EWM25616.1, cds5055 (CDS) Nannochloropsis gaditana

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|585107440|gb|EWM25616.1| (major facilitator superfamily protein [Nannochloropsis gaditana])

HSP 1 Score: 1281.16 bits (3314), Expect = 0.000e+0
Identity = 635/635 (100.00%), Postives = 635/635 (100.00%), Query Frame = 0

Query:    1 MNKNDTSTHFSEAYYAVPSSSPHSEDVGNVEPRASPTEQNRSIVPSANKALVDITLSPPRGEDEKSTVVVRSENEIVPTDIKATSSSSSLSTSGTPPCHVLGRRSSRHSGYTLALLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREEQVLIEMVECPIAVEEETVAAYREAARMEEEEEEEEEGEQRGKEGETGAVAARPSHPRSFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVGAVVGGCTPMLASALSASPIFLAPILPLSAVAAAVAVVLGRYEWGEYVKRKAEGGKGGREEFLALRVEEGKEGKGKMAKAKVKARGEEPADTS 635
            MNKNDTSTHFSEAYYAVPSSSPHSEDVGNVEPRASPTEQNRSIVPSANKALVDITLSPPRGEDEKSTVVVRSENEIVPTDIKATSSSSSLSTSGTPPCHVLGRRSSRHSGYTLALLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREEQVLIEMVECPIAVEEETVAAYREAARMEEEEEEEEEGEQRGKEGETGAVAARPSHPRSFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVGAVVGGCTPMLASALSASPIFLAPILPLSAVAAAVAVVLGRYEWGEYVKRKAEGGKGGREEFLALRVEEGKEGKGKMAKAKVKARGEEPADTS
Sbjct:    1 MNKNDTSTHFSEAYYAVPSSSPHSEDVGNVEPRASPTEQNRSIVPSANKALVDITLSPPRGEDEKSTVVVRSENEIVPTDIKATSSSSSLSTSGTPPCHVLGRRSSRHSGYTLALLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREEQVLIEMVECPIAVEEETVAAYREAARMEEEEEEEEEGEQRGKEGETGAVAARPSHPRSFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVGAVVGGCTPMLASALSASPIFLAPILPLSAVAAAVAVVLGRYEWGEYVKRKAEGGKGGREEFLALRVEEGKEGKGKMAKAKVKARGEEPADTS 635          

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|553186800|ref|XP_005854492.1| (major facilitator superfamily mfs 1 [Nannochloropsis gaditana CCMP526] >gi|422294564|gb|EKU21864.1| major facilitator superfamily mfs 1 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1266.91 bits (3277), Expect = 0.000e+0
Identity = 629/635 (99.06%), Postives = 632/635 (99.53%), Query Frame = 0

Query:    1 MNKNDTSTHFSEAYYAVPSSSPHSEDVGNVEPRASPTEQNRSIVPSANKALVDITLSPPRGEDEKSTVVVRSENEIVPTDIKATSSSSSLSTSGTPPCHVLGRRSSRHSGYTLALLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREEQVLIEMVECPIAVEEETVAAYREAARMEEEEEEEEEGEQRGKEGETGAVAARPSHPRSFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVGAVVGGCTPMLASALSASPIFLAPILPLSAVAAAVAVVLGRYEWGEYVKRKAEGGKGGREEFLALRVEEGKEGKGKMAKAKVKARGEEPADTS 635
            MNKNDTSTHFSEAYYAVPSSSPHSEDVGNVEPRASPTEQNRSIVPSANKALVDITLSPPRGEDEKSTVVVRSENEIVPTDIKATSSSSSLSTSGTPPCHVLGRRSSRHSGYTLALLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREE+VLIEMVECPIAVEEETVAAYREAARM EEEEEEEEGEQRGKEGE GAVAARPSHPRSFCGI+REAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVGAVVGGCTPMLASALSASPIFLAPILPLSAVAAAVAVVLGRYEWGEYVKRK EGGKGGREEFLALRVEEGKEGKGKMAKA+VKARGEEPADTS
Sbjct:    1 MNKNDTSTHFSEAYYAVPSSSPHSEDVGNVEPRASPTEQNRSIVPSANKALVDITLSPPRGEDEKSTVVVRSENEIVPTDIKATSSSSSLSTSGTPPCHVLGRRSSRHSGYTLALLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREERVLIEMVECPIAVEEETVAAYREAARM-EEEEEEEEGEQRGKEGEAGAVAARPSHPRSFCGIMREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVGAVVGGCTPMLASALSASPIFLAPILPLSAVAAAVAVVLGRYEWGEYVKRKTEGGKGGREEFLALRVEEGKEGKGKMAKAEVKARGEEPADTS 634          

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|553186812|ref|XP_005854495.1| (major facilitator family transporter [Nannochloropsis gaditana CCMP526] >gi|422294567|gb|EKU21867.1| major facilitator family transporter [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 341.273 bits (874), Expect = 3.275e-104
Identity = 164/226 (72.57%), Postives = 195/226 (86.28%), Query Frame = 0

Query:  104 RSSRHSGYTLALLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGE--AGRRPTREEQ 327
            +  R + YT+A LAAIMGNVLEFYDFS+YA+FAPEIG+LFF  GS T++ ++TFTVFAGGFLMRP+GSVVLGAVGDKYGTAAALKWSI LMT+PT+VTGLLPTY K+GLAAPLLLTLCRL+QGLSVGGELIGALLHTADHSP   RG +CG+V VFGS+GNL+G++VSV MR AL+++ELLSWGWRIPFL GSVLG VG+ LRT+T  L+   E  A +R  +E++
Sbjct:   51 KIKRPNHYTMATLAAIMGNVLEFYDFSIYAYFAPEIGKLFFPKGSPTIQVVKTFTVFAGGFLMRPLGSVVLGAVGDKYGTAAALKWSIALMTIPTVVTGLLPTYHKVGLAAPLLLTLCRLVQGLSVGGELIGALLHTADHSPPGRRGLYCGIVGVFGSVGNLIGAVVSVSMRKALTERELLSWGWRIPFLTGSVLGFVGVLLRTQTDGLRSKEEHKAKKRVKKEQK 276          

HSP 2 Score: 196.823 bits (499), Expect = 1.798e-50
Identity = 98/187 (52.41%), Postives = 126/187 (67.38%), Query Frame = 0

Query:  384 SFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVGAVVGGCTPMLASALSASPI-FLAPILP 569
            SF  ILR+AA  DK RF+ VV+LA L +S FY +FVW+GTY+A LA  P+ +  AFSV  V Q + I   PFWG MGD  GG   R++W I G+V    + P  F LL  G  S +F +A + GL MG ++ GNY+AWV E+L +SP+RVL+TSVAYN+GA+ GGC PML SALSA     +AP+LP
Sbjct:  474 SFLQILRDAAGTDKARFLHVVLLATLQSSQFYLHFVWVGTYLAVLARKPITSVAAFSVITVMQVLLILTGPFWGLMGDKYGGAKGRVKWLIAGIVCDIIMVPTAFSLLERGGESWAFAYAVMVGLTMGLSLGGNYHAWVTETLQDSPARVLSTSVAYNLGAMTGGCAPMLYSALSAYRFPQIAPVLP 660          

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|585107444|gb|EWM25620.1| (major facilitator family transporter [Nannochloropsis gaditana])

HSP 1 Score: 340.887 bits (873), Expect = 4.500e-104
Identity = 164/226 (72.57%), Postives = 195/226 (86.28%), Query Frame = 0

Query:  104 RSSRHSGYTLALLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGE--AGRRPTREEQ 327
            +  R + YT+A LAAIMGNVLEFYDFS+YA+FAPEIG+LFF  GS T++ ++TFTVFAGGFLMRP+GSVVLGAVGDKYGTAAALKWSI LMT+PT+VTGLLPTY K+GLAAPLLLTLCRL+QGLSVGGELIGALLHTADHSP   RG +CG+V VFGS+GNL+G++VSV MR AL+++ELLSWGWRIPFL GSVLG VG+ LRT+T  L+   E  A +R  +E++
Sbjct:   51 KIKRPNHYTMATLAAIMGNVLEFYDFSIYAYFAPEIGKLFFPRGSPTIQVVKTFTVFAGGFLMRPLGSVVLGAVGDKYGTAAALKWSIALMTIPTVVTGLLPTYHKVGLAAPLLLTLCRLVQGLSVGGELIGALLHTADHSPPGRRGLYCGIVGVFGSVGNLIGAVVSVSMRKALTERELLSWGWRIPFLTGSVLGFVGVLLRTQTDGLRSKEEHKAKKRVKKEQK 276          

HSP 2 Score: 197.208 bits (500), Expect = 1.122e-50
Identity = 106/233 (45.49%), Postives = 142/233 (60.94%), Query Frame = 0

Query:  384 SFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVGAVVGGCTPMLASALSASPI-FLAPILPLSAVAAAVAVVL--------GRYEWGEYVKRKAEG---GKGGREEF 604
            SF  ILR+AA  DK RF+ VV+LA L +S FY +FVW+GTY+A LA  P+ +  AFSV  V Q + I   PFWG MGD  GG   R++W I G+V    + P  F LL  G  S +F +A + GL MG ++ GNY+AWV E+L +SP+RVL+TSVAYN+GA+ GGC PML SALSA     +AP+LP       V  +         G +   ++ +++ +G   G GG EE 
Sbjct:  474 SFLQILRDAAGTDKARFLHVVLLATLQSSQFYLHFVWVGTYLAVLARKPITSVAAFSVITVMQVLLILTGPFWGLMGDKYGGAKGRVKWLIAGIVCDIIMVPTAFSLLERGGESWAFAYAVMVGLTMGLSLGGNYHAWVTETLQDSPARVLSTSVAYNLGAMTGGCAPMLYSALSAYRFPQIAPVLPPIVYGGLVLAIFIYSRASPRGIFHVSKWEEKREQGDLDGMGGGEEL 706          

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|953246016|emb|CUS39067.1| (General substrate transporter [Candidatus Nitrospira nitrificans])

HSP 1 Score: 198.364 bits (503), Expect = 3.769e-53
Identity = 151/445 (33.93%), Postives = 214/445 (48.09%), Query Frame = 0

Query:  115 LLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREEQVLIEMVECPIAVEEETVAAYREAARMEEEEEEEEEGEQRGKEGETGAVAARPSHPRSFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPC-LFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNV-GAVVGGCTPMLASAL 557
            +LA  +GNVLE+YDF+++ +FAP + +LFF +   ++  I TF VFA GFL RP+G+++ G  GD  G  AAL WSI LM  PT + GLLPTY++IGLAAPL LT+ R LQGLSVGGE  G++    +H+    RG+          +G L+GS V  L    LS + L  WGWRIPF+AGSV+ LVG +LR                                             R   E    E  +Q      TGAV++ P         +RE     +   ++V+ L  L+   FY  FV+L TY+ ++   P+G     +  C+A    +   P  G + D  G      RW + G      L    LF  L  G  +L  +   L  L +     G ++A V  +L    SR    S++YN+  A+ GG  P++A+ L
Sbjct:   30 VLAGAVGNVLEWYDFALFGYFAPVLSRLFFPASDPSLSLIATFGVFAVGFLARPLGALLFGYWGDTKGRRAALAWSIILMAFPTCLVGLLPTYAQIGLAAPLALTVLRFLQGLSVGGEFTGSVTFLVEHAGPTERGYVGSWAGFSAQIGALLGSGVGTLATANLSSEALQQWGWRIPFVAGSVIALVGWYLR---------------------------------------------RRIPESPAFERLQQ------TGAVSSSP---------VRELLTSHRAPLLQVIGLVLLHGVGFYIFFVFLPTYLTKVTDLPMGTTLLINTGCMALLAILI--PLMGKLSDRVGH-----RWVLAGGAAGLALGTVPLFSWLSGGHLALIVMAQVLITLFVS-AYMGPFFA-VVATLFPVASRYTGLSISYNIAAALFGGTAPLMATVL 405          

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|517529180|ref|WP_018699388.1| (MFS transporter [Amorphus coralli])

HSP 1 Score: 190.66 bits (483), Expect = 1.486e-50
Identity = 151/448 (33.71%), Postives = 207/448 (46.21%), Query Frame = 0

Query:  116 LAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREEQVLIEMVECPIAVEEETVAAYREAARMEEEEEEEEEGEQRGKEGETGAVAARPSHPRSFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTV-----SGNYYAWVCESLNESPSRVLTTSVAYNVG-AVVGGCTPMLASAL 557
            +A  +GN LE+YDF+VY FFAP I   FF      V  I TF VFA GFLMRP G +V+G V DK G  AAL  S+ +M +PT +   LP Y  IGLAAPLLLT  RL+QGLSVGGE  G++ +  ++ P + RG       V  ++G L+G+ +  ++   +  + L SWGWRIPFL G +LGL G++ R+                                               E+ E E       +E E   V A   H R                 +RV+ +  LN   FY  FV+L TY+     + +   +A  +     AV     PF+ A+ D    + +R       + ++ F  P LFL L SG      L+  L G L GF V     +G     + E       RV   SVA+N+  A+VGG TPM+A+ L
Sbjct:   10 VAGAIGNTLEWYDFAVYGFFAPIIATQFFPKTDPKVALIATFGVFAAGFLMRPFGGLVIGHVADKLGRRAALTLSVAMMAIPTTLVAFLPIYEMIGLAAPLLLTFLRLVQGLSVGGEYTGSVTYLVENGPQDQRGISGSFGLVGSNLGILLGAAMGAIVTALVPDEALASWGWRIPFLCGVLLGLAGVYFRSHV---------------------------------------------EDIEVEFNDPHEPQEPEFPIVEAVRDHWRG---------------MLRVMGINVLNGIGFYIAFVYLTTYIVTY--DGVNENRALDINTGVIAVLCLVIPFFAALSD---RIGRRPVMMTGALGVAVFAYP-LFLALGSGT-----LWGILLGQL-GFAVLMACFTGPLPTTMTEQFPRH-VRVTAYSVAFNIPMAIVGGTTPMVATWL 384          

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|1111737698|gb|OJJ24145.1| (MFS transporter [Roseofilum reptotaenium AO1-A])

HSP 1 Score: 187.963 bits (476), Expect = 1.101e-49
Identity = 90/194 (46.39%), Postives = 132/194 (68.04%), Query Frame = 0

Query:  115 LLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTK 308
            +LA ++GNV+E+YDF++Y + AP I  LFF + +  V  +QT+ VFA GF+MRP+G+ + G +GD+      L  S+ LM +PT + GLLPTY++IG+AAP+ L L RL+QGLSVGGE  G++ + A+ +P   RGF    V V  + G L+G  +  L+ H LS   LL+WGWR+PFL G +LGL G+++R+ 
Sbjct:    5 ILAGVVGNVIEWYDFALYGYLAPIISLLFFPNDNQIVSLLQTYGVFAAGFVMRPLGAGIFGYIGDRVSRRTELFISVILMAIPTFILGLLPTYNQIGIAAPIFLILLRLVQGLSVGGEFTGSVTYVAETAPQTRRGFTTSFVNVGSTTGLLLGLGIVALITHLLSDSNLLTWGWRLPFLFGGILGLTGLYIRSN 198          

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|692232082|ref|WP_032112454.1| (MFS transporter [Candidatus Paracaedibacter symbiosus])

HSP 1 Score: 186.808 bits (473), Expect = 3.602e-49
Identity = 82/188 (43.62%), Postives = 132/188 (70.21%), Query Frame = 0

Query:  121 GNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTK 308
            GN+LE+Y+F+++ F  P I QLFF S + T   + T+ +FA GF+MRP+G+++ G +GD YG   +L  S+ +MT+PT   GLLPT+++IG+ AP L+  CRL+QG+S+GGE  G++++  +++P + RGFF     +  SMG ++ +L S+ +  AL KQ++L WGWR+PFL G + G++G+ +R K
Sbjct:   20 GNLLEWYEFAIFGFLTPYISQLFFPSDNPTTSLLLTYGIFATGFVMRPLGAIIAGHIGDLYGRKFSLVLSLLMMTIPTTAIGLLPTFNQIGIYAPFLILCCRLIQGISLGGEFSGSIIYLIENAPPQRRGFFGSWADMGSSMGMILATLTSLALTFALDKQQILDWGWRLPFLLGIIFGILGLVMRRK 207          

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|488723405|ref|WP_002647110.1| (MFS transporter [Gimesia maris] >gi|148844402|gb|EDL58754.1| major facilitator family transporter [Gimesia maris DSM 8797])

HSP 1 Score: 184.111 bits (466), Expect = 5.015e-48
Identity = 144/454 (31.72%), Postives = 216/454 (47.58%), Query Frame = 0

Query:  115 LLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREEQVLIEMVECPIAVEEETVAAYREAARMEEEEEEEEEGEQRGKEGETGAVAARPSHPRSFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIFGNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFL-------LLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVG-AVVGGCTPMLASALSAS 560
            L A  +GN+LE+YDF+VY FFAP IG+LFF S    V  I  F  FA GFLMRPVG+V+ G +GD+ G   AL  S+ +M +PT++ G+LPT+++IG+ A +++ L R++QG+SVGGE   + +   +H+PA  R FF     +  + G L+GS V  L+    + ++L+ WGWRIPFLAG ++  VG F+R       HG                + + PIA               EE  E+E               A  P         L++A A  K   ++   L  +N   FY  F++L T++ E        A+A  +  ++ A      PF   + D   G    L +   GV L  +  P L+L       ++ +G+   + L A   G +                L +   RV   SV+YN+  A+ GG  PM+A+ L  S
Sbjct:   21 LAAGFIGNILEWYDFAVYGFFAPTIGKLFFPSEDPRVSLIAAFGAFAAGFLMRPVGAVLFGHIGDRIGRKKALTLSVLMMAIPTMLVGILPTHAQIGMYAAIMMVLLRMIQGISVGGEYTSSFVFLVEHAPAGRRAFFGSWSMIGATCGILLGSAVGALINTFTTNEQLMDWGWRIPFLAGVLVAFVGYFIR-------HG----------------IPDQPIA---------------EELSEQE---------------AYSP---------LKQAWASYKTELLQSAGLNMMNAVTFYTVFIYLSTWLVEEVGET--RAEALDINTISMAALTVMVPFAAMLAD-KYGRKPLLLFGAAGVTLFSY--PLLWLMHHQHYQMILAGQIGFAVLVACFAGAIPA----------TITELFKRGVRVSAASVSYNIPFAIFGGTAPMVAAWLVHS 397          

BLAST of EWM25616.1 vs. NCBI_GenBank
Match: gi|664477441|ref|WP_030998000.1| (MFS transporter [Streptomyces sp. NRRL F-5630])

HSP 1 Score: 185.652 bits (470), Expect = 6.500e-48
Identity = 146/453 (32.23%), Postives = 212/453 (46.80%), Query Frame = 0

Query:  114 ALLAAIMGNVLEFYDFSVYAFFAPEIGQLFFSSGSDTVRAIQTFTVFAGGFLMRPVGSVVLGAVGDKYGTAAALKWSIGLMTVPTIVTGLLPTYSKIGLAAPLLLTLCRLLQGLSVGGELIGALLHTADHSPAEHRGFFCGLVAVFGSMGNLVGSLVSVLMRHALSKQELLSWGWRIPFLAGSVLGLVGIFLRTKTGALKHGGEAGRRPTREEQVLIEMVECPIAVEEETVAAYREAARMEE---EEEEEEEGEQRGKEGETGAVAARPSHPRSFCGILREAAAGDKPRFIRVVVLAALNNSLFYFNFVWLGTYMAELAPNPLGAAQAFSVTCVAQAVFIF-----GNPFWGAMGDWGGGLAKRLRWNIVGVVLSCFLPPCLFLLLRSGRASLSFLFAALTGLLMGFTVSGNYYAWVCESLNESPSRVLTTSVAYNVG-AVVGGCTPMLASAL 557
            A+ AA +GN +E++DF VYA+ A  +G++FF S S   + +QTF  FA  FL+RP+G +V G +GD+ G    L  +I +M V T   G+LPTY+ +G AAP+LL +CRL+QG S GGE  GA  + A+++P   RGFF   +     +G  +GS +  ++   L +  L  WGWRIPF     LG++G++LR K                                EET A  RE    EE   E++  EE  Q GK                  G LR+         +  + L  L N   Y    +L TYM+E        AQ   +  +   V        G+  WG    +  G       +I  +VLS    P  FLL+R G   L  + + + GLL+  T +G   A    +L  +  R    S+A+N+  ++ GG TP+ ASAL
Sbjct:   29 AVTAAALGNTIEWFDFGVYAYLAGTLGKVFFPSSSPGAQVVQTFATFAAAFLVRPLGGLVFGPLGDRIGRQKVLAVTIIMMAVSTFAVGVLPTYASVGFAAPILLLVCRLVQGFSTGGEYAGATTYIAEYAPDRRRGFFGSWLDFGTFVGYSLGSGLVTVLTTVLGEDGLTDWGWRIPFFVAGPLGVIGLYLRLKL-------------------------------EETPAFQREEEDREEALAEDDPVEEARQSGK------------------GRLRDIFVHHWEAILICMGLVLLYNVTNYMVTSYLPTYMSETLDESETTAQLLVLGTMVLVVLTITTVGRGSDRWGRRPVFFFG-------SIALIVLSI---PA-FLLIRQGGILLPAIGSGILGLLL-VTFAGT-SASTLPALFPTRIRYGALSIAFNISVSLFGGTTPLFASAL 419