EWM25433.1, cds5286 (CDS) Nannochloropsis gaditana

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|585107194|gb|EWM25433.1| (cholinesterase precursor [Nannochloropsis gaditana])

HSP 1 Score: 1275 bits (3298), Expect = 0.000e+0
Identity = 627/627 (100.00%), Postives = 627/627 (100.00%), Query Frame = 0

Query:    1 MHRPPKSMRPPAAFLLPVLLHACLNIPHVTADDIITIRVPTMGLEIQGLSSKCGSDQFWGIPYAQRPVERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQLCDGVVVGSEDCLLLNVFRPASPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTLPGNGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACVALPLQEARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHYWAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQPQECNPGSEELGCYHGSEMPYLWAKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSGGASPSRLELREAEGLGTAVVRRASEGPSLGSHGVLREDVEWPAYDDEGGGALVLQLDAGNVTAVPRDLRKQCAFWDSIDVYSK 627
            MHRPPKSMRPPAAFLLPVLLHACLNIPHVTADDIITIRVPTMGLEIQGLSSKCGSDQFWGIPYAQRPVERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQLCDGVVVGSEDCLLLNVFRPASPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTLPGNGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACVALPLQEARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHYWAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQPQECNPGSEELGCYHGSEMPYLWAKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSGGASPSRLELREAEGLGTAVVRRASEGPSLGSHGVLREDVEWPAYDDEGGGALVLQLDAGNVTAVPRDLRKQCAFWDSIDVYSK
Sbjct:    1 MHRPPKSMRPPAAFLLPVLLHACLNIPHVTADDIITIRVPTMGLEIQGLSSKCGSDQFWGIPYAQRPVERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQLCDGVVVGSEDCLLLNVFRPASPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTLPGNGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACVALPLQEARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHYWAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQPQECNPGSEELGCYHGSEMPYLWAKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSGGASPSRLELREAEGLGTAVVRRASEGPSLGSHGVLREDVEWPAYDDEGGGALVLQLDAGNVTAVPRDLRKQCAFWDSIDVYSK 627          

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|553188072|ref|XP_005854731.1| (butyrylcholinesterase precursor, partial [Nannochloropsis gaditana CCMP526] >gi|553196584|ref|XP_005856377.1| butyrylcholinesterase precursor, partial [Nannochloropsis gaditana CCMP526] >gi|422292678|gb|EKU19980.1| butyrylcholinesterase precursor, partial [Nannochloropsis gaditana CCMP526] >gi|422294328|gb|EKU21628.1| butyrylcholinesterase precursor, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 457.988 bits (1177), Expect = 1.409e-156
Identity = 231/231 (100.00%), Postives = 231/231 (100.00%), Query Frame = 0

Query:  171 RLGALGFLTDPTLPGNGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACVALPLQEARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHYWAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLS 401
            RLGALGFLTDPTLPGNGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACVALPLQEARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHYWAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLS
Sbjct:    1 RLGALGFLTDPTLPGNGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACVALPLQEARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHYWAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLS 231          

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|759687520|ref|WP_043404285.1| (carboxylesterase family protein [Archangium violaceum] >gi|667800103|gb|KFA89773.1| hypothetical protein Q664_33400 [Archangium violaceum Cb vi76])

HSP 1 Score: 213.386 bits (542), Expect = 5.935e-58
Identity = 186/607 (30.64%), Postives = 269/607 (44.32%), Query Frame = 0

Query:   46 IQGLSSKCGSDQFWGIPYAQRPV--ERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQL----CDGVVVGSEDCLLLNVFRPA-SPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTLPG------NGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACV--ALPLQE---------ARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHY--WAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQPQ-ECNPGSEELGCYHGSEMPYLWAKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSGGASPSRLELREAEGLGTAVVRRASEGPSLGSHGVLREDVEWPAYDDEGGGALVLQLDAG---NVTAVPRDLRKQCAFWDSI 622
            ++GL+ + G+  F GIP+AQ P    RW+  +P  AE  + P   + F         RCPQ+       V   SEDCL LNV+ PA  P  P PVMV+IHGG FT G     L DGS+LA   G+V+VT+NYRLGALGFL  P L        +GN G++DQ+LALEWVRDNI  FGGD   VTL G+SAGA SACL M++P +  +   L+ ESG C     PL++         A  R   FA  +GC                  +G + ACL   + E L+  S      +     + P +DG++ +PA+P  LL           + + ++LG NRDEG++F     +      +AA    V   A               E LLR YP +         + ++    + CP+  LA                        R    + +P  AY++T++  +P      G  +LG YH +E+P+++   GL+       +  E  ++R +  YW  F   G  +         EG                        V+WPAY  +G   L + ++ G   N+ A      ++C   DS+
Sbjct:   42 VRGLAYEDGTATFLGIPFAQPPTGERRWRAPEP--AEAWSTPREASHFGV-------RCPQVDMRNGKPVEATSEDCLTLNVWTPALQPQKPAPVMVYIHGGGFTLGSTQDELYDGSALA-RAGVVVVTMNYRLGALGFLAHPALGAGAPEGSSGNYGLMDQRLALEWVRDNISAFGGDPTQVTLFGESAGAISACLQMVSPAAHGLFHRLIAESGTCYLGTTPLKDPGTPGQEDSAEERGMRFAREVGC-----------------TEGDIAACLRTRTPEQLLAVSGAALDLIKPRVGFGPVVDGNV-IPAAPRQLLAE------GKYAKVPLMLGTNRDEGTIFTLQAKIDTPEQYEAAVNVRVPGKA---------------EPLLRAYPAKDFTSPKAAFSRLLGDGMFVCPTRRLA------------------------RTLTEQGQPVHAYFFTYEPRKPLGSVFGGMLKLGAYHSAELPFVF---GLSRGRFALDSDAERELSRKLIGYWTRFATQGNPN--------GEGA-----------------------VQWPAYTRDGEQYLEINMELGQGSNLNA------QRCDVLDSL 535          

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|1113031266|gb|OJT25287.1| (hypothetical protein BO221_10355 [Archangium sp. Cb G35])

HSP 1 Score: 209.534 bits (532), Expect = 9.246e-57
Identity = 186/607 (30.64%), Postives = 271/607 (44.65%), Query Frame = 0

Query:   46 IQGLSSKCGSDQFWGIPYAQRPV--ERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQ--LCDGVVV--GSEDCLLLNVFRPA-SPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTLPG------NGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACV--ALPLQE---------ARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACL-TRLSLEVLIPESSERGREMYHY-WAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQPQ-ECNPGSEELGCYHGSEMPYLWAKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSGGASPSRLELREAEGLGTAVVRRASEGPSLGSHGVLREDVEWPAYDDEGGGALVLQLDAG---NVTAVPRDLRKQCAFWDSI 622
            ++GL+ + G   F GIP+AQ P    RW+  +P  AE  + P   + F         RCPQ  + +G  V   SEDCL LNV+ PA  P  P PVMV+IHGG FT G     L DGS+LA   G+V+V++NYRLGALGFL  P L        +GN G++DQ+LALEWVRDNI  FGGD   VTL G+SAGA SACL M++P ++ +   L+ ESG C     PL++         A  R   FA  +GC                  +G + ACL TR   ++L    +          + P +DG++ +P +P  LL           + + ++LG NRDEG++F     +     T   Y + V   A             + E LLR YP +         + ++    + CP+  LA                        R    + +P  AY++T++  +P      G  +LG YH +E+P+++   GL+       +  E  ++R +  YW  F   G  +         EG                        V+WPAY  +G   L + L+ G   N+ A      ++C   DS+
Sbjct:    9 VRGLAYEDGISTFLGIPFAQPPTGERRWRPPEP--AEAWSTPREASHFGV-------RCPQVDMRNGKPVEPASEDCLTLNVWTPALQPQKPAPVMVYIHGGGFTLGSTQDELYDGSALA-RAGVVVVSMNYRLGALGFLAHPALGAVAPEGSSGNYGLMDQRLALEWVRDNISAFGGDPTQVTLFGESAGAISACLQMVSPAAQGLFHRLIAESGTCYLGTTPLKDPGTPELEDSAEERGVRFAREVGC-----------------TEGDIAACLRTRTPGQLLAASGAALDLVKPRVGFGPVVDGNV-IPTAPRQLLAE------GKYAKVPLMLGTNRDEGTIFTLQAKI----DTPEQYEAAVNLRAP-----------GKAEPLLRAYPAKDFSSPKAAFSRLLGDAIFVCPTRRLA------------------------RTLTEQGQPVHAYFFTYEPRKPLGSVFGGMLKLGAYHSAELPFVF---GLSQGRFALDSDAERELSRKIIGYWTRFATQGNPN--------GEGA-----------------------VQWPAYTRDGEQYLEINLELGQGSNLNA------QRCDVLDSL 502          

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|1180161545|ref|WP_083680928.1| (hypothetical protein [Archangium sp. Cb G35])

HSP 1 Score: 209.92 bits (533), Expect = 1.405e-56
Identity = 186/607 (30.64%), Postives = 271/607 (44.65%), Query Frame = 0

Query:   46 IQGLSSKCGSDQFWGIPYAQRPV--ERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQ--LCDGVVV--GSEDCLLLNVFRPA-SPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTLPG------NGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACV--ALPLQE---------ARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACL-TRLSLEVLIPESSERGREMYHY-WAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQPQ-ECNPGSEELGCYHGSEMPYLWAKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSGGASPSRLELREAEGLGTAVVRRASEGPSLGSHGVLREDVEWPAYDDEGGGALVLQLDAG---NVTAVPRDLRKQCAFWDSI 622
            ++GL+ + G   F GIP+AQ P    RW+  +P  AE  + P   + F         RCPQ  + +G  V   SEDCL LNV+ PA  P  P PVMV+IHGG FT G     L DGS+LA   G+V+V++NYRLGALGFL  P L        +GN G++DQ+LALEWVRDNI  FGGD   VTL G+SAGA SACL M++P ++ +   L+ ESG C     PL++         A  R   FA  +GC                  +G + ACL TR   ++L    +          + P +DG++ +P +P  LL           + + ++LG NRDEG++F     +     T   Y + V   A             + E LLR YP +         + ++    + CP+  LA                        R    + +P  AY++T++  +P      G  +LG YH +E+P+++   GL+       +  E  ++R +  YW  F   G  +         EG                        V+WPAY  +G   L + L+ G   N+ A      ++C   DS+
Sbjct:   42 VRGLAYEDGISTFLGIPFAQPPTGERRWRPPEP--AEAWSTPREASHFGV-------RCPQVDMRNGKPVEPASEDCLTLNVWTPALQPQKPAPVMVYIHGGGFTLGSTQDELYDGSALA-RAGVVVVSMNYRLGALGFLAHPALGAVAPEGSSGNYGLMDQRLALEWVRDNISAFGGDPTQVTLFGESAGAISACLQMVSPAAQGLFHRLIAESGTCYLGTTPLKDPGTPELEDSAEERGVRFAREVGC-----------------TEGDIAACLRTRTPGQLLAASGAALDLVKPRVGFGPVVDGNV-IPTAPRQLLAE------GKYAKVPLMLGTNRDEGTIFTLQAKI----DTPEQYEAAVNLRAP-----------GKAEPLLRAYPAKDFSSPKAAFSRLLGDAIFVCPTRRLA------------------------RTLTEQGQPVHAYFFTYEPRKPLGSVFGGMLKLGAYHSAELPFVF---GLSQGRFALDSDAERELSRKIIGYWTRFATQGNPN--------GEGA-----------------------VQWPAYTRDGEQYLEINLELGQGSNLNA------QRCDVLDSL 535          

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|1110673064|ref|WP_071901618.1| (carboxylesterase family protein [Cystobacter ferrugineus] >gi|1109186349|gb|OJH37283.1| hypothetical protein BON30_28675 [Cystobacter ferrugineus])

HSP 1 Score: 204.142 bits (518), Expect = 1.810e-54
Identity = 181/580 (31.21%), Postives = 266/580 (45.86%), Query Frame = 0

Query:   46 IQGLSSKCGSDQFWGIPYAQRPVE--RWKRGQPS--WAEPSTMPEGFAQFDASGH-MPQERCPQ--LCDGVVV--GSEDCLLLNVFRPA-SPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTL------PGNGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACV--ALPLQEARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHY--WAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQP-QECNPGSEELGCYHGSEMPYLWAKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSGGASPSRLELREAEGLGTAVVRRASEGPSLGSHGVLREDVEWPAYDDEGGGALVLQLDAGN 604
            ++GL+ + GS  F GIP+AQ P    RW+  +P+  W+ P          DAS   +P   CPQ  L +G  +   SEDCL LNV+ PA  P  P PVMV+IHGG+FT G     L DGS+LA   G+V+V++NYRLGALGFL  P L        +GN G++DQ+LALEWVRD++  FGGD   VTL G+SAGA SACL ML+P ++ +   ++ ESG C     PL++  T  +  +A          R        G  +G + ACL   + E L+  S      +     + P +DG++ +PA+P  LL           + + ++LG NRDEG++F     +     T   Y + V   A               E LLR YP +         + ++    + CP+  LA                  V+Q          +P  AY++T++  +       G  +LG YH +E+P+++   GL+       +  E  ++  +  YW  F   G  +        AEG                        VEWPAY  +G   L + L+ G+
Sbjct:   42 VRGLAYEDGSAAFLGIPFAQPPTGELRWRAPEPAAAWSTPR---------DASHFGLP---CPQADLRNGKPIEGTSEDCLTLNVWTPALEPQKPAPVMVYIHGGAFTLGSSRDELYDGSALA-HAGVVVVSMNYRLGALGFLAHPALGAGAPAESSGNYGLMDQRLALEWVRDHVSAFGGDPTNVTLFGESAGAISACLQMLSPAAQGLFHRVIAESGTCYLGTTPLKDPGTPEQEDSAE--------ERGVRFAREVGCTEGDIAACLRTRTPEQLLAASGAALDLIKPRVGFGPVVDGNV-IPAAPRQLLAEGKY------AKVPLMLGANRDEGTIFTLQAKID----TPEQYEAAVNVRAP-----------GNAEPLLRAYPAKDFASPKAAFSRLLGDAMFVCPTRRLARTL---------------VEQG---------QPVHAYFFTYEPKKAFGSIFGGFLKLGAYHSAELPFVF---GLSRGRFALDSDAERELSTKLIGYWTRFATGGNPN--------AEGA-----------------------VEWPAYTRDGEKYLEINLELGH 520          

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|1175165890|ref|WP_081713811.1| (hypothetical protein [Cystobacter fuscus])

HSP 1 Score: 199.134 bits (505), Expect = 2.179e-52
Identity = 164/522 (31.42%), Postives = 243/522 (46.55%), Query Frame = 0

Query:   46 IQGLSSKCGSDQFWGIPYAQRPV--ERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQLCDGVVVGSEDCLLLNVFRPA-SPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTL------PGNGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGAC--VALPLQE---------ARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHY--WAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQPQECNPGSE-ELGCYHGSEMPYLWAKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSG 544
            +QGL+   G   F GIP+AQ P    RW+  +P+ A   + P   ++F +       R  +  +G    SEDCL LNV+ PA  P  P PVMV+IHGG+FT G     L DGS+LA   G+V+V++NYRLGALGFL  P L        +GN G++DQ+LALEWVRD++  FGGD   VTL G+SAGA SACL M++P ++ +   ++ ESG C     PL++         A  R   FA  +GC                  +G + ACL   + E L+  S      +     + P +DG++ +PA+P  LL           + + +LLG NRDEG++F     +      +AA    V   A               E LLR YP +         + ++    + CP+  LA                  V+Q          +P  AY++T++  +      G   +LG YH +E+P+++   GL+       +  E  ++  +  YW  F   G
Sbjct:   71 VQGLAYADGGAAFLGIPFAQPPTGERRWRAPEPAAA--WSTPRDASRFGSPCPQADMRNGKPIEGT---SEDCLTLNVWTPALEPQKPAPVMVYIHGGAFTLGSSRDELYDGSALA-HAGVVVVSMNYRLGALGFLAHPALGAGAPAESSGNYGLMDQRLALEWVRDHVSAFGGDPTNVTLFGESAGAISACLQMVSPAAQGLFHRVIAESGTCYLTNTPLKDPGTPEQEDSAEERGVRFAREVGC-----------------TEGDIAACLRTRTPEQLLAASGAALDLLKPRVGFGPVVDGNV-IPAAPRQLLAE------GKYARVPLLLGSNRDEGTIFTLQAKIDTPEQYEAAVNVRVPGKA---------------EPLLRAYPAKDFTSPKAAFSRLLGDAMFVCPTRQLARTL---------------VEQG---------QPVHAYFFTYEPKKAFGSIFGRFLKLGAYHSAELPFVF---GLSRGRFALDSDSERELSTKLIGYWTRFATGG 520          

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|528055537|gb|EPX60330.1| (putative carboxylesterase [Cystobacter fuscus DSM 2262])

HSP 1 Score: 198.364 bits (503), Expect = 2.593e-52
Identity = 164/522 (31.42%), Postives = 243/522 (46.55%), Query Frame = 0

Query:   46 IQGLSSKCGSDQFWGIPYAQRPV--ERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQLCDGVVVGSEDCLLLNVFRPA-SPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTL------PGNGNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGAC--VALPLQE---------ARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHY--WAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQPQECNPGSE-ELGCYHGSEMPYLWAKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSG 544
            +QGL+   G   F GIP+AQ P    RW+  +P+ A   + P   ++F +       R  +  +G    SEDCL LNV+ PA  P  P PVMV+IHGG+FT G     L DGS+LA   G+V+V++NYRLGALGFL  P L        +GN G++DQ+LALEWVRD++  FGGD   VTL G+SAGA SACL M++P ++ +   ++ ESG C     PL++         A  R   FA  +GC                  +G + ACL   + E L+  S      +     + P +DG++ +PA+P  LL           + + +LLG NRDEG++F     +      +AA    V   A               E LLR YP +         + ++    + CP+  LA                  V+Q          +P  AY++T++  +      G   +LG YH +E+P+++   GL+       +  E  ++  +  YW  F   G
Sbjct:   48 VQGLAYADGGAAFLGIPFAQPPTGERRWRAPEPAAA--WSTPRDASRFGSPCPQADMRNGKPIEGT---SEDCLTLNVWTPALEPQKPAPVMVYIHGGAFTLGSSRDELYDGSALA-HAGVVVVSMNYRLGALGFLAHPALGAGAPAESSGNYGLMDQRLALEWVRDHVSAFGGDPTNVTLFGESAGAISACLQMVSPAAQGLFHRVIAESGTCYLTNTPLKDPGTPEQEDSAEERGVRFAREVGC-----------------TEGDIAACLRTRTPEQLLAASGAALDLLKPRVGFGPVVDGNV-IPAAPRQLLAE------GKYARVPLLLGSNRDEGTIFTLQAKIDTPEQYEAAVNVRVPGKA---------------EPLLRAYPAKDFTSPKAAFSRLLGDAMFVCPTRQLARTL---------------VEQG---------QPVHAYFFTYEPKKAFGSIFGRFLKLGAYHSAELPFVF---GLSRGRFALDSDSERELSTKLIGYWTRFATGG 497          

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|499191710|ref|WP_010889250.1| (carboxylesterase/lipase family protein [Deinococcus radiodurans] >gi|15807606|ref|NP_296345.1| carboxylesterase, type B [Deinococcus radiodurans R1] >gi|6460456|gb|AAF12163.1|AE002092_1 carboxylesterase, type B [Deinococcus radiodurans R1] >gi|1026245144|gb|ANC70357.1| carboxylesterase [Deinococcus radiodurans R1])

HSP 1 Score: 192.2 bits (487), Expect = 4.162e-50
Identity = 170/510 (33.33%), Postives = 232/510 (45.49%), Query Frame = 0

Query:   52 KCGSDQFWGIPYAQRPVERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQLC-----DGVVVGSEDCLLLNVFRPASPGSPMPVMVFIHGGSFTEG-GIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTLPGN----GNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACVALPLQEARTRT----ETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHYWAPALDGDLFLPASPATLLDPSVTPPLAPASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNAENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQPQECNP--GSEELGCYHGSEMPYLWAKPGLTPCGDGETNGPETA-MARAMRSYWGAFVRSG 544
            + G   + GIPYAQ PV     G   W  P  +P   +Q + +   P   C Q       D  V G+EDCL LNV+ P +     PVMV+IHGGSF  G G D    D S LA E+G+V V++NYRLGALGFL  P L       GNLG+LDQQLAL+WVRDNI  FGGDA+ VT+ G+SAG  S C  + +P +  +    +++SG C A  + ++R         FAA +GC P              AD     ACL  +  + L+   S           P + GD  +P +PA +L          A PL  L+G N +EG++F A     G    D   A ++   A+  G   P          L  Y  R  G   +  AA      +ACPS  LA       +D S    V+          + E R P+         +P +  P  G    G +HGSE+  +   P  +  G+     P  A ++R M++YW  F R+G
Sbjct:   58 RAGVRSWLGIPYAQPPV-----GDARWQPPRALPA--SQAERTTTQPGAACVQTLSVPGMDKQVRGAEDCLFLNVYAPTN-AQKAPVMVWIHGGSFQMGAGSD---YDLSVLAREQGVVAVSLNYRLGALGFLATPALDTAQGTAGNLGLLDQQLALKWVRDNIAAFGGDAQNVTVFGESAGGMSICAQLASPGAAGLFDKAIIQSGPCTAPGIMQSRAEAYNLGSRFAAAVGCDP--------------AD----AACLRAVPADKLVLTRSPNAAFPGAVPFPPVYGDSTVPRAPAEVLRQGQN-----AVPL--LIGTNLNEGTLFAA---FVGDPRRDLNAAEFLGLNAVLNGPNAP--------RALLAYNSRTSGTRTQAAAASATDSLFACPSSNLA-------RDISRFTPVY----------SYEFRDPNP-------PRPAQLRPTVGVPSFGAFHGSEIVSVTGTP--SGLGNPSDFTPAQAELSRTMQTYWANFARTG 494          

BLAST of EWM25433.1 vs. NCBI_GenBank
Match: gi|1093730948|ref|WP_070948831.1| (carboxylesterase family protein [Mycobacterium chelonae] >gi|1092095602|gb|OHU39015.1| carboxylesterase [Mycobacterium chelonae])

HSP 1 Score: 191.43 bits (485), Expect = 4.287e-50
Identity = 169/503 (33.60%), Postives = 229/503 (45.53%), Query Frame = 0

Query:   58 FWGIPYAQRPVERWKRGQPSWAEPSTMPEGFAQFDASGHMPQERCPQL-CDGVVVGSEDCLLLNVFRPASPGSPMPVMVFIHGGSFTEGGIDSPLLDGSSLAAEEGIVIVTINYRLGALGFLTDPTLPGN-GNLGMLDQQLALEWVRDNIGFFGGDAEAVTLAGQSAGAFSACLHMLAPKSRAMLQALVMESGACVA-LPLQEARTRTETFAAGLGCRPGPGRRSPSNGSSEGADDGGLVACLTRLSLEVLIPESSERGREMYHYWAPALDGDLFLPASPATLLDPSVTPPLAP-----ASPLNVLLGCNRDEGSVFVASTGLGGAGGTDAAYASWVRTGAMEYGQGLPPLSLAQREELLRVYPPRGDGKNA-ENVAAIIAHVRYACPSLFLASHYGGGRQDASEGVSVWHVQQAGRRRHNIEMRPPSAYYYTFQYVQP-----QECNPGSEELGCYHGSEMPYLW--AKPGLTPCGDGETNGPETAMARAMRSYWGAFVRSG 544
            F  IPYA  PV   +  +P   EP +        DA+G  P   CPQ   +  +  SEDCL+LNV  P      +PV+V+IHGG+F  G  +    +   LAA+ GI +VTINYRLG LGFL  P L    GN G+LDQ+ AL WVRDNI  FGGD   VT+ GQSAG  S C  M+APK+  + ++ +MES  C A  P+  A   +  +AA +GC  GP                G  ACL  LS++ L        R+     +P   G + LP SP T       PPL       +SP+ VL+G N +E +VF A             Y      GA EY + L      +  EL R YP    G N    +AAI     YACP+L +A                    +A  R+  +         Y +++  P     Q+       LG  HGSE+ YL+  ++P          +    A++  M +YW  FV++G
Sbjct:   47 FTAIPYAAPPVGPRRFTEPEPVEPWSDTR-----DATG--PGVECPQPGAESDLTQSEDCLVLNVTMPRHTQGKVPVLVWIHGGAFIAG--NGVGYNARKLAAQGGIAVVTINYRLGTLGFLAAPGLSDVIGNYGLLDQEAALRWVRDNIAAFGGDPGQVTIGGQSAGGVSVCDLMVAPKASGLFRSAIMESAPCQAQAPVSSAVRDSTAYAADVGCPAGP----------------GFAACLRALSVDAL--------RD-----SPQFTG-IGLPVSPVTGTPELPAPPLDAFTSGLSSPVPVLIGSNANEATVFEAQQ-----------YQHKPVPGAEEYQRALESKYADRATELGRRYPLSAYGGNVLAALAAIDTDNSYACPTLDMA--------------------EALVRKAPV---------YAYEFADPAAPVEQQYQGAPLPLGASHGSELGYLFDLSRP---------LSQESAALSTQMITYWAQFVKTG 461