EWM24585.1, cds6095 (CDS) Nannochloropsis gaditana

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|585106101|gb|EWM24585.1| (DNA topoisomerase, type IIA, conserved site [Nannochloropsis gaditana])

HSP 1 Score: 3162.47 bits (8198), Expect = 0.000e+0
Identity = 1538/1538 (100.00%), Postives = 1538/1538 (100.00%), Query Frame = 0

Query:    1 MENVPKAKKKGASAKASASTAATMKAPLGESSSSINAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREAGSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELFAPGAGGYDEEEGEGGGELGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKARALDSDGDSFSEESEVEEEDDSDYAPSPKKKKGKANANAKSAMIKAHVPEPRVIVPIRKAPSAPKPPAAPKLAVSTSNKTSQGPVPGTKKKSRPARSSEDEDEDVDEDVQVLSLAERMRRRMQVSPAPRAVAGDEDKGAPGLSSGSPVPPRGTGLPPRTAATNTVLYSEGGESEEEAVAADAYMIMSPPPKKAPAASKTKVAGGAGAVKRANKKTGGGKGGMEGEGHAKEALFSPLKGTSPAVVKKARKAAAPASKARGATKKTNKAKSGSEEEEEEEEEEEMTASPVAPRTMAPRRAAAKKVVYDMSEEEGEEEEEEEPDSEFEEEEEDDSDFEG 1538
            MENVPKAKKKGASAKASASTAATMKAPLGESSSSINAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREAGSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELFAPGAGGYDEEEGEGGGELGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKARALDSDGDSFSEESEVEEEDDSDYAPSPKKKKGKANANAKSAMIKAHVPEPRVIVPIRKAPSAPKPPAAPKLAVSTSNKTSQGPVPGTKKKSRPARSSEDEDEDVDEDVQVLSLAERMRRRMQVSPAPRAVAGDEDKGAPGLSSGSPVPPRGTGLPPRTAATNTVLYSEGGESEEEAVAADAYMIMSPPPKKAPAASKTKVAGGAGAVKRANKKTGGGKGGMEGEGHAKEALFSPLKGTSPAVVKKARKAAAPASKARGATKKTNKAKSGSEEEEEEEEEEEMTASPVAPRTMAPRRAAAKKVVYDMSEEEGEEEEEEEPDSEFEEEEEDDSDFEG
Sbjct:    1 MENVPKAKKKGASAKASASTAATMKAPLGESSSSINAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREAGSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELFAPGAGGYDEEEGEGGGELGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKARALDSDGDSFSEESEVEEEDDSDYAPSPKKKKGKANANAKSAMIKAHVPEPRVIVPIRKAPSAPKPPAAPKLAVSTSNKTSQGPVPGTKKKSRPARSSEDEDEDVDEDVQVLSLAERMRRRMQVSPAPRAVAGDEDKGAPGLSSGSPVPPRGTGLPPRTAATNTVLYSEGGESEEEAVAADAYMIMSPPPKKAPAASKTKVAGGAGAVKRANKKTGGGKGGMEGEGHAKEALFSPLKGTSPAVVKKARKAAAPASKARGATKKTNKAKSGSEEEEEEEEEEEMTASPVAPRTMAPRRAAAKKVVYDMSEEEGEEEEEEEPDSEFEEEEEDDSDFEG 1538          

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|635370782|emb|CCI41045.1| (unnamed protein product [Albugo candida])

HSP 1 Score: 1475.69 bits (3819), Expect = 0.000e+0
Identity = 713/1263 (56.45%), Postives = 909/1263 (71.97%), Query Frame = 0

Query:   42 RSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAP--IAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGS--KKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEE-MHPWYRGFTGTVEAKGGREAG---SYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELFAPGAGGY---------DEEEG---EGGGELGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKARALDSDGDSFSEESEVEEEDDSDYAPSPKKKKGKANANAKSAMIKAHVPEPRVIVPIRKAPSAPKPPAAPKLAVSTSNKTSQ 1284
            ++IEQIYQKKTQL+HILLRPDTY+GS + VTQ MW+ D+   K+  + I++VPGLYKIFDEI+VNA DNKQRDP+M+T+++TID  +  +SVWNNG GIPVV HKEH +YVPELIFG+LLTGSNFDD  KKTTGGRNGYGAKLANIFSTRF ++TADK   K++KQ F DNM+ +E P + S   ++  +TC+TF PDL RFK+DRLD   V+L  KR +D+AG       K L+VYLNEE+++  +F DY+ LY    A   + F++   RW+VG G SD  FQQ+SFVNGICT +GGQHV +VADQVV+KL A VKK+NKG+ VK + IKN + +YV+ALI+NP+FDSQTKETLTTRP+SFGS+ ++SDKFLK +EKS +V+ V+++AKFKQ  +L++KGG    +SK+ GITKLDDAN+AGTA   +CTLILTEGDSAK+L I GLSVVGRDYYGVFPLKGK LNVREA+H+QI+KNEEIQNIVKI+GL++G  Y   KSLRYGHLMIM DQDHDGSHIKGL+IN +HHFWPSLL++ GF+ EFITPIVK +KG  +   FYT+PEYE W+  TN   GW IKYYKGLGTST +EAKEYFSDL TH+IDF +  + DGD IDMAF+KKRVE+RK+WLRG++ GT+VDY V+ M Y  FVN+ELILFS ADN RSIP L+DG KP+QRKVL+ CFKRNLK EIKVAQLAGYVSEHS+YHHGEASL+GTI+ MAQ+F GSNN   L P+GQFGTRI+GGKDAASPRY+FT+LE +   +F P DD LL   +EDGQ+IEPE+Y PVIP VLVNG EGIGTGWSS++PNYNP D++ N+R  ++GEE    + PWYRGFTG++E +     G   ++ V G      D T+ +TELPVK WTQ YKQFLE++    +      IKD KE HTD+ V F I+++     E++     + K+ KLE+SI+ SNMHLFDA+G I KY++P A++  FY +RL+ Y KR+AA+L +L  + + + NKV+FIL+V+ G L+VSNRKK ELLQELA  GY+   P +            D EE    +G  EL KGY+YLLSM +WSLT ERVE LR +L ++  E ++L   S  +LWL D++ LEQ L   E         +    + +       + ++ KPK+R             +   EDD DY     K+  K  ++    +I            ++K  +  K    P ++ S  +K+ Q
Sbjct:   70 QTIEQIYQKKTQLQHILLRPDTYVGSIDPVTQNMWIYDEESSKMQYKKITYVPGLYKIFDEIIVNACDNKQRDPSMNTVRVTIDPLAGEVSVWNNGKGIPVVQHKEHHVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSTRFVVETADKNVRKRYKQVFSDNMSQKEAPSITSYNKQD--FTCITFRPDLKRFKLDRLDDDIVSLFKKRVFDIAGITD----KSLQVYLNEEKITCKSFPDYISLYTSDSAKEDVIFDKPTERWQVGFGPSDDGFQQISFVNGICTTRGGQHVTYVADQVVNKLVATVKKRNKGEPVKPNYIKNMMTIYVSALIDNPSFDSQTKETLTTRPASFGSAHTLSDKFLKQIEKSGIVERVLSFAKFKQTAELQRKGGKGGNRSKLTGITKLDDANYAGTAEGKNCTLILTEGDSAKSLTIGGLSVVGRDYYGVFPLKGKLLNVREASHSQILKNEEIQNIVKILGLKYGQVYQSTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLLQIEGFMHEFITPIVKCAKGRMKK-VFYTMPEYEAWRLVTNNGQGWRIKYYKGLGTSTTEEAKEYFSDLPTHQIDFSYKGEEDGDSIDMAFSKKRVEERKDWLRGYTPGTHVDYNVDDMPYSEFVNKELILFSRADNMRSIPCLVDGFKPSQRKVLYCCFKRNLKVEIKVAQLAGYVSEHSSYHHGEASLHGTIINMAQDFTGSNNTNLLSPNGQFGTRILGGKDAASPRYIFTKLESIAYKIFDPNDDSLLNFLDEDGQLIEPEWYVPVIPMVLVNGSEGIGTGWSSSIPNYNPLDLIENLRARIKGEEATRPLVPWYRGFTGSIEERSNARDGDSQAFIVRGIYHVTGDSTLVITELPVKVWTQTYKQFLETLLENET------IKDFKENHTDSKVLFTISMTPEKLQEIVQSPGGIVKKFKLETSISASNMHLFDADGRIQKYDSPHAIINAFYGIRLAYYGKRKAAMLNKLTDQIQMLANKVKFILAVISGDLIVSNRKKVELLQELADSGYDTIHPKSSKVKTTNEEQEKDSEESDDEDGTAELAKGYNYLLSMAIWSLTKERVEKLRAELKEREVEYKSLAAKSIEELWLTDLEQLEQLLHQTEE--------IRQKIKSQSIATVGNQKRQRKPKSR------------KKAVTEDDPDYGEENVKRIVKRKSSTAKELI------------VKKGSTNLKEIKLPSISTSALDKSDQ 1287          

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|698782967|ref|XP_009824527.1| (hypothetical protein, variant [Aphanomyces astaci] >gi|574120577|gb|ETV86055.1| hypothetical protein, variant [Aphanomyces astaci])

HSP 1 Score: 1473.76 bits (3814), Expect = 0.000e+0
Identity = 743/1329 (55.91%), Postives = 943/1329 (70.96%), Query Frame = 0

Query:   27 PLGESSSSINAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREAGSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYE-----LFAPGAGGYDEEEG--------EGGGELGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKL-EDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKARALDSDGDSFSEESEVEEEDDSDYAPSPKKKKGKANANAKSAMIKAHVPEPRVIVPIRKAPSA--PKPPAAPKLAVSTSNKTSQGPVPGT-KKKSRPARSSEDEDEDVDEDVQVLSLAERMRRRMQVSPAPRAVAGDED 1338
            P  +  SS +A   G ++EQIYQKKTQLEHILLRPDTY+GS E   Q +WV DD   K+VQ+ ++  PGLYKIFDEI+VNA DNKQRD TMDT+K+TID +   ISVWNNG+GIPVV+HKEH +YVPELIFG+LLTGSNFDD  KKTTGGRNGYGAKLANIFS  F ++TA +  GK+++Q F DNM+V+  P++ S   K+  +TC+TF+PD  RF+M  LD   VAL  KR YD+AG       K L VYLNEE++++ +F  Y+ LY   D  + F++ + RW+VG+G+SD  FQQVSFVNGICT KGGQHVN++ADQ+ +KL A+VKK+NKG+ VK   IKNHL +YV+ALI+NPAFDSQTKETLTTRP SFGS+ ++S+KF+K VEKS LV++++++AK KQ  +LKK  G+K  K+ GI+KLDDANFAG+A+  DCTLILTEGDSAK LA+SGL+VVGRDYYGVFPLKGK LNVREA+H+ IVKNEEIQNIVKI+GL++GT Y   KSLRYGHLMIM DQDHDGSHIKGL+IN +HHFWPSL+ L  F+QEFITPIVK +KG+ R++ FYTIPEYE W+ + N A GW IKYYKGLGTS  +EA+EYFSDL+TH+I F +  + DGD IDMAF+KKRVEDRKEWLR F  GT+VDY V+ M Y  FVN+ELILFSMADN RSIP ++DG KP+QRKVLF+CFKRNLKKEIKVAQL+GY+SEHSAYHHGE +L+GTI+GMAQNFVGSNNI  L P+GQFGTR+MGGKDAAS RYVFT LE +TR +F+P DD +L   E++GQ IEP++Y P+IP  LVNG +GIGTGWS  +PNYNP D++ N+RR ++ E    M P+YRGF G +  KGG +  ++ V G    +DD T+ ++ELPVK WTQ YKQ+LE++    +      IKD KE HTDT+V F + LS  +   +      + K+ KLESS+  SNMHLFD+ G I  Y +PL ++ EFY LRL  Y  R+AA+L+RLE +   + NK+RFIL+V++G L+V+NRKK ELL+EL ++ Y+     + AP      EEE         +  G   K YDYLLSM LWSLT ERV++LR  L  K  E + +  T+   +WL+D+D LE  L +D E  L          A+     K+AGKA  GK + +   +     ++ES+ +   D + A S  KK    +   K   IK      + +  + K P A   K   APK   + S++     V    +KK     SSEDE        +VLSLAER++RR     A + V  DED
Sbjct:   11 PTADELSSNDASGGGLTVEQIYQKKTQLEHILLRPDTYVGSIEPAEQTLWVYDDENSKMVQKKVTICPGLYKIFDEIIVNACDNKQRDSTMDTLKVTIDSEKGEISVWNNGNGIPVVMHKEHNVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSKEFVVETASREQGKRYRQVFSDNMSVKGAPKITSWSKKD--FTCITFTPDFKRFQMTGLDDDIVALFKKRVYDIAGVTD----KSLNVYLNEEKIAVKSFSQYVALYGTAD--VIFDKPDERWQVGLGLSDDGFQQVSFVNGICTTKGGQHVNYLADQITTKLIAVVKKRNKGEAVKPAYIKNHLCLYVSALIDNPAFDSQTKETLTTRPPSFGSTYTLSEKFMKAVEKSGLVENILSFAKLKQTAELKKTSGTKSVKLTGISKLDDANFAGSAKGKDCTLILTEGDSAKALAMSGLAVVGRDYYGVFPLKGKLLNVREASHSVIVKNEEIQNIVKILGLKYGTTYDSTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLMGLDNFVQEFITPIVKATKGN-RSEVFYTIPEYEAWRGQMNNAKGWYIKYYKGLGTSKPEEAREYFSDLNTHQIGFTYNGEPDGDAIDMAFSKKRVEDRKEWLRAFVPGTFVDYAVQDMPYTEFVNKELILFSMADNIRSIPSMVDGFKPSQRKVLFSCFKRNLKKEIKVAQLSGYISEHSAYHHGEVNLHGTIIGMAQNFVGSNNINLLAPNGQFGTRLMGGKDAASARYVFTNLEYITRLVFNPLDDAILNYLEDEGQSIEPQWYMPIIPMSLVNGSDGIGTGWSCGIPNYNPLDVIENLRRRIQAEPMNSMVPFYRGFKGDIVQKGGTD--NFLVQGKFEVIDDSTIVISELPVKTWTQSYKQYLETLLEANT------IKDFKENHTDTTVLFTVILSPESLAAIQNAPGGIPKKFKLESSLAISNMHLFDSNGQIKHYSSPLDIIEEFYGLRLEHYGFRKAAILKRLEHDILVLSNKMRFILAVIEGELIVNNRKKKELLEELRKQKYDPMPKNVTAPTVAAAGEEEQPAPEDDDEDNVGVSAKDYDYLLSMALWSLTAERVQNLRNDLEAKNEERDVVFATTLETMWLKDLDELEVGLGKDEENRL----------AQEHSTKKQAGKATSGKSQTKRKPAAKKKSAKESDTDSGSDFELAKSKAKKAKVVDEKPK---IKTQFGAEKPLSTVWKKPQAIVKKEGKAPKKERTASSEPKPAKVFKLFEKKQAIVESSEDE--------EVLSLAERLKRRAM---AEKKVDSDED 1298          

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|698782965|ref|XP_009824526.1| (hypothetical protein H257_02542 [Aphanomyces astaci] >gi|574120576|gb|ETV86054.1| hypothetical protein H257_02542 [Aphanomyces astaci])

HSP 1 Score: 1472.99 bits (3812), Expect = 0.000e+0
Identity = 743/1329 (55.91%), Postives = 943/1329 (70.96%), Query Frame = 0

Query:   27 PLGESSSSINAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREAGSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYE-----LFAPGAGGYDEEEG--------EGGGELGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKL-EDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKARALDSDGDSFSEESEVEEEDDSDYAPSPKKKKGKANANAKSAMIKAHVPEPRVIVPIRKAPSA--PKPPAAPKLAVSTSNKTSQGPVPGT-KKKSRPARSSEDEDEDVDEDVQVLSLAERMRRRMQVSPAPRAVAGDED 1338
            P  +  SS +A   G ++EQIYQKKTQLEHILLRPDTY+GS E   Q +WV DD   K+VQ+ ++  PGLYKIFDEI+VNA DNKQRD TMDT+K+TID +   ISVWNNG+GIPVV+HKEH +YVPELIFG+LLTGSNFDD  KKTTGGRNGYGAKLANIFS  F ++TA +  GK+++Q F DNM+V+  P++ S   K+  +TC+TF+PD  RF+M  LD   VAL  KR YD+AG       K L VYLNEE++++ +F  Y+ LY   D  + F++ + RW+VG+G+SD  FQQVSFVNGICT KGGQHVN++ADQ+ +KL A+VKK+NKG+ VK   IKNHL +YV+ALI+NPAFDSQTKETLTTRP SFGS+ ++S+KF+K VEKS LV++++++AK KQ  +LKK  G+K  K+ GI+KLDDANFAG+A+  DCTLILTEGDSAK LA+SGL+VVGRDYYGVFPLKGK LNVREA+H+ IVKNEEIQNIVKI+GL++GT Y   KSLRYGHLMIM DQDHDGSHIKGL+IN +HHFWPSL+ L  F+QEFITPIVK +KG+ R++ FYTIPEYE W+ + N A GW IKYYKGLGTS  +EA+EYFSDL+TH+I F +  + DGD IDMAF+KKRVEDRKEWLR F  GT+VDY V+ M Y  FVN+ELILFSMADN RSIP ++DG KP+QRKVLF+CFKRNLKKEIKVAQL+GY+SEHSAYHHGE +L+GTI+GMAQNFVGSNNI  L P+GQFGTR+MGGKDAAS RYVFT LE +TR +F+P DD +L   E++GQ IEP++Y P+IP  LVNG +GIGTGWS  +PNYNP D++ N+RR ++ E    M P+YRGF G +  KGG +  ++ V G    +DD T+ ++ELPVK WTQ YKQ+LE++    +      IKD KE HTDT+V F + LS  +   +      + K+ KLESS+  SNMHLFD+ G I  Y +PL ++ EFY LRL  Y  R+AA+L+RLE +   + NK+RFIL+V++G L+V+NRKK ELL+EL ++ Y+     + AP      EEE         +  G   K YDYLLSM LWSLT ERV++LR  L  K  E + +  T+   +WL+D+D LE  L +D E  L          A+     K+AGKA  GK + +   +     ++ES+ +   D + A S  KK    +   K   IK      + +  + K P A   K   APK   + S++     V    +KK     SSEDE        +VLSLAER++RR     A + V  DED
Sbjct:   11 PTADELSSNDASGGGLTVEQIYQKKTQLEHILLRPDTYVGSIEPAEQTLWVYDDENSKMVQKKVTICPGLYKIFDEIIVNACDNKQRDSTMDTLKVTIDSEKGEISVWNNGNGIPVVMHKEHNVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSKEFVVETASREQGKRYRQVFSDNMSVKGAPKITSWSKKD--FTCITFTPDFKRFQMTGLDDDIVALFKKRVYDIAGVTD----KSLNVYLNEEKIAVKSFSQYVALYGTAD--VIFDKPDERWQVGLGLSDDGFQQVSFVNGICTTKGGQHVNYLADQITTKLIAVVKKRNKGEAVKPAYIKNHLCLYVSALIDNPAFDSQTKETLTTRPPSFGSTYTLSEKFMKAVEKSGLVENILSFAKLKQTAELKKTSGTKSVKLTGISKLDDANFAGSAKGKDCTLILTEGDSAKALAMSGLAVVGRDYYGVFPLKGKLLNVREASHSVIVKNEEIQNIVKILGLKYGTTYDSTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLMGLDNFVQEFITPIVKATKGN-RSEVFYTIPEYEAWRGQMNNAKGWYIKYYKGLGTSKPEEAREYFSDLNTHQIGFTYNGEPDGDAIDMAFSKKRVEDRKEWLRAFVPGTFVDYAVQDMPYTEFVNKELILFSMADNIRSIPSMVDGFKPSQRKVLFSCFKRNLKKEIKVAQLSGYISEHSAYHHGEVNLHGTIIGMAQNFVGSNNINLLAPNGQFGTRLMGGKDAASARYVFTNLEYITRLVFNPLDDAILNYLEDEGQSIEPQWYMPIIPMSLVNGSDGIGTGWSCGIPNYNPLDVIENLRRRIQAEPMNSMVPFYRGFKGDIVQKGGTD--NFLVQGKFEVIDDSTIVISELPVKTWTQSYKQYLETLLEANT------IKDFKENHTDTTVLFTVILSPESLAAIQNAPGGIPKKFKLESSLAISNMHLFDSNGQIKHYSSPLDIIEEFYGLRLEHYGFRKAAILKRLEHDILVLSNKMRFILAVIEGELIVNNRKKKELLEELRKQKYDPMPKNVTAPTVAAAGEEEQPAPEDDDEDNVGVSAKDYDYLLSMALWSLTAERVQNLRNDLEAKNEERDVVFATTLETMWLKDLDELEVGLGKDEENRL----------AQEHSTKKQAGKATSGKSQTKRKPAAKKKSAKESDTDSGSDFELAKSKAKKAKVVDEKPK---IKTQFGAEKPLSTVWKKPQAIVKKEGKAPKKERTASSEPKPAKVFKLFEKKQAIVESSEDE--------EVLSLAERLKRRAM---AEKKVDSDED 1298          

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|298712279|emb|CBJ26730.1| (DNA topoisomerase II [Ectocarpus siliculosus])

HSP 1 Score: 1472.6 bits (3811), Expect = 0.000e+0
Identity = 718/1188 (60.44%), Postives = 899/1188 (75.67%), Query Frame = 0

Query:   27 PLGES-SSSINAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEG-EYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGIS-DGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCW-ADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREA--GSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGA------------------------FIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELFAPG------AGGYDEEEGEGGGE---------LGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYE 1169
            PLG S     N+   G+++EQ YQKKTQLEHILLRPDTYIGSTE ++Q MWV D+    +V+R ++FVPGLYKIFDEI+VNA+DNKQRD  M  +K+T+D +S  ISVWN+G+GIP+ +HKEH IYVPELIFGNLLTGSNFDD  KKTTGGRNG+GAKLANI+ST F ++TAD+ +GK ++Q FR+NM  +E PE+      EG ++TC+TF+PD  RF M  ++  T+ALL KR YD+AGC  G+ G  +KVYL+  R+ +  F DY+ +YEG++ P+ FE +  RWEVGV +S D  FQQVS+VN ICTVKGG HV  VA+QV++KL  ++K+K+K  EVKGH +KNHL V+VN L+ENPAF+SQ+KE LT++ + FGS   +SD  LK +EKS +VD V++WAK KQ++QL KK G K SK+ GITKLDDAN AGTA+S DCTLILTEGDSAKTLA+SGL VVGRD YGVFPLKGK LNVREA   Q++KNEEIQN+VKI+GL+F   Y D K LRYGHLMIMTDQDHDGSHIKGLLIN +HH+WPSLLK+ GFLQEFITPIVKVSKG     +FYT+PEY  WKE      GW  KYYKGLGTSTA+EAKEYF+ L TH+++F W  DD D D+IDMAF+KKRVEDRKEWLRGF  GT+ DY VES++Y++F+N+ELILFSM DN+RSIP L+DG KP+QRKVLF+CFKRNLKKEIKV QLAGYVSEHSAYHHGE SLNGTI  MAQNF GSNN+  L P GQFGTR+MGGKDAASPRY+FT L++  RA+FHP+DD LL   ++DG  IEPE+Y P+IP +LVNG +GIGTGWSS VPNY+P D++ N++R ++G++  EM PWY+GF G++EAK G++   GSY V G   R+DD TVE+TELPV+KWTQDYKQFLES+  GA D K                           IKD KE HTDT+V+F +T+S      L   D  L+K  KLESS++T+NM LFD +GH+ +YE+P  ++ EF  LR   Y +R+ +LL +L+ EW ++DNKVRFIL+VV+ R+ V+NRKK+ELL++L REGY+LF P       AGG ++ EGE G E         L KGYDYLL MK+WSLTLERV+ L  +L  KA E+  L+ T+ ++LWL+D+DAL   L++ +
Sbjct:   24 PLGSSRGGGANSNGGGQTVEQRYQKKTQLEHILLRPDTYIGSTEKMSQEMWVYDEVADSIVKRAVTFVPGLYKIFDEIIVNAADNKQRDENMSKLKVTVDAESGKISVWNDGAGIPIQMHKEHHIYVPELIFGNLLTGSNFDDDQKKTTGGRNGFGAKLANIYSTEFVVETADRDAGKHYRQVFRNNMQEKEAPEIDC--DYEGPDFTCITFTPDWKRFHMTGMEADTIALLRKRVYDMAGCMGGYAGASVKVYLDNRRVKVSGFKDYMSMYEGMETPVIFESIGDRWEVGVAVSADSVFQQVSYVNAICTVKGGTHVECVANQVINKLLPVLKRKHKA-EVKGHFVKNHLCVFVNGLVENPAFNSQSKEALTSKDTLFGSVPKISDAGLKKLEKSEIVDRVLSWAKHKQNQQLNKKSGKKSSKLTGITKLDDANHAGTAKSQDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLKGKLLNVREATGPQVLKNEEIQNVVKILGLKFNHTYDDTKQLRYGHLMIMTDQDHDGSHIKGLLINFVHHYWPSLLKIDGFLQEFITPIVKVSKGRNEL-SFYTVPEYTVWKEGHANGKGWNAKYYKGLGTSTAKEAKEYFAALDTHQLEFEWQDDDRDDDLIDMAFSKKRVEDRKEWLRGFKAGTHADYNVESVTYESFINKELILFSMEDNKRSIPCLVDGFKPSQRKVLFSCFKRNLKKEIKVVQLAGYVSEHSAYHHGETSLNGTITAMAQNFTGSNNLNLLTPGGQFGTRLMGGKDAASPRYIFTALQKAARAVFHPDDDALLTYLDDDGLSIEPEFYMPIIPLILVNGSDGIGTGWSSNVPNYSPHDVIENLKRRIKGDKFVEMQPWYKGFAGSIEAKVGKKGFEGSYTVRGIANRIDDTTVEITELPVRKWTQDYKQFLESLLPGAEDAKSKDAKGNGKGKGKGKGAADKDKSPTRCIKDFKENHTDTTVHFTVTMSMEDLDALEKGD-GLYKFFKLESSVSTNNMVLFDQDGHLERYESPQHIMEEFITLRSEFYGRRKTSLLSKLQSEWSKLDNKVRFILAVVEDRMQVNNRKKSELLEQLKREGYDLFLPTTSKLRVAGGAEDGEGEDGNEESAADDSDTLSKGYDYLLGMKIWSLTLERVKLLENELEGKAEEMRILKATAPSELWLKDLDALGDVLDEID 1206          

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|635370783|emb|CCI41046.1| (unnamed protein product [Albugo candida])

HSP 1 Score: 1470.68 bits (3806), Expect = 0.000e+0
Identity = 713/1265 (56.36%), Postives = 909/1265 (71.86%), Query Frame = 0

Query:   42 RSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAP--IAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQ--TKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGS--KKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEE-MHPWYRGFTGTVEAKGGREAG---SYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELFAPGAGGY---------DEEEG---EGGGELGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKARALDSDGDSFSEESEVEEEDDSDYAPSPKKKKGKANANAKSAMIKAHVPEPRVIVPIRKAPSAPKPPAAPKLAVSTSNKTSQ 1284
            ++IEQIYQKKTQL+HILLRPDTY+GS + VTQ MW+ D+   K+  + I++VPGLYKIFDEI+VNA DNKQRDP+M+T+++TID  +  +SVWNNG GIPVV HKEH +YVPELIFG+LLTGSNFDD  KKTTGGRNGYGAKLANIFSTRF ++TADK   K++KQ F DNM+ +E P + S   ++  +TC+TF PDL RFK+DRLD   V+L  KR +D+AG       K L+VYLNEE+++  +F DY+ LY    A   + F++   RW+VG G SD  FQQ+SFVNGICT +GGQHV +VADQVV+KL A VKK+NKG+ VK + IKN + +YV+ALI+NP+FDSQ  TKETLTTRP+SFGS+ ++SDKFLK +EKS +V+ V+++AKFKQ  +L++KGG    +SK+ GITKLDDAN+AGTA   +CTLILTEGDSAK+L I GLSVVGRDYYGVFPLKGK LNVREA+H+QI+KNEEIQNIVKI+GL++G  Y   KSLRYGHLMIM DQDHDGSHIKGL+IN +HHFWPSLL++ GF+ EFITPIVK +KG  +   FYT+PEYE W+  TN   GW IKYYKGLGTST +EAKEYFSDL TH+IDF +  + DGD IDMAF+KKRVE+RK+WLRG++ GT+VDY V+ M Y  FVN+ELILFS ADN RSIP L+DG KP+QRKVL+ CFKRNLK EIKVAQLAGYVSEHS+YHHGEASL+GTI+ MAQ+F GSNN   L P+GQFGTRI+GGKDAASPRY+FT+LE +   +F P DD LL   +EDGQ+IEPE+Y PVIP VLVNG EGIGTGWSS++PNYNP D++ N+R  ++GEE    + PWYRGFTG++E +     G   ++ V G      D T+ +TELPVK WTQ YKQFLE++    +      IKD KE HTD+ V F I+++     E++     + K+ KLE+SI+ SNMHLFDA+G I KY++P A++  FY +RL+ Y KR+AA+L +L  + + + NKV+FIL+V+ G L+VSNRKK ELLQELA  GY+   P +            D EE    +G  EL KGY+YLLSM +WSLT ERVE LR +L ++  E ++L   S  +LWL D++ LEQ L   E         +    + +       + ++ KPK+R             +   EDD DY     K+  K  ++    +I            ++K  +  K    P ++ S  +K+ Q
Sbjct:   70 QTIEQIYQKKTQLQHILLRPDTYVGSIDPVTQNMWIYDEESSKMQYKKITYVPGLYKIFDEIIVNACDNKQRDPSMNTVRVTIDPLAGEVSVWNNGKGIPVVQHKEHHVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSTRFVVETADKNVRKRYKQVFSDNMSQKEAPSITSYNKQD--FTCITFRPDLKRFKLDRLDDDIVSLFKKRVFDIAGITD----KSLQVYLNEEKITCKSFPDYISLYTSDSAKEDVIFDKPTERWQVGFGPSDDGFQQISFVNGICTTRGGQHVTYVADQVVNKLVATVKKRNKGEPVKPNYIKNMMTIYVSALIDNPSFDSQVSTKETLTTRPASFGSAHTLSDKFLKQIEKSGIVERVLSFAKFKQTAELQRKGGKGGNRSKLTGITKLDDANYAGTAEGKNCTLILTEGDSAKSLTIGGLSVVGRDYYGVFPLKGKLLNVREASHSQILKNEEIQNIVKILGLKYGQVYQSTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLLQIEGFMHEFITPIVKCAKGRMKK-VFYTMPEYEAWRLVTNNGQGWRIKYYKGLGTSTTEEAKEYFSDLPTHQIDFSYKGEEDGDSIDMAFSKKRVEERKDWLRGYTPGTHVDYNVDDMPYSEFVNKELILFSRADNMRSIPCLVDGFKPSQRKVLYCCFKRNLKVEIKVAQLAGYVSEHSSYHHGEASLHGTIINMAQDFTGSNNTNLLSPNGQFGTRILGGKDAASPRYIFTKLESIAYKIFDPNDDSLLNFLDEDGQLIEPEWYVPVIPMVLVNGSEGIGTGWSSSIPNYNPLDLIENLRARIKGEEATRPLVPWYRGFTGSIEERSNARDGDSQAFIVRGIYHVTGDSTLVITELPVKVWTQTYKQFLETLLENET------IKDFKENHTDSKVLFTISMTPEKLQEIVQSPGGIVKKFKLETSISASNMHLFDADGRIQKYDSPHAIINAFYGIRLAYYGKRKAAMLNKLTDQIQMLANKVKFILAVISGDLIVSNRKKVELLQELADSGYDTIHPKSSKVKTTNEEQEKDSEESDDEDGTAELAKGYNYLLSMAIWSLTKERVEKLRAELKEREVEYKSLAAKSIEELWLTDLEQLEQLLHQTEE--------IRQKIKSQSIATVGNQKRQRKPKSR------------KKAVTEDDPDYGEENVKRIVKRKSSTAKELI------------VKKGSTNLKEIKLPSISTSALDKSDQ 1289          

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|1173949142|gb|OQR97211.1| (DNA topoisomerase II [Thraustotheca clavata])

HSP 1 Score: 1470.29 bits (3805), Expect = 0.000e+0
Identity = 748/1350 (55.41%), Postives = 953/1350 (70.59%), Query Frame = 0

Query:   30 ESSSSINAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVL-GITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREAGSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELFAPGAGGYDEEEGEGG------GELGKG-YDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKARALDSDGDSFSEESEVEEEDDSDYA-------------PSPKKKK-------------GKANANAKSAMIKAHVPEPRVIVPIRKAPSAPKPPAAPKLAVSTSNKTSQGPVPGTKKKSRPARSSEDEDEDVDEDVQVLSLAERMRRRMQVSPAPRAVAGDEDKGAPGLS 1345
            E SS  ++   G ++EQIYQKKTQLEHILLRPDTY+GS E VTQ MWV D+ +KK+V + I+F PGLYKIFDEI+VNA DNKQRD +MDT+K+T DQ++  ISVWNNG GIPVV HKEH++YVPELIFG+LLTGSNFDD  KKTTGGRNGYGAKLANIFST F ++TA++  GK+++Q F+ NM+V+EEP++ S   K+  +TC+TF+PD TRF+M  LD   +AL  KR YD+AG       K L VYLNEE++ + TF  Y+++Y   +  + F++ + RW VG+GISD  FQQVSFVNGICT KGGQHVN++ADQ+ +KL+A +KKKNKG  VK   IKNHL +Y+ ALI+NPAFDSQTKETLTTR ++FGS+ ++SDKFLK VEKSSLV+ ++++AKFKQ   LKK  G  K K L GI+KLDDANFAG+A+S  CTLILTEGDSAK LA+SGLSVVGRDYYGVFPL+GK LNVREAAH+ IVKNEE+ NIVKI+GL +  +Y   K+LRYGHLMIM DQDHDGSHIKGL+IN +H++WPSLL L  F+QEFITPI+K +KG+ R   FYT+PEYE W++ TN   GW IKYYKGLGTSTA+EAKEYFSDL+TH+I F +  + D D I++AF+KKR+EDRKEWLR F  GTY+DY +E M Y  FVN+ELILFSMADN RSIP ++DG KP+QRKVLF+CFKRNLKKEIKVAQLAGYVSEHSAYHHGEASL+GTI+GMAQ++VGSNN+  L P+GQFGTR+ GGKDAAS RYVFT+LE +TR ++HP DD +L   EE+G  IEP++Y P+IP  L+NG EGIGTGWS  +PNYNP +++ANIRR ++ E    M PWYRGFTG++  KG   + ++ V G    VD+ T+ ++ELP+K WTQ YK+ LE M + ++D     IKD KE HTDT+V F I+L       +  +   + K+ KLESS+ TSNMH+F+AEG I  Y++PL V+ EFY LR+  Y +R+ A+L++L+ E   + NK+RFIL+V+DG LVV+NRKK +LL+EL R  Y+     A     +E +        GE+  G YDYLLSM LWSLT ERVE LR  L  K AE + + GTS  D+WL D++ LE+ LE +E     +     A+A  K KG  + +A   K  A+   +  D  + ES+ E +     A             P+ KK+K             GK       +   A   EP+   P+++   APK P APK A            P   KK    +  ED     D+D +VLSL ER++RR +   A       + K AP +S
Sbjct:   18 EFSSDTSSTRSGLTVEQIYQKKTQLEHILLRPDTYVGSIEPVTQNMWVFDEEEKKMVNKKITFCPGLYKIFDEIIVNACDNKQRDKSMDTLKVTFDQETGAISVWNNGRGIPVVEHKEHKVYVPELIFGHLLTGSNFDDNKKKTTGGRNGYGAKLANIFSTEFIVETANREDGKRYRQVFKKNMSVKEEPKITSWSKKD--FTCITFTPDFTRFQMTGLDSDIIALFKKRVYDIAGVTD----KSLSVYLNEEKIDVKTFPQYVEMYNPQE--VVFDKPDERWHVGIGISDDGFQQVSFVNGICTTKGGQHVNYIADQIATKLSATIKKKNKGDPVKPALIKNHLFIYMTALIDNPAFDSQTKETLTTRANAFGSTFTLSDKFLKQVEKSSLVERILSFAKFKQTADLKKAAGGGKKKRLQGISKLDDANFAGSAKSHMCTLILTEGDSAKALAMSGLSVVGRDYYGVFPLRGKLLNVREAAHSAIVKNEEVTNIVKILGLEYNKKYESTKNLRYGHLMIMADQDHDGSHIKGLVINFIHNYWPSLLGLESFVQEFITPIIKATKGN-RVQVFYTMPEYETWRQSTNNGRGWDIKYYKGLGTSTAKEAKEYFSDLNTHQIAFTYEKEKDDDAIELAFSKKRIEDRKEWLRAFVPGTYIDYSMEDMGYSDFVNKELILFSMADNIRSIPSMVDGFKPSQRKVLFSCFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLHGTIIGMAQDYVGSNNLNLLSPNGQFGTRLQGGKDAASARYVFTKLENLTRLVYHPLDDSVLNYLEEEGLSIEPDFYMPIIPMALINGNEGIGTGWSCGIPNYNPNEVIANIRRRIQAEPMTPMQPWYRGFTGSIVQKGT--SDNFLVQGKFEVVDEGTIVISELPIKTWTQTYKERLEKM-IESND-----IKDFKENHTDTTVLFTISLEPAKLAAIQQESGGILKKFKLESSMATSNMHMFNAEGKIKLYKSPLEVIEEFYTLRIDYYGRRKVAILKKLDTEIMALSNKMRFILAVIDGSLVVNNRKKKDLLEELRRLKYDPMPKKAAAKSSDEEDIIDEEEPQGEVSAGDYDYLLSMPLWSLTAERVEILRNDLHSKEAERDTVFGTSLEDMWLHDLEVLEKNLEIHEENRLAQE----ASALKKFKGAASKRAPAKKKAAKRKANGSDDENSESDFEVKVPKVKAPPKKKAATGEGAKPAMKKEKPIKAEKAAKSDTAGKKMTQTTLSAFSAVKDEPK---PVKEEKPAPK-PRAPKKA------------PAQPKKLLELKRKEDSS---DDDGEVLSLFERLQRRKEADAA-------KSKAAPSIS 1320          

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|673019868|ref|XP_008863895.1| (hypothetical protein H310_02231 [Aphanomyces invadans] >gi|574480809|gb|ETW07802.1| hypothetical protein H310_02231 [Aphanomyces invadans])

HSP 1 Score: 1467.6 bits (3798), Expect = 0.000e+0
Identity = 791/1539 (51.40%), Postives = 1028/1539 (66.80%), Query Frame = 0

Query:   28 LGESSSSINAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREAGSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELF-----AP--GAGGYD----EEEGEGGGEL-GKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKAR---------ALDSDGDSFSE----ESEVEEEDDSDYAPSPKKKKGKANANAKSAMIKAHVPEPRVIVPIRKAPSAPKPPAAPKLAVSTSNKTSQGPVPGTKKK--SRPARSSEDEDEDVDEDVQVLSLAERMRRRMQVSPAPRAVAGDEDKGAPGLSSGSPVPPRGTGLPPRTAATNTVLYSEGGE------SEEEAVAADAYMIMSPPPKKAPAASKT-------KVAGGAGAVKRANKKTGGGKGGMEGEGHAKEALFSPLKGTSPAVVKKARKA-AAPASKARGATKKTNKAKSGSEEEEEEEEEEEMTASPVAPRTMAPRRAAAKKVVY--DMSEEEGEEEEEEEPD 1523
            L ++  + N G  G +IEQIYQKKTQLEHILLRPDTY+GS E   Q +WV DD   K+VQ+ ++  PGLYKIFDEI+VNA DNKQRD TMDT+K+TID +   ISVWNNG+GIPVV+H+EH +YVPELIFG+LLTGSNFDD  KKTTGGRNGYGAKLANIFS  F ++TA +  GK+++Q F +NM+V+  P++ S   K+  +TC+TF+PD  RF+M  LD   VAL  KR YD+AG       K L VYLNEE++++ +F  Y+ LY   D  + + + + RW+VG+G+SD  FQQVSFVNGICT KGGQHVN++ADQ+ +KL  +VKK+NKG+ VK   IKNHL +YV+ALI+NPAFDSQTKETLTTR  +FGS+ ++SDKF+K VEKS LV++++++AK KQ   LKK  G+K  K+ GI+KLDDANFAG+A+  DCTLILTEGDSAK LA+SGL+VVGRDYYGVFPLKGK LNVREA+H+ IVKNEEIQNIVKI+GL++GT Y   KSLRYGHLMIM DQDHDGSHIKGL+IN +HHFWPSL+ L  F+QEFITPIVK +KG+ R++ FYTIPEYE W+ R  +  GW IKYYKGLGTS  ++A+EYFSDL+TH+I F +  + DGD IDMAF+KKRVEDRK+WLR F  GTYVDY V+ M Y  FVN+ELILFSMADN RSIP ++DG KP+QRKVLF+CFKRNLKKEIKVAQLAGY+SEHSAYHHGE +L+GTI+GMAQNFVGSNNI  L P+GQFGTR+MGGKDAAS RYVFT +E VTR +FHP DD +L   E++GQ IEP++Y P IP  LVNG +GIGTGWS  +PNYNP DI+ N+RR ++ E  + M P+YRGF G +  KGG +  ++ V G    VD+ T+ ++ELPVK WTQ YKQFLE++    +      IKD KE HTDT+V F + LS  +   +      + K+ KLESS+  SNMHLFD+ G I  Y +PL ++ +FY LRL  Y  R+AA+L+RLE +   + NK+RFIL+V++G LVV+NRKK +LL+EL  + ++       AP   A G D    EE+ +   E+  K YDYLLS+ +WSLT ERV+ LR  L +K  E + +  T+   +WL+D+  LE  L   E         +   A+ +   K+AGKAK GK +A+          LDSD DS S+    +++V+++ D D  P  +K    A      A+ K  V  P     ++K   APK       A ++S+K ++      KK   S  A SSEDE        +VLSL+ER++RR     A +    DED      SS + +  +   LP  +  ++  +++  G         ++  A D    ++  PK A  A+ T       K+A  A +V+ ++K         E     K A          A     RK    P S  +   KK N +   S +  + E++++    PVAPR    R  A K+VVY  D S++E EE E +E D
Sbjct:    4 LTKADGTNNGG--GLTIEQIYQKKTQLEHILLRPDTYVGSIEPAEQSLWVFDDENSKMVQKKVTICPGLYKIFDEIIVNACDNKQRDSTMDTLKVTIDAEKGEISVWNNGNGIPVVMHQEHNVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSKEFIVETASREQGKRYRQVFSNNMSVKGAPKITSWSKKD--FTCITFTPDFERFQMTGLDDDIVALFKKRVYDIAGITD----KSLNVYLNEEKIAVKSFPQYVALYGTPD--VIYGQPDERWQVGLGLSDDGFQQVSFVNGICTTKGGQHVNYIADQITNKLVGIVKKRNKGEAVKPAYIKNHLCLYVSALIDNPAFDSQTKETLTTRSPAFGSTYTLSDKFMKAVEKSGLVENILSFAKLKQTAALKKTSGTKSVKLTGISKLDDANFAGSAKGKDCTLILTEGDSAKALAMSGLAVVGRDYYGVFPLKGKLLNVREASHSVIVKNEEIQNIVKILGLKYGTTYESTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLMGLDNFVQEFITPIVKATKGN-RSEVFYTIPEYEAWRNRMVDTKGWYIKYYKGLGTSKPEDAREYFSDLNTHQIGFTYNGEADGDAIDMAFSKKRVEDRKDWLRAFVPGTYVDYAVQDMPYTEFVNKELILFSMADNIRSIPSMVDGFKPSQRKVLFSCFKRNLKKEIKVAQLAGYISEHSAYHHGEVNLHGTIIGMAQNFVGSNNINLLSPNGQFGTRLMGGKDAASARYVFTNVESVTRLVFHPLDDPILNYLEDEGQSIEPQWYMPTIPMSLVNGSDGIGTGWSCGIPNYNPLDIIENLRRRIQAEPMKSMVPFYRGFKGEIVQKGGSD--NFLVQGRYEIVDESTIVISELPVKTWTQSYKQFLETLLDANT------IKDFKENHTDTAVLFTVVLSPESLAAIQQAPGGIMKKFKLESSLAISNMHLFDSNGQIKHYSSPLDIIEDFYGLRLEYYGYRKAAILKRLEHDIMILSNKMRFILAVIEGELVVNNRKKQDLLEELRMKKFDPMPRKSTAPTVAAAGEDDPVAEEDDDSHSEVTAKDYDYLLSLPMWSLTAERVQSLRDDLEEKNQERDTVFSTTLESMWLKDLHELELGLSKEE---------MKRLAQEESTMKQAGKAKSGKSQAKRKPPAKKRTHLDSDNDSGSDFELTKAKVKKQKDDDEKPKIEK---LAVVKPPVAVWKNPVEGPPAQTKVKKISKAPKKELN---ATASSDKPTKLFKLFEKKPAVSVAAESSEDE--------EVLSLSERLKRRAM---AEKNAESDEDDVFNAPSSAAQI-MKTEVLPGVSVKSDDNVFAFAGSLKKAASKAKKTPATDKPKKVAAKPKVAKTATNTTEMPVKPKLAKKAVSVE-SDKDDTFNFSDAESVPAVKTAASKTKSSKPAASSAAKRKTEKTPGSPKQKKLKKPNWSDEESSDSGDGEDDKD---EPVAPRERHGR--ATKQVVYKADSSDDEQEESEFDESD 1490          

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|325187394|emb|CCA21933.1| (predicted protein putative [Albugo laibachii Nc14] >gi|325190307|emb|CCA24783.1| topoisomerase putative [Albugo laibachii Nc14])

HSP 1 Score: 1466.83 bits (3796), Expect = 0.000e+0
Identity = 707/1200 (58.92%), Postives = 900/1200 (75.00%), Query Frame = 0

Query:   34 SINAGEEGRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAP--IAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKG--GSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEE-MHPWYRGFTGTVEAKGGREAG---SYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYE-LFAPGAGGYDE---------EEGEGGGELGKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKARALDSDGDSFSEE 1215
            S NA ++ ++IEQIYQKKTQL+HILLRPDTY+GS + VTQ MW+ D+   K+  + I+FVPGLYKIFDEI+VNA DNKQRD +M+T+++TID  +  ISVWNNG GIPVV HKEH++YVPELIFG+LLTGSNFDD  KKTTGGRNGYGAKLANIFST+F ++TADK   K++KQ F DNM+ +E P + S   ++  +TC+TF PDL RFKMDRLD   V+L  KR +D+AG       K L+VYLNEE+++  +F DY+ LY G  A   + F++   RW+VG G SD  FQQ+SFVNGICT +GGQHV ++ADQVV+KL A VKK+NKG+ VK + IKN + +YV+ALI+NPAFDSQTKETLTTRPSSFGS+ S+SDKFLK +EKS +V+ VV++AKFKQ  +L++KG  G  +SK+ GITKLDDAN+AGTA    CTLILTEGDSAK+L I GLSVVGRDYYGVFPLKGK LNVREA+H+QI+KNEEIQN+VKI+GL++G  Y   KSLRYGHLMIM DQDHDGSHIKGL+IN +HHFWPSLL++ GF+ EFITPIVK +KGSK+ + FYT+PEYE W+  TN   GW IKYYKGLGTST +EAKEYFSDL+TH+IDF +  + DGD IDMAF+KKRVE+RK WLRG++ GT+VDY +  M Y  FVN+ELILFS ADN RSIP L+DG KP+QRKVL+ CFKRNL+ EIKVAQLAGYVSEHS+YHHGEASL+GTI+ MAQ+F+GSNN   L P+GQFGTRI+GGKDAASPRY+FT+LE +    F P D+ LL   +EDGQ+IEPE+Y PVIP VLVNG EGIGTGWSS +PNYNP D++ N+R  ++GE P + + PWYRGFTG++E + G   G   ++ V G     DD T+ +TELPVK WT  YKQFLES+    +      IKD KE HTD+ V F I+++     E+++    + ++ KLESSI+ +NMHLFDA+G I KY++P  ++  FY +RL+ Y KR+AA+LQ+L  +   + NKV+FIL+V+ G L+VSNRKK ELL EL   GY+ L+   + G +          E  E  G+L KGY+YLLSMK+WSLT ERV+ LR +L ++  E ++L   S  +LWL D+D LE+ L + E   +   A  +A  +   K ++    +K  P+ +A+  D   + E+
Sbjct:   63 SKNASQK-QTIEQIYQKKTQLQHILLRPDTYVGSIDPVTQNMWIYDEEASKMHLKKITFVPGLYKIFDEIIVNACDNKQRDASMNTLRVTIDPTAGEISVWNNGKGIPVVQHKEHEVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSTKFIVETADKNVKKRYKQVFSDNMSQKESPAITSWNKQD--FTCITFRPDLKRFKMDRLDDDIVSLFKKRVFDIAGVTD----KSLQVYLNEEKITCKSFPDYISLYTGESAKEDVIFDKPTERWQVGFGPSDDGFQQISFVNGICTTRGGQHVTYIADQVVNKLVAAVKKRNKGEAVKPNYIKNMMTIYVSALIDNPAFDSQTKETLTTRPSSFGSTHSLSDKFLKQIEKSGIVERVVSFAKFKQTAELQRKGAKGGNRSKLTGITKLDDANYAGTAEGRKCTLILTEGDSAKSLTIGGLSVVGRDYYGVFPLKGKLLNVREASHSQILKNEEIQNVVKILGLKYGQVYESTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLLQVEGFMHEFITPIVKCAKGSKK-EVFYTMPEYEAWRLATNNGQGWRIKYYKGLGTSTTEEAKEYFSDLATHQIDFSYKGEEDGDSIDMAFSKKRVEERKNWLRGYTPGTHVDYNIADMPYSEFVNKELILFSRADNMRSIPCLVDGFKPSQRKVLYCCFKRNLRVEIKVAQLAGYVSEHSSYHHGEASLHGTIINMAQDFIGSNNSNLLSPNGQFGTRILGGKDAASPRYIFTKLEAIAYKFFDPNDNSLLNYLDEDGQLIEPEWYVPVIPMVLVNGSEGIGTGWSSFIPNYNPLDLIENLRARIKGEAPTKPLVPWYRGFTGSIEERSGTRDGDSQAFVVRGIFHVEDDSTLVITELPVKTWTHTYKQFLESLMENDT------IKDFKENHTDSKVLFTISMTPEKMQEIVLSPGGIMRKFKLESSISAANMHLFDADGRIQKYDSPHEIISAFYDIRLAYYGKRKAAMLQKLTDQIEMLANKVKFILAVIQGDLIVSNRKKVELLHELVEMGYDKLYPNNSKGKNSDEDEEKDDQEGDEEEGDLTKGYNYLLSMKIWSLTKERVDKLRAELKEREVEYKSLEAKSIEELWLTDLDTLEELLHETEETRQRINAQSIATVQNGSKKRQ----RKQIPRKKAIKEDDSEYKED 1244          

BLAST of EWM24585.1 vs. NCBI_GenBank
Match: gi|673019870|ref|XP_008863896.1| (hypothetical protein, variant [Aphanomyces invadans] >gi|574480810|gb|ETW07803.1| hypothetical protein, variant [Aphanomyces invadans])

HSP 1 Score: 1465.29 bits (3792), Expect = 0.000e+0
Identity = 739/1325 (55.77%), Postives = 942/1325 (71.09%), Query Frame = 0

Query:   41 GRSIEQIYQKKTQLEHILLRPDTYIGSTESVTQPMWVLDDAQKKLVQRNISFVPGLYKIFDEIMVNASDNKQRDPTMDTIKITIDQDSNTISVWNNGSGIPVVVHKEHQIYVPELIFGNLLTGSNFDDGMKKTTGGRNGYGAKLANIFSTRFTIQTADKASGKKFKQTFRDNMTVREEPEVVSMGSKEGEYTCVTFSPDLTRFKMDRLDGATVALLAKRAYDVAGCASGFPGKPLKVYLNEERLSLPTFLDYLKLYEGLDAPIAFERVNPRWEVGVGISDGQFQQVSFVNGICTVKGGQHVNFVADQVVSKLTALVKKKNKGQEVKGHQIKNHLVVYVNALIENPAFDSQTKETLTTRPSSFGSSCSVSDKFLKTVEKSSLVDSVVNWAKFKQDEQLKKKGGSKKSKVLGITKLDDANFAGTARSSDCTLILTEGDSAKTLAISGLSVVGRDYYGVFPLKGKPLNVREAAHAQIVKNEEIQNIVKIMGLRFGTQYTDAKSLRYGHLMIMTDQDHDGSHIKGLLINLLHHFWPSLLKLPGFLQEFITPIVKVSKGSKRADAFYTIPEYEQWKERTNEAAGWTIKYYKGLGTSTAQEAKEYFSDLSTHRIDFCWADDGDGDVIDMAFAKKRVEDRKEWLRGFSKGTYVDYKVESMSYDTFVNQELILFSMADNQRSIPHLMDGLKPAQRKVLFACFKRNLKKEIKVAQLAGYVSEHSAYHHGEASLNGTIVGMAQNFVGSNNIGFLYPSGQFGTRIMGGKDAASPRYVFTRLEEVTRALFHPEDDQLLPLQEEDGQVIEPEYYAPVIPTVLVNGGEGIGTGWSSTVPNYNPRDIMANIRRLLRGEEPEEMHPWYRGFTGTVEAKGGREAGSYAVLGTVRRVDDVTVEVTELPVKKWTQDYKQFLESMTVGASDEKGAFIKDLKERHTDTSVYFVITLSETAAPELLVDDQALFKRLKLESSITTSNMHLFDAEGHIVKYETPLAVLREFYPLRLSLYEKRRAALLQRLEREWRRMDNKVRFILSVVDGRLVVSNRKKAELLQELAREGYELF-----AP--GAGGYD----EEEGEGGGEL-GKGYDYLLSMKLWSLTLERVEDLRGQLAQKAAELEALRGTSAADLWLQDMDALEQKLEDYEAGLEFEPAYVVAAARGKGKGKKAGKAKKGKPKAR---------ALDSDGDSFSE----ESEVEEEDDSDYAPSPKKKKGKANANAKSAMIKAHVPEPRVIVPIRKAPSAPKPPAAPKLAVSTSNKTSQGPVPGTKKK--SRPARSSEDEDEDVDEDVQVLSLAERMRRRMQVSPAPRAVAGDED 1338
            G +IEQIYQKKTQLEHILLRPDTY+GS E   Q +WV DD   K+VQ+ ++  PGLYKIFDEI+VNA DNKQRD TMDT+K+TID +   ISVWNNG+GIPVV+H+EH +YVPELIFG+LLTGSNFDD  KKTTGGRNGYGAKLANIFS  F ++TA +  GK+++Q F +NM+V+  P++ S   K+  +TC+TF+PD  RF+M  LD   VAL  KR YD+AG       K L VYLNEE++++ +F  Y+ LY   D  + + + + RW+VG+G+SD  FQQVSFVNGICT KGGQHVN++ADQ+ +KL  +VKK+NKG+ VK   IKNHL +YV+ALI+NPAFDSQTKETLTTR  +FGS+ ++SDKF+K VEKS LV++++++AK KQ   LKK  G+K  K+ GI+KLDDANFAG+A+  DCTLILTEGDSAK LA+SGL+VVGRDYYGVFPLKGK LNVREA+H+ IVKNEEIQNIVKI+GL++GT Y   KSLRYGHLMIM DQDHDGSHIKGL+IN +HHFWPSL+ L  F+QEFITPIVK +KG+ R++ FYTIPEYE W+ R  +  GW IKYYKGLGTS  ++A+EYFSDL+TH+I F +  + DGD IDMAF+KKRVEDRK+WLR F  GTYVDY V+ M Y  FVN+ELILFSMADN RSIP ++DG KP+QRKVLF+CFKRNLKKEIKVAQLAGY+SEHSAYHHGE +L+GTI+GMAQNFVGSNNI  L P+GQFGTR+MGGKDAAS RYVFT +E VTR +FHP DD +L   E++GQ IEP++Y P IP  LVNG +GIGTGWS  +PNYNP DI+ N+RR ++ E  + M P+YRGF G +  KGG +  ++ V G    VD+ T+ ++ELPVK WTQ YKQFLE++    +      IKD KE HTDT+V F + LS  +   +      + K+ KLESS+  SNMHLFD+ G I  Y +PL ++ +FY LRL  Y  R+AA+L+RLE +   + NK+RFIL+V++G LVV+NRKK +LL+EL  + ++       AP   A G D    EE+ +   E+  K YDYLLS+ +WSLT ERV+ LR  L +K  E + +  T+   +WL+D+  LE  L   E         +   A+ +   K+AGKAK GK +A+          LDSD DS S+    +++V+++ D D  P  +K    A      A+ K  V  P     ++K   APK       A ++S+K ++      KK   S  A SSEDE        +VLSL+ER++RR     A +    DED
Sbjct:   15 GLTIEQIYQKKTQLEHILLRPDTYVGSIEPAEQSLWVFDDENSKMVQKKVTICPGLYKIFDEIIVNACDNKQRDSTMDTLKVTIDAEKGEISVWNNGNGIPVVMHQEHNVYVPELIFGHLLTGSNFDDKKKKTTGGRNGYGAKLANIFSKEFIVETASREQGKRYRQVFSNNMSVKGAPKITSWSKKD--FTCITFTPDFERFQMTGLDDDIVALFKKRVYDIAGITD----KSLNVYLNEEKIAVKSFPQYVALYGTPD--VIYGQPDERWQVGLGLSDDGFQQVSFVNGICTTKGGQHVNYIADQITNKLVGIVKKRNKGEAVKPAYIKNHLCLYVSALIDNPAFDSQTKETLTTRSPAFGSTYTLSDKFMKAVEKSGLVENILSFAKLKQTAALKKTSGTKSVKLTGISKLDDANFAGSAKGKDCTLILTEGDSAKALAMSGLAVVGRDYYGVFPLKGKLLNVREASHSVIVKNEEIQNIVKILGLKYGTTYESTKSLRYGHLMIMADQDHDGSHIKGLVINFIHHFWPSLMGLDNFVQEFITPIVKATKGN-RSEVFYTIPEYEAWRNRMVDTKGWYIKYYKGLGTSKPEDAREYFSDLNTHQIGFTYNGEADGDAIDMAFSKKRVEDRKDWLRAFVPGTYVDYAVQDMPYTEFVNKELILFSMADNIRSIPSMVDGFKPSQRKVLFSCFKRNLKKEIKVAQLAGYISEHSAYHHGEVNLHGTIIGMAQNFVGSNNINLLSPNGQFGTRLMGGKDAASARYVFTNVESVTRLVFHPLDDPILNYLEDEGQSIEPQWYMPTIPMSLVNGSDGIGTGWSCGIPNYNPLDIIENLRRRIQAEPMKSMVPFYRGFKGEIVQKGGSD--NFLVQGRYEIVDESTIVISELPVKTWTQSYKQFLETLLDANT------IKDFKENHTDTAVLFTVVLSPESLAAIQQAPGGIMKKFKLESSLAISNMHLFDSNGQIKHYSSPLDIIEDFYGLRLEYYGYRKAAILKRLEHDIMILSNKMRFILAVIEGELVVNNRKKQDLLEELRMKKFDPMPRKSTAPTVAAAGEDDPVAEEDDDSHSEVTAKDYDYLLSLPMWSLTAERVQSLRDDLEEKNQERDTVFSTTLESMWLKDLHELELGLSKEE---------MKRLAQEESTMKQAGKAKSGKSQAKRKPPAKKRTHLDSDNDSGSDFELTKAKVKKQKDDDEKPKIEK---LAVVKPPVAVWKNPVEGPPAQTKVKKISKAPKKELN---ATASSDKPTKLFKLFEKKPAVSVAAESSEDE--------EVLSLSERLKRRAM---AEKNAESDED 1296