EWM24536.1, cds6136 (CDS) Nannochloropsis gaditana

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|585106044|gb|EWM24536.1| (lipolytic protein g-d-s-l family [Nannochloropsis gaditana])

HSP 1 Score: 1117.84 bits (2890), Expect = 0.000e+0
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 0

Query:    1 MTMSNVRSTSKIVRSEANDAKKVKVTYSVMANVDLKPAWLSFMLFVFVMSNFVRDAWAITSRMGTVPSFQKQQDLPSFYTATDDDINWSGRVLRNEEYMRVVFDWPGVTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTLRGVFSYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTDGFLSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGWKWSDVGWGKGWWCLLRQRKVGDGAKTSCFMGDEGLNVSTSSCPVYSLRLPVPFGGFLQASK 542
            MTMSNVRSTSKIVRSEANDAKKVKVTYSVMANVDLKPAWLSFMLFVFVMSNFVRDAWAITSRMGTVPSFQKQQDLPSFYTATDDDINWSGRVLRNEEYMRVVFDWPGVTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTLRGVFSYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTDGFLSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGWKWSDVGWGKGWWCLLRQRKVGDGAKTSCFMGDEGLNVSTSSCPVYSLRLPVPFGGFLQASK
Sbjct:    1 MTMSNVRSTSKIVRSEANDAKKVKVTYSVMANVDLKPAWLSFMLFVFVMSNFVRDAWAITSRMGTVPSFQKQQDLPSFYTATDDDINWSGRVLRNEEYMRVVFDWPGVTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTLRGVFSYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTDGFLSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGWKWSDVGWGKGWWCLLRQRKVGDGAKTSCFMGDEGLNVSTSSCPVYSLRLPVPFGGFLQASK 542          

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|553188142|ref|XP_005854742.1| (endoglucanase e [Nannochloropsis gaditana CCMP526] >gi|422294320|gb|EKU21620.1| endoglucanase e [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 614.764 bits (1584), Expect = 0.000e+0
Identity = 298/299 (99.67%), Postives = 298/299 (99.67%), Query Frame = 0

Query:  184 MVHTQEAQFLSNNAAVNNVTVLGFGTDGFLSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGWK 482
            MVHTQEAQFLSNNAAVNNVTVLGFGTDGFLSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSS KGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGWK
Sbjct:    1 MVHTQEAQFLSNNAAVNNVTVLGFGTDGFLSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSPKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGWK 299          

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|831769621|ref|XP_012752761.1| (hypothetical protein SAMD00019534_076100 [Acytostelium subglobosum LB1] >gi|735853393|dbj|GAM24435.1| hypothetical protein SAMD00019534_076100 [Acytostelium subglobosum LB1])

HSP 1 Score: 120.553 bits (301), Expect = 2.296e-26
Identity = 110/411 (26.76%), Postives = 181/411 (44.04%), Query Frame = 0

Query:   86 INWSGRVLRNEEYMRVV-FDWPGVTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTLRGVF--------SYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTD--GFLSRPS-PSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEV-TMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDL--MLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGW 481
            I + GRV  N +      FDWPGV    +V   +T +  +VD + +  +   +F  + T  +  +    TL+ V         +Y L + G+L        P+ T  +  T+  +     A +  V   GF  D    L  P+ P +RRLEF+GDSIT GYG  G++PC  + ET N  LT+   + + L+A+  +EA+SG+G+  +Y  P    V   P+ F  TLA+            +P   W  D+  ++P+A++ NLGTNDF     + +   + +   Y Q +  +   Y                           P +        P +FL  GPM +   GA    V+ +     +   +DL  +L+P+ +  GC GHP  + H  ++  + P+++  MGW
Sbjct:   30 IQYKGRVAANADTNGAYNFDWPGVEIIIKVTGTST-IQGLVDSNVVQASGLPLFLGNITIFNVFVN--GTLQSVLNVTSETKQTYNLFEDGLL-------NPSETCEVRLTKRTE-----ACLGVVGFYGFIVDHTATLVPPTEPPSRRLEFLGDSITCGYGIIGMSPCPFTPETENNYLTYGQLIARELEADVNIEAWSGIGLVRNYGEPNISSVLAFPDVFPRTLAT------------NPSLLWDFDS--YQPNAVVINLGTNDF---DSQPNPPADLFEQTYLQLIKTLNTVY---------------------------PNQ--------PKIFLVCGPMIS---GACCDYVKSVAESSDQLTYIDLQNILEPSDI--GCQGHPNISGHIKMSNISLPIIQKVMGW 368          

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|330805349|ref|XP_003290646.1| (hypothetical protein DICPUDRAFT_37730 [Dictyostelium purpureum] >gi|325079212|gb|EGC32823.1| hypothetical protein DICPUDRAFT_37730 [Dictyostelium purpureum])

HSP 1 Score: 102.449 bits (254), Expect = 3.998e-20
Identity = 109/407 (26.78%), Postives = 175/407 (43.00%), Query Frame = 0

Query:   82 TDDDINWSGRV-LRNEEYMRVVFDWPG--VTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTLRGVFSYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTDGFLSRPS---PSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHP-GTVEVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGW 481
            ++ DI ++GR+   N +Y    F+W G  +T +F       ++ V++D +      F V  ++  A +       T R +++       I+G   L   P+ T ++V ++  +     A+   V   GF  D   S      P+ R++EFIGDSIT GYG  G +PC  + +T N  +T+   + + L AE ++EA+SG GV  +Y  P  T   TMP+ +   L +     + TT        W  D   F+PDA++ NLGTND+      +    E +   Y  FV+ +   Y                    P +               P  FL  GPM    Q   + V    +  G   I L+ +L    +  GC GHP    H+ +A  A P+++ T+GW
Sbjct:   23 SNSDIYYTGRMDTTNPDYY--TFEWSGCQITLTF---TGTKSIKVVLDSAG--DNWFDVIINN--ALYGAFNVSSTPRQMYN-------IIGQNNLN--PSQTYTLVLSKRTE-----ASFGPVRFYGFVVDSTCSTKQYSPPTNRKIEFIGDSITCGYGDLGTSPCPFTPDTENNYVTYGAIIARELQAELYLEAWSGKGVVRNYGSPTSTSTQTMPDLYPYILPT-----DTTT-------NWDFDL--FQPDAVVINLGTNDYSTQPAPSQ---EVFQNGYISFVNSLKSNY--------------------PNK---------------PEFFLVCGPMIG--QPCCQYVKNVSSIVGATYIDLEGILTSDDI--GCNGHPNEQGHSIMASIASPIIQKTLGW 350          

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|470500787|ref|XP_004347088.1| (GDSLlike lipase/acylhydrolase domain containing protein [Acanthamoeba castellanii str. Neff] >gi|440800671|gb|ELR21706.1| GDSLlike lipase/acylhydrolase domain containing protein [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 101.293 bits (251), Expect = 1.097e-19
Identity = 112/414 (27.05%), Postives = 167/414 (40.34%), Query Frame = 0

Query:   86 INWSGRVLRNEEYMRVV-FDWPGVTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTLRGVFSYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGF--GTDG---FLS------RPSP-STRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEVT----MPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPM-SARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGW 481
            + + GRV ++      V F+W  V    ++  +  A+  ++D +   G  F VF D  +     +    T  GV  YT+A        GL     VT S+V  +  + L      +   V G   G DG   F+       RP    +RR+EF+GDSI+ GYG +G  PC  +  T N  LT+   + +SL AE  +E +SG GV  +Y   G   +T     P  F  TL +AH               W     T  P A++ NLGTND+     + +   + +   Y +FV  +   Y                                 G    P  FL  GPM S       + VV++   +GV    +D+    +    GC GHP+ + H  +A  A PV++A MGW
Sbjct:   23 VQYVGRVQQSSTVSGAVEFEWSNV----EIHTSGKAIEALLDSN---GNVFEVFVDGVS-----VGLINTTSGVRQYTVAS-------GL--STGVTHSVVLRKRTEALFGVVTFHGFEVAGASSGDDGERFFIPAVKATMRPKALESRRIEFVGDSISCGYGIEGHPPCPFTAATENSGLTYGSLIAKSLSAELHLECWSGRGVVRNY---GDKNITSAEPFPSLFPRTLPTAHDSA------------WHFGVWT--PQAVVINLGTNDYST---QPAPPQDIFEEGYQKFVEYIYSQYK--------------------------------GNHETPHFFLACGPMISQPCCSYVKNVVDKEKAKGVNITYIDMQHILSPEDHGCAGHPSVSGHRKMAGVAGPVIKAAMGW 363          

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|1160569633|ref|XP_020438071.1| (esterase [Polysphondylium pallidum PN500] >gi|281211803|gb|EFA85965.1| esterase [Polysphondylium pallidum PN500])

HSP 1 Score: 100.523 bits (249), Expect = 2.291e-19
Identity = 103/417 (24.70%), Postives = 165/417 (39.57%), Query Frame = 0

Query:   80 TATDDDINWSGRV--LRNEEYMRVVFDWPGVTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTL--------RGVFSYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTD--GFLSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEVT-MPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDL--MLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGW 481
            + +++++ + GRV    N+ Y    FDW GV  S  +   AT + V++D             ++PT     +  F  L          + S  +    ++    L    +  I+++   EA       ++  +   GF  D    +  P    R+LEF GDSIT GYG D LAPC  + +T N   T+ + + + L  +     +SG G+  +Y  P       MP     TLA+   L+            W  D + + PDA++ NLGTND+           E     Y   +  +  +Y                    P +               P +FL  GPM +   GA    VE ++     A  +DL  +LD +    GC GHP  + HA +AE A+P+++  M W
Sbjct:   28 SPSNENLLYMGRVDSSSNDVYY---FDWSGVGISMIITGTAT-LKVLMDT------------NTPTPSTNNINMFTVLINNAVYPPLNITSTEMESYDLVSYGTLVPTESYIITLIKRTEA-------SLGVIGFYGFMVDQAAVIVLPPSFPRKLEFFGDSITCGYGIDALAPCPFTPQTENNFDTYEYIISRQLSTQLHTACWSGKGLVRNYDSPNITSTNPMPIYLPRTLANNASLE------------W--DFANYVPDAVVINLGTNDYSTQPSPPENLFE---QTYINLIQTINNWY--------------------PNK---------------PKIFLVCGPMIS---GAVCDYVESVSETVESATYIDLQNILDDSDY--GCNGHPNQSGHAKMAEIAYPIIQKVMQW 364          

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|831789587|ref|XP_012759107.1| (hypothetical protein SAMD00019534_002200 [Acytostelium subglobosum LB1] >gi|735859739|dbj|GAM17045.1| hypothetical protein SAMD00019534_002200 [Acytostelium subglobosum LB1])

HSP 1 Score: 99.7525 bits (247), Expect = 3.193e-19
Identity = 102/420 (24.29%), Postives = 163/420 (38.81%), Query Frame = 0

Query:   84 DDINWSGRV----LRNEEYMRVVFDWPGVTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTLRGVF---------SYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTDG---FLSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTV-EVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCG-RGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPM----SARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGW 481
            ++I + GR+    L NE      FDWPG+  +  + +  +++  ++     +     + Y         +    T   VF         SY+L D G L +     +    ++++   EA F         V+  GF  D     L  P P +RRL F+GDSIT GYG   + PC  SVET N    +   + + L+A+ +  A+SG+G+  +   P T  +V   + +  TLA+   L            +W  +A  + PDA+I NLGTNDF          F+E Y       V  +   Y                                   +  P +FL  GPM       Y  +    ++ +T   ++ IL+      T    GC GHP    +  +A  A P+++  M W
Sbjct:   19 ENIQYKGRIDTSNLNNEG--AYYFDWPGIQITLVI-KGTSSIKAVLQSKLALNDIGDLHYSKGNKNIFDIVVNGTFHSVFTISSYDPKMSYSLFDDGYLSS-----DQTYVVTLIKRTEAAF-------GRVSFFGFIVDSTYMLLPPPIPRSRRLLFLGDSITCGYGNMVIDPCEFSVETENVNFAYGSLIAKQLEADAYFIAWSGIGLSRNCDSPDTTSKVVFKDMWTRTLATNSSL------------QW--EAGLYLPDAVIINLGTNDFITQPNPPRDIFIEDYT----NLVKAIHNIY-----------------------------------SNKPKIFLACGPMIGDPCCSYVQSVADSIDGVTYIDMQNILV------TANDIGCRGHPGKTGNIKMANIACPIIQKVMEW 364          

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|470244984|ref|XP_004356224.1| (esterase [Dictyostelium fasciculatum] >gi|328869362|gb|EGG17740.1| esterase [Dictyostelium fasciculatum])

HSP 1 Score: 97.4413 bits (241), Expect = 1.675e-18
Identity = 104/403 (25.81%), Postives = 164/403 (40.69%), Query Frame = 0

Query:   83 DDDINWSGRVLRNEEYMRVVFDWPGVTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTLRGVFSYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTDGF--LSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTV-EVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRG-ENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLDLMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGW 481
            D ++ ++GRV + +  + V FDW  VT +F V    +        +++    F V        + +L +      + S  +  + +L     +   NV+IS             AA+  V+  G   D     +   PS+R++EFIGDSIT GYG  GL PC  + ET +  +T+   + ++L AE +VE++SG G+  +     T  +   P  +  T+A+     N +T        W  +   F+PDA++ NLGTND+        S F + Y A      SK                                           P  FL  GPM +      + V +  T+ G   I L  +L P     GC GHP    H  +A+ AFP+++ TM W
Sbjct:   20 DQNLYYTGRVDKQDSNVYV-FDWSFVTINFIVTGTLSIKASFSTSNTVNPNLFNV------VVNDQLLDIPIT--INSTNIQVITLLDDLDPQSSYNVSISK---------RTEAAMGIVSFYGLEVDSTAQFTPLQPSSRKIEFIGDSITCGYGILGLGPCSFTPETEDSYVTYEGLISRALGAELYVESWSGKGMVRNVGDKNTTSQYPFPYYYNFTVAN-----NAST-------IWTYEG--FQPDAVVINLGTNDYSTQPAPPQSIFTQGYLAFIQLLKSKYTN---------------------------------------NPTFFLACGPMISN--PCCQYVKDIATQTGSTFINLQGLLTPE--STGCAGHPNQLGHTYMAKVAFPIIQQTMDW 347          

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|753218749|ref|WP_041524034.1| (hypothetical protein [Gilvimarinus agarilyticus])

HSP 1 Score: 97.4413 bits (241), Expect = 2.081e-18
Identity = 110/386 (28.50%), Postives = 158/386 (40.93%), Query Frame = 0

Query:  100 RVVFDWPGVTASFQVDENATAVTVMVDDSSLVGTRFAVFYDSPTARHRKLREFRTLRGVFSYTLADVGILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTDG-FLSRPSPSTRRLEFIGDSITAGYGADGLAP-CVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASYPHPGTVEVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPM-SARYQGATRGVVERLTRRGVRAILLDL-MLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGW 481
            R  F WPG +  F  +  + A+ +    +S    RF V  D   +      E     G   YTL         GL  +P+    +  T+ A+  S   A+ +  V    TDG  L+ P+P++RRL  IGDSITAGYG +G +  C  S ET++ +  +A    Q L A+    A+SG+GV+ SY        T+ ER   TL                 F  + D + +RPDA+I  +GTNDF  G          Y AA  +FVS+V             D P RP                         ++L V PM S   + A R  +  +    VR  +LDL  ++P     GC  HP       + E    ++E  +GW
Sbjct:   50 RAAFTWPGSSVQFNFNGTSAAIRL----ASAADIRFMVEVDGEQS------ELWVKAGEADYTLT-------SGLEAKPH---QVRLTRLAESFSGVTALVSDPV----TDGQLLAPPAPASRRLLVIGDSITAGYGVEGASKACSYSQETSSPLRAYAGVAAQRLSADVHTIAWSGIGVWRSYGEETPQAPTIAERRLLTLGD--------------DFDNEWDTTRYRPDAIIIAIGTNDFWLGSAPG------YRAAMGEFVSQV-----------QTDYPERP-------------------------IYLMVSPMLSGDARAAQRADLAAIASGDVR--VLDLGKIEPDDGY-GCDYHPNLVTQQRLGEALVQILERELGW 352          

BLAST of EWM24536.1 vs. NCBI_GenBank
Match: gi|66806859|ref|XP_637152.1| (esterase, SGNH hydrolase-type [Dictyostelium discoideum AX4] >gi|60465548|gb|EAL63632.1| esterase, SGNH hydrolase-type [Dictyostelium discoideum AX4])

HSP 1 Score: 94.7449 bits (234), Expect = 3.192e-17
Identity = 87/321 (27.10%), Postives = 130/321 (40.50%), Query Frame = 0

Query:  168 ILGAQGLRFEPNVTISMVHTQEAQFLSNNAAVNNVTVLGFGTD--GFLSRPSPSTRRLEFIGDSITAGYGADGLAPCVGSVETNNYVLTWAHALCQSLDAECFVEAYSGVGVYASY--PHPGTVEVTMPERFADTLASAHQLKNMTTGGLSPGFRWKVDASTFRPDALIFNLGTNDFCCGRGENSTFVEAYAAAYYQFVSKVLEFYAVPAGTRAKDLPIRPSSRKGPARAGDEPAEAPLGAAPAPVVFLGVGPMSARYQGATRGVVERLTRRGVRAILLD---LMLDPTVLMGGCLGHPTGAVHAAVAEKAFPVVEATMGW 481
            I+G+  L  +P  T S+V ++  +     A+   V   GF  D  G        TR++EFIGDSIT GYG  G APC     T +   T+A    + L+ E F+EA+SG GV  +Y  P P +   T+P+ +  T+ +             P   W  D + + PDA++ NLGTND+      +    E +   Y +F+  +   Y                                  A   P +FL  GPM           V  L    V A  +D   ++ +PT    GC GHP+   H  +     P+++  M W
Sbjct:  165 IIGSTNL--DPTQTYSIVLSKRTE-----ASFGEVRFYGFSVDSTGKTVPQQKPTRKIEFIGDSITCGYGDLGTAPCNFQAITEDNYDTYASITARELECEMFLEAWSGRGVVRNYGSPTPTSTSGTVPDLYPYTIPT------------DPSIEW--DFNQYIPDAVVINLGTNDYSTQPVPSQ---EQFQNGYIEFIKTIKSNY----------------------------------AQAEPEIFLICGPMIGNPCCEYVANVSTL----VGATFIDAQNILTEPTDF--GCNGHPSINGHMKLGAVISPIIQKVMAW 421