EWM25297.1, cds5376 (CDS) Nannochloropsis gaditana

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|585107047|gb|EWM25297.1| (splicing factor 3a [Nannochloropsis gaditana])

HSP 1 Score: 1117.84 bits (2890), Expect = 0.000e+0
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDAFPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVPAGKKNAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDAFPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVPAGKKNAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL
Sbjct:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDAFPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVPAGKKNAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546          

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|553180466|ref|XP_005853408.1| (splicing factor 3A subunit 3 [Nannochloropsis gaditana CCMP526] >gi|422295652|gb|EKU22951.1| splicing factor 3A subunit 3 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1114.75 bits (2882), Expect = 0.000e+0
Identity = 545/546 (99.82%), Postives = 545/546 (99.82%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDAFPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVPAGKKNAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDAFPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVPAGKKNAAWARYVHNLYNYL SFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL
Sbjct:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDAFPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVPAGKKNAAWARYVHNLYNYLHSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546          

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|298710033|emb|CBJ31751.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 468.774 bits (1205), Expect = 2.681e-157
Identity = 276/567 (48.68%), Postives = 355/567 (62.61%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDAFPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTP--KWDYQTYLSRLADFSVVPAGKK--NAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWP-------VPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKK----------QCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MS   LERAR  HED+E YE  I+  LE KPRT KERV QQHR++NL +    RS+ L  IYED  G+M+EE+A++RG+    +FY R+     YH  HP +PV   P ++ E E +V FSGEE++GK+ DL    + +  LPQ    + DY  +L  L  F  +P  +K    A+A ++ +L  YL  F+ RTQPL++++E+L G+ D           ++FE  W+AG LPG W          A     + GA   + +DL+ F  A EL  LGMDRLK  L+A+G+KCGGTLE+RA RLF+ KGKK EEID         K    +     SNG   G G D         G            Q A  E ++  L+ EV  + +E+TK+ V+KK TRT EE + E+++EE+G LPE        +E+EEEE DE+GPIYNPLNLPLG+DGKPIPYWLYKLHGLGVE+KCEICG+ SY GRR FDRHFQEWRHAHGMRCL IPNTKHFHDI  I+DA  LY +IK+ L  E ++ E  EE+ED EGNVLNRRT++DLARQGL+
Sbjct:    1 MSSSLLERARGFHEDLEIYERGIIDQLETKPRTQKERVAQQHRIANLCDGSTERSQGLHEIYEDTDGSMREEVASMRGQKVMDTFYDRLKETWSYHTHHPGLPVTFGPNLEAETEVKVQFSGEELYGKFFDLHGMFVRWVNLPQCKDKQLDYSQFLQALGRFEDIPEQEKLRGKAYAGFLADLREYLGGFLTRTQPLVDLDEVL-GDTD-----------SSFEEAWEAGTLPG-WTSGRAAAAAAAAAAAGKGGAAAPRVLDLKKFHDADELRALGMDRLKEALQAIGLKCGGTLEQRADRLFSVKGKKPEEID------QKLKAKGKKEGKGVSNGVAPGHGADPNANGPAGGGGGRGGGKEERRKQLAALETKVKALL-EVQTDTLESTKRQVDKKHTRTVEERDQEIQEEEQGALPE-------FNEEEEEESDEEGPIYNPLNLPLGWDGKPIPYWLYKLHGLGVEFKCEICGDFSYKGRRNFDRHFQEWRHAHGMRCLGIPNTKHFHDIVLIQDARDLYAKIKDSLDKEQWNPEDNEEYEDGEGNVLNRRTYEDLARQGLM 540          

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|901797759|gb|KMZ59769.1| (Splicing factor 3A subunit 3, putative, expressed [Zostera marina])

HSP 1 Score: 385.185 bits (988), Expect = 5.179e-125
Identity = 224/556 (40.29%), Postives = 325/556 (58.45%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDA-----FPSFYARVDHAKEYHQKHPDVPV---KHHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVPAGKK-NAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLA-GNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MS   LE  RA HE++E  E  +V  L+ +P + +ER++Q HRV N+IE +   ++ L  IYEDV G  KEE++ L G+ A     F +FY ++   ++YH++HP   V           + EPR+ FSGEE FG+YLD+   +  +       + +Y ++L   + F  + +  K    ++ Y++ L  YL+SF QRT+PL +V  +              K E  FE LW  GK+  GW   A         E+   +DL  + T +EL  +G ++LK  L ALG+K GGT+++RA RLF TK K  E++D K  A G+G  ++K    G  SN   +G               +  A  E +I  L  E+L+E++  TK+ +EKKQ  T EE E E   E+      ++ E E +DED++        IYNPL LP+G+DGKPIPYWLYKLHGLG E+KCEICGNHSYWGRRAF+RHF+EWRH HGMRCL IPN K+F++IT+I+++  L++RI+ +     +  ++EEE+ED EGN+ N++T+ DL RQGL+
Sbjct:    1 MSSTLLEVTRAAHEEVERMERLVVQDLQREPVSGRERLFQNHRVRNMIETVVTTTEKLVNIYEDVDGARKEEISVLGGQVASGTNLFSTFYDKLKEIRDYHRRHPTAYVLDASEEYEEMLKEEPRIEFSGEEAFGRYLDMHELYNMYINSKFGKQIEYSSFLETFSQFQKIASNLKLTRQYSDYLNYLLEYLISFFQRTEPLQDVGRMFS------------KVETEFEELWTDGKM-SGWECKAMNNENIPSQESL--IDLDYYNTVEELMEVGPEKLKEALAALGLKVGGTVKQRADRLFLTKDKPLEQLDRKYFAKGSGVFQNK----GSASNSKDKG-------------SMRFIALMECKIQKLC-ELLKEIIIQTKENIEKKQALTYEEMEAEREDED------VQAESESDDEDQQ--------IYNPLKLPMGWDGKPIPYWLYKLHGLGQEFKCEICGNHSYWGRRAFERHFKEWRHQHGMRCLNIPNNKNFNEITSIEESKKLWERIQARQGVNKWRPDLEEEYEDREGNIFNKKTYTDLVRQGLI 509          

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|698520651|ref|XP_009757142.1| (PREDICTED: splicing factor 3A subunit 3 [Nicotiana sylvestris] >gi|698520653|ref|XP_009757143.1| PREDICTED: splicing factor 3A subunit 3 [Nicotiana sylvestris])

HSP 1 Score: 380.178 bits (975), Expect = 4.250e-123
Identity = 229/556 (41.19%), Postives = 320/556 (57.55%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDA-----FPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEA----EPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVPA-GKKNAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MS   LE  RA+HE++E  E  +V  L+ +P T K+R+ Q HRV N+IEQI   +  L  IYED  G  K+E+A L G+ A     F +FY R+   +EYH++HP   V        E     EP++ FSGEE  G+YLDL     E+Y      + +Y  YL   +    +P   K    +  Y+  L  YL+ F +RT+PL +++ I              K  + FE  W  GK+ G W   +Q  G     +N   +DL  + T +EL + G D+LK  L ALG+K GGT+++RA RLF TK    E++D K  A  G +K +  G  P        VG          D  K+ A  E +I  L  ++L E +  TK+ VEKKQ  T EE E E  +++      ++ E E ++ED++        IYNPL LP+G+DGKPIPYWLYKLHGLG E+KCEICGNHSYWGRRA++RHF+EWRH HGMRCL IPNTK+F++IT+I++A  L++RI+E+     +  ++EEE+ED EGN+ N++T+ DL RQGL+
Sbjct:    1 MSSTLLEVTRASHEEVERLERLVVKDLQTEPATNKDRLHQSHRVRNMIEQIIDTTHKLVDIYEDKDGARKDEIAALGGQTATGTNIFSAFYDRLKEIREYHRRHPAARVVIDASDDYEQLLKEEPQIEFSGEEGSGRYLDLHELFNEYYNSKFGARIEYTAYLDVFSQPQKIPCKAKSTRPYREYLQKLLEYLIYFFERTEPLQDLDRIFS------------KVTSEFEEEWTIGKVEG-WENSSQENG--SAPQNHTAIDLNSYSTVEELMIEGPDKLKEALGALGLKTGGTIQQRAERLFLTKHTPLEKLDKKHFA-KGSRKPEQNGVMPAPQ-----VG----------DDVKEIALMEAKIRKLC-DLLDETIVRTKENVEKKQALTYEEMEAEREEDD------VQAESESDEEDQQ--------IYNPLKLPMGWDGKPIPYWLYKLHGLGQEFKCEICGNHSYWGRRAYERHFKEWRHQHGMRCLGIPNTKNFNEITSIEEAKQLWERIQEKQGLNKWRPDLEEEYEDQEGNIYNKKTYTDLQRQGLI 510          

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|1025191665|ref|XP_016475308.1| (PREDICTED: splicing factor 3A subunit 3-like [Nicotiana tabacum] >gi|1025191668|ref|XP_016475309.1| PREDICTED: splicing factor 3A subunit 3-like [Nicotiana tabacum])

HSP 1 Score: 380.178 bits (975), Expect = 5.814e-123
Identity = 229/556 (41.19%), Postives = 320/556 (57.55%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDA-----FPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEA----EPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVP-AGKKNAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MS   LE  RA+HE++E  E  +V  L+ +P T K+R+ Q HRV N+IEQI   +  L  IYED  G  K+E+A L G+ A     F +FY R+   +EYH++HP   V        E     EP++ FSGEE  G+YLDL     E+Y      + +Y  YL   +    +P   K    +  Y+  L  YL+ F +RT+PL +++ I              K  + FE  W  GK+ G W   +Q  G     +N   +DL  + T +EL + G D+LK  L ALG+K GGT+++RA RLF TK    E++D K  A  G +K +  G  P        VG          D  K+ A  E +I  L  ++L E +  TK+ VEKKQ  T EE E E  +++      ++ E E ++ED++        IYNPL LP+G+DGKPIPYWLYKLHGLG E+KCEICGNHSYWGRRA++RHF+EWRH HGMRCL IPNTK+F++IT+I++A  L++RI+E+     +  ++EEE+ED EGN+ N++T+ DL RQGL+
Sbjct:    1 MSSTLLEVTRASHEEVERLERLVVKDLQTEPATNKDRLHQSHRVRNMIEQIIDTTHKLVDIYEDKDGARKDEIAALGGQTATGTNIFSAFYDRLKEIREYHRRHPAARVVIDASDDYEQLLKEEPQIEFSGEEGSGRYLDLHELFNEYYNSKFGARIEYTAYLDVFSQPQKIPRKAKSTRPYREYLQKLLEYLIYFFERTEPLQDLDRIFS------------KVTSEFEEEWTIGKVEG-WENSSQENG--SAPQNHTAIDLNSYSTVEELMIEGPDKLKEALGALGLKTGGTIQQRAERLFLTKHTPLEKLDKKHFA-KGSRKPEQNGVMPAPQ-----VG----------DDVKEIALMEAKIRKLC-DLLDETIVRTKENVEKKQALTYEEMEAEREEDD------VQAESESDEEDQQ--------IYNPLKLPMGWDGKPIPYWLYKLHGLGQEFKCEICGNHSYWGRRAYERHFKEWRHQHGMRCLGIPNTKNFNEITSIEEAKQLWERIQEKQGLNKWRPDLEEEYEDQEGNIYNKKTYTDLQRQGLI 510          

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|697130347|ref|XP_009619235.1| (PREDICTED: splicing factor SF3a60 homolog [Nicotiana tomentosiformis] >gi|697130349|ref|XP_009619236.1| PREDICTED: splicing factor SF3a60 homolog [Nicotiana tomentosiformis])

HSP 1 Score: 379.407 bits (973), Expect = 9.053e-123
Identity = 229/556 (41.19%), Postives = 321/556 (57.73%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDA-----FPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEA----EPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVP-AGKKNAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MS   LE  RA+HE++E  E  +V  L+ +P T K+R+ Q HRV N+IEQI   +  L  IYED  G  K+E+A L G+ A     F +FY R+   +EYH++HP   V        E     EP++ FSGEE  G+YLDL     E+Y      + +Y  YL   +    +P   K    +  Y+  L  YL+ F +RT+PL +++ I              K  + FE  W  GK+ G W   +Q  G     +N   +DL  + T +EL + G D+LK  L ALG+K GGT+++RA RLF TK    E++D K  A  G +K +  G  P +      VG          D  K+ A  E +I  L  ++L E +  TK+ VEKKQ  T EE E E  +++      ++ E E ++ED++        IYNPL LP+G+DGKPIPYWLYKLHGLG E+KCEICGNHSYWGRRA++RHF+EWRH HGMRCL IPNTK+F++IT+I++A  L++RI+E+     +  ++EEE+ED EGN+ N++T+ DL RQGL+
Sbjct:    1 MSSTLLEVTRASHEEVERLERLVVKDLQTEPATNKDRLHQSHRVRNMIEQIIDTTHKLVDIYEDKDGARKDEIAALGGQTATGTNVFSAFYDRLKEIREYHRRHPAARVVIDASDDYEQLLIEEPQIEFSGEEGSGRYLDLHELFNEYYNSKFGARIEYTAYLDVFSQPQKIPRKAKSTRPYREYLQKLLEYLIYFFERTEPLQDLDRIFS------------KVTSEFEEEWTIGKVEG-WENNSQENG--SAPQNHTVIDLNSYSTVEELTIEGPDKLKEALGALGLKTGGTIQQRAERLFFTKHTPLEKLDRKHFA-KGSRKPEQNGVMPAAQ-----VG----------DDVKEIALMEAKIRKLC-DLLDETIVRTKENVEKKQALTYEEMEAEREEDD------VQAESESDEEDQQ--------IYNPLKLPMGWDGKPIPYWLYKLHGLGQEFKCEICGNHSYWGRRAYERHFKEWRHQHGMRCLGIPNTKNFNEITSIEEAKQLWERIQEKQGLNKWRPDLEEEYEDQEGNIYNKKTYTDLQRQGLI 510          

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|565367837|ref|XP_006350561.1| (PREDICTED: splicing factor 3A subunit 3 [Solanum tuberosum])

HSP 1 Score: 378.252 bits (970), Expect = 3.106e-122
Identity = 232/560 (41.43%), Postives = 329/560 (58.75%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDA-----FPSFYARVDHAKEYHQKHP------DVPVKHHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVP-AGKKNAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGA--ENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MS   LE  RA+HE++E +E  IV  L+ +P T K+R+ Q HRV N+IEQI   +  L  IYED  G  K+E+A L G+ A     F +FY R+   +EYH++HP      D   ++   ++EE  P++ FSGEE  G+YLDL     E+         +Y  YL+  ++   +P   K    +  Y+  L  YL+ F +RT+PL +++ I              K  + FE  W  GK+ G W    +  G+E G+  EN   +DL  + TA+EL V+G ++LK  L ALG+K GGT+++RA RLF TK    E++D K  A   +K        P  NG    V T   G        K+ A  E +I  L  ++L E +  T++ VEKKQ  T EE E E  +++      ++ E E +DED++        IYNPL LP+G+DGKPIPYWLYKLHGLG E+KCEICGNHSYWGRRA++RHF+EWRH HGMRCL IPNTK+F++IT+I++A  L++RI+E+     +  ++EEE+ED EGN+ N++T+ DL RQGL+
Sbjct:    1 MSSTLLEVTRASHEEVERFERLIVKDLQTEPATNKDRLHQSHRVRNMIEQIIDTTHKLVDIYEDKDGARKDEIAALGGQTATGTNVFSAFYDRLKEIREYHRRHPAARAIIDTGDEYEQLLKEE--PQIEFSGEEGSGRYLDLHELFNEYINSKFGEPIEYTAYLNVFSEPQKIPHKAKTTRQYLEYLQKLLEYLVYFFERTEPLQDLDRIFS------------KVTSEFEEEWTLGKVEG-W----ENSGQENGSAPENQTVIDLDYYSTAEELMVVGPEKLKEALGALGLKTGGTIQQRAERLFLTKNISLEKLDRKHFAKGSRK--------PEQNG---VVATPHVGDV------KEIALLEAKIRRLC-DLLDETIVRTRENVEKKQALTYEEMEAEREEDD------VQAESESDDEDQQ--------IYNPLKLPMGWDGKPIPYWLYKLHGLGQEFKCEICGNHSYWGRRAYERHFKEWRHQHGMRCLGIPNTKNFNEITSIEEAKLLWERIQERQGVNKWRPDLEEEYEDQEGNIYNKKTYTDLQRQGLI 509          

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|1025410382|ref|XP_016439391.1| (PREDICTED: splicing factor 3A subunit 3-like [Nicotiana tabacum] >gi|1025410386|ref|XP_016439392.1| PREDICTED: splicing factor 3A subunit 3-like [Nicotiana tabacum])

HSP 1 Score: 377.096 bits (967), Expect = 8.287e-122
Identity = 228/556 (41.01%), Postives = 319/556 (57.37%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATLRGRDA-----FPSFYARVDHAKEYHQKHPDVPVKHHPPVQEEA----EPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVP-AGKKNAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MS   LE  RA+HE++E  E  +V  L+ +P T K+R+ Q HRV N+IEQI   +  L  IYED  G  K+E+A L G+ A     F +FY R+   +EYH++HP   V        E     EP++ FSGEE  G+YLDL     E+Y      + +Y  YL   +    +P   K    +  Y+  L  YL+ F +RT+PL +++ I              K  + FE  W  GK+ G W   +Q  G     +N   +DL  + T +EL + G D+LK  L ALG+K GGT+++RA R F TK    E++D K  A  G +K +  G  P        VG          D  K+ A  E +I  L  ++L E +  TK+ VEKKQ  T EE E E  +++      ++ E E ++ED++        IYNPL LP+G+DGKPIPYWLYKLHGLG E+KCEICGNHSYWGRRA++RHF+EWRH HGMRCL IPNTK+F++IT+I++A  L++RI+E+     +  ++EEE+ED EGN+ N++T+ DL RQGL+
Sbjct:    1 MSSTLLEVTRASHEEVERLERLVVKDLQTEPATNKDRLHQSHRVRNMIEQIIDTTHKLVDIYEDKDGARKDEIAALGGQTATGTNVFSAFYDRLKEIREYHRRHPAARVVIDASDDYEQLLIEEPQIEFSGEEGSGRYLDLHELFNEYYNSKFGARIEYTAYLDVFSQPQKIPRKAKSTRPYREYLQKLLEYLIYFFERTEPLQDLDRIFS------------KVTSEFEEEWTIGKVEG-WENNSQENG--SAPQNHTVIDLNSYSTVEELTIEGPDKLKEALGALGLKTGGTIQQRAERFFLTKHTPLEKLDRKHFA-KGSRKPEQNGVMPAPQ-----VG----------DDVKEIALMEAKIRKLC-DLLDETIVRTKENVEKKQALTYEEMEAEREEDD------VQAESESDEEDQQ--------IYNPLKLPMGWDGKPIPYWLYKLHGLGQEFKCEICGNHSYWGRRAYERHFKEWRHQHGMRCLGIPNTKNFNEITSIEEAKQLWERIQEKQGLNKWRPDLEEEYEDQEGNIYNKKTYTDLQRQGLI 510          

BLAST of EWM25297.1 vs. NCBI_GenBank
Match: gi|294462630|gb|ADE76861.1| (unknown [Picea sitchensis])

HSP 1 Score: 375.555 bits (963), Expect = 2.476e-121
Identity = 234/554 (42.24%), Postives = 319/554 (57.58%), Query Frame = 0

Query:    1 MSGLTLERARANHEDIEAYEAAIVAILEDKPRTAKERVWQQHRVSNLIEQIQGRSKDLEGIYEDVHGTMKEELATL-----RGRDAFPSFYARVDHAKEYHQKHPDVPVK--HHPPVQEEAEPRVSFSGEEVFGKYLDLQASHMEFYALPQTPKWDYQTYLSRLADFSVVPAGKK-NAAWARYVHNLYNYLLSFIQRTQPLLNVEEILDGNVDGGEEGATKKAEAAFEALWQAGKLPGGWPVPAQGGGEEKGAENTKEVDLRGFRTAKELEVLGMDRLKAGLKALGMKCGGTLEERAARLFATKGKKKEEIDPKLLAGNGKKKSKAEGNGPTSNGNGQGVGTDKEGGKGGEDGKKQCARWEFRIDLLVKEVLREVVEATKKFVEKKQTRTKEETETELRQEEEGLLPELRGEGEEEDEDEEEEEDEDGPIYNPLNLPLGYDGKPIPYWLYKLHGLGVEYKCEICGNHSYWGRRAFDRHFQEWRHAHGMRCLKIPNTKHFHDITTIKDALALYDRIKEQLKGEVYDAEVEEEFEDSEGNVLNRRTFQDLARQGLL 546
            MS   LE  R  HE++E  E  IV  L+ +P T KER+ Q HRV N+++ I   ++ L  +YED   + K+E+A L      G++ F +FY R+   +EYH++HP   V     P    + EPRV FSGEE FG+YLD+   + E+         DY  +L        +P   K    +  Y  +L  YL+SF QRTQPL ++++I              K EA FE  W AG +  GW     G G+    ++   +DL  + +  EL  LG ++LK  L ALG+K GGT+++RA RLF TK    EE+D         +K  A+G+ P++        TD E  +      K+ A  E ++  LV E L E +E TK  VEKKQ  T EE E E                EEE + E E ++ED  IYNPL LP+G+DGKPIPYWLYKLHGLG E+KCEICGNHSYWGRRAF+RHF+EWRH HGMRCL IPNTK+F++IT+IK+A  L++RI+E+     +  ++EEE+ED EGN+ N++T+ DL RQGL+
Sbjct:    1 MSSTLLEVTRGAHEEVERLERLIVKDLQREPETNKERMHQNHRVRNMVDAIMSTTQKLIEVYEDSDHSRKDEIAALGGQGVSGQNVFSAFYDRLKEIREYHRRHPIARVVDLEDPEELLKEEPRVEFSGEEAFGRYLDMHELYNEYVNSKFGQLIDYAAFLEEFPQTHNIPRTHKLTRQYKEYSRHLLEYLVSFFQRTQPLQDLDKIFS------------KVEAEFEEHW-AGGMVQGWEDKGLGNGQPSTIQDF--IDLDYYSSVDELVELGPEKLKQALAALGLKTGGTVQQRAERLFLTKVTPLEELD---------RKHFAKGSRPSARR------TDDEILQLT--ASKEVALMEVKMKRLV-EFLHETLEETKANVEKKQALTYEEMEAE--------------REEEEIQAESESDEEDQQIYNPLKLPMGWDGKPIPYWLYKLHGLGQEFKCEICGNHSYWGRRAFERHFKEWRHQHGMRCLGIPNTKNFNEITSIKEAKFLWERIQERQGLNKWRPDLEEEYEDQEGNIYNKKTYTDLQRQGLI 507