EWM26995.1, cds3674 (CDS) Nannochloropsis gaditana

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|585109165|gb|EWM26995.1| (mannitol dehydrogenase [Nannochloropsis gaditana])

HSP 1 Score: 1503.42 bits (3891), Expect = 0.000e+0
Identity = 724/724 (100.00%), Postives = 724/724 (100.00%), Query Frame = 0

Query:    1 MTTDMKIPELPKREGVEPEWNMDLDALDLHIPQPVINIEIEANGAITLTKTDPHELQRKANKFRQGYKNWRQKKPFGARGEAKDLKAQREEEAAEEKEEEPAPKLQHGRTISSMEPTASGVEASVGSGTGHEVIIPLSPVKGLARRKSFSVSLSAQDLTAEAEENWLKFYVNPFAELLDKKVGPLPLCTKNLETILHRNFKSVRGKGPSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPDDRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQKVDGNLNTEDPNVKHDLSDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYMEEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSSGSESYDPKEVISIVFGPEIAKNKAFVGTVKEALESICKRGIMYTLLHVN 724
            MTTDMKIPELPKREGVEPEWNMDLDALDLHIPQPVINIEIEANGAITLTKTDPHELQRKANKFRQGYKNWRQKKPFGARGEAKDLKAQREEEAAEEKEEEPAPKLQHGRTISSMEPTASGVEASVGSGTGHEVIIPLSPVKGLARRKSFSVSLSAQDLTAEAEENWLKFYVNPFAELLDKKVGPLPLCTKNLETILHRNFKSVRGKGPSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPDDRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQKVDGNLNTEDPNVKHDLSDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYMEEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSSGSESYDPKEVISIVFGPEIAKNKAFVGTVKEALESICKRGIMYTLLHVN
Sbjct:    1 MTTDMKIPELPKREGVEPEWNMDLDALDLHIPQPVINIEIEANGAITLTKTDPHELQRKANKFRQGYKNWRQKKPFGARGEAKDLKAQREEEAAEEKEEEPAPKLQHGRTISSMEPTASGVEASVGSGTGHEVIIPLSPVKGLARRKSFSVSLSAQDLTAEAEENWLKFYVNPFAELLDKKVGPLPLCTKNLETILHRNFKSVRGKGPSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPDDRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQKVDGNLNTEDPNVKHDLSDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYMEEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSSGSESYDPKEVISIVFGPEIAKNKAFVGTVKEALESICKRGIMYTLLHVN 724          

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|510951779|gb|AGN55416.1| (mannitol dehydrogenase [Saccharina japonica])

HSP 1 Score: 527.324 bits (1357), Expect = 8.744e-176
Identity = 299/678 (44.10%), Postives = 413/678 (60.91%), Query Frame = 0

Query:   52 DPHELQRKANKFRQGYKNWRQKKPFGARGEAKDLKAQREEEAAEEKEEEPAPKLQHGRTISSMEPTASGVEASVGSGTGHEVIIPLSP-VKGLARRKSF---SVSLSAQDLTAEAE-ENWLKFYVNPFAELLDKKVG-PLPLCTKNLETILHRNFKSVRGKGPSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPD-DRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQKVDGNLNTEDPNVKHDL-SDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYMEEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTG-IDQEGEEIEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSSGSESYDPKEVISIVFGPEIAKNKAFVGTVKEALESICKRGIMYTL 720
            DP  L++KA  FR+GY NWR KK  GARGE      + +  A + K EE    +     ++S+E   +                P +P  +G++RR S    +  +   DL A  E ENWLKFYVNP++ + + ++  P+ L   NLE ++       +   P   R    Y  + H+GVGGFHRSHQAV+TDDLL L    +A NG       P D  +W I GIGLM WD KMY  L  QDH+YT+L R   G++AR+VGSI DF+FAP+++QA+   L    TRIVSLT+TEKGYCQ V+G+L+  +  VK DL  DL+ P +A+GM+VAAL +R++ G  SFTVLSCDN P+NG +++ +V +MA   V ++L  W+  +TTFPNTMVDRITP TE  H +++ +D+ V+D WPV+AE + QW+VED F +G P W+ VG + V DVKPYE+MKLRLLNG HS LSY+S L G+ +VDDA+ N  I  F+  +  E+S  +  VPG+D + Y+K ++ RF NPY+KDK+ RL  DGS+K+ NT+++A+    E S+  L T  + +A AA+IRY+ G + + GE I  + DP A+ELR+ A  A           DG  D GEE +         P+  I  VFG EI + K FVG +K+ L SI   G + TL
Sbjct:   27 DPLVLRKKAADFRKGYGNWRSKKSKGARGETNYPTDKDKLHATDAKHEELVLDVVPLTRVNSVEMLKA----------------PDTPGSRGVSRRGSLFRCNSIIDGNDLEAHQEKENWLKFYVNPYSGMAEAELTEPVALNQANLEKVIPATVVKPQYARPGDGRFIDHY--MCHMGVGGFHRSHQAVYTDDLLNLAASLEAKNG------SPSDVKKWGIVGIGLMPWDSKMYNILKAQDHLYTLLSRGHTGSEARVVGSIVDFMFAPENHQAVIDKLAHVDTRIVSLTMTEKGYCQDVNGDLDRANALVKSDLDGDLAKPSSAIGMIVAALRKRKQEGTPSFTVLSCDNLPENGDKIKHVVLQMAGY-VSAELREWVESNTTFPNTMVDRITPMTEVEHIELVARDYMVKDSWPVIAEDFSQWVVEDNFCNGMPEWDKVGALVVKDVKPYEFMKLRLLNGGHSCLSYISVLCGYNYVDDAMANPLISGFIFQFFNEMSPTLLPVPGVDTLTYQKKLVERFGNPYIKDKLTRLAKDGSKKMANTLKEAV---IELSEKGLSTKVIALANAAWIRYMVGRMPETGEAILGIKDPAADELRDLAVEAL----------DG-VDYGEEPK---------PERYIEFVFGAEIVELKGFVGEIKDLLRSIVTHGALTTL 656          

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|298706007|emb|CBJ29121.1| (Mannitol dehydrogenase rossman domain family [Ectocarpus siliculosus])

HSP 1 Score: 523.087 bits (1346), Expect = 3.855e-174
Identity = 295/681 (43.32%), Postives = 412/681 (60.50%), Query Frame = 0

Query:   52 DPHELQRKANKFRQGYKNWRQKKPFGARGEAKDLKAQREEEAAEEKEEEPAPKLQHGRTISSMEPTASGVEASVGSGTGHEVIIPLSPVKGLARRKSFSVSLSAQDLT----AEAEENWLKFYVNPFAELLDKKVG-PLPLCTKNLETILHRNFKSVRGKGPSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPD-DRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQKVDGNLNTEDPNVKHDL-SDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYMEEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEE--IEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSSGSESYDPKEVISIVFGPEIAKNKAFVGTVKEALESICKRGIMYTLLHV 723
            DP  L++KA  FR+GY NWR KK  GARGE   L          +KE+  A  + H   +    P            +  ++  P +P +GL+RR S     S  D       + +ENWLKFYVNP++ + + ++  P+ L   NLE ++  +    + + P   R    Y  + H+GVGGFHRSHQAV+TDDLL L    +A NG       P D  +W I GIGLM WD KMY  L  QDH+YT+L R   G++AR+VGSI DF+FAP+++QA+   L D  TRIVSLT+TEKGYCQ V+G+L+  +  VK DL  DL+ P +A+GM+VAAL QRR  G  SFTVLSCDN P+NG +++ +V +MA   V  +L  W+  +TTFPNTMVDRITP TEA H +++ +D+ V+D WPV+AE + QW++ED F DG P W+ VG + V DVKPYE+MKLRLLNG HS LSY+S L G+ +VDDA+ + QI  F+  + +E++  +  VPG+D   Y+  ++ RF NPY+KDK+ RL  DGS+K+ NT+++A+  L E     L T  + +A AA+IRY+TG      +  I+ + DP+A EL++ A  A                    N V +G+E   P++ I +VFG EI + + FV  +K+ L+SI  +G   TL  V
Sbjct:   27 DPLVLRKKAADFRKGYGNWRSKKSKGARGETGYLL---------DKEKLHATDVHHTDIVMDAVPLTR-------VNSTEQLKQPDTPGRGLSRRGSLFRCNSIIDGNELDDGKEKENWLKFYVNPYSGMAEAELTEPVALNQGNLEKVIPASVVKPKYQRPGDGRFIDHY--MCHMGVGGFHRSHQAVYTDDLLNLAASLEAENG------APTDVKKWGIVGIGLMPWDSKMYNILKEQDHLYTLLSRGHTGSEARVVGSIVDFMFAPENHQAVIDKLSDAETRIVSLTVTEKGYCQDVNGDLDRTNALVKSDLDGDLAKPSSAIGMIVAALRQRRNNGTPSFTVLSCDNLPENGDKIKHVVLQMAGY-VSPELREWVEANTTFPNTMVDRITPMTEAEHIELVARDYMVKDSWPVIAEDFSQWVIEDNFCDGMPAWDKVGGLVVKDVKPYEFMKLRLLNGGHSCLSYISVLCGYSYVDDAMADPQISGFISAFFQEIAPTLLPVPGVDTKTYQAKLVERFGNPYIKDKLTRLAEDGSKKMPNTLKEAVMELREKG---LSTKVIALANAAWIRYMTGKGPIWGDVIIQGIKDPLAAELQDLAEEAI-------------------NGVDTGAEP-KPEKYIELVFGQEIVELEGFVEEIKDCLKSIVNKGAPVTLEEV 659          

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|873238075|emb|CEL96164.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 426.787 bits (1096), Expect = 8.077e-136
Identity = 260/671 (38.75%), Postives = 374/671 (55.74%), Query Frame = 0

Query:    7 IPELPKREGVEPEWNMDLDALDLHIPQPVINIEIEANGAITLTKTDPHELQRKANKFRQGYKNWRQKKPFGARGEAKDL--KAQREEEAAEEKEEEPAPKLQHGRTISSMEPTASGVEASVGSGTGHEVIIPLSPVKGLARRKSFSVSLSAQDLTAEAEENWLKFYVNPFAELLD-----KKVGPLPLCTKNLETI--LHRNFKSVRGKGPSYDRSPGPY-SRIAHVGVGGFHRSHQAVFTDDLL--------ELHKDKQAGNGKGGGKAG--------PPDDRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQKVDGNLNTEDPNVKHDLSDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMA-HLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYMEEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELREPARLA 650
            +P+  + E + PE N +  AL+L +P     + +     I         ++ K N+FR+GY+NWR     GARGEAKDL  + QR+ +A  +   +   +                  A+ G G       P +P         F+            EE+WL+FY +P   LL      KK   LP+C  NL  I  L    +    K P Y+R        I H+GVGGFHRSHQAV+ D+LL         LH    + + K GG  G          D +W +CG+GLM +D KM +AL  QD++YTVL RS   N AR+VGS+ D+V+ PD  +     + DP T+IVSLT+TEKGYCQ  DG L+ ++P ++HDL DLS P +A+G + A L +RR A +  FTV++CDN P+NG    ++V + A        L  W+ ++  FP++MVDRITP T   H+DIL QDF + DEWPVVAE + QW++EDKF  GRPR+E VG MFV DV  YE MKLRLLNG H  L+Y+ +L G++FVDDA+ +  +  F+  YM+EV+  V  V  +++ +YK+ ++ RF N ++KDK+ R+  DGS K  NTMR+++    E  D  L   +  + +A ++RY+   D++G+ IE + DP +  L + A+ A
Sbjct:   17 LPDRRESEEITPESN-EFAALNLILPDEAAPLSLPGYETIA-------RIREKTNEFRKGYQNWRLHGAKGARGEAKDLFERKQRKGDATAKTRVDFQRRASREDFADEQLIDDKDAAAAGGGGAAAVGAAPTTP-------HHFA-----------NEEDWLRFYFSPHTPLLSLDEARKKSLLLPICQANLPRIKKLSEANRWHELKIPGYNRDASTVDCWIMHIGVGGFHRSHQAVYLDELLTAQARARESLHATGGSSSPKAGGGGGGGESAESLAKDPKWGLCGVGLMPFDTKMRDALRNQDYLYTVLTRSSTSNAARVVGSMMDYVYVPDQPERAIQRVSDPRTKIVSLTVTEKGYCQATDGGLDFQNPLIQHDLKDLSCPSSAIGFITAGLIRRRDANQVPFTVMACDNLPENGIVARRMVLDFAGRYDPSGALKEWVEENVCFPSSMVDRITPQTGPEHRDILAQDFLLLDEWPVVAEEFRQWVIEDKFSCGRPRFEHVGAMFVKDVHSYELMKLRLLNGGHGVLAYLGYLLGYRFVDDAVADVLVSSFLRSYMDEVTPTVKNV-RIEIERYKEKIVERFGNRFIKDKIARIAEDGSTKFYNTMRESV---MELCDRGLHIHHCSLGLAGWMRYMAAEDEQGDPIE-LKDPKSGALSKLAKDA 656          

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|551581251|ref|XP_005776122.1| (hypothetical protein EMIHUDRAFT_469450 [Emiliania huxleyi CCMP1516] >gi|485628191|gb|EOD23693.1| hypothetical protein EMIHUDRAFT_469450 [Emiliania huxleyi CCMP1516])

HSP 1 Score: 401.364 bits (1030), Expect = 3.956e-124
Identity = 241/600 (40.17%), Postives = 324/600 (54.00%), Query Frame = 0

Query:  161 EAEENWLKFYVNPFAELLDKKVGPLPLCTKNLETILHRNFKSVRGKGPSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPDDRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQKVDGNLNTEDP---NVKHDLSDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWE-----DVG------VMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYMEEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAI------------------------KFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSSGSESYDPKEVISIVFGPEIAKNKAFVGTVKEALESICKRGIMYTLLH 722
            EA  +WL+F  NPF+E    K   LPLC  NL  I      +      S + +    + IAH+GVGGFHRSH A  TD LL     + AG  K         +RW I G+GLM WDKKM E L  QD +Y++L R      AR++GSI +FVF P D +A  + L   + RIVSLT+TEKGY +  DG+L+   P   +       L+ PRTA G++   L +RR AG    TVLSCDN P NG     +  + A  AV  DL +W+     FPN+MVDRITPATE  H+++L+ ++G+ DEWPV+AE + QW++ED F  GRP WE     DV       VM   DV+PYE MKLRLLN +HSALSY + L    FVDDAL +  +  F+  YM EV   +  V G+DL +Y++ +L RFSN Y+KDK+ RL LDGSQK  NT++ A+                        K   EG+D       + +A+AA++RY  G D  GE +E V DP+ + LR  A  A     L   P  G+  + E   + +       +  +  +FG E+A  +AFV  V E  + I +R  M  +L 
Sbjct:  242 EASGDWLRFAFNPFSEPAAPKRQALPLCAANLAAIGAAGSAASLPAQGSREAT----AHIAHIGVGGFHRSHLAYVTDVLL---TKQHAGEVKAA-------ERWGIIGVGLMPWDKKMAEVLTKQDGLYSLLLRGNAAASARVIGSILEFVFVPADPEAALARLCSASVRIVSLTVTEKGYYRCTDGSLDRTAPLDIDEWGGARGLAQPRTAFGLICTILQRRRSAGLGPLTVLSCDNMPMNGSVCRAVTLDFAS-AVDPDLAAWMERDVAFPNSMVDRITPATENEHRELLRSEYGIVDEWPVIAEPFLQWVIEDSFSAGRPSWELAMPNDVWGRPRAEVMLTADVEPYELMKLRLLNSSHSALSYAAMLCDHVFVDDALADPDVFAFLRAYMLEVVKTLGSVHGVDLAEYQRSLLERFSNAYIKDKLARLALDGSQKFMNTLQHALLYYRAQQELVCEVLAKSSWVAPAHKHAKEGTD------IVALALAAFMRYAAGTDASGEPLELV-DPLTDALRPIAVRAYA--GLGGGPCIGDPAAAERGRLEA------TRAFVETIFGAEVAGWEAFVSNVFEHADRI-RRSTMRDVLR 810          

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|915011298|ref|XP_013251381.1| (Mannitol-1-phosphate dehydrogenase, related [Eimeria acervulina] >gi|557124191|emb|CDI78394.1| Mannitol-1-phosphate dehydrogenase, related [Eimeria acervulina])

HSP 1 Score: 391.734 bits (1005), Expect = 2.330e-121
Identity = 234/615 (38.05%), Postives = 345/615 (56.10%), Query Frame = 0

Query:   56 LQRKANKFRQGYKNWRQKKPFGARGEAKDLKAQREEEAAEEKEEEPAPKLQHGRTISSMEPTASGVEASVGSGTGHEVIIPL-SPVKGLARRKSFSVSLSAQDLTA------EAEENWLKFYVNPFAELLDKKVGPLPLCTKNLETILHRNFKSVRGKGPSYD-RSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPDDR--------WAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQKVDGNLNTEDPNVKHDLSDL----------STPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYMEEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELR 644
            ++ +A++FR+GY NWR+ +  GARG+  D+  Q  EEA    ++     +       S   +A  +  S       E +  L   +K        + + + Q + A      + +  WL+F+ NP   LL   V PL          LHR   S R   P Y   S      +AHVGVGGFHRSHQ VF D+LL                AG PDD         WA+CGIG++  DK+M   LH Q+ +YTVL RSQ+  +AR++G++ DFV+AP++   L   LLD  T+++SLTITEKGYC   +G+L+     VK DL+ +           TP+TALG++   L  RR A  + FTVLSCDN P+NG  L+K+V + A   V  ++ +WI  H  FP TMVDRITP T   H  +L++D+G+ D+WPVVAE + QW++ D F + RP +E VG + V +V+ YE MKL+LLNG HS ++YV+ L G++FVD+AL + ++  F+  +M+EV+  + ++  +D+ +YK+ ++ RF N +VKD+++R+  DGS K  +T R++I  L E     L    L IA+AA+I Y      +G  I    DP ++ELR
Sbjct:  119 MRARAHEFRKGYHNWRRHQAHGARGDVHDVFTQ--EEAPYLTQQSSTNGIADWSVGGSSLESAGILMYSSKPNESSEFLSSLVCSLKTPGAAPPSAAAEALQLMHAAGRAAEQQQPQWLRFFFNPHTPLLSNGVLPLR------RDFLHR--ISERAAVPQYPLESSAIQCWVAHVGVGGFHRSHQCVFYDELL------------AKTHAGLPDDLPEGVHKGPWAVCGIGILPGDKRMSNVLHEQEFLYTVLTRSQRVCEARVIGALMDFVYAPEEPLRLKGLLLDIRTKLLSLTITEKGYCMATNGDLDKSLAAVKQDLALMKCKDIRKQESCTPQTALGLIYLGLKLRRDAKIRPFTVLSCDNIPNNGKLLKKMVIQFA-TEVDVEMATWISKHVCFPCTMVDRITPVTGTEHIALLEEDYGIGDKWPVVAEDFKQWVIGDNFCNERPFFEAVGCLVVREVQSYEEMKLKLLNGGHSCVAYVATLLGYRFVDEALRDVRVSGFLRSFMDEVTPCLEDLK-VDVERYKERIVERFGNAFVKDELQRVAQDGSSKFYSTTRNSILLLLERKKNCLK---LSIALAAWILYFATDSVQGTPIVPS-DPKSKELR 705          

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|1125275512|gb|OLC20279.1| (mannitol dehydrogenase [Deltaproteobacteria bacterium 13_1_40CM_68_24] >gi|1125414976|gb|OLD47060.1| mannitol dehydrogenase [Chloroflexi bacterium 13_1_40CM_2_68_14])

HSP 1 Score: 380.948 bits (977), Expect = 3.215e-121
Identity = 209/529 (39.51%), Postives = 305/529 (57.66%), Query Frame = 0

Query:  199 NFKSVRGKG--PSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPDDRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGY-CQKVDGNLNTEDPNVKHDLSDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYM-EEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKL-RNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSSGSESYDPKEVISI--VFGPEIAKNKAFVGTVKEALESICKRGIMYTL 720
            N KS+ G+   PS+D +  P S I HVGVGGFHR+HQAV+ DDLL           +GG +      +W +CG+GL+  D +M + L  QD +YTV+ RS  G+ AR++GSI  F+FAPD+ +A+   +  P  RIVSLTITE GY   +  G  ++  P+++HDL     P+ A G L  ALD+RR+ G   FTV+SCDN   NG    ++    A      DL  WI ++  FPN MVDRITPAT   H+D++++ FG+ D WPVV E + QW++ED+F  GRP+WE VG   V DV PYE MK+RLLN +H AL Y+  L G+++ D+ + +  IR  VE  M +EV+  +P VPG+DL QY++ ++ RF NP ++D++ R+  +GS ++ +  +   ++ ++ G     P   L   VAA+ RY+ G D+ G  +  + DP++EEL   AR                          +G E  DPKE++ I  +FG  + K  AF   + EAL+S+ + G    L
Sbjct:   11 NLKSIAGRAQVPSFDPAGVPGS-IVHVGVGGFHRAHQAVYLDDLL----------AQGGAR------QWGLCGVGLLPHDARMRDVLRAQDCLYTVIERSGAGDRARVIGSIGSFLFAPDEPEAVIEKMASPGCRIVSLTITEGGYYVNEGTGQFDSAHPDIRHDLEHPHAPKGAFGYLAEALDRRRQRGLAPFTVMSCDNLQGNGDIARRMFLAFAERR-DEDLAKWIAENGAFPNGMVDRITPATTDAHRDLVRESFGIIDGWPVVTEPFKQWVIEDRFPLGRPQWERVGAQMVSDVHPYEMMKIRLLNASHQALCYIGMLLGYRYADETMADADIRALVERMMNDEVTPLLPPVPGIDLAQYRRTLIERFGNPVLRDQLARIGTEGSARIPKFVLPSILERVSRGG----PMRALTFTVAAWFRYLVGSDERGNPL-PINDPMSEELTRRAR--------------------------AGGE--DPKELLGIEKLFGSVLPKAPAFTNYLAEALKSLHREGARQAL 488          

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|1125337458|gb|OLC78174.1| (mannitol dehydrogenase [Deltaproteobacteria bacterium 13_1_40CM_4_68_19])

HSP 1 Score: 378.637 bits (971), Expect = 2.689e-120
Identity = 209/529 (39.51%), Postives = 304/529 (57.47%), Query Frame = 0

Query:  199 NFKSVRGKG--PSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPDDRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARIVGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGY-CQKVDGNLNTEDPNVKHDLSDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYM-EEVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKL-RNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSSGSESYDPKEVISI--VFGPEIAKNKAFVGTVKEALESICKRGIMYTL 720
            N KS+ G+   PS+D +  P S I HVGVGGFHR+HQAV+ DDLL           +GG +      +W +CG+GL+  D +M + L  QD +YTV+ RS  G+ AR++GSI  F+FAPD+ +A+   +  P  RIVSLTITE GY   +  G  ++  P+++HDL     P+ A G L  ALD+RR+ G   FTV+SCDN   NG    ++    A      DL  WI ++  FPN MVDRITPAT   H+D++++ FG+ D WPVV E + QW++ED+F  GRP+WE VG   V DV PYE MK+RLLN +H AL Y+  L G+++ D+ + +  IR  VE  M +EV+  +P VPG+DL QY++ ++ RF NP ++D++ R+  +GS ++ +  +   ++ ++ G     P   L   VAA+ RY+ G D+ G  +  + DP++EEL   AR                          +G E  DPKE++ I  +FG  + K  AF   + EAL+S+   G    L
Sbjct:   11 NLKSIAGRAQVPSFDPAGVPGS-IVHVGVGGFHRAHQAVYLDDLL----------AQGGAR------QWGLCGVGLLPHDARMRDVLRAQDCLYTVIERSGAGDRARVIGSIGSFLFAPDEPEAVIEKMASPGCRIVSLTITEGGYYVNEGTGQFDSAHPDIRHDLEHPHAPKGAFGYLAEALDRRRQRGLAPFTVMSCDNLQGNGDIARRMFLAFAERR-DEDLAKWIAENGAFPNGMVDRITPATTDAHRDLVRESFGIIDGWPVVTEPFKQWVIEDRFPLGRPQWERVGAQMVSDVHPYEMMKIRLLNASHQALCYIGMLLGYRYADETMADADIRALVERMMNDEVTPLLPPVPGIDLAQYRRTLIERFGNPVLRDQLARIGTEGSARIPKFVLPSILERVSRGG----PMRALTFTVAAWFRYLVGSDERGNPL-PINDPMSEELVRRAR--------------------------AGGE--DPKELLGIEKLFGSVLPKAPAFTNYLAEALKSLHLEGARQAL 488          

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|1001743060|ref|WP_061291853.1| (mannitol dehydrogenase, partial [Azotobacter vinelandii])

HSP 1 Score: 374.015 bits (959), Expect = 1.586e-118
Identity = 222/519 (42.77%), Postives = 294/519 (56.65%), Query Frame = 0

Query:  208 PSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPDDRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARI--VGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQK-VDGNLNTEDPNVKHDLSDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYME-EVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSS--GSESYDPKEVISIVFGPEIAKNKAFVGTVKEALESICKRGIMYTL 720
            P+YDR       I H+GVGGFHR+HQAV+T+ LL              GKAG     W ICG+GL   D+ M EAL  QD++YT L+    G+D +I  VG+I DF+ A DD +AL   L DPATRIVSLTITE GYC     G  N + P ++HDL++   PRT  G L  AL +RR AG + FTV+SCDN P NG      +   A L   + L  WI D  +FPN MVDRITP T   H+  L+++ G+ED WPVVAE + QW+VED F +GRP WE+VGV F  DV PYE MK+RLLNG+H A++Y+  L G +   +A+ + Q+RD+V  YM+ +V+  + EVPG+DL +YK  ++ RFSN  + D++ RL  DGS K    +   +  L E  +   P     + VAA+  Y+ GID+ GE    + DP A  LRE    A C+               EE  V +  G E          VFG EI  ++AFV   +  LE +   G+  TL
Sbjct:   18 PAYDRGQIGQG-IVHIGVGGFHRAHQAVYTEMLLNR------------GKAG----EWGICGVGLRPEDRGMQEALASQDYLYT-LYELGDGSDTQIQVVGAIVDFLLADDDAEALIRKLADPATRIVSLTITEGGYCTDDSTGQFNADLPQIRHDLANPQRPRTVFGFLSEALARRRAAGIEPFTVMSCDNLPHNGRVARNALLGFARLR-DAGLHDWIADKVSFPNAMVDRITPMTSDAHRARLREETGIEDAWPVVAEPFLQWVVEDDFCNGRPAWEEVGVQFTEDVTPYEAMKIRLLNGSHLAMAYLGALLGHRHAHEAMQDGQLRDYVRAYMDRDVTPLLAEVPGIDLAEYKDTLIERFSNTAICDQISRLCSDGSSKFPKFV---LPTLLEQIEAGAPLQRTALIVAAWCHYLRGIDERGETY-PIPDPRAAWLRE----AACV---------------EERLVETFLGLEE---------VFGHEIPASEAFVAAFRLQLERLRSLGVRATL 485          

BLAST of EWM26995.1 vs. NCBI_GenBank
Match: gi|502033290|ref|WP_012701298.1| (mannitol 2-dehydrogenase [Azotobacter vinelandii] >gi|226719739|gb|ACO78910.1| Mannitol dehydrogenase [Azotobacter vinelandii DJ] >gi|482530617|gb|AGK13744.1| Mannitol dehydrogenase [Azotobacter vinelandii CA] >gi|482535203|gb|AGK18329.1| Mannitol dehydrogenase [Azotobacter vinelandii CA6] >gi|1104086183|emb|SFY29258.1| mannitol 2-dehydrogenase [Azotobacter vinelandii])

HSP 1 Score: 371.318 bits (952), Expect = 1.691e-117
Identity = 222/519 (42.77%), Postives = 293/519 (56.45%), Query Frame = 0

Query:  208 PSYDRSPGPYSRIAHVGVGGFHRSHQAVFTDDLLELHKDKQAGNGKGGGKAGPPDDRWAICGIGLMTWDKKMYEALHGQDHMYTVLFRSQKGNDARI--VGSIFDFVFAPDDYQALFSTLLDPATRIVSLTITEKGYCQK-VDGNLNTEDPNVKHDLSDLSTPRTALGMLVAALDQRRKAGRKSFTVLSCDNQPDNGHQLEKLVKEMAHLAVGSDLTSWIGDHTTFPNTMVDRITPATEAVHKDILKQDFGVEDEWPVVAEHYGQWIVEDKFVDGRPRWEDVGVMFVPDVKPYEYMKLRLLNGTHSALSYVSHLSGFQFVDDALNNEQIRDFVECYME-EVSAAVPEVPGLDLVQYKKDVLTRFSNPYVKDKVERLMLDGSQKLRNTMRDAIKFLTEGSDPPLPTTYLGIAVAAYIRYVTGIDQEGEEIEKVLDPIAEELREPARLACCLRSLPPSPTDGESDSGEENEVSS--GSESYDPKEVISIVFGPEIAKNKAFVGTVKEALESICKRGIMYTL 720
            P+YDR       I H+GVGGFHR+HQAV+T+ LL              GKAG     W ICG+GL   D+ M EAL  QD++YT L+    G+D +I  VG+I DF+ A DD +AL   L DPATRIVSLTITE GYC     G  N + P ++HDL++   PRT  G L  AL +RR AG + FTV+SCDN P NG      +   A L   + L  WI    +FPN MVDRITP T   H+  L+++ G+ED WPVVAE + QW+VED F +GRP WE+VGV F  DV PYE MK+RLLNG+H A++Y+  L G +   +A+ + Q+RD+V  YM+ +V+  + EVPG+DL +YK  ++ RFSN  + D+V RL  DGS K    +   +  L E  +   P     + VAA+  Y+ GID+ GE    + DP A  LRE    A C+               EE  V +  G E          VFG EI  ++AFV   +  LE +   G+  TL
Sbjct:   18 PAYDRGQIGQG-IVHIGVGGFHRAHQAVYTEMLLNR------------GKAG----EWGICGVGLRPEDRGMQEALASQDYLYT-LYELGDGSDTQIQVVGAIVDFLLADDDAEALIRKLADPATRIVSLTITEGGYCTDDSTGQFNADLPQIRHDLANPQRPRTVFGFLSEALARRRAAGIEPFTVMSCDNLPHNGRVARNALLGFARLR-DAGLHDWIAGKVSFPNAMVDRITPMTSDAHRVRLREETGIEDAWPVVAEPFLQWVVEDDFCNGRPAWEEVGVQFTEDVTPYEAMKIRLLNGSHLAMAYLGALLGHRHAHEAMQDGQLRDYVRAYMDRDVTPLLAEVPGIDLAEYKDTLIERFSNTAICDQVSRLCSDGSSKFPKFV---LPTLLEQIEAGAPLQRTALIVAAWCHYLRGIDERGETY-PIPDPRAAWLRE----AACV---------------EERLVETFLGLEE---------VFGHEIPASEAFVAAFRLQLERLRSLGVRATL 485