EWM26327.1, cds4355 (CDS) Nannochloropsis gaditana

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|585108334|gb|EWM26327.1| (multidrug resistance-associated protein 4 [Nannochloropsis gaditana])

HSP 1 Score: 3042.29 bits (7886), Expect = 0.000e+0
Identity = 1493/1493 (100.00%), Postives = 1493/1493 (100.00%), Query Frame = 0

Query:    1 MLLRMGSDRFNHKLYLHSLCHPTAMPQAEADAALTPPPAPPPPPSPPVDLYERLTPWQLLTFTFVFPIVRYGTYKPLQQVDLLPVAKRDRAARLLTCLEGEWDMEMRAYKYPSLNRALYRTHRRVFYLCGALLFTEYAIMLTKPVVLRYLLAWLKGTTYIEADVGVYLHWGYGFGVALALAGLDFMQTLVHHRAFYLTIRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAITLGLFFVAPSLAAFVTFLTYWALGNHLELPTVFAVMTLLQTLRECARWVPRSIEQVSEALVASRRILPLLLMAEAGPSTQNCTEAGSSGGKKEGGGAKGRKAQWGRRPSATRAFAEVSSNKSSLLLGSRDPQGKELGARLLPPPPALPPSIPPLPTLTAPPTQPSVGNRGDILVEIEDSGFSWATGNVKGQGIDWQARAEFQLSLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYGSGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKAEEAEEGEGMPKDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLISQGVLTFADFWLKMWASDQDHARTGAYYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSLDLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRETRGVDTADTAMSKAENLG 1493
            MLLRMGSDRFNHKLYLHSLCHPTAMPQAEADAALTPPPAPPPPPSPPVDLYERLTPWQLLTFTFVFPIVRYGTYKPLQQVDLLPVAKRDRAARLLTCLEGEWDMEMRAYKYPSLNRALYRTHRRVFYLCGALLFTEYAIMLTKPVVLRYLLAWLKGTTYIEADVGVYLHWGYGFGVALALAGLDFMQTLVHHRAFYLTIRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAITLGLFFVAPSLAAFVTFLTYWALGNHLELPTVFAVMTLLQTLRECARWVPRSIEQVSEALVASRRILPLLLMAEAGPSTQNCTEAGSSGGKKEGGGAKGRKAQWGRRPSATRAFAEVSSNKSSLLLGSRDPQGKELGARLLPPPPALPPSIPPLPTLTAPPTQPSVGNRGDILVEIEDSGFSWATGNVKGQGIDWQARAEFQLSLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYGSGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKAEEAEEGEGMPKDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLISQGVLTFADFWLKMWASDQDHARTGAYYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSLDLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRETRGVDTADTAMSKAENLG
Sbjct:    1 MLLRMGSDRFNHKLYLHSLCHPTAMPQAEADAALTPPPAPPPPPSPPVDLYERLTPWQLLTFTFVFPIVRYGTYKPLQQVDLLPVAKRDRAARLLTCLEGEWDMEMRAYKYPSLNRALYRTHRRVFYLCGALLFTEYAIMLTKPVVLRYLLAWLKGTTYIEADVGVYLHWGYGFGVALALAGLDFMQTLVHHRAFYLTIRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAITLGLFFVAPSLAAFVTFLTYWALGNHLELPTVFAVMTLLQTLRECARWVPRSIEQVSEALVASRRILPLLLMAEAGPSTQNCTEAGSSGGKKEGGGAKGRKAQWGRRPSATRAFAEVSSNKSSLLLGSRDPQGKELGARLLPPPPALPPSIPPLPTLTAPPTQPSVGNRGDILVEIEDSGFSWATGNVKGQGIDWQARAEFQLSLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYGSGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKAEEAEEGEGMPKDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLISQGVLTFADFWLKMWASDQDHARTGAYYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSLDLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRETRGVDTADTAMSKAENLG 1493          

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|1001605637|gb|KXS09504.1| (hypothetical protein M427DRAFT_128884 [Gonapodya prolifera JEL478])

HSP 1 Score: 722.235 bits (1863), Expect = 0.000e+0
Identity = 492/1442 (34.12%), Postives = 737/1442 (51.11%), Query Frame = 0

Query:   59 LLTFTFVFPIVRYGTYKPLQQVDLLPVAKRDRAARLLTCLEGEWDMEMRAYKYPSLNRALYRTHRRVFYLCGALLFTEYAIMLTKPVVLRYLLAWLKGTTYIEADVGVYLHWGYGFGVALALAGLDFMQTLVHHRAFYLTIRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAITLGLFFVAPSLAAFVTFLTYWALGNHLELPTVFAVMTLLQTLRECARW-VPRSIEQVSEALVASRRILPLLLMAEAGPSTQNCTEAGSSGGKKEGGGA----KGRKAQWGRRP-SATRAFAEVSSNKSSLLLGSRDPQGKELGARLLPPPPALPPSIPPLPTLTAPPTQPSVGNRGDILVEIEDSGFSWATGNVKGQGIDWQARAEFQLSLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYGSGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKAEEAEEGEGMPKDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFI----------AAEDRILGEVHGDTYASYLRS-GGLASGVWIMLLLLISQGVLTFADFWLKMWASDQDHARTGAYYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLS-------LDLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRT-APVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRE 1475
            LLT++ V  IV +G    L++ D  PV + + A+RL   L  EW+ E R    P++   ++R      Y    LL      + +   +L  LL  +     +  D     + GY +   L LA +  + +      FY  +RTG+ +R  + + +Y+K L+L       +STG IV+ ++ND QR+E+ A    FV  AP+ AL    +L  Y+GV S A G  A  G+IP+Q +  R     R    G  D R++  ++VL G+  VK + WE PF A +  +R SE   L  ++ MRAI    F  +P++    T++TYWALGN L+   +F  ++L    R+     +P++I+ +SE++++  RI   L +    P     TEA  S  +K    A    +     W     S  ++  E   +K S    +R   G   G +   P  +L           APP+ P+           + +G    T + + +G D       ++ LK + + + RG+L+ V GPV +GK++L+  IL E+    G+ V   S  ++AYC Q PWI +GS+R+N++F                                         D+AR+ + ++ CA+  DL     G  + IGE GV LSGGQ+AR +LARA+Y DAD+ LLDDVLSAVDP+V +HLF  CI + L   GK V+L+THQ+ ++  A  + +L+                 S+S  RG               W+    G  EG V +   E +  + +  + D D S P+G               +G+AT R   A +           AEDR +G V GD Y  + +  GGL   V +M LL   + +   AD+WL  W +     +  +  P  Y  I+AV +V+  +R   F+  +LRAS +L  K L +VL APL FF  NP GR+LN+FSKDL   D+LLP T  DFLQ A + V  +V+ C  +P++ L V  L  +F   R+ ++TT+R+VKRL++ TRSP+Y+ V ++L+G  VIRAFR++     RF    DEN    + ++  +R++G+RLD+ CA FL  +  L+V L  +L PG LGL LTY + L G  QWA+RQS + E L+ SVER+  YT L  E     ++             Q   NWP  G + +  + + Y      VL  + +R    +K+G+VGRTG+GKSSFL+AL RL +   G + +DG+   HL   D R S++ IPQ+P LF GTLRFN+DPF ++ DA+L  AL  V L          LD PV +GG N S G RQL+ LARA+L   ++++MDEATA++    D+ IQ ++R+ A +F + T+VTIAHR+NTVI+ D I+V   G+ +E G P  LL       A MV +
Sbjct:   83 LLTYSHVNFIVAHGNKTLLKETDFPPVQEHETASRLSNLLTDEWERE-RKKPNPNVVNVIFRV-----YWPKLLLVIMLGAVYSACRILEALL--IGALVRVFQDSSKSNNEGYMWAGLLTLAVM--LDSTCRGYMFYWAVRTGFNMRVGMISTIYRKTLNLSM--SHASSTGTIVNLVSNDVQRFEDAAPFATFVVSAPIEALVILAILWSYIGVASLA-GFAAIFGIIPIQAFFSRTFARLRTYVVGWRDDRIRNTSDVLLGMNVVKLYAWEEPFRAKLQSLRDSEMLYLTRSSLMRAINDTSFIASPAMVGIATWVTYWALGNELDSSKIFIALSLFNITRQSMTGSLPKAIQFLSESMISINRIQEFLSL----PEMHEETEAPPSDIEKPSSSALVTVRNATFAWNTNTGSFGKSLLENLEDKKSG--KNRTANG---GVKNDNPASSL-----------APPSAPN---------HHQTNGVGLKTTSSREEGSD------LKVILKDVDLNLSRGKLVCVVGPVGAGKTSLIMGILNEMVRTTGT-VQFHSS-KVAYCPQAPWIVSGSVRDNVLFG-------------------------------------YEYDDARFKDTIEMCAMTRDLEIFADGADSLIGERGVTLSGGQKARCSLARAIYYDADIYLLDDVLSAVDPKVGRHLFEKCITE-LKNRGKAVLLITHQLNWVRHADEILVLE---------------HGSISK-RG--------------SWKDVISGDAEGFV-EVLREYQNKDSVDDEID-DESPPEG------------VKHIGKATDRPLHADLREDTKKQKEFVAEDRAIGTVGGDIYWGWFKEGGGLFFVVGLMGLLSGGEAMRVLADWWLSHWTNLTGDEQRSSRQPIIYGIIVAVLIVVVTIRGQFFYVMSLRASNNLSRKMLASVLRAPLWFFQVNPHGRLLNRFSKDLALVDELLPLTFIDFLQCAFIIVGTLVLACTVIPWVTLAVPFLGAIFWYLRKIYMTTSRQVKRLESTTRSPVYSNVPATLEGLSVIRAFRSQERFRRRFYSLQDENTTIFYCWMSCARWVGWRLDLACAVFLGVVAFLSVGLRGSLSPGFLGLLLTYVMGLVGLNQWAVRQSTEVENLMVSVERVLEYTNLPEEPPAVTDL-------------QPPKNWPDKGNIEISQMSLTYPVTKSTVLKDITVRFEPSEKIGLVGRTGAGKSSFLTALFRLVEF-DGSIKVDGLETKHLGLRDLRTSLSIIPQEPFLFKGTLRFNIDPFNKYSDADLWRALDAVELKEKVAGFPEKLDSPVADGGANWSVGERQLICLARAILRDSRLIVMDEATANIDLKTDRLIQQSIRSEAGLFSNATVVTIAHRLNTVIDYDKIMVLDQGRLVEFGRPHELLQNQDGWLARMVHD 1378          

HSP 2 Score: 88.9669 bits (219), Expect = 5.991e-14
Identity = 85/320 (26.56%), Postives = 142/320 (44.38%), Query Frame = 0

Query: 1179 VLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLS-------PNWPHCGELLVKNLQVRYR-EDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDA------ELHAALRDVNLSLD-LDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRETRGVDTAD 1483
            V + TF W     +  ++LL ++E             + K  K R   G  K     S       PN      + +K    R    DL ++L  VD+ +  G+ V +VG  G+GK+S +  +L  N++V       G    H S+      VA+ PQ P + +G++R N+     + DA      E+ A  RD+ +  D  D  +GE G  LS G +   SLARA+     + L+D+  ++V     + +     T    R   ++ I H++N V  +D ILV   G   ++GS + +++     F E++RE +  D+ D
Sbjct:  468 VRNATFAWNTNTGSFGKSLLENLE-------------DKKSGKNRTANGGVKNDNPASSLAPPSAPNHHQTNGVGLKTTSSREEGSDLKVILKDVDLNLSRGKLVCVVGPVGAGKTSLIMGIL--NEMVR----TTGTVQFHSSK------VAYCPQAPWIVSGSVRDNVLFGYEYDDARFKDTIEMCAMTRDLEIFADGADSLIGERGVTLSGGQKARCSLARAIYYDADIYLLDDVLSAVDPKVGRHLFEKCITELKNRGKAVLLITHQLNWVRHADEILVLEHGSISKRGSWKDVISGDAEGFVEVLREYQNKDSVD 762          

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|553186019|ref|XP_005854347.1| (atp-binding sub-family c (cftr mrp) member protein [Nannochloropsis gaditana CCMP526] >gi|422294713|gb|EKU22013.1| atp-binding sub-family c (cftr mrp) member protein [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 664.84 bits (1714), Expect = 0.000e+0
Identity = 337/372 (90.59%), Postives = 349/372 (93.82%), Query Frame = 0

Query:  614 MAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKAEEAEEGEGMPKDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLISQGVLTFADFWLKMWASDQDHARTGAYYPRAYAYIIAVCVVLGLLRADLFFRFALRAS 985
            MAYCSQQPWIQAGSIRENI FAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDS ARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYAD DLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQG MVAPCAPWEEIRTSVSGWR LV DLP+ ATVTGADWETTN GRDEGEVCD+AEEAEEGEG PKDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLISQGVLTFADFWLKMWASDQDHARTGAYYP  +  ++ + +   L+ +   F++A+R S
Sbjct:    1 MAYCSQQPWIQAGSIRENITFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSSARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADPDLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGQMVAPCAPWEEIRTSVSGWRALVQDLPERATVTGADWETTNGGRDEGEVCDEAEEAEEGEGGPKDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLISQGVLTFADFWLKMWASDQDHARTGAYYP--HPGLLGLSLTYALVLSGT-FQWAIRQS 369          

HSP 2 Score: 642.884 bits (1657), Expect = 0.000e+0
Identity = 321/327 (98.17%), Postives = 321/327 (98.17%), Query Frame = 0

Query: 1167 PGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSLDLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRETRGVDTADTAMSKAENLG 1493
            PGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVT KEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDI IRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSLDLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAP FRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRETR  DTADTAMSKAEN G
Sbjct:  345 PGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTRKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIHIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSLDLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPAFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRETRVFDTADTAMSKAENRG 671          

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|167535025|ref|XP_001749187.1| (hypothetical protein [Monosiga brevicollis MX1] >gi|163772340|gb|EDQ85993.1| predicted protein [Monosiga brevicollis MX1])

HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0
Identity = 349/889 (39.26%), Postives = 509/889 (57.26%), Query Frame = 0

Query:  568 SMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYGSGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVT-GADWETTNVGRDEGEVCDKAEEAEEGEGMPKDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLISQGVLTFADFWLKMWA--SDQDHARTGAYYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTA-REVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTR-LSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNL-SLDLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADG 1450
            +++VR  EL+ + G V  GK+T L+ ILGEV C+ GS  H    L  AY  Q PWI AGS+R+NI+F                                  D+P    + ARY+++L+ACAL  D+A LP G+ TEIGE GVNLSGGQ+ARVALARA Y+ A +VLLDD LSAVD  V++HLF   I   L   G+ V+LVTH   ++    R  L++       P A +  + ++++   GL+ ++  +     G   + T++  DEGE                          G  A   +       A G    +     + AEDR  G+V   TY +Y+++GGL   +  ++  L  QG L  +D+WL+ WA  S +D  R    +   Y  +      L L    LFF    RA++SLH++AL  V+  P+ FF +NP GRI+N+F+ DL   D+LLP  ++D L  A     ++VV CIA+P+L +I  P +  +  + R +VT + R +KRL+ +T+SP+YA  S++L G   IR+FRA    EER+L RLDE   C F +++ +RY+GFRLDML    +T   ++ + L  T+DPGLLGL+L YA+ LSG FQ+ +R SAQ ET +TSVER+ +Y R L++E DE+       V  + +    + P WP  G L + +L VRYR+DLP VL+ + + +  G K+G++GRTGSGKSS + ALLRLN I  G +++DG+   ++     R+  A+IPQ PDLFAG++RFNLDP     + E    L+  +L +L LD  V   G NLSTG RQLLSLARA+L RR+++++DEA+A++ +  D+ IQ  LR +  F+ CT++ IAHR+ T+ ++D I+    G
Sbjct:  449 NLQVRASELVGIAGRVGEGKTTFLEGILGEVHCIAGSIHHCGHQL--AYAGQSPWIFAGSLRDNILF----------------------------------DTP---FEAARYASVLEACALVADIAALPNGEDTEIGEKGVNLSGGQKARVALARACYSQAPVVLLDDPLSAVDAHVAEHLFAQAIATFL--QGRTVLLVTHHPHFVARCDRALLIEDHTVREVPVADFANVASTLTVTSGLLANIKASPPADPGTPSDDTSISSDEGEA-------------------------GQSAAIATAKSTTNTAAGADKSKSPHQLVLAEDRKSGDVGSGTYWAYMKAGGLLLVLPSLMGFLFMQGGLMVSDWWLQRWAQQSAEDQDRDSNLH--IYLGLSLGTTALALCNGMLFFSAINRANSSLHEQALARVIGCPMHFFTANPLGRIINRFASDLGQVDELLPIIVYDTLMLAAQAFGSIVVVCIALPWL-IIGVPFVVWYLAHVRAYVTKSLRALKRLEGVTKSPVYAMFSANLAGLNYIRSFRAHEGCEERYLARLDETAACWFWWLLGNRYIGFRLDMLSTCVVTATAIVGILLRDTVDPGLLGLALVYAVALSGLFQFMVRLSAQVETYMTSVERILHYARHLTTESDEEA------VAAQGRALPDVGPAWPDAGMLELHDLSVRYRDDLPDVLHSLSLTMEPGSKIGVMGRTGSGKSSLIQALLRLNVISKGSIIIDGLDTQNVPLSRLRQGFAFIPQTPDLFAGSVRFNLDPKGAFSEHECADVLKQAHLGNLTLDSQVDHQGQNLSTGQRQLLSLARAMLHRRRIVILDEASANIDYDTDELIQRYLRESDYFKGCTIIVIAHRLKTIEDADTIICLKGG 1262          

HSP 2 Score: 169.474 bits (428), Expect = 1.205e-38
Identity = 103/331 (31.12%), Postives = 168/331 (50.76%), Query Frame = 0

Query:   30 ADAALTPPPAPPPPPSPPVDLYERLTPWQLLTFTFVFPIVRYGTYKPLQQVDLLPVAKRDRAARLLTCLEGEWDMEMRAYKYPSLNRALYRTHRRVFYLCGALLFTEYAIMLTKPVVLRYLLAWLKGTTYIEADVGVYLHWGYGFGVALALAGLDFMQTLVHHRAFYLTIRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAIT 360
            AD+A    P     PS     Y+  + +Q +TF++V P+++ G  + L   DL  +AKRD + R++  L   W        +P+L RA  R +R+  +        E A  L +PV++ + + WL+G T    D        YG+G+ +ALA   FMQ + HH  ++ T+R GW +R  +  L++  +L L          G + + ++ D  R+++ ATS+HF W AP+  +    L++  VG  +   G    +  + +  +  ++    RR TA  TD+RVK   EVL+GI TVKA+ WE  F   I+ +R  E +S+  A RM+AI 
Sbjct:   31 ADSASQSEPGAASAPSAEPSPYDVSSFFQRVTFSYVSPLLQIGAKRELAFDDLPALAKRDASERIVKGLLHHWRQLQAETGHPTLLRAAARFYRKEIWWGVWYTLCESAFALIQPVLVFWFVQWLEGNTDYGND--------YGWGIVIALATASFMQAITHHLLYFETMRLGWNMRIGMTGLVHHHLLQLKLSDIPKFGLGAVTNLISTDVLRFDQAATSMHFGWSAPIEVVVVCVLVITEVGWGAGFAGIGVVLLAMAVLTFFSKRFATRRRKTAAFTDERVKSTQEVLNGIGTVKAYTWEQAFGERINGLRKLEARSILKAQRMKAIN 353          

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|1051039470|ref|XP_008834621.2| (PREDICTED: multidrug resistance-associated protein 4 [Nannospalax galili])

HSP 1 Score: 570.852 bits (1470), Expect = 3.242e-177
Identity = 360/943 (38.18%), Postives = 506/943 (53.66%), Query Frame = 0

Query:  563 SLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYG-SGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKA-EEAEE--GEGMP--KDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLISQGVLTF---ADFWLKMWASDQDHARTGA--------------YYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSL-------DLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRE 1475
            +L+ +S   R GELL V GPV +GKS+LL A+LGE+    G   VH R    +AY SQQPW+ +G++R NI+F                                      +  ++ RY  +++ACAL  DL  L  GDLT IG+ G  LSGGQ+ARV LARAVY DAD+ LLDD LSAVD  V KHLF  CI  TL +  K  +LVTHQ+QYL  A+ + +L   G MV                              G   E    G D G +  K  EEAE+    G P  ++     SS    ++ RPS  L      GQ T        + E R  G+V    Y +Y  +G  AS   I+ L+L++     F    D+WL  WA+ Q  A  G               +Y   YA +    V+ G+ R+ L F   + AS +LH+K  +++L AP+ FF  NP GRILN+FSKD+   DDLLP T  DF+Q+  L ++ + +    +P++ + + PL  +F   RR F+ T+R+VKRL++ TRSP+++ +SSSLQG   IRA++AE   +E F    D + +  FLF+  SR+   RLD +CA F+  +   ++ L  TLD G +GL+L+YAL L G FQW++RQSA+ E ++ SVER+  YT L  E   + + +               P WPH G ++  N+   Y  D P+VL  +   I+  +KVGIVGRTG+GKSS +SAL RL++   G + +D +    +  HD R+ ++ IPQ+P LF GT+R NLDPF  H D EL  AL +V L          +D  + E G N S G RQL+ LARA+L + ++L++DEATA+V    D+ IQ  +R    F  CT++TIAHR+NT+I+SD ILV   G+  E   P  LL  + S F +MV++
Sbjct:  264 TLQGISFTARPGELLAVVGPVGAGKSSLLSAVLGELPPTNGLVSVHGR----IAYVSQQPWVFSGTVRSNILFG-------------------------------------KKYEKERYEKVIKACALKKDLQLLEDGDLTVIGDRGATLSGGQKARVNLARAVYQDADIYLLDDPLSAVDAEVGKHLFQLCICQTLHE--KITILVTHQLQYLKAASHILILK-DGEMVQK----------------------------GTYTEFLKSGVDFGSLLKKENEEAEQSPAPGTPTLRNRTFSESSIWSQQSSRPS--LKDGAPEGQDT-ENIQVTQSEESRSEGKVGFKAYKNYFTAG--ASWFIIIFLILLNMAAQVFYVLQDWWLSYWANKQS-ALNGTVNGEGNVTETLDLNWYLGIYAGLTVATVLFGIARSLLVFYVLVNASQTLHNKMFESILKAPVLFFDRNPIGRILNRFSKDIGHMDDLLPLTFLDFIQTLLLVISVIAIAAAVIPWIAIPLVPLAIIFLFLRRYFLETSRDVKRLESSTRSPVFSHLSSSLQGLWTIRAYKAEERCQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAIFVIVVAFGSLILAKTLDAGQVGLALSYALTLMGMFQWSVRQSAEVENMMISVERVIEYTDLEKEAPWEYQKRP-------------PPGWPHEGVIVFDNVNFTYSLDGPLVLKHLTALIKSREKVGIVGRTGAGKSSLISALFRLSE-PEGKIWIDKILTSEIGLHDLRKKMSIIPQEPVLFTGTMRKNLDPFNEHTDEELWNALEEVQLKDAIEDLPGKMDTELAESGSNFSVGQRQLVCLARAILKKNRILIIDEATANVDPRTDELIQQKIREK--FAQCTVLTIAHRLNTIIDSDKILVLDSGRLKEYDEPYVLLQNTESFFYKMVQQ 1112          

HSP 2 Score: 154.451 bits (389), Expect = 4.158e-34
Identity = 87/231 (37.66%), Postives = 129/231 (55.84%), Query Frame = 0

Query:  204 RLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAITLGLFFVAPSLAAFVTFLTYWALGNHLELPTVFAVMTLLQTLR-ECARWVPRSIEQVSEALVASRRILPLLLMAE 433
            RLR A+  ++Y+K L L N      +TGQIV+ L+ND  ++++    LHF+W  PL A+A   LL M +G+ S   G    V ++P Q   G+     R  TA  TD R++ M EV++GI+ +K + WE  F   I+ +R  E   +  ++ +R + L  FFVA  +  FVTF TY  LGN +    VF  MTL   +R     + P +IE+VSEA+V+ +RI   LL+ E
Sbjct:    2 RLRIAMCHMIYRKALRLSNMAMGKTTTGQIVNLLSNDVNKFDQVTIFLHFLWAGPLQAIAVTALLWMEIGI-SCLSGMAVLVILLPFQSCVGKLFSSLRSKTAAFTDARIRTMNEVITGIRIIKMYAWEKSFADLITSLRKKEISKILSSSYLRGMNLASFFVANKVILFVTFTTYVLLGNVITARRVFVAMTLYGAVRLTVTLFFPAAIEKVSEAVVSIQRIKNFLLLDE 231          

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|532025407|ref|XP_005355719.1| (PREDICTED: multidrug resistance-associated protein 4 [Microtus ochrogaster])

HSP 1 Score: 575.089 bits (1481), Expect = 4.693e-177
Identity = 364/944 (38.56%), Postives = 511/944 (54.13%), Query Frame = 0

Query:  563 SLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYG-SGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKAEEAEEGEGMP-------KDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLISQGVLTF---ADFWLKMWASDQ---DHARTGA----------YYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSL-------DLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRE 1475
            +L+ LS   R GELL V GPV +GKS+LL A+LGE+    G   VH R    +AY SQQPW+ +G++R NI+F                                      +  ++ RY  +++ACAL  DL  L  GDLT IG+ G  LSGGQ+ARV LARAVY DAD+ LLDD LSAVD  V KHLF  CI  TL +  K  +LVTHQ+QYL  A+ + +L   G MV                              G   E    G D G +  K  E+EEGE  P       +      SS    ++ RPS  L      GQ T   T A    E R  G+V    Y +Y  +G  AS  +I+ L+L++     F    D+WL  WA+ Q   +H               +Y   YA + A+ V+ G+ R+ L F   ++AS +LH++  +++L AP+ FF  NP GRILN+FSKD+   DDLLP T  DF+Q+  L V+ + V    +P++ + + PL  LF   RR F+ T+R+VKRL++ TRSP+++ +SSSLQG   IRA+RAE   +E F    D + +  FLF+  SR+   RLD +CA F+  +   ++ L  TLD G +GL+L+YA+ L+G FQW++RQSA+ E ++ SVER+  YT L  E   + + +               P WPH G ++  N+   Y  D P+VL  +   I+  +KVGIVGRTG+GKSS +SAL RL++   G + +D +    +  HD R+ ++ IPQ+P LF GT+R NLDPF  H D EL  AL +V L          +D  + E G N S G RQL+ LARA+L + ++L++DEATA+V    D+ IQ  +R    F  CT++TIAHR+NT+I+SD I+V   G+  E   P  LL    S+F +MV++
Sbjct:  427 TLQGLSFTARPGELLAVIGPVGAGKSSLLSAVLGELPPSSGLVNVHGR----IAYVSQQPWVFSGTVRSNILFG-------------------------------------KKYEKERYEKVIKACALKKDLQLLEDGDLTVIGDRGATLSGGQKARVNLARAVYQDADIYLLDDPLSAVDAEVGKHLFQLCICQTLHE--KVTILVTHQLQYLKAASHILILK-DGQMVQK----------------------------GTYTEFLKSGVDFGSLLKK--ESEEGEHSPASGTPTLRKRTFSESSIWSQQSSRPS--LKDGAPEGQDT-ETTQAAQPEESRSEGKVGFKAYKNYFTAG--ASWFFIVFLILLNMAAQVFYVLQDWWLSYWANQQSALNHTENLNESVTETLDLNWYLGIYAGLTAITVLFGIARSLLVFYVLVKASQTLHNRMFESILKAPVLFFDRNPIGRILNRFSKDIGHMDDLLPLTFLDFIQTLLLVVSVIAVAAAVIPWILIPLVPLAILFLILRRYFLATSRDVKRLESTTRSPVFSHLSSSLQGLWTIRAYRAEERFQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAVFVIVVAFGSLILAKTLDAGQVGLALSYAITLTGMFQWSVRQSAEVENMMISVERVIEYTNLEKEAAWEYQKRP-------------PPGWPHEGVIVFDNMSYTYSLDGPLVLKHLTALIKSREKVGIVGRTGAGKSSLISALFRLSE-PEGKIWIDKILTTEIGLHDLRKKMSIIPQEPVLFTGTMRKNLDPFNEHTDEELWNALEEVQLKEAIEDLPGKMDTELAESGSNFSVGQRQLVCLARAILKKNRILIIDEATANVDPRTDELIQQKIREK--FAQCTVLTIAHRLNTIIDSDKIMVLDSGRLKEYDEPYVLLQNPESLFYKMVQQ 1275          

HSP 2 Score: 203.371 bits (516), Expect = 4.505e-49
Identity = 127/397 (31.99%), Postives = 207/397 (52.14%), Query Frame = 0

Query:   42 PPPSPPVDLYERLTPWQLLTFTFVFPIVRYGTYKPLQQVDLLPVAKRDRAARLLTCLEGEWDME-MRAYK---YPSLNRALYRTHRRVFYLCGALLFTEYAIMLTKPVVLRYLLAWLKGTTYIEADVGVYLHWGYGFGVALALAGLDFMQTLVHHRAFYLTIRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAITLGLFFVAPSLAAFVTFLTYWALGNHLELPTVFAVMTLLQTLR-ECARWVPRSIEQVSEALVASRRILPLLLMAE 433
            P P    +L  RL  W L       P+ + G  + L++ D+  V   DR+  L   L+G WD E +RA K    PSL +A+ + + + + + G     E    + +PV+L   + + +   Y  +D  V LH  YG+   L+   L  +  ++HH  FY     G +LR A+  ++Y+K L L N      +TGQIV+ L+ND  ++++    LHF+W  PL A+A   LL M +G+ S   G    V ++PLQ   G+     R  TA  TD R++ M EV++G++ +K + WE  F   I+++R  E   +  ++ +R + +  FF+A  +  FVTF TY  LG+ ++   VF  M+L   +R     + P +IE+VSE++++ RRI   LL+ E
Sbjct:   10 PNPLQDANLCSRLLFWWL------NPLFKTGHSRRLEEDDMFSVLPEDRSKHLGEELQGYWDKEVLRAKKDARKPSLTKAIIKCYWKSYLVLGIFTLIEETTRVVQPVLLGKFIEYFEN--YDPSD-SVALHTAYGYAAILSACTL--LLAVLHHLYFYHVQCAGMKLRVAMCHMIYQKALRLSNAAMGKTTTGQIVNLLSNDVNKFDQVTIFLHFLWAGPLQAIAVTVLLWMEIGI-SCLAGMAVLVILLPLQSCIGKLFSSLRSKTAAFTDARIRTMNEVITGMRIIKMYAWETSFADLITKLRKKEISKILGSSYLRGMNMASFFIANKIILFVTFTTYVLLGHEIKASHVFVTMSLYGAVRLTVTLFFPAAIEKVSESVISIRRIKNFLLLDE 394          

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|731193867|ref|XP_010629058.1| (PREDICTED: multidrug resistance-associated protein 4 isoform X2 [Fukomys damarensis])

HSP 1 Score: 567.77 bits (1462), Expect = 1.285e-175
Identity = 352/942 (37.37%), Postives = 501/942 (53.18%), Query Frame = 0

Query:  563 SLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYGSGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATV-TGADWETTNVGRDEGEVCDKAEEAEEGEGMP-----KDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLIS---QGVLTFADFWLKMWASDQDHARTGA-------------YYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSL-------DLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRE 1475
            +L+ LS  VR GELL V GPV +GKS+LL A+LGE+    G    +    ++AY SQQPW+ +G++R NI+F                                      +  ++ RY  +++ACAL  DL  L  GDLT IG+ G  LSGGQ+ARV LARAVY DAD+ LLDD LSAVD  V KHLF  CI  TL +  K  +LVTHQ+QYL  A+ + +L                               D  TV  G   E    G D G +  K  E  E   +P     ++     SS    ++ RPS  L      GQ T  +       E R  G+V    Y +Y  +G  AS   I+ L+L++   Q      D+WL  WA++Q                    +Y   YA +    V+ G+ R+ L F   + +S +LH+K  +++L AP+ FF  NP GRILN+FSKD+   DDLLP T  DF+Q+    ++ + V    +P++ + + PL  +F   RR F+ T+R+VKRL++ TRSP+++ +SSSLQG   +RA+RAE   +E F    D + +  FLF+  SR+   RLD +CA F+      ++ L  TLD G +GL+L+YAL L G FQW++RQSA+ E ++ SVER+  YT L  E   + + +               P+WPH G ++  N+   Y  D P+VL  +   I+  +KVGIVGRTG+GKSS +SAL RL++   G + +D +    +  HD R+ ++ IPQ+P LF GT+R NLDPF  H D EL  AL +V L          +D  + E G N S G RQL+ LARA+L + ++L++DEATA+V    D+ IQ  +R    F  CT++TIAHR+NT+I+SD I+V   G+  E   P  LL    S+F +MV++
Sbjct:  309 TLRGLSFTVRPGELLAVVGPVGAGKSSLLSAVLGELPPSQG---LVTVQGKIAYVSQQPWVFSGTVRSNILFG-------------------------------------KKYEKERYEKVIKACALKKDLQLLKDGDLTVIGDRGATLSGGQKARVNLARAVYQDADIYLLDDPLSAVDAEVGKHLFQLCICQTLHE--KITILVTHQLQYLKAASHILIL------------------------------KDGETVQKGTYTEFLKSGVDFGSLLKKENEEAEQSSVPGTPTLRNRTFSESSVWSQQSSRPS--LKDGVPEGQDT-EDIQVTQPEESRSEGKVGFKAYKNYFTAG--ASWFIIIFLVLLNLAAQVAYVLQDWWLSYWANEQSLLNITVNGEGNVTEKLDLNWYLGIYAGLTVATVLFGIARSLLVFYVLVNSSQALHNKMFESILKAPVLFFDRNPIGRILNRFSKDIGHMDDLLPLTFLDFIQTLLFVISVMAVAVAVIPWIAVPMIPLAIIFFVLRRYFLETSRDVKRLESTTRSPVFSHLSSSLQGLWTVRAYRAEERCQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAIFVIVTAYGSLILAHTLDAGQVGLALSYALTLMGMFQWSVRQSAEVENMMISVERVIEYTDLEKEAPWEYQKRP-------------PPDWPHEGVIIFDNMNFTYSLDGPVVLKHLTALIKSTEKVGIVGRTGAGKSSLISALFRLSE-PEGKIWIDKILTTEIGLHDLRKKMSIIPQEPVLFTGTMRKNLDPFNEHTDEELWNALEEVQLKEAIEDLPGKMDTELAESGSNFSVGQRQLVCLARAILKKNRILIIDEATANVDPRTDELIQKKIREK--FAHCTVLTIAHRLNTIIDSDKIMVLDSGRLKEYDEPYILLQNKESLFYKMVQQ 1157          

HSP 2 Score: 139.043 bits (349), Expect = 2.512e-29
Identity = 81/236 (34.32%), Postives = 125/236 (52.97%), Query Frame = 0

Query:  199 IRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAITLGLFFVAPSLAAFVTFLTYWALGNHLELPTVFAVMTLLQTLR-ECARWVPRSIEQVSEALVASRRILPLLLMAE 433
            I+  W+    +      + L L N      +TGQIV+ L+ND  ++++    LHF+W  PL A+A   LL M +G+ S   G    + ++PLQ   G+     R  TA  TD R++ M EV++GI+ +K + WE  F   IS +R  E   +  ++ +R + L  FFVA  +  FVTF  Y  LG+ +    VF  MTL   +R     + P +IE+VSEA+++ +RI   LL+ E
Sbjct:   42 IKCYWKSYLVLGIFTLIEALRLSNVALGKTTTGQIVNLLSNDVNKFDQVTIFLHFLWAGPLQAIAVTALLWMEIGI-SCLAGMAVLIILLPLQSCIGKLFSSLRGKTAAFTDARIRTMNEVITGIRIIKMYAWEKSFADLISSLRRKEISKVLRSSYLRGMNLASFFVASKIIVFVTFSCYVLLGHVVTASRVFVAMTLYGAVRLTVTLFFPAAIERVSEAIISIQRIKNFLLLDE 276          

HSP 3 Score: 75.485 bits (184), Expect = 8.367e-10
Identity = 123/513 (23.98%), Postives = 224/513 (43.66%), Query Frame = 0

Query: 1010 GRILNKFSKDLDSADDLLPDTLF-DFLQSAGLCVAAVV--------VGCIAVPYLPLIVAPLL----YLFSKYRRQF-------------VTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGT--FQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLV--KNLQVRYREDLPI-VLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELH-------AALRDVNLSLDLDMPV-GEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDL--------ILVFADGKCIEQGSPQHLLATSGSVFAEMVRE 1475
            G+I+N  S D++  D +   T+F  FL +  L   AV         + C+A   + +I+ PL      LFS  R +              V T   + ++ A  +S  +A++ SSL+  ++ +  R+       +LR ++        F + S+ +          F+TF C + +    T     + ++L  A+ L+ T  F  AI + ++A   + S++R+  +  L    DE  +   +L               P  G+ +V  ++    + + L I  L G+   +R G+ + +VG  G+GKSS LSA+L       G V + G              +A++ QQP +F+GT+R N+  F +  + E +       A  +D+ L  D D+ V G+ G  LS G +  ++LARAV     + L+D+  ++V    D  +   L     F+ C   T+  +I  ++   L        IL+  DG+ +++G+    L  SG  F  ++++
Sbjct:   74 GQIVNLLSNDVNKFDQV---TIFLHFLWAGPLQAIAVTALLWMEIGISCLAGMAVLIILLPLQSCIGKLFSSLRGKTAAFTDARIRTMNEVITGIRIIKMYAWEKS--FADLISSLRRKEISKVLRSS------YLRGMNLAS-----FFVASKII---------VFVTFSCYVLLGHVVTASRVFVAMTLYGAVRLTVTLFFPAAIERVSEA---IISIQRIKNFLLL----DEISQHNLQL---------------PADGKTIVHVQDFTAFWDKTLEIPTLRGLSFTVRPGELLAVVGPVGAGKSSLLSAVLGELPPSQGLVTVQG-------------KIAYVSQQPWVFSGTVRSNI-LFGKKYEKERYEKVIKACALKKDLQLLKDGDLTVIGDRGATLSGGQKARVNLARAVYQDADIYLLDDPLSAV----DAEVGKHL-----FQLCICQTLHEKITILVTHQLQYLKAASHILILKDGETVQKGTYTEFLK-SGVDFGSLLKK 515          

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|348583804|ref|XP_003477662.1| (PREDICTED: multidrug resistance-associated protein 4 [Cavia porcellus])

HSP 1 Score: 570.081 bits (1468), Expect = 2.977e-175
Identity = 354/946 (37.42%), Postives = 503/946 (53.17%), Query Frame = 0

Query:  563 SLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYG-SGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKAEEAEEGEGMP-----KDADMDVSSPDGSEALRPS---GF-LAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLIS---QGVLTFADFWLKMWASDQDHARTGA-------------YYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSL-------DLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRE 1475
            +L+ LS   R GELL V GPV +GKS+LL A+LGE+    G   VH +    +AY SQQPW+ +G++R NI+F                                      +  ++ RY  +++ACAL  DL  L  GDLT IG+ G  LSGGQ+ARV LARAVY DAD+ LLDD LSAVD  V KHLF  CI  TL +  K  +LVTHQ+QYL  A+ + +L   G MV                              G   E    G D G +  K  E  E   +P     ++     SS    ++ RPS   G    Q P   Q T  E       E R  G+V    Y +Y  +G  AS   I+ L+L++   Q      D+WL  WA++Q                    +Y   YA +    ++ G+ R+ L F   + +S +LH+K  +++L AP+ FF  NP GRILN+FSKD+   DDLLP T  DF+Q+  L ++ + V    +P++ + + PL  +F   RR F+ T+R+VKRL++ TRSP+++ +SSSLQG   IRA++AE   +E F    D + +  FLF+  SR+   RLD +CA F+  +   ++ L  +L  G +GL+L+YAL L G FQW++RQSA+ E ++ SVER+  YT L  E   + + +               P WPH G ++  N+   Y  D P+VL  +   I+  +KVGIVGRTG+GKSS +SAL RL++   G + +D +    +  HD R+ ++ IPQ+P LF GT+R NLDPF  H D EL  AL +V L          +D  + E G N S G RQL+ LARA+L + ++L++DEATA+V    D+ IQN +R    F  CT++TIAHR+NT+I+SD I+V   G+  E   P  LL    S+F +MV++
Sbjct:  426 TLQGLSFTARPGELLAVIGPVGAGKSSLLSAVLGELPPSQGLVTVHGK----IAYVSQQPWVFSGTVRSNILFG-------------------------------------KKYEKERYERVIKACALKKDLQLLKDGDLTVIGDRGATLSGGQKARVNLARAVYQDADIYLLDDPLSAVDAEVGKHLFQLCICQTLHE--KITILVTHQLQYLKAASHILILK-DGQMVQK----------------------------GTYTEFLKSGIDFGSLLKKENEEAEPSSVPGTPTLRNRTFSESSVWSQQSSRPSLKDGIPEGQDPENVQVTQSE-------ESRSEGKVGFKAYKNYFTAG--ASWFIIIFLILLNMAAQVTYVLQDWWLSYWANEQSTPNVTVNGKGNVTEKLDLNWYLGIYAGLTVATILFGIARSLLVFYVLVNSSQTLHNKMFESILKAPVLFFDRNPIGRILNRFSKDIGHMDDLLPLTFLDFIQTFLLVISVIAVAIAVIPWIAIPMIPLAIVFFFLRRYFLETSRDVKRLESTTRSPVFSHLSSSLQGLWTIRAYKAEERCQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAVFVIVVTFGSLILAQSLSAGQVGLALSYALTLMGMFQWSVRQSAEVENMMISVERVIEYTNLEKEAPWEYQKRP-------------PPGWPHEGVIIFDNVNFSYSLDGPVVLKHLTALIKSTEKVGIVGRTGAGKSSLISALFRLSE-PEGKIWIDKILTTEIGLHDLRKKMSIIPQEPVLFTGTMRKNLDPFNEHTDEELWNALEEVQLKEAIEDLPGKMDTELAESGSNFSVGQRQLVCLARAILKKNRILIIDEATANVDPRTDELIQNKIREK--FAQCTVLTIAHRLNTIIDSDKIMVLDSGRLKEYDEPYILLQNKDSLFYKMVQQ 1274          

HSP 2 Score: 205.682 bits (522), Expect = 8.291e-50
Identity = 136/408 (33.33%), Postives = 211/408 (51.72%), Query Frame = 0

Query:   42 PPPSPPVDLYERLTPWQLLTFTFVFPIVRYGTYKPLQQVDLLPVAKRDRAARLLTCLEGEWDMEM-RAYK---YPSLNRALYRTHRRVFYLCGALLFTEYAIMLTKPVVLRYLLAWLKGTTYIEADVGVYLHWGYGFGVALALAGLDFMQTLVHHRAFYLTIRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAITLGLFFVAPSLAAFVTFLTYWALGNHLELPTVFAVMTLLQTLR-ECARWVPRSIEQVSEALVASRRILPLLLMAE-AGPSTQNCTE 443
            P P    +L  R+  W L       P+ + G  + L++ D+  V  +DR+  L   L+G WD E+ RA K    PSL +A+ + + + + L G   F E  I + +P+ L  ++ + +   Y    V +Y    YG    L +  L     ++HH  FY    TG RLR A+  ++Y+K L L N      +TGQIV+ L+ND  ++++    LHF+W  PL A+A   LL M +GV S   G    + ++PLQ   G+     R  TA  TD R++ M EV++GI+ +K + WE  F   IS +R  E   +  ++ +R + L  FFVA  +  FVTF +Y  LG+ +    VF  MTL   +R     + P +IE+VSEA+++ RRI   LL+ E + P+ +  TE
Sbjct:   10 PNPLQDANLCSRVFFWWL------NPLFKIGHKRRLEEDDMYSVLPQDRSKYLGEELQGYWDKEVFRAEKDARKPSLTKAIIKCYWKSYLLLGIFTFIEEGIKVIQPLFLGKVINYFEN--YSTDSVALYR--AYGHATVLTICTL--FLAILHHLYFYHVQCTGMRLRVAMCHMIYRKALRLSNVALGKTTTGQIVNLLSNDVNKFDQVTIFLHFLWAGPLQAIAVTVLLWMEIGV-SCLAGMAVLIILLPLQSCIGKLFSSLRSKTAAFTDARIRTMNEVITGIRIIKMYAWEKSFADLISNLRRKEISKVLRSSYLRGMNLASFFVANKIILFVTFTSYVLLGHVITASHVFVAMTLYGAVRLTVTLFFPSAIEKVSEAIISIRRIKNFLLLDEISQPNLEAPTE 404          

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|532062445|ref|XP_005317099.1| (multidrug resistance-associated protein 4 isoform X2 [Ictidomys tridecemlineatus])

HSP 1 Score: 568.155 bits (1463), Expect = 3.140e-175
Identity = 367/984 (37.30%), Postives = 525/984 (53.35%), Query Frame = 0

Query:  520 PTQPSVGNRGDILVEIEDSGFSWATGNVKGQGIDWQARAEFQLSLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYG-SGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLLDAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKAEEAEEGE--GMP--KDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLIS---QGVLTFADFWLKMWASDQDHARTGA-------------YYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSL-------DLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRE 1475
            P  P +   G ++V ++D    W              +A    +L+ LS  VR GELL V GPV +GKS+LL A+LGE+    G   VH R    +AY SQQPW+ +G++R NI+F                                      +  ++ RY  +++ACAL  DL  L  GDLT IG+ G  LSGGQ+ARV LARAVY DAD+ LLDD LSAVD  VSKHLF  CI  TL +  K  +LVTHQ+QYL  A+ + +L   G MV      E +++ V           D A++   +                 EEAE+    G P  ++     SS    ++ RPS  L    A GQ T  +  A    E R  G++    Y +Y  +G  AS   I+ L+L++   Q      D+WL  WA++Q                    +Y   YA +    V+ G+ R+ L F   + +S +LH+K  +++L AP+ FF  NP GRILN+FSKD+   DDLLP T  DF+Q+  + ++ V V    +P++ + + PL  LF   RR F+ T+R+VKRL++ TRSP+++ +SSSLQG   IRA+RAE   +E F    D + +  FLF+  SR+   RLD +CA F+  +   ++ L  TLD G +GL+L+YAL L G FQW++RQSA+ E ++ SVER+  YT L  E   + + +               P WP  G ++  N+   Y  D P+VL  +   I+  +KVGIVGRTG+GKSS +SAL RL++   G + +D +    +  HD R+ ++ IPQ+P LF GT+R NLDPF  H D EL  AL +V L          +D  + E G N S G RQL+ LARA+L + ++L++DEATA+V    D+ IQ  +R    F +CT++TIAHR+NT+I+SD I+V   G+  E   P  LL    S+F +MV++
Sbjct:  322 PHDPQLPPEGKMIVHVQDFTAFWD-------------KASETPTLQGLSFTVRPGELLAVVGPVGAGKSSLLSAVLGELPPSQGLVNVHGR----IAYVSQQPWVFSGTVRSNILFG-------------------------------------KKYEKERYEKVIKACALKKDLQLLEDGDLTVIGDRGTTLSGGQKARVNLARAVYQDADIYLLDDPLSAVDAEVSKHLFQLCICQTLHE--KITILVTHQLQYLKAASHILIL-KDGEMVQKGTYTEFLKSGV-----------DFASLLKKE----------------NEEAEQSPVPGTPTLRNRTFSESSVWSQQSSRPS--LKDGVAEGQDT-EDAQAVQPEESRSEGKIGFKAYKNYFTAG--ASWFIIIFLILLNMAAQVSYVLQDWWLSYWANEQSMLNVTVNGEGNVTEKLDLDWYLGIYAGLTVSTVLFGIARSLLVFYVLVNSSQTLHNKMFESILKAPVLFFDRNPIGRILNRFSKDIGHMDDLLPLTFLDFIQTLLVVISVVSVAVAVIPWIAIPLVPLAILFLFLRRYFLETSRDVKRLESTTRSPVFSHLSSSLQGLWTIRAYRAEERCQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAIFVIVVAFGSLILAKTLDAGQVGLALSYALTLMGMFQWSVRQSAEVENMMISVERVIEYTDLEKEAPWEYQKRP-------------PPTWPQEGVIVFDNVNFTYSLDGPLVLKHLTALIKSREKVGIVGRTGAGKSSLISALFRLSE-PEGKIWIDKILTTEIGLHDLRKKMSIIPQEPVLFTGTMRKNLDPFNEHTDEELWNALEEVQLKETIEDLPGKMDTELAESGSNFSVGQRQLVCLARAILKKNRILIIDEATANVDPRTDELIQKKIREK--FAECTVLTIAHRLNTIIDSDRIMVLDSGRLKEYDEPYVLLQNKESLFYKMVQQ 1200          

HSP 2 Score: 152.91 bits (385), Expect = 1.450e-33
Identity = 87/239 (36.40%), Postives = 128/239 (53.56%), Query Frame = 0

Query:  199 IRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVRASERKSLAWAARMRAITLGLFFVAPSLAAFVTFLTYWALGNHLELPTVFAVMTLLQTLR-ECARWVPRSIEQVSEALVASRRILPLLLMAEAGP 436
            I+  W+    +      + L L N      +TGQIV+ L+ND  ++++    LHF+W  PL A+A   LL M +G+ S   G V  V ++PLQ   G+     R  TA  TD R++ M EV++GI+ +K + WE  F   I+ +R  E   +  ++ +R + L  FFVA  +  FVTF TY  LGN +    VF  MTL   +R     + P +IE+VSEA+V+ RRI   LL+ E  P
Sbjct:   85 IKCYWKSYVILGIFTLIEALRLSNMAMGKTTTGQIVNLLSNDVNKFDQVTIFLHFLWAGPLQAIAVTALLWMEIGI-SCLAGMVVLVILLPLQSCIGKLFSSLRSKTAAYTDARIRTMNEVITGIRIIKMYAWEKSFADLITNLRKKEISKVLRSSYLRGMNLASFFVANKIILFVTFTTYVLLGNVITASRVFVAMTLYGAVRLTVTLFFPSAIERVSEAVVSIRRIKNFLLLDEISP 322          

BLAST of EWM26327.1 vs. NCBI_GenBank
Match: gi|830017886|ref|XP_004680354.2| (PREDICTED: multidrug resistance-associated protein 4 [Condylura cristata])

HSP 1 Score: 568.926 bits (1465), Expect = 7.634e-175
Identity = 361/940 (38.40%), Postives = 513/940 (54.57%), Query Frame = 0

Query:  563 SLKHLSMRVRRGELLIVTGPVRSGKSTLLDAILGEVSCVYGSGVHLRSDLRMAYCSQQPWIQAGSIRENIIFAGVPQRQQETAAPPGNLGVSTPLLPEEDGCIRSNDSPARLVDEARYSNILQACALDHDLAHLPYGDLTEIGELGVNLSGGQRARVALARAVYADADLVLLDDVLSAVDPRVSKHLFHACILDTLVKAGKGVVLVTHQVQYLPLATRVCLL-DAQGHMVAPCAPWEEIRTSVSGWRGLVLDLPDTATVTGADWETTNVGRDEGEVCDKAEEAEEGEGMP-----KDADMDVSSPDGSEALRPSGFLAQTPAVGQATGRETAAFIAAEDRILGEVHGDTYASYLRSGGLASGVWIMLLLLIS-QGVLTFADFWLKMWASDQDHARTGA-------------YYPRAYAYIIAVCVVLGLLRADLFFRFALRASTSLHDKALQAVLAAPLAFFHSNPRGRILNKFSKDLDSADDLLPDTLFDFLQSAGLCVAAVVVGCIAVPYLPLIVAPLLYLFSKYRRQFVTTAREVKRLDAITRSPLYAEVSSSLQGGKVIRAFRAESMMEERFLRRLDENGKCAFLFIILSRYLGFRLDMLCATFLTFLCVLAVALHATLDPGLLGLSLTYALVLSGTFQWAIRQSAQAETLLTSVERLNYYTRLSSEEDEDKEMKTRLVTGKEKTFGQLSPNWPHCGELLVKNLQVRYREDLPIVLNGVDIRIRGGQKVGIVGRTGSGKSSFLSALLRLNQIVGGDVVLDGVSILHLSRHDARRSVAWIPQQPDLFAGTLRFNLDPFMRHGDAELHAALRDVNLSL-------DLDMPVGEGGGNLSTGTRQLLSLARAVLLRRKVLLMDEATASVSFVEDQRIQNTLRTAPVFRDCTLVTIAHRINTVIESDLILVFADGKCIEQGSPQHLLATSGSVFAEMVRE 1475
            +L  LS  VR GELL V GPV +GKS+LL A+LGE+    G    +    R+AY SQQPW+ +G++R NI+F                                      +  ++ RY  +++ACAL  DL  L  GDLT IG+ G  LSGGQ+ARV LARAVY DAD+ LLDD LSAVD  V +HLF  CI  +L +  K  +LVTHQ+QYL  A+++ +L D    +  P A  +  + ++ G  G +++        G   E    G D G +  K  E  E   +P     ++     SS    ++ RPS  L    A GQ       A +  E R  G+V    Y +YL +G     V  +LL+ I+ Q      D+WL  WA+ Q                    +Y   Y+ + A  V+ G+ R+ L F   +R+S +LH+K   ++L AP+ FF  NP GRILN+FSKD+   DDLLP T  DF+Q+  L V  V V    +P++ + + PL  +F   RR F+ T+R+VKRL++ TRSP+++ +SSSLQG  VIRAFRAE   +E F    D + +  FLF+  SR+   RLD +CA F+  +   ++ L  TLD G +GL+L+YAL L G FQW +RQSA+ E ++ SVER+  YT L  E   + + +               P WPH G ++  N+   Y  D P+VL  +   I+  +KVGIVGRTG+GKSS +SAL RL++   G + +D +    +  HD R++++ IPQ+P LF GT+R NLDPF  H D EL  AL++V L          +D  + E G N S G RQL+ LARA+L + ++L++DEATA+V    D+ IQ  +R    F  CT++TIAHR+NT+I+SD I+V   G+  E   P  LL    S+F +MV++
Sbjct:  395 TLHGLSFTVRPGELLAVVGPVGAGKSSLLSAVLGELPPSEGL---VTVQGRVAYVSQQPWVFSGTVRSNILFG-------------------------------------KKYEKERYEKVIKACALKKDLQLLEDGDLTLIGDRGTTLSGGQKARVNLARAVYQDADIYLLDDPLSAVDAEVGRHLFELCICQSLHE--KITILVTHQLQYLKAASQILILKDVPTVLQNPEASLDFSQITLGGGEGKMVE-------KGTYTEFQKSGVDFGSLLKKENEEAEPSPVPGTPTLRNRTFSESSVWSQQSSRPS--LKDGAAEGQEP-ENIQATLPEETRSEGKVGFKAYKNYLAAGAHWLVVVFLLLVNIAAQVAYVLQDWWLSYWANKQSALNVTVNGKANVTEMLHLNWYLGIYSGLTAATVLFGVARSLLVFHVLVRSSQTLHNKMFASILKAPVLFFDRNPIGRILNRFSKDIGHMDDLLPLTFLDFIQTFLLVVGVVGVAVAVIPWIAIPLLPLGIIFFVLRRYFLETSRDVKRLESTTRSPVFSHLSSSLQGLWVIRAFRAEERFQELFDAHQDLHSEAWFLFLTTSRWFAVRLDAICAIFVIVVAFGSLILAKTLDAGQVGLALSYALTLMGMFQWCVRQSAEVENMMISVERVIEYTDLEKEASWECQKRP-------------PPGWPHEGVIVFDNVNFSYSLDGPLVLKHLTALIKSREKVGIVGRTGAGKSSLISALFRLSE-PEGKIWIDKILTTEIGLHDLRKNMSIIPQEPVLFTGTMRKNLDPFNEHTDEELWNALKEVQLKEAIEDLPGKMDTELAESGSNFSVGQRQLVCLARAILRKNRILVIDEATANVDPRTDELIQKKIREK--FAQCTVLTIAHRLNTIIDSDKIMVLDSGRLKEYDEPYILLQNQESLFYKMVQQ 1266          

HSP 2 Score: 139.813 bits (351), Expect = 1.655e-29
Identity = 86/281 (30.60%), Postives = 141/281 (50.18%), Query Frame = 0

Query:   67 PIVRYGTYKPLQQVDLLPVAKRDRAARLLTCLEGEWDME-MRAYK---YPSLNRALYRTHRRVFYLCGALLFTEYAIMLTKPVVLRYLLAWLKGTTYIEADVGVYLHWGYGFGVALALAGLDFMQTLVHHRAFYLTIRTGWRLRTAVQALLYKKVLSLDNRWQSTASTGQIVSTLANDCQRYEECATSLHFVWVAPLMALAGFGLLVMYVGVVSAAVGCVATVGVIPLQVWCGRQVGHARRVTAGRTDKRVKLMAEVLSGIQTVKAFVWEAPFLATISQVR 343
            P+ + G  + L++ D+  V   DR+  L   L+G WD E +RA K    PSL +A+ + + + + + G     E    + +P+ L  ++++ +   Y   D    L   Y +   L    L  +  ++HH  FY     G RLR A+  ++Y+K L L N      +TGQIV+ L+ D  ++++    LHF+W  PL A+A   LL M +G +S   G    + ++PLQ   G+     R  TA  TD R++ M EV++GI+ +K + WE  F   I+  R
Sbjct:   75 PLFKIGHKRRLEEDDMYSVLPEDRSKHLGEELQGYWDKEVLRAEKEAQKPSLTKAIIKCYWKSYLILGIFTLIEEGTKVIQPIFLGKIISYFEN--YDPTDSAA-LQEAYTYATVLTTCTL--VLAILHHLYFYHVQCVGMRLRVAMCHMIYRKALRLSNSAMGKTTTGQIVNLLSGDVNKFDQVTIFLHFLWAGPLQAIAVTALLWMEIG-ISCLAGMAVLLILLPLQSCIGKLFSSLRSKTAAFTDIRIRTMNEVITGIRIIKMYGWEKSFADLITSYR 349