EWM26091.1, cds4517 (CDS) Nannochloropsis gaditana

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|585108085|gb|EWM26091.1| (2-succinyl-6-hydroxy- -cyclohexadiene-1-carboxylate synthase [Nannochloropsis gaditana])

HSP 1 Score: 4282.25 bits (11105), Expect = 0.000e+0
Identity = 2121/2121 (100.00%), Postives = 2121/2121 (100.00%), Query Frame = 0

Query:    1 MVAALDERYVAGSTKNCGMTNSSNSSGFKTGSGNRRLHFHSSSSPEREGMTTAGSKDEKAMEVDTASCSCPSSSCSSPITSMSTPPPIPYFGLPLSGHHRIGGYHAFPVPLGKARDLLMDAVRDMAIPSLHSPLSPPASRSSNISEKAPCTTPAAESSSAPPRFVRIEVPVSDILDEESALLWLRGQPERLMPRLFFRVPERGLVVAGVGAAHCVGGAEELGASKEKLGLCGKDVPAAVTYYGGGRFDPQYPHPSAEWQGFGASYLVLPALELIRRTRHGDGPLDGSFLLALNLRFSNVQELQAGRTALLHLLRDVQVAATDGDDEGAGDALLPAPVSCEEPSQFDAWHEGVGKALAAMQAGEYRKVVLARAVHFTFPRKPRPLDLVLRLRGHYGYLFCLQLDADRAFLGCTPEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHKDRFENRVTAESIEDALRPLVQGGRVERFPAFVLRLQHVQHICHKITGRLRGRSGAASESDTQEDWRESPETCSSSSAFDFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRSVLIVRERAHVFAGAGIVPGSTAEGEWAEIGAKMANFVRMFPRPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYTCFPRLRPSCPLSSDLLAAIHDAQRGLLVIGRLPQRRDRAHVRWLAQGLRWPIFADMGSGLRCDPALKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVASRAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTAGAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHADVFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIEVVTDRAQNVRIHRQLGAQIRTTVHAGLSQLLRLDWIRTGPRTKPTLLLLHGFLGCKEDWTPLLWEAGGCASASNAGLSCSSSSPNTIVGVSGGSGGRGLAMDYDCLAVELPGHGTTAMHGAGVGCSAAYSMPVVAFSLIQLLDRLGIAQCAVMGYSLGGRLAMYLAQQYPQRISAMIIVSAHPGLRAEGERLDRREEERRLGRRIEEAAHEGGEAWSAFLRWWYSKPLWGRLAERSPSYARMMRRRRAALARSEDPGGLSRAFAGMSLAQQPALWDFLRSGHVPTLIVYGANDVKYAGLGEELRASLVDGVGSSGGVTRTVVEVKRIDDAGHAVLVEKEMEMLRETTAFLRRHLEDAQERESGKGSAGGGSGVSKSKGGPSASPASLAATSSSSSSLQAQPMRVASLQWTTCSVALKKPLVLSSGPPLTHRRVIILVLEAVSGSVGVGEASPLPGFSKESFLEAEKQVKKISVALRGRTLPKLGGSKGLAAWKNWLESDVSLLPSVRAGLEMALVHLLAQEYRVDIPGVLKAGYPTHLLHTGQVCLNGLVLRTSPSLPSVPPSSLMSPTPSMPSSASFTASMDTSTSSSSKIKVGGRPVKEDVARVNNLTAELTFGHRLRLDANQAWSADEAVSFIRGLSHPEKIEYLEEPCKDPLCIPEVHKQTEQRLRFALDESLACPPLPYIELLSMEGLCALVLKPTVLGGLKQCLALHSLGLTKGLYSVVSSSFESSIGLAHLALLAAVLNSGTDTVRQIQTAGVHVLHGLSTYDVFVRSAEEWWGNGGGFSRLVASGSRSVLVDVVGCEELLDGFLKSIKHNDGRWKCENSIHAGVEKEEAIGPERAGREVCDGKRNDSP 2121
            MVAALDERYVAGSTKNCGMTNSSNSSGFKTGSGNRRLHFHSSSSPEREGMTTAGSKDEKAMEVDTASCSCPSSSCSSPITSMSTPPPIPYFGLPLSGHHRIGGYHAFPVPLGKARDLLMDAVRDMAIPSLHSPLSPPASRSSNISEKAPCTTPAAESSSAPPRFVRIEVPVSDILDEESALLWLRGQPERLMPRLFFRVPERGLVVAGVGAAHCVGGAEELGASKEKLGLCGKDVPAAVTYYGGGRFDPQYPHPSAEWQGFGASYLVLPALELIRRTRHGDGPLDGSFLLALNLRFSNVQELQAGRTALLHLLRDVQVAATDGDDEGAGDALLPAPVSCEEPSQFDAWHEGVGKALAAMQAGEYRKVVLARAVHFTFPRKPRPLDLVLRLRGHYGYLFCLQLDADRAFLGCTPEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHKDRFENRVTAESIEDALRPLVQGGRVERFPAFVLRLQHVQHICHKITGRLRGRSGAASESDTQEDWRESPETCSSSSAFDFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRSVLIVRERAHVFAGAGIVPGSTAEGEWAEIGAKMANFVRMFPRPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYTCFPRLRPSCPLSSDLLAAIHDAQRGLLVIGRLPQRRDRAHVRWLAQGLRWPIFADMGSGLRCDPALKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVASRAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTAGAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHADVFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIEVVTDRAQNVRIHRQLGAQIRTTVHAGLSQLLRLDWIRTGPRTKPTLLLLHGFLGCKEDWTPLLWEAGGCASASNAGLSCSSSSPNTIVGVSGGSGGRGLAMDYDCLAVELPGHGTTAMHGAGVGCSAAYSMPVVAFSLIQLLDRLGIAQCAVMGYSLGGRLAMYLAQQYPQRISAMIIVSAHPGLRAEGERLDRREEERRLGRRIEEAAHEGGEAWSAFLRWWYSKPLWGRLAERSPSYARMMRRRRAALARSEDPGGLSRAFAGMSLAQQPALWDFLRSGHVPTLIVYGANDVKYAGLGEELRASLVDGVGSSGGVTRTVVEVKRIDDAGHAVLVEKEMEMLRETTAFLRRHLEDAQERESGKGSAGGGSGVSKSKGGPSASPASLAATSSSSSSLQAQPMRVASLQWTTCSVALKKPLVLSSGPPLTHRRVIILVLEAVSGSVGVGEASPLPGFSKESFLEAEKQVKKISVALRGRTLPKLGGSKGLAAWKNWLESDVSLLPSVRAGLEMALVHLLAQEYRVDIPGVLKAGYPTHLLHTGQVCLNGLVLRTSPSLPSVPPSSLMSPTPSMPSSASFTASMDTSTSSSSKIKVGGRPVKEDVARVNNLTAELTFGHRLRLDANQAWSADEAVSFIRGLSHPEKIEYLEEPCKDPLCIPEVHKQTEQRLRFALDESLACPPLPYIELLSMEGLCALVLKPTVLGGLKQCLALHSLGLTKGLYSVVSSSFESSIGLAHLALLAAVLNSGTDTVRQIQTAGVHVLHGLSTYDVFVRSAEEWWGNGGGFSRLVASGSRSVLVDVVGCEELLDGFLKSIKHNDGRWKCENSIHAGVEKEEAIGPERAGREVCDGKRNDSP
Sbjct:    1 MVAALDERYVAGSTKNCGMTNSSNSSGFKTGSGNRRLHFHSSSSPEREGMTTAGSKDEKAMEVDTASCSCPSSSCSSPITSMSTPPPIPYFGLPLSGHHRIGGYHAFPVPLGKARDLLMDAVRDMAIPSLHSPLSPPASRSSNISEKAPCTTPAAESSSAPPRFVRIEVPVSDILDEESALLWLRGQPERLMPRLFFRVPERGLVVAGVGAAHCVGGAEELGASKEKLGLCGKDVPAAVTYYGGGRFDPQYPHPSAEWQGFGASYLVLPALELIRRTRHGDGPLDGSFLLALNLRFSNVQELQAGRTALLHLLRDVQVAATDGDDEGAGDALLPAPVSCEEPSQFDAWHEGVGKALAAMQAGEYRKVVLARAVHFTFPRKPRPLDLVLRLRGHYGYLFCLQLDADRAFLGCTPEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHKDRFENRVTAESIEDALRPLVQGGRVERFPAFVLRLQHVQHICHKITGRLRGRSGAASESDTQEDWRESPETCSSSSAFDFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRSVLIVRERAHVFAGAGIVPGSTAEGEWAEIGAKMANFVRMFPRPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYTCFPRLRPSCPLSSDLLAAIHDAQRGLLVIGRLPQRRDRAHVRWLAQGLRWPIFADMGSGLRCDPALKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVASRAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTAGAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHADVFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIEVVTDRAQNVRIHRQLGAQIRTTVHAGLSQLLRLDWIRTGPRTKPTLLLLHGFLGCKEDWTPLLWEAGGCASASNAGLSCSSSSPNTIVGVSGGSGGRGLAMDYDCLAVELPGHGTTAMHGAGVGCSAAYSMPVVAFSLIQLLDRLGIAQCAVMGYSLGGRLAMYLAQQYPQRISAMIIVSAHPGLRAEGERLDRREEERRLGRRIEEAAHEGGEAWSAFLRWWYSKPLWGRLAERSPSYARMMRRRRAALARSEDPGGLSRAFAGMSLAQQPALWDFLRSGHVPTLIVYGANDVKYAGLGEELRASLVDGVGSSGGVTRTVVEVKRIDDAGHAVLVEKEMEMLRETTAFLRRHLEDAQERESGKGSAGGGSGVSKSKGGPSASPASLAATSSSSSSLQAQPMRVASLQWTTCSVALKKPLVLSSGPPLTHRRVIILVLEAVSGSVGVGEASPLPGFSKESFLEAEKQVKKISVALRGRTLPKLGGSKGLAAWKNWLESDVSLLPSVRAGLEMALVHLLAQEYRVDIPGVLKAGYPTHLLHTGQVCLNGLVLRTSPSLPSVPPSSLMSPTPSMPSSASFTASMDTSTSSSSKIKVGGRPVKEDVARVNNLTAELTFGHRLRLDANQAWSADEAVSFIRGLSHPEKIEYLEEPCKDPLCIPEVHKQTEQRLRFALDESLACPPLPYIELLSMEGLCALVLKPTVLGGLKQCLALHSLGLTKGLYSVVSSSFESSIGLAHLALLAAVLNSGTDTVRQIQTAGVHVLHGLSTYDVFVRSAEEWWGNGGGFSRLVASGSRSVLVDVVGCEELLDGFLKSIKHNDGRWKCENSIHAGVEKEEAIGPERAGREVCDGKRNDSP 2121          

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|873219997|emb|CEM36643.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 775.778 bits (2002), Expect = 0.000e+0
Identity = 615/1680 (36.61%), Postives = 856/1680 (50.95%), Query Frame = 0

Query:  486 VLRLQHVQHICHKITGRLRGRSGAASESDTQEDWRESPETCSSSSAF-----DFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRSVLIVRERA--------HVFAGAGIVPGSTAEGEWAEIGAKMANFVRMFPRPSPPL----RSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVH--HDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVS---MARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGG-----RRFAQWERSLQPYTCFPRLR---PSCPLSSDLLAAIHDAQRGLLVIGRLPQRR-DRAHVRWLAQGLRWPIFADMGSGLR-------CDPALKHLLVAPYESLLAD-PLISSALDPDVILQIGSPLVSKRLPKFM------VASRAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEAD--TLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATL-ASTAGAP----SAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHADVFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIE-VVTDRAQNVRIHRQLGAQIRTTVHAGLSQLLRLDWIRTGPRTKPTLLLLHGFLGCKEDWTPLLWEAGGCASASNAGLSCSSSSPNTIVGVSGGSGGRGLAMDYDCLAVELPGHGTTAMHGAGVGCSAAYSMPVVAFSLIQLLDRLGIAQCAVMGYSLGGRLAMYLAQQYPQRISAMIIVSAHPGLRAEGERLDRREEERRLGRRIEEAAHEGGEAWSAFLR-WWYSKPLWGRLA---------ERSPSYARM--MRRRRAALARSEDPGGLSRAFAGMSLAQQPALWDFLRSGHVPTLIVYGANDVKYAGLGEELRASLVDGVGSSGGVTRTVVEVKRID----DAGHAVLVEKEMEMLRETTAFLRRHLEDAQERESGKGSAGGGSGVSKSKGGPSASPASLAATSSSSSSLQAQPMRVASLQWTTCSVALKKPLVLSSGPPLTHRRVIILVLEAVSGSVGVGEASPLPGFSKESFLEAEKQVKKISVALRGRTLP-KLGG---SKGLAAWKNWLESDVS-LLPSVRAGLEMALVHLLAQEYRVDIPGVLKAGYPTHLLHTGQVCLNGLVLRTSPSLPSVPPSSLMSPTPSMPSSASFTASMDTSTSSSSKIKVGGRPVKEDVARVNNLTAELT-----FGHRLRLDANQAWSADEAVSFIRGLSHPE--KIEYLEEPCKDPLCIPEVHKQTEQRLRFALDESLACPPLPYIELLSM--EGLCALVLKPTVLGGLKQCLALHSLGLTKGLYSVVSSSFESSIGLAHLALLAAVLNSGTDTVRQIQTAGVHVL-HGLSTYDVFVRSAEEWWGNGGGFSRLVASGSRSVLVDVVGCEELLDGFLKSIK 2081
            V +L+HVQH+   +  RL  +    S +   +    +     S+S         +  LHPT AV G P+  A  A+R+LE FDRGFYAGP+G+++   +EFAVAIRS LI  + +        HVFAGAGIVPGSTA GEW E   KM NFV +   P+ PL    RS PN+ T+    +VEE  R G++ F +APGSRS+P+VTA ARHP    +    HDERGA F  +GYARA GR A ++V+SGTAVANL P  VEA    +P++L TADRPPE R  G+ QT+ QPD+F  + RW KDV CPT  +  S LL+DVD AV+    A+G   GPV LN   RENLAP+GG VR T  PGL S W   CL G      R A+W++S  PY  +        S   +S++L+ +  ++RGL+V G    R  D     WL   L WP+  D+ S LR          A K   VA  + LLA  P I+  L  D++LQ+G PL SKR+   +       A+ AAT++ V P P   DP+   TH +   I +FV  LD            AR + +        + + H  A EL   AD  ++  AG   F          L           + S  L EP  A L A    +  GLFLS+SMP+RD ++F +L  + A AP    S   P+  VA NRGA+GIDG+L SA+GY+ GLG+PVT+L+GD+A LHD+  LH L  + AS S                 IF FLPIA H DVFSP FD+PHD+ F  I  + GLPY   K++ +L AA+    A  R  +IE V TDR  NV IHR +       V   L++ +R  W R G R  PT++ LHG+LG K+DW                           I  ++  +GG+ + +     AV+L GHG T     G+  S A++  VV  SL+ LL   GI    ++GYS+GGRLAM L ++ PQR+ A +I++A PG     ER +R  + + L  R+E        A+  FLR  WYS P+WG LA         ++ P+ A +  + RRR   +    P   + +  GMS A QP L   L     P L + G +D +Y  + EEL  S ++G              +R++    D+GHAV+ E+   + +   AFL++H   A +                         A+ AA ++    L +    + SL     ++ L +PL LS    +T RR  +++    +G+VG+GE  PLPGF  E+  EA   +  ++   +G  +P +LG    S+   AW   L+     ++PSVR GL+ A++HLLA+E    IP ++ +   +    +  V LNGL +R+  ++     SS                S         K+KVGGR   ED  RVN +  ++         R+RLDANQAW   EA +F R LS      IEY+EEP  D   +P  H+ T  R+ FALDESL        +L S+  +GL ALVLKP +LGG  +CL  HS+    G+ SV+S++FESS+ L H AL+A++L++ T +       G  V+ HGLST++     + +       FS  V       LVD+  C+ +LD   +S K
Sbjct:  502 VKQLKHVQHLFRTV--RLNLQQALRSYAPPTDVTDRADRGGQSNSGLLRLGVRLMHGLHPTAAVCGLPKGIAADAVRRLEDFDRGFYAGPVGYVAATGSEFAVAIRSALIRPDDSGRRPSSVVHVFAGAGIVPGSTASGEWRETAVKMRNFVEVLGPPAHPLALRLRSAPNINTMGGVAIVEEFLRLGISRFFVAPGSRSSPIVTALARHPLAARVTTSVHDERGAAFMGVGYARATGRAAVIVVSSGTAVANLYPGVVEAWQAGLPMVLCTADRPPELRGRGSMQTIWQPDIFGKYVRWMKDVSCPTDHIALSHLLADVDCAVAHAEGAKGGGKGPVQLNLPFRENLAPDGGPVRGT--PGLASTWSHGCLDGFGHGDERLAKWQQSCSPYAIYVPGEGGGMSRDAASEVLSLLSSSRRGLVVAGGGQWREGDVQAAIWLVGQLGWPVVTDITSSLRGPIDEHNKQAAEKIEHVAAADLLLAGCPFITHHLRTDLVLQLGIPLTSKRMYALIETAMKKTANEAATIV-VSPEPHCHDPDAVMTHQLTCSIASFVEALDDV----------ARPAAI--------SEVAH--ASELCGLADLTSIAIAGAANF----------LKAQEGESGDGSSSSAALCEPEVARLAASSAQSTGGLFLSSSMPIRDADSFGSLPDAIATAPDVGRSDEGPVGCVASNRGANGIDGVLHSAIGYSLGLGQPVTVLLGDVAALHDMGGLHTLKQSGASVSVVVVNNGGGG-------IFHFLPIAAHKDVFSPLFDTPHDVSFEGICASVGLPYSRVKTSEELSAAVSGKSAFPR--VIEAVCTDRESNVSIHRDIQRAAGDAVVTHLARSIRWCWWRGGCRDSPTVVFLHGWLGSKDDWR------------------------TVIASLTQQAGGQCVGL----FAVDLLGHGGTRSAAEGLAASLAHTPDVVTTSLLDLLASEGITDAILVGYSMGGRLAMQLMKKAPQRVRAAVIIAADPGATDPAERHERFCKGQVLAGRVEAMDR---CAFERFLRSEWYSMPMWGPLAAPCSPLEDSDQQPTTALLTDIIRRRCRTS----PRAAAASLRGMSAALQPDLRPTLVDPPCPALFIAGRHDARYHAISEELVESSIEG--------------RRLEVATADSGHAVVEEQPTWLAQAIGAFLQKHQLVAID------------------------DATAAAAAAPPPPLPSPAPVLDSLAIVPFTMDLSQPLQLSQFDAITERRGWLILARNAAGTVGIGEVCPLPGFHNETHGEAAALLTGLA-GQQGVDIPWQLGALRDSRAFDAWVTTLKGGRQPVVPSVRLGLQQAVLHLLARERGQAIPELVTS-MRSGTSRSSHVHLNGLRVRSHATIGGDASSS----------------SERCVRYPVVKVKVGGRSTAEDARRVNQMAEDVMADSDGVAPRIRLDANQAWLLREADAFFRQLSERSLALIEYVEEPLADCQLLPVFHRAT--RVHFALDESLYQEHFRLSDLPSLFGKGLAALVLKPALLGGYDKCLDFHSVASKAGVPSVISAAFESSVTLHHHALIASMLDTVTASGATSAPVGSDVVSHGLSTFETLASDSVQ----QCSFSCAV---DEEGLVDIRVCQAMLDRAAESAK 2037          

HSP 2 Score: 121.324 bits (303), Expect = 1.692e-23
Identity = 67/138 (48.55%), Postives = 83/138 (60.14%), Query Frame = 0

Query:  340 EEPSQFDAWHEGVGKALAAMQAGEYRKVVLARAVHFTFPRKPRPLDLVLRL----RGHYGYLFCLQLDADRAFLGCTPEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHKDRFENRVTAESIEDALRPL 473
            E    F+ W   +  AL  +  G+Y+K VLAR+V   FP    PLD++L+L    R   GYL CL+  A   F+GCTPEQL  V    +ST ALAGTRPRG T E+D+ALA ELM   KDR E  +T ESI+ AL  L
Sbjct:  284 ESGEAFERWTAAINTALQDITDGKYKKTVLARSVQLKFPDALEPLDILLQLYGSTRATTGYLLCLKPRASVGFVGCTPEQLLSVRQQQLSTVALAGTRPRGATKEQDDALARELMEDPKDRTELDLTLESIQVALTQL 421          

HSP 3 Score: 69.3218 bits (168), Expect = 8.911e-8
Identity = 40/106 (37.74%), Postives = 54/106 (50.94%), Query Frame = 0

Query:  174 ILDEESALLWLRGQPERLMPRLFFRVPERGLVVAGVGAAHCVGGAEELGASKEKLGLCGKDVPAAVTYYGGGRFDPQYPHPSAEWQGFGASYLVLPALELIRRTRH 279
            + +   A++WLR Q   L P+++F  P   LV AGVGAAH + G   L  S  ++     + P AV YYGG RFD        EW+ F   +  LP +EL R   H
Sbjct:   61 VQNARDAIIWLRNQS--LHPQVYFSNPSGDLVAAGVGAAHLITGNGRLAESDVQV-----EAPTAVHYYGGERFDVTAER-DKEWEAFQDHFFFLPLIELRRTAPH 158          

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|1210516047|dbj|GAX25132.1| (isochorismate synthase / 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase / 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / O-succinylbenzoate synthase [Fistulifera solaris])

HSP 1 Score: 622.468 bits (1604), Expect = 0.000e+0
Identity = 623/1996 (31.21%), Postives = 884/1996 (44.29%), Query Frame = 0

Query:  157 SSSAPPRFVRIEVPVSDILDEESALLWLRGQPERLMPRLFFRVPERGLVVAGVGAAHCVG-GAEELGASKEKLGLCGKDVPAAVTYYGGGRFDPQYPHPSAEWQGFGASYLVLPALELIRRTRHGDGPLDGSFLLALNLRFSNVQELQAGRTALLHLLRDVQVAATDGDDEGAGDALLPAPVSCEEPSQFDAWHEGVGKALAAMQA----GEY---RKVVLARAVHFTFPRKPRPLDLVLRLRGH--YGYLFCLQL-------------DADRAFLGCTPEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHKDRFENRVTAESIEDAL-RPLVQGGRVERFPAFVLRLQHVQHICHKITGRLRGRSGAASESDTQEDWRESPETCSSSSAF-DFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRS-VLIVRERAHVFAGAGIVPGSTAEGEWAEIGAKMANFVRMFPRPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAA----RHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYTC--FPRLRPSCPLSSDLLAAIHDAQRGLLVIGRL-PQRRDRAH--VRWLAQGLRWPIFADMGSGLRCDPALKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVASRAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRL------LNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTAGAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHADVFSPYFDSP-HDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIE-VVTDRAQNVRIHRQLGAQIRTTV--HAGLSQLLRLDWI----RTGPRTKPTLLLLHGFLGCKEDWTPLLWEAGGCASASNAGLSCSSSSPNTIVGVSGGSGGRGLAMDYDCLAVELPGHGTTAMHGAGVGCSAA---YSMPVVAFSLIQLL--DRLGIAQCAVMGYSLGGRLAMYLAQQY--PQRISAMIIVSAHPGLRAEGERLDRREEERRLGRRIEEAAHEGGEA------WSAFLRWWYSKPLWGRLAERSP-SYARMMRRRRAALARSEDPGGLSRAFAGMSLAQQPALWDFLRSGHVPTLIVYGANDVKYAGLGEELRASLVDGVGSSGGVTRTVVEVKRIDDAGHAVLVEKEMEMLRETTAFLRRHLEDAQERESGKGSAGGGSGVSKSKG-GPSASPASLAATSSSSSSLQAQP----------MRVASLQWTTCSVALKKPL-------VLSSGPPLTHRRVIILVLEAVSGSVGVGEASPLPGFSKESFLEAEKQVKKISVALRGRTLPKLGGSKGLAA----WKNWLESDVSLLPSVRAGLEMALVHLLAQEYRVDIPGVLKAGYPTHLLHTGQVCLNGLVLRTSPSLPSVPPSSLMSPTPSMPSSASFTASMDTSTSSSSKIKVGGRPVKEDVARVNNLTAELTFGHRLRLDANQAWSADEAVSF---IRG--LSHPEKIEYLEEPC-KDPLCIPEVHKQTEQRLRF--------ALDESLA---------------CPPLPYIELLSMEGLCALVLKPTVLGGLKQCLALHSLGLTK-GLYSVVSSSFESSIGLAHLALLAAVLNSGTDTVRQIQTAGVHVLHGLSTYDVF 2037
            SSS      R+EV  S  L     L WL+ Q +    + FF      L  A   A   V  G ++       L    + +P    +YG   FD       A+W        VLP LE  +   H            L L  +  +E +A R A         +     DD G+    LP PV  +     DA+ +GV +A+A MQ     GEY   +KVVLAR     F      +DL+ R +     G LF + L              +   F+GCTPEQLFR+    ++TEALAGTRPRG  P+ED  L  EL+ S KD  EN  TA  IE+ L R     G       FV R +H+QHIC                        ++P   ++S+   + L  LHPTPAV G P   A   IR++EPF R +YAGPIG++S    +  V IRS VL  ++   + AG+G+V  ST   E+AE  AK       F  P   L+S PN+ T WAT VVEE  R GV    I PGSRSTPL  A A    +HP   ++  HDER A F  +GY +A GRPA V+ +SGTAVANLLPA +EA  D  P+++LTADRP E RD  ANQ + Q  +F     W +DV  P+ EV   + LSD +YA  MA      PVH+N   RENLAPEGG VR      +          G  FA+W  S QP       R + +   ++DL+  + +++RGLLV+G++ P ++D  +  +  LAQ + +PI A   S LR      H +V   E LLA P +  AL PD ILQ+G+PL+S  +  ++  +    L+   P   R DPE S TH + +                           L   L+TL  R       EL        P G     S  SAL P LVP S   +P   +  L      L EP     +A+  P    LFLSNSMP+RD E+F       G   A  P+     NRG SGIDGI+++A G+A    +P  L++GD++ LHD N+        A ASAP   ++ ++VNN GG IFSFLP+A+H   F  +F +P   + +    +  G+ Y   K      A L+  +    ++L+E VV  R +NV++HR L  +I++ V  H+ +    R   +     T  +   TLLLLHG++GCKEDW  ++                       +V +           +++ ++++LPGH         +  + +   YS+  +A  +   L  D       AV GYSLGGR+ + L++ Y  PQ      + +    L        R   + +L ++++   H+   A      W  FL  WYS P+WG + ER P +Y  ++ RR   L R           AG S+AQ       + S   P  +     D  Y     + R  L+ G   S    ++   +  I ++GHA+L E    +      FL         R++ + SA     V K     P     ++  TSS +   +  P          + V SL +   S+ L             ++    T  R  +++   V   VG+GE SPL G   ES  EA+ Q++ +    R   +P +   + + A    W+ +L  DVS   SVR GLEMAL  LL         G+L +         G + LNGL  R+S           +SP               T    S KIKVG   +++D A ++      T G ++R DAN+AW  + A+ F   I G  L   +++EY+EEP  K       + +Q E   RF        ALDES+                     + E  S  G    VLKP VL G      + +L   +  +  V+SS  ES +GLAH A LA  +++  +    +        HGLSTY + 
Sbjct:   22 SSSENDTLYRLEVTFSRRL---QLLPWLKSQLQFDPEQHFFY-----LQTATEDAEEIVAWGIQQAVDHTSALWDDHRQLPPHARWYGAQTFDGDM----AQW--------VLPMLEWRQNATHT----------VLALHTTGTRE-EAARIA-------QSIKTRYLDDVGSISTPLP-PVVSKTDLAIDAYEQGVEEAIALMQQENQNGEYSDLQKVVLARQQLLQFKHPFHVVDLLHRWKQESGTGNLFAMALWDKKDDNTIITSTRSSSCFVGCTPEQLFRIHNCTVTTEALAGTRPRGSHPQEDADLLQELVNSPKDLDENACTARYIEEVLSRQQPSIGPFVTSQTFVRRTRHLQHICRTY---------------------QAPVWNNTSTLIRNLLEQLHPTPAVCGLPLNMARDYIRRVEPFSRDYYAGPIGYISPTECQLLVGIRSAVLRPKQELLLTAGSGLVLNSTLREEFAETVAKFQVVASWFSSPLS-LQSCPNINTAWATAVVEEFVRCGVDMVFICPGSRSTPLAVAWAWARRQHPFLKVISCHDERAAAFRAVGYGKASGRPAVVVTSSGTAVANLLPAVLEAHQDDSPMIVLTADRPYEHRDIAANQAMDQVKLFGDKVAWFRDVLPPSDEVNVVAALSDANYAHYMAL-EQQQPVHINVQFRENLAPEGGPVRGGDGDAVERYNATRFTQGPAFARWSGSGQPLIQRHSSRAQNNDVAAADLVQWLQNSRRGLLVVGQIPPSQQDEVYPLLDQLAQTIGFPIVA-TSSSLRFT---SHAVVWFAEHLLACPNVRDALQPDFILQVGAPLLSTTVTSYLKETAQHVLLQTNPR-RRVDPEYSVTHSIST-------------------------PNLAFFLETLVGR-----TSEL--------PKG-----SISSALTP-LVPWSRTLAPVMKEFLLQQSPDRLTEPQIILALAQQSPQ-QALFLSNSMPIRDAESFLY---PYGEIIASVPMTVTGSNRGVSGIDGIIATAQGFALNADEPTQLIIGDVSALHDSNSF-------AFASAPK--LSTLIVNNNGGAIFSFLPVAQHDVSFEEFFSTPTQHVDWEATARGLGVQY---KKVASYEALLNQWEQQNVNTLVEAVVISRDENVQLHRDLTRKIQSHVNEHSWMDYTTRPRILPLKQYTSNKANRTLLLLHGWMGCKEDWDDVV--------------------EKLMVHIGD---------NWNIVSIDLPGHSQVEPSIESIDSTTSGLNYSVSAMAEWIGDCLAQDYQLKDIHAVAGYSLGGRIGLELSRLYSIPQ------VTTISSNLEPVSADTGRAHRDDKLSQQLQRIFHQQLVATNPATLWGEFLESWYSAPIWGNMKERRPDTYRAILERRTEILQR-----------AGASIAQ-------VLSEASPGRLENHRCDECYF----DRRHCLLIGELDSKYALQSNATL--IPESGHALLWEAPTTVALHLAEFLS--CNGTLNRDAIQNSA--TISVEKPDAITPDPLDVNITTTSSDTERSKKLPIPTARQHEGHLIVDSLDYQIFSIPLLNDRNQGLGWGASATATSTTQNRTGMIIELRVFNYVGLGEVSPLKGVHAESLEEAQVQIQTLQEWFR-LNVPSVSLMEDIDALLDTWEQYL--DVSWHISVRCGLEMALRSLLWHA-----NGILPSS-------KGLLPLNGLQTRSS-----------LSP--------------QTQKWPSLKIKVGHDSLEQDQAAIHQ-GFRSTLG-KIRADANRAWDEEGALEFAAAIEGWELQARDRLEYIEEPLRKVNDATWSLEQQIEALERFYLQTSIPYALDESIGDLVQVHRRDWQEIREKLRTIFTEGRSRRGCACFVLKPAVL-GFALTTRIATLAREELQIPVVISSCMESGLGLAHAAFLAGEIDALPNNTPTLA-------HGLSTYSIL 1777          

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|1210524828|dbj|GAX16420.1| (isochorismate synthase / 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase / 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase / O-succinylbenzoate synthase [Fistulifera solaris])

HSP 1 Score: 601.668 bits (1550), Expect = 1.967e-176
Identity = 603/1940 (31.08%), Postives = 865/1940 (44.59%), Query Frame = 0

Query:  233 KDVPAAVTYYGGGRFDPQYPHPSAEWQGFGASYLVLPALELIRRTRHGDGPLDGSFLLALNLRFSNVQELQAGRTALLHLLRDVQVAATDGDDEGAGDALLPAPVSCEEPSQFDAWHEGVGKALAAMQA----GEY---RKVVLARAVHFTFPRKPRPLDLVLRLRGH--YGYLFCLQLDADR-------------AFLGCTPEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHKDRFENRVTAESIEDAL---RPLVQGGRVERFPAFVLRLQHVQHICHKITGRLRGRSGAASESDTQEDWRESPETCSSSSAF-DFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRS-VLIVRERAHVFAGAGIVPGSTAEGEWAEIGAKMANFVRMFPRPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAA----RHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYTCFPRLRPSCPLSSDLLAA-----IHDAQRGLLVIGRL-PQRRDRAH--VRWLAQGLRWPIFADMGSGLRCDPALKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVASRAATLIHVV-PSPARR--DPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTAGAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHADVFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIE-VVTDRAQNVRIHRQLGAQIRTTV--HAGLSQLLRLDWI----RTGPRTKPTLLLLHGFLGCKEDWTPLLWEAGGCASASNAGLSCSSSSPNTIVGVSGGSGGRGLAMDYDCLAVELPGHGTTAMHGAGVGCSAAYSMPVVAFSLIQLLDRLG--IAQ-------CAVMGYSLGGRLAMYLAQQYPQRISAMIIVSAHP---GLRAEGERLDRREEERRLGRRIEEAAHEGGEAWSAFLRWWYSKPLWGRLAERSP-SYARMMRRRRAALARSEDPGGLSRAFAGMSLAQ------------QPALWDFLRSGHVPTLIVYGANDVKYAGLGEELRASLVDGVGSSGGVTRTVVEVKRIDDAGHAVLVEKEMEMLRETTAFLRRHLEDAQERESGKGSAGGGSGVSKSKGGPSASPASLAATSSSS-----SSLQAQPMR-----VASLQWTTCSVALKKPLVLSSG--------PPLTHRRVIILVLEAVSGSVGVGEASPLPGFSKESFLEAEKQVKKISVALRGRTLPKLGGSKG----LAAWKNWLESDVSLLPSVRAGLEMALVHLLAQEYRVDIPGVLKAGYPTHLLHTGQVCLNGLVLRTSPSLPSVPPSSLMSPTPSMPSSASFTASMDTSTSSSSKIKVGGRPVKEDVARVNNLTAELTFGHRLRLDANQAWSADEAVSFIRGLSHPE-----KIEYLEEPCKDPLCIPEVHKQTEQRLR------------FALDESLACPPLPYIELL---------------SMEGLCALVLKPTVLGGLKQC----LALHSLGLTKGLYSVVSSSFESSIGLAHLALLAAVLNS---GTDTVRQIQTAGVHVLHGLSTYDVF 2037
            + +P    +YG   FD       A+W        VLP LE  +   H            L L  +  +E +A R A         +     DDE      LP PV  +     DA+ +GV +A+A MQ     GEY   +KVVLAR     F      +DL+ R +     G LF + L  ++              F+GCTPEQLFR+    ++TEALAGTRPRG  P+ED  L  EL+ S KD  EN  TA  IE+ L    P +  G       FV R +H+QHIC                        ++P   ++S+   + L  LHPTPAV G P   A   IR++EPF R +YAGPIG+LS   ++  V IRS VL  ++   + AG+G+V  ST   E+AE  AK       F  P   L+S PN+ T WAT VVEE  R GV    + PGSRSTPL  A A    +HP   ++  HDER A F  +GY +A GRPA V+ +SGTAVANLLPA +EA  D  P++LLTADRP E RD  ANQ + Q  +F     W +D+  P+ EV   + LSD ++A +MA      PVH+N   RENLAPEGG VR      + S        G  FA+W  S QP     +   S   ++D+ AA     + +++RGLLV+G++ P ++D  +  +  LAQ + +PI A   S LR      H +V   E LL+ P +  A+ PD ILQ+G+PL+S  +  ++      T  HV+  + ARR  DPE S TH + +  P   + L++ +     R  E  +  + S L  L             P + TL P            +   L+   SP        RL    +   L  + LP    LFLSNSMP+RD E+F       G      PL     NRG SGIDGI+++A G+A    +P  L++GD++ LHDLN+  A S A          ++ ++VNN GG IFSFLP+A+H   F  +F +P   H      A GL     K      A ++  +    ++L+E VV  R +NV++HR L  +I++ V  H  L    R   +     T      TLLLLHG++GCKEDW  ++ E                     +V V           D++ ++++LPGH     H      S + +   + +S+  + + +G  +AQ        AV GYSLGGR+ + L++ Y       I  S  P         +R D+  ++ +   + +  A      W  FL  WYS P+WG L ER P +Y  ++ RR A L R           AG S+AQ            +    D+    H    ++ G  D KY+             + S+  +         I ++GHA+L E    +      FL  +   ++  ++ + +A    G ++S    +    ++A TSS +     SS+     R     V SL +   S+ L K      G          + +R  +I+ L A +  VG+GE SPL G   ES  +A+ Q++ +   L+  + P +   +     L AW+  L    +   SVR GLEMAL  LL         G+L +         G + LNGL  R          +SL+  T   P               S K+KVG   ++ D A +       T G ++R DAN+AW  + A+ F   L   E     ++EY+EEP +    + E     EQ++             +ALDES+      +                   S  G    VLKP VLG         LA   L +      V+SS  ES +GLAH A LA  +++    T T+           HGLSTY + 
Sbjct:   81 RQLPLHARWYGAQTFDGNM----AQW--------VLPMLEWRQNVTHT----------VLALHTTGTRE-EAARIA-------QSINTRHLDDEWTIPTPLP-PVVSKTDLAIDAYEQGVEEAIALMQQEKQNGEYSDLQKVVLARQQLLQFKHPFHVVDLLQRWKQESGTGNLFAMALWDEKDDNTIITSTRSSSCFVGCTPEQLFRIHNSTVTTEALAGTRPRGSHPQEDADLLQELVNSPKDLDENACTARYIEEVLSKQEPSI--GPFVTSQTFVRRTRHLQHICRTY---------------------QAPVWNNTSTLIRNLLEQLHPTPAVCGLPLNMASDYIRRVEPFSRDYYAGPIGYLSSTESQLLVGIRSAVLRPKQELLLTAGSGLVLNSTLREEFAETVAKFQVVASWFSSPLS-LQSCPNINTAWATAVVEEFVRCGVDMVFVCPGSRSTPLAVAWAWARRQHPFLRVISCHDERAAAFRAVGYGKASGRPAVVVTSSGTAVANLLPAVLEAHQDDAPMILLTADRPSEHRDISANQAMDQVKLFGDKVAWFRDILPPSDEVNVVAALSDANFAHNMAL-EQQQPVHINVQFRENLAPEGGPVRGGDGDAVESYNATRFTQGPAFARWSGSGQPLI---QRHSSSTQNNDVAAANLVQWLRNSRRGLLVVGQIPPSQQDEVYPLLDQLAQTIGFPILA-TSSSLRFT---SHAVVWFAEHLLSCPNVREAVQPDFILQVGAPLLSTTITSYL----KQTAQHVILQTNARRRIDPEYSVTHSIST--PNMAYFLESLI----LRTSELPKGSISSALTPLV------------PWSRTLAP------------VMKELLLQQSP-------DRLTEPQIILALAQKSLP--QALFLSNSMPIRDAESFLY---PYGEIKRTVPLTVTGSNRGVSGIDGIIATAQGFALNAAEPTQLIIGDVSALHDLNSF-AFSSARK--------LSTLIVNNNGGAIFSFLPVAQHDVSFEEFFSTPTQ-HVDWEATARGLSVQY-KKVATYEALMNQWEQQSVNTLVEAVVISREENVQLHRDLTKKIQSHVSEHFWLDSTTRPKVLPLKRYTSNNANRTLLLLHGWMGCKEDWDDVVEE--------------------LLVHVGD---------DWNIVSIDLPGHN----HLVPSIESISSTTSGLNYSVSAMAEWIGDCLAQHYQLREVNAVAGYSLGGRIGLELSRLYSIPQVTTISSSLEPIPVDDSGRAQRDDKLSQQLQNIFQQQLVAANPTTMWGEFLESWYSAPIWGHLKERRPDTYRAILERRTAILQR-----------AGASIAQVLSEASPGRPENRRCEQDYFDRRHS---LLIGELDSKYS-------------LQSNATL---------ISESGHALLWESPKTIALHLAEFLSCN-GTSKNGDASRNNATVFVGNNRSSTAETLD-VTIATTSSGNEKSKESSILKDGQREGLLIVESLDYQVFSIQLLKDRNQGLGWGASAAASSTMQNRTGMIIELRAFN-YVGLGEVSPLKGVHDESLEQAQVQIQILQERLKLDS-PSISLIENIDALLDAWEQLLNE--TWYTSVRFGLEMALRSLLWHA-----NGMLPSS-------RGLLPLNGLQSR----------NSLIPQTQKWP---------------SLKVKVGHDSIERDQAAIYQ-GFRSTLG-KIRADANRAWDEEGALEFAFALEGWELQALNRLEYIEEPLQK---VNEAAWSLEQQIEALERFYLQASIPYALDESIGDLVQAHRRNWERIRENLRKSFSGGRSSRGCACFVLKPAVLGFALTTRIAKLAREELQIP----VVISSCMESGLGLAHAAFLAGEVDALPCNTPTLA----------HGLSTYSIL 1769          

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|299472374|emb|CBN77562.1| (Similar to Isochorismate Synthase [Ectocarpus siliculosus])

HSP 1 Score: 505.368 bits (1300), Expect = 7.765e-142
Identity = 332/764 (43.46%), Postives = 411/764 (53.80%), Query Frame = 0

Query:  336 PVSCEEPSQFDAWHEGVGKALAAMQAGEYRKVVLARAVHFTFPRKPRPLDLVLRLRGHYGYLFCLQLDADRAFLGCTPEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHK---------------DRFENRVTAESIEDALRPLVQGGRVERFPA--FVLRLQHVQHICHKITGRLR-GRSGAASESDTQEDWRE--SPETCSSSSAFDFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRSVLIVR-----------------------------ERAHVFAGAGIVPGSTAEGEWAEIGAKMANFVRMFP-RPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVHHDERGAGFYCLGYARARGRP----------------------------AAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQP-----DMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMA-----RGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYTCFPRLRPSCPLSSDLLAAIHD----AQRGLLVIGRLPQRRDRAHVRWLAQGLRWPIFADMGSGLRCDPALKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVASRAATL---IHVVPSP--ARRDPELSATHHVCSDIPTFVHNLDASL 1002
            PV+  E  ++  W  G+ +AL  M    Y KVVLAR     F     P +++ R RGHYG+LFCLQ DA  AFLGCTPE+LF   G  + TEALAGTR RG T  EDE L  ELM S K               D  EN +TA+ +  ALR   + G V+   +   + RL HVQHIC +IT  L  G     SE     D  E       +  +    L  +HPTPAV G PR      I ++E FDRGFYAGP+G++S  ++EF VAIRS L+                                   +FAGAGIVPGS A  EWAE G KM NF+ +FP RP   LR +PN+ +LW  LVVEEL R GV HF + PGSRS PL  A ARHP    + HHDERGAGFY +G+ARA                                 AV+ +SGTAVANLLPAA EA    + +LLLTADRPPE R CG+NQT+ Q      ++    AR + DV CPT EV A+ LLSD+D+AVS A      G  PGPVHLNFM RENLAP  GAVRD  VPGL S W D CL   R A+WE  ++P+T             + L  + D    A+RG+L++G L    +RA VR LA  LRWP++AD+ SGLRC  A   L V   + LL D  +  A+ PD +LQ+GSPL SKR+ KF ++  AATL   + V+ SP  A  DP    T  + S        +DA +
Sbjct:  524 PVTNTEGPEYRRWERGIERALDDMDKERYCKVVLARTARLRFAGPLSPPNVLARFRGHYGFLFCLQPDASTAFLGCTPERLFLSKGSTVVTEALAGTRGRGDTTTEDERLGEELMDSEKFRGGGGGLRVVLALVDVRENAITADYVSAALR---KAGAVKLNCSEPSLKRLTHVQHICRRITATLAPGTDNDCSEGGFCRDDAEGGGQGRGAGGAGRSLLREMHPTPAVCGTPRDVTYSVIGEVEGFDRGFYAGPVGYISAGASEFGVAIRSALVTDSWSSPEGFSRRGGVLGSAGGPAARGVGGGSEMTLFAGAGIVPGSVALLEWAETGVKMKNFLSLFPARPVRGLREMPNVNSLWGALVVEELVRLGVRHFCVCPGSRSAPLAVAIARHPSAVSISHHDERGAGFYAVGFARAGPGRGGGAHSSSTSDKAGGGGGGGVGSGALGMCAVVTSSGTAVANLLPAAAEADAAGLSMLLLTADRPPELRGCGSNQTIDQARCCMVNLLGSRARLSLDVQCPTDEVSAACLLSDIDHAVSRALGGGGGGGVPGPVHLNFMFRENLAPVEGAVRDAGVPGLSSAWSDRCLDSPRMARWEAGIEPFTTTAGGGGGLGRRGEGLQQVVDRLLCARRGILIVGSLIGPEERADVRHLAVTLRWPVYADVTSGLRCWSAEMGLGVGRLDQLLQDDAVKEAIAPDAVLQVGSPLTSKRVQKF-ISEPAATLTAPVSVICSPLVAWHDPGRGFTTRLASSTRDLAAAVDAGV 1283          

HSP 2 Score: 198.364 bits (503), Expect = 6.800e-47
Identity = 130/295 (44.07%), Postives = 153/295 (51.86%), Query Frame = 0

Query: 1079 LNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFAT--LASTAGAP-----SAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPI--ARHADVFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSS--------------LIEVVTDRAQNVRIHRQLGAQIRTTVHAGLSQLLRLDW-------IRTGP-------------------RTKPTLLLLHGFLGCKEDW 1324
            L EP  A  V+R +    GLFLS SMP RD+E FA   L    G       S    L+ VA NRGASGIDG+ SSAMGYAAGL    TLL+GDMA LHDL +LHAL G  A+ S     VT+V VNNGGGGIFSFLPI  ARH D FSP FD+PH + F  +  + GL Y    +A +  +A    Q                     IE VT R +N+ +HR LG   R      L + +RL W        RT P                   + KPTLLLLHGF+G KEDW
Sbjct: 1394 LTEPFVAWCVSRHIDPAAGLFLSASMPCRDVEVFADSGLGDLDGRGEEDRGSGGRRLSDVASNRGASGIDGVFSSAMGYAAGLRSAATLLIGDMATLHDLGSLHALKGLDAAGSC---SVTMVCVNNGGGGIFSFLPIADARHGDAFSPLFDTPHSVDFEGVCLSMGLEYRRATTAREFRSAYLESQGVLGGGGGEGAGTRRRRRHFFIEAVTCREENLPVHRSLGGIGRAAALDHLLRGVRLGWEFTPAPVARTWPPSPAGVSNGSLGRGRGGGDKKKPTLLLLHGFMGSKEDW 1685          

HSP 3 Score: 79.337 bits (194), Expect = 1.006e-10
Identity = 58/165 (35.15%), Postives = 86/165 (52.12%), Query Frame = 0

Query: 1910 IEYLEEPCKDPLCIPEVHKQTEQRLRFALDESLACPPLPYIELLSMEGLCALVLKPTVLGGLKQCLALHSLGLTKGLYSVVSSSFESSIGLAHLALLAAVLNSGTDTVRQIQTAGVHVLHGLSTYDVFVRSAEEWWGNGGGFSRLVASGSRSVLVDVVGCEELLD 2074
            +EY+EEP ++P  + +  +++   + +ALDESLA     + + L  EG  A V+K +V+GGL +   L  L    G  +V+SS+FE+ +GLAH ++LA+             T G  V HGLSTY               GF+  V+ G     VD + CEELLD
Sbjct: 2474 VEYIEEPLREPRSLGKFWERSGGIVPYALDESLAMGREKFTDKL--EGCAAFVVKVSVVGGLSRSARLCGLARRLGAEAVLSSAFETGVGLAHASILASCFT----------TPG--VAHGLSTYSRLQGDVAT-----PGFASTVSGGK----VDTLACEELLD 2615          

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|551657353|ref|XP_005831448.1| (hypothetical protein GUITHDRAFT_72208 [Guillardia theta CCMP2712] >gi|428175579|gb|EKX44468.1| hypothetical protein GUITHDRAFT_72208 [Guillardia theta CCMP2712])

HSP 1 Score: 434.106 bits (1115), Expect = 5.807e-131
Identity = 266/638 (41.69%), Postives = 363/638 (56.90%), Query Frame = 0

Query:  620 MANFVRMFPRPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPG--LHSEWPDACLGGRRFAQWERSLQPYTCFPRLRPSCPLSSDLLAAIHDAQRGLLVIGRLPQRRDRAHVRWLAQGLRWPIFADMGSGLRCDPA-LKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVA---SRAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTAGAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDT--PVTVVLVNNGGGGIFSFLPIARHADVFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLV 1249
            M N +++F  P  PL SLPN+  LWA L VEEL R GVT F + PGSRSTPL  A + +   T +V HDER AGFYC+GYARA+ +PAA IVTSGTAVANLLPAAVEA+   +P+LLLTADRP E RD G+NQT+ Q  +F  + RW++D+ CP+ ++ A+ LLSDVD+AV  A  +  GPVHLNF  RENLAP+GG VRD   PG  + S W  AC+     A+WE S  P+T +P       + +++L+++  A+RGL+V+G L    D A V W+A  L WP++ D  SG R D        V+  + +L +  +S AL PD ILQ G  LVSKR+   + +   S   + + V  +P R+DP    TH++      F   L   +   R        S LL L             E++   A T              AL  SL           +  + L EP+ A   +  L  G  LF+S+SMP+RD++ F   A +   P        +  NRGASGIDGI+SSA+GYA G G+P+TLL+GD A L+DL+A+  +         PD   PV +V+VNNGG GIF FLPIA+H ++   +F + H + F  + ++FG+ Y    +AG L+
Sbjct:    1 MRNILQLFANPPRPLSSLPNINMLWAVLTVEELCRCGVTVFCLCPGSRSTPLAVAVSWNAKATSVVMHDERSAGFYCVGYARAKRKPAACIVTSGTAVANLLPAAVEASQQGLPMLLLTADRPFELRDNGSNQTIEQAGIFGRYVRWSRDLCCPSDQIPANVLLSDVDFAVHRASSSPRGPVHLNFQFRENLAPDGGPVRDNP-PGNPISSTWSRACIASLNMAKWEHSALPHTLYPSSSSHVEVPAEVLSSMARARRGLVVVGNLFTAEDMATVGWVAAELCWPVWCDAQSGGRWDFGPFGMRGVSMMDQMLTNQRVSGALRPDFILQFGERLVSKRILSLISSCCYSDRCSYVVVSDAPGRQDPSAIVTHYLSCSPTDFGKQLSRWMQHNR-----IYPSDLLPL-------------EKMSEHART--------------ALWSSL-----------SGYKDLEEPLIALRCSESLARGRALFVSSSMPIRDVDMFFHAALSRERP-------EIGCNRGASGIDGIVSSALGYAGGRGRPLTLLLGDTAALYDLSAIRHV---------PDCPHPVVLVIVNNGGCGIFRFLPIAQHEEMMEKFFINSHQIDFESLARSFGIDYMHAATAGWLL 578          

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|1026755720|gb|OAE18270.1| (hypothetical protein AXG93_723s1220 [Marchantia polymorpha subsp. ruderalis])

HSP 1 Score: 464.922 bits (1195), Expect = 1.680e-129
Identity = 381/1174 (32.45%), Postives = 534/1174 (45.49%), Query Frame = 0

Query:  242 YGGGRFDPQYPHPSAEWQGFGASYLVLPALELIRRTRHGDGPLDGSFLLALNLRFSNVQELQAGRTALLHLLRDVQVAATD--GDDEGAGDALLPAPVSCEEPSQFDAWHEGVGKALAAM-QAGE--------------YRKVVLARAVHFTFPRKPRPLDLV--LRLRGHYGYLFCLQLDADRAFLGCT-----------------------------PEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHKDRFENRVTAESIEDALRPLVQGGRVERFPAFVLRLQHVQHICHKITGRLRGRSGAASESDTQEDWRESPETCSSSSAFDFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRSVLI-------VRERAHV---------------------------------------------FAGAGIVPGSTAEGEWAEIGAKMANFVRMFPRPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYT----CFPRL----RPSCPLSSDLLAA-IHDAQRGLLVIGRLPQRRDRAHVRWLAQGLRWPIFADMGSGLRCDPAL--------KHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVASRAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLA--------STAGAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHA--DVFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIEVVTDRAQNVRIHRQLGAQIRTTV 1288
            YG  RF+P+   PS EWQ FG+ Y  +P +EL           +G  LLA+N+ + N       + AL + L  +Q  +T    D+E     +L      +EPS    WH+ V K L  +   GE                KVV+AR        +  P+ L+  L+ +    Y F +Q+    AF+G +                             PE+LF   G  +++EA+AGTR RG    +D  L  ELM S KD  E  +  ES++  ++ + +    E     +++ + VQH+  +  G L                         ++ FD L+ LHPTPAV G P+  A  AI   E FDRG YAGP+G++    AEFAV IRS LI         E+ H+                                             +AG GIV  S +  EW E+  K + F  +   P  PL  + N+  LWA L+VEE  R G+T+F IAPGSRS+P+  AAA +P  T +   DER  GF+ +GY R   + AA+I +SGTAV+NLLPA VE++ D VPLL++TADRP E  D GANQT+ Q   F  F R+  ++P    ++ A  +L+ VD AV+ A  A  GPVH+N   RE LA            G    W  ACL G    +W  +  PYT    C P+L     P+     + +A  +  A  G++++G L    +   V  LAQ L WP+  D+ SGLR    +        K  ++  ++ +L    ++ +L PDV+LQIGS L SKRL +F+ A+     I V    +R DP    TH V S    F + +  +       KP   ++   S L+ L+  ++ E+   L  E+                                      L EP  AH V   LP+   LFL NSMP+RD + +A  A          +   S P   A V  NRGASGIDG+LS+A+G+AAG  + VTLLVGD++ LHD N L  L     S      PVTVV+VNN GGGIFS LP+A       F+  F +PH +    + QA  + +   KS  DL  AL   Q    + +IEV +   +N   HR L   I   V
Sbjct:  247 YGAMRFNPE-TEPSKEWQPFGSFYFFIPQIELCE--------CEGCSLLAVNVAWDNSMNWTIMK-ALNNSLIVMQTVSTRICPDNERPLAQVLRREHVPDEPS----WHDAVEKTLQLLCDRGEEISDNGAVSSVHPSISKVVMARRTKLELDDQLDPVSLLACLQEKDPSAYQFAIQVPDGSAFIGSSGKAVAHRSQMSWGKRTIQANEQAKIFLGQPERLFARDGLLVASEAVAGTRARGSGALKDFQLGMELMLSTKDHHEFDIVRESVKRHMKMVCKDVETE-VNKCLIKQERVQHLYARFNGEL----------------------ADEAAEFDLLSVLHPTPAVCGHPQAAAKDAISTSESFDRGLYAGPVGWVGGGGAEFAVGIRSSLIQPGTSDKSPEQPHIPLTNDAASKLSISKDSVNNRIPASSSQRSPASKKPLEGKGTCIFLYAGVGIVKDSKSSSEWRELELKTSQFEALL-EPRQPLEKVVNMNALWAKLIVEECCRLGITYFCIAPGSRSSPIAAAAAANPRITCVPCIDERSMGFHAVGYGRGANKAAALITSSGTAVSNLLPAVVESSQDFVPLLIITADRPAELHDSGANQTIDQGKHFAGFVRYHANLPAADDKMPARMVLTTVDSAVNKAMSAPSGPVHINIGFREPLA------------GTPQAWNAACLKG--LERWIDTAAPYTQYIDCNPKLLGGVHPTYVADLEGIAELLESATEGMIIVGGLSAAEETWAVALLAQHLGWPVVPDILSGLRIGKVMCSSRVGERKVNIIHNFDQVLLSSSVAQSLRPDVVLQIGSRLTSKRLSQFLEAASPEAYIIVEEHSSRHDPSHIVTHRVQSSAADFSYTIMNATG----SKP---RTSFCSRLQILSEVVQREVFLRLQAESS-------------------------------------LTEPYVAHYVVSCLPSDGALFLGNSMPIRDSDMYAHGALNLIGDKHEDSWEQSLPHLGARVGANRGASGIDGVLSTAIGFAAGSRQRVTLLVGDVSFLHDTNGLTLL-----SERVGQPPVTVVVVNNRGGGIFSLLPVAETMPEASFTTLFTTPHTVELKKLCQAHSVDHWYVKSKEDLKTALQVVQRSPLNCVIEVESTIEENADFHRFLQVSIGQAV 1319          

HSP 2 Score: 120.939 bits (302), Expect = 2.098e-23
Identity = 82/278 (29.50%), Postives = 126/278 (45.32%), Query Frame = 0

Query: 1305 PRTKPTLLLLHGFLGCKEDWTPLLWEAGGCASASNAGLSCSSSSPNTIVGVSGGSGGRGLAMDYDCLAVELPGHGTTAMHGAGVG--------------CSAAYSMPVVAFSLIQLLDRLGIAQCAVMGYSLGGRLAMYLAQQYPQRISAMIIVSAHPGLRAEGERLDRREEERRLGRRIEEAAHEGGEAWSAFLRWWYSKPLWGRLAERSPSYARMMRRRRAALARSEDPGGLSRAFAGMSLAQQPALWDFLRSGHVPTLIVYGANDVKYAGLGEEL 1568
            P  KPTLL LHGFLG  EDW P++                                   L++ + C++V+LPGHG TA+  +                    A+S+  +A +L +L  R+G     ++GYS+G R+A+++A    Q ISA ++VS  PGL +   R  R   +  L   +     EGG    +F+  WYS+P W  L    P +  + R R      ++D   L+++ +  S  +Q +LW  L  G VP L V G  D K+ GL  ++
Sbjct: 1792 PDKKPTLLFLHGFLGNGEDWLPVM---------------------------------DALSLTFRCISVDLPGHGHTAVETSMSSPLQSRSKAESDFSDADGAWSVQGLASALSELAQRVGEHNVILVGYSMGARIALHMALNNNQGISAAVVVSGSPGLHSSAARDIRAAHDDALAASL----CEGG--IHSFVDTWYSQPTWKSLRAH-PDFELIKRSR----VENQDVHSLAKSLSSFSTGRQRSLWGDLSMGEVPLLFVVGQEDTKFVGLAHQM 2025          

HSP 3 Score: 99.3673 bits (246), Expect = 7.811e-17
Identity = 116/395 (29.37%), Postives = 181/395 (45.82%), Query Frame = 0

Query: 1668 RVASLQWTTCSVALKKP----LVLSSGPPLTHRRVIILVLEAVSGSVGVGEASPLPGFSKESFLEAEKQVKKISVALRGRTLPKLGGSKGLAAWKNWLE-----SDVSLLPSVRAGLEMALVHLLAQEYRVDIPGVLKAGYPTHLLHTGQVCLNGLVLRTSPSLPSVPPSSLMSPTPSMPSSASFTASMDTSTSSSSKIKVGGR--PVKE-DVARVNNLTAELTFGHR--LRLDANQAWSADEAVSFIRGLSHPEKIEYLEEPCKDPLCIPEVHKQTEQRLRFALDESLACPPLPYIELLSME---GLCALVLKPTVLGGLKQCLALHSLGLTKGLYSVVSSSFESSIGLAHLALLAAVLN---------SGTDTVRQIQTAGVH--VLHGLSTY 2034
            R++  ++     ALK P    +  SS    T R   ++ +    G+ G+GE  PL G  +ES L+AE+Q++ +   L+G  L          ++  W        + ++LPSVR GLEMAL+  L       +  +L  G P H   TG V +N           +V    L+    S    A   A +      + K+KVG R  P+++ DV +    T     G R  LR DAN+ WS  +A+ F   +     +++LEEP ++   + +  KQT   L  ALDE+L    L    LL M    G+ A+V+KP+ +GG +    +      +G+  V+SS+FESS+ LA LA  AA ++         +  D+      A +   + HGL TY
Sbjct: 1342 RISDAEYIQHRFALKAPPTTKMKRSSSGDET-REGFLVRVTLFDGTSGIGEIVPLEGLHEESLLDAEEQLRVVITKLKGLHLLSTLALLN-GSFGEWFTGVAGIQEKNILPSVRMGLEMALLAALGSTRSCSLLQLL-CGVP-HSTLTGPVDINS----------NVRVCGLLDSEGSAEDVAEAAADLVKEGFCTLKLKVGRRSSPLEDADVLK----TIRKRVGPRICLRADANRKWSLQQALLFAESVPSC-GLQFLEEPVRNLQDLVQFCKQT--LLPVALDETLDENLLNVETLLPMFARIGVVAVVIKPSRVGGFEHAYRIAQYAQLQGMMVVISSAFESSMTLATLAQFAAYVDWKRLRAMPSATYDSSENQPNASIFSPIAHGLGTY 1715          

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|1199290403|gb|OUS45503.1| (hypothetical protein BE221DRAFT_193196 [Ostreococcus tauri])

HSP 1 Score: 462.611 bits (1189), Expect = 5.786e-129
Identity = 347/942 (36.84%), Postives = 475/942 (50.42%), Query Frame = 0

Query:  365 RKVVLARAVHFTFPRKPRPLDLVLRL--RGHYGYLFCLQLDADRAFLGCTPEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHKDRFENRVTAESIEDALRPLVQGGR----VERFPAFVLRLQHVQHICHKITGRLRGRSGAASESDTQEDWRESPETCSSSSAFDFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRSVLI--VRERAHVFAGAGIVPGSTAEGEWAEIGAKMANFVRMFPRPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYTCFPRLRPSCPLS----SDLLAAIHDAQRGLLVIGRLPQRRDRAHVRWLAQGLRWPIFADMGSGLR---CDPALKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVAS---RAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTA------GAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHAD--VFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIEVVTDRAQNVRIHRQL 1280
            RKVVLAR             DL+ RL  R    Y F L+      FLG TPE+LF   GG  S+EA+AGTR RG    ED ALA +++ S K+  E  +  E +  AL  + +GG     VE     +LR   VQH+  +++  L G    A                      D + ALHPTPAV G PR  AL+ +R +E FDRG Y+GP+G++  E AEFAVAIRS L+    +   ++AG G+V  + A+ EW+E+  K      +   P+P L SLPN    WAT++V EL R G+T F +APGSRSTPL  AA +H    ++V  DER  GFY LGYA+  G+PAAVI +SGTAVANLLP+ VEA+    PLLLLTADRP E R+ GANQT+ Q  +F  + R+  D+  P     A +  +    A+   RG D GPVHLN   RE L P+              EW D+    +    WE S   +T       S P         L+ I  A+RGL V+G      D      +A+ L W + AD  SG+R   C+ A    ++   + +L +P     L PDVI+Q+G+ L SKRL +F+  S     A  I + P+P R DP    +  V S I      L+ +L+ E       R++  ++                    AD     G      +  AL+   +               ++E   A  ++  LP   GLFL NSMP+RD++TFA +   A         S   P   V+ NRGASGIDG++S+A GYAAGLG PVTL++GD++  HD N L  L            PVTVV+VNNGGGGIFSFLP+A   D   F+  F +P D+    + +A  + Y   ++  +L  AL +     + ++IEV T RA+N+  H+ +
Sbjct:  443 RKVVLARRSKLALSAPVNTFDLISRLQARDPDAYQFVLRHPNGETFLGSTPERLFLSQGGRASSEAVAGTRARGSDDGEDAALAYDMLLSPKEHEEFAIVREEVRRALGEVAEGGNDGVDVE-LEKGILRNVTVQHLYSRLSAPLAGGKNEA----------------------DLICALHPTPAVCGYPRPAALRTLRAVESFDRGLYSGPLGWIGAEDAEFAVAIRSALVHPSGDEVSLYAGVGVVGSANAQAEWSELNLKTKPLESLLA-PAPRLASLPNPNAAWATILVGELVRGGITTFCVAPGSRSTPLALAAEKHVGANVVVCIDERSLGFYALGYAKGSGQPAAVICSSGTAVANLLPSVVEASESSTPLLLLTADRPYELRNTGANQTIDQVKIFGSYTRFNADLAPPGDGSPARACATMASTALRHLRGVDSGPVHLNCAFREPLGPQ------------RVEW-DSVTALKGLENWEASSTQFT-IGNASISAPQDGSAWQSALSRISRAKRGLFVVGGGTTAADAIAATAMAETLGWAVAADATSGMRVGACENASVR-MIPMIDYVLVEPSTHEVLRPDVIVQVGTRLTSKRLNQFLETSAIDHGAEWIIIEPTPNRHDPAHCVSVRVESSIGHASELLERALNDESGYAMSERKAACVAF-------------------ADRAVSIGEAVAREAAVALDDITINEG------------VSEMAVAVAISEGLPDSMGLFLGNSMPIRDIDTFAGIKRYAEGFEKSDGTSLGVP---VSANRGASGIDGVISTAAGYAAGLGHPVTLIIGDVSFQHDSNGLLFLRERPGQ-----PPVTVVVVNNGGGGIFSFLPVAAQVDDSSFNRLFATPPDVSRRGLCEAHRVTYSHPRTIEELRRALRTSWTENQHNVIEVTTSRARNLVQHKMV 1306          

HSP 2 Score: 98.9821 bits (245), Expect = 9.757e-17
Identity = 104/370 (28.11%), Postives = 160/370 (43.24%), Query Frame = 0

Query: 1669 VASLQWTTCSVALKKPLVLSSGPPLTHRRVIILVLEAVSGSVGVGEASPLPGFSKESFLEAEKQVKKISVALRGRTLPK----LGGSKGLAAWKNWLESDV-------SLLPSVRAGLEMALVHLLAQEYRVDIPGVLKAGYPTHLLHTGQVCLNGLVLRTSPSLPSVPPSSLMSPTPSMPSSASFTASMDTSTSSSSKIKV----GGRPVKEDVARVNNLTAELTFGHRLRLDANQAWSADEAVSF-IRGLSHPEKIEYLEEPCKDPLCIPEVHKQTEQRLRFALDESL------------ACPPLPYIELLSMEGLCALVLKPTVLGGLKQCLALHSLGLTKGLYSVVSSSFESSIGLAHLALLAAVL 2010
            V  ++  + S+ L KP    S      R  +IL +    GSVG GE SPLPG  +E+  E+  Q+  ++  L    +P     L G     A+ +WLES V        L PSVR           A E  V        G P   + TG    +G+ +           + L+     + ++      +  +  +  K+KV    G      D  R+  + AE+     LR DAN+ W  ++A+ F  R +     ++Y+EEP +D   I   H  T   +  ALDE++            A   +  I   S  G+ A+VLKP+V+GGL+    L    +++G+  V+SS+FES+IGL   A LA  L
Sbjct: 1329 VRGVEIASFSIPLNKPTTTVSAS--MQREGMILKVTLSDGSVGFGECSPLPGLHRETLQESRAQLAVVASLLDNIDVPTNISLLNG-----AFTSWLESRVGIRTPNGELFPSVR----------FAVEAAVANALAASVGEPLAHIMTGNADTSGVFV-----------NGLVDAATDIDAARREARDLVDAGFTCLKVKVARGVGKEGANADAERLAAIRAEIGPSVVLRADANKGWDLNDALHFGARVVDLGVNLQYVEEPTRDARDIATFHFTTS--IPVALDETIDEIVRSSANFADASATVAQIADQST-GVAAIVLKPSVVGGLEATTTLARAAMSRGVRPVISSAFESNIGLNTCAHLAGFL 1667          

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|693496457|emb|CEG00640.1| (Mandelate racemase/muconate lactonizing enzyme,C-terminal [Ostreococcus tauri])

HSP 1 Score: 462.611 bits (1189), Expect = 6.564e-129
Identity = 347/942 (36.84%), Postives = 475/942 (50.42%), Query Frame = 0

Query:  365 RKVVLARAVHFTFPRKPRPLDLVLRL--RGHYGYLFCLQLDADRAFLGCTPEQLFRVAGGAISTEALAGTRPRGQTPEEDEALAAELMASHKDRFENRVTAESIEDALRPLVQGGR----VERFPAFVLRLQHVQHICHKITGRLRGRSGAASESDTQEDWRESPETCSSSSAFDFLAALHPTPAVAGEPRLTALQAIRQLEPFDRGFYAGPIGFLSRESAEFAVAIRSVLI--VRERAHVFAGAGIVPGSTAEGEWAEIGAKMANFVRMFPRPSPPLRSLPNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYTCFPRLRPSCPLS----SDLLAAIHDAQRGLLVIGRLPQRRDRAHVRWLAQGLRWPIFADMGSGLR---CDPALKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVAS---RAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTA------GAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHAD--VFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIEVVTDRAQNVRIHRQL 1280
            RKVVLAR             DL+ RL  R    Y F L+      FLG TPE+LF   GG  S+EA+AGTR RG    ED ALA +++ S K+  E  +  E +  AL  + +GG     VE     +LR   VQH+  +++  L G    A                      D + ALHPTPAV G PR  AL+ +R +E FDRG Y+GP+G++  E AEFAVAIRS L+    +   ++AG G+V  + A+ EW+E+  K      +   P+P L SLPN    WAT++V EL R G+T F +APGSRSTPL  AA +H    ++V  DER  GFY LGYA+  G+PAAVI +SGTAVANLLP+ VEA+    PLLLLTADRP E R+ GANQT+ Q  +F  + R+  D+  P     A +  +    A+   RG D GPVHLN   RE L P+              EW D+    +    WE S   +T       S P         L+ I  A+RGL V+G      D      +A+ L W + AD  SG+R   C+ A    ++   + +L +P     L PDVI+Q+G+ L SKRL +F+  S     A  I + P+P R DP    +  V S I      L+ +L+ E       R++  ++                    AD     G      +  AL+   +               ++E   A  ++  LP   GLFL NSMP+RD++TFA +   A         S   P   V+ NRGASGIDG++S+A GYAAGLG PVTL++GD++  HD N L  L            PVTVV+VNNGGGGIFSFLP+A   D   F+  F +P D+    + +A  + Y   ++  +L  AL +     + ++IEV T RA+N+  H+ +
Sbjct:  443 RKVVLARRSKLALSAPVNTFDLISRLQARDPDAYQFVLRHPNGETFLGSTPERLFLSQGGRASSEAVAGTRARGSDDGEDAALAYDMLLSPKEHEEFAIVREEVRRALGEVAEGGNDGIDVE-LEKGILRNVTVQHLYSRLSAPLAGGKNEA----------------------DLICALHPTPAVCGYPRPAALRTLRAVESFDRGLYSGPLGWIGAEDAEFAVAIRSALVHPSGDEVSLYAGVGVVGSANAQAEWSELNLKTKPLESLLA-PAPRLASLPNPNAAWATILVGELVRGGITTFCVAPGSRSTPLALAAEKHVGANVVVCIDERSLGFYALGYAKGSGQPAAVICSSGTAVANLLPSVVEASESSTPLLLLTADRPYELRNTGANQTIDQVKIFGSYTRFNADLAPPGDGSPARACATMASTALRHLRGVDSGPVHLNCAFREPLGPQ------------RVEW-DSVTALKGLENWEASSTQFT-IGNASISAPQDGSAWQSALSRISRAKRGLFVVGGGTTAADAIAATAMAETLGWAVAADATSGMRVGACENASVR-MIPMIDYVLVEPSTHEVLRPDVIVQVGTRLTSKRLNQFLETSAIDHGAEWIIIEPTPNRHDPAHCVSVRVESSIGHASELLERALNDESGYAMSERKAACVAF-------------------ADRAVSIGEAVAREAAVALDDITINEG------------VSEMAVAVAISEGLPDSMGLFLGNSMPIRDIDTFAGIKRYAEGFEKSDGTSLGVP---VSANRGASGIDGVISTAAGYAAGLGHPVTLIIGDVSFQHDSNGLLFLRERPGQP-----PVTVVVVNNGGGGIFSFLPVAAQVDDSSFNRLFATPPDVSRRGLCEAHRVTYSHPRTIEELRRALRTSWTENQHNVIEVTTSRARNLVQHKMV 1306          

HSP 2 Score: 98.9821 bits (245), Expect = 1.001e-16
Identity = 104/370 (28.11%), Postives = 160/370 (43.24%), Query Frame = 0

Query: 1669 VASLQWTTCSVALKKPLVLSSGPPLTHRRVIILVLEAVSGSVGVGEASPLPGFSKESFLEAEKQVKKISVALRGRTLPK----LGGSKGLAAWKNWLESDV-------SLLPSVRAGLEMALVHLLAQEYRVDIPGVLKAGYPTHLLHTGQVCLNGLVLRTSPSLPSVPPSSLMSPTPSMPSSASFTASMDTSTSSSSKIKV----GGRPVKEDVARVNNLTAELTFGHRLRLDANQAWSADEAVSF-IRGLSHPEKIEYLEEPCKDPLCIPEVHKQTEQRLRFALDESL------------ACPPLPYIELLSMEGLCALVLKPTVLGGLKQCLALHSLGLTKGLYSVVSSSFESSIGLAHLALLAAVL 2010
            V  ++  + S+ L KP    S      R  +IL +    GSVG GE SPLPG  +E+  E+  Q+  ++  L    +P     L G     A+ +WLES V        L PSVR           A E  V        G P   + TG    +G+ +           + L+     + ++      +  +  +  K+KV    G      D  R+  + AE+     LR DAN+ W  ++A+ F  R +     ++Y+EEP +D   I   H  T   +  ALDE++            A   +  I   S  G+ A+VLKP+V+GGL+    L    +++G+  V+SS+FES+IGL   A LA  L
Sbjct: 1329 VRGVEIASFSIPLNKPTTTVSAS--MQREGMILKVTLSDGSVGFGECSPLPGLHRETLQESRAQLAVVASLLDNIDVPTNISLLNG-----AFTSWLESRVGIRTPNGELFPSVR----------FAVEAAVANALAASVGEPLAHIMTGNADTSGVFV-----------NGLVDAATDIDAARREARDLVDAGFTCLKVKVARGVGKEGANADAERLAAIRAEIGPSVVLRADANKGWDLNDALHFGARVVDLGVNLQYVEEPTRDARDIATFHFTTS--IPVALDETIDEIVRSSANFTDASATVAQIADQST-GVAAIVLKPSVVGGLEATTTLARAAMSRGVRPVISSAFESNIGLNTCAHLAGFL 1667          

BLAST of EWM26091.1 vs. NCBI_GenBank
Match: gi|1085573954|gb|OGX27898.1| (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase [Omnitrophica WOR_2 bacterium RIFCSPHIGHO2_02_FULL_48_11])

HSP 1 Score: 410.609 bits (1054), Expect = 1.677e-122
Identity = 243/654 (37.16%), Postives = 351/654 (53.67%), Query Frame = 0

Query:  638 PNLATLWATLVVEELARNGVTHFVIAPGSRSTPLVTAAARHPDTTLMVHHDERGAGFYCLGYARARGRPAAVIVTSGTAVANLLPAAVEAATDRVPLLLLTADRPPEQRDCGANQTVAQPDMFLHFARWTKDVPCPTPEVLASSLLSDVDYAVSMARGADPGPVHLNFMLRENLAPEGGAVRDTAVPGLHSEWPDACLGGRRFAQWERSLQPYTCFPRLRPSCPLSSDLLAA--IHDAQRGLLVIGRLPQRRDRAHVRWLAQGLRWPIFADMGSGLRCDPALKHLLVAPYESLLADPLISSALDPDVILQIGSPLVSKRLPKFMVASRAATLIHVVPSPARRDPELSATHHVCSDIPTFVHNLDASLDWERRRKPEARQSGLLSLLKTLAARIEHEIAEELGPEADTLGPAGPGPFPSSPSALNPSLVPSSSPPSPAPTDSRLLNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTAGAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGGGGIFSFLPIARHADVFSPYFDSPHDLHFGPITQAFGLPYHLCKSAGDLVAALHSCQAGGRSSLIEVVTDRAQNVRIHRQLGAQIRTTVH 1289
            PNL  LWA L+VEEL RNGV +F +APGSRS+PLV A A        +H DERG  F+ LGY  A  +P A+I TSGTAVAN  PA +E +  ++PL++LTADRPPE R  GA+QT+ Q ++F  + RW  DVPCPT ++    +L+ VD AV  A+ +  GPVHLN M RE LAP               E     L G   A W++  +P+T + + + + P  +++  A  +   Q+G++V+G+L    +   V  LAQ L WP+F D+ SGLR       ++    + LL+D  +  A+  D +L +G  + SKR  +F+  SR    I V+  P R DP  + T  V S +  F   L  S+         A+++ L+ +L+  +  +  +I + L                                     + S+ L E   A  ++ L+PAGHGLFL++SMP+RDM+ +A+            P+  V  NRGASGIDGI++SA G++ GL KPVTL++GD+A LHDLN+L  L            PVTVV++NN G GIFSFLPIA+  ++F  YF +PH L F    Q F L Y   +S  + V    +  +   S++IE+ TDRA N + H+ L   +   ++
Sbjct:    7 PNLNQLWADLLVEELVRNGVEYFCLAPGSRSSPLVAAVAAQKKAKSFIHFDERGVAFHALGYVAATRKPCALICTSGTAVANFFPAIIETSKKKLPLIVLTADRPPELRLSGADQTIDQVNIFGDYVRWFADVPCPTLKIKPEFVLTTVDQAVYRAQSSVSGPVHLNCMYREPLAPLAT-----------KEKFSNYLSG--LAHWQKGDRPFTLYKKSKHNVPFETNIFVADTLSRIQKGVMVVGKLANEAEAEAVLKLAQKLNWPVFPDITSGLRLGKRHSQIISYFDQILLSDKGV-KAMSIDGVLHLGGRITSKRWYQFIEKSRPQHYIMVLNHPLRSDPLHNVTLRVESSVQNFCEGLQQSI-------LPAQENRLIKILQKASEIVGQDIRKFL-------------------------------------SKSKKLTEINVARSISELVPAGHGLFLASSMPIRDMDMYASPGKN--------PVV-VGANRGASGIDGIIASAAGFSVGLNKPVTLVLGDLAFLHDLNSLAMLQNCP-------QPVTVVVINNNGSGIFSFLPIAQFKNIFEQYFGTPHGLTFSQAAQLFNLKYQHPRSPEEFVKDYKAALSANSSTIIEIRTDRAANYKSHKDLQQNLTLKIN 586