EWM30097.1, cds581 (CDS) Nannochloropsis gaditana

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|585112739|gb|EWM30097.1| (sphingolipid delta 4 desaturase c-4 hydroxylase protein des2 [Nannochloropsis gaditana])

HSP 1 Score: 744.577 bits (1921), Expect = 0.000e+0
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0

Query:    1 MDIGSLCLCDGLGGPWRSLSLLRPPLPILPPRTQAFLLPSHWSHAPNPRHHPRLRFPFSHVTTAQVLFPGIMCPGPQDGKGTGLVENPLVAAAAGLYAETEQPDKDEGVWDFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLGNNHFDPFLVPFQTDLVFLPPSCSRPDLRSLFPVPDDTDMAVAFLRA 365
            MDIGSLCLCDGLGGPWRSLSLLRPPLPILPPRTQAFLLPSHWSHAPNPRHHPRLRFPFSHVTTAQVLFPGIMCPGPQDGKGTGLVENPLVAAAAGLYAETEQPDKDEGVWDFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLGNNHFDPFLVPFQTDLVFLPPSCSRPDLRSLFPVPDDTDMAVAFLRA
Sbjct:    1 MDIGSLCLCDGLGGPWRSLSLLRPPLPILPPRTQAFLLPSHWSHAPNPRHHPRLRFPFSHVTTAQVLFPGIMCPGPQDGKGTGLVENPLVAAAAGLYAETEQPDKDEGVWDFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLGNNHFDPFLVPFQTDLVFLPPSCSRPDLRSLFPVPDDTDMAVAFLRA 365          

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|553192724|ref|XP_005855632.1| (sphingolipid delta-4 desaturase [Nannochloropsis gaditana CCMP526] >gi|422293421|gb|EKU20721.1| sphingolipid delta-4 desaturase [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 406.371 bits (1043), Expect = 1.307e-138
Identity = 209/248 (84.27%), Postives = 210/248 (84.68%), Query Frame = 0

Query:   72 MCPGPQDGKGTGLVENPLVAAAAGLYAETEQPDKDEGVWDFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG 319
            MCPGPQDGKGTGLVENPLVAAAAGLYAETEQPDKDEGVWDFFRSYTDEPHKSR                  PW+                    APFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG
Sbjct:    1 MCPGPQDGKGTGLVENPLVAAAAGLYAETEQPDKDEGVWDFFRSYTDEPHKSRSWP---------------PWS-----------------FATAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG 216          

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|1210517609|dbj|GAX23524.1| (sphingolipid Delta-4 desaturase [Fistulifera solaris])

HSP 1 Score: 236.113 bits (601), Expect = 1.551e-71
Identity = 111/218 (50.92%), Postives = 149/218 (68.35%), Query Frame = 0

Query:  102 QPDKDEGVWDFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG 319
            QP KD    DFF SYT+EPH +RR+ IL  +P++++LFGH P  KY    ++S Q+  A  +R     + +   Y + G+CNH L L MHE++HNL FK++  NR+FS+  NLP+GIP+AISFKRYH+EHHKYQG+  VDVD+PT  EG++F+   +K LFV     FY+LRPLFV PKKP  WE +N    +TFDA I Y LG  ++FYL++ T LG
Sbjct:   24 QPGKD----DFFWSYTEEPHATRRKQILAKYPQVKELFGHDPNFKYVVTLLVSIQIGMAYYMRFCSAWVFVLGAYAVGGTCNHMLMLAMHELSHNLGFKKMIHNRIFSLFANLPIGIPSAISFKRYHLEHHKYQGEVGVDVDIPTPAEGRIFQSTFMKFLFVMNQIFFYALRPLFVNPKKPGKWEFINAACCITFDAAILYTLGPWAMFYLILSTYLG 237          

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|223995251|ref|XP_002287309.1| (acyl desaturase/hydroxylase, partial [Thalassiosira pseudonana CCMP1335] >gi|220976425|gb|EED94752.1| acyl desaturase/hydroxylase, partial [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 230.72 bits (587), Expect = 6.618e-70
Identity = 113/225 (50.22%), Postives = 152/225 (67.56%), Query Frame = 0

Query:   95 GLYAETEQPDKDEGVWDFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG 319
            G   + E+ +K +    F  S+ +EPH+ R++ IL  HP+I++  G  P TKYK A  ++ Q+++  +L+ AP+   LF  YTLSGS NH +TL MHE++HNL  K +  NR  +I+ NLP+GIPAA SFKRYHMEHHK+QG++ VDVD+PTD EG  F   P K+L+  L P FYSLRPL V PK+P+ WE +N+L  + FDA I Y  G + L YLVVGTL G
Sbjct:    2 GEILDREREEKVQAEPQFRWSHDEEPHRRRKQEILSHHPKIKQFMGIDPTTKYKVAFWVAFQLLSLHLLQGAPWYTWLFCCYTLSGSINHMMTLAMHELSHNLGAKGVVPNRFLAILANLPMGIPAAASFKRYHMEHHKFQGEDVVDVDIPTDFEGWFFTSTPRKILWCLLQPLFYSLRPLAVNPKQPTRWEFINILCIIVFDAAIVYFWGIRELLYLVVGTLFG 226          

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|1210530822|dbj|GAX10773.1| (sphingolipid Delta-4 desaturase [Fistulifera solaris])

HSP 1 Score: 231.491 bits (589), Expect = 8.184e-70
Identity = 109/218 (50.00%), Postives = 148/218 (67.89%), Query Frame = 0

Query:  102 QPDKDEGVWDFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG 319
            QP KD    DFF SYT+EPH +RR+ IL  +P++++LFGH P  KY    ++S Q+  A  +R     + +   Y + G+CNH L L MHE++HNL FK++  NR+FS+  NLP+GIP+AISFKRYH+EHHKYQG+  VDVD+PT  EG++F+   +K LFV     FY+LRPLFV PKKP  WE +N +  + FDA I   LG  ++FYL++ T LG
Sbjct:   24 QPGKD----DFFWSYTEEPHATRRKEILAKYPQVKELFGHDPNFKYVVTLLVSIQIGMAYYMRFCSGWVFVLGAYAVGGTCNHMLMLAMHELSHNLGFKKMIHNRIFSLFANLPIGIPSAISFKRYHLEHHKYQGEVGVDVDIPTPAEGRIFQSTFMKFLFVMNQIFFYALRPLFVNPKKPGKWEFINAVCCIAFDAAILCTLGPWAMFYLILSTYLG 237          

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|676451698|ref|XP_009052791.1| (hypothetical protein LOTGIDRAFT_150030 [Lottia gigantea] >gi|556107774|gb|ESO96426.1| hypothetical protein LOTGIDRAFT_150030 [Lottia gigantea])

HSP 1 Score: 221.476 bits (563), Expect = 4.508e-66
Identity = 102/212 (48.11%), Postives = 147/212 (69.34%), Query Frame = 0

Query:  111 DFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAF--KRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLGN 320
            DF  SYTDEPH +RR+ IL  +P+++KL G  P  KY    ++  Q +A  ++R A +P+LL  TY   G+ NH L L +HE++HNLA+   +   NR+   I NLP+G+P +ISFK+YH+EHH+YQGD   DVD+P+++EGK+F    +K+++V L P FY++RP FV PK  S  E++N++ Q++FD L+ Y  GWKSLFYL+ GTLLG 
Sbjct:    9 DFEWSYTDEPHATRRKEILGKYPQMKKLMGSDPNLKYSVLLLVVIQCLALYLVRDASWPMLLVATYCFGGTVNHSLNLAIHEISHNLAYGHSKPLWNRLLGFIANLPIGVPTSISFKKYHLEHHRYQGDVLRDVDVPSEIEGKIFVNTFMKVIWVILQPLFYAIRPFFVYPKPLSMLEVINIIIQVSFDTLVVYTCGWKSLFYLIAGTLLGT 220          

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|698815158|ref|XP_009840608.1| (hypothetical protein H257_14482 [Aphanomyces astaci] >gi|574104338|gb|ETV69870.1| hypothetical protein H257_14482 [Aphanomyces astaci])

HSP 1 Score: 221.09 bits (562), Expect = 7.786e-66
Identity = 99/209 (47.37%), Postives = 139/209 (66.51%), Query Frame = 0

Query:  111 DFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG 319
            DF+   TDEPH +RR+ I   +P+I+ LFGHCP TKY     ++ Q + A V +   + + L + Y + G+ NH + + MHE++HNL FK+   NR+ S+I N+P+G+PAAISFKRYHMEHH+YQG++ VDVD+PT +EGK+F     KL+FVF    FYSLRPLFV PK P  WE VN  + + ++  I++  G   L Y +  T+LG
Sbjct:   19 DFYWEKTDEPHATRRKLITAKYPQIKSLFGHCPRTKYYVVVAVTVQTLMAYVTKDKSWAVWLAVAYIVGGTFNHMMMMAMHELSHNLGFKKPVYNRILSLIANMPMGVPAAISFKRYHMEHHRYQGEDGVDVDIPTVLEGKIFNNKITKLIFVFTQAFFYSLRPLFVNPKTPGFWEFVNYASCIAYNYAIYHFCGASGLGYCLASTVLG 227          

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|695411733|ref|XP_009525556.1| (fatty acid desaturase family, Zn-binding site [Phytophthora sojae] >gi|348676681|gb|EGZ16498.1| fatty acid desaturase family, Zn-binding site [Phytophthora sojae])

HSP 1 Score: 220.32 bits (560), Expect = 2.073e-65
Identity = 102/209 (48.80%), Postives = 142/209 (67.94%), Query Frame = 0

Query:  111 DFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG 319
            DF  + TDEPH +RR+ I+  +PE++KLFGHC  TKY   A ++ Q   A+  ++  +P+ +FL Y + G+ NH + L MHEV+HNLAFK+ F N++  I  NLP+G+P+++SFKRYH+EHH+YQG++ VDVDLPT+ EGK+F     KLLFV     FY  RPL V PK P  WE  N  A L+++  I+   G+  L YL+VGT+LG
Sbjct:   34 DFHWTPTDEPHATRRKLIMAKYPEVKKLFGHCWKTKYIITATVALQTYLALTAQYMSWPVYMFLMYVVGGTANHAMMLGMHEVSHNLAFKKPFHNKLLGIFANLPVGVPSSVSFKRYHLEHHRYQGEDGVDVDLPTEFEGKIFTNKSTKLLFVIFQVFFYGGRPLIVNPKVPGVWEFFNATACLSYNYAIYVFGGFSGLLYLLVGTMLG 242          

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|813149925|ref|XP_012200609.1| (hypothetical protein SPRG_06402 [Saprolegnia parasitica CBS 223.65] >gi|641534604|gb|KDO28543.1| hypothetical protein SPRG_06402 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 216.468 bits (550), Expect = 4.502e-64
Identity = 101/209 (48.33%), Postives = 132/209 (63.16%), Query Frame = 0

Query:  111 DFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAFKRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG 319
            DF     DEPH +RR+ I   +P+I+ LFG CP TKY+ A  +S QV     +    +   +   Y + G+ NH + L MHE+AHNL FK++  NR+ S++ N PLG+PAAISFKRYHMEHH+YQG++ VDVD+PT  EGK F     K LFV     FYSLRPL V PK P  WE +N  + + F++ I+Y  GW  L YL + TLLG
Sbjct:   18 DFTWDTNDEPHATRRKLISAKYPQIKSLFGPCPRTKYQVAVAVSLQVAMTFYVHDKSWATYVAAAYVIGGTINHMMMLAMHELAHNLGFKKMLYNRLLSLLANAPLGVPAAISFKRYHMEHHRYQGEDGVDVDIPTVAEGKFFTTRFRKFLFVCCQSLFYSLRPLLVNPKAPGLWEFINYASCIAFNSFIYYFCGWSGLCYLFLSTLLG 226          

BLAST of EWM30097.1 vs. NCBI_GenBank
Match: gi|1036678940|ref|XP_016998725.1| (PREDICTED: sphingolipid delta(4)-desaturase DES1 [Drosophila takahashii])

HSP 1 Score: 215.312 bits (547), Expect = 7.019e-64
Identity = 105/211 (49.76%), Postives = 144/211 (68.25%), Query Frame = 0

Query:  111 DFFRSYTDEPHKSRREAILKAHPEIRKLFGHCPWTKYKAAAIISTQVMAAVVLRHAPFPLLLFLTYTLSGSCNHFLTLIMHEVAHNLAF--KRLFANRVFSIIVNLPLGIPAAISFKRYHMEHHKYQGDETVDVDLPTDVEGKLFRGGPLKLLFVFLMPAFYSLRPLFVRPKKPSNWELVNVLAQLTFDALIFYILGWKSLFYLVVGTLLG 319
            DF   YT+EPH SRR+ IL+ +P+I+KLFGH P  K+ A A++ TQ++A  V++  P+  LL   Y   G  NH L L +HE++HNLAF   R   NR+   I NLP+G+P +ISFK+YH+EHH+YQGDE +D D+PT +E +LF     K L+V L P FY  RPL + PK P+  E++N + QLTF+ALI Y LGWK L YL++G++L 
Sbjct:    9 DFEWVYTEEPHASRRKLILEKYPQIKKLFGHDPNFKWVAGAMVLTQILALFVVKDLPWSWLLVAAYCFGGIINHSLMLAVHEISHNLAFGHSRPLHNRILGFICNLPIGLPMSISFKKYHLEHHRYQGDEAIDTDIPTLLEARLFDTTFGKFLWVCLQPFFYIFRPLVINPKPPTRLEIINTVVQLTFNALIVYFLGWKPLAYLLIGSILA 219