EWM30334.1, cds444 (CDS) Nannochloropsis gaditana

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|585112993|gb|EWM30334.1| (ubiquitin-activating enzyme e1 [Nannochloropsis gaditana])

HSP 1 Score: 2551.93 bits (6613), Expect = 0.000e+0
Identity = 1240/1240 (100.00%), Postives = 1240/1240 (100.00%), Query Frame = 0

Query:    1 MQDIWIVGVGILPSKPVPSSSQELQLEAAKMLRSRQRDKLRPYLVLYLFLIHVLVLLEQQRIQASIVVDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGPLTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDETLLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATLDEILASGYQAPTEVVRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIECVDDAGDEVESPPLRYFFPHVKKRKSKGRRPLAHRWWGGVLKAISK 1240
            MQDIWIVGVGILPSKPVPSSSQELQLEAAKMLRSRQRDKLRPYLVLYLFLIHVLVLLEQQRIQASIVVDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGPLTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDETLLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATLDEILASGYQAPTEVVRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIECVDDAGDEVESPPLRYFFPHVKKRKSKGRRPLAHRWWGGVLKAISK
Sbjct:    1 MQDIWIVGVGILPSKPVPSSSQELQLEAAKMLRSRQRDKLRPYLVLYLFLIHVLVLLEQQRIQASIVVDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGPLTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDETLLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATLDEILASGYQAPTEVVRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIECVDDAGDEVESPPLRYFFPHVKKRKSKGRRPLAHRWWGGVLKAISK 1240          

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|1189383022|gb|OSX70404.1| (hypothetical protein BU14_0767s0002 [Porphyra umbilicalis])

HSP 1 Score: 695.271 bits (1793), Expect = 0.000e+0
Identity = 453/1160 (39.05%), Postives = 630/1160 (54.31%), Query Frame = 0

Query:   68 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYL--DETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRF-RQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVG-------PLTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDETLLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATL--DEILASGYQ-APTEVVRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNK--EEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIECVDDAGDEVESPPLRYFF 1212
            +DE LYSRQLYVLG  AQ+R+A+S++L+VG+ GVGVEI KN+ILAG +SVTL DP PA   DLA Q+YL  D  + G  RA  S   L+ LNP V + LLE       +VA+ ++A+   +  +V  +Q  A++ A++   R    KF++  A G FG IF D G  FTV+D +G++ ++ ++ +        + +VT  + +RHD   GD V F    TE  G T            L        +++ P  F++      V  + +Y  G +  Q+K P  + F+ L  A +    V  DF K  + +  T HA F+++  F+   GG LP PGSA  AA F + V++               AA SAA              LD +++E FART  G L+P+ + +GG AAQEVLKA +G F+P++Q+ + D  EVL        SP       P+E ECA  G +YDG  AVLG+ + K L     F+VGAGAIGCE+LKN A+MGVG        + +TD D IEKSNL+RQFLFR+ D+  PKS AAA AV+ +N DV + A   +VG ETE  FDD FW G   V NALDNV AR YVD RCVYY +PLL+SGT GT GN QVVVP +TESY SS DPPE SIP+CTLK+FPY IEH +QWARD+F+G FR  PE   D  A L     RG++           L+ Q    T+L  LE     L    P+ S   C+KW+   F E++ S+I+ LL   P D V  +  PFWSG +R+PTP+ F+ +D  HM F+ SAALL+A+V+ +      +      + A +  D +  +G + A TE   +E+   G     + ++ E +   +K +   G     G   T     P  FEKD D N HI  IT+ SNLRAANY +  ADR ++K IAG+IIPAIATTT+ V GL  +E+ KL +    + +    D  +  K   +G   + RR       L  ++N F NLA PFFA ++P+ A    +G  G  + +WD L+V   +D+TL   L     ++GL+++ V+  G  ILYA F +P      L   ++E+     K    A                ++ ++  E+ C D  G++VE P  RY F
Sbjct:   22 IDEDLYSRQLYVLGHEAQRRMASSSVLLVGVDGVGVEIAKNLILAGLKSVTLFDPMPAMTADLAAQFYLTPDHVAAGTPRAAASLPLLESLNPYVSVSLLEADGG--ASVADTVSAAASQYTAVVLVNQSLAAQTAVDAACRSADVKFMSVGAFGAFGRIFNDFGPAFTVSDVNGEQPRSVMISA--VTVDGDDVVVTCLDEQRHDFEAGDHVSF----TEVQGMTG-----------LNDGKPREIKVLGPFTFSLVGCREEVAAMGEYVRGGYATQVKMPSTIVFKSLPEAAEEPEYVIADFAK--MMQMGTTHAAFQAVDAFRATLGGALPTPGSAAHAAKFVEFVEA---------------AAGSAADD------------LDAKVLECFARTAAGVLSPMAATLGGIAAQEVLKATSGKFMPIKQFFYIDCQEVL-------PSPP------PTEAECAPTGSRYDGQVAVLGATVQKKLAALNYFLVGAGAIGCEMLKNWAMMGVGVDAASGGGIVITDMDQIEKSNLSRQFLFRDADIHKPKSVAAAAAVRVMNSDVNIVAHEVRVGPETEATFDDEFWDGLSGVCNALDNVQARLYVDQRCVYYVKPLLDSGTLGTKGNTQVVVPGLTESYSSSRDPPEKSIPICTLKNFPYQIEHTLQWARDWFEGSFRGTPE---DCNAYLGS---RGKD-------FFDDLKKQ-GPGTMLATLEAVHSSLVTERPL-SVEDCIKWSRLLFEEMFSSSIKQLLHAFPADMVDANKVPFWSGTKRVPTPLTFNAADETHMAFVTSAALLRAQVYGIPVSDKDMAHFPAALAAVMVPDFVPKAGVKIATTEAEASEQNASG--AGSLDQDDERIETLLKALPPAGSAGSDGASKTLV---PLEFEKDDDTNHHIDVITACSNLRAANYSIEPADRHKSKMIAGKIIPAIATTTSFVTGLVCIEMLKLVRLGYTDRDGGGYDGGAWCKAAAKGSDAKARRDAAE-KKLECFKNGFANLALPFFAFSEPVVAAATDMG-HGRFWTLWDLLDVNEGRDITLGEFLEAIKTHYGLEVSMVSC-GVSILYASFTNPRRLQERLATPLSEVARTVGKLTFLA----------------KQRYLVMELICTDKEGEDVEVPYCRYRF 1081          

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|1065003537|gb|ODV92350.1| (hypothetical protein CANCADRAFT_56027 [Tortispora caseinolytica NRRL Y-17796])

HSP 1 Score: 692.575 bits (1786), Expect = 0.000e+0
Identity = 448/1152 (38.89%), Postives = 622/1152 (53.99%), Query Frame = 0

Query:   68 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDL-ETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGP-----LTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDV--EVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDET-LLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATLDEILASGYQAPTEVVRAEEEEEGDLE-AKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIECVDDA-GDEVESPPL 1208
            +DE+LYSRQLYVLG  A Q++ +SN+L++GLGG+GVEI KNV LAG +S+TL DPTPA  +DL+ QYYL    +G+ RA    +K+ ELN  V + +L+  ++D   V+         F V+V  +Q    +  +N   R   A F+++   GLFG  F D G+ FTV D  G+E  +G+L    P     + +VT  +  RH L  GD V F    TE  G            P L E D   +K ++ P  F+V    ++ K       G F Q+KQP  L F+PL  +      + +DF K    R   LH  F +L +F       LP P +  DA       K  D+  +V++ + + I+  +                +DE+++   A   +G+LAP+ + +GG AAQEVLKACTG F P +Q+L+FD++E L P   ED+              C   G +YDG  AVLG ++   + +  +F+VGAGAIGCE+LK  AL+GVG      +TVTD DSIE+SNLNRQFLFR  D+G  KSEAAA+AV  +NPD+  ++ A+  KVG +TE  FDD+FW   D+VANALDNV AR YVD RCV++R+PLLESGT GT GNVQVV+P +TESY SS DPPE SIP+CTLKSFP  IEH I WA+  F+ YF  Q E +N     LS  +             + +  + TSD+   L  L  YLV  +   P  S   C+ WA   F   + ++I  LL   P+DSVT  G PFWSG +R PTP+ FD ++P H  F+ +AA L+A  + +      VE   E  Q  +D ++   +   + +V  E++ + D E A    + ++++  I +I          +E    R  P  FEKD D N HI FIT+ASNLRA+NY +  ADR +TK IAG+IIPAIATTTA+V GL +LEL KL              +E + K+                +  ++N FINLA PFF  ++P+ +  I   ++ ++  +W R ++   KD+TL+  + H  +  GL IT V+ H   +LYA F  P       G  MTELV A  K E                +P     + FEI C DD  G++VE  P 
Sbjct:    8 IDESLYSRQLYVLGKEAMQQMKSSNVLIIGLGGLGVEIAKNVALAGVKSLTLYDPTPASFIDLSAQYYLTSADIGKPRAQACVSKVAELNEYVPVSVLD--TLDQSTVSA--------FKVVVCTNQSLELQLKVNEWTRASGAAFISSSVRGLFGVHFNDFGEKFTVLDPTGEEPVSGILSEISP-----DGVVTAHDETRHGLENGDYVRF----TEVQGM-----------PGLNEADPLPIK-VLGPYTFSV---GDVSKYGTYERGGVFHQVKQPKVLEFKPLVESIADPEYLVSDFAK--FDRPAQLHIGFIALDKF-IQQNSALPRPRNKEDAE------KLLDIAKNVVTEYPSIISDAN----------------IDEKLISELALQARGNLAPMTAMLGGFAAQEVLKACTGKFSPAKQWLYFDSLESLPPSLSEDD--------------CKEVGSRYDGNIAVLGKKVQDRISNLNMFLVGAGAIGCEMLKVWALLGVGAGPNGKITVTDMDSIERSNLNRQFLFRAPDVGKLKSEAAAKAVTNINPDLNGKIVAMQEKVGADTETIFDDDFWQPIDIVANALDNVDARTYVDRRCVFFRKPLLESGTLGTKGNVQVVIPDLTESYSSSRDPPEKSIPLCTLKSFPNQIEHTIAWAKSMFEEYFTNQEEMVN---MYLSNPNF------------IENTLSTTSDQIGTLNTLYNYLVAAK---PT-SFEDCIAWARLEFETKFHNDIVQLLYNFPKDSVTSSGIPFWSGPKRAPTPLTFDINNPTHFGFIQAAANLRAYNYGI-----KVEDSREVYQKVIDSLIIPDFVPKSGIVIKEKDTDPDPEGAAGMSDTDQIQELISKIPP-------ASEFGNFRLNPVEFEKDDDSNFHIDFITAASNLRASNYCIEPADRSKTKFIAGKIIPAIATTTALVTGLVNLELLKL--------------VEGKEKD----------------IEVFKNGFINLALPFFGFSEPIASGKIKYNDK-QIDQIWGRFDIY--KDLTLQEFIDHFEKEEGLSITMVS-HNVSLLYASFFPPKKLKEKYGMKMTELVSAVSKKE----------------IPAGTKTLLFEI-CADDKEGNDVEILPF 1004          

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|1183320252|gb|ORX50072.1| (ubiquitin-activating enzyme E1 [Piromyces finnis])

HSP 1 Score: 678.322 bits (1749), Expect = 0.000e+0
Identity = 428/1154 (37.09%), Postives = 620/1154 (53.73%), Query Frame = 0

Query:   68 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGP-----LTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVE--VRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQT-SDETLLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATLDEILASGYQAPTEVVRAEEEEEGDLEAKV--QREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIE-CVDDAGDE-VESPPLR 1209
            +DE+LYSRQLYVLG  A  R++ SN+L++GL G+GVEI KNVILAG +SVTL DP   ++ DL++Q++L E  +G+ R   S  +LKELN  V + +L+   M          +S  N+ V+V  +     +  +N +   L  KF++    GLFG  F D GD F V D  G+E K+G++ + +      +++VT  +  RH+L  GD V F    TE  G T+        +P   +       ++ P  F++  ++NL   V   S G F Q+KQP  L F+ L  + +      +DF K    R   LH  F++L  F     GR P+P +  DA          DVL         AIA             +P+P  +D+++++  A   +G L+P+ +  GG  AQEVLKA +G F+P+ QY++FD++E L              + LP+E  CA    +YD   AV G    + +++   F+VGAGAIGCE+LKN A+MG+G      + +TD DSIEKSNLNRQFLFR  D+   KSE AA A+ ++N   E  ++A   +VG ETE  F+D+FW     V NALDNV ARKYVD RCV++R+PL+ESGT GT GN QV++P +TESY SS DPPE +IP+CTLK+FP  IEH IQWARD F+  F+   + +N     L++ ++            + S+R Q  + +  ++ +  YLV    W P  + R C+ WA + F ELY ++I+ LL   P+DSVT  G+PFWSG +R PTPI+FD ++ LHMDF+++AA L A  + +   T      +E    T+ +        P + V+ + +E  +++  +  +RE EE+   IK+I          +     R  P  FEKD D N HI FIT+ASNLRA NY +  ADR +TK IAG+IIPAIATTTA+V GL  LEL K+              ++ +NK                 L  ++N F+NLA PFF  ++P+ A  +   +    + +WDR +V  + D+TL+ L+    E   L+IT ++  G  +LY+ FM P      L   ++ LVE   K                   P+  H     +E CV+D  DE VE P +R
Sbjct:    7 IDESLYSRQLYVLGREAMLRMSQSNVLIIGLRGLGVEIAKNVILAGVKSVTLYDPELTKLSDLSSQFFLTEEDVGKRRDEVSCPRLKELNNYVPVTVLDGDLM----------SSITNYQVVVCTNTPLKQQIEINNITHPLGIKFISTSTNGLFGMAFNDFGDAFEVTDQTGEEPKSGMVAAINK--SADQSVVTCLDETRHNLEDGDYVTF----TEVKGMTE----LNGCEPRKIS-------VLGPYTFSIGDTSNLSDYV---SGGLFHQVKQPKTLKFKSLVDSIKEPELFISDFAK--FDRPGQLHVGFQALDSF-VEKNGRYPVPRNENDAN---------DVL---------AIAKEING-------NSPNPVEIDDKLIKELAYQSRGDLSPMAALFGGLVAQEVLKAISGKFMPINQYMYFDSLESL-------------PSQLPTEASCAPINSRYDNQIAVFGKEFQEKIENTKEFLVGAGAIGCEMLKNWAMMGLGVGKDGYIYLTDMDSIEKSNLNRQFLFRPWDVTKLKSETAAAAIHKMNKQTEGHIKAFSDRVGTETENIFNDDFWESLSSVTNALDNVDARKYVDLRCVFFRKPLIESGTLGTKGNTQVIIPHLTESYSSSQDPPEKAIPICTLKNFPNAIEHTIQWARDKFEELFKQSADNVN---LHLTQPNY------------IDSVRKQAGTQKDTIQGIYNYLV---AWKP-KTFRECIIWAREMFEELYNNSIQQLLFNFPKDSVTSSGQPFWSGPKRAPTPIIFDMNNQLHMDFIIAAANLHAFNYGLTGETDVQVFKNELATITVKKF------EPKKGVKIQVQENENVQNALPDEREIEEL---IKKIP-------SPSTLVGYRMNPADFEKDDDTNFHIDFITAASNLRATNYEIGLADRSKTKFIAGKIIPAIATTTALVTGLDCLELYKI--------------IDGKNK-----------------LEDFKNGFLNLALPFFGFSEPIAAQKMKYYDIE--WTLWDRFDV--EGDITLQELIDLFKEKHELEITMMS-SGVSMLYSSFMQPKKLQERLNMKISTLVETVSKK------------------PIPDHIKSLVLELCVNDKNDEDVEVPYVR 1000          

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|939242362|ref|XP_014240445.1| (PREDICTED: ubiquitin-like modifier-activating enzyme 1 [Cimex lectularius])

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 441/1165 (37.85%), Postives = 622/1165 (53.39%), Query Frame = 0

Query:   68 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGP----LTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDET-LLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVF---NVVDPTTTVEGMSETVQ-----------ATLDEILASGYQAPTEVVRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIECVDDAGDEVESPPLRYFFP 1213
            +DE LYSRQLYVLG  A +R+A+S++L+ GLGG+GVE+ KNVIL G +SVTL D     + DL++Q+YL E  +G++RA  S  +L ELN  V      P+      + E      R F V+V AD DK  +E    LAR      VAA + G+F  +FCD G +FTV DT+G+   + ++ +   +    E +V   +  RH L  GD V F      ++    + ++                +++ P  F++  + +  + +     G  +Q+K P ++ F+P+  + ++   V TDFGK    +QL  H  F +L+ F     G  P P    DA+AF +L   C   +DV+               P ++        L+E ++  FA TC GSL P+ +F+GG  AQEV+KAC+G F P+ Q+L+FDA+E L     E  +PE          +C  +G++YDG  AV G+   K+L     FVVGAGAIGCELLKN A+MGVG     +TVTD D IEKSNLNRQFLFR  D+  PKS  A++A++++NP + V     +VG ETE+ +DD F+   D VANALDNV AR Y+D RCVYYR+PLLESGT GT GN QVVVPF+TESY SS DPPE SIP+CTLK+FP  IEH +QWARD F+G F+   E   +    LS+ +            +  +LR Q      ++  L+  L+D    D     R CV WA + + E Y + I  LL   P D VT  G+PFWSG +R P P+VFD +DPLH+D++ +AA LKA V+    V D     E + E V            A  D  L +G   P      ++++ G L+A+              +  R   NG        + RP  FEKD D N H+ FI +ASNLRAANYG+  ADR ++K IAG+IIPAIATTT+VVAGL   EL KLAQ               + K                 L  ++N F+NL+ PFF  ++P+ A      +    + +WDR EV  + +MTL+  + +  +  GL+IT ++  G  +LY+ FM           +M+E+V        R + ++ +E       P  +  V FE+ C D+ G++VE P +RY  P
Sbjct:   50 IDEGLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEVAKNVILGGVKSVTLHDDKKCTLADLSSQFYLTEADVGKNRAEASCRQLAELNNYV------PTRAYTGPLTEDFL---RGFRVLVLADVDKDVKERTAELARTSGVALVAATSRGVFAQVFCDFGPEFTVLDTNGENPVSAMIAN---ISRDAEGVVACLDDSRHGLEDGDYVTFHEVQGMKELNECEPMKI---------------KVLGPYTFSIGCTTDFSEYLR---GGIVQQVKMPKKVVFKPMKESVKSPEFVMTDFGKFDHPQQL--HIAFHALEMF-IKRNGLPPKPWDQKDASAFLEL---CKTQTDVL---------------PGTE--------LNEDLIRKFAYTCSGSLNPMNAFIGGVVAQEVMKACSGKFSPIFQWLYFDAIECLP----EKLAPE----------DCVLKGNRYDGQVAVFGNSFQKVLGSLKYFVVGAGAIGCELLKNFAMMGVGADGGQITVTDMDLIEKSNLNRQFLFRPHDVQKPKSGTASRAIKKMNPHMCVVPHENRVGPETEKVYDDVFFENLDGVANALDNVDARIYMDRRCVYYRKPLLESGTLGTKGNTQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDSFEGLFKQSAE---NAALYLSDPTF-----------IERTLRLQGVQPVEVMESLKQALIDERPED----FRDCVVWARNHWQEQYSNQIRQLLFNFPPDQVTSSGQPFWSGPKRCPKPLVFDVNDPLHLDYVFAAANLKAEVYGLRQVRDRDAVAEMVKEVVVPEFTPKSGVRIAINDAQLTNGAGGPI-----DQDKIGQLQAE--------------LPSRESFNG-------MQIRPLEFEKDDDNNLHMDFIVAASNLRAANYGIPPADRHKSKLIAGKIIPAIATTTSVVAGLVCFELIKLAQ--------------GEKK-----------------LEKFKNGFVNLSLPFFGFSEPIAAPKQKYYDHE--WTLWDRFEV--QGEMTLQEFIDYFKKEHGLEITMLS-QGVCMLYSFFMPSSKLQDRHNLTMSEVV--------RKVSKRKLE-------PHVKALV-FELCCNDNDGNDVEVPYVRYTLP 1045          

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|1183360789|gb|ORX88138.1| (ubiquitin-activating enzyme E1 [Anaeromyces robustus])

HSP 1 Score: 675.241 bits (1741), Expect = 0.000e+0
Identity = 426/1152 (36.98%), Postives = 613/1152 (53.21%), Query Frame = 0

Query:   68 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGP-----LTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVE--VRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDET-LLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATLDEILASGYQAPTEVVRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIE-CVDDAGDE-VESPPLR 1209
            +DE+LYSRQLYVLG  A  R++ SN+L++GL G+GVEI KNVILAG +SVTL DP   ++ DL++Q++L E  +G+ R   S  +LKELN  V + +L    M          +S  N+ V+V  +     +  +N +      KF++ +  GLFG  F D GD F V D +G+E K+G++ +        +++VT  +  RH+L  GD V F    TE  G T+        +P   T       ++ P  F++  ++NL   V   S G F Q+KQP  L F+ L  + +      +DF K    R   LH  F++L  F     GR P+P +  DA     L K  +                         + +P+P  LD+ +++  A   +G L+P+ +  GG  AQEVLKA +G F+P+ QY++FD++E L              + LP+E +CA  G +YD   AV G    + +++   F+VGAGAIGCE+LKN A+MG+G      + +TD DSIEKSNLNRQFLFR  D+   KSE AA A+ ++N D E  ++A   +VG ETE  F+D FW     V NALDNV ARKYVD RCV++R+PL+ESGT GT GN QV++P +TESY SS DPPE +IP+CTLK+FP  IEH IQWARD F+  F+   + +N     L++ ++            L S+R Q  ++   ++ +  YLV    W P  + R C+ WA + F ELY ++I+ LL   P+DSVT  G+PFWSG +R PTPI FD ++ LHMDF+++AA L A  + +   T      +E    T+ +        P + V+ +  +  +++  +  E +E+   IK+I          +     R  P  FEKD D N HI FIT+ASNLRA NY +A ADR +TK IAG+IIPAIATTTA+V GL  LEL K+              ++ +NK                 L  Y+N F+NLA PFF  ++P+ A  +   +    + +WDR +V    D+TLK L+    E   L+IT ++  G  +LY+ F+        L   ++ LVE   K                   P+  H     +E CV+D  DE VE P +R
Sbjct:   10 IDESLYSRQLYVLGREAMLRMSQSNVLIIGLRGLGVEIAKNVILAGVKSVTLYDPEITQLSDLSSQFFLTEEDVGKRRDEASCPRLKELNNYVPVSVLNDDLM----------SSITNYQVVVCVNTPLKQQIEINNITHPNGIKFISTRTNGLFGMSFNDFGDTFEVTDQNGEEPKSGMVAAI--TKSAEQSVVTCLDETRHNLEDGDYVTF----TEVKGMTE----LNGCEPRKIT-------VLGPYTFSIGDTSNLSDYV---SGGLFHQVKQPKTLSFKSLVDSLKEPEMFISDFAK--FDRPGQLHVGFQALDSF-VEKNGRYPVPRNENDANDVLALAKEIN-------------------------SSSPNPVELDDNLIKELAFQSRGDLSPMAALFGGLVAQEVLKAISGKFMPITQYMYFDSLESL-------------PSQLPNEADCAPIGSRYDNQIAVFGKEFQEKIENTKEFLVGAGAIGCEMLKNWAMMGLGIGKDGFIYLTDMDSIEKSNLNRQFLFRPWDVTKLKSETAANAIHKMNKDTEGHIKAFSDRVGTETENIFNDEFWESLSGVTNALDNVDARKYVDLRCVFFRKPLIESGTLGTKGNTQVIIPHLTESYSSSQDPPEKAIPICTLKNFPNAIEHTIQWARDKFEELFKQSADNVN---LHLTQPNY------------LESIRKQAGNQKDTIQCIYNYLV---AWKP-KTFRECIIWAREIFEELYNNSIQQLLFNFPKDSVTSSGQPFWSGPKRAPTPITFDMNNQLHMDFIIAAANLHAFNYGLTGETDVQVFKNELATITVKKF------EPKKGVKIQVNDNENVQNVLPDE-KEIDELIKKIP-------SPSTLVGYRMNPADFEKDDDTNFHIDFITAASNLRATNYEIALADRSKTKFIAGKIIPAIATTTALVTGLDCLELYKV--------------IDGKNK-----------------LEDYKNGFLNLALPFFGFSEPIAAQKMKYYDIE--WTLWDRFDVV--GDITLKELIELFKEKHELEITMMS-SGVSMLYSSFLPQKKLQERLNMKISTLVETISKK------------------PIPDHVKSLVLELCVNDKNDEDVEVPYVR 1003          

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|909130504|gb|KNE57376.1| (ubiquitin-activating enzyme E1 [Allomyces macrogynus ATCC 38327])

HSP 1 Score: 671.389 bits (1731), Expect = 0.000e+0
Identity = 440/1151 (38.23%), Postives = 605/1151 (52.56%), Query Frame = 0

Query:   68 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGP-----LTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDETLLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMS--ETVQATLDEILASGYQAPTEV---VRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIECVDDAGDEVESPPL 1208
            +DE LYSRQ+Y +GL A +++A SN+L++G+ G+GVEI KNV+LAG +SVT+ DP P  + DL++Q++L E  LG+ R   +  KL ELN  V + +LE  ++D+  +         +F V+VA+         LN        KF+AA   GL    FCD GD+F   DT G+   +G++ +   +    E +VT  +  RH L  GD V F      +       V  G     +        +++ P  FT+  + +L   V     G F Q+K P  L F+PL+ +      + +DF K  +  QL  HA F +L  F T  G R P P +  +A    +L K                   +  K P           ++E++++ FA    G L+P+ + +GG  AQEVLKAC+G F P++QYL+FD+ E L         P  ++ +   E  CA RG +YDG  AV G+   + + D   FVVGAGAIGCE+LKN ALMG+G      + VTD DSIEKSNLNRQFLFR  D+   KSE AA+AV+ +NP V++     +VG ETE  F+D FW   D V NALDNV AR+YVDGRCV+YR+PLLESGT GT GN QV++P VTESY SS DPPE SIP+CTLK+FP  IEH IQWARD F+G FR   E +N     LS  +            V  +LR  T+ ETLL  ++ YLVD     P  S   CV WA  +F EL+ +NI+ LL   P+DSVT  G+ FWSG +R P  +VFD ++ LH+DF++ AA L A  FN       ++G    E  +  L  I    +     V   V+  E+   D     Q E + +   + + A+  G           R  P  FEKD D N HI FI + SNLRA+NY +A ADR +TK IAG+IIPAIATTTA+V GL  LEL KL              L++Q K                PL  ++N F+NLA PFF  ++P+ A  +        + +WDR EV    +MTL+ LL H      L+IT ++  G  +LY+ FM        L  S+++LVE   K                + +P     +  E+ C D  G++VE P +
Sbjct:   17 IDEGLYSRQIYAIGLDAMKKMALSNVLIIGMKGLGVEIAKNVVLAGVKSVTIHDPEPVEIADLSSQFFLTEKDLGKPRDQVTCPKLAELNQYVPVTVLE-GALDLPRL--------DDFQVVVASSLPMHRCIELNDYTHARGIKFIAADTRGLAALAFCDFGDEFECVDTTGEAPLSGMIAA---VSNDAEGVVTCLDETRHGLEDGDYVTFAEVVGMD-------VLNGCEPRKV--------KVLGPYTFTIGDTTSLGTYVR---GGIFTQVKMPQTLKFKPLSASIADPEFLMSDFAKFDVPPQL--HAAFMALSTFLTKKG-RAPAPRNDQEADTIVQLAKEF----------------AATMKDPVE---------INEKLIKQFAYQAAGDLSPMAAVIGGMVAQEVLKACSGKFHPIKQYLYFDSFESL---------PTGYALT---EAACAPRGTRYDGQIAVFGAEYQQRMHDTREFVVGAGAIGCEMLKNYALMGLGTSDNGHIFVTDMDSIEKSNLNRQFLFRPWDVSKLKSETAARAVKNMNPAVQITTFTDRVGVETESVFNDEFWMALDGVTNALDNVDARRYVDGRCVFYRKPLLESGTLGTKGNTQVILPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTIQWARDSFEGLFRQPAENVN---LYLSTPNF-----------VDTALRGNTARETLL-SIKEYLVDAR---PT-SFDECVAWARHKFEELFVNNIKQLLHNFPKDSVTSTGQLFWSGPKRAPDALVFDATNDLHLDFVVYAAKLHA--FNY-----GLKGQDDREYYRKVLKTIHVPAFVPRDGVKIQVQENEQPNNDSATADQDELDTLVAALPKPAELAG----------FRLTPVDFEKDDDSNHHIDFIAACSNLRASNYNIAIADRHKTKFIAGKIIPAIATTTALVTGLVCLELYKL--------------LDAQPK----------------PLDQFKNGFVNLALPFFGFSEPIAAPKMKYNNVE--FTLWDRFEV---GNMTLRELLKHFETQHQLEITMMSC-GVSMLYSFFMPKKKVEERLNMSLSQLVETVAK----------------KPVPPHAKTLVLEVCCNDVEGEDVEVPSI 1009          

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|1183512884|gb|ORZ36115.1| (hypothetical protein BCR44DRAFT_1479631 [Catenaria anguillulae PL171])

HSP 1 Score: 667.922 bits (1722), Expect = 0.000e+0
Identity = 434/1145 (37.90%), Postives = 610/1145 (53.28%), Query Frame = 0

Query:   68 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGP-----LTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDETLLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNV-VDPTTTVEGMSETVQATLDEILASGYQAPTEVVRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIECVDDAGDEVESP 1206
            +DE LYSRQ+Y +GL A +++A SN+L++GL GVGVE+ KNVILAG +SVTL DP PA+  DL++Q+YL E  +GQ R + S AKL ELN  V + +L+    D+  +A+        F V+V A         +N        KF+A    GL    FCD G DF + DT+G++   G++ +       G  +VT  +  RH L  GD V F    TE  G  K        +P          +++ P  F++  ++ L   V     G F+Q+K P+++ F+ L  +      + +DF K     QL  HA F +L  F T   GR+P P +  DA A              + + + A AA S  K P           ++E++++ FA    G L+P+ + +GG  AQEVLKAC+G F PL+QYL+FD+ E L         P+++S S   + +CA RG +YDG  AV GS   K +Q+   FVVGAGAIGCE+LKN A+MG+G      + VTD DSIEKSNLNRQFLFR  D+   KS+ AA AV+ +NP++++     +VG+ETE  F+D+FW   DVV NALDNV ARKYVD RCV++ +PLLESGT GT GN QVV+P  TESY SS DPPE SIP+CTLK+FP  IEH IQWARD F+G FR   E +N    +              A  V  +LR  ++ ETL+   E YLVD        +   C++WA  +F E Y ++I+ LL   P+DSVT  G+ FWSG +R P  + FD+++PLHMDF++ AA L A  FN  + PTT     ++ + A +            +V   E +      +    + +E+   +  +          +     R  P  FEKD D N HI FIT+ SNLRA NY +A ADR QTK IAG+IIPAIATTTA++ GL  LEL KL                  NK                P+  ++N F+NLA PFF  ++P+ A  +   ++   + +WDR EV    ++TL  +L   +    L IT ++  G  +LY+ FM          K + E    AMK      ++  V   + + +P     +  E+   D++G++VE P
Sbjct:   15 IDEGLYSRQIYAIGLDAMRKMALSNVLILGLKGVGVELAKNVILAGVKSVTLYDPEPAQPADLSSQFYLTEADIGQRRDLASVAKLAELNQYVPVTVLDGDLSDLARLAQ--------FQVVVCAGLPLHKCIEINNYTHANGIKFLAVDTRGLAALGFCDFGADFEIIDTNGEQPVQGMIAAISADAKDG--VVTCLDETRHGLEDGDFVTF----TEIQGMDK----LNDCEP-------RKVKVLGPYTFSIGDTSGLGTYVR---GGIFKQVKLPVRMSFKSLAASLADPEFLVSDFAKFDHPAQL--HAAFYALSEFITENNGRVPTPRNEDDANA--------------VLAKAKAFAA-STLKDPID---------INEKLIKQFAYQAAGDLSPMAAVMGGFMAQEVLKACSGKFSPLKQYLYFDSFESL---------PQSYSLS---QSKCAMRGTRYDGQIAVFGSDFQKRVQETRNFVVGAGAIGCEMLKNYAMMGLGTQGDGHVFVTDMDSIEKSNLNRQFLFRPWDVSKLKSDTAAGAVRVMNPELQITTFTDRVGQETEHVFNDSFWQSLDVVTNALDNVEARKYVDRRCVFFCKPLLESGTLGTKGNTQVVLPHKTESYSSSQDPPEKSIPICTLKNFPNQIEHTIQWARDLFEGLFRQPAENVNLYLTQ--------------ANFVEQTLRGNSARETLVSVKE-YLVDAR----PKTYEACIQWARLKFEEYYVNSIKQLLHNFPKDSVTSTGQLFWSGPKRAPDVVAFDRTNPLHMDFVVHAAHLYA--FNYGLKPTTDASVYAKVLDAMVVPEFVPKDGVKIQVNEQEAQAAAANASGSATDGDEIDQLVASLPPP-------STLAGFRLNPCEFEKDDDSNHHIDFITACSNLRATNYNIAVADRTQTKFIAGKIIPAIATTTALITGLVCLELYKLL---------------DNNK----------------PIEQFKNGFVNLALPFFGFSEPIAAPKLKYNDKE--FTLWDRFEV---PNVTLGEILDLFATKHKLNITMMS-SGVSMLYSFFMPK--------KKVDE--RKAMK------IKDVVASVSKKEIPKHAKSLVLEVCAEDESGEDVEVP 1012          

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|1183438564|gb|ORY62751.1| (ubiquitin-activating enzyme E1 [Neocallimastix californiae])

HSP 1 Score: 667.922 bits (1722), Expect = 0.000e+0
Identity = 428/1158 (36.96%), Postives = 618/1158 (53.37%), Query Frame = 0

Query:   68 VDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRI--VSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPS----EKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVGP-----LTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDV--EVRALVRKVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQT-SDETLLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATLDEILASGYQAPTEVVRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIE-CVDDAGDE-VESPPLR 1209
            +DE+LYSRQLYVLG  A  R++ SN+L++GL G+GVEI KNVILAG +SVTL DP   ++ DL++Q++L E  +G+ R   S  +LKELN  V + +L+   M          ++  N+ V+V  +     +  +N +      KF++ +  GLFG  F D GD F V D  G+E K+G++ +        +++VT  +  RH+L  GD V F    TE  G T+        +P         K+I  + P  F++  ++NL   V   S G F Q+KQP  L F+ L  + +      +DF K    R   LH  F++L  F     GR PIP +  DA          DVL+     +SA+                P+P  +D+++++  A   +G L+P+ +  GG  AQEVLKA +G F+P+ QY++FD++E                 SLPS    E+ CA  G +YD   AV G    + +++   F+VGAGAIGCE+LKN A+MG+G      + +TD DSIEKSNLNRQFLFR  D+   KSE AA A+ ++N      +++   +VG ETE  F+D+FW     V NALDNV ARKYVD RCV+YR+PL+ESGT GT GN QV++P +TESY SS DPPE SIP+CTLK+FP  IEH IQWARD F+  F+   + +N     L++ ++            L S+R Q  + +  ++ +  YLV    W P  + R C+ WA + F ELY ++I+ LL   P+DSVT  G+PFWSG +R PTPIVFD ++ LHMDF+++AA L A  + +   T      +E    T+ +        P + V+ +  E  +++  +  E +E+   I +I          +     R  P  FEKD D N HI FIT+ASNLRA NY +A ADR +TK IAG+IIPAIATTTA+V GL  LEL K+              ++ +NK                 L  Y+N F+NLA PFF  ++P+ A  +   +    + +WDR ++  + D+TL+ ++    E + L+IT ++  G  +LY+ FM        L   ++ LVE   K                   P+  H     +E CV+D  DE VE P +R
Sbjct:   10 IDESLYSRQLYVLGREAMLRMSQSNVLIIGLRGLGVEIAKNVILAGVKSVTLYDPELTQLSDLSSQFFLTEEDVGKRRDEASCPRLKELNNYVPVSVLDGDLM----------SNITNYQVVVCTNVPLKQQIEINNITHPNGIKFISTRTNGLFGMTFNDFGDAFEVTDQTGEEPKSGMVAAI--TKSADQSVVTCLDETRHNLEDGDYVTF----TEIKGMTE----LNGCEP---------KKISVLGPYTFSIGDTSNLSDYV---SGGLFHQVKQPKTLKFKNLVDSIKEPEMFISDFAK--FDRPGQLHVGFQALDSF-VEKNGRYPIPRNENDAN---------DVLAIAKEINSAS----------------PNPVDIDDKLIKELAYQSRGDLSPMAALFGGLVAQEVLKAISGKFMPISQYMYFDSLE-----------------SLPSQPLTEELCAPIGSRYDNQIAVFGKDFQEKIENTKEFLVGAGAIGCEMLKNWAMMGLGVGKNGFIYLTDMDSIEKSNLNRQFLFRPWDVTKLKSETAANAIHKMNKQTIGHIKSYSDRVGVETENIFNDDFWESLSGVTNALDNVDARKYVDLRCVFYRKPLIESGTLGTKGNTQVIIPHLTESYSSSQDPPEKSIPICTLKNFPNAIEHTIQWARDKFEELFKQSADNVN---LHLTQPNY------------LDSVRKQAGTQKDTIQSIYNYLV---TWKP-KTFRECIIWAREIFEELYNNSIQQLLFNFPKDSVTSSGQPFWSGPKRAPTPIVFDMNNKLHMDFIIAAANLHAFNYGLTGETDVQVFKNELATITVKKF------EPKKGVKIQVNENENVQNALPDE-KEIEDLIAKIP-------SPSTLVGYRMNPAEFEKDDDTNFHIDFITAASNLRATNYDIALADRSKTKFIAGKIIPAIATTTALVTGLDCLELYKI--------------IDGKNK-----------------LEDYKNGFLNLALPFFGFSEPIAAQKMKYYDVE--WTLWDRFDI--EGDLTLQQIIDLFKEKYELEITMMSC-GVSMLYSSFMQSKKLQERLNMKISTLVETVSKK------------------PIPDHVKSLVLELCVNDKNDEDVEVPYVR 1003          

BLAST of EWM30334.1 vs. NCBI_GenBank
Match: gi|1183466416|gb|ORY90353.1| (ubiquitin-activating emzyme E1 [Syncephalastrum racemosum])

HSP 1 Score: 663.3 bits (1710), Expect = 0.000e+0
Identity = 427/1161 (36.78%), Postives = 620/1161 (53.40%), Query Frame = 0

Query:   57 LEQQRIQASIVVDEALYSRQLYVLGLPAQQRLAASNILVVGLGGVGVEITKNVILAGPQSVTLIDPTPARMVDLATQYYLDETSLGQSRAMCSYAKLKELNPLVQLDLLEPSSMDIEAVAEAIAASGRNFDVIVAADQDKASEEALNTLARRLKAKFVAAQAPGLFGSIFCDMGDDFTVNDTDGKEAKTGLLKSFDPLPMTGETLVTLREGERHDLSKGDLVYFERTATEEDGQTKDAVRAGSADPDLETDTFTVKRIVSPSCFTVPASANLVKQVHQYSNGRFRQLKQPLQLGFQPLTLARQAMRSVPTDFGKASLGRQLTLHACFESLKRFKTHAGGRLPIPGSATDAAAFKKLVKSCDVLSDVISSHSAAIAARSAAKRPTSKTRAPSPPVLDEQIMEIFARTCQGSLAPICSFVGGTAAQEVLKACTGTFVPLQQYLFFDAVEVLTPESGEDESPEAFSASLPSEKECASRGDKYDGMRAVLGSRLMKLLQDQAIFVVGAGAIGCELLKNLALMGVG-----PLTVTDNDSIEKSNLNRQFLFRERDLGAPKSEAAAQAVQRLNPDVEVRALVR--KVGKETEEFFDDNFWFGHDVVANALDNVAARKYVDGRCVYYRRPLLESGTEGTSGNVQVVVPFVTESYGSSVDPPEASIPVCTLKSFPYMIEHCIQWARDFFDGYFRIQPERLNDLTARLSESSHRGEEDDAAAAAVLASLRAQTSDETLLRELEGYLVDLEIWDPVPSARRCVKWALDRFHELYQSNIEALLREHPRDSVTEDGEPFWSGDRRIPTPIVFDQSDPLHMDFLLSAALLKARVFNVVDPTTTVEGMSETVQATLDEILASGYQAPTEVVRAEEEEEGDLEAKVQREFEEVRLTIKRIAKRGGDNGKGTECTAARTRPEMFEKDQDWNGHIAFITSASNLRAANYGLARADRLQTKKIAGRIIPAIATTTAVVAGLASLELCKLAQRSLLEEEEEYSDLESQNKEEGLLKQRRRHRRHYPPLSAYRNAFINLAGPFFALTQPMEAHPIALGEQGEVYNMWDRLEVTPKKDMTLKSLLAHASENFGLQITSVTLHGSHILYADFMHPGDDNPILGKSMTELVEAAMKSENRAIVRKGVEGTNIQGMPLRRHFVDFEIECVDDAGDE-VESPPLR 1209
            + +  + A   +DE LYSRQLYVLG  A ++++ +++LV+GL G+GVEI KNVILAG +SVTL DPTPA + DL+ Q+Y+ E  +GQ RA  S  +L ELN  V + +L+          E   A+ + F  +VA +   + +  ++ +       F++ +  GLFG IF D G +F + D  G+E  TG++ +   +    E +VT  +  RH L  GD V F    TE  G T           +L   T    +++ P  F++  ++NL   V     G F Q+K P  + F+    +      + +DF K    R + LH  F++L +F      R P P +  DA   +++ ++   L D +                         P LDE+ ++  A    G ++P+ +  GG AAQEVLK+ +G F P+ QY+F DA+E L             S  L SE+ CA  G +YDG  AV G    + +++   F+VGAGAIGCE+LKN A+MG+G      + +TD D+IEKSNLNRQFLFR  D+G  KSE AA AV  +NP ++ +  V   +VG +TE  +DD+F+     V NALDNV ARKYVD RCVYYR+PLLESGT GT GN QVV+P++TESY SS DPPE +IP+CTLK+FP  IEH IQWARD F+GYF+   + +N     L++ +            V  +++   S +  L+ +  YLV      P+ S   CV WA  +F E+Y +NI+ LL   P+DSVT  G+PFWSG +R P PI FD +DP+HMDF++ AA L A  + +   T       E  +  L+ ++   ++ P E V+ + +E   ++     +      ++  + K   D    +     R  P  FEKD D N HI FIT+ASNLRA NYG+A ADR +TK IAG+IIPAIATTTA+V GL  LEL K+              ++ +N                  L  Y+N F+NLA PFF  ++P+ A    L   G+ + +WDR ++  K+D+TL+  + +      L++T V+  G  +LY+ FM        L   +++LVE+  K                + +P     + FE+ CV+DA DE VE P +R
Sbjct:    1 MSRMNVDAENTIDEGLYSRQLYVLGHEAMKKMSVAHVLVIGLRGLGVEIAKNVILAGVKSVTLYDPTPATISDLSAQFYIREDEVGQPRAQVSQPRLAELNQYVPVKVLQ---------GELDEATLKQFKCVVATELPFSKQLEVSEICHANNIHFISTEVRGLFGRIFNDFGKNFEIIDATGEEPLTGMVAA---ITKDKEGVVTCLDETRHGLEDGDAVTF----TEVQGMT-----------ELNGITPRKIKVLGPYTFSIGDTSNLGDYVR---GGIFNQVKLPKYVDFKSYKESLSKPEFLISDFAK--FDRPMQLHLAFQALGQF-VEKHQRFPKPRNEQDA---EEVFQATKALVDTVEEK----------------------PELDEKFIKELAYQSAGEISPMVAVYGGLAAQEVLKSVSGKFHPIHQYMFLDALEALPT-----------SVKL-SEELCAPVGSRYDGQIAVFGREFQEKIENTKEFLVGAGAIGCEMLKNWAMMGLGAGPNGSIIITDMDTIEKSNLNRQFLFRPTDVGKLKSECAAAAVATMNPKLQGKISVHQERVGPDTENIYDDDFFESLSGVTNALDNVEARKYVDRRCVYYRKPLLESGTLGTKGNTQVVIPYLTESYSSSQDPPEKAIPICTLKNFPNAIEHTIQWARDLFEGYFKQSTDNVN---LYLTQPNF-----------VETTMKQGGSQKETLQTVYNYLV---AEKPL-SFSACVAWARIKFEEMYNNNIQQLLFNFPKDSVTSSGQPFWSGPKRAPEPIQFDPNDPIHMDFVVYAANLHAFNYGLKGETD-----REYFRKELENVIVPEFR-PKEGVKIQVQENETVDDNGGED------SLDDVIKNLPD---PSTLAGYRLNPCDFEKDDDSNFHIDFITAASNLRAINYGIAPADRHKTKFIAGKIIPAIATTTALVTGLVCLELYKI--------------IDGKNT-----------------LEDYKNGFVNLALPFFGFSEPIGAP--KLEYNGKQFTLWDRFDI--KQDITLQEFIDYFQNEEKLEVTMVS-SGVSMLYSFFMQKKKKEERLAMKLSKLVESVSK----------------KPIPPHVKALIFEV-CVNDADDEDVEVPYVR 1005