EWM26996.1, cds3668 (CDS) Nannochloropsis gaditana

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|585109166|gb|EWM26996.1| (snf2 histone linker phd ring helicase [Nannochloropsis gaditana])

HSP 1 Score: 4814.21 bits (12486), Expect = 0.000e+0
Identity = 2343/2343 (100.00%), Postives = 2343/2343 (100.00%), Query Frame = 0

Query:    1 MGRTKARPRRVGSLFEHNEWVENRERQGLSRLDAALQEANEANNDFLPRSAFAPCRGGAASGDVAVAPDAGRRRSGQWKGKVNHRDIEGMLNDYDYNMATGEDAEEGGDEDVEKRREEEEREEADGMEEVYEEAAAARLGHRHPRRYRSAMVTTKVSADQAERLQALDGREETRKRRRLYREGKRVPMPTQEDQECSPLEVSDEFQSHIRLALPVPAGEKELRALLVRRRSFSQAAAQDCRGRPLATVLIPATVSARRTLRVSLAENMATLVLEGDAPQLLGVKDCESWDLLQSLCEATPPFAYYEAISDGRAVHLDIYLTARTLALASPSNEPPVKSVHLKRLRRLLELFFPAVLPELSTYYKPFPITLGTPAEPGGAGKGVIEEEESSKRAVPFDPADLYRCLEEYKAARLVQRERLPCLVQQSQASPNQGQAREEEPSVPGLKVTLRPYQARAVEWMQCREQARDYFAPRAPSPSGSHAENPEDDMDEENLLGHGWIKLLLPNPVPDPSASFVYYNPISVSLSHRWPVMWEPRALKGGILADEMGLGKTIEVMALVLSHRYLGGEKAAKEAKGSSLGCHEKRGRGNPEKGGNCAPMHNGSPNKEAISLDEDRIVKEGDGNDGGGGNPGREGSGGKDRKRGHDERRHRGRAEEGLVDRTEYVCPCGRDRRGEAWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGELWERERGKQRDESEKDTKGKGTPAKASTSPSAQDVREEGNARESDGRSKAHCTKKAGQEHGGISGKLKFLMEAASYYEKALLTAERNRQPVPIGGVEGLEGAPRFKGRESRDVDAGNDGRMEGRKKQWTVINLEWEMKQELKEGNTVEEGVLDVDAVRQKISQNEDLHPASTWTATELSPLWASVTFQGGNKKIMGLRVWPLVNSEDSGGEEAQTKVEDLVYMPGKCRLMACCSPEGALFVEAGVFDLKELMREEGDKGTGKEGKDGGMGGKLILGFNMLAAKTWKLEVLDVIRSEGRREEGKEGGIIKTGMKVQLLVADIEVDPLQILHVTTNLHRVFKEIKGIAGGEVDSGSHSAKIVSSLLSPPNSPSPANEGVTASTQAKHEQESERLMSTDVGVMVRRYEDVYQLHIAEKIEWLDARVRAVTQRECNLARLMHQANRQHFASASQALDALDGEIDKLRKRGGKAAGQGRIVWWEWTLNELMVRGDLLDPKADLLRRLKVESSGPQFPAFHDYHGLMMAIKLKSKDLDETRTRVNALLRRVLVDNPSEGERQRNSDCGVCRKDWGKSGPLCHICELEKHLKAYEEKLHAYREQRQQNGHTVGTFKHSCCTHRLLYSVLLPWLRHQALKRKSNPEWQHAWEEAMVDNRKEELLERERKMAKRFWNSGFDLLSKLDEVACSALSLELAQEGEIFKDMSSEQRKLVVFPHEVDVKKEEYRMQVAKYEVELRERKGYLKYLQNIAKNEARAAERNAAMARKMQKEVKSSASSERKDGAFREGPRIPTSIDPCASSGILQGNDEEGEEDNVNLCFVCFEEYTVINPREVLPCAHHLCKTCLRQLLGKRAQNKCPKCRRNFHRKACAEVQESREGECKDMEEDRKDIRVGEDELYMDEAVQEEGDEEEEEEEEEDDEGPRVEGSWGTKITALVSEVLRLPVEDKCLIFSEWDEMLDIVQQALQTNRIDIVRLKGERTAGRVRQEFRENESVRCMLLNLKSGAKGLTLVEANHVFLLEPIFNAATEAQAVNRVFRIGQTKPTVIHRFVVRNTIEENVEKMRQSKVAAAGGESAVLSAVKKQGKTRKTDVELDVEEMEKLFATTPRVIRRSIMSSPDIASGATVDRMASSVGGGHRNGRRPWQRKINMDRAGEGSSLSNLVNVRRYDGGDGEGMATEEEDGTSGAVECATSDSSGQDGAVRAVQAGTVQSGGENRNVDPHTEMIVDNNNFEEREVTRGGRRLGGAAVAGRGRGCSRSVLLRQSDGI 2343
            MGRTKARPRRVGSLFEHNEWVENRERQGLSRLDAALQEANEANNDFLPRSAFAPCRGGAASGDVAVAPDAGRRRSGQWKGKVNHRDIEGMLNDYDYNMATGEDAEEGGDEDVEKRREEEEREEADGMEEVYEEAAAARLGHRHPRRYRSAMVTTKVSADQAERLQALDGREETRKRRRLYREGKRVPMPTQEDQECSPLEVSDEFQSHIRLALPVPAGEKELRALLVRRRSFSQAAAQDCRGRPLATVLIPATVSARRTLRVSLAENMATLVLEGDAPQLLGVKDCESWDLLQSLCEATPPFAYYEAISDGRAVHLDIYLTARTLALASPSNEPPVKSVHLKRLRRLLELFFPAVLPELSTYYKPFPITLGTPAEPGGAGKGVIEEEESSKRAVPFDPADLYRCLEEYKAARLVQRERLPCLVQQSQASPNQGQAREEEPSVPGLKVTLRPYQARAVEWMQCREQARDYFAPRAPSPSGSHAENPEDDMDEENLLGHGWIKLLLPNPVPDPSASFVYYNPISVSLSHRWPVMWEPRALKGGILADEMGLGKTIEVMALVLSHRYLGGEKAAKEAKGSSLGCHEKRGRGNPEKGGNCAPMHNGSPNKEAISLDEDRIVKEGDGNDGGGGNPGREGSGGKDRKRGHDERRHRGRAEEGLVDRTEYVCPCGRDRRGEAWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGELWERERGKQRDESEKDTKGKGTPAKASTSPSAQDVREEGNARESDGRSKAHCTKKAGQEHGGISGKLKFLMEAASYYEKALLTAERNRQPVPIGGVEGLEGAPRFKGRESRDVDAGNDGRMEGRKKQWTVINLEWEMKQELKEGNTVEEGVLDVDAVRQKISQNEDLHPASTWTATELSPLWASVTFQGGNKKIMGLRVWPLVNSEDSGGEEAQTKVEDLVYMPGKCRLMACCSPEGALFVEAGVFDLKELMREEGDKGTGKEGKDGGMGGKLILGFNMLAAKTWKLEVLDVIRSEGRREEGKEGGIIKTGMKVQLLVADIEVDPLQILHVTTNLHRVFKEIKGIAGGEVDSGSHSAKIVSSLLSPPNSPSPANEGVTASTQAKHEQESERLMSTDVGVMVRRYEDVYQLHIAEKIEWLDARVRAVTQRECNLARLMHQANRQHFASASQALDALDGEIDKLRKRGGKAAGQGRIVWWEWTLNELMVRGDLLDPKADLLRRLKVESSGPQFPAFHDYHGLMMAIKLKSKDLDETRTRVNALLRRVLVDNPSEGERQRNSDCGVCRKDWGKSGPLCHICELEKHLKAYEEKLHAYREQRQQNGHTVGTFKHSCCTHRLLYSVLLPWLRHQALKRKSNPEWQHAWEEAMVDNRKEELLERERKMAKRFWNSGFDLLSKLDEVACSALSLELAQEGEIFKDMSSEQRKLVVFPHEVDVKKEEYRMQVAKYEVELRERKGYLKYLQNIAKNEARAAERNAAMARKMQKEVKSSASSERKDGAFREGPRIPTSIDPCASSGILQGNDEEGEEDNVNLCFVCFEEYTVINPREVLPCAHHLCKTCLRQLLGKRAQNKCPKCRRNFHRKACAEVQESREGECKDMEEDRKDIRVGEDELYMDEAVQEEGDEEEEEEEEEDDEGPRVEGSWGTKITALVSEVLRLPVEDKCLIFSEWDEMLDIVQQALQTNRIDIVRLKGERTAGRVRQEFRENESVRCMLLNLKSGAKGLTLVEANHVFLLEPIFNAATEAQAVNRVFRIGQTKPTVIHRFVVRNTIEENVEKMRQSKVAAAGGESAVLSAVKKQGKTRKTDVELDVEEMEKLFATTPRVIRRSIMSSPDIASGATVDRMASSVGGGHRNGRRPWQRKINMDRAGEGSSLSNLVNVRRYDGGDGEGMATEEEDGTSGAVECATSDSSGQDGAVRAVQAGTVQSGGENRNVDPHTEMIVDNNNFEEREVTRGGRRLGGAAVAGRGRGCSRSVLLRQSDGI
Sbjct:    1 MGRTKARPRRVGSLFEHNEWVENRERQGLSRLDAALQEANEANNDFLPRSAFAPCRGGAASGDVAVAPDAGRRRSGQWKGKVNHRDIEGMLNDYDYNMATGEDAEEGGDEDVEKRREEEEREEADGMEEVYEEAAAARLGHRHPRRYRSAMVTTKVSADQAERLQALDGREETRKRRRLYREGKRVPMPTQEDQECSPLEVSDEFQSHIRLALPVPAGEKELRALLVRRRSFSQAAAQDCRGRPLATVLIPATVSARRTLRVSLAENMATLVLEGDAPQLLGVKDCESWDLLQSLCEATPPFAYYEAISDGRAVHLDIYLTARTLALASPSNEPPVKSVHLKRLRRLLELFFPAVLPELSTYYKPFPITLGTPAEPGGAGKGVIEEEESSKRAVPFDPADLYRCLEEYKAARLVQRERLPCLVQQSQASPNQGQAREEEPSVPGLKVTLRPYQARAVEWMQCREQARDYFAPRAPSPSGSHAENPEDDMDEENLLGHGWIKLLLPNPVPDPSASFVYYNPISVSLSHRWPVMWEPRALKGGILADEMGLGKTIEVMALVLSHRYLGGEKAAKEAKGSSLGCHEKRGRGNPEKGGNCAPMHNGSPNKEAISLDEDRIVKEGDGNDGGGGNPGREGSGGKDRKRGHDERRHRGRAEEGLVDRTEYVCPCGRDRRGEAWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGELWERERGKQRDESEKDTKGKGTPAKASTSPSAQDVREEGNARESDGRSKAHCTKKAGQEHGGISGKLKFLMEAASYYEKALLTAERNRQPVPIGGVEGLEGAPRFKGRESRDVDAGNDGRMEGRKKQWTVINLEWEMKQELKEGNTVEEGVLDVDAVRQKISQNEDLHPASTWTATELSPLWASVTFQGGNKKIMGLRVWPLVNSEDSGGEEAQTKVEDLVYMPGKCRLMACCSPEGALFVEAGVFDLKELMREEGDKGTGKEGKDGGMGGKLILGFNMLAAKTWKLEVLDVIRSEGRREEGKEGGIIKTGMKVQLLVADIEVDPLQILHVTTNLHRVFKEIKGIAGGEVDSGSHSAKIVSSLLSPPNSPSPANEGVTASTQAKHEQESERLMSTDVGVMVRRYEDVYQLHIAEKIEWLDARVRAVTQRECNLARLMHQANRQHFASASQALDALDGEIDKLRKRGGKAAGQGRIVWWEWTLNELMVRGDLLDPKADLLRRLKVESSGPQFPAFHDYHGLMMAIKLKSKDLDETRTRVNALLRRVLVDNPSEGERQRNSDCGVCRKDWGKSGPLCHICELEKHLKAYEEKLHAYREQRQQNGHTVGTFKHSCCTHRLLYSVLLPWLRHQALKRKSNPEWQHAWEEAMVDNRKEELLERERKMAKRFWNSGFDLLSKLDEVACSALSLELAQEGEIFKDMSSEQRKLVVFPHEVDVKKEEYRMQVAKYEVELRERKGYLKYLQNIAKNEARAAERNAAMARKMQKEVKSSASSERKDGAFREGPRIPTSIDPCASSGILQGNDEEGEEDNVNLCFVCFEEYTVINPREVLPCAHHLCKTCLRQLLGKRAQNKCPKCRRNFHRKACAEVQESREGECKDMEEDRKDIRVGEDELYMDEAVQEEGDEEEEEEEEEDDEGPRVEGSWGTKITALVSEVLRLPVEDKCLIFSEWDEMLDIVQQALQTNRIDIVRLKGERTAGRVRQEFRENESVRCMLLNLKSGAKGLTLVEANHVFLLEPIFNAATEAQAVNRVFRIGQTKPTVIHRFVVRNTIEENVEKMRQSKVAAAGGESAVLSAVKKQGKTRKTDVELDVEEMEKLFATTPRVIRRSIMSSPDIASGATVDRMASSVGGGHRNGRRPWQRKINMDRAGEGSSLSNLVNVRRYDGGDGEGMATEEEDGTSGAVECATSDSSGQDGAVRAVQAGTVQSGGENRNVDPHTEMIVDNNNFEEREVTRGGRRLGGAAVAGRGRGCSRSVLLRQSDGI 2343          

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|702472391|ref|XP_010031069.1| (PREDICTED: E3 ubiquitin-protein ligase SHPRH [Eucalyptus grandis] >gi|702472395|ref|XP_010031070.1| PREDICTED: E3 ubiquitin-protein ligase SHPRH [Eucalyptus grandis] >gi|629083971|gb|KCW50328.1| hypothetical protein EUGRSUZ_J00103 [Eucalyptus grandis])

HSP 1 Score: 325.865 bits (834), Expect = 2.727e-86
Identity = 235/673 (34.92%), Postives = 320/673 (47.55%), Query Frame = 0

Query:  441 SVPGLKVTLRPYQARAVEWMQCREQARDYFAPRAPSPSGSHAENPEDDMDEENLLGHGWIKLLLPNPVPDPSASFVYYNPISVSLSHRWPVMWEPRALKGGILADEMGLGKTIEVMALVLSHRYLGGEKAAKEAKGSSLGCHEKRGRGNPEKGGNCAPMHNGSPNKEAISLDEDRIVKEGDGNDGGGGNPGREGSGGKDRKRGHDERRHRGRAEEGLVDRTEYVCPCG----RDRRGEAWLQCDACKHWAHVRCAGLSS-------------------SAEAEALPA-------FVCEVCHGLERF-QSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQL-RNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKR--MQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIK 1079
            ++P L   LRPYQ RA  WM   E+             GS        +     +  G++           S   ++YNP S +++ R  V  E   + GGILADEMGLGKT+E++A +LSHR+                        +  K G C        N+E+    E +I                           H +R  R R E          C CG      R    W+QCD C  W H  C G S+                   S   + +P        +VC++C  L    +SP P  ATLIV P  I  QW+ E        S  ++V+ Y GV  TS S    E   ++ D L + YDIVLTT+DVL +DL H DS      R E  R+L R +K+Y V P+PL  + WWR+CLDEAQM+E++AAAA  MALRL + HRWC++GTPI +  LDDLYGLL FL++SPFD   WW   I   +EK D  A    H L   IMWR+SK+ V D+L LPPQ E    L FS +E  +YQ Q++ C+   R++V  IK   ++++     S    ++    + A   L S    LL+LRQACCHPQ+G+ GL  L+     M+M+EVL  L    K+  EE  R  ++ L+GL  +A I+
Sbjct:  284 NIPNLIPKLRPYQRRAAYWMVQNEKG----------ALGSFGTQGTSQLVSPLCMTVGFVD----------SGLKMFYNPFSGNVAMRSEVFSE--RVSGGILADEMGLGKTVELLACILSHRW------------------------SASKSGACI-------NEESQLRKESQI---------------------------HIKRLKRERVE----------CICGAVTESYRYTGLWVQCDVCDAWQHSDCVGYSARGKKVKDGSGQQKREDIEKSRRKKNVPTIVEREGKYVCQLCLELMSVTESPVPSGATLIVCPAPILHQWQTEIIRHTNTDS--VKVLVYEGVKNTSFS----EEPAVEVDELVS-YDIVLTTYDVLKEDLSH-DS-----DRHEGDRRLMRYQKRYPVIPTPLTRIFWWRLCLDEAQMVESNAAAATEMALRLHSKHRWCITGTPIQR-RLDDLYGLLKFLKLSPFDVPRWWNEVIRDPYEKRDAGAMEFTHNLFKQIMWRSSKRHVADELHLPPQEECLSWLSFSAIEEHFYQRQHETCVDYARELVESIKADILKREAPGSASSAPSDILITHVEAAKLLNS----LLKLRQACCHPQVGSSGLRSLQHSP--MSMEEVLTVLISKTKIEGEEALRRSVVALNGLAGIAIIE 846          

HSP 2 Score: 143.665 bits (361), Expect = 2.477e-30
Identity = 102/328 (31.10%), Postives = 166/328 (50.61%), Query Frame = 0

Query: 1884 EEGEEDNVNL----CFVCFEEYTVINPREVLPCAHHLCKTCLRQLL-GKRAQN--------KCPKCRRNFHRKACAEVQESREGECKDMEEDRKDIRVGEDELYMDEAVQEEGDEEEEEEEEEDDEGPRVEGSWGTKITALVSEVLRLPVED---KCLIFSEWDEMLDIVQQALQTNRIDIVRLKGERTAGRVRQEFR-ENESVRC-----------MLLNLKSGAKGLTLVEANHVFLLEPIFNAATEAQAVNRVFRIGQTKPTVIHRFVVRNTIEENVEKMRQSKVAAAGGESAVLSAVKKQGKTRKTDVE-LDVEEMEKLFATTP 2182
            E+  E +VN     C +C E+ ++   + V  C H +C  CL +L   K+AQ          CP CR++      A              +DR++       L+             E ++  ++    V+GS+GTKI A+   +L +   +   K L+FS W ++LD+++ A + N I   R+KG R A     +FR +N SV+            +LL ++ GA GL L+EA HV L+EP+ N A EAQA++RV RIGQ   T++HRF+V+N++EE++ K+ +S+       S+ +S     G T+  D   L ++++E LFA +P
Sbjct: 1342 EQKSEHSVNAYQEACPICQEKLSI--QKMVFQCGHVICCKCLFELSEHKQAQAGESQRRWVMCPTCRQHTEFANIA------------YADDRQNKLPNSSGLH-------------ESQDSRNEASITVQGSYGTKIEAVTRRILWIKSTEPNAKVLVFSSWKDVLDVLEHAFKANSISYARMKGGRKAHTAVDQFRGQNPSVKVNTREHCQDIQVLLLLVQHGANGLNLLEARHVILVEPLLNPAAEAQAISRVHRIGQENKTLVHRFIVKNSVEESIYKLNKSR-----NSSSFIS-----GNTKNQDQPVLTLKDVESLFAASP 1632          

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|629083972|gb|KCW50329.1| (hypothetical protein EUGRSUZ_J00103 [Eucalyptus grandis])

HSP 1 Score: 325.094 bits (832), Expect = 4.078e-86
Identity = 235/673 (34.92%), Postives = 320/673 (47.55%), Query Frame = 0

Query:  441 SVPGLKVTLRPYQARAVEWMQCREQARDYFAPRAPSPSGSHAENPEDDMDEENLLGHGWIKLLLPNPVPDPSASFVYYNPISVSLSHRWPVMWEPRALKGGILADEMGLGKTIEVMALVLSHRYLGGEKAAKEAKGSSLGCHEKRGRGNPEKGGNCAPMHNGSPNKEAISLDEDRIVKEGDGNDGGGGNPGREGSGGKDRKRGHDERRHRGRAEEGLVDRTEYVCPCG----RDRRGEAWLQCDACKHWAHVRCAGLSS-------------------SAEAEALPA-------FVCEVCHGLERF-QSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQL-RNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKR--MQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIK 1079
            ++P L   LRPYQ RA  WM   E+             GS        +     +  G++           S   ++YNP S +++ R  V  E   + GGILADEMGLGKT+E++A +LSHR+                        +  K G C        N+E+    E +I                           H +R  R R E          C CG      R    W+QCD C  W H  C G S+                   S   + +P        +VC++C  L    +SP P  ATLIV P  I  QW+ E        S  ++V+ Y GV  TS S    E   ++ D L + YDIVLTT+DVL +DL H DS      R E  R+L R +K+Y V P+PL  + WWR+CLDEAQM+E++AAAA  MALRL + HRWC++GTPI +  LDDLYGLL FL++SPFD   WW   I   +EK D  A    H L   IMWR+SK+ V D+L LPPQ E    L FS +E  +YQ Q++ C+   R++V  IK   ++++     S    ++    + A   L S    LL+LRQACCHPQ+G+ GL  L+     M+M+EVL  L    K+  EE  R  ++ L+GL  +A I+
Sbjct:  284 NIPNLIPKLRPYQRRAAYWMVQNEKG----------ALGSFGTQGTSQLVSPLCMTVGFVD----------SGLKMFYNPFSGNVAMRSEVFSE--RVSGGILADEMGLGKTVELLACILSHRW------------------------SASKSGACI-------NEESQLRKESQI---------------------------HIKRLKRERVE----------CICGAVTESYRYTGLWVQCDVCDAWQHSDCVGYSARGKKVKDGSGQQKREDIEKSRRKKNVPTIVEREGKYVCQLCLELMSVTESPVPSGATLIVCPAPILHQWQTEIIRHTNTDS--VKVLVYEGVKNTSFS----EEPAVEVDELVS-YDIVLTTYDVLKEDLSH-DS-----DRHEGDRRLMRYQKRYPVIPTPLTRIFWWRLCLDEAQMVESNAAAATEMALRLHSKHRWCITGTPIQR-RLDDLYGLLKFLKLSPFDVPRWWNEVIRDPYEKRDAGAMEFTHNLFKQIMWRSSKRHVADELHLPPQEECLSWLSFSAIEEHFYQRQHETCVDYARELVESIKADILKREAPGSASSAPSDILITHVEAAKLLNS----LLKLRQACCHPQVGSSGLRSLQHSP--MSMEEVLTVLISKTKIEGEEALRRSVVALNGLAGIAIIE 846          

HSP 2 Score: 122.479 bits (306), Expect = 6.788e-24
Identity = 86/272 (31.62%), Postives = 134/272 (49.26%), Query Frame = 0

Query: 1884 EEGEEDNVNL----CFVCFEEYTVINPREVLPCAHHLCKTCLRQLL-GKRAQN--------KCPKCRRNFHRKACAEVQESREGECKDMEEDRKDIRVGEDELYMDEAVQEEGDEEEEEEEEEDDEGPRVEGSWGTKITALVSEVLRLPVED---KCLIFSEWDEMLDIVQQALQTNRIDIVRLKGERTAGRVRQEFR-ENESVRC-----------MLLNLKSGAKGLTLVEANHVFLLEPIFNAATEAQAVNRVFRIGQTKPTVIHRFVV 2127
            E+  E +VN     C +C E+ ++   + V  C H +C  CL +L   K+AQ          CP CR++      A              +DR++       L+             E ++  ++    V+GS+GTKI A+   +L +   +   K L+FS W ++LD+++ A + N I   R+KG R A     +FR +N SV+            +LL ++ GA GL L+EA HV L+EP+ N A EAQA++RV RIGQ   T++HRF+V
Sbjct: 1342 EQKSEHSVNAYQEACPICQEKLSI--QKMVFQCGHVICCKCLFELSEHKQAQAGESQRRWVMCPTCRQHTEFANIA------------YADDRQNKLPNSSGLH-------------ESQDSRNEASITVQGSYGTKIEAVTRRILWIKSTEPNAKVLVFSSWKDVLDVLEHAFKANSISYARMKGGRKAHTAVDQFRGQNPSVKVNTREHCQDIQVLLLLVQHGANGLNLLEARHVILVEPLLNPAAEAQAISRVHRIGQENKTLVHRFIV 1586          

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|1043420973|gb|OCA33603.1| (hypothetical protein XENTR_v90013469mg, partial [Xenopus tropicalis])

HSP 1 Score: 293.508 bits (750), Expect = 3.334e-78
Identity = 170/421 (40.38%), Postives = 238/421 (56.53%), Query Frame = 0

Query:  663 YVCPCGRD--RRGEAWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGE 1081
            + C CG       +A +QC  C  W H  C       E   +  F C  C    +   P P  ATLI++P  IC QW +E    +Q  S+ L V+ Y+GV         ++   L P +L  E D+V+TT+DVL  +L + D   +P S  E GR+ RN+K+Y   PSPL ++ WWRICLDEAQM+E + A AA MALRL  ++RWCVSGTP+ +G L+DL+GL+LFL V P+  + WW   +   + + DP     L++ +S IMWR++KK VLDQ+ +PPQTE    LHFS VER +Y  Q++ C    +D + K +++     + +S     L +R +S      S   PLLRLRQACCHPQ   G    L+K    MTM+E+L  L++  +  CEE  R L+  L+GL  +  IKGE
Sbjct:  621 FECICGELGLYDYKARVQCLQCHLWQHAECVNYKQ--ENLGVTPFYCPHCLVAMK---PVPTGATLIISPSSICHQWVDEINRHVQ--SSSLHVMVYQGV---------KKHGFLQPQMLA-EQDVVITTYDVLRSELNYVD---IPHSNSEDGRKFRNQKRYMAVPSPLVAVEWWRICLDEAQMVECTTAKAAEMALRLSGINRWCVSGTPVQRG-LEDLFGLVLFLGVDPYWVKHWWDQLLYKPYCRKDPFP---LYKFISKIMWRSAKKDVLDQIQIPPQTEDVHWLHFSPVERHFYHRQHEVC---CQDALAKFRKITDWALKLSS-----LDRRTVS------SILYPLLRLRQACCHPQAVRGEFLPLQK--STMTMEELLTSLQKKCRTECEEAHRQLVCALNGLAGIHIIKGE 1001          

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|1062897252|ref|XP_017949475.1| (PREDICTED: E3 ubiquitin-protein ligase SHPRH [Xenopus tropicalis])

HSP 1 Score: 295.434 bits (755), Expect = 7.146e-77
Identity = 170/421 (40.38%), Postives = 238/421 (56.53%), Query Frame = 0

Query:  663 YVCPCGRD--RRGEAWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGE 1081
            + C CG       +A +QC  C  W H  C       E   +  F C  C    +   P P  ATLI++P  IC QW +E    +Q  S+ L V+ Y+GV         ++   L P +L  E D+V+TT+DVL  +L + D   +P S  E GR+ RN+K+Y   PSPL ++ WWRICLDEAQM+E + A AA MALRL  ++RWCVSGTP+ +G L+DL+GL+LFL V P+  + WW   +   + + DP     L++ +S IMWR++KK VLDQ+ +PPQTE    LHFS VER +Y  Q++ C    +D + K +++     + +S     L +R +S      S   PLLRLRQACCHPQ   G    L+K    MTM+E+L  L++  +  CEE  R L+  L+GL  +  IKGE
Sbjct:  621 FECICGELGLYDYKARVQCLQCHLWQHAECVNYKQ--ENLGVTPFYCPHCLVAMK---PVPTGATLIISPSSICHQWVDEINRHVQ--SSSLHVMVYQGV---------KKHGFLQPQMLA-EQDVVITTYDVLRSELNYVD---IPHSNSEDGRKFRNQKRYMAVPSPLVAVEWWRICLDEAQMVECTTAKAAEMALRLSGINRWCVSGTPVQRG-LEDLFGLVLFLGVDPYWVKHWWDQLLYKPYCRKDPFP---LYKFISKIMWRSAKKDVLDQIQIPPQTEDVHWLHFSPVERHFYHRQHEVC---CQDALAKFRKITDWALKLSS-----LDRRTVS------SILYPLLRLRQACCHPQAVRGEFLPLQK--STMTMEELLTSLQKKCRTECEEAHRQLVCALNGLAGIHIIKGE 1001          

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|1069360850|ref|XP_018120713.1| (PREDICTED: E3 ubiquitin-protein ligase SHPRH-like [Xenopus laevis] >gi|1050375712|gb|OCT77910.1| hypothetical protein XELAEV_18029007mg [Xenopus laevis])

HSP 1 Score: 295.819 bits (756), Expect = 7.422e-77
Identity = 169/421 (40.14%), Postives = 238/421 (56.53%), Query Frame = 0

Query:  663 YVCPCGRD--RRGEAWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGE 1081
            + C CG       +A +QC  C  W H  C       E   +  F C  C    +   P P  ATLI++P  IC QW +E    +Q  S+ L ++ Y+GV         ++   L P +L  E D+V+TT+DVL  +L + D   +P S  E GR+ RN+K+Y   PSPL ++ WWRICLDEAQM+E + A AA MALRL  ++RWCVSGTP+ +G L+DL+GL+LFL V P+  + WW   +   + + DP     L++ +S IMWR++KK VLDQ+ +PPQTE    LHFS VER +Y  Q++ C    +D + K++++        S +   L +R +S      S   PLLRLRQACCHPQ   G    L+K    MTM+E+L  L++  +  CEE  R L+  L+GL  +  IKGE
Sbjct:  640 FECICGELGLYDYKARVQCLQCHLWQHAECVNYKQ--ENLGVTPFYCPHCLVAMK---PVPTGATLIISPSSICHQWVDEINRHVQ--SSSLHIMVYQGV---------KKHGFLQPQMLA-EQDVVITTYDVLRSELNYVD---IPHSNSEDGRKFRNQKRYMAVPSPLVAVEWWRICLDEAQMVECTTAKAAEMALRLTGINRWCVSGTPVQRG-LEDLFGLVLFLGVDPYWVKHWWDQLLYKPYCRKDPFP---LYKFISKIMWRSAKKDVLDQIQIPPQTEDVHWLHFSPVERHFYHRQHEVC---CQDALAKLRKITDW-----SLKLSSLDRRTVS------SILYPLLRLRQACCHPQAVRGEFLPLQK--STMTMEELLTSLQKKCRTECEEAHRQLVCALNGLAGIHIIKGE 1020          

HSP 2 Score: 126.716 bits (317), Expect = 3.856e-25
Identity = 75/242 (30.99%), Postives = 122/242 (50.41%), Query Frame = 0

Query: 1909 VLPCAHHLCKTCLRQLL------GKRAQNKCPKCRRNFHRKACAEVQESREGECKDMEEDRKDIRVGEDELYMDEAVQEEGDEEEEEEEEEDDEGPRVEGSWGTKITALVSEVLRLPVED---KCLIFSEWDEMLDIVQQALQTNRIDIVRLKGERTAGRVRQEFRENESVRCMLLNLKSGAKGLTLVEANHVFLLEPIFNAATEAQAVNRVFRIGQTKPTVIHRFVVRNTIEENVEKMRQS 2141
            VL C H  C  C+  ++       +R+  KC  CR+    K  + V  S                             E G++E++           V+GS  TK+ A+V  +L++ ++D   K L+FS W ++LDI+ +ALQ N +   ++ G          F+ +  +  +LL L +G+ GL ++EA HV L+EPI N A E QA+ RV RIGQTK T++HRF+++ TIE+ ++ M ++
Sbjct: 1426 VLTCGHCFCNECVAIIVDQYSVGTRRSAIKCAICRQTTSHKEISYVFTS-----------------------------EGGNQEQDIP---------VKGSHSTKVEAVVRTLLKIQIQDPGAKSLVFSTWQDVLDIIAKALQDNSLVFSQINGIHKFQENLSAFKYDPKINILLLPLHTGSNGLNIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKSTIVHRFLIKATIEDRMQAMLKT 1629          

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|260818236|ref|XP_002604289.1| (hypothetical protein BRAFLDRAFT_125252 [Branchiostoma floridae] >gi|229289615|gb|EEN60300.1| hypothetical protein BRAFLDRAFT_125252 [Branchiostoma floridae])

HSP 1 Score: 295.434 bits (755), Expect = 9.520e-77
Identity = 219/661 (33.13%), Postives = 326/661 (49.32%), Query Frame = 0

Query:  439 EPSVPGLKVTLRPYQARAVEWMQCREQARDYFAPRAPSPSGSHAENPEDDMDEENLLGHGWIKLLLPNPVPDPSASFVYYNPISVSLSHRWPVMWEPRALKGGILADEMGLGKTIEVMALVLSHRYLGGEKAAKEAKGSSLGCHEK-RGRGNPEKGGNCAPMHNGSPNKEAISL-DEDRIVKEGDGNDGGGGNPGREGSGGKDRKRGHDERRHRGRAEEGLVDRTE---------YVCPCGR-----DRRGEAWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVP--ATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGE 1081
            +P    L  +LRPYQ  AV WM   EQ  ++    +    G  AE           L   W ++ +P+         +++N  + SL         P  + GGILADEMGLGKT+E +AL+L H   G  +       S+ GC E+ + R   E+           P +E   + D+ +     D           + +    + +G   ++     E   +   +         + C CG      D R    LQC  C +W H  C   ++++E++  PA + E  H      S   VP  ATLI++P  I  QW +E    +++  A ++++ Y GV         +++  L P VL + +DIV+TT+DVL  +L + D   +P S    GR+LR +K+Y   PSPL S+ WWR+CLDEAQM+E + A AA MALRL  V+RWCV+GTPI + NL+DLYGLLLFL V P+  + WW   +   +  G+P     ++ ++S +MWRT+KK VLDQ+ LP QTE    L FS VE  +Y+ ++++C      V+         Q      +  +L +R       +++   PLL+LRQACCHPQ   G    L K    MTM E+L+ L +  K+ CEE  R L+  L+GL  +  IK E
Sbjct:  394 DPQHHLLVPSLRPYQKEAVLWMLEMEQFGEHLQQES---RGRAAE-----------LHFLWREVQMPD------GQILFFNSYTGSLCKEKQCGMVP--IPGGILADEMGLGKTVEALALMLLHPRPGLNRTT-----SATGCAEQDKNRTAVEQ-----------PKEERQEIQDQQQSTSSTDARQPKCQKKSSKKTASNSKSKGKKSKKGLAVVEPVCIPAPDSCNVQSTQRFECICGVTDAHVDSRTR--LQCWQCGNWQHAECVNYNATSESD--PAKMAEPYHCPHCSVSLPAVPSGATLIISPAPISHQWVDEINRHVRK--AAIKMLVYSGV---------KKQGFLQPKVLAD-HDIVITTYDVLRLELNYVD---IPHSNSMEGRRLRQQKRYMAVPSPLPSVEWWRVCLDEAQMVECTTAKAAEMALRLSAVNRWCVTGTPIQR-NLEDLYGLLLFLGVDPYWVKHWWERLLYQPYCHGNPLP---MYEVISKVMWRTAKKDVLDQINLPQQTEDVHWLKFSPVEEHFYRRKHEDCSRHAFKVL--------SQWHDLGTKLCQLDRR------TMQTMLWPLLKLRQACCHPQAVRGEFLPLHK--STMTMDELLKSLTQKCKIECEEAHRQLICALNGLAGIHIIKEE 977          

HSP 2 Score: 129.413 bits (324), Expect = 5.847e-26
Identity = 161/667 (24.14%), Postives = 264/667 (39.58%), Query Frame = 0

Query: 1528 SASQALDALDGEIDKLRKRGGKAAGQGRIVWWEWTLNELMVRGDLLDPKADLLRRLKVESSGPQFP-------AFHDYHGLMMAIKLKSKDLDETRTRVNALLRRVLVDNPSEGERQRNSDCGVCRKDWGKSGPLCHICELEKHLKAYEEKLHAYREQRQQNGHTV------------GTFKHSCCTHRLLYSVLLPWLRHQALKRKSNPEWQHAW-EEAMVDNRKEELLERERKMAKRFWNSGFDLLSKLDEV--ACSALSLELAQEGEIFKDMSSEQRKLVVFPHEVDVKKEEYRMQVAKYEVELRERKGYLKYLQNIAKNEARAAERNAAMARKMQKEVKSSASSERKDGAFREGPRIPTSIDPCASSGILQGNDEEGEEDNVNLCFVCFEEYTVINPREVLPCAHHLCKTCLRQLLG------KRAQNKCPKCRRNFHRKACAEVQESREGECKDMEEDRKDIRVGEDELYMDEAVQEEGDEEEEEEEEEDDEGPRVEGSWGTKITALVSEVLRLPVED---KCLIFSEWDEMLDIVQQALQTNRIDIVRLKGERTAGRVRQEFRENESVRCMLLNLKSGAKGLTLVEANHVFLLEPIFNAATEAQAVNRVFRIGQTKPTVIHRFVVRNTIEENVEKMRQSKVAAAGGESAVLSAVKKQGKTR 2163
            S+ Q L  +  ++ +LR++      Q    WW  TL   +  G       DL  R++ E SG + P        F D  GL   +  + ++L ++ + V   +   L  +P++   Q   +C + R + G+    C  C+ +     YE +L ++  +R ++                G +  S  T + L  VL       +  + S  E Q  W EE  +     E L RE K     W +  D ++ +DE+  A + L L L +E     + +   +  ++ P EVD +  +     A     L  + G L YL+N+AK                       +   +KDG                               N   C +C  +  +     VL C H  C+ C+  L+       +R   +C  CR+                                        +   GD      E +  E   V+GS  TK+ A+V  +  +  +D   K L+FS W ++LD+V  AL+ N ++   + G         +F+ +  +  +LL L +G+ GL ++EA HV L+EPI N A EAQA+ RV RIGQTK TV+HRF+V+NTIEE +  M++   A+   ++     V   G  R
Sbjct: 1054 SSQQTLLPIQQKVQQLRRKL-----QPGTPWWMDTLQWAIYWGK----DEDLAERIRNELSGDRDPDATSIVNKFRDLRGLQFLLTSQLENLQQSHSAVCTAVNH-LSQDPAQRLIQSAVECHL-RPEKGEELHTCEFCKADDLFLEYESRLFSFTAKRLEDMVAEEEENPERQVTRRGVWAQSE-TEKCLKIVL-------SFCKSSRLEEQ--WVEEGNMQVELFEQLRREYKQLHVVWMALRDRVAAIDELDMATTRLRLRLPEE-----ERTDPPQPNIIEPGEVDHQWIKNLNDKAVATSLLHRKLGQLLYLKNLAK-----------------------SQEYQKDGV------------------------------NPEPCPIC--QRQLGTEWSVLMCGHCFCEECISVLVNQFGLGSRRGSIRCAICRQ----------------------------------------LTATGDISYVSTEAKQQEDVDVKGSHSTKVEAVVRTLKLIRQKDPTAKSLVFSTWQDVLDVVATALRENNMEYRAINGIHRFQENITDFKYDSGIGVLLLPLHTGSHGLNIIEATHVLLVEPIINPAQEAQAIGRVHRIGQTKATVVHRFLVQNTIEERIHAMKKHISASDSIDTQSEGTVLTVGDLR 1599          

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|533150032|ref|XP_005389286.1| (PREDICTED: E3 ubiquitin-protein ligase SHPRH [Chinchilla lanigera])

HSP 1 Score: 294.664 bits (753), Expect = 1.454e-76
Identity = 183/512 (35.74%), Postives = 266/512 (51.95%), Query Frame = 0

Query:  584 KRGRGNPEKGGNCAPMHNGSPNKEA-----ISLDEDRIV----KEGDGNDGGGG-NPGREGSGGKDRKRGHDERRHRGRAEEGLVDRTEYVCPCGR----DRRGEAWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGE 1081
            K+G+  P+ G +   +H+  P         +++ E   V    +E +  D G   NP    S   +    +D   +R            + C CG     DR+    +QC  C  W H +C       E   +  F C  C        P    ATLI++P  IC QW +E    I+  S+ LRV+ Y+GV +            L P  L  E DIV+ T+DVL  +L + D   +P S  E GR+LRN+K+Y   PSPL ++ WWRICLDEAQM+E  A  AA MALRL  ++RWC+SGTP+ +G L+DL+GL++FL + P+  + WW   ++LL+          L+  ++ I+WR++KK V+DQ+ +PPQTE T  LHFS VER +Y  Q++ C    +D V K++++     + +S     L +R ++      S   PLLRLRQACCHPQ   G    L+K    MTM+E+L  L++     CEE  R L+  L+GL  +  IKGE
Sbjct:  584 KKGKSQPDVGAD---IHDHCPATSDSGITDVTMSEGTCVSEVREEQEAKDHGESLNPATNESSHSNVTSPYDNADYR------------FECICGEFDQIDRKPR--VQCLNCHLWQHAKCVNYEE--ENLKIKPFYCPHCLVA---MEPVSTRATLIISPSSICHQWVDEINRHIK--SSSLRVLVYQGVKKDG---------FLQPHFLA-EQDIVIITYDVLRSELNYVD---IPHSNSEDGRRLRNQKRYMAIPSPLVAVEWWRICLDEAQMVECPAVKAAEMALRLSGINRWCISGTPVQRG-LEDLFGLVVFLGIEPYSVKHWW---VQLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQIPPQTEETHWLHFSPVERHFYHRQHEVC---CQDAVVKLRKISDWALKLSS-----LDRRTVT------SILYPLLRLRQACCHPQAVRGEFLPLQK--STMTMEELLTSLQKKCGTECEEAHRQLVCALNGLAGIHIIKGE 1038          

HSP 2 Score: 133.65 bits (335), Expect = 3.395e-27
Identity = 87/283 (30.74%), Postives = 134/283 (47.35%), Query Frame = 0

Query: 1909 VLPCAHHLCKTCLRQLL------GKRAQNKCPKCRRNFHRKACAEVQESREGECKDMEEDRKDIRVGEDELYMDEAVQEEGDEEEEEEEEEDDEGPRVEGSWGTKITALVSEVLRLPVED---KCLIFSEWDEMLDIVQQALQTNRIDIVRLKGERTAGRVRQEFRENESVRCMLLNLKSGAKGLTLVEANHVFLLEPIFNAATEAQAVNRVFRIGQTKPTVIHRFVVRNTIEENVEKMRQSKVAAAGGESAVLSAVKKQGKTRKTDVELDVEEMEKLFATTP 2182
            VL C H  C  C+  ++        R   KC  CR+    K  + V  S                                   E+  +EED     V+GS  TK+ A+V  ++++   D   K L+FS W ++LDI+ +AL  N ++  ++   +T       F+ +  +  +LL L +G+ GLT++EA HV L+EPI N A E QA+ RV RIGQTKPT++HRF+++ TIEE ++ M +         +A  S +   GK  +  V L V ++  LF   P
Sbjct: 1444 VLTCGHCFCNECIAIIIEQYSVGSHRTSIKCAICRQTTSHKEISYVFTS-----------------------------------EQANQEEDIP---VKGSHSTKVEAVVRTLMKIQRTDPGAKALVFSAWQDVLDIISKALTDNNMEFAQISRVKTFQENLSAFKHDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLK---------TAERSHMNSSGKHSEASV-LTVADLADLFTKEP 1678          

HSP 3 Score: 66.2402 bits (160), Expect = 8.662e-7
Identity = 45/120 (37.50%), Postives = 62/120 (51.67%), Query Frame = 0

Query:  443 PGLKVTLRPYQARAVEWMQCREQARDYFAPRAPSPSGSHAENPEDDMDEENLLGHGWIKLLLPNPVPDPSASFVYYNPISVSLSHRWPVMWEPRALKGGILADEMGLGKTIEVMALVLSH 562
            P L   LRPYQ  AV WM  +E+ R      AP+               E+ L   W +++ P  +       +YYNP +  +   +P    P+ L GGILADEMGLGKT+EV+AL+L+H
Sbjct:  297 PALTPVLRPYQREAVNWMLQQERFRS-----APT--------------NESSLHFLWREIITPEGLK------LYYNPYTGCIIREYP-HSGPQLL-GGILADEMGLGKTVEVLALILTH 389          

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|677224647|gb|KFP51149.1| (E3 ubiquitin-protein ligase SHPRH [Cathartes aura])

HSP 1 Score: 288.886 bits (738), Expect = 6.112e-76
Identity = 166/421 (39.43%), Postives = 239/421 (56.77%), Query Frame = 0

Query:  663 YVCPCGRDRRGE--AWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGE 1081
            + C CG     +  A +QC  C  W H  C       E   +  F C  C    +   P    ATLI++P  IC QW +E    ++  S+ LRV+ Y+GV         ++   L P +L  E ++V+TT+DVL  +L + D   +P S  E GR+ RN+K+Y   PSPL ++ WWRICLDEAQM+E + A AA MALRL  ++RWCVSGTP+ +G L+DLYGL+LFL V P+  + WW   +   + + +P     L+ L++ IMWR++KK V+DQ+ +PPQTE    LHFS VER +Y  Q++ C    +D + K++++     + +S     L +R ++      S   PLLRLRQACCHPQ   G    L+K    MTM+E+L  L++  +  CEE  R L+  L+GL  +  IKGE
Sbjct:  656 FECICGELGLADYKARVQCLKCYLWQHAECVNYKE--ENLKIKPFYCPHCLVAMK---PVSTGATLIISPSSICHQWVDEINRHVR--SSSLRVLVYQGV---------KKHGFLQPHMLA-EQEVVITTYDVLRTELNYVD---IPHSNSEDGRRFRNQKRYMAIPSPLVAVEWWRICLDEAQMVECTTAKAAEMALRLSGINRWCVSGTPVQRG-LEDLYGLVLFLGVDPYWVKHWWDQLLYRPYCRKNPQP---LYSLIAKIMWRSAKKDVIDQIQIPPQTENVHWLHFSPVERHFYHRQHEVC---CQDALAKLRKISDWTLKLSS-----LDRRTVT------SILYPLLRLRQACCHPQAVRGEFLPLQK--STMTMEELLTSLQKKCRTECEEAHRQLVCALNGLAGIHIIKGE 1036          

BLAST of EWM26996.1 vs. NCBI_GenBank
Match: gi|699700424|ref|XP_009907117.1| (PREDICTED: LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SHPRH [Picoides pubescens])

HSP 1 Score: 292.738 bits (748), Expect = 6.500e-76
Identity = 167/421 (39.67%), Postives = 240/421 (57.01%), Query Frame = 0

Query:  663 YVCPCGRDRRGE--AWLQCDACKHWAHVRCAGLSSSAEAEALPAFVCEVCHGLERFQSPSPVPATLIVTPQLICGQWEEEFKSRIQEGSAQLRVVTYRGVAETSRSWEWEERRLLDPDVLGNEYDIVLTTFDVLAKDLGHTDSCFLPPSRGETGRQLRNRKKYRVQPSPLASMVWWRICLDEAQMIEASAAAAARMALRLKTVHRWCVSGTPIWKGNLDDLYGLLLFLQVSPFDRRSWWRHAIELLHEKGDPDAASRLHRLLSAIMWRTSKKSVLDQLALPPQTERTRILHFSQVERAYYQTQYKECIGKVRDVVRKIKRMQQQQQQRTSGRAQELTKRKLSATTALESCAGPLLRLRQACCHPQIGAGGLGGLRKGRGIMTMQEVLEKLEEDEKLRCEETQRLLMMELSGLGSMARIKGE 1081
            + C CG     +  A +QC  C  W H  C       E   +  F C  C    +   P P  ATLI++P  IC QW +E    ++  S+ LRV+ Y+GV         ++   L P +L  E D+V+TT+DVL  +L + D   +P S  + GR+ RN+K+Y   PSPL ++ WWRICLDEAQM+E + A AA MALRL  ++RWCVSGTP+ +G L+DLYGL+LFL V P+  + WW   +   + + +P     L+ L++ IMWR++KK V+DQ+ +PPQTE    LHFS VER +Y  Q++ C    +D + K++++     + +S     L +R ++      S   PLLRLRQACCHPQ   G    L+K    MTM+E+L  L++  +  CEE  R L+  L+GL  +  IKGE
Sbjct:  671 FECICGELGLADYKARVQCLKCYLWQHAECVNYKE--ENLKIKPFYCPHCLVAMK---PVPTGATLIISPSSICHQWVDEINRHVR--SSSLRVLVYQGV---------KKHGFLQPHMLA-EQDVVITTYDVLRTELNYVD---IPHSNSKDGRRFRNQKRYMAIPSPLVAVEWWRICLDEAQMVECTTAKAAEMALRLSGINRWCVSGTPVQRG-LEDLYGLVLFLGVDPYWVKHWWDQLLYRPYCRKNPQP---LYSLIAKIMWRSAKKDVIDQIQIPPQTENVHWLHFSPVERHFYHRQHEVC---CQDALAKLRKISDWTLKLSS-----LDRRTVT------SILYPLLRLRQACCHPQAVRGEFLPLQK--STMTMEELLTSLQKKCRTECEEAHRQLVCALNGLAGIHIIKGE 1051          

HSP 2 Score: 124.02 bits (310), Expect = 2.682e-24
Identity = 73/242 (30.17%), Postives = 119/242 (49.17%), Query Frame = 0

Query: 1909 VLPCAHHLCKTCLRQLL------GKRAQNKCPKCRRNFHRKACAEVQESREGECKDMEEDRKDIRVGEDELYMDEAVQEEGDEEEEEEEEEDDEGPRVEGSWGTKITALVSEVLRLPVED---KCLIFSEWDEMLDIVQQALQTNRIDIVRLKGERTAGRVRQEFRENESVRCMLLNLKSGAKGLTLVEANHVFLLEPIFNAATEAQAVNRVFRIGQTKPTVIHRFVVRNTIEENVEKMRQS 2141
            VL C H  C  C+  ++       +R+  KC  CR+    K  + V  +                                     E   ++D+ P V+GS  TK+ A+V  + ++  +D   K L+FS W ++LDI+ +AL  N +   ++ G          F+ + ++  +LL L +G+ GL ++EA HV L+EPI N A E QA+ RV RIGQTK T++HRF+++ TIEE ++ M ++
Sbjct: 1457 VLTCGHCFCNECIAIIIEQYSVGTRRSSIKCAICRQTTSHKEISYVFTA-------------------------------------ETANQEDDIP-VKGSHSTKVEAVVRTLKKIQFKDPGAKSLVFSTWQDVLDIISKALYDNNMIFSQINGISKFQENLSAFKYDPNINILLLPLHTGSNGLNIIEATHVLLVEPILNPAHELQAIGRVHRIGQTKSTIVHRFLIKATIEERMQTMLKT 1660