EWM25332.1, cds5334 (CDS) Nannochloropsis gaditana

Overview
NameEWM25332.1
Unique Namecds5334
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002465.1:772821..773582 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM25332.1
ProductTRAM, LAG1 and CLN8 lipid-sensing domain containing protein
Orig transcript idgnl|cribi|Naga_100196g3.3056.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002465.1supercontigCM002465.1:772821..773582 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: INTERPRO
TermDefinition
IPR006634TLC-dom
Homology
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|585107091|gb|EWM25332.1| (TRAM, LAG1 and CLN8 lipid-sensing domain containing protein [Nannochloropsis gaditana])

HSP 1 Score: 518.079 bits (1333), Expect = 0.000e+0
Identity = 253/253 (100.00%), Postives = 253/253 (100.00%), Query Frame = 0
Query:    1 MKTTDLLAAIQLYVDILADRWPGLVLSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPCEIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKKKGR 253
            MKTTDLLAAIQLYVDILADRWPGLVLSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPCEIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKKKGR
Sbjct:    1 MKTTDLLAAIQLYVDILADRWPGLVLSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPCEIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKKKGR 253          
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|553182739|ref|XP_005853778.1| (hypothetical protein NGA_0438500 [Nannochloropsis gaditana CCMP526] >gi|422295289|gb|EKU22588.1| hypothetical protein NGA_0438500 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 512.686 bits (1319), Expect = 0.000e+0
Identity = 250/253 (98.81%), Postives = 252/253 (99.60%), Query Frame = 0
Query:    1 MKTTDLLAAIQLYVDILADRWPGLVLSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPCEIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKKKGR 253
            MKTTDLLAAIQ YVDILADRWPGL+LSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPC+IGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKKKGR
Sbjct:    1 MKTTDLLAAIQPYVDILADRWPGLILSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPCKIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKKKGR 253          
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|553187328|ref|XP_005854585.1| (hypothetical protein NGA_0175400 [Nannochloropsis gaditana CCMP526] >gi|422294466|gb|EKU21766.1| hypothetical protein NGA_0175400 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 507.294 bits (1305), Expect = 0.000e+0
Identity = 247/251 (98.41%), Postives = 250/251 (99.60%), Query Frame = 0
Query:    1 MKTTDLLAAIQLYVDILADRWPGLVLSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPCEIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKKK 251
            MKTTDLLAAIQ YVDILADRWPGL+LSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPC+IGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKK+
Sbjct:    1 MKTTDLLAAIQPYVDILADRWPGLILSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPCKIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKKR 251          
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|553183464|ref|XP_005853901.1| (hypothetical protein NGA_0463400 [Nannochloropsis gaditana CCMP526] >gi|422295156|gb|EKU22455.1| hypothetical protein NGA_0463400 [Nannochloropsis gaditana CCMP526] >gi|585102711|gb|EWM22253.1| TRAM, LAG1 and CLN8 lipid-sensing domain containing protein [Nannochloropsis gaditana])

HSP 1 Score: 159.073 bits (401), Expect = 6.079e-44
Identity = 84/232 (36.21%), Postives = 134/232 (57.76%), Query Frame = 0
Query:   23 GLVLSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPCEIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSE-GKDKGGEKKKGR 253
            G+ +S   WS++FG+V VLP   G +F ++ R V+I HAF +  L   L  +  P EIGG NT  Q IV+NV+ G+F+YD ++  +       +D M  FHH + L G      + RSGA++   LF+ E++NPFM+  +L RE+G+ D  +A  N+A+FA+  TL   +  P L    L+ P++H + +A A+G++ V+ +WF K    ++K +  ++ G  K G K   R
Sbjct:   28 GVCVSATIWSLLFGIVQVLPIFKGRTFGYKTRVVAIFHAFTTMTLAYRLSFMCRPLEIGGINTPGQDIVVNVAGGFFLYDLISWIIYGYVIAKYDWMQLFHHASCLVGSWACWSSGRSGADVTFALFVAELANPFMYLRYLLREVGWKDTTLARTNQAIFALAMTLTIPILAPILACVILMEPKSHPLHQAAAVGLVVVNMMWFFKF---MTKYWAAAQTGPLKTGRKASPR 256          
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|1026765301|gb|OAE26028.1| (hypothetical protein AXG93_4601s1270 [Marchantia polymorpha subsp. ruderalis])

HSP 1 Score: 151.369 bits (381), Expect = 1.150e-41
Identity = 94/229 (41.05%), Postives = 129/229 (56.33%), Query Frame = 0
Query:   17 LADRWPGLVLSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVL-LETPCE-IGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINE----------RSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAML 233
            +AD W GL + TVAW   FG+V  L      S +F NR VS++H F++AYL +  V     P + IGGP++  Q+  +  S  YF+YD+  C +   R    D  N  HH+ ++ GL  G + +          + G EL  CL+LME+SNPFMH   LF+E G  D P+  AN   F +IFTLAR+  GP++ Y+TL S  T  V+K GA+GI  VS  WF KI  M+
Sbjct:    1 MAD-WQGLAVGTVAWGAGFGIVTALLG-KKKSGEFCNRCVSMVHVFVAAYLCMASVSDWSRPLDGIGGPSSALQMRAIKTSLAYFVYDFFCCLVVFPR----DIGNAVHHIITIMGLGYGFMTQTCPNEAKDVMQCGTELVACLWLMELSNPFMHARELFKEFGIKDTPLNMANDLSFVVIFTLARMGVGPYVVYHTLNSQSTP-VVKFGAVGIQVVSVFWFYKIARMV 222          
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|255071719|ref|XP_002499534.1| (predicted protein [Micromonas commoda] >gi|226514796|gb|ACO60792.1| predicted protein [Micromonas commoda])

HSP 1 Score: 151.754 bits (382), Expect = 1.385e-41
Identity = 89/226 (39.38%), Postives = 126/226 (55.75%), Query Frame = 0
Query:   28 TVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVLLETPCE-----IGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAML----SKAFGGSEGKD 244
            TV W ++F +VC        S DF NR VSI+HA ++ Y + V    E   +     IGG NT  Q + + +S  YF YD + C L        D M+  HH+ ++ GL +G++N RSGAEL  CLFLMEVSNP +H   +F E+G   + +A  N  +FA++F + R+V GP L + TL SP   +++KAG  GILAVS  W   I+ ++     K  GG + K 
Sbjct:   26 TVFWGLMF-VVCRFALFKKYSADFSNRVVSIVHAVVAIYYSYVT--FENGWDGMFDNIGGANTEAQTLCMAISLSYFTYDLIYCALGG------DFMSVVHHMFTMGGLASGVLNGRSGAELVACLFLMEVSNPSLHLRTVFIEMGLKTSMLASINSLVFALMFLVCRLVIGPPLVWKTLASPDNDYIVKAGGFGILAVSVFWGMSIIRIIVKEVKKLMGGGKAKQ 242          
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|308805829|ref|XP_003080226.1| (unnamed protein product [Ostreococcus tauri] >gi|116058686|emb|CAL54393.1| TRAM/LAG1/CLN8 homology domain [Ostreococcus tauri] >gi|1199292410|gb|OUS47498.1| TLC domain-domain-containing protein [Ostreococcus tauri])

HSP 1 Score: 149.058 bits (375), Expect = 1.756e-40
Identity = 89/229 (38.86%), Postives = 131/229 (57.21%), Query Frame = 0
Query:   28 TVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLS-AYLTLVL----VLLETPCEIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGGEKKK 251
            T+AW+ ++ L   +  +   + DF NR VS++HA ++  Y    L     LLE    +GG NT   +  + +S GYF+YD + C LN+      +  N  HH+ ++ GL +G++  RSG EL  CLFLMEVSNP +H   L REL   D+ +A  N  +FA++F   R+V GP L Y T+V+    +++KAGALGIL VS +W  KI+ M+ +    +  K  GG K K
Sbjct:   33 TLAWTALYVLFRFV-LLRKRTADFNNRLVSLIHALVAMKYCVACLPTWGALLE---NVGGKNTSAHLDCITMSLGYFVYDLIYCVLNN------EIENVIHHMFTVGGLASGVVTGRSGPELVGCLFLMEVSNPSLHLRSLLRELDMKDSVLASVNDLIFALLFLFCRLVVGPPLVYKTVVNKDNTYLVKAGALGILIVSLMWAWKIIMMIVR----TSKKLAGGSKPK 247          
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|1021527767|ref|XP_016207416.1| (transmembrane protein 136 [Arachis ipaensis])

HSP 1 Score: 144.436 bits (363), Expect = 8.057e-39
Identity = 87/226 (38.50%), Postives = 124/226 (54.87%), Query Frame = 0
Query:   24 LVLSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVL-LETP-CEIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGG 247
            +V+  V+WS  F  +     +   SFDF NR +S +HA L+  L  + V   + P C +   ++  Q+ VL VS  Y IYD V C   +  +   D  N  HHL S+ GL  GL  ++ G+E+  C+++ E+S+PF+H   L +ELGY D P+  A   LFA IFT AR+V GP+LTY TL S    ++IKA ALG+  VS  WF KI+ M+       +     G
Sbjct:    9 IVVGVVSWSSAF--LVTRRILQKRSFDFSNRVISTIHAILAVTLASISVQDWKCPICPVASNSSLTQMQVLAVSVSYLIYDLVCCLFGE--RVSMD--NTIHHLVSILGLGAGLCYQKCGSEMVACVWITEMSSPFLHLRELLKELGYRDTPLNLAADLLFAAIFTFARMVAGPYLTYVTL-SANNPFLIKATALGLQLVSAFWFFKILRMVKYKLTKKKSTSING 227          
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|1012186732|ref|XP_015969640.1| (transmembrane protein 136 isoform X2 [Arachis duranensis])

HSP 1 Score: 142.895 bits (359), Expect = 3.034e-38
Identity = 86/226 (38.05%), Postives = 123/226 (54.42%), Query Frame = 0
Query:   24 LVLSTVAWSVVFGLVCVLPAISGTSFDFRNRTVSILHAFLSAYLTLVLVL-LETP-CEIGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGG 247
            +V+  V+WS  F  +     +   SFDF NR +S +HA L+  L  + V   + P C +   ++  Q+ VL VS  Y IYD V C   +  +   D  N  HHL S+ GL  GL  ++ G+E+  C+++ E+S+PF+H   L +ELGY D P+  A   LFA IFT AR+V GP+LTY TL S    ++IKA A G+  VS  WF KI+ M+       +     G
Sbjct:    9 IVVGVVSWSSAF--LVTRRILQKRSFDFSNRVISTIHAILAVTLASISVQDWKCPICPVASNSSLTQMQVLAVSVSYLIYDLVCCLFGE--RVSMD--NTIHHLVSILGLGAGLCYQKCGSEMVACVWITEMSSPFLHLRELLKELGYRDTPLNLAADLLFAAIFTFARMVAGPYLTYVTL-SANNPFLIKATAFGLQLVSAFWFYKILRMVKYKLTKKKSTSING 227          
BLAST of EWM25332.1 vs. NCBI_GenBank
Match: gi|168015700|ref|XP_001760388.1| (predicted protein [Physcomitrella patens] >gi|162688402|gb|EDQ74779.1| predicted protein [Physcomitrella patens])

HSP 1 Score: 141.739 bits (356), Expect = 4.051e-38
Identity = 90/223 (40.36%), Postives = 119/223 (53.36%), Query Frame = 0
Query:   28 TVAWSVVFGLVCVL-PAISGTSFDFRNRTVSILHAFLSAYLTLVLVL-LETPCE-IGGPNTRNQIIVLNVSTGYFIYDYVACTLNDVRKRHFDTMNFFHHLASLAGLLTGLINERSGAELGMCLFLMEVSNPFMHTIHLFRELGYNDAPVAEANKALFAIIFTLARVVGGPFLTYYTLVSPRTHWVIKAGALGILAVSFLWFSKIVAMLSKAFGGSEGKDKGG 247
            T AW+ V+ L  VL PA S    DF NR VS++H F+S Y     V     P + +G  +T  Q++ L VS  YF YD + C +    +  F    F HH+ ++ GL  G   + SG EL  CL LMEVSNPFMH   L +EL   D+ +  AN  +FA+IFT+ARV  GP + Y  L+S  T + +K GA+GI  VS LWF KI  M+           K  
Sbjct:    2 TAAWTFVYVLSRVLFPARSK---DFCNRIVSLIHVFVSCYFCSKSVADWSRPLDGVGAASTYPQMLALTVSLAYFTYDTLCCLV----ELPFSLDVFMHHVLTILGLGFGYERQISGTELVACLMLMEVSNPFMHARELLKELNLKDSSMNLANDVVFALIFTVARVFIGPIVVYKCLLSSTTPFAVKVGAVGIQVVSLLWFYKIARMVVYKLSKKPKTRKAA 217          
The following BLAST results are available for this feature:
BLAST of EWM25332.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585107091|gb|EWM25332.1|0.000e+0100.00TRAM, LAG1 and CLN8 lipid-sensing domain containin... [more]
gi|553182739|ref|XP_005853778.1|0.000e+098.81hypothetical protein NGA_0438500 [Nannochloropsis ... [more]
gi|553187328|ref|XP_005854585.1|0.000e+098.41hypothetical protein NGA_0175400 [Nannochloropsis ... [more]
gi|553183464|ref|XP_005853901.1|6.079e-4436.21hypothetical protein NGA_0463400 [Nannochloropsis ... [more]
gi|1026765301|gb|OAE26028.1|1.150e-4141.05hypothetical protein AXG93_4601s1270 [Marchantia p... [more]
gi|255071719|ref|XP_002499534.1|1.385e-4139.38predicted protein [Micromonas commoda] >gi|2265147... [more]
gi|308805829|ref|XP_003080226.1|1.756e-4038.86unnamed protein product [Ostreococcus tauri] >gi|1... [more]
gi|1021527767|ref|XP_016207416.1|8.057e-3938.50transmembrane protein 136 [Arachis ipaensis][more]
gi|1012186732|ref|XP_015969640.1|3.034e-3838.05transmembrane protein 136 isoform X2 [Arachis dura... [more]
gi|168015700|ref|XP_001760388.1|4.051e-3840.36predicted protein [Physcomitrella patens] >gi|1626... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna5347rna5347Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications