EWM26223.1, cds4466 (CDS) Nannochloropsis gaditana

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|585108222|gb|EWM26223.1| (isocitrate lyase [Nannochloropsis gaditana])

HSP 1 Score: 1264.21 bits (3270), Expect = 0.000e+0
Identity = 607/607 (100.00%), Postives = 607/607 (100.00%), Query Frame = 0

Query:    1 MYWKRTCCDCLGLHFRTLILDLFPCSAGALHSNPYRLSVRKPIKITITTRTMEETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKAQAYNLSLPQARALAKELMGREVYFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAKGPGAAGVSSGAGAARAGAISRL 607
            MYWKRTCCDCLGLHFRTLILDLFPCSAGALHSNPYRLSVRKPIKITITTRTMEETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKAQAYNLSLPQARALAKELMGREVYFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAKGPGAAGVSSGAGAARAGAISRL
Sbjct:    1 MYWKRTCCDCLGLHFRTLILDLFPCSAGALHSNPYRLSVRKPIKITITTRTMEETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKAQAYNLSLPQARALAKELMGREVYFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAKGPGAAGVSSGAGAARAGAISRL 607          

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|298706764|emb|CBJ29687.1| (Isocitrate lyase (Isocitrase) (Isocitratase) (ICL) [Ectocarpus siliculosus])

HSP 1 Score: 739.954 bits (1909), Expect = 0.000e+0
Identity = 347/533 (65.10%), Postives = 428/533 (80.30%), Query Frame = 0

Query:   54 ETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALST---LPNPAPKLAVWKAQAYNLSLPQARALAKELM-GREVYFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQF 582
            + + ++ DV AT+ ++ +PRF  T RP+SA++VV+LR +L     S+ QA+KLW LL      G  S+TFGALDPVQV++MAPH+ T+YVSGWQCSSTASTSNEPGPDFADYP DTVPNKV QLF AQ  HDRRQ + R  M++  +++ P VDYLRPIIADGDTGHGGLTAVMKLTKMFIE+GAAGIHFEDQKPGTKKCGHMGGKVLVS+QEH DRL AARLQAD+MG +T+IVARTD EAASLLD N+D RDHPFI+GATVPGTR LN++V EARA G +   +D+++  W   A L RFPEAV  A+ +   L     +L  WK++AY+LS  +AR L+  L+ G++VYFDW+APRSREGY+++KGGVDYC+AR  AYA HADL+WMETAKP+L EAR FA+G+ AAVP + LAYNLSPSFNWD AG+S QEM  FN  L  +GFVWQFITLAGFH++GL+ +  A+EYGK GV+ YVE++QR ER ++VD+LTHQKWSGA L+DKQ+   TGG++STS+MG+GVTE+QF
Sbjct:   24 QAKTFNHDVEATKRWWGSPRFRYTKRPFSAEEVVSLRGTLPETYPSDIQARKLWELLEKKHALGDFSHTFGALDPVQVIEMAPHLETVYVSGWQCSSTASTSNEPGPDFADYPYDTVPNKVDQLFRAQRHHDRRQNDERCSMSEGEQLSAPAVDYLRPIIADGDTGHGGLTAVMKLTKMFIEKGAAGIHFEDQKPGTKKCGHMGGKVLVSMQEHCDRLVAARLQADIMGTETVIVARTDAEAASLLDNNVDPRDHPFIVGATVPGTRPLNDLVREARAGGASFEAIDKLSKDWGEKAGLMRFPEAVEAAIHSMRGLQGRDQRLQEWKSKAYSLSNAEARVLSARLLGGKQVYFDWDAPRSREGYFKLKGGVDYCIARGRAYAQHADLLWMETAKPNLPEARAFAEGIHAAVPHQKLAYNLSPSFNWDAAGMSKQEMAAFNRELGRLGFVWQFITLAGFHSDGLIVSRLAKEYGKHGVIKYVELVQRPERNEQVDILTHQKWSGANLMDKQVNIATGGLASTSAMGEGVTESQF 556          

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|909136696|gb|KNE62501.1| (isocitrate lyase [Allomyces macrogynus ATCC 38327])

HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0
Identity = 313/537 (58.29%), Postives = 396/537 (73.74%), Query Frame = 0

Query:   53 EETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGT--RALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDAL--STLPNPAPKLAVWKAQAYNLSLPQARALAKELMGREVYFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAK 585
            EE R + E+VAA + ++ +PRFA+T RPY+A+DVVA R ++ +E  SN+QA+KLW+LL      G  S+TFGALDP+QV QMA ++ T+YVSGWQCSSTASTSNEPGPD ADYPMDTVPNKV  LF AQLFHDR+Q+  R     A +  EP +D+LRPIIADGDTGHGG+TA MKL KMF+ERGAAG+H EDQ PGTKKCGHM GKVLV + EHI+RL A RLQ D+MG  T++VARTD EAA+LL  NID RDH FILG + P    ++L E++ EA+  G  GA L ++   W   A L+ + EAV DA+  S+  + A  +A +  +   +S  +ARA AK +   +V++ W+ PR REG++R +GG   CV RA+AYAP+ DLIWMETAKP L++AREF+QGV +A P  MLAYNLSPSFNWD AG+S ++M  F  +L  +GF WQFITLAGFH + L    FAR+Y KRG++AYVE IQR+ERE+ V+ L HQKWSGA  +D  M TV GG+S+T++MGKGVTE QFG K
Sbjct:   10 EEERRFQEEVAAVKKWWSSPRFARTTRPYTAEDVVAKRGTIHIEYPSNQQAKKLWALLQNHKRNGTASHTFGALDPIQVAQMAKYLETVYVSGWQCSSTASTSNEPGPDLADYPMDTVPNKVDHLFRAQLFHDRKQRSQRLSAPKAQRANEPAIDFLRPIIADGDTGHGGITANMKLAKMFVERGAAGVHVEDQSPGTKKCGHMAGKVLVPISEHINRLIAMRLQFDIMGTDTLLVARTDAEAATLLSNNIDPRDHQFILGCSNPALKGKSLVELMDEAQRAGKQGAALAQVESDWVERAQLKTYTEAVIDAIKASSRADKAQLIAAFTKKTQGVSNSEARAAAKAIGFGDVFWCWDLPRVREGFWRYRGGTAACVVRAIAYAPYTDLIWMETAKPILAQAREFSQGVLSADPTVMLAYNLSPSFNWDAAGMSDEDMRTFIVNLGKLGFTWQFITLAGFHCDALGIDTFARDYAKRGMLAYVEGIQRQERERGVETLAHQKWSGAGYIDSMMMTVQGGVSATAAMGKGVTEDQFGKK 546          

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|914266244|gb|KNZ80971.1| (Isocitrate lyase [Termitomyces sp. J132])

HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0
Identity = 310/535 (57.94%), Postives = 398/535 (74.39%), Query Frame = 0

Query:   53 EETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKAQAYNLSLPQARALAKELMG-REV-YFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAK 585
             E   + ++VA  E +++NPRFA+  RPY+A +VVA R +L ++ ASN  A+KLW+LL   A +G  S+T+GALDPVQV QMA ++ T+YVSGWQ SSTAS++NEPGPD ADYP +TVPNKV  LF AQLFHDR+Q+EAR+ MTDA     P VDYLRPI+AD DTGHGGLTAVMKLTKMF+E+GAAGIH EDQ PGTKKCGHM GKVLV +QEHI+RL A RLQ D+MG + + +ARTD EAA+L+ +NID RDH FILG+T P    LN V+  A   G++GAEL    D+W  AA L+ F E + DAL+     +  +  + A+  ++S P A ++A++  G R V ++DW+APR+REGYYR +GG    V RA+A+AP+ADL+WMET KP L++A+EFA GV A  PG+ LAYNLSPSFNWD AGL  ++ME +   L  +GFVWQFITL G H+N  ++ +FA+ + K G+ AYVE++QRKER+   D+LTHQKWSGA   D  ++TVTGG+SSTS+MGKGVTEAQF  K
Sbjct:    3 NERAAFAKEVAEVERWWKNPRFARVKRPYTAAEVVAKRGTLAIQYASNVMAKKLWALLNEHAQRGTPSHTYGALDPVQVTQMAKYLETVYVSGWQSSSTASSTNEPGPDLADYPSNTVPNKVEHLFMAQLFHDRKQREARSSMTDAELSKTPIVDYLRPIVADADTGHGGLTAVMKLTKMFVEKGAAGIHIEDQAPGTKKCGHMAGKVLVPIQEHINRLVAIRLQFDIMGVENLAIARTDSEAATLITSNIDDRDHAFILGSTNPNLAPLNTVMVNAEKAGMSGAELQATEDEWIQAAKLKLFSETLADALAKQGANSSIVKNFIARVAHISYPAAVSIAQKEFGLRNVPFWDWDAPRTREGYYRYQGGTQCAVNRAIAFAPYADLLWMETKKPILAQAQEFAAGVHAVHPGQWLAYNLSPSFNWDAAGLGEKDMEAYVWQLGKLGFVWQFITLGGLHSNAYISDLFAQNFAKTGMKAYVELVQRKERDLGTDVLTHQKWSGADYADNLIKTVTGGVSSTSAMGKGVTEAQFTTK 537          

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|648169061|gb|KDR82701.1| (hypothetical protein GALMADRAFT_238193 [Galerina marginata CBS 339.88])

HSP 1 Score: 646.736 bits (1667), Expect = 0.000e+0
Identity = 307/535 (57.38%), Postives = 399/535 (74.58%), Query Frame = 0

Query:   53 EETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKAQAYNLSLPQARALAKELMGREV--YFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAK 585
            E+ R + ++VA  E +++NPRFA+ +RPY+A DVV+ R +L ++  SN Q +KLWS+L+  A +G  S+T+GALDPVQV QMA ++ T+YVSGWQ SSTAS++NEPGPD ADYP +TVPNKV  LF AQLFHDR+Q+EAR+R++ A     P +DYLRPI+AD DTGHGGLTAVMKLTKMF+E+GAAGIH EDQ PGTKKCGHM GKVLV +QEHI+RL A RLQ D+MG + + +ARTD EAA+L+ +NIDARDH FILG+T    R LN V+ EA A G  G +L  I DQW A+ANL+ F   + +ALS        +  + A+  + S P A  +A+   G +   Y+DW++PR+REGYYR +GG    + RA+A+AP+ADL+WMET KP LS+AREFA GV AA PG  LAYNLSPSFNW+ AGL+ ++M+ +   L  +GFVWQFITL G H+N  ++ +FA+ + K G+ AYVE++QR+ERE   D+LTHQKWSGA   D  ++TVTGG+SSTS+MG GVTE+QF AK
Sbjct:    4 EQAR-FADEVAEVEQWWKNPRFARVIRPYTAADVVSKRGTLPIQYPSNVQGKKLWSVLSEHAKRGTPSHTYGALDPVQVTQMAKYLETVYVSGWQSSSTASSTNEPGPDLADYPSNTVPNKVEHLFMAQLFHDRKQREARSRLSAAELAKTPNIDYLRPIVADADTGHGGLTAVMKLTKMFVEKGAAGIHVEDQAPGTKKCGHMAGKVLVPIQEHINRLVAIRLQYDIMGVENLAIARTDSEAATLITSNIDARDHAFILGSTNASLRPLNAVMVEAEALGKNGNQLQVIEDQWIASANLQLFSTTLANALSKQGVNKSTVVKFVARIASSSYPDAVTIAQNEFGLKSVPYWDWDSPRTREGYYRYQGGTQCAIHRAIAFAPYADLLWMETKKPILSQAREFAHGVHAARPGHWLAYNLSPSFNWEAAGLTDKDMKDYVWELGKLGFVWQFITLGGLHSNAYISDLFAQNFAKTGMKAYVELVQRRERELGCDVLTHQKWSGADYADNLIKTVTGGVSSTSAMGAGVTESQFAAK 537          

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|302689573|ref|XP_003034466.1| (isocitrate lyase [Schizophyllum commune H4-8] >gi|300108161|gb|EFI99563.1| isocitrate lyase [Schizophyllum commune H4-8])

HSP 1 Score: 646.351 bits (1666), Expect = 0.000e+0
Identity = 304/534 (56.93%), Postives = 397/534 (74.34%), Query Frame = 0

Query:   54 ETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKAQAYNLSLPQARALAKELMGREV--YFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAK 585
            E   + ++VA  E ++++PRF +  RPY+A DVV+ R +L ++  SN Q +KLWS+L   A +G  S+T+GALDPVQV QMA ++ T+YVSGWQ SSTAS++NEPGPD ADYP +TVPNKV  LF AQLFHDR+Q+EAR+ ++D+   + P +DYLRPI+AD DTGHGGLTAVMKLTK+F+E+GAAGIH EDQ PGTKKCGHM GKVLV + EHI+RL A RLQ D++G + + +ARTD EAA+L+ +NIDARDH FILG+T P   ALN+V+  A  +G  G EL    D W A ANL+ F + + DAL+     A  +  + A+  + S P+A A+A++  G +   ++DW+APR+REG+YR +GG    + RAVAYAP+ADL+WMET KP L++A+EFA GV A  PG  LAYNLSPSFNWD AGL   +M+ +   L  +GFVWQFITL G H+N  ++ +FAREY K G+ AYVE++QRKERE   D+LTHQKWSGA   D  ++TVTGG+SST++MGKGVTE+QFGAK
Sbjct:    4 ERAAFAKEVAEVEQWWKSPRFERVKRPYTAADVVSKRGTLPIQYPSNVQGKKLWSILGEHAKRGTPSHTYGALDPVQVTQMAKYLETVYVSGWQSSSTASSTNEPGPDLADYPYNTVPNKVEHLFQAQLFHDRKQREARSHLSDSELTSTPSIDYLRPIVADADTGHGGLTAVMKLTKLFVEKGAAGIHIEDQAPGTKKCGHMAGKVLVPISEHINRLVAIRLQYDILGVENLAIARTDSEAATLITSNIDARDHAFILGSTNPSIGALNDVMLAAEREGKNGEELQATEDNWLAQANLQLFSKTLADALTAEGASAANVDKFLARVAHASYPEAVAVAQKEFGLKTVPFWDWDAPRTREGFYRYQGGTQCAINRAVAYAPYADLLWMETKKPILAQAKEFAAGVHAVHPGHWLAYNLSPSFNWDAAGLGEDDMQSYVWELGKLGFVWQFITLGGLHSNAYISDLFAREYAKTGMKAYVELVQRKEREIGTDVLTHQKWSGADYADNLLKTVTGGISSTAAMGKGVTESQFGAK 537          

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|1210528002|dbj|GAX13117.1| (isocitrate lyase [Fistulifera solaris])

HSP 1 Score: 645.58 bits (1664), Expect = 0.000e+0
Identity = 319/541 (58.96%), Postives = 399/541 (73.75%), Query Frame = 0

Query:   59 HEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSS------LLVEPA---SNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKAQAYN-----LSLPQARALAKELMGRE--VYFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFG 583
             +DVAA E ++++PR+  T R YSA DV  LR S       L  P    S++Q+ KLW LLT L   G  S+TFGALDPVQV QMAPH+S+IYVSGWQCSSTAST+NEPGPDFADYP +TVPNKV QL  AQL HDRRQQ+ R++   A +     +DYL+PI+ADGDTGHGGL+AVMKLTK+ IE GAAG+H EDQKPGTKKCGHMGGKVLVS QEHIDRL AARL AD+MGA  I+VARTD EAA+LLD+NID RDHPFILG T P   +L EV+A A  +     +  +++ +W A ANL  F EAV   +     PA +    K Q  +     LS   AR +A +++GR+  VYFDWE  R REGYY++K G+DYCV RA AY+P+ADLIWMETA P +S+A +F+ GVR+A P +MLAYNLSPSFNWD +G+S  E+  FN  L  +G+VWQFITLAGFH+NGL+ T  AR Y  RG++AY E+IQR+ER++++++L HQKWSGA L+D+Q+   +GG+SST++MG GVTE+QFG
Sbjct:   50 QQDVAAIEQWWQDPRWKHTKRTYSAMDVACLRPSHQARGGSLEAPGTSFSSQQSMKLWDLLTKLHEVGGYSHTFGALDPVQVTQMAPHLSSIYVSGWQCSSTASTTNEPGPDFADYPSNTVPNKVDQLVKAQLHHDRRQQQERSQALLAGQDPGTKLDYLKPIVADGDTGHGGLSAVMKLTKLMIEAGAAGMHLEDQKPGTKKCGHMGGKVLVSTQEHIDRLVAARLAADIMGANLILVARTDAEAATLLDSNIDGRDHPFILGVTDPSLPSLQEVLASAPPE-----QAAQVSAEWKAKANLMTFSEAVLSEIERSAAPAYRKRQMKDQWMSADPDILSNAAARRIADQILGRKNAVYFDWEKCRVREGYYQLKAGIDYCVQRARAYSPYADLIWMETAIPAVSDAAKFSHGVRSACPHQMLAYNLSPSFNWDASGMSDAEISRFNDDLGRLGYVWQFITLAGFHSNGLIITELARAYADRGMIAYCELIQRQERKKQIELLKHQKWSGAELIDQQVIVASGGVSSTAAMGSGVTESQFG 585          

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|1012964700|gb|KYQ39454.1| (Isocitrate lyase [Hypsizygus marmoreus])

HSP 1 Score: 645.195 bits (1663), Expect = 0.000e+0
Identity = 307/535 (57.38%), Postives = 397/535 (74.21%), Query Frame = 0

Query:   53 EETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKAQAYNLSLPQARALAKELMGREV--YFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAK 585
             E +++ ++VA  E ++++PRFA+  RPY+A +VVA R +L ++  SN QA+KLWSLL     +G  S+T+GALDPVQV QMA ++ T+YVSGWQ SSTAS++NEPGPD ADYP +TVPNKV  LF AQLFHDR+Q+EAR+ +++A     P +DYLRPI+AD DTGHGGLTA+MKLTKMF+E+GAAGIH EDQ PGTKKCGHM GKVLV +QEHI+RL A RLQ D+MG   + +ARTD EAA+L+ +NID RDHPFILG+T      LN V+ EA   G  G +L  I D W  AANL+ F  A+ DAL+     A  +  + A+  ++S PQA A+A++  G +   Y++W++PR+REGY+R +GG    V RA A+AP+ADL+WMET  P LS+A+EFA GV AA PG  LAYNLSPSFNWD AGL  +EM+ +   L  +GFVWQFITLAG H+N  ++ +FA+ + K G+ AYVE+IQRKERE   D+LTHQKWSGA   D  ++TVTGG+SST++MG GVTE+QFGAK
Sbjct:    3 NERKIFAQEVAQVEQWWKSPRFARVKRPYTAAEVVAKRGTLPIQYPSNVQAKKLWSLLEQHHKRGTPSHTYGALDPVQVTQMAKYLETVYVSGWQSSSTASSTNEPGPDLADYPSNTVPNKVEHLFMAQLFHDRKQREARSSLSEAELAKTPYIDYLRPIVADADTGHGGLTAIMKLTKMFVEKGAAGIHVEDQAPGTKKCGHMAGKVLVPIQEHINRLVAIRLQYDIMGVDNLAIARTDSEAATLITSNIDDRDHPFILGSTNASLEPLNTVMVEAERAGKNGNQLQAIEDAWIKAANLQLFSTALADALTKQGANASIVGKFVARVAHVSYPQAVAIAQKEFGLKTVPYWNWDSPRTREGYFRYQGGTQCAVNRAAAFAPYADLLWMETKSPILSQAKEFAAGVHAAHPGHWLAYNLSPSFNWDAAGLGEREMQGYVWELGKLGFVWQFITLAGLHSNAYISDLFAKNFAKTGMKAYVELIQRKERELGTDVLTHQKWSGADYADNLIKTVTGGVSSTAAMGAGVTESQFGAK 537          

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|1183508313|gb|ORZ31584.1| (isocitrate lyase [Catenaria anguillulae PL171])

HSP 1 Score: 644.425 bits (1661), Expect = 0.000e+0
Identity = 315/538 (58.55%), Postives = 391/538 (72.68%), Query Frame = 0

Query:   53 EETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRA--LNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKA---QAYNLSLPQARALAKELMGREVYFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAK 585
            EE R +  +VAA + ++++ RF++T RPY+A+DVV  R +   E  SN QA+KLWSLL      G  S+TFGALDP+QV QMA ++ T+YVSGWQCSSTASTSNEPGPD ADYPMDTVPNKV QLF AQ FHDR+Q+ AR  +    + + P VD+LRPIIAD DTGHGG+TA M+L KMF+ERGAAGIH EDQ  GTKKCGHM GKVLV + EHI+RL A RLQ D+MG  TI+VARTD EAA+LL  NID RDH FILG + P  R   L  ++ +A+  G  GA L ++ + W   A L+ + EA+ DA+    N A K  + K    +    S  +ARALAK+L   +V++ W+ PR REGYYR +GG   C+ RA+AYAP+ DLIWMETAKP LS+AREFA GV AA P  MLAYNLSPSFNWD AG++ ++M  + ++L  +GFVWQFITLAGFH++ L   +FAR+Y KRG++AYVE IQRKERE  V+ L HQKWSGA  +D  M TV GG+SST++MGKGVTE QFGAK
Sbjct:   10 EEERQFQAEVAAVKQWWKSERFSRTTRPYTAEDVVTKRGTFKTEYPSNMQAKKLWSLLKKHKAAGTASHTFGALDPIQVTQMAKYLETVYVSGWQCSSTASTSNEPGPDLADYPMDTVPNKVDQLFRAQQFHDRKQRAARVSLPKNQRASLPAVDFLRPIIADADTGHGGITANMRLAKMFVERGAAGIHVEDQAAGTKKCGHMAGKVLVPISEHINRLIAMRLQFDIMGTDTILVARTDAEAATLLQNNIDPRDHAFILGCSNPALRGKPLVTMMDQAQRAGKVGAALAQVEEDWVNQAQLKTYSEAIVDAILK-SNRADKQQLAKEFADKTSRCSNAEARALAKQLGFGDVFWCWDLPRVREGYYRYRGGTKACINRAIAYAPYTDLIWMETAKPILSQAREFAHGVLAADPTAMLAYNLSPSFNWDAAGMTDEDMRTYVTNLGKLGFVWQFITLAGFHSDALGIDLFARDYAKRGMLAYVEGIQRKEREHGVETLAHQKWSGAGYIDSMMMTVQGGVSSTAAMGKGVTEDQFGAK 546          

BLAST of EWM26223.1 vs. NCBI_GenBank
Match: gi|170089369|ref|XP_001875907.1| (isocitrate lyase icl [Laccaria bicolor S238N-H82] >gi|164649167|gb|EDR13409.1| isocitrate lyase icl [Laccaria bicolor S238N-H82])

HSP 1 Score: 643.269 bits (1658), Expect = 0.000e+0
Identity = 307/535 (57.38%), Postives = 394/535 (73.64%), Query Frame = 0

Query:   53 EETRLYHEDVAATEHFFRNPRFAQTVRPYSAQDVVALRSSLLVEPASNRQAQKLWSLLTGLAGQGKCSYTFGALDPVQVVQMAPHVSTIYVSGWQCSSTASTSNEPGPDFADYPMDTVPNKVHQLFSAQLFHDRRQQEARARMTDASKVAEPPVDYLRPIIADGDTGHGGLTAVMKLTKMFIERGAAGIHFEDQKPGTKKCGHMGGKVLVSVQEHIDRLTAARLQADVMGAQTIIVARTDGEAASLLDTNIDARDHPFILGATVPGTRALNEVVAEARAQGVTGAELDRITDQWTAAANLRRFPEAVCDALSTLPNPAPKLAVWKAQAYNLSLPQARALAKELMGREV--YFDWEAPRSREGYYRIKGGVDYCVARAVAYAPHADLIWMETAKPDLSEAREFAQGVRAAVPGKMLAYNLSPSFNWDVAGLSPQEMEHFNSSLAAMGFVWQFITLAGFHANGLMTTMFAREYGKRGVVAYVEMIQRKEREQEVDMLTHQKWSGAALLDKQMQTVTGGMSSTSSMGKGVTEAQFGAK 585
            E  R  HE VA  E +++NPRFA+  RPY+A DVVA R +L ++  SN Q +KLW LL+  A +G  S+T+GALDPVQV QMA ++ T+YVSGWQ SSTAS++NEPGPD ADYP +TVPNKV  LF AQLFHDR+Q+EAR+ +++A     P VDYLRPI+AD DTGHGGLTAVMKLTKMF+E+GAAGIH EDQ PGTKKCGHM GKVLV +QEHI+RL A RLQ D+MG + + +ARTD EAA+L+ +NID RDH FILGAT P    LN+V+ EA   G +G +L  I DQW ++ANL  F   +  AL         L    A+   +S P+A A+A++  G     Y++W++PR+REGYYR +GG    + RA+A+AP+ADL+WMET KP LS+A+EFA GV AA PG  LAYNLSPSFNW+ AGL+ ++M+ +   L  +GFVWQFITL G H+N  ++ +FA+ + K G+ AYVE++QRKERE   D+LTHQKWSGA   D  ++TVTGG+SST++MG GVTE+QFG+K
Sbjct:    4 ERARFAHE-VAQVEQWWKNPRFARVKRPYTAADVVAKRGTLAIQYPSNVQGKKLWKLLSEHAKRGTPSHTYGALDPVQVTQMAKYLETVYVSGWQSSSTASSTNEPGPDLADYPSNTVPNKVEHLFMAQLFHDRKQREARSSLSEAELAKTPVVDYLRPIVADADTGHGGLTAVMKLTKMFVEKGAAGIHVEDQAPGTKKCGHMAGKVLVPIQEHINRLVAIRLQYDIMGVENLAIARTDSEAATLITSNIDGRDHAFILGATNPSQPPLNDVMVEAELAGKSGDQLQAIEDQWISSANLELFSTTLAKALGKQGATKAALDKLVARVSRVSHPEAVAIAQKEFGLRTIPYWNWDSPRTREGYYRYQGGTQCAIHRAIAFAPYADLLWMETKKPILSQAQEFAHGVHAARPGHWLAYNLSPSFNWEAAGLNDKDMQGYVWELGKLGFVWQFITLGGLHSNAYISDLFAQNFAKTGMKAYVELVQRKERELGTDVLTHQKWSGADYADNLIKTVTGGVSSTAAMGAGVTESQFGSK 537