EWM26366.1, cds4303 (CDS) Nannochloropsis gaditana

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|585108376|gb|EWM26366.1| (trna (uracil-5-)-methyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 1722.6 bits (4460), Expect = 0.000e+0
Identity = 840/840 (100.00%), Postives = 840/840 (100.00%), Query Frame = 0

Query:    1 MDSTDGNDTNGDTPAAATDNGTALCDQTAPSTSATEAIALSARQPDQLRLVVSGFQKFMNAKEVTKILAQGKIDYESLKRAVSTTVAVLRFASVEQREKAATDLVKICVKGRPLRTMVPKPEERRIFHQQDHEQCGSKRKGKLNAPREPDGKRGREGSVEGGGDTLMEESVKTLADVVTPFLSLPYMEQLQQKERDIAVECVQKTFREIKKSYADKNKQLKRAYQHGEGGSDSQPRHVRAPNWAWGGRYAKETFVVEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAAIEKQGADWAREQAALVDYLTATTPVGMEGEASTPGEASTPGEASTPGEASTPGEASTPGEAVEGESREVNSFSTTSGPSVSMLHAPSEKETSSNKGDSVVAPHGAWTGVTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFERGDVEIVSENSVQGPDQGEVQGEQRESVTSQVKTET 840
            MDSTDGNDTNGDTPAAATDNGTALCDQTAPSTSATEAIALSARQPDQLRLVVSGFQKFMNAKEVTKILAQGKIDYESLKRAVSTTVAVLRFASVEQREKAATDLVKICVKGRPLRTMVPKPEERRIFHQQDHEQCGSKRKGKLNAPREPDGKRGREGSVEGGGDTLMEESVKTLADVVTPFLSLPYMEQLQQKERDIAVECVQKTFREIKKSYADKNKQLKRAYQHGEGGSDSQPRHVRAPNWAWGGRYAKETFVVEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAAIEKQGADWAREQAALVDYLTATTPVGMEGEASTPGEASTPGEASTPGEASTPGEASTPGEAVEGESREVNSFSTTSGPSVSMLHAPSEKETSSNKGDSVVAPHGAWTGVTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFERGDVEIVSENSVQGPDQGEVQGEQRESVTSQVKTET
Sbjct:    1 MDSTDGNDTNGDTPAAATDNGTALCDQTAPSTSATEAIALSARQPDQLRLVVSGFQKFMNAKEVTKILAQGKIDYESLKRAVSTTVAVLRFASVEQREKAATDLVKICVKGRPLRTMVPKPEERRIFHQQDHEQCGSKRKGKLNAPREPDGKRGREGSVEGGGDTLMEESVKTLADVVTPFLSLPYMEQLQQKERDIAVECVQKTFREIKKSYADKNKQLKRAYQHGEGGSDSQPRHVRAPNWAWGGRYAKETFVVEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAAIEKQGADWAREQAALVDYLTATTPVGMEGEASTPGEASTPGEASTPGEASTPGEASTPGEAVEGESREVNSFSTTSGPSVSMLHAPSEKETSSNKGDSVVAPHGAWTGVTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFERGDVEIVSENSVQGPDQGEVQGEQRESVTSQVKTET 840          

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|698787740|ref|XP_009826902.1| (23S rRNA (uracil-5-)-methyltransferase RumA [Aphanomyces astaci] >gi|574117991|gb|ETV83472.1| 23S rRNA (uracil-5-)-methyltransferase RumA [Aphanomyces astaci])

HSP 1 Score: 225.328 bits (573), Expect = 8.129e-59
Identity = 132/334 (39.52%), Postives = 172/334 (51.50%), Query Frame = 0

Query:  472 GVTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFER 805
             +TS F+  Y G+S P  D P++H++G  ++EEELLGLRFRIS  AFFQVNT GAE LY  V + +          AA+A   + DVCCGTGTIG+C A        AG+                              V+GIE+C++A +DA  NA  N +  A F+ ++AED++ +VLR                      TP    R G+ +  E             QAV                A+VDPPR+GLH   LRALR    + R+VYVSCNP+ SL  +A  LCGP +  LKG AF+PV A PVDMFPHT HCE+++VFER
Sbjct:  561 AITSFFVQEYSGVSAPEEDHPVVHVYGKSHIEEELLGLRFRISANAFFQVNTPGAEALYSLVREFT----------AADAKTQVYDVCCGTGTIGLCLA------ADAGK------------------------------VVGIELCKAATDDAAVNASLNNISNATFINAKAEDVMKNVLR----------------------TP----RQGNDLLLE-------------QAV----------------AVVDPPRAGLHVKVLRALREFNPVTRIVYVSCNPTGSLVENAATLCGPETSNLKGRAFKPVAAAPVDMFPHTEHCEMIIVFER 793          

HSP 2 Score: 126.331 bits (316), Expect = 4.205e-26
Identity = 60/131 (45.80%), Postives = 87/131 (66.41%), Query Frame = 0

Query:  256 VEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAAIEKQGAD-WAREQAALVDYLTA 385
            ++PI PSP LEGYRNKCEF+ G D +  P VGFR G +++G++ VG  +E C+ + A  + +VA  QAF+  + ++PVYD +TH+GVWR +T+R S  T + +VCV A      AD W   +A LV+ LT+
Sbjct:  430 MQPIAPSPALEGYRNKCEFSCGYDADRNPVVGFRFGTYKDGLVSVGAPDE-CINVPATMKAMVATFQAFL-NQSSMPVYDVLTHQGVWRLLTIRASVHTSEVMVCVQANPSALDADAWTAAKAELVEALTS 558          

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|1173946228|gb|OQR94780.1| (tRNA (uracil-5-)-methyltransferase [Achlya hypogyna])

HSP 1 Score: 218.779 bits (556), Expect = 1.469e-56
Identity = 130/334 (38.92%), Postives = 171/334 (51.20%), Query Frame = 0

Query:  472 GVTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFER 805
            G++S+F+  Y G+S P+ D P+ H++G+  +EEELLGL+FRIS QAFFQVNT GAEVLY  V          K   AA+    + DVCCGTGTIGIC A +                                       V+G+E+C++A +DA  NA  N V    F+ S+AED    V++E                                +  +K  G  AE     QAV                AIVDPPR+GLH   LR+LR+ R ++R+VYVSCNP+ SL  DA  LCGP +  L+G+ F+PV A PVDMFPHT HCE+++VFER
Sbjct:  558 GISSMFVQEYTGVSAPAEDHPIDHVYGATTIEEELLGLKFRISAQAFFQVNTPGAEVLYSLV----------KRFAAADDKTQVYDVCCGTGTIGICVASEARK------------------------------------VIGVELCKAATDDAAVNADLNAVKNITFINSKAED----VMKE--------------------------------LLRKKPEGEGAEF--VQQAV----------------AIVDPPRAGLHTKVLRSLRDTRPVKRIVYVSCNPTVSLINDAATLCGPSTSNLRGDPFKPVFAAPVDMFPHTEHCEMIIVFER 791          

HSP 2 Score: 124.02 bits (310), Expect = 2.051e-25
Identity = 62/134 (46.27%), Postives = 84/134 (62.69%), Query Frame = 0

Query:  256 VEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAA-IEKQGADWAREQAALVDYLTATTP 388
            +E I PSP LEGYRNKCEFT G D + +P VGFR+G ++ GV+ VG  E+ C+ +  + + +    Q ++     LPVYD +THEGVWR +T+R S RT Q ++ V A   +   A WA E+ ALV  LTA  P
Sbjct:  426 MERIAPSPVLEGYRNKCEFTFGYDVDKKPVVGFRVGMYKEGVVAVGSPED-CINVPQVMKDICKTFQTYL-ATSTLPVYDVLTHEGVWRLLTIRLSQRTNQIMLMVQANPTDVDAAVWATEKTALVSTLTAAHP 557          

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|1214703303|gb|OWZ15341.1| (23S rRNA (uracil-5-)-methyltransferase RumA [Phytophthora megakarya])

HSP 1 Score: 216.853 bits (551), Expect = 5.752e-56
Identity = 130/337 (38.58%), Postives = 166/337 (49.26%), Query Frame = 0

Query:  473 VTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFERGDVE 809
            +TS+F+  YEGLS PS ++P+ H +G   LEE LLG+RF +SP AFFQVNT GAE LY  V          K    A+   L+ DVCCGTGTIGICA+K                                        V+GIEIC+ A +DA  NA  N V    F+ S+                                            AE+ +  L  + R        D+N         V AIVDPPR+GLH   LRALR+C  ++R+VYVSCNP+ SL RDAV LCGP ++ ++G+AF PV A PVDMFPHT HCE+++VF+R   E
Sbjct:  556 ITSIFMQEYEGLSAPSDNDPVEHAFGKTKLEEHLLGMRFSVSPNAFFQVNTHGAEKLYSLV----------KQYANADEHTLLYDVCCGTGTIGICASK------------------------------------GVGKVVGIEICKPATDDAEVNATLNDVQNVTFVNSK--------------------------------------------AEDVMKDLLRKKR--------DENEKHLNR---VVAIVDPPRAGLHHQVLRALRSCPPVERIVYVSCNPTNSLVRDAVTLCGPSTKSIQGQAFEPVHAAPVDMFPHTQHCEMIIVFDRVKAE 791          

HSP 2 Score: 85.8853 bits (211), Expect = 1.470e-13
Identity = 45/106 (42.45%), Postives = 62/106 (58.49%), Query Frame = 0

Query:  256 VEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCV 361
            ++ I  SP  EGYRNKCEFT G D+E +P +GFR+G FR G + V   +E C+ +    + V A +Q  +     +PVY+  T  GVWR +TVR S  T   +V V
Sbjct:  421 MDSIHASPVTEGYRNKCEFTFGFDKEDKPCLGFRLGLFREGSVVVSKPDE-CINVSNEMKAVCAVMQNIV-ETSDVPVYNVNTKAGVWRVLTVRQSVNTDDLMVMV 524          

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|695409797|ref|XP_009525016.1| (hypothetical protein PHYSODRAFT_496969 [Phytophthora sojae] >gi|348682483|gb|EGZ22299.1| hypothetical protein PHYSODRAFT_496969 [Phytophthora sojae])

HSP 1 Score: 215.312 bits (547), Expect = 2.563e-55
Identity = 128/337 (37.98%), Postives = 170/337 (50.45%), Query Frame = 0

Query:  473 VTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFERGDVE 809
            +TS ++  YEGLS PS ++P+ H++G   LEE LLG+RF +SP AFFQVNT GAE LY  V          K    A+   L+ DVCCGTGTIGICA+K                                      + V+GIEIC+ A +DA  N                     +VL +  V +VS                                 ++++    ++ +L  +   S      V AIVDPPR+GLH + LRALR C  +QR+VYVSCNP+ SL RDAV LCGP ++ ++GEAF+PV A+PVDMFPHT HCE+++VFER   E
Sbjct:  577 ITSFYIQEYEGLSAPSDNDPVEHVFGKTKLEEHLLGMRFDVSPNAFFQVNTRGAEELYSLV----------KQHADADEHTLLYDVCCGTGTIGICASK------------------------------------GVAKVVGIEICKPATDDAEVN---------------------AVLND--VKNVSF--------------------------------INSKAEDVMKDLLRRKREESEKHLNRVVAIVDPPRAGLHHNVLRALRACPPVQRIVYVSCNPTNSLVRDAVMLCGPSTKGMQGEAFQPVHAVPVDMFPHTPHCEMIIVFERVKTE 812          

HSP 2 Score: 86.6557 bits (213), Expect = 1.011e-13
Identity = 53/129 (41.09%), Postives = 71/129 (55.04%), Query Frame = 0

Query:  256 VEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAAIEKQGADWAREQAALVDYLT 384
            ++ I  SP  EGYRNKCEF+ G D+E +P VGFR+G FR G + V   +E CV + +  + V A +Q  +     L VYD  T  GVWR +TVR S  T + +  V   +   G     E+AAL D L 
Sbjct:  442 MDSIHASPVTEGYRNKCEFSFGFDKENKPCVGFRLGLFREGSVVVSKPDE-CVNVSSEMKAVCAVMQNIVETSEIL-VYDVTTKSGVWRVLTVRQSVNTGELMAMV--QVNPAGKT-PEERAALKDLLV 565          

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|693501742|emb|CEF96746.1| ((Uracil-5)-methyltransferase [Ostreococcus tauri])

HSP 1 Score: 206.068 bits (523), Expect = 1.921e-53
Identity = 201/680 (29.56%), Postives = 289/680 (42.50%), Query Frame = 0

Query:  151 GKRGREGSVEGGGDTLMEESVKT---LADVVTPFLSLPYMEQLQQKERDIAVECVQKTFREIKKSYADKNKQLKRAYQHGEGGSDSQPRHVRAPNWAWGGRYAKETFVVEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRG----ALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAAIEKQGADWAREQAALVDYLTATTPVGMEGEASTPGEASTPGEASTPGEASTPGEASTPGEAVEGESREVNSFSTT--SGPSVSMLHAPSEKETSSNKGDSVVAPHGAWT---GVTSLFLAPYEGLSNPSADEPLIHLW--------GSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQA---VLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEA--FRPVKAIPVDMFPHTSHCEVVMVFER 805
            G  G +G  E G D +   + +    + DV+TP     Y EQL  K+     E V ++ REI +   +   + ++A   G   +    R     +        K    +E IV SP L+GYRNK EFT+G   +G  T GF +G+FR+GV+ V     GC  +    + +  A Q ++R R      LPVYD+ T  G WR    R              + E     W R         T         E   P E         P E     EAS P    + E  EV        SG +   +     +E      D+V+      T    V  + +  Y+G+SN + D+  I            S  + E + GL F +S  AFFQVNT  AE LY ++  E     GK          L+LDVCCGTGTIG+     L G V+                                 V+G++I E +++DA  NA+ N V    +L  +AE ++  VLRE    +  + P + A        P  G +      + +L+  S +  AA+QA    ++D+N         V AIVDPPR GLH   L ALR  + ++RLVYVSCN S ++ ++ + LC P      G    F PVKAI +D+FPHT+H E +++ ER
Sbjct:   24 GVVGEDGDAEDGRDVVARANARARRDIRDVITPMWRASYGEQLCAKK-----EIVAESLREITRGVRNACDKARKA---GMAATCDWLRDALGRD--------KLCCEIEGIVRSPVLDGYRNKSEFTIGPSADGEITCGFNVGSFRDGVVAVAP-PVGCRNISETAKYLGDATQTYLRERATAGEGLPVYDKRTATGFWRLFLCREGG--------TAPSSEHGWRHWLRPGTGPPKPPTG--------ENGEPAETENVEPFEYPDE-----EASLPAPTTKDEKSEVMVMFQVRRSGYTEEQI-----REECDGARDAVLEAAAKATPPINVKVIVVQFYDGVSNVAPDDAEIRDMRTFETSEKSSDVIHERMCGLEFSLSATAFFQVNTLAAEALY-RLAGEWASPNGKS---------LLLDVCCGTGTIGM----TLAGNVKK--------------------------------VVGVDIVEESIKDAFVNAQLNGVTNTDWLAGKAEVVIPKVLRE---YNSRIRPVVDA--------PKIGTKRKATSNDPELSDGSDD-EAAIQAAVTTIIDENEYEFDD---VVAIVDPPRGGLHRSVLTALRREKRLKRLVYVSCN-SSTMAKNVIDLCAPQGYDGNGGGAPFAPVKAIALDLFPHTAHVEAIVLLER 598          

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|1173956502|gb|OQS03270.1| (tRNA (uracil-5-)-methyltransferase [Thraustotheca clavata])

HSP 1 Score: 204.527 bits (519), Expect = 1.018e-51
Identity = 125/335 (37.31%), Postives = 165/335 (49.25%), Query Frame = 0

Query:  472 GVTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAE-VRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFER 805
             +TS+++  Y G+S PS D P+ H++G+  +EEELLGL+FRIS QAFFQVNT GAEVLY  V      R    GD        + DVCCGTGTIGIC A                                         V+G+E+C++A +DA  NA  N +    F+ S+AED++  +LR+KP                                     G  AE VR A+   +VD   A   +K L                 R+LR+ R ++R+VYVSCNP+ SL  DA  LCGP +  L+GE F+PV A PVDMFPHT HCE+++VFER
Sbjct:  554 AITSMYMQEYIGVSAPSEDHPIEHVFGATTIEEELLGLKFRISAQAFFQVNTPGAEVLYSLV-----KRFAAAGDSTQ-----VYDVCCGTGTIGICVASDARN------------------------------------VIGVELCKAATDDAAVNAELNNISNITFINSKAEDVMKDLLRKKP------------------------------------EGEGAEFVREAV--AIVDPPRAGLHTKVL-----------------RSLRDTRPVKRIVYVSCNPTVSLINDATTLCGPCTSNLRGEPFKPVFAAPVDMFPHTEHCEMIIVFER 787          

HSP 2 Score: 127.487 bits (319), Expect = 1.586e-26
Identity = 62/134 (46.27%), Postives = 85/134 (63.43%), Query Frame = 0

Query:  256 VEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAAIEKQGAD-WAREQAALVDYLTATTP 388
            +EPI PSP LEGYRNKCEFT G D   +P VGFR+G ++ GV+ VG  ++ C+ +  + + +    QA++     LPVYD +THEGVWR +T+R S RTKQ ++ + A      AD W  E+A L+  LT T P
Sbjct:  422 MEPIAPSPILEGYRNKCEFTFGYDINKKPVVGFRIGMYKEGVVAVGSADK-CINVPQVMKDICRTFQAYL-ESSPLPVYDVMTHEGVWRLLTIRLSERTKQIMLMIQANPSNIEADVWTAEKAKLISTLTQTHP 553          

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|669147756|ref|XP_008609440.1| (hypothetical protein SDRG_05502 [Saprolegnia diclina VS20] >gi|530739036|gb|EQC37278.1| hypothetical protein SDRG_05502 [Saprolegnia diclina VS20])

HSP 1 Score: 203.756 bits (517), Expect = 2.456e-51
Identity = 126/348 (36.21%), Postives = 178/348 (51.15%), Query Frame = 0

Query:  461 DSVVAPHGAWTGVTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFERGDV 808
            +++ A H A   ++S+F+  Y G+S P+ D P+ H++G+  +EEELLGL+FRIS QAFFQVNT GAEVLY  V         K+   A++A + + DVCCGTGTIGIC A       Q  R+                             V+G+E+C++A +DA  NA  N +   +F+ S+AED++  +L++KP                           G    +E +A             +VD   A   +K L                 R+LR+ R ++R+VYVSCNP+ SL  DA  LCGP +  L+G  F+PV A PVDMFPHT HCE+++VFER DV
Sbjct:  553 ETLTAAHPA---ISSMFVQEYTGVSAPTEDHPIEHVFGATTIEEELLGLKFRISAQAFFQVNTPGAEVLYSLV---------KRFAAASDATQ-VYDVCCGTGTIGICVA-------QDARK-----------------------------VIGVELCKAATDDAAVNAELNNIRNISFINSKAEDVMKELLKKKPEG------------------------EGAEFLKEAVA-------------IVDPPRAGLHTKVL-----------------RSLRDTRPVKRIVYVSCNPTVSLINDATTLCGPCTSNLRGTPFKPVFAAPVDMFPHTEHCEMIIVFERVDV 797          

HSP 2 Score: 124.02 bits (310), Expect = 2.245e-25
Identity = 62/134 (46.27%), Postives = 85/134 (63.43%), Query Frame = 0

Query:  256 VEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAAIEKQGAD-WAREQAALVDYLTATTP 388
            +EPI  SP LEGYRNKCEFT G D E +P VGFR+G F+ GV+ VG  +E C+ +  + + +    QA +    ++PVYD +THEGVWR +T+R S RT Q ++ + A      A+ W  E+ ALV+ LTA  P
Sbjct:  429 MEPIAASPILEGYRNKCEFTFGYDVEHKPVVGFRVGMFKEGVVAVGVADE-CINVPQVMKDICRTFQAHL-ETSSMPVYDVMTHEGVWRLLTIRLSQRTNQIMLMIQANPSSVDAEAWTTEKTALVETLTAAHP 560          

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|308799347|ref|XP_003074454.1| (tRNA uracil-5-methyltransferase and related tRNA-modifying enzymes (ISS), partial [Ostreococcus tauri])

HSP 1 Score: 200.675 bits (509), Expect = 3.724e-51
Identity = 203/682 (29.77%), Postives = 286/682 (41.94%), Query Frame = 0

Query:  151 GKRGREGSVEGGGDTLMEESVKT---LADVVTPFLSLPYMEQLQQKERDIAVECVQKTFREIKKS--YADKNKQLKRAYQHGEGGSDSQPRHVRAPNWAWGGRYAKETFVVEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRG----ALPVYDQITHEGVWRYVTVRTSSRTKQCLVCVCAAIEKQGADWAREQAALVDYLTATTPVGMEGEASTPGEASTPGEASTPGEASTPGEASTPGEAVEGESREVNSFSTT--SGPSVSMLHAPSEKETSSNKGDSVVAPHGAWT---GVTSLFLAPYEGLSNPSADEPLIHLW--------GSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQA---VLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEA--FRPVKAIPVDMFPHTSHCEVVMVFER 805
            G  G +G  E G D +   + +    + DV+TP     Y EQL  K+     E V ++ REI +    A     L+ A      G D                  K    +E IV SP L+GYRNK EFT+G   +G  T GF +G+FR+GV+ V     GC  +    + +  A Q ++R R      LPVYD+ T  G WR    R              + E     W R         T         E   P E         P E     EAS P    + E  EV        SG +   +     +E      D+V+      T    V  + +  Y+G+SN + D+  I            S  + E + GL F +S  AFFQVNT  AE LY ++  E     GK          L+LDVCCGTGTIG+     L G V+                                 V+G++I E +++DA  NA+ N V    +L  +AE ++  VLRE    +  + P + A        P  G +      + +L+  S +  AA+QA    ++D+N         V AIVDPPR GLH   L ALR  + ++RLVYVSCN S ++ ++ + LC P      G    F PVKAI +D+FPHT+H E +++ ER
Sbjct:   88 GVVGEDGDAEDGRDVVARANARARRDIRDVITPMWRASYGEQLCAKK-----EIVAESLREITRGAGMAATCDWLRDAL-----GRD------------------KLCCEIEGIVRSPVLDGYRNKSEFTIGPSADGEITCGFNVGSFRDGVVAVAP-PVGCRNISETAKYLGDATQTYLRERATAGEGLPVYDKRTATGFWRLFLCREGG--------TAPSSEHGWRHWLRPGTGPPKPPTG--------ENGEPAETENVEPFEYPDE-----EASLPAPTTKDEKSEVMVMFQVRRSGYTEEQI-----REECDGARDAVLEAAAKATPPINVKVIVVQFYDGVSNVAPDDAEIRDMRTFETSEKSSDVIHERMCGLEFSLSATAFFQVNTLAAEALY-RLAGEWASPNGKS---------LLLDVCCGTGTIGM----TLAGNVKK--------------------------------VVGVDIVEESIKDAFVNAQLNGVTNTDWLAGKAEVVIPKVLRE---YNSRIRPVVDA--------PKIGTKRKATSNDPELSDGSDD-EAAIQAAVTTIIDENEYEFDD---VVAIVDPPRGGLHRSVLTALRREKRLKRLVYVSCN-SSTMAKNVIDLCAPQGYDGNGGGAPFAPVKAIALDLFPHTAHVEAIVLLER 652          

BLAST of EWM26366.1 vs. NCBI_GenBank
Match: gi|301109485|ref|XP_002903823.1| (tRNA (uracil-5-)-methyltransferase [Phytophthora infestans T30-4] >gi|262096826|gb|EEY54878.1| tRNA (uracil-5-)-methyltransferase [Phytophthora infestans T30-4])

HSP 1 Score: 201.445 bits (511), Expect = 1.558e-50
Identity = 127/333 (38.14%), Postives = 166/333 (49.85%), Query Frame = 0

Query:  473 VTSLFLAPYEGLSNPSADEPLIHLWGSPYLEEELLGLRFRISPQAFFQVNTAGAEVLYQKVIDESLGRGGKKGDGAAEAPRLILDVCCGTGTIGICAAKQLEGRVQAGREIAEDPPGTSTQQDNIAALKGQQADVATSLVLGIEICESAVEDAVENARQNRVGCAAFLCSRAEDILLSVLREKPVSSVSVSPPLPASTGNNATTPVSGGRSGHHVAEEKLAGLSAEVRAALQAVLVDQNSASAGSKTLVAAIVDPPRSGLHADCLRALRNCRHIQRLVYVSCNPSKSLPRDAVALCGPPSQRLKGEAFRPVKAIPVDMFPHTSHCEVVMVFER 805
            +TS+F+  YEGLS PS ++P+ H++G   LEE LLG+RF +SP AFFQVNT GAE LY  V          K    A+   L+ DVCCGTGTIGICA+   EG    G+                              V+GIEIC+ A +DA  NA  N V   +F+ S+AED++ S+L++K   S            N     V   R          AGL   V  AL+A                                     C  ++R+VYVSCNP+ SL RDAV LCGP ++ L+G+AF PV A+PVDMFPHT HCE+++VF+R
Sbjct:  581 ITSVFIQEYEGLSAPSDNDPVEHVYGKTKLEEHLLGMRFSVSPNAFFQVNTHGAEKLYSLV----------KQYANADEHTLLYDVCCGTGTIGICAS---EG---VGK------------------------------VVGIEICKPATDDAQVNAILNNVQNVSFVNSKAEDVMKSLLQKKRDES--------EKHLNRVVAIVDPPR----------AGLHHNVLRALRA-------------------------------------CPPVERIVYVSCNPTNSLVRDAVTLCGPSTKSLQGQAFEPVHAVPVDMFPHTPHCEMIIVFDR 812          

HSP 2 Score: 87.0409 bits (214), Expect = 7.255e-14
Identity = 45/106 (42.45%), Postives = 62/106 (58.49%), Query Frame = 0

Query:  256 VEPIVPSPCLEGYRNKCEFTVGRDQEGRPTVGFRMGAFRNGVMQVGGLEEGCVLLDALTQRVVAALQAFIRRRGALPVYDQITHEGVWRYVTVRTSSRTKQCLVCV 361
            ++ I  SP  EGYRNKCEF+ G D+E +P VGFR+G FR G + V   +E C+ +    + V A +Q  +     +PVY   T  GVWR +TVR S  T + +V V
Sbjct:  446 MDSIHASPVTEGYRNKCEFSFGFDKEDKPCVGFRLGLFREGSVVVSKPDE-CINVSKEMKAVCAVMQNIVETSD-VPVYSVTTKSGVWRVLTVRQSVSTGELMVMV 549