EWM25526.1, cds5145 (CDS) Nannochloropsis gaditana

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|585107292|gb|EWM25526.1| (kazal-type serine protease inhibitor domain protein [Nannochloropsis gaditana])

HSP 1 Score: 1149.81 bits (2973), Expect = 0.000e+0
Identity = 570/570 (100.00%), Postives = 570/570 (100.00%), Query Frame = 0

Query:    1 MKYFVQIFCGKMRTLSLIATAAGLVGVAYGAEQTCGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETECPLVSVQVTSPAMHRYRGRVGGVMVMMTVFGILLGALAIF 570
            MKYFVQIFCGKMRTLSLIATAAGLVGVAYGAEQTCGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETECPLVSVQVTSPAMHRYRGRVGGVMVMMTVFGILLGALAIF
Sbjct:    1 MKYFVQIFCGKMRTLSLIATAAGLVGVAYGAEQTCGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETECPLVSVQVTSPAMHRYRGRVGGVMVMMTVFGILLGALAIF 570          

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|1005209767|gb|KYF80270.1| (hypothetical protein BE17_25790 [Sorangium cellulosum])

HSP 1 Score: 156.377 bits (394), Expect = 5.801e-39
Identity = 132/397 (33.25%), Postives = 162/397 (40.81%), Query Frame = 0

Query:   35 CGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECP 430
            CG  L   C  G FC   L      + G   + G C+  P  C +  +PVCGCDG  Y NECEAN  GVS+ S G C                                  CGG   + C     C +    +CG A+ +G C PT +  C  +Y PVCGCDG TY NAC A    GV V   G CE   E    +  + GG + E                      P   C               E  C + +    G CA  P  C     T  +  VCGC+G TY  EC A  AG+S+  RG C     PAP   CGG   +A    + G +C Y    +C   D  GTC   P  CT   DPVCGC+ +TY NAC A  +G S+   GECP
Sbjct:   58 CGGLLGLSCKRGFFCDYEL----EAMCGAFDQTGTCERIPQACTREYNPVCGCDGKTYGNECEANSAGVSISSVGECAP---------------------------PPEQICGGIAGLACDRGSFCDFAPDAQCGAADQTGVCVPTPE-ACTEEYNPVCGCDGKTYGNACAAN-SAGVSVASRGECEPPPE---QLCGSRGGVECE----------------------PGFFCN-----------FAPEAACGTFD--APGTCAPIPEAC-----TFEYNPVCGCNGETYGNECAANAAGVSVASRGEC----APAPGKICGGLAGFA---CEPGSFCNYSLDAICGAADQTGTCEVIPEACTANYDPVCGCDDRTYSNACAAHQSGVSVALLGECP 371          

HSP 2 Score: 140.584 bits (353), Expect = 3.000e-33
Identity = 127/395 (32.15%), Postives = 162/395 (41.01%), Query Frame = 0

Query:  140 GTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAA-GVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETECP 532
            G   + CGG   + C     C YE    CG  + +G CE      C  +Y PVCGCDG TY N C A    GV +   G C                     P   +  G   +  +R  G F       D  CG                    GVC   P  C     T  +  VCGCDG TY   C A  AG+S+  RG C     P P   CG +G       + G++C +     C   D+ GTC   P  CT E +PVCGCNG+TY N C A  AG S+ S+GEC                   A   G  C   AG  C  G +C Y+   +CG+ A   G C V PE+C    ++   VCGCD  +++ AC A  +GV++    ECP
Sbjct:   52 GHEGSACGGLLGLSCKRGFFCDYELEAMCGAFDQTGTCERIPQ-ACTREYNPVCGCDGKTYGNECEAN-SAGVSISSVGECA-------------------PPPEQICGGIAGLACDR--GSFCDF--APDAQCGAAD---------------QTGVCVPTPEAC-----TEEYNPVCGCDGKTYGNACAANSAGVSVASRGEC----EPPPEQLCGSRGGVE---CEPGFFCNFAPEAACGTFDAPGTCAPIPEACTFEYNPVCGCNGETYGNECAANAAGVSVASRGEC-------------------APAPGKICGGLAGFACEPGSFCNYSLDAICGA-ADQTGTCEVIPEACT---ANYDPVCGCDDRTYSNACAAHQSGVSVALLGECP 371          

HSP 3 Score: 90.5077 bits (223), Expect = 6.262e-16
Identity = 76/217 (35.02%), Postives = 93/217 (42.86%), Query Frame = 0

Query:   10 GKMRTLSLIATAAGLVGVAYGA-----EQTCGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNC-PKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTC 220
            GK    +  A +AG+   + G      EQ CG+R   EC  G FC+           G     G C   P+ C    +PVCGC+G  Y NEC AN  GVSV S+G C P  G +                            CGG     C P   C Y     CG A+ +G CE   +  C  +Y PVCGCD  TYSNAC A +Q+GV V   G C
Sbjct:  188 GKTYGNACAANSAGVSVASRGECEPPPEQLCGSRGGVECEPGFFCNFA----PEAACGTFDAPGTCAPIPEACTFEYNPVCGCNGETYGNECAANAAGVSVASRGECAPAPGKI----------------------------CGGLAGFACEPGSFCNYSLDAICGAADQTGTCEVIPE-ACTANYDPVCGCDDRTYSNACAA-HQSGVSVALLGEC 370          

HSP 4 Score: 87.0409 bits (214), Expect = 7.203e-15
Identity = 94/316 (29.75%), Postives = 114/316 (36.08%), Query Frame = 0

Query:   32 EQTCGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNC-PKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQ 346
            EQ CG      C  GSFC            G   + G+C   P+ C +  +PVCGCDG  Y N C AN  GVSV S+G C P    L                            CG    ++C P   C +     CG  +  G C P  +  C  +Y PVCGC+G TY N C A    GV V   G C                     PA   I G    G   E G F  +  + D  CG                    G C   P  C     T  +  VCGCD  TY   C A Q+G+S+   G C R+
Sbjct:  135 EQICGGIAGLACDRGSFCDFA----PDAQCGAADQTGVCVPTPEACTEEYNPVCGCDGKTYGNACAANSAGVSVASRGECEPPPEQL----------------------------CGSRGGVECEPGFFCNFAPEAACGTFDAPGTCAPIPE-ACTFEYNPVCGCNGETYGNECAANAA-GVSVASRGEC--------------------APAPGKICGG-LAGFACEPGSFCNY--SLDAICGAAD---------------QTGTCEVIPEAC-----TANYDPVCGCDDRTYSNACAAHQSGVSVALLGECPRR 373          

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|1005211444|gb|KYF81462.1| (hypothetical protein BE11_36605 [Sorangium cellulosum])

HSP 1 Score: 148.673 bits (374), Expect = 4.638e-36
Identity = 131/392 (33.42%), Postives = 163/392 (41.58%), Query Frame = 0

Query:   39 LAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGEC 429
            L   C  G FC   L      + G   + G C+  P++C +  +PVCGCDG  Y NECEAN  GVSV ++G C                                 +CGG   + C     C +     CG A+  G C PT +  C  ++ PVCGCDG TY NAC+A    GV V   G C  A  G A   D  G +   E A  L               FP        +CG                    G CA  P  C     T  +  VCGCDG TY   C A  AG+S+   G C     PAP   CG  GL    C+  G +C Y    +C   D  G C   P  CT+E +PVCGC+ +TY NAC A  AG ++ S+GEC
Sbjct:   60 LGLSCRSGFFCDYEL----DAMCGAFDQTGTCERIPEVCTREYNPVCGCDGETYGNECEANAAGVSVSARGECEP---------------------------PPRQECGGLLGLACDEGSFCDFAPDAMCGAADQMGVCVPTPE-GCTKEFAPVCGCDGNTYGNACMANAA-GVSVASRGECAPAPGGGA--GDACGTRGAAECAPGLFCD------------FPPR-----AECGSFDL---------------PGACAPIPEAC-----TFQYDPVCGCDGKTYGNACAANSAGVSVASSGEC----APAPEKICG--GLLGLECDA-GTFCNYSLEAMCGAADMTGVCDPIPEACTEEYNPVCGCDDRTYGNACAANSAGVAVASRGEC 372          

HSP 2 Score: 134.806 bits (338), Expect = 3.198e-31
Identity = 127/386 (32.90%), Postives = 159/386 (41.19%), Query Frame = 0

Query:  152 IKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQ-EGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPA----DSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETEC 531
            + C     C YE    CG  + +G CE   ++ C  +Y PVCGCDG TY N C A    GV V   G CE                                  +  GGL           C    +C    +  C + + +  GVC   P  C     T  F  VCGCDG TY   C A  AG+S+  RG C     PAP     D+CG +G  A  C   G +C +  R  C   D  G C   P  CT + DPVCGC+GKTY NAC A  AG S+ S GEC             +  GLL            GL C  G +C Y+       A   G+C   PE+C  +    + VCGCD  ++  AC A  AGVA+ +  EC
Sbjct:   62 LSCRSGFFCDYELDAMCGAFDQTGTCERIPEV-CTREYNPVCGCDGETYGNECEANAA-GVSVSARGECEPPPR------------------------------QECGGLLGLA-------CDEGSFCDFAPDAMCGAADQM--GVCVPTPEGC-----TKEFAPVCGCDGNTYGNACMANAAGVSVASRGEC----APAPGGGAGDACGTRG--AAEC-APGLFCDFPPRAECGSFDLPGACAPIPEACTFQYDPVCGCDGKTYGNACAANSAGVSVASSGECAPAPEK-------ICGGLL------------GLECDAGTFCNYSLEAMCGAADMTGVCDPIPEACTEE---YNPVCGCDDRTYGNACAANSAGVAVASRGEC 372          

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|1101191513|gb|OIP42875.1| (hypothetical protein AUK47_03540 [Deltaproteobacteria bacterium CG2_30_63_29])

HSP 1 Score: 151.369 bits (381), Expect = 2.133e-35
Identity = 153/498 (30.72%), Postives = 196/498 (39.36%), Query Frame = 0

Query:   35 CGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGL-HGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETEC 531
            CG  L   CP G +C+   GE   + GG     G+C   P+ C     PVCGCD   Y N C A   GV+V S G C                            G++T  CGG + I+CA   VCI+     CG A+ +G C    +  C  +Y PVCGCDG TY N C A                    TA  A  S   + E P N             E G F    C A + C      C + +G          GVC  +P  C        +  VCGCDG TY   C A  AG++I + G C          +CGG   +       G  C+   G C++ D++G C   P  CT+E  PVCGC+G TY N C A  AG S+   GEC    +                     C   A + C  G  C +  G C +     G C  QP++ V  P     VC CDG ++   CDA  AG A+  E  C
Sbjct:  119 CGGALDVLCPAGQYCALASGE---VCGG----SGVCTATPEACTMEYAPVCGCDNNTYGNGCSAAAAGVNVASAGEC----------------------------GSTTVACGGRSNIQCAAGEVCIFPE-FTCGAADEAGTCTAVPE-ACTMEYAPVCGCDGVTYGNGCSA--------------------TAAGASISYAGECETPKN-------------ECGGFAGLACAAGELCVYPDNTCDLADG---------MGVCVPQPGACPE-----LWQPVCGCDGQTYGNRCDAIAAGVTIDHVGEC------GAIANCGGIAGFQ---CASGEVCINADGTCNIMDNMGACLVAPEACTEEYAPVCGCDGVTYGNRCAATSAGVSVDHLGECRSTQM---------------------CGGLAAVQCPTGEVCVFGVGTC-AMMDPTGTC--QPKT-VGCPDVWMPVCSCDGVTFGNECDAIAAGAAISHEGAC 498          

HSP 2 Score: 139.043 bits (349), Expect = 3.065e-31
Identity = 123/400 (30.75%), Postives = 160/400 (40.00%), Query Frame = 0

Query:   30 GAEQTCGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGEC 429
            GA   CG     +C  G  C    G C  +        G C + P+ C +   PVCGCDGV Y N C A   GVSV   G C                              ST  CGG  A++C    VC++  G  C   + +G C+P K + C   + PVC CDG T+ N C A    G  +   G CE  +                                  GGL       A+  C     C +  G C + +P   G+C   P+ C       A+  VCGCDG TY + C A  AG +I + GAC   G     +SCGG      TC+     C+Y  G C+  D LGTC  +   C+    PVCGC+  TY N C+A  +G SI + G C
Sbjct:  345 GAIANCGGIAGFQCASGEVCINADGTCNIM-----DNMGACLVAPEACTEEYAPVCGCDGVTYGNRCAATSAGVSVDHLGEC-----------------------------RSTQMCGGLAAVQCPTGEVCVFGVGT-CAMMDPTGTCQP-KTVGCPDVWMPVCSCDGVTFGNECDA-IAAGAAISHEGACETTT--------------------------------GCGGL-------ANIGCATGEICVIAAGTCGAMDP--RGLCEPIPVSCPD-----AYIPVCGCDGVTYSSPCDANVAGAAIDHNGACGSVG-----ESCGG--FVGLTCSSSNAACIYADGSCNGADMLGTCVEQGMTCSMGYSPVCGCDKVTYGNRCEAEQSGVSIDTIGAC 654          

HSP 3 Score: 134.806 bits (338), Expect = 8.449e-30
Identity = 126/391 (32.23%), Postives = 162/391 (41.43%), Query Frame = 0

Query:  141 TSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETEC 531
            T+ T+CGG   + C     C   +G  CG   GSG C  T +  C  +Y PVCGCD  TY N C A    GV V   G C     G+  +A   G  +++  A ++              +FP   C A  +                      G C   P  C     T  +  VCGCDG TY   C A  AG SI Y G C       P + CGG   +A      G  C+Y    C L D +G C  +P  C +   PVCGC+G+TY N C A  AG +I   GEC                G +AN     C   AG  C+ G  C  A G C +     G C+V PE+C  + +    VCGCDG ++   C A  AGV++    EC
Sbjct:  114 TTNTNCGGALDVLCPAGQYCALASGEVCG---GSGVCTATPE-ACTMEYAPVCGCDNNTYGNGCSAAAA-GVNVASAGEC-----GSTTVA-CGGRSNIQCAAGEVC-------------IFPEFTCGAADE---------------------AGTCTAVPEAC-----TMEYAPVCGCDGVTYGNGCSATAAGASISYAGEC-----ETPKNECGG---FAGLACAAGELCVYPDNTCDLADGMGVCVPQPGACPELWQPVCGCDGQTYGNRCDAIAAGVTIDHVGEC----------------GAIAN-----CGGIAGFQCASGEVCINADGTC-NIMDNMGACLVAPEACTEEYA---PVCGCDGVTYGNRCAATSAGVSVDHLGEC 421          

HSP 4 Score: 124.02 bits (310), Expect = 3.020e-26
Identity = 163/571 (28.55%), Postives = 206/571 (36.08%), Query Frame = 0

Query:    1 MKYFVQIFCGKMRTLSLIATAAGLVGVAYGAE-----QTCGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDAS-------------------EGTADIADTSGGQDVEEPA-----------------NDLIDGAEEVGEEREGGLFPTHKC--TADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSE-GYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHS-VCGCDGHSWATACDAAMAGVALK 526
            M+Y     C      +  + AA  V VA   E       CG R   +C  G  C      C     G   + G C   P+ C     PVCGCDGV Y N C A   G S+   G C                             T   +CGG   + CA   +C+Y   N C  A+G G C P     C   + PVCGCDG TY N C A    GV +   G C   +                   +GT +I D  G   V   A                 N     +  V  +  G    T  C   A   C     C    G C+  +P   G C  K + C        +  VC CDG T+  EC A  AG +I + GAC         ++  G G  A      G  C+   G C   D  G C   P  C     PVCGC+G TY + C A +AG++I   G C  V                    G  C    GLTCS     C YA G C + A   G CV Q  +C    S  +S VCGCD  ++   C+A  +GV++ 
Sbjct:  156 MEYAPVCGCDNNTYGNGCSAAAAGVNVASAGECGSTTVACGGRSNIQCAAGEVCIFPEFTC-----GAADEAGTCTAVPEACTMEYAPVCGCDGVTYGNGCSATAAGASISYAGECE----------------------------TPKNECGGFAGLACAAGELCVYP-DNTCDLADGMGVCVPQPG-ACPELWQPVCGCDGQTYGNRCDA-IAAGVTIDHVGECGAIANCGGIAGFQCASGEVCINADGTCNIMDNMGACLVAPEACTEEYAPVCGCDGVTYGNRCAATSAGVSVDHLGECRSTQMCGGLAAVQCPTGEVCVFGVGTCAMMDPT--GTCQPKTVGCPD-----VWMPVCSCDGVTFGNECDAIAAGAAISHEGAC---------ETTTGCGGLANIGCATGEICVIAAGTCGAMDPRGLCEPIPVSCPDAYIPVCGCDGVTYSSPCDANVAGAAIDHNGACGSV--------------------GESCGGFVGLTCSSSNAACIYADGSC-NGADMLGTCVEQGMTC----SMGYSPVCGCDKVTYGNRCEAEQSGVSID 649          

HSP 5 Score: 107.842 bits (268), Expect = 5.918e-21
Identity = 83/242 (34.30%), Postives = 103/242 (42.56%), Query Frame = 0

Query:  294 EGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETECPLVS 535
             GVC   P  C     T  +  VCGCD  TY   C A  AG+++   G C          +CGG+   +      G  C++    C   D  GTCT+ P  CT E  PVCGC+G TY N C A  AG+SI   GEC                          C   AGL C+ G  C Y    C   A   G+CV QP +C   P     VCGCDG ++   CDA  AGV +    EC  ++
Sbjct:  144 SGVCTATPEAC-----TMEYAPVCGCDNNTYGNGCSAAAAGVNVASAGECGSTTV-----ACGGR---SNIQCAAGEVCIFPEFTCGAADEAGTCTAVPEACTMEYAPVCGCDGVTYGNGCSATAAGASISYAGEC--------------------ETPKNECGGFAGLACAAGELCVYPDNTC-DLADGMGVCVPQPGAC---PELWQPVCGCDGQTYGNRCDAIAAGVTIDHVGECGAIA 348          

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|1005324891|ref|WP_061609931.1| (hypothetical protein [Sorangium cellulosum] >gi|1005192906|gb|KYF67313.1| hypothetical protein BE15_42595 [Sorangium cellulosum])

HSP 1 Score: 143.665 bits (361), Expect = 2.249e-34
Identity = 132/392 (33.67%), Postives = 165/392 (42.09%), Query Frame = 0

Query:   39 LAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGEC 429
            L   C  G FC   L      + G   + G C+  P++C +  +PVCGCDG  Y NECEAN  GVSV ++G C                                 +CGG   + C     C +     CG A+ +G C PT +  C  ++ PVCGCDG TYSNAC+A    GV V   G C  A  G   + D  G +   E A  L               FP        DCG                    G CA  P  C     T  +  VCGCDG TY   C A  AG+S+   G C     PAP   CG  GL    C+  G +C Y    +C   D  G C   P  CT+E +PVCGC+ +TY NAC A  AG ++ S+GEC
Sbjct:   62 LGLSCRSGFFCDYEL----EAMCGAFDQTGTCERIPEVCTREYNPVCGCDGKTYGNECEANGAGVSVSARGECAPP---------------------------PRQECGGLLGLACDEGSFCDFAPDAMCGAADQTGVCVPTPE-GCTKEFAPVCGCDGNTYSNACMANAA-GVSVASSGECAPAPGG--GVGDACGTRGAAECAPGLFCD------------FPPR-----ADCGSFDL---------------PGACAPMPEAC-----TFQYDPVCGCDGKTYGNACAANAAGVSVASSGEC----APAPEKICG--GLLGLECDA-GSFCNYSLEAMCGAADMTGVCDPIPEACTEEYNPVCGCDDRTYGNACAANSAGVAVASRGEC 374          

HSP 2 Score: 116.701 bits (291), Expect = 7.814e-25
Identity = 125/386 (32.38%), Postives = 156/386 (40.41%), Query Frame = 0

Query:  152 IKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQ-EGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPA----DSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETEC 531
            + C     C YE    CG  + +G CE   ++ C  +Y PVCGCDG TY N C A    GV V   G C                                   +  GGL           C    +C    +  C + +    GVC   P  C     T  F  VCGCDG TY   C A  AG+S+   G C     PAP     D+CG +G  A  C   G +C +  R  C   D  G C   P  CT + DPVCGC+GKTY NAC A  AG S+ S GEC             +  GLL            GL C  G +C Y+       A   G+C   PE+C  +    + VCGCD  ++  AC A  AGVA+ +  EC
Sbjct:   64 LSCRSGFFCDYELEAMCGAFDQTGTCERIPEV-CTREYNPVCGCDGKTYGNECEAN-GAGVSVSARGECAPPPR------------------------------QECGGLLGLA-------CDEGSFCDFAPDAMCGAAD--QTGVCVPTPEGC-----TKEFAPVCGCDGNTYSNACMANAAGVSVASSGEC----APAPGGGVGDACGTRG--AAEC-APGLFCDFPPRADCGSFDLPGACAPMPEACTFQYDPVCGCDGKTYGNACAANAAGVSVASSGECAPAPEK-------ICGGLL------------GLECDAGSFCNYSLEAMCGAADMTGVCDPIPEACTEE---YNPVCGCDDRTYGNACAANSAGVAVASRGEC 374          

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|769244757|ref|WP_044988273.1| (hypothetical protein [Sorangium cellulosum] >gi|1005246112|gb|KYG05532.1| hypothetical protein BE21_40070 [Sorangium cellulosum])

HSP 1 Score: 141.739 bits (356), Expect = 1.216e-33
Identity = 130/389 (33.42%), Postives = 160/389 (41.13%), Query Frame = 0

Query:   43 CPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECP 430
            C  G FC   L    ++ G  +  G  C+  P +C +  +PVCGCDG  Y NEC AN  GVSV S+G C                                 DCGG   + C P   C Y     CG A+ +G C P  +  C  +Y PVCGCDG TY NAC+A       V  PG C  A  G   +  T G    E                      P   C               E  C + +    G CA  P  C     T  +  VCGCDG TY   C A  AG+S+  RG C     P+    CGG  L    C+  G +C Y    +C   D  G C + P  CT++ DPVCGC+ KTY NAC A  AG S+  +GECP
Sbjct:   62 CKKGYFCDYEL---EAMCGAFDMTG-TCERIPKVCTEEYNPVCGCDGKTYGNECAANSAGVSVASRGECEPP---------------------------PARDCGGLAGLACEPGEFCDYAPDAMCGAADQTGVCAPIPE-GCTKEYNPVCGCDGNTYGNACMAAAAGVS-VASPGECAPAPGG---VCGTRGAAACE----------------------PGFFCD-----------FAPEAACGATD--LPGACAPIPEAC-----TFEYNPVCGCDGNTYGNACAANAAGVSVASRGEC----APSEVQHCGG--LAGLACDP-GEFCNYSLEAICGAADQTGVCETIPEACTEQYDPVCGCDDKTYSNACFAHAAGVSVALRGECP 367          

HSP 2 Score: 116.316 bits (290), Expect = 9.777e-25
Identity = 129/384 (33.59%), Postives = 164/384 (42.71%), Query Frame = 0

Query:  152 IKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYC-HVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYA-AGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETECP 532
            + C   + C YE    CG  + +G CE    + C  +Y PVCGCDG TY N C A    GV V   G CE                    PA D             GGL       A   C    +C +  +  C + +    GVCA  P  C     T  +  VCGCDG TY   C A  AG+S+   G C     PAP   CG +G  A    + G++C +     C   D  G C   P  CT E +PVCGC+G TY NAC A  AG S+ S+GEC    +      GGL                AGL C  G +C Y+   +CG+ A   G+C   PE+C         VCGCD  +++ AC A  AGV++    ECP
Sbjct:   60 LSCKKGYFCDYELEAMCGAFDMTGTCERIPKV-CTEEYNPVCGCDGKTYGNECAAN-SAGVSVASRGECE------------------PPPARDC------------GGL-------AGLACEPGEFCDYAPDAMCGAAD--QTGVCAPIPEGC-----TKEYNPVCGCDGNTYGNACMAAAAGVSVASPGEC----APAPGGVCGTRGAAA---CEPGFFCDFAPEAACGATDLPGACAPIPEACTFEYNPVCGCDGNTYGNACAANAAGVSVASRGECAPSEVQH---CGGL----------------AGLACDPGEFCNYSLEAICGA-ADQTGVCETIPEACT---EQYDPVCGCDDKTYSNACFAHAAGVSVALRGECP 367          

HSP 3 Score: 101.293 bits (251), Expect = 1.530e-19
Identity = 91/245 (37.14%), Postives = 112/245 (45.71%), Query Frame = 0

Query:  295 GVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGG-KGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETECPLVSVQ 537
            G C + P  C     T  +  VCGCDG TY  EC A  AG+S+  RG C     P PA  CGG  GL  E     G +C Y    +C   D  G C   P  CT+E +PVCGC+G TY NAC AA AG S+ S GEC                   A   GG C       C  G++C +A           G C   PE+C       + VCGCDG+++  AC A  AGV++ +  EC    VQ
Sbjct:   83 GTCERIPKVC-----TEEYNPVCGCDGKTYGNECAANSAGVSVASRGEC----EPPPARDCGGLAGLACEP----GEFCDYAPDAMCGAADQTGVCAPIPEGCTKEYNPVCGCDGNTYGNACMAAAAGVSVASPGEC-------------------APAPGGVCGTRGAAACEPGFFCDFAPEAACGATDLPGACAPIPEACT---FEYNPVCGCDGNTYGNACAANAAGVSVASRGECAPSEVQ 292          

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|521002857|gb|AGP35860.1| (hypothetical protein SCE1572_15940 [Sorangium cellulosum So0157-2])

HSP 1 Score: 142.124 bits (357), Expect = 1.287e-33
Identity = 130/389 (33.42%), Postives = 160/389 (41.13%), Query Frame = 0

Query:   43 CPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECP 430
            C  G FC   L    ++ G  +  G  C+  P +C +  +PVCGCDG  Y NEC AN  GVSV S+G C                                 DCGG   + C P   C Y     CG A+ +G C P  +  C  +Y PVCGCDG TY NAC+A       V  PG C  A  G   +  T G    E                      P   C               E  C + +    G CA  P  C     T  +  VCGCDG TY   C A  AG+S+  RG C     P+    CGG  L    C+  G +C Y    +C   D  G C + P  CT++ DPVCGC+ KTY NAC A  AG S+  +GECP
Sbjct:   80 CKKGYFCDYEL---EAMCGAFDMTG-TCERIPKVCTEEYNPVCGCDGKTYGNECAANSAGVSVASRGECEPP---------------------------PARDCGGLAGLACEPGEFCDYAPDAMCGAADQTGVCAPIPE-GCTKEYNPVCGCDGNTYGNACMAAAAGVS-VASPGECAPAPGG---VCGTRGAAACE----------------------PGFFCD-----------FAPEAACGATD--LPGACAPIPEAC-----TFEYNPVCGCDGNTYGNACAANAAGVSVASRGEC----APSEVQHCGG--LAGLACDP-GEFCNYSLEAICGAADQTGVCETIPEACTEQYDPVCGCDDKTYSNACFAHAAGVSVALRGECP 385          

HSP 2 Score: 116.316 bits (290), Expect = 1.266e-24
Identity = 129/384 (33.59%), Postives = 164/384 (42.71%), Query Frame = 0

Query:  152 IKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYC-HVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYA-AGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETECP 532
            + C   + C YE    CG  + +G CE    + C  +Y PVCGCDG TY N C A    GV V   G CE                    PA D             GGL       A   C    +C +  +  C + +    GVCA  P  C     T  +  VCGCDG TY   C A  AG+S+   G C     PAP   CG +G  A    + G++C +     C   D  G C   P  CT E +PVCGC+G TY NAC A  AG S+ S+GEC    +      GGL                AGL C  G +C Y+   +CG+ A   G+C   PE+C         VCGCD  +++ AC A  AGV++    ECP
Sbjct:   78 LSCKKGYFCDYELEAMCGAFDMTGTCERIPKV-CTEEYNPVCGCDGKTYGNECAAN-SAGVSVASRGECE------------------PPPARDC------------GGL-------AGLACEPGEFCDYAPDAMCGAAD--QTGVCAPIPEGC-----TKEYNPVCGCDGNTYGNACMAAAAGVSVASPGEC----APAPGGVCGTRGAAA---CEPGFFCDFAPEAACGATDLPGACAPIPEACTFEYNPVCGCDGNTYGNACAANAAGVSVASRGECAPSEVQH---CGGL----------------AGLACDPGEFCNYSLEAICGA-ADQTGVCETIPEACT---EQYDPVCGCDDKTYSNACFAHAAGVSVALRGECP 385          

HSP 3 Score: 100.908 bits (250), Expect = 2.042e-19
Identity = 91/245 (37.14%), Postives = 112/245 (45.71%), Query Frame = 0

Query:  295 GVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGG-KGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETECPLVSVQ 537
            G C + P  C     T  +  VCGCDG TY  EC A  AG+S+  RG C     P PA  CGG  GL  E     G +C Y    +C   D  G C   P  CT+E +PVCGC+G TY NAC AA AG S+ S GEC                   A   GG C       C  G++C +A           G C   PE+C       + VCGCDG+++  AC A  AGV++ +  EC    VQ
Sbjct:  101 GTCERIPKVC-----TEEYNPVCGCDGKTYGNECAANSAGVSVASRGEC----EPPPARDCGGLAGLACEP----GEFCDYAPDAMCGAADQTGVCAPIPEGCTKEYNPVCGCDGNTYGNACMAAAAGVSVASPGEC-------------------APAPGGVCGTRGAAACEPGFFCDFAPEAACGATDLPGACAPIPEACT---FEYNPVCGCDGNTYGNACAANAAGVSVASRGECAPSEVQ 310          

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|224005785|ref|XP_002291853.1| (probable serine protease inhibitor [Thalassiosira pseudonana CCMP1335] >gi|220972372|gb|EED90704.1| probable serine protease inhibitor [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 136.346 bits (342), Expect = 5.451e-31
Identity = 133/429 (31.00%), Postives = 175/429 (40.79%), Query Frame = 0

Query:    4 FVQIFCGKMRTLSLIATAAGLVGVAYGAEQTCGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSK--NPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCG-GKGLYAETCNKEGYYCLYQRGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGEC 429
            F+Q+       L L  T A     ++ A + C T    +C    +C    GEC   +   +   G C+  P MC    +PVCGCDG  YSNEC+A    VSV S G C                   D D T  +         G +A  C  +  C    G  C    G+G C    D MC  +Y PVCGCD  TY+N+C AQ   G+ V   G C+       ++   +GGQ                                   CG   YC +  G C+ +    + EG C + P  C     T  +  VCGCDG TY  EC A  A +S+   G C   G      +C   KG  A +C K   +C    G C      G CT+ P MCT E DPVCGC+  TY N+C+A   G ++  +GEC
Sbjct:    3 FIQLLTTTPLILVLSPTNAS---ASFLAGKYC-TYTGGQCGRDKYCQLPTGECNKRIA-TDMMEGNCQQPPAMCTADYNPVCGCDGKTYSNECQAFAASVSVASTGKC----------------GNNDRDVTCTLE-------KGGSANSCKSNEFCAGTDG-RCFDG-GNGRCTTMPD-MCTLEYDPVCGCDNLTYANSCEAQSM-GMNVAYRGECD-------NVCTYTGGQ-----------------------------------CGRDKYCQLPTGECNKRIATDMMEGNCQQPPAMC-----TADYNPVCGCDGKTYSNECQAFAASVSVASTGKC---GNNDRDVTCTLEKGGSANSC-KSNEFCAGTDGRC-FDGGNGRCTTMPDMCTLEYDPVCGCDNWTYANSCEAQSMGVNVDYRGEC 347          

HSP 2 Score: 108.997 bits (271), Expect = 1.061e-21
Identity = 127/419 (30.31%), Postives = 166/419 (39.62%), Query Frame = 0

Query:  153 KCAPDHVCIYEAGNECGKAEGS----GHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDC-GLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGVCSLHDSL----GTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETEC-PLVSVQVTSPAMHRYRGRVGGVMVMMTVFG 561
            +C  D  C    G EC K   +    G+C+     MC  DY PVCGCDG TYSN C A +   V V   G C              G  D      D+    E+ G          + C +++ C G  G C   +GG         G C   P  C     T  +  VCGCD  TY   C A+  GM++ YRG C    T       GG+      C ++ Y C    G C+   +     G C   PAMCT + +PVCGC+GKTY N C+A  A  S+ S G+C       G     +T  L    +   CK +         +C    G C       G C   P+ C  +      VCGCD  ++A +C+A   GV +    EC  LV V V      R R    GV     + G
Sbjct:   37 QCGRDKYCQLPTG-ECNKRIATDMMEGNCQQPP-AMCTADYNPVCGCDGKTYSNECQA-FAASVSVASTGKC--------------GNND-----RDVTCTLEKGGS--------ANSCKSNEFCAGTDGRCF--DGG--------NGRCTTMPDMC-----TLEYDPVCGCDNLTYANSCEAQSMGMNVAYRGECDNVCTYT-----GGQ------CGRDKY-CQLPTGECNKRIATDMMEGNCQQPPAMCTADYNPVCGCDGKTYSNECQAFAASVSVASTGKC-------GNNDRDVTCTLEKGGSANSCKSNE--------FCAGTDGRCFD--GGNGRCTTMPDMCTLE---YDPVCGCDNWTYANSCEAQSMGVNVDYRGECEKLVGVCVPD---DRPRPVRNGVRFRFNMNG 375          

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|501192284|ref|WP_012235302.1| (hypothetical protein [Sorangium cellulosum] >gi|161161524|emb|CAN92829.1| hypothetical protein predicted by Glimmer/Critica [Sorangium cellulosum So ce56])

HSP 1 Score: 134.42 bits (337), Expect = 5.604e-31
Identity = 139/394 (35.28%), Postives = 171/394 (43.40%), Query Frame = 0

Query:   39 LAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNC-PKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDA-SEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGEC 429
            L   C  G FC   LG     + G   + G C+  P++C + +  VCGCDG  YSNEC AN  G SV S G C P  G +                            CGG   + C     C +    +CG  + +G C PT    C  +Y PVCGCDG TY NAC+A    GV V   G C    SEG A    T GG   E+   +  +   EV                         C   +G          GVC   P  C     T  +  VCGCDG TY   C A  AG+S+  +G C     PAP   CGG   +A  C K G +C Y    +C   D  GTC + P  CT++ DPVCGC+G+TY NAC AA AG S+ SKGEC
Sbjct:   59 LGLSCKKGLFCDYELG----AMCGAADQTGTCQPIPEVCTKELREVCGCDGNTYSNECMANAAGTSVSSIGQCAPPPGDV----------------------------CGGLLGLSCKEGEFCDFAPDAQCGAGDMTGTCLPTPG-GCTKEYAPVCGCDGNTYGNACMANAA-GVSVASKGECAPPPSEGKA--CGTRGGWTCEK--GEFCNFPPEV------------------------ECGAWDG---------PGVCTPIPEAC-----TFEYNPVCGCDGQTYGNACAAASAGVSVASKGEC----APAPGKICGGIAGFA--CEK-GEFCNYSIEAMCGAADQTGTCETIPEACTEQYDPVCGCDGQTYGNACAAASAGVSVASKGEC 369          

HSP 2 Score: 120.553 bits (301), Expect = 3.264e-26
Identity = 128/381 (33.60%), Postives = 166/381 (43.57%), Query Frame = 0

Query:  152 IKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPADSCGGKGLYAETCNKEGYYCLYQRGV-CSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVALKAETEC 531
            + C     C YE G  CG A+ +G C+P  ++ C  +   VCGCDG TYSN C+A    G  V   G C           D  GG         L+  + + GE  +    P  +C A     + G C    GGC                       T  +  VCGCDG TY   C A  AG+S+  +G C     P+   +CG +G +  TC K G +C +   V C   D  G CT  P  CT E +PVCGC+G+TY NAC AA AG S+ SKGEC                   A   G  C   AG  C +G +C Y+       A   G C   PE+C         VCGCDG ++  AC AA AGV++ ++ EC
Sbjct:   61 LSCKKGLFCDYELGAMCGAADQTGTCQPIPEV-CTKELREVCGCDGNTYSNECMANAA-GTSVSSIGQCAPPP------GDVCGG---------LLGLSCKEGEFCD--FAPDAQCGAGD---MTGTCLPTPGGC-----------------------TKEYAPVCGCDGNTYGNACMANAAGVSVASKGECAP--PPSEGKACGTRGGW--TCEK-GEFCNFPPEVECGAWDGPGVCTPIPEACTFEYNPVCGCDGQTYGNACAAASAGVSVASKGEC-------------------APAPGKICGGIAGFACEKGEFCNYSIEAMCGAADQTGTCETIPEACT---EQYDPVCGCDGQTYGNACAAASAGVSVASKGEC 369          

BLAST of EWM25526.1 vs. NCBI_GenBank
Match: gi|921301958|ref|WP_053237166.1| (hypothetical protein [Sandaracinus amylolyticus])

HSP 1 Score: 120.168 bits (300), Expect = 9.199e-26
Identity = 102/317 (32.18%), Postives = 131/317 (41.32%), Query Frame = 0

Query:   28 AYGAEQTCGTRLAPECPDGSFCSTGLGECTSLLGGLNTKGGMCKLKPDMCAQFVDPVCGCDGVDYSNECEANMKGVSVQSKGNCPKSGGLNNLRPGIGGNAEGDHDSTAAIYGTSTTDCGGPTAIKCAPDHVCIYEAGNECGKAEGSGHCEPTKDIMCMTDYTPVCGCDGTTYSNACVAQYQNGVPVRGPGTCEDASEGTADIADTSGGQDVEEPANDLIDGAEEVGEEREGGLFPTHKCTADKDCGLHGYCHVQEGGCSSKNPITEGVC-AKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGAC 343
            A G++Q CG+R  PEC +G FC+   G     + G + +GG+C  +PD C +   PVCGCDG  Y N C A   GVSV   G CP  GG            EG+              CG      C     C +     CG+A+ +G C    D  C+  Y PVCGCDG TY NAC A   NG+ V   G CE   +G     +T GG                                A  +C     C     GC+  +    GVC   +P  C     T  +  VCGCDG TY  +C  R A + + + GAC
Sbjct:  171 APGSQQACGSRGLPECGEGFFCAFEAGS----MCGADDRGGVCARRPDACIEIYQPVCGCDGQTYGNACSAASAGVSVAHDGECPAPGG------------EGEP-------------CGARLGDTCDEGFYCAFAPEAICGRADATGTCARRPD-ACIQVYDPVCGCDGRTYGNACTAA-SNGMSVDHAGECERVGQGE---GETCGG-------------------------------IAALECAAGLACDYSASGCNIAD--VAGVCVVDEPRAC-----TREYRPVCGCDGNTYSNDCVRRAAYVGLAHTGAC 415          

HSP 2 Score: 118.242 bits (295), Expect = 4.132e-25
Identity = 88/236 (37.29%), Postives = 119/236 (50.42%), Query Frame = 0

Query:  295 GVCAKKPLRCESPPFTTAFTAVCGCDGTTYPTECHARQAGMSILYRGACFRQGTPAPA---DSCGGKGLYAETCNKEGYYCLYQ-RGVCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVV-QPESCVPDPSSAHSVCGCDGHSWATACDAAMAGVAL 525
            GVCA++P  C        +  VCGCDG TY   C A  AG+S+ + G C     PAP    + CG +    +TC+ EG+YC +    +C   D+ GTC  +P  C Q  DPVCGC+G+TY NAC AA  G S+   GEC  V   +G   GG+                A L C+ G  C Y+A  C + A   G+CVV +P +C  +      VCGCDG++++  C    A V L
Sbjct:  207 GVCARRPDAC-----IEIYQPVCGCDGQTYGNACSAASAGVSVAHDGEC-----PAPGGEGEPCGAR--LGDTCD-EGFYCAFAPEAICGRADATGTCARRPDACIQVYDPVCGCDGRTYGNACTAASNGMSVDHAGECERVGQGEGETCGGI----------------AALECAAGLACDYSASGC-NIADVAGVCVVDEPRACTRE---YRPVCGCDGNTYSNDCVRRAAYVGL 409          

HSP 3 Score: 103.99 bits (258), Expect = 2.676e-20
Identity = 77/220 (35.00%), Postives = 102/220 (46.36%), Query Frame = 0

Query:  322 TTYPTECHARQAGMSILYRGACFRQ----GTPAPADSCGGKGLYAETCNKEGYYCLYQRG-VCSLHDSLGTCTSKPAMCTQEIDPVCGCNGKTYDNACKAAMAGSSIKSKGECPFVTIDDGWGGGGLTIGLLANVTGGPCKPSAGLTCSEGYYCTYAAGVCGSEATAEGLCVVQPESCVP--DPSSAHSVCGCDGHSWATACDAAMAGVALKAETECPLV 534
            T+Y      R   MS     +C         P    +CG +GL    C  EG++C ++ G +C   D  G C  +P  C +   PVCGC+G+TY NAC AA AG S+   GECP          GG          G PC    G TC EG+YC +A       A A G C  +P++C+   DP     VCGCDG ++  AC AA  G+++    EC  V
Sbjct:  141 TSYSYAVRNRPEAMSFHLTVSCNGADGECAAPGSQQACGSRGL--PECG-EGFFCAFEAGSMCGADDRGGVCARRPDACIEIYQPVCGCDGQTYGNACSAASAGVSVAHDGECP--------APGG---------EGEPCGARLGDTCDEGFYCAFAPEAICGRADATGTCARRPDACIQVYDP-----VCGCDGRTYGNACTAASNGMSVDHAGECERV 335