EWM24654.1, cds6015 (CDS) Nannochloropsis gaditana

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|585106176|gb|EWM24654.1| (dna ligase 1 [Nannochloropsis gaditana])

HSP 1 Score: 2064.66 bits (5348), Expect = 0.000e+0
Identity = 999/999 (100.00%), Postives = 999/999 (100.00%), Query Frame = 0

Query:    1 MNIGMAPSRKQQILPFAFESSTSNTSGSASSPTEKTGSIRNFFKRGPSKGLDFAARCKNQYEDARPKDSKEFNGETTGTVECGSEEMKVANCDMLAAHEITISNSAESERRSKKAEDKVVICAPTPAANIEVKDIQSKDIRATSTDNNDGINKKPKKRLRRLVDDEDEESDDDLRLINDNLQYARAALEVVAEHCGVKHSTSDGTTAGGIEHAKKMAATEGKMGGDDHNTSDGENEDSAEEGKVDKNGQNGSFGDENVDKEVRLNSNIVQPTLESSRPPVKPKKSSANKSKVIAGTTKDILEEIRGLIPGYSSLPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLNFGANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREEALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDYYNTSLECDVWFDPKIVWEVRAADLSLSPAHKGAIDKVEKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALVEATLSAGRSKFGQEEDDWCI 999
            MNIGMAPSRKQQILPFAFESSTSNTSGSASSPTEKTGSIRNFFKRGPSKGLDFAARCKNQYEDARPKDSKEFNGETTGTVECGSEEMKVANCDMLAAHEITISNSAESERRSKKAEDKVVICAPTPAANIEVKDIQSKDIRATSTDNNDGINKKPKKRLRRLVDDEDEESDDDLRLINDNLQYARAALEVVAEHCGVKHSTSDGTTAGGIEHAKKMAATEGKMGGDDHNTSDGENEDSAEEGKVDKNGQNGSFGDENVDKEVRLNSNIVQPTLESSRPPVKPKKSSANKSKVIAGTTKDILEEIRGLIPGYSSLPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLNFGANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREEALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDYYNTSLECDVWFDPKIVWEVRAADLSLSPAHKGAIDKVEKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALVEATLSAGRSKFGQEEDDWCI
Sbjct:    1 MNIGMAPSRKQQILPFAFESSTSNTSGSASSPTEKTGSIRNFFKRGPSKGLDFAARCKNQYEDARPKDSKEFNGETTGTVECGSEEMKVANCDMLAAHEITISNSAESERRSKKAEDKVVICAPTPAANIEVKDIQSKDIRATSTDNNDGINKKPKKRLRRLVDDEDEESDDDLRLINDNLQYARAALEVVAEHCGVKHSTSDGTTAGGIEHAKKMAATEGKMGGDDHNTSDGENEDSAEEGKVDKNGQNGSFGDENVDKEVRLNSNIVQPTLESSRPPVKPKKSSANKSKVIAGTTKDILEEIRGLIPGYSSLPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLNFGANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREEALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDYYNTSLECDVWFDPKIVWEVRAADLSLSPAHKGAIDKVEKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALVEATLSAGRSKFGQEEDDWCI 999          

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|1210528702|dbj|GAX12572.1| (DNA ligase 1 [Fistulifera solaris])

HSP 1 Score: 585.874 bits (1509), Expect = 0.000e+0
Identity = 330/691 (47.76%), Postives = 441/691 (63.82%), Query Frame = 0

Query:  313 SLPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLN--FGANPP--PLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREEALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDYYNTS--LECDVWFDPKIVWEVRAADLSLSPAHKGAIDKV-EKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALVEATLSAGRSKFGQEEDD 996
            +LPY  L   LA +E++T RL+I  ++ +L    +   P DL   LYL    + PA E  ELGIG+  ++ A+ +++G  ++ + Q  E A GDLGT AQ+ K+ Q TL   F A  P  PLT   VLQ+FR+IA   G +S   K+  I+ LL++C ++  E+K++VRGLQ KLR  LA+ TVL +LA +          LA +   R++++ ++  K +   P E        +V  L   +Q T  +  + E A  I+K+AYSE P+YD L+DA +  PLT + ++C L+PG+PV PMLAKP  S QEVL+R+ G  FT EYKYDGERAQ+HLL+DG+ K+FSRN  DT+ KYP++   ++ A Q   TS+V+DAE VA     GKL+PFQ+LSTRK+    AE  KVQ+++  FD++YLN R +L E L  RR LL+  F  IP KF +A  LDH E+GDT+ +E FLD A+ G CEGLMVKT++    YEP++RSLNWLKLKKDYL         G  DS+DLV LGA HG+GKR G YGA+LLACYD + EE+QSVCKIGTGFSEE L      L+  ++ + K   YN +  LECDVWFD   VWEV+AADLS S AH+GA+ K  E G+GI LRFPRF R R DK  EDAT+S+QI + Y  Q +V     AG S    ++DD
Sbjct:  186 TLPYAVLCQTLAEIEAITARLQIQQLLTKLFRSILLLAPHDLYTALYLTSNSVAPACENVELGIGDSLLIKAVAEAVG--SNRVKQLYEQA-GDLGTVAQQCKSKQTTLGGFFAAKTPKAPLTANLVLQTFREIAQTKGNQSQKWKVDKIKMLLVKC-QDPLEMKYIVRGLQGKLRIGLAQSTVLISLAHA----------LALTAYHRESENAENQTKTNY--PAE--------VVQYLD--KQQTIEK--RLEGAVQIIKRAYSEVPSYDALLDAALRVPLTAMYQACTLTPGIPVAPMLAKPTKSIQEVLQRLNGQKFTCEYKYDGERAQIHLLEDGTTKVFSRNLLDTSEKYPEVPVFVKAAQQSNVTSYVMDAEVVAFK--DGKLVPFQILSTRKKTEASAESAKVQIIVHAFDLMYLNGRSLLSESLETRRQLLQEHFLPIPEKFQYATYLDHKEDGDTTILEEFLDLAVKGCCEGLMVKTMDD--VYEPSRRSLNWLKLKKDYL--------EGLGDSVDLVPLGAYHGRGKRTGVYGAYLLACYDADTEEFQSVCKIGTGFSEEDLKGLADSLNQHVIPA-KTSQYNVTDQLECDVWFDAVQVWEVKAADLSKSSAHRGAVGKTGEAGRGIGLRFPRFERIREDKRPEDATTSDQILEMYYQQDVV-----AGVSNGDVDDDD 830          

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|1210513470|dbj|GAX27867.1| (DNA ligase 1 [Fistulifera solaris])

HSP 1 Score: 584.719 bits (1506), Expect = 0.000e+0
Identity = 325/676 (48.08%), Postives = 431/676 (63.76%), Query Frame = 0

Query:  313 SLPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLN--FGANPP--PLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREEALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDYYNTS--LECDVWFDPKIVWEVRAADLSLSPAHKGAIDKV-EKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALVEA 981
            +LPY  L   LA +E++T RL+I  ++ +L    +   P DL   LYL    + PA +  ELGIG+  ++ A+ +++G  ++ + Q  E A GDLGT AQ+ K+ Q TL   F A  P  PLT   VLQ+FR+IA   G +S   K+  I+ LL++C ++  E+K++VRGLQ KLR  LA+ TVL +LA +          LA +   R  D   + +K     P E           A  + +Q T  +  + + A  I+K+AYSE P+YD L+DA +  PLT + ++C L+PG+PV PMLAKP  S QEVL+R+ G  FT EYKYDGERAQ+HLL+DG+ K+FSRN  DT+ KYP++   ++ A Q   TS+V+DAE VA     GKL+PFQ+LSTRK+    AE  KVQ+++  FD++YLN R +L E L  RR LL+  F  IP KF +A  LDH E+GDT+ +E FLD+A+ G CEGLMVKT++    YEP++RSLNWLKLKKDYL         G  DS+DLV LGA HG+GKR G YGA+LLACYD + EE+QSVCKIGTGFSEE L      L+  I+ + K   YN +  LECDVWFD   VWEV+AADLS S AH+GA+ K  E G+GI LRFPRF R R DK  EDAT+S+QI + Y  Q +V A
Sbjct:  184 TLPYAVLCQTLAEIEAITARLQIQQLLTKLFRSILLLAPLDLYTALYLTSNSVAPACDNVELGIGDSLLIKAVAEAVG--SNRVKQLYEQA-GDLGTVAQQCKSKQTTLGGFFAAKTPKAPLTANLVLQTFREIAQTKGNQSQKWKVDKIKMLLVKC-QDPLEMKYIVRGLQGKLRIGLAQSTVLISLAHA----------LALTAYHR--DAENAENKTKSKYPAEV----------AQYLDKQQTIEK--RLDGAVQIIKRAYSEVPSYDALLDAALRVPLTAMYQACTLTPGIPVAPMLAKPTKSIQEVLQRLNGQKFTCEYKYDGERAQIHLLEDGTTKVFSRNLLDTSEKYPEVPVFVKAAQQSNVTSYVMDAEVVAFK--DGKLVPFQILSTRKKTESSAESAKVQIIVHAFDLMYLNGRSLLSESLETRRQLLQEHFFPIPEKFQYATYLDHKEDGDTTILEEFLDQAVKGCCEGLMVKTMDD--VYEPSRRSLNWLKLKKDYL--------EGLGDSVDLVPLGAYHGRGKRTGVYGAYLLACYDADTEEFQSVCKIGTGFSEEDLKNLADTLNQHIIPA-KTSQYNVTDQLECDVWFDAVQVWEVKAADLSKSSAHRGAVGKTGEAGRGIGLRFPRFERIREDKRPEDATTSDQILEMYYQQDVVAA 818          

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|219121358|ref|XP_002185904.1| (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >gi|209582753|gb|ACI65374.1| predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 583.563 bits (1503), Expect = 0.000e+0
Identity = 323/671 (48.14%), Postives = 425/671 (63.34%), Query Frame = 0

Query:  314 LPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLN--FG---ANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREEALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDYYNTS--LECDVWFDPKIVWEVRAADLSLSPAHKGAIDKV-EKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQ 976
            +PY  L      +E+VT RL I  I   LL +     P DL  +LYL    + PAYE  ELG+G+  +V A+ ++ G + + + ++ E   GDLG  A+  K+ QRTL   F    A   PLT   VL  FR+IA   G +S   K+  I+KL+++   E  E K++VRGLQ KLR  LA+ TVL  LA +   +P       + T +   D+           P E+    +D L         P+  R+   EAA AIVK+AYSE P+YD +++     PL  + R+C L+PG+PV PMLAKP  S QEVL+R+ GL FT EYKYDGERAQVHLL DG+ ++FSRN  DT+ KYP++   ++EA +    SFV+DAE VA +  +G+L+PFQ+LSTRK+  E AE  KVQV++  FD++YLN + +L++ L  RR+LL   F ++PGKF FA SLDH E+GDT+ +E FLD A+ G CEGLMVKTLE NA YEP++RSLNWLKLKKDYL         G  DS+DLV LGA HG+GKR G+YGA+LLACYD E EE+QSVCKIGTGFSEE L +    L+  ++ + K   Y+ S  LECDVWFD   VWE++AADLS S  H+GA  K  E+G+GI LRFPRF R R DK  E+AT+S+QI + Y  Q
Sbjct:    1 MPYSVLCQAFTDIEAVTSRLLIQQIFTTLLRQIAVQNPVDLYPVLYLASNDVAPAYECVELGVGDSILVRAIGEATGTNPAMVKKRYEEI-GDLGDVAKSFKSKQRTLGGFFQKSVAPKKPLTAAQVLHVFREIAQTKGNQSQKWKVENIKKLIVQ-ATEATETKYIVRGLQGKLRIGLAQSTVLIGLAHALALTPPITLCPHQPTLAELEDY-----------PAEARQYLDDTL---------PSEKRM---EAAVAIVKKAYSEVPSYDAIVNGAAKVPLHQLHRACTLTPGIPVAPMLAKPTKSIQEVLKRLSGLRFTCEYKYDGERAQVHLLPDGTTRVFSRNLLDTSEKYPEVPLFVKEACR-NVESFVLDAEVVAYNNKTGQLVPFQILSTRKKTEESAETAKVQVIVQAFDLMYLNGKSLLNQTLAARRSLLMEHFEAVPGKFQFAKSLDHKEDGDTTVLEEFLDSAVKGQCEGLMVKTLEVNAIYEPSRRSLNWLKLKKDYLD--------GLGDSVDLVPLGAYHGRGKRTGFYGAYLLACYDSETEEFQSVCKIGTGFSEEDLKSLAENLNQHVIPT-KSQQYSVSDVLECDVWFDAVQVWEIKAADLSKSSTHRGAEGKTGEEGRGIGLRFPRFERIRDDKRPEEATTSDQILEMYYQQ 636          

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|223995863|ref|XP_002287605.1| (ligase [Thalassiosira pseudonana CCMP1335] >gi|220976721|gb|EED95048.1| ligase [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0
Identity = 312/646 (48.30%), Postives = 425/646 (65.79%), Query Frame = 0

Query:  335 ITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLNFGANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVES-PREEALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTT-TSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDY-YNTSLECDVWFDPKIVWEVRAADLSLSPAHKGAIDKV-EKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQ 976
            +T++ R +L++  +   +DL  LLYL    + P+YE  ELG+G+  +V A+ ++ G +   + +K E  +GDLGT AQ +K  Q+TL  G  P  L  + VL+ FR+IA  SG +S   K+  I+ LL+R   +G E K+++RGLQ KLR  LA+ +VL +L+ + V + P++   L       +    +S+D ++       + +  DF    L     P      K E + AIVK+AYSE P++D L+DAL+SAPL ++ ++C L+PG+PV+PMLAKP  S  EVL+R+ G  FT EYKYDGERAQVH+  DG  K+FSR+  +T+ K+P++   ++EA + T+ TSFV+D E VA + V+ + +PFQVLSTRK+  E AED KV+V++  FD++YLN   +L  PL +RR L+R+ F  +PGKF FA SLDH E+GDT+ +E FLD A+ G CEGLMVKTL+ NA YEP++RSLNWLKLKKDYL         G  DS+DLV LGA HG+GKR G YGA+LLA Y+++ EE+QSVCKIGTGFSEE L      L   I+      Y  + SLECDVWFD   VWEV+AADLS S  HKGA+DK  E G+GI LRFPRF R R DK  EDATS++QI D Y +Q
Sbjct:    1 MTELFRMVLIRD-DALRSDLYPLLYLASNSVAPSYECVELGVGDSILVKAIGEASGTNPQMIKKKYE-KEGDLGTVAQTSKGKQKTLIGG--PKRLDVREVLKVFREIATTSGSQSQKWKVDKIKGLLVRA--KGSEAKYIIRGLQGKLRIGLAQSSVLASLSHAIVLTKPKDVVPLTE----ERLKEIRSIDADE-------KGAYPDF-ARKLCAENLPLD---VKLETSVAIVKKAYSEVPSFDSLLDALLSAPLQEMHKACNLTPGIPVEPMLAKPTKSVLEVLKRLNGARFTCEYKYDGERAQVHMTPDGKTKVFSRSLLNTSEKFPEVPLYVQEACKETSVTSFVLDTEVVAFNRVTQQFVPFQVLSTRKKTEESAEDAKVKVIVQAFDLMYLNGESLLDRPLAERRELMRKNFIPVPGKFQFATSLDHTEDGDTAVVEEFLDNAVKGQCEGLMVKTLDVNAAYEPSRRSLNWLKLKKDYL--------EGLGDSVDLVPLGAYHGRGKRTGVYGAYLLAVYEEDTEEFQSVCKIGTGFSEEDLKELAASLKPHIIPEKSSQYVVSDSLECDVWFDAVQVWEVKAADLSKSSTHKGAVDKTGESGRGIGLRFPRFERVRPDKKPEDATSADQILDMYYNQ 617          

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|353240611|emb|CCA72472.1| (probable CDC9-DNA ligase I [Serendipita indica DSM 11827])

HSP 1 Score: 553.518 bits (1425), Expect = 4.021e-180
Identity = 319/711 (44.87%), Postives = 422/711 (59.35%), Query Frame = 0

Query:  303 EIRGLIPGYSSLPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECC-------PTDLPVL--LYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLNFGANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRC-----GKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREEALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTS-PIEAFLDEAIAGNCEGLMVKTLEQNAT-YEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDYYNTSLECDVWFDPKIVWEVRAADLSLSPAHKGAIDKVEKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALVEATLSAGRSKFGQEEDDW 997
            + +G  P     PY  +T   + +E  T R+E T  +  LL   I          P    VL  +YL   ++ P Y   ELGIGE  ++ A+ +S G SA  +  + +  +GDLG  A  +KASQ+T+     P PLT   V    ++IA+ SG +S  +K  II KLL  C     G    E KF++R L+ KLR  LAE TV+ ALA + V   +E               NK+  KE +                              K E A  I+KQ YSE P+YD +I AL+   + D+   C+L+PG+P+KPMLAKP  +  EVL R +G  FT EYKYDGERAQVH L+DG+  +FSRNSED + KYPDL+  L   ++  T SFV DAEAVA+D  +GKL+PFQ LS RKRK+ K ED+ V+V LF FD+LYLN  P+LH+ L +RRALLR  F  +P +FDFA S D    GDT+  I+ FL+E++   CEGLMVK L+ + + YEP++RS+NWLKLKKDYL+        G  DSLDLVV+G  HG+GKR   YGAFLLACYD + EE+Q++CKIGTGFS+E L      L  L +S+ + D      + DVWF+PK+VWEV AADLSLSP +  A   VE+ +GI+LRFPRFIR R DK+++DAT  EQIA+ Y+ QAL +   S G+   G  +DD+
Sbjct:  176 DFKGAWPEGEPAPYALITQAFSLIEGTTKRIEKTSYLTSLLFTVISRSRQKDGREPDIDSVLQTVYLCINRLSPDYIGIELGIGESLLIKAISESTGRSAQVIKTELK-KEGDLGLVAMNSKASQKTI---FKPKPLTVPTVFARLKEIALTSGVQSQAKKTGIITKLLAACQTTIAGSTSSEAKFIIRSLEGKLRIGLAERTVIVALAHAAVLDEKER-------------RNKNWSKERLSS----------------------------KLEEATGILKQVYSEIPSYDLVIPALLKYGIWDLRAHCKLTPGIPLKPMLAKPTKAIGEVLDRFEGKKFTCEYKYDGERAQVHRLEDGTVAVFSRNSEDMSKKYPDLMESLPRCIKDNTKSFVFDAEAVAIDKNTGKLMPFQELSKRKRKDVKVEDIAVRVCLFAFDLLYLNGEPLLHKSLEERRALLREHFVPVPLEFDFAKSSD----GDTTEQIQTFLEESVKDGCEGLMVKMLQSDESFYEPSRRSVNWLKLKKDYLA--------GVGDSLDLVVVGGYHGKGKRTAVYGAFLLACYDADNEEWQTICKIGTGFSDEALQEHAKTLKELEISALRGDIKAGGAKPDVWFEPKVVWEVLAADLSLSPIYTAAQGLVEQ-RGISLRFPRFIRVRDDKSADDATGPEQIAEMYQRQALAQ---SKGKKGKGDADDDF 825          

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|950893539|ref|XP_014528222.1| (DNA ligase 1-like protein [Blastocystis sp. subtype 4] >gi|902862801|gb|KNB44747.1| DNA ligase 1-like protein [Blastocystis sp. subtype 4])

HSP 1 Score: 545.043 bits (1403), Expect = 4.791e-179
Identity = 304/691 (43.99%), Postives = 423/691 (61.22%), Query Frame = 0

Query:  313 SLPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGAD-GDLGTAAQRAKASQRTLNFGANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREEALLARSTKSRKTDHNKSLDKEDIHGPHESEPSR-NDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPT------TTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRK----------NEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLI----LSSGKPDYYN--TSLECDVWFDPKIVWEVRAADLSLSPAHKGAIDKVEKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALV 979
            ++PY+ L ++   VE ++ R ++  +        I   P DL   +YL+  ++ P ++  ELGIG+  I+ +L  + G   SS N K E    GDLG  A   K++Q+ +     PPPLT + + + F+ +A   G +S ++K  +I++L++     G+E+K+++RGLQ KLR  LAE +V+ ALA + V            T    T     LD        ++ P+R  D L+ A  +L                  KQ  SECP+YD +I AL+S+PL+D+   C L+PG+PV+PMLAKP     EVL R QGL FT EYKYDGERAQ+H L DG+ KIFSRNSED T KYPDL+  L + L  T        + ++D+E VA D  + ++LPFQVLSTR RK          + K EDVK+ VV+F+FD +YLN R +L EPL++RR +LR   + +PG+  FA ++   E  DT  +++FL E++A  CEGLMVKTL +NA+YEP+KRSLNWLKLKKDYL        +   DSLDLV +G  +G+GKR G +GAFLLACYD  +EEYQ+VCK+GTGFS+E L   +   +F      ++  KP +YN  + +E DVWF+P  VWEVRAADLS+SP H GAI  V+  KG+ALRFPRFIR R DK  E+ T+SEQ+A+ Y +Q++V
Sbjct:   21 AVPYKALAHLFTEVEKISERNKMITLTCNFFRSVIATTPADLLACVYLILNEVAPTFKGVELGIGDGIIIKSLQDATG--KSSANIKAELKKCGDLGDVAMTCKSTQKLI---FPPPPLTVRSLFKDFKSLAEMEGNKSQDRKSGVIKRLMV--AARGEEIKYIIRGLQGKLRIGLAEQSVICALAHAVV-----------LTPPSSTLPPPILD-----AAADTNPARLQDELIEATELL------------------KQVVSECPSYDAIIPALLSSPLSDLHNVCHLTPGIPVQPMLAKPTKGISEVLDRFQGLQFTCEYKYDGERAQIHKLADGTIKIFSRNSEDNTPKYPDLVATLHQVLSSTYCIPASVETCILDSECVAWDREAERILPFQVLSTRARKVSPLLIDQPQDVKEEDVKINVVIFMFDCIYLNGRSLLQEPLLERRKILRENIHEVPGRMQFATNM---ELDDTEQMQSFLTESVAHGCEGLMVKTLVENASYEPSKRSLNWLKLKKDYL--------KDLGDSLDLVPIGGYYGKGKRTGTFGAFLLACYDVNSEEYQTVCKLGTGFSDEDL---RKHSEFFQTNDNITEKKPLFYNVQSDVEPDVWFNPVQVWEVRAADLSISPVHTGAIGLVDPNKGVALRFPRFIRIREDKKPEECTNSEQVAEFYNNQSIV 656          

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|805772508|ref|XP_012136945.1| (PREDICTED: DNA ligase 1 isoform X2 [Megachile rotundata])

HSP 1 Score: 550.821 bits (1418), Expect = 1.517e-177
Identity = 289/676 (42.75%), Postives = 406/676 (60.06%), Query Frame = 0

Query:  314 LPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLNFGANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREE-ALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDY-YNTSLECDVWFDPKIVWEVRAADLSLSPAHKGAIDKVEKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALVEATLSAGR 987
            +PY  LT  L  +E  + RL+I +I+       I   P DL   +YL   ++GPAYE  ELG+ +  ++ A+ +  G + + +    +   GDLG  A+ ++++QRT+     P PLT  +V    R+IA  +G  SM++K+  I+ L + C     E ++L+R L  KLR  LAE +VL ALA +   +P E+   +  ++K   +D  K                                    QK+E    I+K  Y ECPNYD++I  L+   +  +   C+++PG+P+KPMLA P    QEVL R +GL FT E+KYDGERAQ+H  ++G   I+SRN E+ T+KYPD++   +     +  S ++D EAVA D  +  +LPFQVLSTRKRK+    D+KVQV +F+FD+LYLN  P++ +P ++RR LL+  F  + G++ FA SLD         ++ FLDE++ GNCEGLMVKTLEQ+ATYE  KRS NWLKLKKDYL         G  D+LD+VV+G   G+GKR G YG FLLACYDQE EEYQS+CKIGTGF+EE L      L   I+ + K  Y Y++SL+ D WF+P  VWE++ ADLSLSP HK A+  V+  KGI+LRFPRFIR R DK SEDATS++Q+A  Y SQ  ++  +++ +
Sbjct:  311 VPYIALTRTLELIEETSARLKIIEILSNYFRSVIVLRPDDLLPSVYLCLNQLGPAYEGIELGVADTTLMKAIAQCTGRTLAQIKADVQEV-GDLGIVAEGSRSNQRTM---FQPAPLTVPNVYSRLREIAQMTGSASMSKKLDKIQALFVAC--RLTEARYLIRSLAGKLRIGLAEQSVLQALALACAMTPPEQDPQILDASKKMSSDSFK------------------------------------QKYEKIALILKTTYCECPNYDKIIPVLLKEGVEALPSKCKITPGIPMKPMLAHPTKGVQEVLTRFEGLKFTCEWKYDGERAQIHFAENGDISIYSRNQENNTSKYPDIIGRFKNTKGESVKSCILDCEAVAWDNENKHILPFQVLSTRKRKDANEADIKVQVCVFMFDLLYLNGEPLVQQPFVKRRELLKENFKDVEGEWKFANSLD---TSSMEQVQDFLDESVKGNCEGLMVKTLEQDATYEIAKRSRNWLKLKKDYLD--------GVGDTLDVVVIGGYIGKGKRTGTYGGFLLACYDQENEEYQSICKIGTGFTEEDLQKHSEFLKEHIIPTNKSYYRYDSSLQPDHWFEPVQVWEIKCADLSLSPVHKAAVGIVDPEKGISLRFPRFIRIRDDKNSEDATSAQQVATMYNSQEQIKNQVTSSK 933          

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|805772514|ref|XP_012136947.1| (PREDICTED: DNA ligase 1 isoform X4 [Megachile rotundata])

HSP 1 Score: 549.666 bits (1415), Expect = 1.621e-177
Identity = 289/676 (42.75%), Postives = 406/676 (60.06%), Query Frame = 0

Query:  314 LPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLNFGANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREE-ALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDY-YNTSLECDVWFDPKIVWEVRAADLSLSPAHKGAIDKVEKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALVEATLSAGR 987
            +PY  LT  L  +E  + RL+I +I+       I   P DL   +YL   ++GPAYE  ELG+ +  ++ A+ +  G + + +    +   GDLG  A+ ++++QRT+     P PLT  +V    R+IA  +G  SM++K+  I+ L + C     E ++L+R L  KLR  LAE +VL ALA +   +P E+   +  ++K   +D  K                                    QK+E    I+K  Y ECPNYD++I  L+   +  +   C+++PG+P+KPMLA P    QEVL R +GL FT E+KYDGERAQ+H  ++G   I+SRN E+ T+KYPD++   +     +  S ++D EAVA D  +  +LPFQVLSTRKRK+    D+KVQV +F+FD+LYLN  P++ +P ++RR LL+  F  + G++ FA SLD         ++ FLDE++ GNCEGLMVKTLEQ+ATYE  KRS NWLKLKKDYL         G  D+LD+VV+G   G+GKR G YG FLLACYDQE EEYQS+CKIGTGF+EE L      L   I+ + K  Y Y++SL+ D WF+P  VWE++ ADLSLSP HK A+  V+  KGI+LRFPRFIR R DK SEDATS++Q+A  Y SQ  ++  +++ +
Sbjct:  281 VPYIALTRTLELIEETSARLKIIEILSNYFRSVIVLRPDDLLPSVYLCLNQLGPAYEGIELGVADTTLMKAIAQCTGRTLAQIKADVQEV-GDLGIVAEGSRSNQRTM---FQPAPLTVPNVYSRLREIAQMTGSASMSKKLDKIQALFVAC--RLTEARYLIRSLAGKLRIGLAEQSVLQALALACAMTPPEQDPQILDASKKMSSDSFK------------------------------------QKYEKIALILKTTYCECPNYDKIIPVLLKEGVEALPSKCKITPGIPMKPMLAHPTKGVQEVLTRFEGLKFTCEWKYDGERAQIHFAENGDISIYSRNQENNTSKYPDIIGRFKNTKGESVKSCILDCEAVAWDNENKHILPFQVLSTRKRKDANEADIKVQVCVFMFDLLYLNGEPLVQQPFVKRRELLKENFKDVEGEWKFANSLD---TSSMEQVQDFLDESVKGNCEGLMVKTLEQDATYEIAKRSRNWLKLKKDYLD--------GVGDTLDVVVIGGYIGKGKRTGTYGGFLLACYDQENEEYQSICKIGTGFTEEDLQKHSEFLKEHIIPTNKSYYRYDSSLQPDHWFEPVQVWEIKCADLSLSPVHKAAVGIVDPEKGISLRFPRFIRIRDDKNSEDATSAQQVATMYNSQEQIKNQVTSSK 903          

BLAST of EWM24654.1 vs. NCBI_GenBank
Match: gi|805772517|ref|XP_012136948.1| (PREDICTED: DNA ligase 1 isoform X5 [Megachile rotundata] >gi|805772520|ref|XP_012136949.1| PREDICTED: DNA ligase 1 isoform X5 [Megachile rotundata])

HSP 1 Score: 547.74 bits (1410), Expect = 3.654e-177
Identity = 289/676 (42.75%), Postives = 406/676 (60.06%), Query Frame = 0

Query:  314 LPYRTLTNVLAAVESVTGRLEITDIIRELLVKTIECCPTDLPVLLYLLCGKIGPAYEATELGIGEKNIVDALVKSLGCSASSLNQKGEGADGDLGTAAQRAKASQRTLNFGANPPPLTCQHVLQSFRDIAMCSGGRSMNQKISIIRKLLMRCGKEGDEVKFLVRGLQTKLRCRLAEPTVLTALAQSFVESPREE-ALLARSTKSRKTDHNKSLDKEDIHGPHESEPSRNDFLVHALAVLRQPTANRVQKWEAAEAIVKQAYSECPNYDRLIDALISAPLTDVLRSCRLSPGVPVKPMLAKPQDSFQEVLRRMQGLHFTMEYKYDGERAQVHLLQDGSFKIFSRNSEDTTTKYPDLLPILREALQPTTTSFVIDAEAVAVDPVSGKLLPFQVLSTRKRKNEKAEDVKVQVVLFVFDILYLNDRPILHEPLIQRRALLRRAFNSIPGKFDFAISLDHHENGDTSPIEAFLDEAIAGNCEGLMVKTLEQNATYEPTKRSLNWLKLKKDYLSSNAGPGGRGPADSLDLVVLGANHGQGKRAGWYGAFLLACYDQEAEEYQSVCKIGTGFSEEFLAAAKPQLDFLILSSGKPDY-YNTSLECDVWFDPKIVWEVRAADLSLSPAHKGAIDKVEKGKGIALRFPRFIRERADKASEDATSSEQIADAYRSQALVEATLSAGR 987
            +PY  LT  L  +E  + RL+I +I+       I   P DL   +YL   ++GPAYE  ELG+ +  ++ A+ +  G + + +    +   GDLG  A+ ++++QRT+     P PLT  +V    R+IA  +G  SM++K+  I+ L + C     E ++L+R L  KLR  LAE +VL ALA +   +P E+   +  ++K   +D  K                                    QK+E    I+K  Y ECPNYD++I  L+   +  +   C+++PG+P+KPMLA P    QEVL R +GL FT E+KYDGERAQ+H  ++G   I+SRN E+ T+KYPD++   +     +  S ++D EAVA D  +  +LPFQVLSTRKRK+    D+KVQV +F+FD+LYLN  P++ +P ++RR LL+  F  + G++ FA SLD         ++ FLDE++ GNCEGLMVKTLEQ+ATYE  KRS NWLKLKKDYL         G  D+LD+VV+G   G+GKR G YG FLLACYDQE EEYQS+CKIGTGF+EE L      L   I+ + K  Y Y++SL+ D WF+P  VWE++ ADLSLSP HK A+  V+  KGI+LRFPRFIR R DK SEDATS++Q+A  Y SQ  ++  +++ +
Sbjct:  255 VPYIALTRTLELIEETSARLKIIEILSNYFRSVIVLRPDDLLPSVYLCLNQLGPAYEGIELGVADTTLMKAIAQCTGRTLAQIKADVQEV-GDLGIVAEGSRSNQRTM---FQPAPLTVPNVYSRLREIAQMTGSASMSKKLDKIQALFVAC--RLTEARYLIRSLAGKLRIGLAEQSVLQALALACAMTPPEQDPQILDASKKMSSDSFK------------------------------------QKYEKIALILKTTYCECPNYDKIIPVLLKEGVEALPSKCKITPGIPMKPMLAHPTKGVQEVLTRFEGLKFTCEWKYDGERAQIHFAENGDISIYSRNQENNTSKYPDIIGRFKNTKGESVKSCILDCEAVAWDNENKHILPFQVLSTRKRKDANEADIKVQVCVFMFDLLYLNGEPLVQQPFVKRRELLKENFKDVEGEWKFANSLD---TSSMEQVQDFLDESVKGNCEGLMVKTLEQDATYEIAKRSRNWLKLKKDYLD--------GVGDTLDVVVIGGYIGKGKRTGTYGGFLLACYDQENEEYQSICKIGTGFTEEDLQKHSEFLKEHIIPTNKSYYRYDSSLQPDHWFEPVQVWEIKCADLSLSPVHKAAVGIVDPEKGISLRFPRFIRIRDDKNSEDATSAQQVATMYNSQEQIKNQVTSSK 877