EWM25228.1, cds5441 (CDS) Nannochloropsis gaditana

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|585106920|gb|EWM25228.1| (diacylglycerol kinase [Nannochloropsis gaditana])

HSP 1 Score: 1230.31 bits (3182), Expect = 0.000e+0
Identity = 603/603 (100.00%), Postives = 603/603 (100.00%), Query Frame = 0

Query:    1 MKLIQYFGTALCVVILSCVTNIIPGGRIALGRPFSRLFGGSSRNLRAEVEAAVPHFIVPEDRVEYPTPKLAALKSKLKEIGHHKAMGHPHQHQGLDGRRRVSLHPSHRPAPSSLGAAEDKEQEEEGGEEEEEGQEGVIAPPAWKPGHMNPRDSSSDMGKATKGKPGTPSAFLPLGVPPPSLFPPSARPIRRSPWSLLFRRGLPRPRRKRPIGINRIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVSKELLQYNEWLVGQGKLDAAGKDQLLQAFKRRLQVSQ 603
            MKLIQYFGTALCVVILSCVTNIIPGGRIALGRPFSRLFGGSSRNLRAEVEAAVPHFIVPEDRVEYPTPKLAALKSKLKEIGHHKAMGHPHQHQGLDGRRRVSLHPSHRPAPSSLGAAEDKEQEEEGGEEEEEGQEGVIAPPAWKPGHMNPRDSSSDMGKATKGKPGTPSAFLPLGVPPPSLFPPSARPIRRSPWSLLFRRGLPRPRRKRPIGINRIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVSKELLQYNEWLVGQGKLDAAGKDQLLQAFKRRLQVSQ
Sbjct:    1 MKLIQYFGTALCVVILSCVTNIIPGGRIALGRPFSRLFGGSSRNLRAEVEAAVPHFIVPEDRVEYPTPKLAALKSKLKEIGHHKAMGHPHQHQGLDGRRRVSLHPSHRPAPSSLGAAEDKEQEEEGGEEEEEGQEGVIAPPAWKPGHMNPRDSSSDMGKATKGKPGTPSAFLPLGVPPPSLFPPSARPIRRSPWSLLFRRGLPRPRRKRPIGINRIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVSKELLQYNEWLVGQGKLDAAGKDQLLQAFKRRLQVSQ 603          

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|585106921|gb|EWM25229.1| (diacylglycerol kinase [Nannochloropsis gaditana])

HSP 1 Score: 996.882 bits (2576), Expect = 0.000e+0
Identity = 486/486 (100.00%), Postives = 486/486 (100.00%), Query Frame = 0

Query:    1 MKLIQYFGTALCVVILSCVTNIIPGGRIALGRPFSRLFGGSSRNLRAEVEAAVPHFIVPEDRVEYPTPKLAALKSKLKEIGHHKAMGHPHQHQGLDGRRRVSLHPSHRPAPSSLGAAEDKEQEEEGGEEEEEGQEGVIAPPAWKPGHMNPRDSSSDMGKATKGKPGTPSAFLPLGVPPPSLFPPSARPIRRSPWSLLFRRGLPRPRRKRPIGINRIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEG 486
            MKLIQYFGTALCVVILSCVTNIIPGGRIALGRPFSRLFGGSSRNLRAEVEAAVPHFIVPEDRVEYPTPKLAALKSKLKEIGHHKAMGHPHQHQGLDGRRRVSLHPSHRPAPSSLGAAEDKEQEEEGGEEEEEGQEGVIAPPAWKPGHMNPRDSSSDMGKATKGKPGTPSAFLPLGVPPPSLFPPSARPIRRSPWSLLFRRGLPRPRRKRPIGINRIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEG
Sbjct:    1 MKLIQYFGTALCVVILSCVTNIIPGGRIALGRPFSRLFGGSSRNLRAEVEAAVPHFIVPEDRVEYPTPKLAALKSKLKEIGHHKAMGHPHQHQGLDGRRRVSLHPSHRPAPSSLGAAEDKEQEEEGGEEEEEGQEGVIAPPAWKPGHMNPRDSSSDMGKATKGKPGTPSAFLPLGVPPPSLFPPSARPIRRSPWSLLFRRGLPRPRRKRPIGINRIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEG 486          

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|303277079|ref|XP_003057833.1| (predicted protein [Micromonas pusilla CCMP1545] >gi|226460490|gb|EEH57784.1| predicted protein [Micromonas pusilla CCMP1545])

HSP 1 Score: 239.195 bits (609), Expect = 7.323e-69
Identity = 144/399 (36.09%), Postives = 212/399 (53.13%), Query Frame = 0

Query:  219 LPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSS--------DRHRQPVKYML--GYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVS-------KELLQYNEWLVGQGKLDAAGKDQLLQAFKRRLQ 600
            LP   +PL+  VNS+SGG+ G  L  +L+  L+  QV D+ K D K AL L+ +LP    ++VCGGDGT + + + ++ +      P + I+PLGTGND+ARVL W       GGY+   ND     L+ +      A    +DRWE+ +  A P           RHR   + ++   YLGIGVD + AL FH+ R+  P+LF S T NK  Y L G RDF   SC  +   V +  DG    LPP+TE  I+LNINS AGGV +W     GG   G    S   DG ++VV + G LHLG++  G+D+P+R+ QA++V++  +   P  VDGEPW  P C + ++   +A +L+  +       K++    EW      +    ++ ++    RR +
Sbjct:   25 LPDDASPLLVFVNSRSGGQLGGYLTSQLKQNLNPLQVVDLHKTDPKFALRLFSNLP-KLRIMVCGGDGTVAWILQALEELVEIDPKPPVGILPLGTGNDLARVLGW------GGGYY---NDLVSELLVQI----QEAHPAVLDRWEVGIIPADPEGPPPSPKKRRRHRPGTETLVFQNYLGIGVDAQAALRFHRTRNIRPHLFFSATTNKILYGLFGARDFVEHSCAGMNQHVHVIADGVRRDLPPETEGIILLNINSFAGGVRMWE----GGDGHGN---SSMQDGMVDVVVVFGALHLGQLNWGVDKPVRICQARDVKVIVEKGFPMHVDGEPWEQPACTIDIKLRNKALMLRRTADASGMTVKKMADTLEWARKHEIISNDQREMIMNEAYRRAE 402          

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|1205977070|ref|XP_021302039.1| (diacylglycerol kinase 2 [Sorghum bicolor] >gi|992160458|gb|KXG23275.1| hypothetical protein SORBI_3008G077600 [Sorghum bicolor])

HSP 1 Score: 245.743 bits (626), Expect = 8.322e-69
Identity = 156/425 (36.71%), Postives = 224/425 (52.71%), Query Frame = 0

Query:  186 ARPIRRSPWSLLFRRGLPRPRRKRPIGINRIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDI---QKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVSKELLQY-----NEWLVG---QGKLDAAGKDQLLQAFKRRL 599
            A+P+ ++     +R G+P  R ++      +  LP    PL+  +N KSGGR G +L +RL   L+  Q+F++   Q  D+   L L+ H      +LVCGGDGT + V + ++   ++  PP +AI+PLGTGND++RV+ W  G          S+   +  + ++ +D+  A    +DRW + + E   +  +  + VK+M  YLGIG D KVA DFH  R+  P  F S  +NK  YA  G +D   RSC +LP  V L  DG+ I +P   E  IV+NI S+ GGV+LW         +  F      D  LEVV ISG  HLG+++VGL R  RLAQ K +R+   S  P QVDGEPW  P   L + H GQ  +L+  S+E   +     +E LV     G +DA+ K  LL     RL
Sbjct:  293 AKPLSQNSLKQTYRSGIPNERVRK----YELVDLPQDSRPLLVFINGKSGGRNGPSLRRRLNMLLNPIQIFELSAPQGPDV--GLQLF-HNVKHFRILVCGGDGTVAWVLDAIEKQNYE-SPPPVAILPLGTGNDLSRVMRWGGG---------LSSVEREGGIYALLNDVDHAAVTVLDRWNVAIKEKNGTEGQCTKQVKFMTNYLGIGCDAKVAYDFHTSREEKPDKFCSQFVNKLIYAREGAKDMMDRSCSDLPWHVSLEVDGENIEIPEDAEGVIVMNIPSYMGGVDLWQN---DNDHDDDFSLQSIHDKILEVVCISGTWHLGKLQVGLSRAHRLAQGKVIRLHLHSSFPVQVDGEPWIQPPGCLEISHRGQMFMLRRTSEEPTGHAAAIMSEVLVNAECNGVIDASQKRLLLHEIALRL 697          

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|255072845|ref|XP_002500097.1| (predicted protein [Micromonas commoda] >gi|226515359|gb|ACO61355.1| predicted protein [Micromonas commoda])

HSP 1 Score: 237.654 bits (605), Expect = 2.342e-68
Identity = 143/372 (38.44%), Postives = 197/372 (52.96%), Query Frame = 0

Query:  198 FRRGLPRPRRKRPIGINRI--KTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVS 567
             R+ L + R     GI  I    LP    PL+  VNS+SGG+ G  LF +L   L+  QV D+ K D K AL  +C LP    +LVCGGDGT + + + ++ +E     P + I+PLGTGND+ARVL W       GG F  +ND     L+ +      A    +DRWE+ +T   P +       K    YLGIGVD + AL FH+ R+  P LF S   NK  Y + G RDF   SC  +   V L  DG    LPP+TE  I+LNINS AGGV +W         EG +  S   DG ++VV + G LHLG++  G+D+P+R+ QA+ V++  +   P  VDGEPW    C + +    +A +L+  +
Sbjct:    1 MRKILRKKRNGEYTGITSIDPSLLPEDSIPLLVFVNSRSGGQLGGYLFNQLGKNLNPLQVVDLYKTDPKVALRQFCDLPR-VRVLVCGGDGTVAWILQALEALEEIDPKPPVGILPLGTGNDLARVLGW-------GGGF--ANDLISELLMQI----QEAHPAVLDRWEVNITPQDPGAPPPSPKKKPKENYLGIGVDAQAALRFHRTRNVRPQLFFSAFTNKLLYGIFGARDFVEHSCAGMHQHVHLIADGVRRELPPETEGIILLNINSFAGGVRMWESS------EG-YGASSMQDGMVDVVVVFGALHLGQLNWGVDKPVRICQARHVKVIVERGYPMHVDGEPWEQAACTIDIGLRNKALMLRRTA 351          

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|674949022|emb|CDX84441.1| (BnaC03g50590D [Brassica napus])

HSP 1 Score: 238.039 bits (606), Expect = 4.022e-68
Identity = 152/413 (36.80%), Postives = 216/413 (52.30%), Query Frame = 0

Query:  201 GLPRPRRKRPIGINRIKT-----LPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPW-RLPRCELTLRHNGQAPVLQHVSKE-----LLQYNEWLVG---QGKLDAAGKDQLLQAFKRRL 599
            G+  P+R +   IN +K      LPP   PL+  +N+KSGG+ G  L +RL   L+  QVF++      +A    C       +LVCGGDGT + V + ++   ++  PP +AI+PLGTGND++RVL W  G     G           +L +   D+  A    +DRW +++ E        R+  K+M+ YLGIG D KVA +FH +R   P  F S  +NK  YA  G RD   R+C +LP +V L  DGK I +P  +E  IVLNI S+ GGV+LW         +  F      D  LEVV + G  HLG+++VGL +  RLAQ K ++I   S  P Q+DGEP+ + P C L + H+GQ  +L+  S E         NE L+    +G ++A  K +LLQ     L
Sbjct:   59 GIGAPKRSKNAVINCMKKYSLVDLPPDARPLLVFINAKSGGQLGPFLHRRLNMLLNPVQVFELGSCQGPDAGLDLCSKVKYFRVLVCGGDGTVAWVLDAIEKRNFE-SPPPVAILPLGTGNDLSRVLQWGRGISVVDG---------QGSLRTFLQDIDHAAVTMLDRWSVKIVEESTKIFPARESHKFMMNYLGIGCDAKVAYEFHMMRQENPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWLEVDGKDIDIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDD-DDNFSIQSMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIKIHVSSPFPVQIDGEPFIQQPGC-LEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINACQKKELLQQMALHL 459          

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|293335249|ref|NP_001169174.1| (uncharacterized protein LOC100383024 [Zea mays] >gi|223975311|gb|ACN31843.1| unknown [Zea mays] >gi|1142645500|gb|AQK40486.1| Diacylglycerol kinase 2 [Zea mays])

HSP 1 Score: 243.817 bits (621), Expect = 5.053e-68
Identity = 154/423 (36.41%), Postives = 218/423 (51.54%), Query Frame = 0

Query:  186 ARPIRRSPWSLLFRRGLPRPRRKRPIGINRIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEA-LSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVSKELLQY-----NEWLVG---QGKLDAAGKDQLLQAFKRRL 599
            A+P+ +      +R  +P  R ++      +  LP    PL+  +N KSGGR G +L +RL   L+  Q+F++      E  L L+ H      +LVCGGDGT + V + ++   ++  PP +AI+PLGTGND++RV+ W  G          S+      + ++ +D+  A    +DRW + + E   +     + +K+M  YLGIG D KVA DFH  R+  P  F S  +NK  YA  G +D   RSC +LP  V L  DGK I +P   E  IV+NI S+ GGV+LW            F+     D  LEVV ISG  HLG+++VGL R  RLAQ K +R+   S  P QVDGEPW  P   L + H GQ  +L+  S+E   +     +E LV     G +DA+ K  LL     RL
Sbjct:  291 AKPLSQHSVKQTYRSVIPNGRVRK----YELVDLPQDSRPLLVFINGKSGGRNGPSLRRRLNMLLNPIQIFELSASQAPEVGLQLF-HNVKHFRILVCGGDGTVAWVLDAIEKQNYE-SPPPVAILPLGTGNDLSRVMRWGGG---------LSSVERQGGIYALLNDVDHAAVTVLDRWNVTIKEKNGTEGECTKQIKFMTNYLGIGCDAKVAYDFHTTREEKPDKFCSQFVNKLIYAREGAKDMMDRSCSDLPWHVSLEVDGKNIEIPEDAEGVIVMNIPSYMGGVDLWQN---DNDHNDDFRLQSIHDKILEVVCISGTWHLGKLQVGLSRAHRLAQGKVIRLHLHSSFPVQVDGEPWIQPPGCLEISHRGQMFMLRRTSEEPTGHAAAIMSEVLVNAECNGVIDASQKRLLLHEIALRL 695          

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|573963553|ref|XP_006663912.1| (PREDICTED: diacylglycerol kinase 2 [Oryza brachyantha])

HSP 1 Score: 243.432 bits (620), Expect = 6.786e-68
Identity = 152/393 (38.68%), Postives = 209/393 (53.18%), Query Frame = 0

Query:  216 IKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEA-LSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVSKELLQY-----NEWLVG---QGKLDAAGKDQLLQAFKRRL 599
            I  LP    PL+  +N KSGGR G +L +RL   L+  Q+F++      E  L L+ H      +LVCGGDGT + V + ++   ++  PP +AI+PLGTGND++RV+ W  G          S+      + ++ +D+  A    +DRW + + E   + D+  + VK+M  Y+G+G D KVA DFH  R+  P  F S  +NK  YA  G +D   RSC +LP  V L  DGK I +P   E  IVLNI S+ GGV+LW         +  F      D  LEVV ISG+ HLG+++VGL R  RLAQ K +R+   S  P QVDGEPW  P   L + H GQ  +L+  S+E   +     +E LV     G +DAA K  LL     RL
Sbjct:  327 IVDLPQDSRPLLVFINGKSGGRSGPSLRRRLNMLLNPIQIFELSASQGPEVGLQLF-HNVKHFRILVCGGDGTVAWVLDAIEKQNYE-SPPPVAILPLGTGNDLSRVMRWGGG---------LSSVEGQGGICALLNDVDHAAVTVLDRWNVAIKEKNGAEDQCTKQVKFMTNYIGVGCDAKVAYDFHTTREERPDKFCSQFVNKLIYAREGAKDIMDRSCSDLPWHVSLEVDGKNIEIPEDAEGVIVLNIPSYMGGVDLWQN---DNEHDDDFGLQSVHDKMLEVVCISGMWHLGKLQVGLSRAHRLAQGKVIRLHLHSSFPVQVDGEPWIQPPGCLEISHRGQMFMLRRTSEEPTGHAAAIMSEVLVNAECNGVIDAAQKRLLLHEIALRL 705          

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|1002314756|ref|XP_015620748.1| (PREDICTED: diacylglycerol kinase 2 [Oryza sativa Japonica Group] >gi|108862351|gb|ABA96787.2| Diacylglycerol kinase 1, putative, expressed [Oryza sativa Japonica Group] >gi|222616828|gb|EEE52960.1| hypothetical protein OsJ_35604 [Oryza sativa Japonica Group])

HSP 1 Score: 243.432 bits (620), Expect = 6.856e-68
Identity = 145/393 (36.90%), Postives = 205/393 (52.16%), Query Frame = 0

Query:  215 RIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDLKEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWPEYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVSKELLQYNEWLVGQ--------GKLDAAGKDQLLQAFKRRL 599
             I  LP    PL+  +N KSGGR G +L +RL   L+  Q+F++      E    + H      +LVCGGDGT + V + ++   ++  PP ++I+PLGTGND++RV+ W  G          S+      + ++ +D+  A    +DRW + + E   + D+  + VK+M  Y+G+G D KVA DFH  R+  P  F S  +NK  YA  G +D   RSC +LP  V L  DGK + +P   E  IVLNI S+ GGV+LW         +  F      D  LEVV ISG  HLG+++VGL R  RLAQ K +R+   S  P QVDGEPW  P   L + H GQ  +L+  S+E   +   ++ +        G +DAA K  LL     RL
Sbjct:  326 EIIDLPQDSRPLLVFINGKSGGRNGPSLRRRLNMLLNPIQIFELSASQGPEVGLQFFHNVKHFRILVCGGDGTVAWVLDAIEKQNYE-SPPPVSILPLGTGNDLSRVMRWGGG---------LSSVEGQGGICALLNDVDHAAVTVLDRWNVAIKEKNGAEDQCTKQVKFMTNYIGVGCDAKVAYDFHTTREEKPDKFCSQFVNKLIYAREGAKDIMDRSCSDLPWHVSLEVDGKNVEIPEDAEGVIVLNIPSYMGGVDLWQN---DNEHDDDFGLQSMHDKMLEVVCISGTWHLGKLQVGLSRAHRLAQGKVIRLHLHSSFPVQVDGEPWIQPPGCLEISHRGQMFMLRRTSEEPTGHAAAIMSEVLMNAECNGVIDAAQKRLLLHEIALRL 705          

BLAST of EWM25228.1 vs. NCBI_GenBank
Match: gi|1208520244|gb|OWM76348.1| (hypothetical protein CDL15_Pgr028218 [Punica granatum])

HSP 1 Score: 242.662 bits (618), Expect = 1.406e-67
Identity = 146/391 (37.34%), Postives = 220/391 (56.27%), Query Frame = 0

Query:  215 RIKTLPPSVTPLIAIVNSKSGGRQGKNLFKRLRAALSRAQVFDIQKVDL-KEALSLYCHLPNSCTLLVCGGDGTASRVFEVVDGMEWKHGPPKIAIVPLGTGNDIARVLDWNLGHDWSGGYFPWSNDAADANLLSVFSDLTRAMERKMDRWELRMTEAVPSSDRHRQPVKYMLGYLGIGVDGKVALDFHKLRDRAPYLFLSPTLNKFYYALMGLRDFFVRSCKNLPDKVELWCDGKPIVLPPQTESFIVLNINSHAGGVELWP-EYLMGGGMEGAFKPSRFDDGYLEVVAISGVLHLGRIRVGLDRPLRLAQAKEVRIRTKSFLPGQVDGEPWRLPRCELTLRHNGQAPVLQHVSKELLQYNEWLVGQGKLDAAGKDQLLQAFKRRLQVSQ 603
            +I  L P V PL+  +N+KSGG+ G +L +RL   L+  QVF +         L ++ +LPN   +LVCGGDGT + V + ++   ++  PP +AI+PLGTGND++RVL W  G         +S+  +   L ++  D+  A    +DRW++ +TE       H+   K+M+ YLGIG D KVA +FH LR+  P  F +  +NK  YA  G +D   R+C +LP +V L  DGK I +P  +E  IVLNI S+ GGV+LW  EY      +  F      D  LEVV + G  HLG+++VGL +  RLAQ K +RI T S  P Q+DGEP+    C L ++H+GQ  +L+   +E   +   ++ +   DA  K  ++ A ++++ + Q
Sbjct:  323 QIVDLRPDVRPLLVFINAKSGGQLGHSLRRRLNMLLNPVQVFALSASQGPAVGLEMFRNLPNF-RVLVCGGDGTVAWVLDAIEKQNFE-SPPPVAILPLGTGNDLSRVLQWGRG---------FSSGDSQGGLTTLLHDINHAAITMLDRWKVNITEEKQDGGPHKVHSKFMMNYLGIGCDAKVAYEFHTLREENPEKFYNRFVNKLRYAKEGAKDIMDRACADLPWQVWLEVDGKDIPIPTDSEGLIVLNIGSYMGGVDLWQNEY----EHDDEFSLQCMHDQMLEVVCVCGAWHLGKLQVGLSQARRLAQGKVIRIHTSSPFPVQIDGEPFIQQPCCLEIKHHGQMFMLRRALEEPRGHAAAIMTEVLADAECKG-IINASQKKILLQQ 697