCLSDDEIVEEVKELQQMGHRRLLLLTGESPKYTFDQFLNAVHIVASVRTP-PCGSIRRINVEIPSLSVSDFRRLKATNEIGTYTLFQESYHHETYKRVHPKGPKADFGHRLLTMDRAQTGGIDDVGIGALFGLYDYRFEVLGMLSHAKHLDRVYHAGPHTVSIPRMQPAANAPDAERPPYPVDDTNFKKLVAILRCAVPYTGMILSTRESPEMRRSLLHLGVSQMSAGSRTGVGSYHK--------------GESNTDLMNKIPKIEQA----------VSEEESGEQV----AQFTLQDHRTLDEVIHDLLKMKFVPSFCTACYRQGRTGEEFMRIAKKGDIHNFCHPNALLTLQEYLDDYASPETRNVGEHVISEEKE 513          

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|1183360558|gb|ORX87920.1| (radical SAM enzyme [Anaeromyces robustus])

HSP 1 Score: 618.231 bits (1593), Expect = 0.000e+0
Identity = 299/531 (56.31%), Postives = 382/531 (71.94%), Query Frame = 0

Query:   35 QRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQLM 565
            +R+ A WSIE E +HK++   +II E  I   LE TK+ AK+   ++ IL  A++RA L+ +  G        +E+VQGLT+EE ATLLN+DS NK++  LL+  A ++K++IYGNRIVLFAP+Y++N+CVN+C YC+FR  N    R++L  ++L +EV  L+  GHKRLL+LTGEHPKY F++FLEAV+++  V+TP PCGEIRRINVEIP LSVSD RRLK T  IGTYTLFQETYHR+TFR++HP GPKS+Y  R+LT DRAQ+GG+DDVG+G LFGL DY+FEVL LL HA HLD+TY  GPHT+SIPR+QPA NAP A   P PV+DDDF+KLVA++RCAVPYTGMILSTRES ++R +LL +GISQ+SAGSRTDVGAYHRDTS                     R A   L P    +++                QF L D R  D VIKDL+  G++PSFCTACYR+GRTG AFM IAK G I +FC PN+LL+LQEY+ DYAS +T+ +G E+I +E +L+
Sbjct:   30 KRFYA-WSIEREPNHKFKKANEIIREKEIYEALENTKEDAKNPEIIRNILKKAKDRALLKNIVAG--------SEYVQGLTIEETATLLNMDSKNKELTDLLFKTAFDIKERIYGNRIVLFAPLYISNYCVNSCTYCSFRIQNTHGTRALLEKEDLVKEVSVLQRQGHKRLLVLTGEHPKYPFEKFLEAVKIISEVRTP-PCGEIRRINVEIPMLSVSDFRRLKNTNVIGTYTLFQETYHRETFRRVHPHGPKSNYERRVLTFDRAQVGGIDDVGLGVLFGLYDYKFEVLALLQHAQHLDKTYKAGPHTLSIPRIQPADNAPDAMKTPYPVNDDDFRKLVAILRCAVPYTGMILSTRESTKMRNQLLNMGISQMSAGSRTDVGAYHRDTS---------------------RKADELLHPNTKYNST---------------SQFTLNDPRNTDQVIKDLIESGYVPSFCTACYREGRTGEAFMNIAKKGDIHNFCHPNSLLTLQEYLNDYASDKTRELGKELISKEEKLI 514          

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|1183397962|gb|ORY22735.1| (radical SAM enzyme [Neocallimastix californiae])

HSP 1 Score: 616.305 bits (1588), Expect = 0.000e+0
Identity = 300/532 (56.39%), Postives = 380/532 (71.43%), Query Frame = 0

Query:   35 QRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFI-SNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQLM 565
            +R+ A WSIE E +HK++  ++II E  I   LE+TK+ AK+   +K IL  A++RA L+ +  G        +E+VQGL +EE ATLLN+DS NK++  LL+  A ++K++IYGNRIVLFAP+YL+N+CVN+C YC+FR  N    R++L  +++ +EVE L+  GHKRLL+LTGEHPKY F++FL+A++V+  V+TP PCGEIRRINVEIP LSVSD RRLK T  IGTYTLFQETYHR+TF+++HP GPKS+Y  R+LT DRAQ  G+DDVG+G LFGL DY+FEVL LL HA HLD+TY  GPHT+SIPR+QPA NAP A + P PV+DDDF+KLVA++RCAVPYTGMILSTRESA +R +LL +GISQISAGSRTDVGAYHRDTS  A  F+  NT   S                                       QF L D R  D VI DL+  G++PSFCTACYR+GRTG AFM IAK G I +FC PN+LL+LQEY+ DYAS  TK IG ++I +E +L+
Sbjct:   31 KRFYA-WSIEREPNHKFKKASEIIKEKEIFEALEKTKEDAKNPEIIKNILKKAKDRALLKNIVAG--------SEYVQGLNIEETATLLNMDSTNKELTSLLFKTAFDIKERIYGNRIVLFAPLYLSNYCVNSCTYCSFRIQNTHGTRALLEKEDIVKEVEVLQRQGHKRLLVLTGEHPKYPFEKFLDALKVISEVRTP-PCGEIRRINVEIPMLSVSDFRRLKNTNVIGTYTLFQETYHRETFKRVHPHGPKSNYERRVLTFDRAQTAGIDDVGLGVLFGLYDYKFEVLALLQHAQHLDKTYHAGPHTLSIPRIQPAANAPDAINTPYPVNDDDFRKLVAILRCAVPYTGMILSTRESASMRNQLLNMGISQISAGSRTDVGAYHRDTSRKADEFMHQNTQYNSS-------------------------------------SQFTLNDPRNTDQVISDLIKSGYVPSFCTACYREGRTGEAFMNIAKKGDIHNFCHPNSLLTLQEYLTDYASENTKKIGEKLIEKEEKLI 515          

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|1198301343|gb|OUM58397.1| (hypothetical protein PIROE2DRAFT_16344, partial [Piromyces sp. E2])

HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0
Identity = 291/519 (56.07%), Postives = 370/519 (71.29%), Query Frame = 0

Query:   48 HHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFI-SNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQLM 565
            +HK++   +II E  I   LE+TK+ AK    +K+IL  A++RA L+ +  G        +E+VQGLT+EE ATLLN+DS NK++  LL+  A ++K++IYGNRIVLFAP+Y++N+CVN+C YC+FR  N    R++L  +E+ +EV  L+  GHKRLL+LTGEHPKY F++FL+A++V+  V+TP PCGEIRRINVEIP LSVSD RRLK T  IGTYTLFQETYHR+TF+K+HP GPKS+Y  R+LT DRAQ  G+DDVG+G LFGL DY+FEVL LL HA HLD+TY  GPHT+SIPR+QPA NAP A   P PV+DDDF+KLVA++RCAVPYTGMILSTRE+ ++R +LL +GISQ+SAGSRTDVGAYHRDTS  A  F+  NT   S +                                     QF L D R  D VI DL+  G++PSFCTACYR+GRTG AFM IAK G I +FC PN+LL+LQEY+ DYAS  TK +G E+I +E +L+
Sbjct:    1 NHKFKKANEIIREKEIYEALEKTKEDAKKPEIIKSILKKAKDRALLKNIVAG--------SEYVQGLTIEETATLLNMDSTNKELTNLLFKTAFDIKERIYGNRIVLFAPLYISNYCVNSCTYCSFRVQNTHGTRALLEKEEIEREVATLQRQGHKRLLVLTGEHPKYPFEKFLDALKVISEVRTP-PCGEIRRINVEIPMLSVSDFRRLKNTNVIGTYTLFQETYHRETFKKVHPQGPKSNYERRVLTFDRAQTAGIDDVGLGVLFGLYDYKFEVLALLQHAQHLDQTYNAGPHTLSIPRIQPAANAPDAMKAPYPVNDDDFRKLVAILRCAVPYTGMILSTRETTEMRNQLLNMGISQMSAGSRTDVGAYHRDTSRKADEFLHQNTEYNSSS-------------------------------------QFTLNDPRNTDQVIYDLIKSGYVPSFCTACYREGRTGEAFMNIAKKGDIHNFCHPNSLLTLQEYLHDYASENTKKVGEELIKKEEKLI 473          

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|1183326893|gb|ORX56653.1| (putative Fe-hydrogenase assembly protein [Piromyces finnis])

HSP 1 Score: 595.89 bits (1535), Expect = 0.000e+0
Identity = 291/524 (55.53%), Postives = 375/524 (71.56%), Query Frame = 0

Query:   41 WSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFI-SNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQ 563
            WSIE E +HK++  ++II E  I   LE+TK+ +K+   +K+IL  A+++A L+ +  G        +E+VQGLT+EE ATLLN+DS NK++  LL+  A ++K++IYGNRIVLFAP+Y++N+CVN+C YC+FR  N    R++L  +++ +EV  L+  GHKRLL+LTGEHPKY F++FL+A++V+  V+TP PCGEIRRINVEIP LSVSD RRLK T  IGTYTLFQETYHR+TF+++HP GPKS+Y  R+LT DRAQ  G+DDVG+G LFGL DY+FEVL LL HA HLD+TY  GPHTISIPR+QPA NAP A   P PV+DDDF+KLVA++RCAVPYTGMILSTRE+ ++R +LL +GISQ+SAGSRTDVGAYHRDTS  A   +  NT   S +                                     QF L D R  D VI DL+  G++PSFCTACYR+GRTG AFM IAK G I +FC PN+LL+LQEY+ DYAS +T+ IG E+I +E +
Sbjct:   36 WSIEREPNHKFKKASEIIREKEIFEALEKTKEDSKNPEVIKSILKKAKDKALLKNVVGG--------SEYVQGLTIEETATLLNMDSTNKELTSLLFKTAFDIKERIYGNRIVLFAPLYISNYCVNSCTYCSFRVQNTHGTRALLEKEDIEREVVTLQRQGHKRLLVLTGEHPKYPFEKFLDALKVISEVRTP-PCGEIRRINVEIPMLSVSDFRRLKNTNVIGTYTLFQETYHRETFKRVHPQGPKSNYERRVLTFDRAQTAGIDDVGLGVLFGLYDYKFEVLALLQHAQHLDQTYHAGPHTISIPRIQPAANAPDAIKTPFPVNDDDFRKLVAILRCAVPYTGMILSTRETTEMRNQLLNMGISQMSAGSRTDVGAYHRDTSKKADELLHQNTEYNSSS-------------------------------------QFTLNDPRNTDQVISDLIKSGYVPSFCTACYREGRTGEAFMNIAKKGDIHNFCHPNSLLTLQEYLTDYASEETRKIGEELIKKEEK 513          

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|552839247|ref|XP_005849755.1| (hypothetical protein CHLNCDRAFT_30311 [Chlorella variabilis] >gi|307109415|gb|EFN57653.1| hypothetical protein CHLNCDRAFT_30311 [Chlorella variabilis])

HSP 1 Score: 591.267 bits (1523), Expect = 0.000e+0
Identity = 302/559 (54.03%), Postives = 385/559 (68.87%), Query Frame = 0

Query:   19 PTAASRGGKLPSRH---HQQRWLAQWSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRESQL-MSDNAKRVV 573
            P  A+ G +L  R     QQR L QWS+E E    Y+    II + +I   LE+T+  AKD A V+ IL AA+ER+ L    PG        +E+VQGLT EE ATLLNVD GN+ IM  ++D A  +KQ+IYGNRIVLFAP+Y+AN+CVN C+YCAFR  NK ++RS LT  +LR+EV AL+  GH+RLL+LTGEHPKY+FD FL+A+  + +V+T  PCG IRRINVEIPSLS+SDMRRLKAT  IGTYTLFQETYHR TF+ MH  GPKSDY++R++T DRA   GLDDVG+G LFGL DY++EVL  LMHA+HL+R YG GPHTIS+PRM+PA  +  +   P  V D +FKKLVA++R AVPYTGMILSTRES ++R ELLR+G+SQ+SAGS+TDVGAYHRD S                                      K  E++  +     GQF L DHRP+  ++ DL+ +G++PS+CTACYRKGRTG  FMKIAK+G I +FC PN+L +LQEY+ DY S + K IG ++I RE  + +SD+A+ + 
Sbjct:   22 PAVAAVGARLLGRSGLLQQQRGL-QWSVEKEPQ-SYKAVDDIIQDHVIVAALEKTQQAAKDPARVRDILTAAKERSFLTNHKPG-------PSEYVQGLTYEECATLLNVDVGNEQIMSDIFDTAFAIKQRIYGNRIVLFAPLYIANYCVNNCRYCAFRQGNKSLERSALTDKQLREEVAALQQQGHRRLLVLTGEHPKYTFDSFLKAIDTISSVRT-EPCGNIRRINVEIPSLSLSDMRRLKATDKIGTYTLFQETYHRPTFKHMHIAGPKSDYDNRVMTHDRAMRAGLDDVGLGTLFGLYDYKYEVLATLMHANHLEREYGAGPHTISVPRMRPADGSDVSLAPPYAVDDANFKKLVAILRIAVPYTGMILSTRESPEMRTELLRVGMSQMSAGSKTDVGAYHRDDS--------------------------------------KATEDNLADLN---GQFTLADHRPVQDIVVDLMKEGYVPSWCTACYRKGRTGEHFMKIAKAGNIHNFCHPNSLFTLQEYLNDYGSEEAKQIGQDLIERERVVGLSDSAQNLT 529          

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|159466244|ref|XP_001691319.1| (hydrogenase assembly factor [Chlamydomonas reinhardtii] >gi|46403157|gb|AAS92602.1| Fe-hydrogenase assembly protein [Chlamydomonas reinhardtii] >gi|158279291|gb|EDP05052.1| hydrogenase assembly factor [Chlamydomonas reinhardtii])

HSP 1 Score: 588.186 bits (1515), Expect = 0.000e+0
Identity = 288/521 (55.28%), Postives = 366/521 (70.25%), Query Frame = 0

Query:   41 WSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRE 561
            WS+E E HH+Y +PA+ I+E  I++ LE +K  A+DA  V+ IL  A+E+A +    P +  +   ++EFVQGLTLEE ATL+NVDS N ++M  ++D AL +K++IYGNR+VLFAP+Y+AN C+NTC YCAFR ANK M+RS+LT D+LR+EV AL+  GH+R+L LTGEHPKY+FD FL AV V+ +VKT  P G IRRINVEIP LSVSDMRRLK T  +GT+ LFQETYHRDTF+ MHP GPKSD++ R+LT DRA   GLDDVGIGALFGL DYR+EV  +LMH+ HL+R Y  GPHTIS+PRM+PA  +  +   P PV+D DF KLVAV+R AVPYTGMILSTRES ++R  LL+ G+SQ+SAGSRTDVGAYH+D +      +S  A                                         GQF LQD RP + ++K L+ +G++PS+CTACYR+GRTG  FM I K+G I DFC PN+LL+LQEY+ DYA P  +  G +VI RE
Sbjct:   59 WSVERETHHRYRNPAEWINEAAIHKALETSKADAQDAGRVREILAKAKEKAFVTEHAPVNAES---KSEFVQGLTLEECATLINVDSNNVELMNEIFDTALAIKERIYGNRVVLFAPLYIANHCMNTCTYCAFRSANKGMERSILTDDDLREEVAALQRQGHRRILALTGEHPKYTFDNFLHAVNVIASVKT-EPEGSIRRINVEIPPLSVSDMRRLKNTDSVGTFVLFQETYHRDTFKVMHPSGPKSDFDFRVLTQDRAMRAGLDDVGIGALFGLYDYRYEVCAMLMHSEHLEREYNAGPHTISVPRMRPADGSELSIAPPYPVNDADFMKLVAVLRIAVPYTGMILSTRESPEMRSALLKCGMSQMSAGSRTDVGAYHKDHTLSTEANLSKLA-----------------------------------------GQFTLQDERPTNEIVKWLMEEGYVPSWCTACYRQGRTGEDFMNICKAGDIHDFCHPNSLLTLQEYLMDYADPDLRKKGEQVIARE 534          

BLAST of EWM24359.1 vs. NCBI_GenBank
Match: gi|302833844|ref|XP_002948485.1| (hydrogenase assembly factor [Volvox carteri f. nagariensis] >gi|300266172|gb|EFJ50360.1| hydrogenase assembly factor [Volvox carteri f. nagariensis])

HSP 1 Score: 584.719 bits (1506), Expect = 0.000e+0
Identity = 294/521 (56.43%), Postives = 366/521 (70.25%), Query Frame = 0

Query:   41 WSIETEHHHKYEDPAKIIDEGLINRLLEETKDKAKDAACVKAILVAAQERARLQAMPPGHPTADPPQNEFVQGLTLEEAATLLNVDSGNKDIMQLLYDAALNVKQQIYGNRIVLFAPVYLANWCVNTCQYCAFRGANKQMQRSMLTMDELRQEVEALEAIGHKRLLLLTGEHPKYSFDRFLEAVQVVGTVKTPNPCGEIRRINVEIPSLSVSDMRRLKATQYIGTYTLFQETYHRDTFRKMHPGGPKSDYNHRILTMDRAQLGGLDDVGIGALFGLADYRFEVLGLLMHAHHLDRTYGTGPHTISIPRMQPALNAPAAEHIPLPVHDDDFKKLVAVIRCAVPYTGMILSTRESAQIRRELLRLGISQISAGSRTDVGAYHRDTSCDAAPFISNTAGTSGNGYRAGRPAKSQLPPPATLDASRKEEEEDTEEYKLRRGQFCLQDHRPLDTVIKDLLHDGFIPSFCTACYRKGRTGAAFMKIAKSGRIQDFCQPNALLSLQEYMEDYASPQTKAIGAEVIHRE 561
            WS+E E HH Y DPA+ IDE  I + LE TK+ AKD A V+ IL  A+E+A +    P +  +   ++EFVQGL+LEE ATLLNVD  N ++M  ++D AL +K +IYGNR+VLFAP+Y+AN C+NTC YCAFR  N  M+RS+LT D+LR+EV AL+  GH+R+L LTGE PKYSF+ FL AV V+G+V T  P G IRRINVEIP LSVSDMRRLK T  +GT+ LFQETYHR+TF+KMHP GPKSD+++R+LT DRA   GLDDVGIGALFGL DYR+EV  +LMH+ HL+R Y  GPHTIS+PRM+PA  +  +   P PV D  FKKLVAVIR AVPYTGMILSTRES ++R  LL+ G+SQ+SAGSRTDVGAYH+D          +TA T  N    G+ A                            GQF L DHRP + ++  L+ +G++PS+CTACYR+GRTG  FMKI K+G I DFC PN+LL+LQEY++DYA P  +  G EVI RE
Sbjct:   50 WSVERETHHTYVDPAEWIDEAAIFKALELTKEHAKDPARVRDILAKAKEKAFVTEHDPVNVGS---KSEFVQGLSLEECATLLNVDENNVELMNDIFDTALAIKNRIYGNRVVLFAPLYIANHCMNTCTYCAFRAGNTGMERSVLTDDDLREEVAALQQQGHRRILALTGESPKYSFENFLHAVDVIGSVHT-EPFGSIRRINVEIPPLSVSDMRRLKETDKVGTFVLFQETYHRETFKKMHPSGPKSDFDYRVLTQDRAMRAGLDDVGIGALFGLFDYRYEVCAMLMHSEHLERHYNAGPHTISVPRMRPADGSELSISPPYPVDDATFKKLVAVIRIAVPYTGMILSTRESPEMRSLLLKCGMSQMSAGSRTDVGAYHKD----------HTANTEAN---LGKLA----------------------------GQFSLMDHRPTNEIVDWLMAEGYVPSWCTACYRQGRTGEDFMKICKAGDIHDFCHPNSLLTLQEYLQDYADPDLRRKGEEVIARE 525