EWM25916.1, cds4762 (CDS) Nannochloropsis gaditana

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|585107785|gb|EWM25916.1| (insulin degrading metalloproteinase [Nannochloropsis gaditana])

HSP 1 Score: 2248.01 bits (5824), Expect = 0.000e+0
Identity = 1088/1088 (100.00%), Postives = 1088/1088 (100.00%), Query Frame = 0

Query:    1 MFKPQNSTGSSFSSSPILTNATGLRRAKSNHSFSTTGCTASGINAIGGSSARGGWYLEDDEEDGFEERRERSNSFGAGGSCSVVRSNSLPFQKTKSNSSLAMMSLTGSLTRIGSNSGSNSSLLRSLHSSSRRWTSLIRSMEHLWDREGGQGMGMETSPQDTDRSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVPPAFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDREPLAVREGDGERTAAVRAGWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHVDSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAAAELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVCLNKEKRRVVSVMMHGKHHRPMRILGGEGTTGKDGPNLRGRVVYLKEEDCLAFRAAHALFPCSPSMFTSKY 1088
            MFKPQNSTGSSFSSSPILTNATGLRRAKSNHSFSTTGCTASGINAIGGSSARGGWYLEDDEEDGFEERRERSNSFGAGGSCSVVRSNSLPFQKTKSNSSLAMMSLTGSLTRIGSNSGSNSSLLRSLHSSSRRWTSLIRSMEHLWDREGGQGMGMETSPQDTDRSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVPPAFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDREPLAVREGDGERTAAVRAGWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHVDSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAAAELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVCLNKEKRRVVSVMMHGKHHRPMRILGGEGTTGKDGPNLRGRVVYLKEEDCLAFRAAHALFPCSPSMFTSKY
Sbjct:    1 MFKPQNSTGSSFSSSPILTNATGLRRAKSNHSFSTTGCTASGINAIGGSSARGGWYLEDDEEDGFEERRERSNSFGAGGSCSVVRSNSLPFQKTKSNSSLAMMSLTGSLTRIGSNSGSNSSLLRSLHSSSRRWTSLIRSMEHLWDREGGQGMGMETSPQDTDRSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVPPAFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDREPLAVREGDGERTAAVRAGWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHVDSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAAAELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVCLNKEKRRVVSVMMHGKHHRPMRILGGEGTTGKDGPNLRGRVVYLKEEDCLAFRAAHALFPCSPSMFTSKY 1088          

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|553177437|ref|XP_005852468.1| (insulysin [Nannochloropsis gaditana CCMP526] >gi|422296065|gb|EKU23364.1| insulysin [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1430.23 bits (3701), Expect = 0.000e+0
Identity = 693/702 (98.72%), Postives = 697/702 (99.29%), Query Frame = 0

Query:  388 EGDGERTAAV-RAGWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHVDSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAAAELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVCLNKEKRRVVSVMMHGKHHRPMRILGGEGTTGKDGPNLRGRVVYLKEEDCLAFRAAHALFPCSPSMFTSKY 1088
            EG+G   + + RAGWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHVDSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAAAELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRL+REKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVCLNKEKRRVVSVMMHGKHHRPMRILGGEGTTGKDGPNLRGRVVYLKEEDCLAFRAAHALFPCSPSMFTSKY
Sbjct:  205 EGEGSIFSVLHRAGWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHVDSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAAAELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLTREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVCLNKEKRRVVSVMMHGKHHRPMRILGGEGTTGKDGPNLRGRVVYLKEEDCLAFRAAHALFPCSPSMFTSKY 906          

HSP 2 Score: 200.29 bits (508), Expect = 3.580e-49
Identity = 96/97 (98.97%), Postives = 96/97 (98.97%), Query Frame = 0

Query:  302 MWQITKAVATPSHPWSKFSTGSLQTLKEDVPPAFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDREPLAVREGDGERTAAVR 398
            MWQITKAVATPSHPWSKFSTGSLQTLKEDVPPAFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDREPLAVREGDG RTAAVR
Sbjct:    1 MWQITKAVATPSHPWSKFSTGSLQTLKEDVPPAFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDREPLAVREGDGGRTAAVR 97          

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|1069816163|ref|XP_018331415.1| (PREDICTED: insulin-degrading enzyme [Agrilus planipennis] >gi|1069816166|ref|XP_018331416.1| PREDICTED: insulin-degrading enzyme [Agrilus planipennis])

HSP 1 Score: 494.582 bits (1272), Expect = 3.419e-154
Identity = 343/1036 (33.11%), Postives = 505/1036 (48.75%), Query Frame = 0

Query:  109 LTRIGSNSGSNSSLLRSLHSSSRRWTSLIRSMEHLWDREG--GQGMGMETSPQDTDRSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVPP---AFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDREPLAVR-----------------------------------------------------EGDGERTAAVRA-GWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHV-DSLHLPRPNPFVAEEFEILAPSYEPPSATPSP-PALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPA---AAELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVCLNK--EKRRVVSVMMHGKHHRPMRILGGEGTTGKDGPNLRGRVVYLKEEDCLAFRAAHALFP 1078
            L R      +N  LL     SS+R  S I++M    +      +   +  SP+D  RSYR L L N+++ +LVSDPTTDKSAAAL+V VG   DP   PGLAHFCEHMLFLGT+KYP EN Y   +++  GSSNA T  D T+Y F  + D     LD FAQFF+ PLFTESAT REL AV++E+ KNL ND  RM Q+ K    P+HP+ KF TG+ +TL  DV P     ++R  LL+F+ + Y +  MALA++ ++SLD LE  V + F  I++++  A R                                                     EG G   +A++  GW++ + AG          F +TV LTEEGL H  D+  L+FQY++++    P E +   +E + I+A  FRF+ K S  +Y + L   LQ +    VL   +         ++  L   T +K  + +  K      F +   D               EPWY   Y+LE  P     +W  A   +   LP  N F+   F I     EP     +  PA+V       VW K D  +  P+A+++ +F +PL   DP       + V+  +  L E  Y   +AGL W +S+   GL L   G+S K   LL K++E + +  I    F + +E  I   +N A  +P +H   +L++LLTE  W  +E L   + L  E L  F  +++++  +   + GN+S++  L L ++VE  +  +    PL P     +R + LD G  +     V N+    S +  YFQ GL        L L AQI++ PCF  LRT+EQLGYIV SGV+     + V GL   V S  H PE +  R+E FL    + ++  +S  E ERHKEAL    LE PK++  ++ + W+EI +    + R  +    +R+  +DD+I  +  + L+K  ++R+ +SV      H      GG GT+       +G V  +  ED   F+++H +FP
Sbjct:   10 LKRFTCIKSTNQLLL-----SSKRSISRIKNMTGQTEARAPLKKFQNIIKSPEDK-RSYRGLELSNHMKVLLVSDPTTDKSAAALNVNVGFLSDPDYLPGLAHFCEHMLFLGTEKYPNENEYIKFLSEHSGSSNASTYTDHTVYYFDVAPDKLTEALDRFAQFFIAPLFTESATERELIAVNSEHEKNLPNDMWRMSQLEKHTCNPNHPYHKFGTGNKETL--DVIPKKKGINVRSELLKFHEQWYSSNIMALAVLGKESLDDLEAIVLELFSNIQNKDIEAPRWSDHPFSDEQLRTCTYMVPIKDIRNLSINFPTEDLNEYYKSSPEGYISHLIGHEGPGSLLSALKTKGWSNSLVAGSRPGGRGLGFFGITVDLTEEGLQHTNDIIKLVFQYINMLKDVGPLEWI--QEEQKDIAAMIFRFKDKESPRTYISGLVHHLQDYPIEDVLCIHYHLTEWRPDIINNILNSFTPDKVRIGVIAKK-----FQKEATD--------------AEPWYGTQYKLEKIPQEVLNDWTNAGTSNEFRLPDKNDFIPTNFNI-----EPLDKDITEHPAIVQDTALTRVWFKQDDKFLLPKANLLFDFVSPLAYLDPMNCNMTHMLVQLFKDALNEYAYAAEIAGLKWELSNTKYGLILEVGGYSDKQHILLRKIMEKLTSFKIDPKRFEIFKENYIRQLKNFAAEQPYQHAVYYLAVLLTEHSWTKEELLASTDQLTIERLEAFIPQMLSKLHIECLLHGNLSKKRGLELVDIVETSLTSSFCMSPLLPRQLLLNRELKLDDGCSY--LYEVQNNVHKLSCIELYFQCGLQNTENNVLLELFAQIIQVPCFNVLRTQEQLGYIVFSGVRR---SNGVQGLRVIVQSDRH-PEYLDTRIELFLESMLKHVME-MSNEEFERHKEALALQRLEKPKRLSVQSQIFWQEITSQQYHFDRANVEVAHLRKLTKDDIINYYQYL-LDKAAKQRKKISV------HVVSMADGGAGTSNSPKKEEKGSVEVI--EDVTVFKSSHGMFP 995          

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|1130260516|ref|XP_019769449.1| (PREDICTED: insulin-degrading enzyme [Dendroctonus ponderosae])

HSP 1 Score: 488.419 bits (1256), Expect = 7.932e-152
Identity = 314/986 (31.85%), Postives = 483/986 (48.99%), Query Frame = 0

Query:  156 TSPQDTDRSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVP-PAFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRD---------REP--------------------------------------------LAVREGDGERTAAVRA-GWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACA-HVDSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAAAEL---FVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVCLNKEKRR---VVSVMMHGKHHRPMRILGGEGTTGKDGPNLRGRVVYLK-EEDCLAFRAAHALFP 1078
            T  Q+  R YR L L N ++ +LVSDP TDKS+AA+ V VG   DP E  GLAHFCEHMLFLGT+KYP EN Y   + + GGS+NA T MD T+Y F       +  L+ FAQFF+ PLFTESAT RE+ AV++EN KN+++D  R+ Q+   +    HP++ F TG+  TLK  +     ++RD LL+F+ + Y A  M LA++ ++SLD LE  V + F  +++         R P                                            L   EG G   +A++  GW++ + AG  TS   F  F ++V LTEEGL H  D+  LIFQY++++ R  P++ +   DE R I    FRF+ K S  SY +++   LQY+    VL A ++               ++E K  L     DN    F   +  LS   +   A+    EPWY   Y+ E  P+   +EW  A       LP  N F+  +F +    Y+   +    P ++  +    VW K D  +  P+A+++ +F +PL   DP    L   FV+     L E  Y   +AGL W + +   GL L   G++ K   LL K++E + T  +    F + +E  I   +N +  +P +H   +L++LLTE  W+ +E L  ++ +  + L  F  +++++  +   V GN S++ AL + ++VE  ++R+    PL P     +R + L+ G  +       N    +S +  Y+Q GL +     +L L  Q+++EPCF  LRT+EQLGYIV SGV+     + V GL   V S+ H P  +  R+E FL      I    S S   RH+EAL  + LE PK++    +  W EI +    + R +     +R   + D+++ +    +++ KRR    V V+   +        GG G    DG N     V  +  +D  AF+++H ++P
Sbjct:   53 TKSQEDKRVYRGLELNNGMKILLVSDPHTDKSSAAMDVNVGFLSDPKEVKGLAHFCEHMLFLGTEKYPNENDYSKFLQEHGGSTNATTYMDHTMYYFDVIPSNLSDALERFAQFFIAPLFTESATDREINAVNSENDKNISSDVWRLNQLENHLCDSEHPFNTFGTGNTYTLKTALKEKGINVRDELLKFHDKWYSANIMCLAVLGKESLDELEAMVVELFALVKNKQVVADSWERHPFRDDQLGSQVYVTPVKDVRNLNLVFPCGDYIQYYKSAPPQYISHLIGHEGPGSILSALKLRGWSNSLVAGQRTSLRGFSFFGISVDLTEEGLKHVNDIVKLIFQYINMMKREKPQQWV--QDENRDIGNMIFRFKDKESPRSYISNIVNSLQYYPLEDVLYANYM---------------LSEWKPDLIEEVWDN----FDPEKARLSVVAKAHAAICTETEPWYGTKYKEEKIPSETLKEWKNAGFCQEFRLPEQNEFIPTDFTL----YQQEDSESDNPVIIEDSALTRVWFKQDNKFLLPKANVMFDFVSPLAYLDPLNCNLTHMFVQLFRDELNEYAYAAELAGLKWELINTKYGLILGIGGYNSKQDILLKKIMEKLTTFKVDPKRFHIWKENYIRNLQNFSAEQPYQHAVYYLTVLLTEHSWSKEELLASIDQISVDRLEAFIPQMLSKMHIECLVHGNASKDRALEMVQIVESALKRSMNMSPLLPRQLLLNRELKLEDGCHYSYEK--ENKVHKSSCIEVYYQCGLQSRENNVKLELFCQLIQEPCFNVLRTKEQLGYIVFSGVRK---SNGVQGLRIIVQSERH-PSYLDGRIEEFLKSMQNYIRNKDSRS--FRHREALAAHRLEKPKQLAALTNQFWVEITSQQYHFNRAEDEVAYLRTLTKSDVLDFYQDQLMDEAKRRKKLAVRVISTAE--------GGAGVQNGDGKNSTTPAVQPQLIDDITAFKSSHEMYP 997          

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|908423375|ref|XP_013073073.1| (PREDICTED: insulin-degrading enzyme-like isoform X2 [Biomphalaria glabrata] >gi|908423377|ref|XP_013073075.1| PREDICTED: insulin-degrading enzyme-like isoform X2 [Biomphalaria glabrata])

HSP 1 Score: 483.026 bits (1242), Expect = 4.919e-150
Identity = 312/992 (31.45%), Postives = 505/992 (50.91%), Query Frame = 0

Query:  163 RSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVPPA--FDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEG-------HVRDKFGAI----------------------RDREPLAV------------------------REGDGERTAAVRA-GWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHV-DSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAAAEL---FVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIV---PNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSM-VAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELF-DSVCLNKEKRRVVSV-------MMHGKHHRPMRILGGEGTTGKDG----PNLRGRVVYLKEEDCLAFRAAHALFP 1078
            R YR L L N ++ +L+SDP TDKS+AA+ V +G  +DP + PGLAHFCEHMLFLGT+KYP EN Y   +N+ GG+SNAFT+++ T Y F  S D  AG LD FAQFF  PLFT SAT RE+ AVD+ENS+NL +D  R++Q+ K++A P H +SKF TG+ +TL E +P +   D+RD LL+F++++Y +  M L ++ ++SLD L+        HV +K   I                      +D   L+V                         EG G   + ++A GWA+ +  G       F  F V V L+EEG  H  D+  LI+QY++++ +  P++ +  + E + ++A +FRF+ K +  +Y + ++ +L     + VLSA  LFD  +   ++  L  +  E                    + +S   +    V D  E WY   Y+++ F     ++W+   + + L LP  N F+   FE++   ++P       P +V   P   +W+K D  + +P+A + ++F +P+   DP    L   F +  +  L E +Y   +AGL +S+++   GLTL   G++ K+  LL K+++ + T  +  +   + +E      +N    +P +H   + ++L +E  W+  E LQ L+ +  E+  +F  +++ R  +   V+GNV++  +L + E +E ++  + + +PL  S     R V L  G     C  V    N    +SS+  Y+Q G  T      L LL Q+M EPCF  LRT+EQLGYIV SGV+    RS  V GL   ++     P+ +  R+EAF+ +F   +L  +S  E  +H  AL T  LE PKK++ +   +W EI++    + R+Q+    +++  +DDL+  + + +  +  +R  +SV       M + +  R       E TTG DG    P+L    V     D + F+    L+P
Sbjct:   21 RQYRGLELKNGMKILLISDPETDKSSAAMDVHIGHLKDPSDLPGLAHFCEHMLFLGTEKYPEENEYNKFLNEHGGASNAFTTLEHTNYYFDISPDHLAGALDRFAQFFTSPLFTPSATSREVNAVDSENSRNLQSDPWRLFQLEKSLARPEHDFSKFGTGNKETL-ETIPKSKGIDIRDELLKFHSQYYSSNVMGLCVLGKESLDDLQKLVVPLFEHVVNKKVQIPRWDNPPYTQNELQCIVYAVPVKDIRELSVLWPTPDVSEFYRANPGHYLGHLLGHEGAGSLLSELKARGWANTLVGGQRAGAKGFSFFSVAVDLSEEGQAHIDDIIVLIYQYINMLRKEQPKQWI--FQECQDLAAMSFRFKDKETPKAYTSSISSKLHEFPMKDVLSAYSLFDEFKPQLINLILDQLLPE-------------------NMRISVVSKKFEDVADQTEKWYGTQYKVDKFTPKQLKKWSTYKLHEKLALPEKNEFIPTNFELVPTDHQPSEV----PEIVKTTPYSLLWYKQDNKFLKPKACVNIDFISPVAYMDPLHTNLNALFTQLFDDALTEYSYMAEIAGLKYSLNNSIYGLTLTVRGYNDKLPILLEKIVKKMATFKVDPSRLAIFKEIHGRNIKNFQAEQPHQHAVYYTNMLTSETHWSKDELLQTLDDVTPENQQNFINKVLERLYIEALVYGNVTKSQSLQMIENLENILVEHCKTKPLLASQHRKLREVQLPDG-----CYYVHQEKNLVHESSSIEVYYQCGPQTTESNMLLELLCQVMSEPCFDTLRTKEQLGYIVFSGVR----RSKGVQGLRI-IVQSNKAPQYVEGRIEAFIMKF-EAMLENMSEVEFNKHVMALSTKRLEKPKKLLQQNMKYWSEIISNYYNFDRDQIEVAYLQKLKKDDLLAFYKEKIAFDAPRRHKLSVHVLSGVSMNNEQSIRDSETGAVELTTGTDGFEPSPSLPLPQVI---TDIMEFKRDLGLYP 972          

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|642914575|ref|XP_971897.2| (PREDICTED: insulin-degrading enzyme [Tribolium castaneum])

HSP 1 Score: 474.167 bits (1219), Expect = 2.180e-146
Identity = 318/986 (32.25%), Postives = 467/986 (47.36%), Query Frame = 0

Query:  159 QDTDRSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVPPA---FDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDREPLAVR-----------------------------------------------------EGDGERTAAVRA-GWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHVD-SLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAAAEL---FVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVC-LNKEKRRVVSVMMHGKHHRPMRILGGEGTTGKDGPNL----RGRVVYLKEEDCLAFRAAHALFP 1078
            Q+  R YR L L N+++ +LVSDPTTDKSAAA+ V VG   DP +  GLAHFCEHMLFLGT KYP EN Y   +++ GGSSNA T  D T+Y F    D     LD F+QFF+ PLFTESAT RE+ AV++E+ KN+ ND  R  Q+ K +A P HP+  F TG+  TL  D  P     ++RD LL+F+ + Y +  M LA++ ++SLD LE  V   F  ++D+   A R                                                     EG G   + ++A GW++ + AG   +      F VTV LTEEG+ H  D+  LIFQY++++ R  P++ +   DE R I    FRF+ K S  SY A L   LQ +    VLS  +LF       +         EK  + +  K  ++                    LD VEPWY   Y++   P      W  + +     LP  N F+  +FE+    Y         P ++       VW K D ++  P+A+++ +F +PL   DP    L    V+     L E  Y   +AGL W + +   GL L   G+S+K    L KV+E +    I    F + +E  I   +N A  +P +H   +L+ LLTE  W  +E L   E L  + L  F  +++++  +   + GN ++E AL L ++VE  +       PL P     +R + L+ G ++     V N+    S +  Y+Q GL +     +L L AQI++EPCF  LRT+EQLGYIV SG++     + V GL   V S  H P  + +R+E FL       L  +S  E  RH+EAL    LE PK++  + ++ W EI +    + R  +    +R   ++D+I+ + S+   N + R+ +SV      H      GG G             +G VV     D   F+++H + P
Sbjct:   59 QEDKRLYRGLELANHMKVLLVSDPTTDKSAAAMDVNVGFMSDPRDVYGLAHFCEHMLFLGTKKYPNENDYNKYLSEHGGSSNAATYPDHTIYYFDIVPDELNNALDRFSQFFIAPLFTESATDREMNAVNSEHEKNIPNDVWRKDQLDKHLADPKHPYHTFGTGNRHTL--DTLPKEKNINVRDELLKFHDKWYSSNIMCLAVLGKESLDDLEQMVVKLFSEVKDKAIAAPRWEEHPFKDEHFGTCVYMYPIKDVRNLNIVFPCRDLQEYYKSSPSHYISHLMGHEGPGSILSTLKARGWSNNLVAGSRPAPRGLGFFGVTVDLTEEGIKHIDDIVELIFQYLNMLKRQGPQKWV--QDENRDIGNMLFRFKDKESPRSYIAGLVHTLQDYSMEDVLSCMYLFSEWRPDIIEQVWNDFVPEKIRIVVLAKQYENE-------------------LDQVEPWYGTKYKVAKIPEKTLERWRKSELSGDFKLPEKNEFIPTDFEL----YPIDKEVTEHPVIIQDTALTRVWFKQDETFLLPKANVMFDFVSPLAYLDPLNCNLTHMLVQLFRDALNEYAYAAELAGLKWELINTKYGLILAIGGYSNKQHIFLDKVMEKLTNFKIDPKRFEICKENYIRNLKNFAAEQPYQHAVYYLAALLTEHSWTKQELLATTEQLTIDKLEAFIPQILSKMHIECLIHGNANKEKALQLVQIVEDRLLSTLNMSPLLPRQLLLNRELKLEDGCNY--VYEVQNEVHKESCIELYYQCGLQSKENNMKLELFAQIVQEPCFDILRTKEQLGYIVFSGIRR---SNGVQGLRIIVQSDKH-PVRLDERIEEFLKNM-LSYLKNMSEEEFARHREALAAQRLEKPKQLSTQTNIFWGEITSQQYHFDRANVEVAYLRTLTKEDIIDFYKSLLEENAQFRKKLSV------HVVSMADGGAGKMAASDKEYTVDSKGTVVC----DITVFKSSHEMHP 1000          

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|242003176|ref|XP_002422640.1| (Insulin-degRading enzyme, putative [Pediculus humanus corporis] >gi|212505441|gb|EEB09902.1| Insulin-degRading enzyme, putative [Pediculus humanus corporis])

HSP 1 Score: 473.011 bits (1216), Expect = 7.729e-146
Identity = 314/999 (31.43%), Postives = 489/999 (48.95%), Query Frame = 0

Query:  153 GMETSPQDTDRSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTL----KEDVPPAFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDRE---------PLAVR--------------------------------------------EGDGERTAAVRA-GWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWA-CAHVDSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDP---AAAELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVA-GLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDS-VCLNKEKRRVVSVMM-------HGKHHRPMRILGGEGTTGKDGPNLRGRVVYLKEEDCLAFRAAHALFPCS 1080
            G+  SP+D  R YR L L N L+ +L+SD  T+K+AAAL+V VG   DP E PGLAHFCEHMLFLGT K+P EN Y   ++K GGS NA T+ D T Y F    +   G LD F+QFF+ PLF   AT RE++AV++E  KNL +D+ R  Q+ K ++  SHP+++F+ G+L+TL    KE+     D+R+ LL+F+ + Y A  M L ++ ++SLD LE   +  F  +++           P A                                              EG G   +A++  GW + + +G       F  + +   LT +G+ H  D+  L+FQY++++ +  P++ +  ++E++ +    FRF+ K     YAA L++ L  +    VL   +  +      ++  L ++  +                   R+ L A   +  A  D +EPW+   Y +E  P    ++W  C   ++L LP+PN F+   F I     E  SA+   PA++   P   VW+K D  +  P+A++  EF +PL   DP       LFV  L+ +LAE  YD  +AGL W I +   GL L  +G++ K   LL K+LE + T  I+   F+  +E  + A +N    +P      +LS+LL E  W   E L+  E L  E L+ F  +L+A+  +   + GNV+ +    + E V++ ++ +    P+ P     +R V L  G + +     P     +S   TY+Q  +++      + LL QI+K+PCF  LRT+EQLGY+V S VK    RS  A GL   V S  H P+ +  R+EAFL QF R ++  ++  E E HKE+L T LLE PKK+       W EIV+    + R+++    +R   ++DL+  FD  +    + RR +SV +        G    P  +       G         +   K ED   F+++H L+P +
Sbjct:   56 GIIKSPEDK-RCYRGLELDNKLKILLISDSDTEKAAAALTVHVG--NDPLELPGLAHFCEHMLFLGTKKFPVENDYSKFISKHGGSYNAVTAHDHTTYYFDVLPEHIEGALDRFSQFFLEPLFNADATEREIQAVNSEFEKNLPSDAWRFLQLDKHLSKESHPYNRFTIGNLKTLSTTPKEN---GIDIRNELLKFHDKWYSANLMTLVVLGKESLDDLEKLSKSLFTNVKNNNVEKPEWKEHPFATEHLQIKGYVVPVKDIRSIKICFPAPDYHEHYKSSPFNYISHLIGHEGPGSLLSALKERGWCNKLSSGYDNGIRGFAFYLIEADLTNDGMEHIDDILELVFQYLNMLKKEGPKQWI--FEEIQQLQKNKFRFKGKEGPIGYAATLSQLLPNYPMEEVLCGPYFLEEWNPDLINVALGYLEPKYC-----------------RIALIAQAYDKMA--DKIEPWFGAKYTVEKIPPSTIQKWENCGFDNALQLPKPNEFIPSNFNIYP--LEDESASSPHPAIIVDTPTTRVWYKQDDEFLLPKANLKFEFISPLAYLDPLNCTMTYLFVELLKDSLAEYDYDAAIAGLKWKILNTEYGLMLTIAGYNDKQVLLLDKILEKITTFKINANRFKYIKENYVRALKNFQAQQPYSQAAYYLSILLQEHAWTKDELLKSTEYLTIERLSEFIPQLLAKLHIEFLIHGNVNRDGVRKIIETVDKRLQCDSTLLPVLPRQLLRTREVQLVDGSNFKYETTNP--FFNSSCTETYYQCDVLSTKNNMLMELLIQIIKDPCFNILRTKEQLGYVVFSAVK----RSNCAQGLQIIVQSNRH-PKYVDQRIEAFLIQF-RNLVEEMTEKEFESHKESLATLLLEKPKKLSVLTLKFWAEIVSQQYHFNRSEVEVSHLRTITKNDLLAFFDQFIKYGADHRRKLSVYVLALGEGGAGNEPEPDEVALSSSQEGLPS--PPPFIPPSKIEDITKFKSSHGLYPLT 1015          

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|1126162422|ref|XP_019635746.1| (PREDICTED: insulin-degrading enzyme-like isoform X2 [Branchiostoma belcheri] >gi|1126162424|ref|XP_019635757.1| PREDICTED: insulin-degrading enzyme-like isoform X2 [Branchiostoma belcheri])

HSP 1 Score: 469.929 bits (1208), Expect = 4.952e-145
Identity = 310/946 (32.77%), Postives = 468/946 (49.47%), Query Frame = 0

Query:  163 RSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVPPA---FDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDR--------------EPLAVR---------------------------------------EGDGERTAAVRA-GWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHVD-SLHLPRPNPFVAEEFEILAPSYEP-PSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAA---AELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELF-DSVCLNKEKRRVVSVMMHGKHHRPMRILGGE 1045
            R+YR L L N ++ +LVSDPTTDK+AAAL V +G   DP   PGLAHFCEHMLFLGT KYP EN Y   +N+ GG+SNAFT+ + T Y F  S+    G LD FAQFF+ PLF E +  RE+ AVD+EN KNL +D  R+ Q+ KA A   HP+SKF TG+  TL E   P+    D+R+ LL++++ +Y A  MAL+++ ++ LD L   V DKF ++ ++              E L +R                                       EG G   + ++A GW + +  G     + F  F V V LTEEGL H  D+ + IFQY+ ++ +   +E +  ++E R +SA  FRF+ K    +Y + LA  LQ +   HVL A +L +  E   +   L+ +T EK  + +  K  +                      D  E WY+  Y++E       ++W+       L LP PN F+  +FEI     +P P   PS P  +  +    VW K D ++  P+A +  EF  P+   DP     A +FV+     L E  Y   +AG+ +++ +   G  L   G+  K   LL KVL+ + +  I    F + +E    A  N    +P +H   + +LLL+E  W  +E +  L+ +  E+L  F ++L +   +   V GN++++ AL    LV   ++ N +  PL  S +   R V L  GV H+      N   + S+L TY+Q GL +      L LL QI+ EPCF QLRT+EQLGYIV SGV+     + V GL F +L     P  + +R+E F+ +     L  LS  E ++H  AL    L+ PKK+  E + HW EI+     + R+ +    ++   +++L+  + +       KRR +++ +     +P  + G E
Sbjct:   24 RTYRGLELANGMKVMLVSDPTTDKAAAALDVNIGYMCDPDNIPGLAHFCEHMLFLGTTKYPSENEYNRFLNEHGGASNAFTAAEHTNYYFDVSSQHLEGALDRFAQFFISPLFNEESKDREMNAVDSENEKNLKSDMWRLHQLEKATADQKHPFSKFGTGNKYTLSER--PSQLNLDVREELLKYHSTYYSANVMALSVLGKEDLDSLSRLVVDKFASVENKNVNVPEFPIHPFQEEHLRLRCDVVPVKDIRTMNVAFPLPDLHPYYRTNPGHYLGHLIGHEGPGSLLSLLKAKGWVNSLVGGQKEGGNGFMFFTVNVDLTEEGLGHVYDIVAHIFQYLEMLRQTGHQEWI--FNECRDLSALKFRFKDKEMPRNYTSHLAGLLQKYPMDHVLVAPYLCEDYEPQLIEDVLSKLTPEKVRVAVVAKAFE-------------------GATDSEEKWYKAQYRIEPISPDLIQQWSQVQTHPDLKLPPPNEFIPTQFEI-----KPRPDEAPSIPECIKDDAMCKVWFKQDDTFLLPKACLSFEFINPVAYADPLNCNLAYMFVQLYRDALTEYAYSAELAGVSYTLHNTTYGFYLAIGGYDDKQSILLKKVLDKMTSFTIDPKRFTIIKELYTRALENFKAEQPYQHAVYYTTLLLSEMAWTKEELVGSLDEVTVENLQSFVKQLFSHMYMECLVHGNLTQQEALDTVGLVVGSLQENTQTRPLLASQRVKHREVQLPQGVAHKYER--ENTVHSTSALETYYQCGLQSTRDNMLLELLCQILNEPCFNQLRTQEQLGYIVFSGVRRA---NSVQGLRF-ILQSDRQPAYLDERVEVFVQKMESH-LQDLSEEEFQKHVAALSVRRLDKPKKLTSETARHWGEILAQQYNFDRDNIEVAFLKTITKEELLNFYKEHFSWGAPKRRKLTIYV-----KPTEVAGTE 929          

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|1022767442|gb|KZS12273.1| (Uncharacterized protein APZ42_022325 [Daphnia magna])

HSP 1 Score: 470.7 bits (1210), Expect = 1.454e-144
Identity = 322/1057 (30.46%), Postives = 506/1057 (47.87%), Query Frame = 0

Query:   91 FQKTKSNSSLAMMSLTGSLTRIGSNSGSNSSLLRSLHSSSRRWTSLIRSMEHLWDREGGQGMGMETSPQDTDRSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVPP--AFDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDR--------------EPLAVR---------------------------------------EGDGERTAAVRA-GWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHV-DSLHLPRPNPFVAEEFEILAPSYEPPSATPSPPALVFANPCV-HVWHKVDLSYKQPRAHIVLEFATPLPQRDP---AAAELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELFDSVCLNKEKRR------VVSVMMHGKHHRPMRILGGEGTTGKDGPNLRGRVVYLKEEDCLAFRAAHALFPCS 1080
            F +     +L    L G+LT +   S S+  +    + S +RW ++++S                   +   R YR L L N ++ +L+SDP+TDKSAAA+ V VG   DP + PGLAHFCEHMLFLGT+KYP EN Y   +++ GGSSNAFT+ D T Y F       +  LD FAQFF+ PLFTESAT RE+ AVD+E+ KN+ +D+ R+ Q+ K+ + P+HP+SKF TG+  TL + +P      +R+ LL+F+ + Y A  M+L ++ ++SLD LE      F  + ++              E L V+                                       EG G   + +++ GW + + AG  +    F  F + V LTE+G+ H  D+ +L FQY++++ +  P++ +  +DEL  +S   FRF+ K    SY   LA +LQY+    V+S  + F   +   +++ L  +T E   + +  K  +S                   V D  E WY   Y+LE   +     W  A + + LH+P  N F+ E+ +++          P P  ++  N  +  VW K D  +  P+A + +E  +P+   DP   +   L        L E TY   VAGLG+++ S   GL L   G++ K+ TLL K++E + T  +    FR+ +E  + A +N    +P +H     ++LL E  W+  + L   + L  E L  F   L ++  +     GN++++ A+ + + VE  ++ +   +PL P      R V ++ G +   CA   ND      + TY Q GL        L L  QI+KEPCF  LRT+EQLGYIV SGV+       V GL F V S+   P  +  R+EAFL     + L  +S  E ERHK AL     E PK++   A  +W EI  G   + R+ +  + + +    +L++ F S   ++   R      +++  +  +   P+    G  T  +  P +R   +    ED  AF+ +  LFP +
Sbjct:   54 FARISCKVNLRSKILFGNLTLLTRQSSSSPIVKMDGNHSYKRWDNIVKS-------------------EQDKREYRGLMLKNEMKILLISDPSTDKSAAAMEVNVGHMCDPHDLPGLAHFCEHMLFLGTEKYPVENEYPRFLSEHGGSSNAFTASDHTNYYFDVVPLQLSAALDRFAQFFLTPLFTESATDREVNAVDSEHVKNIPSDAWRLSQLDKSTSNPNHPYSKFGTGNKDTL-DTIPKERGIQVREELLKFHKKWYSANLMSLVVLGQESLDELEKLCVGLFSEVENKNVESPEWKEHPFGPENLQVKGLVVPVKDIRNLNITFPVPDMREHYETQPERYLSHLIGHEGPGSLLSELKSRGWVNSLMAGESSGAKGFAFFGINVDLTEDGIEHVDDIVTLAFQYLNMLRKLEPQKWV--FDELEGLSRVQFRFKDKEKPQSYVCSLASKLQYYPMEEVISGDYSFKEWKPDLVTSVLDMLTPENIRIAVIGKKFES-------------------VADSKEVWYGTSYKLEKIDSKDIEAWKNAGLSEKLHMPHRNEFIPEKLDLVT------REEPQPWPIILKNSQLSRVWFKQDTEFLLPKAVVYIEMFSPIAYLDPLRCSQVCLLASLFHDALNEFTYAAEVAGLGYALQSTKYGLQLSLKGYNDKLPTLLQKLIEKLTTFTVDPQRFRILKESYVRALQNFRAEQPYQHATYHTNMLLAERAWSKTDLLNSTDDLTVESLQSFIPFLFSQLHLEFLFHGNLTKQQAMDMVDTVESGLKTHFATKPLLPCQLIRDREVQMNNGANFLYCA--DNDVHATHCVETYLQLGLEDKRSNMLLELAMQILKEPCFNVLRTQEQLGYIVFSGVRRA---HGVQGLRFIVQSEK-PPTYVDGRIEAFLHSM-EQTLKDMSAEEYERHKTALAVRRQEKPKQLSHRAVRYWSEITTGQYFFDRDDMEVEELMQITHQELLDFFASYVFHQSPLRRKMAVHILASNVSQEKAEPVVHTNGGVTLSQPPPQIRETELV---EDVAAFKKSMPLFPLA 1053          

BLAST of EWM25916.1 vs. NCBI_GenBank
Match: gi|1126162420|ref|XP_019635736.1| (PREDICTED: insulin-degrading enzyme-like isoform X1 [Branchiostoma belcheri])

HSP 1 Score: 469.544 bits (1207), Expect = 1.648e-144
Identity = 310/946 (32.77%), Postives = 468/946 (49.47%), Query Frame = 0

Query:  163 RSYRLLRLPNNLRAILVSDPTTDKSAAALSVRVGASQDPPEAPGLAHFCEHMLFLGTDKYPRENTYKDLVNKSGGSSNAFTSMDLTLYKFQASADAFAGVLDVFAQFFMCPLFTESATGRELKAVDAENSKNLTNDSRRMWQITKAVATPSHPWSKFSTGSLQTLKEDVPPA---FDLRDALLRFYAEHYYAGNMALALVSRDSLDVLEGHVRDKFGAIRDR--------------EPLAVR---------------------------------------EGDGERTAAVRA-GWASGVHAGLGTSQDDFCLFEVTVALTEEGLPHWQDVASLIFQYVHLIGRASPEELLVLWDELRVISATAFRFQQKSSEYSYAADLARRLQYHQDRHVLSAGHLFDPLEEASLSACLAHMTEEKAILTLSCKDNQSRFFHRHRLDLSAAPRNSPAVLDLVEPWYQIPYQLELFPAHATREWACAHVD-SLHLPRPNPFVAEEFEILAPSYEP-PSATPSPPALVFANPCVHVWHKVDLSYKQPRAHIVLEFATPLPQRDPAA---AELFVRYLEHTLAESTYDGVVAGLGWSISSHARGLTLRFSGFSHKVQTLLHKVLESVLTTDIHDALFRLSREKAIDAYRNIALARPDEHGQMFLSLLLTEGRWAWKEKLQRLESLQAEDLAHFHRELMARNSVTLGVFGNVSEESALGLGELVERLMRRNGRFEPLCPSLQPFSRAVMLDAGVDHRLCAIVPNDADTNSSLSTYFQAGLVTAAQTAQLMLLAQIMKEPCFTQLRTREQLGYIVGSGVKSMWFRSMVAGLSFRVLSKTHGPEDILDRLEAFLGQFHREILTVLSLSELERHKEALITNLLEPPKKMVGEASMHWEEIVNGTLEWKRNQLYADGVREAGRDDLIELF-DSVCLNKEKRRVVSVMMHGKHHRPMRILGGE 1045
            R+YR L L N ++ +LVSDPTTDK+AAAL V +G   DP   PGLAHFCEHMLFLGT KYP EN Y   +N+ GG+SNAFT+ + T Y F  S+    G LD FAQFF+ PLF E +  RE+ AVD+EN KNL +D  R+ Q+ KA A   HP+SKF TG+  TL E   P+    D+R+ LL++++ +Y A  MAL+++ ++ LD L   V DKF ++ ++              E L +R                                       EG G   + ++A GW + +  G     + F  F V V LTEEGL H  D+ + IFQY+ ++ +   +E +  ++E R +SA  FRF+ K    +Y + LA  LQ +   HVL A +L +  E   +   L+ +T EK  + +  K  +                      D  E WY+  Y++E       ++W+       L LP PN F+  +FEI     +P P   PS P  +  +    VW K D ++  P+A +  EF  P+   DP     A +FV+     L E  Y   +AG+ +++ +   G  L   G+  K   LL KVL+ + +  I    F + +E    A  N    +P +H   + +LLL+E  W  +E +  L+ +  E+L  F ++L +   +   V GN++++ AL    LV   ++ N +  PL  S +   R V L  GV H+      N   + S+L TY+Q GL +      L LL QI+ EPCF QLRT+EQLGYIV SGV+     + V GL F +L     P  + +R+E F+ +     L  LS  E ++H  AL    L+ PKK+  E + HW EI+     + R+ +    ++   +++L+  + +       KRR +++ +     +P  + G E
Sbjct:   66 RTYRGLELANGMKVMLVSDPTTDKAAAALDVNIGYMCDPDNIPGLAHFCEHMLFLGTTKYPSENEYNRFLNEHGGASNAFTAAEHTNYYFDVSSQHLEGALDRFAQFFISPLFNEESKDREMNAVDSENEKNLKSDMWRLHQLEKATADQKHPFSKFGTGNKYTLSER--PSQLNLDVREELLKYHSTYYSANVMALSVLGKEDLDSLSRLVVDKFASVENKNVNVPEFPIHPFQEEHLRLRCDVVPVKDIRTMNVAFPLPDLHPYYRTNPGHYLGHLIGHEGPGSLLSLLKAKGWVNSLVGGQKEGGNGFMFFTVNVDLTEEGLGHVYDIVAHIFQYLEMLRQTGHQEWI--FNECRDLSALKFRFKDKEMPRNYTSHLAGLLQKYPMDHVLVAPYLCEDYEPQLIEDVLSKLTPEKVRVAVVAKAFE-------------------GATDSEEKWYKAQYRIEPISPDLIQQWSQVQTHPDLKLPPPNEFIPTQFEI-----KPRPDEAPSIPECIKDDAMCKVWFKQDDTFLLPKACLSFEFINPVAYADPLNCNLAYMFVQLYRDALTEYAYSAELAGVSYTLHNTTYGFYLAIGGYDDKQSILLKKVLDKMTSFTIDPKRFTIIKELYTRALENFKAEQPYQHAVYYTTLLLSEMAWTKEELVGSLDEVTVENLQSFVKQLFSHMYMECLVHGNLTQQEALDTVGLVVGSLQENTQTRPLLASQRVKHREVQLPQGVAHKYER--ENTVHSTSALETYYQCGLQSTRDNMLLELLCQILNEPCFNQLRTQEQLGYIVFSGVRRA---NSVQGLRF-ILQSDRQPAYLDERVEVFVQKMESH-LQDLSEEEFQKHVAALSVRRLDKPKKLTSETARHWGEILAQQYNFDRDNIEVAFLKTITKEELLNFYKEHFSWGAPKRRKLTIYV-----KPTEVAGTE 971