EWM26188.1, cds4477 (CDS) Nannochloropsis gaditana

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|585108185|gb|EWM26188.1| (Pheophorbide a oxygenase [Nannochloropsis gaditana])

HSP 1 Score: 1141.33 bits (2951), Expect = 0.000e+0
Identity = 555/555 (100.00%), Postives = 555/555 (100.00%), Query Frame = 0

Query:    1 MACFMFLRLLVVVGTIASPITAFFTSSGSPLFRCVLPAKTSPPQPPHAVGPFATVAQPAPTPVSPMPPSSTTSSSSLTNAPSSSGSLQPLGPGHGSGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWADHDKTWHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAEPEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHDPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEGVFLSLPRLANSTIEFHAPTRLHYLFDFTGFFQRVPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAINKPRWLDHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAFYMPTKSDEGVRAFRQWLVRWGAGGPRWADGVDSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSLVCMTAIWTPRPASVLGKILGAGVAMAFGSWQMARLEKRMLFIDYVHAHK 555
            MACFMFLRLLVVVGTIASPITAFFTSSGSPLFRCVLPAKTSPPQPPHAVGPFATVAQPAPTPVSPMPPSSTTSSSSLTNAPSSSGSLQPLGPGHGSGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWADHDKTWHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAEPEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHDPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEGVFLSLPRLANSTIEFHAPTRLHYLFDFTGFFQRVPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAINKPRWLDHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAFYMPTKSDEGVRAFRQWLVRWGAGGPRWADGVDSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSLVCMTAIWTPRPASVLGKILGAGVAMAFGSWQMARLEKRMLFIDYVHAHK
Sbjct:    1 MACFMFLRLLVVVGTIASPITAFFTSSGSPLFRCVLPAKTSPPQPPHAVGPFATVAQPAPTPVSPMPPSSTTSSSSLTNAPSSSGSLQPLGPGHGSGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWADHDKTWHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAEPEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHDPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEGVFLSLPRLANSTIEFHAPTRLHYLFDFTGFFQRVPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAINKPRWLDHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAFYMPTKSDEGVRAFRQWLVRWGAGGPRWADGVDSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSLVCMTAIWTPRPASVLGKILGAGVAMAFGSWQMARLEKRMLFIDYVHAHK 555          

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|873223994|emb|CEM27529.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 322.783 bits (826), Expect = 1.121e-100
Identity = 193/506 (38.14%), Postives = 272/506 (53.75%), Query Frame = 0

Query:   93 GHGSGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWAD-HDKTWHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAEPEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHD--PAFI--RGAWVTRDLM-YGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEGVFL------------SLPRLANSTIEFHAPTRLHYLFDFTGFFQR-----VPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAINKPRWLDHLERNAVLDGDLVFLHGQEREMR-RPELKWEENIDRAFYMPTKSDEGVRAFRQWLVRWGAGGPRWADGVDSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSLVCMTAIWTPRPASVLGKILGA--------GVAMAFGSW-------------QMARLEKRMLFIDYVHA 553
            G  +G   DW RQWYP++    LD   P +V LL + FV+W D   + W A +DQCPHRLAPLSEGR  E G+LQC YHGW F G G C  IPQ E   K  A  + RA  + VP  + QG++W++PD++ +G EAS+S  P+TVPEL D  P  +  R   + R ++ Y +DTL+EN+VDP+HVP+AHHG+Q  R  + P+ +      + G F               P   ++TI F  P  + Y F+  G  +      +  +  +  L   +  L  D R    L+ Y IP+ PG+S +L+ FPR+    ++P W DHLER AVLDGD + LH QER MR +P+       ++++YMP ++D  VR FR WL  +G G P W  G    LPP   DR  ++DR+  HT  C  CR A    + L+ V+ A++ VC+       P + LG +L          G A++   W              +A +E+R +FIDYVHA
Sbjct:    2 GEAAGPSFDWGRQWYPVSVAKHLDKRLPQKVTLLSEHFVLWWDKQHQAWKAAKDQCPHRLAPLSEGRFTEEGHLQCTYHGWHFDGDGKCRGIPQIEEVSKCSALDNPRAHLEVVPCQLKQGILWIFPDSTAEGREASQSVQPATVPELDDGPPGLVASRDTGLKRTIVHYNWDTLIENLVDPSHVPWAHHGIQGDRKKSRPIPLQRASKLETGGFTVSSKATLGQLRSGDPVEIDNTIRFKPPCLVMYAFNLAGVGKMLREKFIVRLLCSLGLGPRVAALDTDGRNMFYLILYAIPIEPGKSMVLSRFPRSTGKTSRPEWHDHLERMAVLDGDSIHLHWQERMMRLKPD--GVRLFEQSYYMPAQADLAVRLFRNWLGNYGGGQPSWLQG-SGELPPLQRDRRMILDRWLNHTKDCPYCRTAFCVFKSLRIVSTALA-VCL-------PFTALGVMLTQTLLAPKERGRAVSLSGWLCAVASVGGVAAPLLASVEQRYVFIDYVHA 496          

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|873230244|emb|CEM11347.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 322.013 bits (824), Expect = 2.869e-99
Identity = 203/557 (36.45%), Postives = 280/557 (50.27%), Query Frame = 0

Query:   51 PFATVAQPAPTPVSPMPPSSTTSSSSLTNAPSSSGSLQPLGPGHGSGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWADH-DKTWHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAEPEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHD-PAFIR---GAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEG-VFLSLPRLANST----IEFHAPTRLHYLFDFT---GFFQRVPLMKWAFRLEKWLRRLPPDTRPRSS---------LVTYTIPVGPGRSRILALFP-------RNFFAINKPRWLDHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAFYMPTKSDEGVRAFRQWLVRWGAGGPRWADGVDSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSL---------VCMTAIWTPRPASVLGKILGA---GVAM-----------AFGSWQMARLEKRMLFIDYVHAHK 555
            P A +  P+P     MPP +     S T A S +   +  G         DWWRQWYP+A   DL   +P Q  +L + FV+W D   + + A +D CPHRLAPLSEGRI + G L C YHGW F G G C  IPQ E + +  A ++ RAC + VP   A  ++W++PD++ +G  ASR   P+TV E    P  I+        R L Y YDTLVENV DP+HVPFAHHG+Q  R+ A P+ + ++ +   G    S+  L  +     I F  P  L+Y  DF+   G  ++ P++    RL   +   P  T+P  S         LV Y +P  PG+S I+  F        RN  ++   RW DHLE  AV DGD + +H QER +R  E    E  DRA+YMP  +D  VR+FR+WL+  G G P W  G    L P   DR  L DR+  HT  C  CR A    RVL+ +T  +S+         V   A   P P  +L + L +   G A+            +G++++  LE+R ++ DY H  +
Sbjct:   50 PSAVLTAPSPA-AKTMPPRTDNIELSFTPAASEASEKEDAGT-------FDWWRQWYPVAIASDLGKKQPRQFTVLNEHFVVWWDEATQKFAAAKDACPHRLAPLSEGRISDDGKLHCTYHGWEFDGTGACTKIPQVEAKAQEAARKNKRACLQTVPCTEAHALIWIFPDSTREGWAASREVEPATVAEFDTVPELIKTKDNGLFFRVLNYNYDTLVENVGDPSHVPFAHHGIQGNRNRAGPIRMKVDNLTDAGFAVTSIQDLFGTNLTTRIHFRPPCLLYYQIDFSQLAGVLKKRPII----RLLSKIGLAPKITKPVKSGMLDGHKFYLVVYAVPSAPGQSYIITRFVASAGTRWRNVKSLM--RWDDHLENMAVTDGDNMHVHYQERRLREHE-NGLEKFDRAYYMPAPADTAVRSFRRWLMNDGGGKPSW-KGSTGQLGPLMTDRKKLFDRWESHTKYCPICRKAYAITRVLRSLTTVLSIGLGLDALTSVAAMAFLFP-PGGLLSRFLMSCMGGAALWGVPLLVSGVCGYGAYRLHGLERRWVYTDYNHVER 589          

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|298709173|emb|CBJ31116.1| (Pheophorbide a oxygenase, putative chloroplast precursor [Ectocarpus siliculosus])

HSP 1 Score: 319.316 bits (817), Expect = 5.909e-99
Identity = 196/505 (38.81%), Postives = 267/505 (52.87%), Query Frame = 0

Query:   92 PGHGSGAPIDWWRQWYPMAFLDDL---DPARPTQVELLGKLFVIWAD-HDKTWHAFEDQCPHRLAPLSEGRID-ERGNLQCAYHGWSFSGQGGCVNIPQAEPEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELH-DPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNY---VGKEGVFLSLPRLANST---IEFHAPTRLHYLFDFTG-----------FFQRVPLMKWAFRLEKWLRRLPPDTRPRSS-----LVTYTIPVGPGRSRILALFPRNFFAINK--PRWL------DHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAFYMPTKSDEGVRAFRQWLVRWGAGGPRWADGVDSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSLVCM-TAIWTPR---PASVLGKILG-AGVAMAFGSWQMARLEKRMLFIDYVHAHK 555
            P     A   W RQWYP+A + DL   DP  P  V+L+G+  V+W D  D  W AF D+CPHR APLSEGR+D E G LQC YHGW F G G C +IPQA P  +  A+ S RACA A+P  V +G +WLWPD SP+G++ S +  P+TVP L        G W  R L YG+DTL+EN+ DPAHVPFAHHGV   R+M  P+ + ++     G++         A S    + F AP  ++Y  D+T             F+ +       +++K +     D + RS       V Y +P  PG+  I     RNFF  +   PR        +HL ++ VLDGD   LH QER +     +  E  ++ +YMPT SD  VR FR+W    G  GP WA GVD A       R  ++DR   HT  CSAC  A R    +K ++A  +LV +  A   PR   P  ++G  L  AG+A+A     M++ +++ +F+DYVHA +
Sbjct:   32 PDTSKQAGFQWRRQWYPVAVMRDLEARDPRMPYPVQLMGEGLVVWKDPKDGGWKAFADRCPHRWAPLSEGRVDQESGRLQCIYHGWEFEGDGKCGSIPQATPGSRETAQNSKRACATAIPTKVVEGKLWLWPDPSPEGVKESETVPPATVPGLDFGSGDFGGNWYCRKLAYGFDTLIENLADPAHVPFAHHGVMGSRAMGTPMAIEMDKEAGSGEDEFVTKRTGYAGSNVMRVGFKAPGLIYYESDYTETLGTTVKRLAPLFRAIYWFMIKLKIDKRISMKDEDRKERSDRLLSYFVGYAVPTSPGKCMIFTRTARNFFLQHPVIPRRFRNSIAKEHLGQHLVLDGDSPALHIQERLLADAGERGVERPEKTYYMPTASDVPVRFFRKWYHSRGGAGPPWAKGVDPADLGPILPREEVLDRMDAHTKDCSACSKAFRVSGAVKRLSAGSTLVLLGAAAAVPRGLLPVGLVGGALASAGLAVA-----MSKRQQQFVFLDYVHAER 531          

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|1029133956|ref|XP_016745156.1| (PREDICTED: pheophorbide a oxygenase, chloroplastic-like [Gossypium hirsutum])

HSP 1 Score: 314.694 bits (805), Expect = 3.788e-97
Identity = 198/579 (34.20%), Postives = 291/579 (50.26%), Query Frame = 0

Query:    5 MFLRLLVVVGTIASPITAFFTSSG-----SPLFRCVLPAKTSPPQPPHAVGPFATVAQPAPTPVSPMPPSSTTSSSSLTNAPSSSGSLQPLGPGHGSGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWADHDKT-WHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAE---PEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHDPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEGVFLSLPRLANSTIEFHAPTRLHYLFDFTGFFQRVPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAIN---------KPRWLDHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAF----YMPTKSDEGVRAFRQWLVRWGAGGPRWADGV-DSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNI----RVLKGVTAAMSLVCMTAIWTPRPASVLGKILGA--GVAMAFGSWQMARLEKRMLFIDYVHAH 554
            +FL   V    IAS   A   +S      SP    + P + +  +PPH   P + VA        P P         +++  S             S A   W   WYP++ ++DLDP  PT  +LLG+  V+W D  K  W AF+D+CPHRLAPLSEGR+DE G+LQC+YHGWSF G G C  IPQA    PE +AV  +S RACA   P +V+QG++++WPD   +G E + +  P  +P+           + RDL YGYDTL+ENV DP+H+ FAHH V  +R  A PL   ++  G  G   S       + +F AP    Y  +      ++P++      +KW          +  + ++ +P+ PG++R +    RNFF             PRW +H   N V DGD++ L GQE+      ++  E+I++ +    + PT++D  V AFR WL R G G P W   V    LP     +  ++DR+ QHT +CS+C+ A ++     ++L G T A    C TA     P+ +  +++ A  GV  A  ++ +  LEK  +F+DYVHA 
Sbjct:    3 VFLATTVSTTRIASSFIASSQTSKPQAFISPTKNNLAPRRITRLKPPHVAAPSSAVA--------PSPDQKQDEEYRVSDEFSE----------ESSDAKFSWKDHWYPVSLVEDLDPTLPTPFQLLGRDLVLWFDKSKNQWVAFDDKCPHRLAPLSEGRLDENGHLQCSYHGWSFDGCGSCTRIPQAASQGPEARAV--QSPRACATVFPTMVSQGLLFVWPDE--NGQERASATKPPMLPDDFKKPEFATVNIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVDSSGPWGFGGSNDDNPKISSKFIAPC---YYINKIEIDTKLPIVG----EQKW----------KIWICSFNVPMAPGKTRSIVCSARNFFQFTVPGSAWWQVVPRWFEHWTSNKVYDGDMIVLQGQEKIFLSKSMESSEDINKQYTKLTFTPTQADRFVLAFRNWLRRHGNGQPEWLTSVGQQPLPSTVLSKRQMLDRFEQHTLKCSSCKQAYKSFQTWQKILMGATVAF---CATA---SIPSDIQFRVVLALLGVVSAGLAYTLHELEKNFVFVDYVHAE 536          

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|823123477|ref|XP_012475848.1| (PREDICTED: pheophorbide a oxygenase, chloroplastic [Gossypium raimondii] >gi|763741490|gb|KJB08989.1| hypothetical protein B456_001G117200 [Gossypium raimondii])

HSP 1 Score: 313.923 bits (803), Expect = 6.676e-97
Identity = 198/579 (34.20%), Postives = 291/579 (50.26%), Query Frame = 0

Query:    5 MFLRLLVVVGTIASPITAFFTSSG-----SPLFRCVLPAKTSPPQPPHAVGPFATVAQPAPTPVSPMPPSSTTSSSSLTNAPSSSGSLQPLGPGHGSGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWADHDKT-WHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAE---PEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHDPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEGVFLSLPRLANSTIEFHAPTRLHYLFDFTGFFQRVPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAIN---------KPRWLDHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAF----YMPTKSDEGVRAFRQWLVRWGAGGPRWADGV-DSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNI----RVLKGVTAAMSLVCMTAIWTPRPASVLGKILGA--GVAMAFGSWQMARLEKRMLFIDYVHAH 554
            +FL   V    IAS   A   +S      SP    + P + +  +PPH   P + VA        P P         +++  S             S A   W   WYP++ ++DLDP  PT  +LLG+  V+W D  K  W AF+D+CPHRLAPLSEGR+DE G+LQC+YHGWSF G G C  IPQA    PE +AV  +S RACA   P +V+QG++++WPD   +G E + +  P  +P+           + RDL YGYDTL+ENV DP+H+ FAHH V  +R  A PL   ++  G  G   S       + +F AP    Y  +      ++P++      +KW          +  + ++ +P+ PG++R +    RNFF             PRW +H   N V DGD++ L GQE+      ++  E+I++ +    + PT++D  V AFR WL R G G P W   V    LP     +  ++DR+ QHT +CS+C+ A ++     ++L G T A    C TA     P+ +  +++ A  GV  A  ++ +  LEK  +F+DYVHA 
Sbjct:    3 VFLATSVSTTRIASSFIASSQTSKPQAFISPTKNNLAPRRITRLKPPHVAAPSSAVA--------PSPDQKQDEEYRVSDEFSE----------ESSDAKFSWKDHWYPVSLVEDLDPTLPTPFQLLGRDLVLWFDKSKNQWVAFDDKCPHRLAPLSEGRLDENGHLQCSYHGWSFDGCGSCTRIPQAASQGPEARAV--QSPRACATVFPTMVSQGLLFVWPDE--NGQERASATKPPMLPDDFKKPEFATVNIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVDSSGPWGFGGSNDDNPKISSKFIAPC---YYINKIEIDTKLPIVG----EQKW----------KIWICSFNVPMAPGKTRSIVCSARNFFQFTVPGSAWWQVVPRWFEHWTSNKVYDGDMIVLQGQEKIFLSKSMESSEDINKQYTKLTFTPTQADRFVLAFRNWLRRHGNGQPEWLTSVGQQPLPSTVLSKRQMLDRFEQHTLKCSSCKQAYKSFQTWQKILMGATVAF---CATA---SIPSDIQFRVVLALLGVVSAGLAYTLHELEKNFVFVDYVHAE 536          

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|470116673|ref|XP_004294502.1| (PREDICTED: pheophorbide a oxygenase, chloroplastic [Fragaria vesca subsp. vesca])

HSP 1 Score: 312.768 bits (800), Expect = 2.675e-96
Identity = 189/546 (34.62%), Postives = 277/546 (50.73%), Query Frame = 0

Query:   24 FTSSGSPLFRCVLPAKTSPPQPPHAVGPFATVAQPAPTPVSPMPPSSTTSSSSLTNAPSSSGSLQPLGPGHGSGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWADHDK-TWHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAEPEI-KAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHDPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEGVFLSLPRLANSTIEFHAPTRLHYLFDFTGFFQRVPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAINK---------PRWLDHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAF----YMPTKSDEGVRAFRQWLVRWGAGGPRWADGVDSA-LPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSLVCMTAIWTPRPASVLGKILGAGVAMAFGSWQMARLEKRMLFIDYVHA 553
            FTS  S   +  LP   S   P     P     + +P  V+  P ++     + T     S +L+       SG   +W   WYP++ ++DLDP  PT  +LLG+  V+W D +K  W A +D+CPHRLAPLSEGRIDE GNLQC+YHGWSF G G CV IPQA P+  +A A RS RACA   P LV+QG++++WPD   +G E + +  P  +P+           + RDL YGYDTL+ENV DP+H+ FAHH V  +R  A PL   L   G  G   +       T EF AP    Y  +      ++PL+      +KW+            + ++ +P+ PG++R +    RNFF  +          PRW +H   N V DGD++ L GQE+       +   ++++ +    + PT++D  V AFR WL R G   P W    D   LP     +  ++DR+ QHT +CS+C+GA    +  + +    ++V  +    P    +   + G  +  A  ++ +  LEK  +F+DYVHA
Sbjct:   14 FTSPQSQSLKPSLPFLISTVNPHRK--PSGRSKRFSPLRVATQPQTTDLGQPTKTKQDGESYNLENEVEDESSGTKFNWRDHWYPVSLIEDLDPKLPTPFQLLGRDIVLWFDRNKQEWVALDDKCPHRLAPLSEGRIDEGGNLQCSYHGWSFDGCGSCVKIPQASPQGPEARAVRSPRACATRFPTLVSQGLLFVWPDE--NGWERANASKPPMLPDEFGKPEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLEASGPWGFSGANEGNPRITAEFIAPC---YYLNKIEIDTKLPLVG----DQKWV----------IWICSFNVPMAPGKTRSIVCSARNFFQFSMPGPAWWQVIPRWHEHWTSNKVYDGDMIVLQGQEKVFLSKSKEEFGDVNKQYTTITFTPTQADRFVLAFRNWLRRHGNSQPEWFGTSDQQPLPSTILSKRQMLDRFEQHTLKCSSCKGAYTAFQTWQKLLTGAAVVFTSTAGIPSDLKLRIILAGFALVSAGLAYALHELEKNFVFVDYVHA 538          

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|670361997|ref|XP_008651038.1| (pheophorbide a oxygenase, chloroplastic [Zea mays] >gi|1142624553|gb|ONL93615.1| Lethal leaf-spot 1 [Zea mays])

HSP 1 Score: 310.842 bits (795), Expect = 6.148e-96
Identity = 180/475 (37.89%), Postives = 254/475 (53.47%), Query Frame = 0

Query:   96 SGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWAD-HDKTWHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAEPEI-KAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVP-ELHDPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEGVFLSLPRLANSTIEFHAPTRLHYLFDFTGFFQRVPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAINK---------PRWLDHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAF----YMPTKSDEGVRAFRQWLVRWGAGGPRW-ADGVDSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSLVCMTAIWTPRPASVLGKILGAGVAMAFGSWQMARLEKRMLFIDYVHA 553
            SG    W   WYP++ ++DLDP+RPT  +LL +  VIW +     W A +D+CPHRLAPLSEGRIDE G LQC+YHGWSF G G C  IPQA PE  +A A RS +ACA   P LV+QG++++WPD   +G E + +  P  +P E  DPAF     + RDL YGYDTL+ENV DP+H+ FAHH V  +R  A PL   +   G  G   +       T  F AP    Y  +      ++P+    F  +KW+            + ++ IP+ PG++R +    RNFF             PRW +H   N V DGD++ L GQE+       +   +I++ +    + PT++D  V AFR WL ++G   P W  +    ALP     +  ++DRY QHT +CS+C+GA    + L+ V    ++VC  A   P    +   I  A +  A  ++    L+K  +F+DYVHA
Sbjct:   63 SGEKFSWRDHWYPVSLVEDLDPSRPTPFQLLNRDLVIWKEPKSGEWVALDDRCPHRLAPLSEGRIDETGCLQCSYHGWSFDGSGACTKIPQAMPEGPEARAVRSPKACAIKFPTLVSQGLLFVWPDE--NGWEKAAATKPPMLPKEFEDPAF-STVTIQRDLFYGYDTLMENVSDPSHIEFAHHKVTGRRDRARPLTFRMESSGAWGYSGANSGNPRITATFEAPC---YALNKIEIDTKLPI----FGDQKWV----------IWICSFNIPMAPGKTRSIVCSARNFFQFTMPGKAWWQLVPRWYEHWTSNLVYDGDMIVLQGQEKIFLAATKESSTDINQQYTKITFTPTQADRFVLAFRTWLRKFGNSQPEWFGNPTQEALPSTVLSKREMLDRYEQHTLKCSSCKGAYNAFQNLQKVFMGATVVCCAAAGIPPDVQLRLLIGAAALVSAAIAYAFHELQKNFVFVDYVHA 517          

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|729327884|ref|XP_010535635.1| (PREDICTED: pheophorbide a oxygenase, chloroplastic [Tarenaya hassleriana])

HSP 1 Score: 311.227 bits (796), Expect = 8.129e-96
Identity = 180/476 (37.82%), Postives = 262/476 (55.04%), Query Frame = 0

Query:  102 WWRQWYPMAFLDDLDPARPTQVELLGKLFVIWAD-HDKTWHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAE---PEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHDPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGK---EGVFLSLPRLANSTIEFHAPTRLHYLFDFTGFFQRVPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAIN---------KPRWLDHLERNAVLDGDLVFLHGQE-----REMRRPELKWEENIDRAFYMPTKSDEGVRAFRQWLVRWGAGGPRWADGV-DSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSLVCMTAIWTPRPASVLGKILGAGVAM--AFGSWQMARLEKRMLFIDYVHA 553
            W   WYP++ ++DLDP  PT+ +LLG+  V+W D +D+ W AF+D CPHRLAPLSEGRIDE G+LQC+YHGWSF G G C  IPQA    PE +AV  +S RACA   P +V+QG++++WPD   +G + + S  P  +P+  D        + RDL YGYDTL+ENV DP+H+ FAHH V  +R  A PL   L   G    EG     P++   + +F AP    Y  +      ++P++      +KW+            + ++ +P+ PG++R +    RNFF  +          PRW +H   N V DGD++ L GQE     + M  P+    +   +  + PT++D  V AFR WL R G   P W   V + +LP     +  ++DR+ QHT  CS+C+GA    R  + +    + VC  AI    P+ V  +I+ AG+A+  A  ++ +  LEK  +F+DYVHA
Sbjct:   89 WRDHWYPVSLVEDLDPDLPTRFQLLGRDLVLWFDRNDQNWVAFDDLCPHRLAPLSEGRIDENGHLQCSYHGWSFDGCGSCTRIPQASSSGPEARAV--KSPRACAIRFPTMVSQGLLFVWPDE--NGWDRANSTEPPRLPDDFDKPEFATVSIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLEASGPWGFEGANDDNPKI---SAKFVAPC---YYLNKIEIDTKLPVVG----DQKWV----------IWICSFNVPMAPGKTRSIVCSARNFFQFSVPGPAWWQVVPRWYEHWTSNKVYDGDMIVLQGQEKVFLSKTMESPDNDVNKQYTKLTFTPTQADRFVLAFRNWLRRHGKSQPEWFGTVTNQSLPSTVLTKRQMLDRFEQHTQVCSSCKGAYEGFRTFQKILVGTT-VCFAAI-AGVPSEVQFRIILAGLALISAASAYALHELEKNFVFVDYVHA 538          

BLAST of EWM26188.1 vs. NCBI_GenBank
Match: gi|1208516024|gb|OWM73739.1| (hypothetical protein CDL15_Pgr026843 [Punica granatum])

HSP 1 Score: 311.612 bits (797), Expect = 9.843e-96
Identity = 178/490 (36.33%), Postives = 257/490 (52.45%), Query Frame = 0

Query:   83 SSGSLQPLGPGHGSGAPIDWWRQWYPMAFLDDLDPARPTQVELLGKLFVIWADHDKT-WHAFEDQCPHRLAPLSEGRIDERGNLQCAYHGWSFSGQGGCVNIPQAE---PEIKAVAERSGRACAKAVPLLVAQGMVWLWPDNSPDGMEASRSQLPSTVPELHDPAFIRGAWVTRDLMYGYDTLVENVVDPAHVPFAHHGVQAQRSMAMPLNVSLNYVGKEGVFLSLPRLANSTIEFHAPTRLHYLFDFTGFFQRVPLMKWAFRLEKWLRRLPPDTRPRSSLVTYTIPVGPGRSRILALFPRNFFAINK---------PRWLDHLERNAVLDGDLVFLHGQEREMRRPELKWEENIDRAF----YMPTKSDEGVRAFRQWLVRWGAGGPRW-ADGVDSALPPRSNDRHFLMDRYTQHTTQCSACRGALRNIRVLKGVTAAMSLVCMTAIWTPRPASVLGKILGAGVAMAFGSWQMARLEKRMLFIDYVHAH 554
            S G  + LG    S +   W   WYP++ ++DLDP  PT  +LLG+  V+W D   + W AF+D+CPHRLAPLSEGRIDE GNLQC+YHGWSF G G C  IPQA    PE +AV  RS RACA   P +V+QG++++WPD   +G E + +  P+ +P+  D        + RDL YGYDTL+ENV DP+H+ FAHH V  +R  A PL   +   G  G   +       + +F AP    Y  +      ++P++      +KW+            + ++ +P+ PG++R +    RNFF             PRW +H   N V DGD++ L GQE+       +  E++++ +    + PT++D  V AFR WL R G   P W        LP     +  ++DR+ QHT +CS+C+GA    R  + V    ++VC      P        + G GV  A  ++ +  L+K  +FIDYVHA 
Sbjct:   82 SIGVEEQLGE-ESSSSKFSWRDHWYPVSLVEDLDPRLPTPFQLLGRDTVLWFDKSSSQWVAFDDRCPHRLAPLSEGRIDEDGNLQCSYHGWSFDGCGSCTRIPQAASDGPEARAV--RSPRACATRFPTMVSQGLLFVWPDE--NGFERAAATKPTMLPDDFDNPEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVESSGPWGFAGANEDHPKISAKFVAPC---YYMNKIEIDTKLPIIG----DQKWI----------IWICSFNVPMAPGKTRSIVCSARNFFQFTMPGPAWWQVIPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKSAEGLEDVNKEYTKITFTPTQADRFVLAFRNWLRRHGNSEPEWFGSSKQQPLPSTVLSKRQMLDRFEQHTLKCSSCKGAYEAFRTWQKVLIGATVVCCAVAGIPSEIQFRILLAGLGVVSAGIAYALHELQKNFVFIDYVHAE 549