EWM26527.1, cds4144 (CDS) Nannochloropsis gaditana

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|585108605|gb|EWM26527.1| (proline dehydrogenase [Nannochloropsis gaditana])

HSP 1 Score: 1244.57 bits (3219), Expect = 0.000e+0
Identity = 599/599 (100.00%), Postives = 599/599 (100.00%), Query Frame = 0

Query:    1 MPLRSLSSLLTRGRAPTANLTRHTLLVPACVAPRFPWCHPLLLHLARAATTTTIPSVSHPPQDAVVPQEPRRSHKIDFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHNRDAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLERMAAGEKLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRRMLSA 599
            MPLRSLSSLLTRGRAPTANLTRHTLLVPACVAPRFPWCHPLLLHLARAATTTTIPSVSHPPQDAVVPQEPRRSHKIDFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHNRDAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLERMAAGEKLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRRMLSA
Sbjct:    1 MPLRSLSSLLTRGRAPTANLTRHTLLVPACVAPRFPWCHPLLLHLARAATTTTIPSVSHPPQDAVVPQEPRRSHKIDFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHNRDAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLERMAAGEKLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRRMLSA 599          

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|298709823|emb|CBJ31621.1| (proline dehydrogenase [Ectocarpus siliculosus])

HSP 1 Score: 487.649 bits (1254), Expect = 3.309e-157
Identity = 253/546 (46.34%), Postives = 351/546 (64.29%), Query Frame = 0

Query:   65 VVPQEPRR--SHKIDFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHA------YKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLN---RLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHNRDAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLERMAAGEKLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRRMLSA 599
            VV +E  R    +++F +   +YAS +  EL+RAY+V +AC+  PLV  ++ LL+I Y +LG++IT +I+  TFFAHFCAGET++ +  R   L+  G+ GILDYAAE D+         + K          +E   ACR Y Y  E  CDA + TFL C+ AV+D TP GFAAIK+TALG+P LL +++ C+    +LF+  D +  G+++ K+F +GY R+F S                  + EE  T LLN   RLDP+N   ID + W   + + ++  ++  CR  GP+  AALSE  + L + M  R+  LA  AA+ GVRLMIDAEQS+ QPAID  +  +    N +   ++TT QCYL+D+ ERL+ +++RA RGG+++A KLVRGAYMVSER+RA E+GYESP+ D IE THA YD  V+  LE MA G++ EV++ATHNQ SIE  +S M +LG+R    GV FGQLLGMAD++T  LG+ GYKAYKY+PYG VG+ +PYL+RRAQENS ++GG  HE+GL+  E+ RR+   
Sbjct:  546 VVEEEQARVAGMRVNFDDASASYASFTDMELLRAYVVLRACAITPLVERSETLLKIGYGVLGESITNAILGKTFFAHFCAGETAEQVGRRAGALKTRGIGGILDYAAEVDLRPAEERAEREAKKREAGDDRLLREEPAACRTYDYMGEAACDARLMTFLTCVRAVRDQTPGGFAAIKLTALGDPVLLSQITTCVHLVRKLFEDFDSDRDGFVSHKDFEEGYRRWFVS------------------ESEEDNTTLLNLMKRLDPNNRGVIDYVGWSNRMRLDNLAEISAHCRIPGPMAAAALSENGVALKKTMYRRIATLATAAASSGVRLMIDAEQSWLQPAIDNEVYSLQLEFNTEFPTIFTTYQCYLKDSLERLKTDLDRAARGGYVWAGKLVRGAYMVSERERAKEEGYESPVFDAIEETHANYDAAVTLVLEHMAKGQRAEVMMATHNQKSIEHAISLMQKLGIR-RQDGVSFGQLLGMADNITFPLGTGGYKAYKYIPYGKVGETIPYLLRRAQENSGMLGGASHERGLLVKEIRRRVFGG 1072          

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|633913371|gb|KDD76966.1| (Proline dehydrogenase [Helicosporidium sp. ATCC 50920])

HSP 1 Score: 466.463 bits (1199), Expect = 2.385e-155
Identity = 239/529 (45.18%), Postives = 331/529 (62.57%), Query Frame = 0

Query:   69 EPRRSHKIDFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHNRDAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLERMAAGEKLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRRML 597
            E R +  + F + K A+ + S  +LVR+  VF  C  +PLV H++ +L+ S RL    + +++VR TF+  F AG   + I+PR++ L   G+  ILDYAAE D+ +      ++P         +  R Y+YE E  CD  +  FL  I A      +GFAAIK+TALG P LLER+SV +    RLF  +D +G G +T  +F++G+   F                   V+ +    ++  R+D     R+D +DW   V++ DM ++   CRE+GP   A L+++E  L++AM  R++ LA  A   GVRLM+DAE SYFQP ID ++  +   HNR A V+Y T QCYL+D+ ERLE+++ERARRGG+ F AKLVRGAYMV ER+RA+++G ESPI +TIE THA+YDG V+  L+ +A     E+LVA+HNQ SIE+ + GM   GL   +SGVYFGQLLGMAD+LT  LG  GY AYKYVP+G V +V+PYLIRRAQENSD++GGV  E  ++R ELWRR L
Sbjct:   46 ESRSALLLPFDDPKEAFKAKSDLDLVRSLAVFSLCRIQPLVKHSEGILKWSRRLFSAPLVDAVVRATFYRQFVAGANGEDIKPRLKYLADNGIRAILDYAAEDDV-EADKDGSSEPNEM------VVVRTYAYEGEALCDKRMGIFLKSIEASASADGQGFAAIKVTALGVPTLLERVSVALVAIKRLFASLDADGDGSVTEDQFVEGWRSIFH------------------VEDDAKVREIFRRIDSSEDGRVDYVDWSSKVSVFDMASIAAHCRESGPFVAAVLTQEEQHLLKAMIERVDTLAKAAGAHGVRLMVDAEHSYFQPVIDAVVSHLQADHNRAAPVLYNTYQCYLKDSHERLELDLERARRGGYKFGAKLVRGAYMVLERRRASQQGAESPILETIEETHASYDGAVNVLLDDIARETGTELLVASHNQRSIERCVEGMAARGL-SPSSGVYFGQLLGMADNLTFVLGRHGYGAYKYVPFGDVAEVMPYLIRRAQENSDMLGGVAKELSMMRTELWRRWL 548          

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|223996733|ref|XP_002288040.1| (proline dehydrogenase, partial [Thalassiosira pseudonana CCMP1335] >gi|220977156|gb|EED95483.1| proline dehydrogenase, partial [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 432.18 bits (1110), Expect = 4.308e-142
Identity = 230/554 (41.52%), Postives = 331/554 (59.75%), Query Frame = 0

Query:   78 FGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADIT------------------------------QMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHN----RDAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFL-ERMAAGEKLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRRM 596
            F +   A+ + ST E++R+  VF+AC    LV +A++LL +S ++LG  IT ++++ TFF HFCAGE S  ++P I++L+   +  ILDYAAE++ +                              Q   S L+              R+Y Y +E++CD HVE F +CI +V+DV+P GFAA+K+TALGNP LLERMS  I E  + F  MD +  G+ITR+EFL  YDR F                    + ++  +++L+ LD DNT  ID I +   +    +P+ T +C++ GPL  A  S  E+ LM  M  RL  LA  AA  G +L+IDAE + +QPAID L+L++ Q+ N     D  V++ T QCYL+D  ER+  +++R+ R  F FAAKLVRGAYMV ER+RA + GY+SPIHDT + TH  Y+  V   L  RM  G  +E+++ATHN+ SIEK +S M+ +GL    + V+F QL GM+D+LT +LG++GY A+KY+PYG V +VLPYL+RRAQEN D++G    E  L+  EL +R+
Sbjct:    1 FSDPASAHGTKSTTEIIRSIAVFQACRIPFLVKNAERLLDLSSKVLGNTITNTLMKYTFFRHFCAGEDSVDMKPVIDKLQANNIGPILDYAAESEGSDDGDDVPAIVFEGKRCYILYNMHLYLQIWYQQQYSFLSPTGISTQPLFNQPARIYDYTTEKECDRHVEIFKNCIRSVRDVSPHGFAALKVTALGNPELLERMSTIIVEVKKFFALMDEKKSGFITREEFLTCYDRHFHR------------------EDQDALSEVLDFLDADNTGMIDYISFAKMLTPYTLPSFTLKCKDVGPLAMATPSVDEIALMVKMSERLNTLAEEAAACGTKLLIDAEHAKYQPAIDSLVLELQQKFNAKDVTDRPVIFNTYQCYLKDMPERMITDLKRSERYNFHFAAKLVRGAYMVHERERAKKLGYDSPIHDTAQDTHRCYNEVVELLLRHRMQHGPGVEMMIATHNKESIEKAVSLMNEIGLYPNDATVHFAQLYGMSDNLTFTLGNSGYNAFKYLPYGEVQEVLPYLLRRAQENGDMLGNAGTEVSLLGRELKKRL 536          

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|397610345|gb|EJK60781.1| (hypothetical protein THAOC_18807 [Thalassiosira oceanica])

HSP 1 Score: 433.335 bits (1113), Expect = 2.300e-141
Identity = 234/528 (44.32%), Postives = 326/528 (61.74%), Query Frame = 0

Query:   76 IDFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEAD--ITQMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHNR----DAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLE-RMAAGEKLEVLVATHNQASIEKTLSGMDRLGLR-GETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRR 595
            IDF ++  A++  +T EL+RA  VF+ C  + +V +A  LL +S R+    ITE+IV  TF+ HFCAG  S  ++P I+ L++ GV  ILDYAAE +   +    SP        +       R+Y+Y+SE  CD HVE F DCIH+V+DV+P GFAA+K+TALGNP LLERMS  I ET RLF K D    G +T ++F + Y ++FR+                  + +E+A +++  +D DN   ID I++   ++   +P  T QC + GPL  A  S++E+ LM+ M  RL  LA  AA  G +L+IDAE   +QPAID L+L++ Q+ N     D  V++ T QCY++DA ER ++++ER+ R GF FAAK+VRGAY+  ER RA E GYESPIHD+ E T   YD  V   L  R   G  LEV+VA+HNQ SIE+ +  M RLG++  +   V+F QL GM D+LT +LG A + A+KY+PYG VG+V+PYL+RRA+ENS V+     E  L+  EL RR
Sbjct:  125 IDFSDSSTAHSQKTTFELLRALAVFRVCQVKWIVDNAKMLLNVSNRI-NTTITEAIVERTFYKHFCAGRDSVDMKPVIDMLQRHGVRPILDYAAENEGGESDEGISPQDAEDILTHPPFNQPARIYAYKSEEDCDRHVEIFRDCIHSVRDVSPCGFAALKVTALGNPELLERMSTMIVETKRLFSKFDTGKTGMVTHEQFKECYRQYFRA------------------EDDEIA-KIIQDIDLDNNGMIDYIEFAEMMHPCQLPTFTKQCTDVGPLALATPSDEEIALMQRMSERLHTLAQEAADCGTKLLIDAEHLKYQPAIDSLVLELQQKFNDKGKTDRPVIFNTYQCYMKDAAERAQVDLERSERFGFHFAAKIVRGAYLHHERSRAEELGYESPIHDSKEETDRCYDEVVELLLRYRSEHGPGLEVMVASHNQGSIERAVHLMQRLGIQCADNPSVHFAQLFGMCDNLTFTLGKANFNAFKYLPYGPVGEVIPYLLRRAEENSSVLDKTGDEIHLLLDELKRR 632          

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|167517605|ref|XP_001743143.1| (hypothetical protein, partial [Monosiga brevicollis MX1] >gi|163778242|gb|EDQ91857.1| predicted protein, partial [Monosiga brevicollis MX1])

HSP 1 Score: 424.091 bits (1089), Expect = 2.517e-139
Identity = 226/505 (44.75%), Postives = 306/505 (60.59%), Query Frame = 0

Query:   76 IDFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGP------LKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHNRDAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLERMAAGEKLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQEN 574
            +DF NT+ A+   S  +L+ AY V+KAC F PLV HAD + ++S R LG  IT+ +VR TFF HFC GE +  +      L+  G+  I+DYAAEAD+ +       KP   +  + + + RVY Y SE QCD +V+ FLDCI +V  VTP G AAIKITALG P L+ERM+V I  T  LF  +D +  G ++  EF      FF                     P+  A ++ + +D D+   +D I+W  ++ + D+  L    R  GP      L+ A L E E+ELMR M  RL  L   AA   VRLM+DAEQ+YFQPAI  L L + +++N++  +VY T QCYL+D    LE +++RARR  + FA KLVRGAYM  ER+ A++KGY SPI DT + TH TY+  ++  L +    ++  +LVA+HNQASIE T+S M + G+  +T GV F QLLGM DHLT +LG  GYKA+KYVPYG V +V+PYL+RRA EN
Sbjct:    1 LDFHNTQTAFQDKSLTDLLLAYAVYKACMFSPLVKHADSVYKLSRRFLGPRITDGVVRATFFRHFCGGENNNDLLETTTSLKNSGIGAIMDYAAEADVVK-------KPDVVSEVKAKSSARVYDYISEAQCDRNVQHFLDCIESVHQVTPGGIAAIKITALGLPDLIERMAVAITRTQWLFSLIDSDNSGTLSADEFEKAVATFF---------------------PDIDAKEVFHAIDTDSDSEVDYIEWTHSLRMEDVWNLI---RSAGPNTPLGELQEAMLDETELELMRNMMRRLHTLVDYAAERKVRLMVDAEQTYFQPAIHNLALGLQRKYNKELPLVYNTYQCYLKDTLSTLEHDLQRARRENWWFACKLVRGAYMEMERQLASDKGYPSPICDTQDDTHRTYNTSINMILNQ----DRANLLVASHNQASIEYTISEMQKRGIDKQTGGVCFAQLLGMCDHLTYTLGINGYKAFKYVPYGPVNEVIPYLVRRAHEN 470          

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|219121368|ref|XP_002185909.1| (predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1] >gi|209582758|gb|ACI65379.1| predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 418.698 bits (1075), Expect = 2.757e-137
Identity = 229/529 (43.29%), Postives = 308/529 (58.22%), Query Frame = 0

Query:   78 FGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHAYKEGEI-----ACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHNRDAF----VVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLERMAAGEK-LEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRRM 596
            F + + AY S +  EL+RA L F  C    LV ++++LL++  +++G  IT+ I++ T F HFCAGE  + I P I +L K G+  ILD+AAE D          + K     +  I       RVY YESE +CD HVETF  CI  V D+  +G+AAIK+TAL NP LLERMS  I E   LF K D  G G +T+ EF  G++ FF+   A                   M    L+ L   +   +D I W M +  SD+P LT  CRE GPL  AA +++E+ELM  M  R   LA +AA +G RL+IDAEQ+ FQPAID  +L++ + +N        V+Y T QCYL+D  +RL  ++ER+ R  + F AKLVRGAYM SER  A   G+ SPI DTI+ TH  Y+  V   L+     +K  E+++ATHNQ SIEK +  M+  G+  +   + FGQL GM+D+LT +LG  GY+AYKYVPYG V  V+PYLIRRA ENS +  G   E  +I+ EL RR+
Sbjct:    1 FNDAEAAYESKTNPELIRAMLSFGLCQIPILVRYSERLLKLGRKIVGDTITDGILKQTLFGHFCAGEDEKQILPAIHKLEKAGIGSILDFAAEDDGEGDDGDDGDRSKKEKIADEIIQDKSPKVRVYDYESEAKCDRHVETFHKCIRDVADLGKDGYAAIKVTALTNPKLLERMSRAIVEAQNLFAKFDENGDGIVTKDEFKHGFNLFFKGEDA-------------------MLNDALDHLQLADGS-VDYITWSMLLTPSDLPRLTAGCREVGPLSLAAPTDEEIELMERMFERGHELARQAAEVGTRLLIDAEQARFQPAIDNFVLNLQRTYNATGVSEFPVIYNTYQCYLKDVPDRLRTDVERSERFDYHFGAKLVRGAYMESERALAKSLGFPSPIQDTIQDTHDCYNNSVDFLLKHAIQSDKTCELMLATHNQESIEKAIHSMNEHGVNRKDPTICFGQLFGMSDNLTFNLGRHGYRAYKYVPYGEVKMVMPYLIRRANENSSIASGAAQELRMIQNELKRRV 509          

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|219121003|ref|XP_002185733.1| (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >gi|209582582|gb|ACI65203.1| predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 416.772 bits (1070), Expect = 1.138e-136
Identity = 227/524 (43.32%), Postives = 307/524 (58.59%), Query Frame = 0

Query:   77 DFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHN---RDAF-VVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLE-RMAAGEKLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRR 595
            +F + K+AYA+ S  EL RA L F  C  + LV +A+ LL +S  LLG   T++ ++ T + HF AGE    I+P++  L   G+  ILDYAAE +   ++T     P T      +   RVY Y  E +CD H+ETF  CI AV ++  +G+AA+KITAL NP LLERMS  I E   LF K D  G G I+R EF   +  FF+      S                    +L  L PD    +D I W M +   D+P +   CRE GPL  AA +++E+EL+ +M  R   LA  AA  G  ++IDAEQ  +QPAID L+LD+ +  N      F +VY T QCYL+D+ ERL+ ++ER+ R  + F AKLVRGAY+ SER+ A +    SP++  +E TH  Y+  V   LE    + +K+E+++ATHNQ SIEK +  M+RLG+  + S V FGQLLGMADHL+ +LG  GY+AYKYVPYG V + +PYLIRRA ENS + GG   E  +I  EL RR
Sbjct:    3 NFADAKIAYATQSNMELGRALLSFSLCQIKTLVKYANPLLTLSRFLLGDRFTDAALKATLYGHFVAGEDEVRIQPKVRVLEYAGIGSILDYAAEEEGQGVVT-----PATSVDPASQPIARVYDYTCEAKCDRHLETFKSCIQAVANLEKDGYAAVKITALTNPDLLERMSKAIVEARNLFAKFDSNGDGMISRHEFAQTFRVFFKGEDTKLS-------------------NMLEELQPDQNGNVDYIMWSMMLAPGDLPRICAGCREVGPLALAAPTDEEVELIESMFRRGHALAQEAAQCGSSVLIDAEQYRYQPAIDNLVLDLQRTFNATSESKFPIVYNTYQCYLKDSMERLKTDLERSERFDYHFGAKLVRGAYLESERELAEKAKVPSPVYTELEETHQCYNEAVEFLLEYSTQSNKKVELMLATHNQESIEKAIEVMNRLGVHRQDSTVSFGQLLGMADHLSFNLGRHGYRAYKYVPYGEVKEAMPYLIRRANENSSLAGGASREMAMIGQELKRR 502          

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|514682490|ref|XP_004988828.1| (proline oxidase [Salpingoeca rosetta] >gi|326434030|gb|EGD79600.1| proline oxidase [Salpingoeca rosetta])

HSP 1 Score: 410.994 bits (1055), Expect = 1.600e-132
Identity = 230/560 (41.07%), Postives = 320/560 (57.14%), Query Frame = 0

Query:   46 ARAATTTTIPSVSHPPQDAVVPQEPRRS-HKIDFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTPEGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDG-----YDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAAL---SEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHNRDAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLERMAAGEKLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRRM 596
            A  A T  +   S PP D   P     S  K+DF +   A+ + S  +LV AY V K C+ R +V +A  L + +  + G  + + IV+ TFF HFCAGE    +RP + ELR  G+  I+DYAAEAD+         KP+    +E  ++ R Y Y  E  CDA+VE F  CI  V +VTP G AAIK+TALG+P LL+++S  I +T  LF    +  +G        DG     + RFF       S+              +      N +D D   R+D ++W   ++I D+  L  +     PL +      +E+E+EL+R +Q RL  +   A    VRLMIDAEQ+YFQP ID L+L + +++N+   ++Y T QCYL+DA  R+  ++ERA+R G+ FAAKLVRGAYM +ERK A ++GY  PIHDTIE THA Y+  +   ++      +  VL+A+HNQ S+E  +  M R G+  +T GV F QL GM+DHLT +LG  GYKAYKYVPYG V +V+PYLIRRA ENS ++GG  HE  +I  E+ RR+
Sbjct:  102 AGQAPTPAVDPTSAPPHDTGAPAHATMSADKLDFSDPSQAFQTKSLYDLVLAYGVLKLCTVRQIVENAGPLYKKTRAVFGARLVDGIVKGTFFRHFCAGENEDELRPIVAELRASGIGSIMDYAAEADLED-------KPEDVLEEEQRLSARTYDYIDESHCDANVENFKTCIENVHNVTPAGIAAIKVTALGHPDLLKQLSTAIIQTQWLFDLFAKREEG--VEHHHADGLTEEDFQRFFHQYFPDISD--------------QEIHDFYNLVDSDRNGRVDFVEWTHNLHIEDIWELMRRAGPHTPLAHITHEMPTERELELLRNLQRRLTEVVEYAEKHRVRLMIDAEQTYFQPIIDNLVLGLQRKYNKVYGLIYQTYQCYLKDAVLRITHDLERAQREGWWFAAKLVRGAYMEAERKLAEQRGYPDPIHDTIEDTHAAYNRAIQLLMD----FPRCNVLIASHNQDSVEFAVEEMKRRGIDPQTGGVCFAQLRGMSDHLTYTLGLNGYKAYKYVPYGPVNEVMPYLIRRAHENSGMLGGASHELTMISNEIKRRI 634          

BLAST of EWM26527.1 vs. NCBI_GenBank
Match: gi|1072232920|gb|OEU17651.1| (FAD-linked oxidoreductase [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 408.683 bits (1049), Expect = 1.682e-132
Identity = 219/524 (41.79%), Postives = 306/524 (58.40%), Query Frame = 0

Query:   77 DFGNTKVAYASVSTGELVRAYLVFKACSFRPLVTHADKLLQISYRLLGQAITESIVRNTFFAHFCAGETSQTIRPRIEELRKGGVAGILDYAAEADITQMMTSPLTKPKTHAYKEGEIACRVYSYESERQCDAHVETFLDCIHAVKDVTP--EGFAAIKITALGNPHLLERMSVCIEETDRLFKKMDREGKGWITRKEFLDGYDRFFRSTPAPPSETSPVPTGIWAVKPEEMATQLLNRLDPDNTDRIDVIDWRMAVNISDMPALTHQCRETGPLKNAALSEQEMELMRAMQLRLEMLAGRAATLGVRLMIDAEQSYFQPAIDFLILDVMQRHN-RDAFVVYTTMQCYLRDATERLEMEIERARRGGFLFAAKLVRGAYMVSERKRAAEKGYESPIHDTIEATHATYDGCVSTFLERMAAGE-KLEVLVATHNQASIEKTLSGMDRLGLRGETSGVYFGQLLGMADHLTLSLGSAGYKAYKYVPYGLVGQVLPYLIRRAQENSDVMGGVKHEQGLIRAELWRRM 596
            DF + ++ Y   ST EL+RA   F+ C    LV +A+ +L +  +++G  I + I++ T + HFCAGE  + IRP ++ L   GV  ILDYAAE D     T+           E  I  R Y YESE +CD H  TF+ CI  V  + P  +G+AA+K+TALGNP LL R+S  I E  RLF+  D +G G ++RKEF  GY+R+F               G   +K      ++  + DP  T  ID I W M +   D+P +  +C   G L +A  +++E+EL+ AM  R  +L   AA  GVRL+IDAEQ  +QPAID L+L++ +  N  D  ++Y T QCYL D+ ERL+ ++ER+ R  + F AKLVRGAYM SER+ A   G   PIH TIE TH  Y+  V+  L   A  +  +E++ ATHN  SI K +  M+  G+   +S + F QL GM+D LT +LG  GY+A+KYVPYG V +V+PYL+RRA+ENS ++G    E  +I+AEL RR 
Sbjct:   65 DFNDARITYEDKSTTELLRAAGCFRLCQVPFLVNYAESMLWLGRKIVGGRIVDMILKATLYGHFCAGEDQERIRPVLQSLANAGVGSILDYAAENDSGDGETTESA--------EFNITAREYDYESEAKCDKHTSTFIKCIKDVGALNPNHDGYAAVKVTALGNPKLLARLSTAIVEAKRLFEVFDLDGDGLLSRKEFEVGYNRYF-------------ADGDTRIK------KIFEKFDPLQTGYIDYITWSMMLQPQDLPKICKRCNTAGRLSDACPTDEEIELLNAMYDRGRLLGEEAAKSGVRLLIDAEQKRYQPAIDNLVLELQREFNGSDKPIIYNTYQCYLVDSPERLKNDVERSERFNYHFGAKLVRGAYMESERELAEAVGDPDPIHPTIEKTHECYNNSVAYLLRHSAKSDLNVEIMCATHNDESITKAIESMNDYGIDRTSSTLCFAQLYGMSDALTFNLGKYGYRAFKYVPYGEVDEVMPYLLRRARENSAIVGATTFELEMIKAELGRRF 561