EWM27219.1, cds3532 (CDS) Nannochloropsis gaditana

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|585109399|gb|EWM27219.1| (abc transporter g family member 29 [Nannochloropsis gaditana])

HSP 1 Score: 3413.24 bits (8849), Expect = 0.000e+0
Identity = 1658/1658 (100.00%), Postives = 1658/1658 (100.00%), Query Frame = 0

Query:    1 MASIDGSGKPDAPGDQLVEKEREVQELRRILEQERALARHWRYVASHAAHQNELPIKASSPSIDGHPGTVLPTLTVSEPPPRKSSFVALPLTAPTQLEGATGVNDEKNAAGGILTTSELDLKRLMGTSVIREIGVENHFKNLASTLGESRRNISSTSVQIQDLSLSYMVEQSNKRFLNVGEALLNPFLALLRSGGKKQTFQLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGALVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLNLRTEAVATRFGALYQSLLTIAFQTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEFIAKNYGALLPGSTETIGETVLGQKGLFTNARWVWLGLLANLGITLFYLCLTLVAMMYLEWLQPVSSADTTPEEAIAQSSLALRGPGLLRRQTTERHLELDREESDVDEDGYSWTSESVQSWGVESSTNTITAFRSFSGLGGGAEDDEDASSTNTSERGGARQGVMGSSVDEETKEAEGLAKDDARGVKFSGQVAEHTYGKETVRPKNENLAHSVLPRAVSSVKLLADLRNGKSSTWQLEAVKAIPFVPTALTFENLWYSVPVNGNETVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLPKEVSASKCRHFVGDVLTLLELDPIANAVVGPPGAGLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFRQTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGNDNQGSIGFFSIKNKQFEELTRWEFVKDFFGTFALEHRFRNIGVLIAFTEDWIMHWQDSQDSSHEELHYSDGGLRDVYKTIIGDL 1658
            MASIDGSGKPDAPGDQLVEKEREVQELRRILEQERALARHWRYVASHAAHQNELPIKASSPSIDGHPGTVLPTLTVSEPPPRKSSFVALPLTAPTQLEGATGVNDEKNAAGGILTTSELDLKRLMGTSVIREIGVENHFKNLASTLGESRRNISSTSVQIQDLSLSYMVEQSNKRFLNVGEALLNPFLALLRSGGKKQTFQLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGALVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLNLRTEAVATRFGALYQSLLTIAFQTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEFIAKNYGALLPGSTETIGETVLGQKGLFTNARWVWLGLLANLGITLFYLCLTLVAMMYLEWLQPVSSADTTPEEAIAQSSLALRGPGLLRRQTTERHLELDREESDVDEDGYSWTSESVQSWGVESSTNTITAFRSFSGLGGGAEDDEDASSTNTSERGGARQGVMGSSVDEETKEAEGLAKDDARGVKFSGQVAEHTYGKETVRPKNENLAHSVLPRAVSSVKLLADLRNGKSSTWQLEAVKAIPFVPTALTFENLWYSVPVNGNETVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLPKEVSASKCRHFVGDVLTLLELDPIANAVVGPPGAGLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFRQTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGNDNQGSIGFFSIKNKQFEELTRWEFVKDFFGTFALEHRFRNIGVLIAFTEDWIMHWQDSQDSSHEELHYSDGGLRDVYKTIIGDL
Sbjct:    1 MASIDGSGKPDAPGDQLVEKEREVQELRRILEQERALARHWRYVASHAAHQNELPIKASSPSIDGHPGTVLPTLTVSEPPPRKSSFVALPLTAPTQLEGATGVNDEKNAAGGILTTSELDLKRLMGTSVIREIGVENHFKNLASTLGESRRNISSTSVQIQDLSLSYMVEQSNKRFLNVGEALLNPFLALLRSGGKKQTFQLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGALVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLNLRTEAVATRFGALYQSLLTIAFQTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEFIAKNYGALLPGSTETIGETVLGQKGLFTNARWVWLGLLANLGITLFYLCLTLVAMMYLEWLQPVSSADTTPEEAIAQSSLALRGPGLLRRQTTERHLELDREESDVDEDGYSWTSESVQSWGVESSTNTITAFRSFSGLGGGAEDDEDASSTNTSERGGARQGVMGSSVDEETKEAEGLAKDDARGVKFSGQVAEHTYGKETVRPKNENLAHSVLPRAVSSVKLLADLRNGKSSTWQLEAVKAIPFVPTALTFENLWYSVPVNGNETVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLPKEVSASKCRHFVGDVLTLLELDPIANAVVGPPGAGLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFRQTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGNDNQGSIGFFSIKNKQFEELTRWEFVKDFFGTFALEHRFRNIGVLIAFTEDWIMHWQDSQDSSHEELHYSDGGLRDVYKTIIGDL 1658          

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|971519959|dbj|GAQ78290.1| (pleiotropic drug resistance protein [Klebsormidium nitens])

HSP 1 Score: 629.017 bits (1621), Expect = 0.000e+0
Identity = 454/1456 (31.18%), Postives = 714/1456 (49.04%), Query Frame = 0

Query:  149 SRRNISSTSVQI--QDLSLSYMVEQSNKRFLNVGEALLNPFLALLRS----GGKKQTFQLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGALVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLNLRTEAVATRFGALYQ-----SLLTIAFQTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEFIAKNYGALLPGSTETIGETVLGQKGLFTNARWVWLGLLANLGITLFYLCLTLVAMMYLEWLQPVSSADTTPEEAIAQSSLALRGPGLLRRQTTERHLELDREESDVDEDGYSWTSESVQSWGVESSTNTITAFRSFSGLGGG--AEDDEDASSTNTSERGGARQGVMGSSVDEETKEAEGLAKDDARGVKFS--GQVAEHTYGKETVRPKNENLAH-SVLPRAVSSVKLLADLRNGKSSTWQLEAVKAIPFVPTALTFENLWYSVPV------NGNE--TVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLPKEVSASKCRHFVGDVLTLLELDPIANAVVGPPG-AGLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFR--QTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFS---FMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGN 1574
             R  ++   V+I  ++L++   V   ++   N+  A  N   ++ R+       ++ FQ+  L+ ++    P ++ L++G P CGK++LL A+A R    +  +++GS+ YNG  A    F+      +V +VD +   LTVRETL F   C      + L++ + GR  V++  V +           +G  K          + VM +LGL  CADT++G+   RGVSGG+R+RVT  E+L+G   V  LDEIS GLDS+  + IV   R          + +L QPAPE   LFD V++L +G ++YQGP +    +FE  GF+CPPR  +ADFL  VT    +    S D        P  V        F   F   E G+ + +E ++ P K   S I +++                 + K+       F  C  RE+ L  R +   + +  Q   +  +T  VF          + G+LY      SL+ I F + A +     R  +F+KQ+   F+   ++ L +     P  L++ +++  L Y+  G A D     FF+++  L  +    +  F+L   L   +T A  A       + L  GFII+   +  ++ W  +++PL Y  QG+A+NEF A  +  L PG+ ET+G  +L  +GLF    WVW+ +   +G  + +  L   A   L++  P+      P+  I +  L                   ++E++ V  D     ++S++S  V          R+ +G  G   A  DE+ ++ + +    A  G      + E       ++  + G+  S  GQ +  + GK  V+ ++  LA   V P  +S     + L      +   +    +PF P ++ F N+WYSV V       G E   + +LKG SG+  PG + AL+G SGAGKTTL+DVLAGRKT G I G+I V G P   D F  +S YVEQF   SP  TV EAL F+A LRL K+V     + FV +V+ L+EL P+ NA+VG PG +GL  E  KR+TI  E+VANPSI+F+DEPT+GLDARAA +V+R +R    TGR ++CTIHQPS +IF  FD LLL+KRGG  ++ GPLG  S  LI++F ++ G PA+  G NP+TWMLE    G      +    Y   ++ S+L   N E +  L + P++   A+      +    FG Q   C  +  R  W+ PEY   R   S  + LI GS+++   Q+++T   + + M  L+ +   +   N+++V  V A E+    RE    MY   A+ L+    EIP+VL+QT++YS   Y   N+    W    FF ++   F+  L  T +G    A  P + ++ I+ +     +++ +G +I    +PG W + + + P++    GL+ +Q G+
Sbjct:  100 DRVGVTQPKVEIRYENLTIEAGVYVGSRALPNLLNAARNSMESIFRTLRLTKDTRRRFQI--LRDVSGVLKPGRLTLLLGPPGCGKSTLLLALAQR--LDSNLKVTGSISYNGHSARE--FYPKRTVTYVPQVDLHMGELTVRETLDFGARCQGVGANQDLIR-ELGRREVQNGVVPDPDIDAFMKAISLGNQKENLV-----TDYVMKILGLDICADTLVGNQLIRGVSGGQRKRVTTGELLVGLKKVLLLDEISTGLDSSTTYQIVNCLRNICHFTDSTVMTSLLQPAPETFELFDDVVLLSEGHVIYQGPREHILAFFETQGFKCPPRKGIADFLQEVTSRKDQEQYWSRD--VPYKYVPVSV--------FAAAFNDFEVGQAIRKELSVPPEK---SEIPSDAL---------------AKTKYGAPQYEIFKACLDREWTLMRRTKFLYIFKTFQVAVMAFMTATVFFRTTLNPDNIQDGSLYLSMIFFSLVHIMFNSYAEMSLTVQRLPIFFKQREDQFYPGWAFNLPAIITRLPYTLVEVLIWTALTYYIVGLAPDAG--RFFIYILILLALHQMGVGLFRLVGVLARTMTIANTAGSFALLCIFLLGGFIIAKPQIHPWWIWGFWISPLSYVQQGMAINEFSAPRWQKLAPGTNETLGSIILTSRGLFHQHWWVWVSVGVMVGYAVIFNLLINAA---LDYFPPIGK----PQAIIPEDVLD------------------EKEKARVGHD-----NDSLKSVKVPVDALQEQQARNGTGTDGNAIASVDEETAAQHETAVSLAPPG-----AEIELAPGRSASRRGSFGLTRSGLGQPSSSSLGKGKVKQQSSGLAQFKVQPSGLSQQS--SGLAQYYEQSGAAKRGMVLPFQPLSMVFHNVWYSVDVPKGLAKEGEEGKKLQILKGCSGAFRPGVLTALVGVSGAGKTTLMDVLAGRKTTGYIEGDIFVGGFPKEQDSFARVSGYVEQFDIHSPSTTVFEALTFSAWLRLAKDVDPQTRKEFVEEVMDLVELTPLRNALVGSPGVSGLPVEARKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVDTGRTVVCTIHQPSIDIFEAFDELLLMKRGGYVIYAGPLGTHSKFLIEHFSAVKGVPAIRDGYNPSTWMLEVTTPGMEEKLDLDFAEY---YKNSSLFKRNEELIGKLKR-PAEGASAI--RFDAKHARDFGVQFAACFWKNSRIYWQTPEYNGVRYFFSTGVALIFGSIFWDLGQSRNTQQNVFNVMGALYASVLFLGVSNASSVQPVFATERAVSYRERGAGMYSPTAWVLALCAIEIPYVLVQTIVYSVITYAMINFE---WTAVKFFEYTTVMFLTFLIFTYYGIMAVAGTPGLALSSIISSFFYSFWNLFAGFLIPLPQIPGWWVWYYYINPISWTLYGLIASQLGD 1467          

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|971507978|dbj|GAQ90231.1| (ATP-binding cassette transporter [Klebsormidium nitens])

HSP 1 Score: 615.15 bits (1585), Expect = 0.000e+0
Identity = 452/1503 (30.07%), Postives = 710/1503 (47.24%), Query Frame = 0

Query:  136 ENHFKNLASTLGESRRNISSTSVQIQDLSLSYMVEQSNKRFLNVGEALLN----PF--LALLRSGGKKQTFQLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRAC------RGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGALVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVF----LNLRTEAVATRFGALYQSLLTIAFQTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEFIAKNYGALLP-GSTETIGETVLGQKGLFTNARWVWLGLLANLGITLFYLCLTLVAMMYLEWLQPVSSADTTPEEAIAQSSLALRGPGLLRRQTTERHLELDREESDVDEDGYSWTSESVQSWGVESSTNTITAFRSFSGLGGGAEDDEDASSTNTSERGGARQGVMGSSVDEETKEAEGLAKDDARGVKFSGQVAEHTYGKETVRPKNENLAHSVLPRAVSSVKLLADLRNGKSSTWQLEAVKAIPFVPTALTFENLWYSVPV-------NGNETVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLPKEVSASKCRHFVGDVLTLLELDPIANAVVGPPGA-GLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFR---------QTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGNDNQGSIGFFSIKNKQFEELTRWEFVKDFFG 1604
            E   + L   +      + +  V+ +DL++S  V   ++   ++  A LN    P   L LL+S   K+ F +  L+ +     P +M L++G P  GKT+LL  +AG++   +   ++G + YNG +     F    ++ ++ + D +   LTVRET  F+     P    +++     R   + +  D      ++A       R VG             E ++ +LGL   ADT+IG+   RG+SGG+++RVT  E+++G      +DEIS GLDS+  F IV+  R      +   ++AL QPAPE   LFD VL+L +G +V+ GP  E   +FE LGF  P R  +ADFL  VT                            S KD  Q + + EP R +   A  +  K++    +     S   +  +      E+    N W   F     RE  L  R+R     R +Q   +  +T  +F    L +  +      G L+ SL+ + F   + +     R  VFY+Q++  F++  +  L +     P    +A ++  +VY+  G A       FF +   + +V    ++ F+L   L   +  A          + L  GFI+S  D+  ++ W  +++PL Y    +AVNEF A  +    P   +ET+G  +L Q+GL T   W+W+G    +G    +  L  +A+ YL  L  V +    PE+A+ +      G G  +         L    S  DEDG      + +      S   I+          GAE D +        R G R    G++  E+     G+   + R    +G+  E T  K  +   + +L   +   +++S K  + L+   S     +    +PF P A+TF ++ Y+V +         ++ + LL  +SG+  PG + ALMG SGAGKTTL+DVLAGRKT G I GEI V G P   + F  +S YVEQ    SP +TV E+L+F+A LRL +++       FV +V+ L+EL  I NA+VG PG+ GLS E+ KR+TI  E+VANPSI+F+DEPT+GLDARAA +V+R +R    TGR ++CTIHQPS +IF  FD LLLLKRGG+T++ GPLG DS  L+ YF+ +PG P + P  NPA WML+    G      V   +   + +        IE        P+   + +    K  +      Q    L +   + WR PEY   R L   V+G+I GS+Y+          Q  +   GL  +  +LFL        N++ V  VVA+E+    RE    MY    Y ++  L E P+V+ QT++YSA  Y   ++     +  W+ +F F+ L+  T +G    A+ P+VQ+A ++ +    IF++ +G ++    +PG W + W L P+     GLV +Q G+ +     F + +N Q   +   +F+  +FG
Sbjct:   96 EKFLRKLRERIDRVGIQLPTVEVRYEDLNISAKVYVGSRALPSLTNAALNLIQMPLQRLGLLKS--NKRDFPI--LRDVNGRLKPGRMTLLLGPPGSGKTTLLLTLAGKA--ASDLIVTGKITYNGVDIHE--FEPRRVSAYISQSDVHTGELTVRETFDFATRVQGPGYKTEMIDELTKREQSQKIAPDPDIDAFVKASTLPDKQRNVG------------TEYILRVLGLDYAADTVIGNEMMRGISGGQKKRVTTGEMIVGPKKALFMDEISTGLDSSTTFQIVKAVRNWTHLMEGTVLMALLQPAPEAFDLFDDVLLLSEGYVVFHGPVTEVLSFFESLGFTPPERKGIADFLQEVT----------------------------SKKDQAQYWNRREPYRFVPVSAFAAAFKESRWGQEIREDLSQPVQPAKPLALPKEKYAL-NRW-AMFRALLRRELILMKRNRFLYTFRTVQCGIVAFITATLFFRTTLGVNLQDGPIYLGVLFFSLIHMLFNGFSELAITVGRLPVFYRQRDNLFYTGWALNLPTAVLRIPYSFAEATIWTVIVYYVVGLA--PQADRFFTYWFLMVLVHSMALALFRLIAALGRKMVIANTGGSFALLVIFLLGGFILSKPDIKPWWVWGYWISPLAYAQNAIAVNEFTAPRWQRPNPQNPSETLGFAILRQRGLHTAKWWIWVGFATLIGFYFIFNILVSLALQYLPSLDSVQA--IVPEDALKEKHEIQHGTGSAK--------NLAPSGSRTDEDGDVEAGNADRGKAKAPSVPPISE-------ADGAEGDVEMG------RIGHRSSAAGAARMEDVN-GNGIVPVNGRP---NGE-RETTASKSGLGAPSRSLRQRL---SMTSSKRGSGLKQRSSLERVADKGMILPFQPLAMTFHHVNYAVDMPKEFQKEGQDKKLGLLNDISGTFRPGVLTALMGVSGAGKTTLMDVLAGRKTGGYIEGEIRVGGHPKVQETFARVSGYVEQNDIHSPQLTVLESLRFSAWLRLAEDIDKETRNEFVDEVMELVELTGIRNALVGLPGSSGLSVEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVNTGRTVVCTIHQPSIDIFESFDELLLLKRGGRTIYAGPLGKDSSELVKYFKHVPGVPDIPPRYNPAAWMLDSTTPGMEEKLHVDFADIFKQSDLYKHLERTIEE----ESKPAPGVEPLRFDKKYPQNPFV--QTAALLWKNNITYWRTPEYNFVRFLFCTVVGVIFGSIYWNLGTKRGNLGQVLNVAGGL--YAVVLFLGVN-----NASTVQPVVAVERGVSYRERAAGMYSAIPYAIAQGLIEFPYVMAQTIVYSAITYAMIHFQWTAAKFFWYTLFMFLTLMIFTFYGMMAVAITPNVQLAAVISSAFYSIFNLFAGFLLPRPRIPGWWVWAWYLDPVAWSLYGLVGSQLGDID---TEFITDQNGQIVSVA--DFIHRYFG 1497          

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|760448937|ref|XP_011401537.1| (Pleiotropic drug resistance protein 15 [Auxenochlorella protothecoides] >gi|675356077|gb|KFM28517.1| Pleiotropic drug resistance protein 15 [Auxenochlorella protothecoides])

HSP 1 Score: 574.318 bits (1479), Expect = 1.549e-173
Identity = 424/1404 (30.20%), Postives = 668/1404 (47.58%), Query Frame = 0

Query:  214 PQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKA-ETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGALVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLNLRTEAVA---TRFGALYQSLLTIAFQTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFFVF--LAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLF---SGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEFIAKNYGALLPGSTE---TIGETVLGQKGLFTNARWVWLGLLANLGITLFYLCLTLVAMMYL--EWLQPVSSADTTPEEAIAQSSLALRGPGLLRRQTTERHLELDREESDVDEDGYSWTSESVQSWGVESSTNTITAFRSFSGLGGGAEDDEDASSTNTSERGGARQGVMGSSVDEETKEAEGLAKDDARGVKFSGQVAEHTYGKETVRPKNENLAHSVLPRAVSSVKLLADLRNGKSSTWQLEAVK-AIPFVPTALTFENLWYSVPVNG---------------NETVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLPKEVSASKCRHFVGDVLTLLELDPIANAVVGPPG-AGLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFRQ--TQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFSF-MYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGN-DNQGSIGF 1582
            P ++ L++G P  GKT+ +KA+ GR    +  +++GSV YNG+      F    +A +V + D +   LTVRET+ F+  C +    + LL+T   R     +  D      +RA        I +  +   A E ++ LLGL  C+DT++G+   RG+SGG+R+R+   EV + +  V   DEIS GLDS   F+I    RA     +   ++AL QP+PE   LFD V+++  G + Y GP +     FE  G  CP R  VADFL  VT P                 S  Q  W  +T+ +           ++        + K   ++  E+  +   R  E    +    K+  ++ T F+    R   L  RD+ +V  R+ Q   +  +   +FL+     V+      GA + SL+ +     A      +R  VFYKQ+   F+    + + +     P   +DA L+  + Y+  GF        FFVF  L  LT   WA+ S  +  T           AS + +FFLL+F    GF++    +P ++    + NP  Y    +++NEF+  ++    P       ++GE VL  +   T+ R VW+G+ A L  T+  + + ++A  ++     +PV S +   E   A+     R P      +  R ++L    +   +      + S +  G    T  +T   S   L    ED     +      GG  +   G+     T  A+        G       A +        P + +LA                ++ G+ +   LE+V+ ++PF P A+TF+++ YSVP+                  + + LL G++G   PG + ALMG+SGAGKTTL+DVLAGRKT G++ GE+ VNG P     F  IS YVEQ     P  TV EA++F+A LRLP+ VSA+    FV +++ L+ELD +  A+VG  G +GLS +  KR+TI  E+VANPSI+F+DEPTTGLDARAA +V+  +R +  TGR ++CTIHQPS +IF  FD LLLLK GG+ VF GP+G +S  LIDYF + PG   +    NPA WMLE           V      A  + +      IE    +     D Q A M      E +    QL   LRR +   WR P+Y I R   + ++  + GSL + Q   +DT +G+++   LLF +T  +   N  +V  +   +++   RE    MY V  Y+++  + E+P++++QTL YS   Y    + +A     ++F+F F + L + TA+GQ    + P + +A +  +     +++M G +I    +PG W + + + P++     L+V+Q G+ D++  + F
Sbjct:  141 PGRLTLILGPPSSGKTTFMKALVGRYRHSSDLKVTGSVTYNGKNFNE--FMPERVASYVNQFDTHFGELTVRETIDFAAQCQSSSHMKDLLETVLAREKEAGVTPDPGVDAYMRA--------IAFGHSNSIAVEAIIRLLGLDTCSDTLVGNQMLRGISGGQRKRLGTGEVGVSQSLVLVADEISTGLDSNTTFNITNSLRAIAHIRRATLLVALLQPSPETYELFDDVILISGGLVCYHGPRESVADIFERFGLACPARKGVADFLQEVTTP-----------------SDQQKYWVDNTRAY----------EYVTASTIRDAYLKHPIALDMENELAAPYRP-EPGALDLPTYKYAVSYKTLFLSNLKRTLLLQTRDKLFVYVRVFQICLMSVVVATLFLSTPKTTVSDGNIYSGAAFFSLVYMLIGGLAESHLLTMRLPVFYKQREMMFYPGWCFAVPAFIFRLPFCFVDATLWSCIAYFAVGFDVSS---RFFVFWLLMFLTCAWWAIASVCRTDTI----------ASAVGSFFLLVFIVTGGFVMVKSAIPPWWIGAYWANPWAYLTMSISINEFMGASWDVPNPADPTNPLSLGEQVLAFRDFPTSTRMVWIGVAAGLASTIINVAVFVLATTFMPPRHSKPVMSEEALEELEFAREVAPQRPP----PSSVTRDIQLGIRRAASTQS----MAASAEEGGAGPRTPGMTPVPSALDLPAVPEDVSFTMAAGDRPVGG--RAAPGNGRRSPTDGADARHSSPGNGTDGHHSPAVNGANGRPASPTDSSLAF---------------MKGGEGAAKALESVRSSLPFRPLAMTFKDVSYSVPLPKGMDPGTADVPAQGPHKDALRLLSGINGIFRPGVLTALMGASGAGKTTLMDVLAGRKTGGTVTGEVHVNGYPKVQKTFNRISGYVEQEDIHLPQTTVGEAVRFSAALRLPRGVSAATRAAFVTEMMDLVELDRMDGALVGILGESGLSIQARKRLTIAVELVANPSIIFMDEPTTGLDARAASVVMATVRAVVNTGRTVVCTIHQPSIDIFEAFDELLLLKPGGRCVFAGPVGLESSQLIDYFMATPGVKPIKERYNPANWMLEQTNPHNESLLGVDFSEVFAGSKQARENEKLIEAAHAVAPGMPDLQYADM------EVSGAWVQLGHLLRRNFVMYWRAPDYNITRFGVTLLVAFVFGSLAWEQGEARDTTSGVLNIAGLLFASTLFIGVSNCMSVQPIAESQRSVMYRERAAGMYSVAPYSVAQVVVELPYLVVQTLSYSVIVYWMVAF-IASASKFFYFVFIFGLTLAYFTAYGQMCLNLMPELALANVFTSFFFGFWNLMCGFLIPASAIPGWWIWCYYINPVSWTLYALIVSQLGDLDDEYIVNF 1461          

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|552845019|ref|XP_005851238.1| (hypothetical protein CHLNCDRAFT_137957 [Chlorella variabilis] >gi|307110901|gb|EFN59136.1| hypothetical protein CHLNCDRAFT_137957 [Chlorella variabilis])

HSP 1 Score: 500.745 bits (1288), Expect = 1.551e-146
Identity = 435/1480 (29.39%), Postives = 657/1480 (44.39%), Query Frame = 0

Query:  214 PQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGG--FHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDV---------DEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVL----------------------RKGALVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLNLRTEAVATR---FGALYQSLLTIAFQTAACIPAYFV---RRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPN-------------FYTWVMFLNPLFYGFQGLAVNEFIAKNYGALLPGST-ETIGETVLGQKGLFTNARWVWLGLLANLGITLFYLCLTLVAMMYLEWLQPVSSADTTPEEAIAQSSLALRGPGLLRRQTTERHLELDREESDVDEDGY-SWTSESVQSWGVESSTNTITAFRSFSGLGGGAEDDEDASSTNTSERGGARQGVMGSSVDEETKEAEGLAKDDARGVKFSGQVAEHTYGKETVRPKNENLAHSVLPRAVSSVKLLADLRNGKSSTWQLEAVKAIPFVPTALTFENLWYSVPVNGNE---------------TVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLPKEVSASKCRHFVGDVLTLLELDPIANAVVGPPG-AGLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFRQ--TQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGNDNQGSIGFFSIKNKQFEELTRWEFVKDFFGTFALEHRFRNIGVLIAF 1621
            P    +++G P  GKT+ L+ +AG + R T  + SG      +E    G  F    +      VD++   LTVRET   S          +   + Y +A++E L           D      +RA    G+  +         E ++ LLGL  CADT++G+A  RG+SGG+++RVT  +   GE +                  I+R  +     ++   ++ L QP PE   LFD V++L                      + GA+ Y GP +    +F G+GF CPPR  VADFL  V  P                 S     W           R   P RH+      +  KK       ES  +       A  R     K+  T+       F R   L  R++ + + R  Q + +  +   +F       V      FG ++ S+L   +Q    IP   +   R  VF+KQ++  F+    + + +     P   L+A L+  LVYW  GF+     L   +FL ++  VG      F+L   +T N T A A   + +FFLL+F    IS+   P               + WV          + LA+NEF A ++    P +   T+G  VL  +G  T   W W  +   L      L L +  M ++    P      TPE        AL+   L R++           E D+ E G  +W S + Q  G  S+  +     + S    G E    A +   +E G  R  V  + +   T  A              G+ +    G+E + P++  L  S   + +S     A++   ++         AIPF  TA+TF ++ YSVP+  +                 + LL+G+ G   P  + ALMG+SGAGK+TLLD LAGRKT G I G+I VNG P     F  ++ YVEQ     P  TVAEA  F+A +RLP  V       FV + + L+ELD + +A VG PG +GLS E+ KR+T+  E+V+NPS++F+DEPT+GLDARAA +V+  +R    TGR ++CTIHQPS +IF  FD LLLLK GG TV+ GPLG DS  LI YF+ IPG   L P  NPA WMLE    G      V   ++   +  S L       +    +  + A   + + L     + FG+Q LV LRR +    R PEY + R   + ++G   G +++RQ   + TVAG+++ M +LF +T  +   N   V  ++A ++T F REH   MY+V  + L+  L E+P++++Q L YS   Y    +     +  WF+   F+ L + T  G A   + PSV +A +L +     ++++SG +I    +PG W +   + P+     G+VV+Q G+ +  +I   S   +        +F+ D   TF  E   + + V I F
Sbjct:  192 PGDFTILLGPPGSGKTTFLRTLAGLNRRHTSLKASGQPAVQAQELSYNGRGFDEFVVERSAAYVDDHYGELTVRETFDLSA---------RFQSSGYKKAVLEELAAKERELCISPDPEVDAYMRATAVAGKGNL-------MVEVIIRLLGLDICADTVVGNAMLRGISGGQKKRVTTGKA--GERAQAW----------RVLLGIMRAFKNVCHLYKATLVVGLLQPQPETFDLFDTVILLASGKARGGREMGAWQELGTDWRQPGAVCYHGPREGVLPFFGGIGFVCPPRRGVADFLQQVATP-----------------SDQHKYW---------DMRNQRPYRHVSVLMIENAFKKTELWQGVESQLAQPFDASSADPRALATTKYGQTYSHLLRTNFRRMILLQTRNKIFTIIRTSQVLLMAFVVSTLFWREDKGTVEDGNLFFGVIFYSIL---YQLLGAIPEMHLLVGRLSVFFKQRDVNFYPGWCFAIPTFLMRVPWSFLEATLWTNLVYWLVGFSPSVRFLMLQLFLINIWSVG-----LFQLIAAVTRNDTIATA---VGSFFLLIF----ISLTGAPPRCRAGARMLCLLLLFAWVT---------RALAINEFTAAHWMRPNPSNPGSTLGIDVLQFRGFPTEYWWTWASVGFVLASLALLLLLFIATMTFIG--APRQRRTITPE--------ALQDFQLSRKELLTPQPSF--AEQDMAEQGMVAWPSTATQ--GTSSTNKSGRLAAADSATAPGTEPLAGAPAGPAAEDGAVR--VTVTPLGGPTGAA--------------GRSSSFEAGEEPISPRHLYLMRS--SQRMSQASQQAEVYRQRT---------AIPFDFTAITFRDVEYSVPLPPDADPQRADVPASGPHQGALRLLRGIHGVFRPHVLTALMGASGAGKSTLLDCLAGRKTSGLITGDIRVNGFPKDQHTFARVAGYVEQTDVHMPQTTVAEACHFSARVRLPTSVEKGSREAFVEEAMALVELDRLRHAHVGVPGVSGLSVEQRKRLTLAVELVSNPSVVFMDEPTSGLDARAAGVVMDAVRATVDTGRTVVCTIHQPSADIFEAFDELLLLKPGGSTVYFGPLGDDSQALIRYFQGIPGVRPLPPNYNPANWMLEVTSPGAEEAPGV---DFAQLYAKSDLARQMDGVISQHHEPKAGAAPPLFSELHA---SGFGEQFLVNLRRNFTIYNRSPEYNLTRAAVTTLIGFSFGGMFWRQGDNRSTVAGVLNIMGVLFSSTLFLGISNCLTVQHLIAAQRTVFYREHAAGMYRVAPFALAQQLVELPYLVVQALAYSCIVYWMVWFARDAAKFFWFYFLFFLTLWYFTTLGMAAVNLTPSVPLANVLCSFFFGFWNLLSGFLIPIPAMPGYWVWAAWINPVMWSIYGMVVSQLGSFSNETITNLSGVTETIP-----QFLSD---TFQYETYMQGVIVAILF 1538          

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|585102598|gb|EWM22171.1| (atp-binding cassette superfamily [Nannochloropsis gaditana])

HSP 1 Score: 424.091 bits (1089), Expect = 1.847e-119
Identity = 242/604 (40.07%), Postives = 361/604 (59.77%), Query Frame = 0

Query: 1030 AIPFVPTALTFENLWYSVPV-----NGNETVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLP-KEVSASKCRHFVGDVLTLLELDPIANAVVGP-PGAGLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGT-PALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMA--SLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFRQTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPW-RIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGNDNQGSIGFFSIKNKQFEELTRWEFVKDFF-GTFALEHRFRNIGVLIAF 1621
            ++ F P  L F+++WYSV +      G E V+L+KGV+G   PG + ALMGSSGAGKTTLLDVLAGRKT G I GEILVNG P     F  +  YVEQ    SP+ TV EAL F+A LRLP  +V+A++   FV D+L LLEL  IA+ V+G   G+GL   E KRVTIG E+VANPS+LFLDEPTTGLDA  AF V+R ++K+A +GR+++CTIHQPS  IF  FD LLLL+ GG+TV+ GPLG  S  LI+Y  ++PG  P    G NPA WMLEC+GA  GI  A    ++   +   AL   N E   +L++ P D+    +   +  +R  A    QL  C+ +A ++ WR P Y   R+  S ++ ++ GS++  +  DT   +V  + L++L+T+ V  +N  +V+ V+A E+ +F RE  + MY V AY +S+ L E+P++ + T L+   FY        P+ +  ++++F  +Y++     GQ +  + P+ Q AQ+    +  I ++  G +  P  +   WKF++ L P + + EGLV++Q+  D+      + ++    +     +++ D F G F    ++++IGVL+ +
Sbjct:  960 SLTFQPITLVFKHIWYSVELPKPQGGGKERVELVKGVTGYARPGSLTALMGSSGAGKTTLLDVLAGRKTTGCIIGEILVNGFPKEQRAFSRVMGYVEQTDVHSPHSTVREALLFSATLRLPYTQVTAAQREVFVEDMLALLELSGIADRVIGEDAGSGLLMGERKRVTIGVELVANPSVLFLDEPTTGLDAAKAFEVMRSVKKIAASGRSVLCTIHQPSCAIFEMFDMLLLLRHGGRTVYFGPLGKRSKDLINYLEAVPGVIPLRTGGVNPANWMLECIGA--GIEPAAQPLDFAEYYRDHALARRNEEICDSLSR-PFDSHGHGLEPIAFDSRYAAPLKVQLRACMAKAIKNYWRSPNYNFTRMFISVLVAVVFGSVFHDKPYDTETDIVGRVGLMYLSTSFVGIVNMMSVMPVMAKERAAFYREQASSMYSVFAYGVSYGLVELPYIFVSTGLFINVFYWFIGLAAEPFSKFVYYWVFFALYIVCLVFIGQFLICLLPNQQTAQVAGASIAAIMNLFGGYLCTPRTITPFWKFVYYLVPSHYMLEGLVMSQFEGDSTPVQPIYGLQATPAD-----QYIYDHFGGEFTYGAKWKDIGVLLLY 1555          

HSP 2 Score: 336.265 bits (861), Expect = 2.789e-90
Identity = 243/779 (31.19%), Postives = 367/779 (47.11%), Query Frame = 0

Query:  155 STSVQIQDLSLSYMVEQSNKRFLNVGEALLN----PFLALLRSGGKKQTFQLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACI-AP-----VPTRKLLQTDYGRALVESL-DVDEATKKELRACRGVGRPKI-----RYALARQ--KAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGALVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLNLRTEAVATRFGALYQSLLTIAFQTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHF--FVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEFIAKNYG---------------------ALL-------PGSTETIGETVLGQKGLFTNARWVWLGLLANLGITLFYLCLTLVAMMYLEWL----QPVSSADTTPEEAIAQSSLALRGPGLLRRQTTE 881
            +T V+  DL+ S   + ++   L VG   L     PF  LLR+  ++Q      L+ +     P ++ L++G P  GK+S LKA+AGR  R     L G V YNG+ A S  F +  +  FV + D +   LTV+ET+ F+  C+  P     +P+R        R L + L    EA          +  P       RY   ++  K E  + +LGL  C  TI+G+   RG+SGGE++R+T AE+  G+ +V  +D IS GLDSAA FDI +      +A Q   ++ L QP PEV  LFD V+++  G +VYQGP +    +FE LGFR P R DVADFLV VT  A   F     +    GI       P ST++FV+ F+++E   H+       P                      A W   ++ +F N W  S  +C  R+ ++T RDR ++  RI+Q + +GA+ G +F  L     + +   L+ SL+ I     A IP    +R VFYKQ++AGFF  SS ++A      P+  ++ ++F +L Y+ +  +R  +G  +  +V +A  T +G   +  F+L   L  +L  A     +     ++FSG  I  +D+P ++TW+ ++NPL +G + LAVNEF +  Y                      ALL       P    + GE  L   G  T   W+  G+L  L +    L LT++AM  + W      PV SA    E A  +       PG   +   E
Sbjct:  168 ATEVRFDDLNYSVQQQSTSGTTLTVGAQCLKMWSWPFTCLLRNNQQRQQASFHILKDLRGVLLPGRLTLLLGPPGSGKSSFLKALAGRVKRTRTSVLKGRVTYNGDTAESHRFSLPKVVAFVSQDDCHASTLTVQETVQFAYDCMNGPQAKIGLPSRLRNPLPSHRELAKHLLQEQEAASSSSAPSASICAPSSTLGSERYGANKRNGKVEQTLRVLGLSRCQHTIVGNTLCRGISGGEKKRLTTAEMTGGQVNVLLMDSISTGLDSAATFDICKTLSGCTRALQANIVVCLLQPPPEVFDLFDDVILMDAGHIVYQGPRELILDHFENLGFRRPLRKDVADFLVEVTTSAGAEFIIPAAQRYAAGIPDP----PTSTEEFVRAFQETEIYEHMIDNLERPPSF--------------------APWLRRQRCEFSNGWFRSTWLCLQRQARITLRDRVFLRGRIMQVLLMGAMAGTLFYQLDAHDWSDKVCMLFFSLMFITLGNLATIPTVMEQRAVFYKQRDAGFFPTSSAVVAQMLVQIPIQFVETMVFTSLAYFLSALSRADYGAFYLTYVLVAFSTALGIGQI--FRLVVHLVPSLAQAQPICSLFVLLFVVFSGLTIKGEDIPFYWTWLYWINPLAWGLRALAVNEFSSPTYSQHIIYPPPVPRAIPCDPRRPEALLQYTGSNGPFQCLSEGEIYLINLGFKTKRDWIVYGVLFLLALWSVMLMLTMLAMRLIRWTGQGAAPVPSAAKREELAATED----ENPGYKEKDLNE 916          

HSP 3 Score: 140.198 bits (352), Expect = 2.009e-29
Identity = 163/680 (23.97%), Postives = 282/680 (41.47%), Query Frame = 0

Query:  194 GGKKQTFQLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGA-LVYQGP----PDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQ--EATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLN--LRTEA-VATRFGALYQSLLTIAF-QTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHG--LHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEF------IAKNYGALLPGSTETIGETVLGQKGLFT-NARWVWLGLLANLGITLFYLCL----TLVAMMYLEWLQ 849
            GG K+  +L  ++ +T    P  +  ++G    GKT+LL  +AGR   G    + G +L NG       F  + +  +VE+ D + P  TVRE L+FS            L+  Y +          A ++E+                    E ++ LL L G AD +IG+ +  G+  GER+RVT+   L+   SV  LDE + GLD+A AF+++R  +  + A     +  ++QP+  +  +FD +L+LR G   VY GP      +   Y E +    P RT       G   PA       + E    GI PA         DF + +R     R   +  ++   P       ++  + DS     L+   R    +   N W                R  +Y  +R+   + +  + G VF +    TE  +  R G +Y S   +      + +P     R  FY+++ +  +S  +Y ++      P + +   LF  + YW  G A +     ++++VF A L +V    +  F +   L  N  +A  A   +   + LF G++ + + +  F+ +V +L P  Y  +GL +++F      +   YG     + + I +   G+   FT  A+W       ++G+ L Y+ L    T V M ++  + 
Sbjct:  984 GGGKERVEL--VKGVTGYARPGSLTALMGSSGAGKTTLLDVLAGRKTTGC---IIGEILVNGFPKEQRAF--SRVMGYVEQTDVHSPHSTVREALLFSAT----------LRLPYTQV--------TAAQREVFV------------------EDMLALLELSGIADRVIGEDAGSGLLMGERKRVTIGVELVANPSVLFLDEPTTGLDAAKAFEVMRSVK-KIAASGRSVLCTIHQPSCAIFEMFDMLLLLRHGGRTVYFGPLGKRSKDLINYLEAVPGVIPLRT-------GGVNPA-----NWMLECIGAGIEPA-----AQPLDFAEYYRDHALARRNEEICDSLSRPFDSHGHGLEPIAFDSRYAAPLKVQLRACMAKAIKNYW----------------RSPNYNFTRMFISVLVAVVFGSVFHDKPYDTETDIVGRVGLMYLSTSFVGIVNMMSVMPVMAKERAAFYREQASSMYSVFAYGVSYGLVELPYIFVSTGLFINVFYWFIGLAAEPFSKFVYYWVFFA-LYIVCLVFIGQFLIC--LLPNQQTAQVAGASIAAIMNLFGGYLCTPRTITPFWKFVYYLVPSHYMLEGLVMSQFEGDSTPVQPIYGLQATPADQYIYDHFGGE---FTYGAKW------KDIGVLLLYISLLRIGTFVVMTFVRHIN 1574          

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|113533642|dbj|BAF06025.1| (Os01g0724500, partial [Oryza sativa Japonica Group])

HSP 1 Score: 400.593 bits (1028), Expect = 1.228e-118
Identity = 226/583 (38.77%), Postives = 341/583 (58.49%), Query Frame = 0

Query: 1030 AIPFVPTALTFENLWYSV--PVNGNETVD---LLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLPKEVSASKCRHFVGDVLTLLELDPIANAVVGPPGA-GLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFR--QTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGNDNQGSIGFFSIKNKQFEELTRWEFVKDFFG 1604
             +PF P  ++F  + Y V  P++   T D   LL G+SG+  PG + ALMG SGAGKTTL+DVL+GRKT G I GEI ++G P +   F  IS Y EQ    SP +TV E+L F+A LRLPKEV+  + + FV +V+ L+EL  + +A+VG PG  GLS E+ KR+TI  E+VANPSI+F+DEPT+GLDARAA +V+R +R    TGR ++CTIHQPS +IF  FD LLLLKRGGQ ++ GPLG +S  +++YF +IPG P ++   NPATWML+   A + +   +    Y   + +S +       ++ L+  P  +      S  ++   S   Q  +CL + W + WR P+Y + R+  +    L+LG++++R     ++   L+  +  ++ A   V   NS  V  VVA+E+T F RE    MY    Y L+  + EIP+V ++T++Y+   YP  ++   P +  WFF  SF   L+ T +G    +V P++QVA IL      +F++ SG  I    +P  W + + L P+     GL+V+Q+G+       F ++  +  +++    F+KD+FG
Sbjct:   92 VLPFEPLYMSFNEINYYVDMPLSQGVTADKLQLLSGISGAFRPGVLTALMGVSGAGKTTLMDVLSGRKTGGYIEGEIYISGYPKNQATFARISGYCEQNDIHSPQITVRESLLFSAFLRLPKEVNDQEKKIFVDEVMELVELTGLKDAIVGLPGVNGLSTEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVNTGRTVVCTIHQPSIDIFEAFDELLLLKRGGQVIYSGPLGTNSHKVVEYFEAIPGVPKIEENRNPATWMLDVSSAASEVRLEIDFAEY---YRSSTMHQRTKALVKELSNPPPGSDDLYFPSQYSQ---STFNQFKLCLWKQWWTYWRSPDYNLVRIFFALFTALMLGTIFWRVGHKMESSKDLLVIIGSMYAAVLFVGFENSVTVQPVVAVERTVFYRERAAGMYSAIPYALAQVVVEIPYVFVETVIYTLIVYPMMSFQWTPAKFFWFFYVSFFTFLYFTYYGMMNVSVSPNLQVASILGAAFYTLFNLFSGFFIPRPKIPKWWVWYYWLCPVAWTVYGLIVSQYGDVED----FITVPGQSDQQVR--PFIKDYFG 662          

HSP 2 Score: 145.976 bits (367), Expect = 5.027e-32
Identity = 137/611 (22.42%), Postives = 263/611 (43.04%), Query Frame = 0

Query:  201 QLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGA-LVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKS---EPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLNLRTEAVATR-----FGALYQSLLTIAFQTAACI-PAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEFIAKNYGALLPGSTE 801
            +L+ L  I+ AF P  +  ++G    GKT+L+  ++GR    TG  + G +  +G       F   S  C  E+ D + P +TVRE+L+FS     P                   +V++  KK                      + VM L+ L G  D I+G     G+S  +R+R+T+A  L+   S+  +DE ++GLD+ AA  ++R  R +V   +   +  ++QP+ ++   FD++L+L++G  ++Y GP          LG       +  + + GV  P  +  R     + +   + ++V+      DF + +R S   +  + L +E +  P    +    ++ S S   +                     F +C  +++   +R   Y + RI   +F   + G +F  +  +  +++      G++Y ++L + F+ +  + P   V R VFY+++ AG +S   Y LA      P V ++ V++  +VY    F        +F +++  T + +       ++  ++ NL  A+           LFSGF I    +P ++ W  +L P+ +   GL V+++        +PG ++
Sbjct:  121 KLQLLSGISGAFRPGVLTALMGVSGAGKTTLMDVLSGRK---TGGYIEGEIYISGYPKNQATFARISGYC--EQNDIHSPQITVRESLLFSAFLRLP------------------KEVNDQEKKIF-------------------VDEVMELVELTGLKDAIVGLPGVNGLSTEQRKRLTIAVELVANPSIIFMDEPTSGLDARAAAIVMRTVRNTVNTGR-TVVCTIHQPSIDIFEAFDELLLLKRGGQVIYSGP----------LGTNSHKVVEYFEAIPGV--PKIEENRNPATWMLDVSSAASEVRL---EIDFAEYYRSSTMHQRTKALVKELSNPPPGSDDLYFPSQYSQSTFNQ---------------------FKLCLWKQWWTYWRSPDYNLVRIFFALFTALMLGTIFWRVGHKMESSKDLLVIIGSMYAAVLFVGFENSVTVQPVVAVERTVFYRERAAGMYSAIPYALAQVVVEIPYVFVETVIYTLIVYPMMSFQWTPAKFFWFFYVSFFTFLYFTYYGMMNVS--VSPNLQVASILGAAFYTLFNLFSGFFIPRPKIPKWWVWYYWLCPVAWTVYGLIVSQYGDVEDFITVPGQSD 650          

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|1142680506|gb|ONM38549.1| (ABC transporter G family member 29 [Zea mays] >gi|1142680507|gb|ONM38550.1| ABC transporter G family member 29 [Zea mays] >gi|1142680514|gb|ONM38557.1| ABC transporter G family member 29 [Zea mays])

HSP 1 Score: 399.053 bits (1024), Expect = 1.744e-118
Identity = 212/585 (36.24%), Postives = 338/585 (57.78%), Query Frame = 0

Query: 1031 IPFVPTALTFENLWYSVPVNGN--------ETVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLPKEVSASKCRHFVGDVLTLLELDPIANAVVGPPGA-GLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFR--QTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGNDNQGSIGFFSIKNKQFEELTRWEFVKDFFG 1604
            +PF P +++F  + Y V +           + + LL G+SG+  PG + ALMG SG+GKTTL+DVL+GRKT G I GEI ++G P + + F  IS Y EQ    SP +T+ E+L F+A +RLPKEV+  + + FV +V+ L+EL+ + +A+VG PG  GLS E+ KR+T+  E+VANPSI+F+DEPT+GLDARAA +V+R +R    TGR ++CTIHQPS +IF  FD LLL+KRGGQ ++ GPLG +S  +++YF ++PG P +  G NPATWML+   A T +   +   ++   +++S +   N   ++ L++ P  +         T+   S   Q   CL + W + WR P+Y + R+  +    L+LG +++R      + + L+  +  ++ A   +   N      V+A+E+T F RE    MY    Y  S  + EIP+V +++++Y+   YP  ++     +  WFF  SF+  L+ T +G    A+ P+ QVA I       +F++ SG ++    +P  W + + + P+     GL+V+Q+G D +  I      ++Q +      F+KD+FG
Sbjct:   58 LPFEPLSMSFNEINYYVDMPAEMKSQGVTADKLQLLSGISGAFRPGVLTALMGVSGSGKTTLMDVLSGRKTGGYIEGEIYISGYPKNQETFARISGYCEQNDIHSPQITIRESLLFSAFMRLPKEVTDQEKKIFVDEVMELVELNGLKDAIVGLPGVNGLSTEQRKRLTVAVELVANPSIIFMDEPTSGLDARAAAVVMRTVRNTVNTGRTVVCTIHQPSIDIFEAFDELLLMKRGGQIIYSGPLGRNSHKVVEYFEAVPGIPKIKEGCNPATWMLDVTSASTEVQLNI---DFAEHYKSSTMHQRNKALVKELSKPPPGSSDLY---FPTQYSQSTFDQFRFCLWKQWLTYWRSPDYNLVRMFFALFTALLLGIIFWRVGSKMKSSSDLLIIVGSMYFAVAFIGFENCITAQPVIAVERTVFYRERAAGMYSAIPYAFSQVVAEIPYVFVESVIYTVIVYPMMSFQWTLAKFFWFFYISFLSFLYFTYYGMMGVAITPNPQVASIFAASFYTLFNLFSGFIVPRSRIPVWWIWYYWICPVAWTVYGLIVSQYG-DVEDLIKVPGKPDQQVK-----AFIKDYFG 630          

HSP 2 Score: 146.747 bits (369), Expect = 2.067e-32
Identity = 138/600 (23.00%), Postives = 258/600 (43.00%), Query Frame = 0

Query:  201 QLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQVLVLRKGA-LVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTG-PAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKS---EPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLNLRTEAVATR-----FGALYQSLLTIAFQTAACI---PAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEF 787
            +L+ L  I+ AF P  +  ++G    GKT+L+  ++GR    TG  + G +  +G       F   S  C  E+ D + P +T+RE+L+FS     P                      E T +E +                   + VM L+ L G  D I+G     G+S  +R+R+T+A  L+   S+  +DE ++GLD+ AA  ++R  R +V   +   +  ++QP+ ++   FD++L++++G  ++Y GP          LG        V ++   V G P  K        + +   +  +VQ      DF + ++ S   +  + L +E +  P   ++    T+ S S   +     W++         W+T             +R   Y + R+   +F   L G++F  + ++  ++       G++Y ++  I F+   CI   P   V R VFY+++ AG +S   Y  +   A  P V +++V++  +VY    F        +F +++ L+ + +       +       + S  AAS    F   LFSGFI+    +P ++ W  ++ P+ +   GL V+++
Sbjct:   89 KLQLLSGISGAFRPGVLTALMGVSGSGKTTLMDVLSGRK---TGGYIEGEIYISGYPKNQETFARISGYC--EQNDIHSPQITIRESLLFSAFMRLP---------------------KEVTDQEKKIF----------------VDEVMELVELNGLKDAIVGLPGVNGLSTEQRKRLTVAVELVANPSIIFMDEPTSGLDARAAAVVMRTVRNTVNTGR-TVVCTIHQPSIDIFEAFDELLLMKRGGQIIYSGP----------LGRNS---HKVVEYFEAVPGIPKIKEGCNPATWMLDVTSASTEVQL---NIDFAEHYKSSTMHQRNKALVKELSKPPPGSSDLYFPTQYSQSTFDQFRFCLWKQ---------WLT------------YWRSPDYNLVRMFFALFTALLLGIIFWRVGSKMKSSSDLLIIVGSMYFAVAFIGFEN--CITAQPVIAVERTVFYRERAAGMYSAIPYAFSQVVAEIPYVFVESVIYTVIVYPMMSFQWTLAKFFWFFYISFLSFLYFTYYGMMGVAITPNPQVASIFAASFYTLF--NLFSGFIVPRSRIPVWWIWYYWICPVAWTVYGLIVSQY 604          

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|298708503|emb|CBJ30625.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 395.586 bits (1015), Expect = 3.951e-118
Identity = 228/582 (39.18%), Postives = 341/582 (58.59%), Query Frame = 0

Query: 1045 YSVP-VNGNETVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRL-PKEVSASKCRHFVGDVLTLLELDPIANAVVGPPGAG-LSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAFLVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGTPALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVMASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFRQTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPWRIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQW-GNDNQGSIGFFSIKNKQFEELTRWEFVKDFF-GTFALEHRFRNIGVLIAF 1621
            YSVP  +G   + LL  +SG   PG M ALMGSSGAGKTTLLDVLAGRKT G+I G+I VNG P   + F  I+ YVEQ    S  +TV EAL F+A +RL   ++ A  C  FVG +L++LEL+ IA+ ++G   +G LS E+ KR T+G E+ ANPS++ LDEPT+GLDAR+A +V+R +RK+A TGRA+ICTIHQPS  +F  FDSLLLLK+GGQTVF G LGA+S  LI Y  S+P TP++    NPATWMLEC+GAGT     V    Y   ++ S L +  +  L TL   P+ ++    +S+    R+    Q+  C+ RA    WR P Y  +R++ + V+ +I G+    +  ++ A + +   +++++T  V +I     +    LE+  F RE    MY   AY + + + E+P++++ TL +   FY          +  +++++  +++ F    G     + PS   A +L   LV +FS+ +G +I P  +PG W + + L PL+ + EG+V TQ+ GND        +I+      +T  E+V  +F G +   +R+ ++  L+ F
Sbjct:    3 YSVPHPSGEGELTLLDEISGFCKPGEMTALMGSSGAGKTTLLDVLAGRKTGGTITGDICVNGHPKRQETFIRIAGYVEQQDMHSAVVTVKEALMFSATMRLESSKMDADGCEKFVGGILSVLELEEIADRLIGSEASGGLSLEQRKRTTLGVELAANPSLVLLDEPTSGLDARSAQVVMRAIRKVAATGRAVICTIHQPSTYLFEMFDSLLLLKKGGQTVFFGELGAESSKLISYLLSVPNTPSIRDNVNPATWMLECIGAGT--TGKVDPQVYADVYKKSKLKSGTLRELETLMVPPAGSEPLQFSSVYAAPRS---LQIKTCIDRAILQYWRNPNYNWSRIMLALVIAIIFGTASIGRDLESEADVGAQTGVIYMSTMFVGSICMQTAIAAGFLERIVFYREKAANMYSSLAYVIGYTVAEVPYIVVITLAFCCIFYFVMGLAATAHQFFFYWMYFMLWVTFMVFNGMMFVFIIPSFSTAGVLAGTLVSMFSVFAGFLISPAKIPGLWLWAYYLNPLHYILEGMVSTQFNGNDR-------TIETATQGPMTVEEYVDGYFGGEYKYSNRWYDVMALLLF 572          

HSP 2 Score: 137.117 bits (344), Expect = 1.377e-29
Identity = 153/601 (25.46%), Postives = 260/601 (43.26%), Query Frame = 0

Query:  201 QLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFDIVRMARASVQAFQYCSIIALNQPAPEVLALFDQV-LVLRKGALVYQGPPDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDE----LAETGISPAQVQWPGSTKDFVQRFR-KSEPGRHLFQEATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFL--NLRTEA-VATRFGALYQSLL---TIAFQTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHFF--VFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEF 787
            +L  L  I+    P +M  ++G    GKT+LL  +AGR   GT   ++G +  NG       F    +A +VE+ D +  ++TV+E L+FS        T +L                E++K +   C              +    ++++L L+  AD +IG  +  G+S  +R+R TL   L    S+  LDE ++GLD+ +A  ++R  R  V A     I  ++QP+  +  +FD + L+ + G  V          +F  LG      + +  +L+ V  P   S R +++     L   G        P    D  ++ + KS   R L  E  M P   A S     SS     R L+                     C  R     +R+ +Y  SRI+  + +  + G   +  +L +EA V  + G +Y S +   +I  QTA  I A F+ R VFY++K A  +S  +Y++  T A  P +++  + F  + Y+  G A   H   F+   F+  +T + +  M F     F+  + ++A   +G +     +F+GF+IS   +P  + W  +LNPL Y  +G+   +F
Sbjct:   13 ELTLLDEISGFCKPGEMTALMGSSGAGKTTLLDVLAGRKTGGT---ITGDICVNGHPKRQETF--IRIAGYVEQQDMHSAVVTVKEALMFSA-------TMRL----------------ESSKMDADGCE-------------KFVGGILSVLELEEIADRLIGSEASGGLSLEQRKRTTLGVELAANPSLVLLDEPTSGLDARSAQVVMRAIR-KVAATGRAVICTIHQPSTYLFEMFDSLLLLKKGGQTV----------FFGELG---AESSKLISYLLSV--PNTPSIRDNVNPATWMLECIGAGTTGKVDPQVYADVYKKSKLKSGTLREL--ETLMVP--PAGSEPLQFSSVYAAPRSLQIK------------------TCIDRAILQYWRNPNYNWSRIMLALVIAIIFGTASIGRDLESEADVGAQTGVIYMSTMFVGSICMQTA--IAAGFLERIVFYREKAANMYSSLAYVIGYTVAEVPYIVVITLAFCCIFYFVMGLAATAHQFFFYWMYFMLWVTFMVFNGMMF----VFIIPSFSTAGVLAGTLVSMFSVFAGFLISPAKIPGLWLWAYYLNPLHYILEGMVSTQF 528          

BLAST of EWM27219.1 vs. NCBI_GenBank
Match: gi|553189606|ref|XP_005855037.1| (atp-binding cassette superfamily [Nannochloropsis gaditana CCMP526] >gi|422294022|gb|EKU21322.1| atp-binding cassette superfamily [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 406.757 bits (1044), Expect = 5.675e-118
Identity = 242/630 (38.41%), Postives = 361/630 (57.30%), Query Frame = 0

Query: 1030 AIPFVPTALTFENLWYSVPV-----NGNETVDLLKGVSGSVAPGCMMALMGSSGAGKTTLLDVLAGRKTEGSIRGEILVNGVPLSSDVFKTISAYVEQFGCISPYMTVAEALQFNAELRLP-KEVSASKCRHFVGDVLTLLELDPIANAVVGP-PGAGLSFEELKRVTIGCEVVANPSILFLDEPTTGLDARAAF--------------------------LVVRVMRKLAQTGRAIICTIHQPSKEIFNFFDSLLLLKRGGQTVFCGPLGADSIHLIDYFRSIPGT-PALDPGENPATWMLECLGAGTGIGAAVTACNYVAEWETSALCASNIETLRTLTQTPSDAQQAVM--ASLKTRERASFGKQLLVCLRRAWRSLWRMPEYQIARVLGSGVMGLILGSLYFRQTQDTVAGLVSFMALLFLATTCVSNINSTAVLQVVALEKTSFCREHYNRMYQVGAYTLSWFLCEIPWVLLQTLLYSATFYPTCNYNLAPW-RIGWFFIFSFMYLLFSTAFGQAVAAVCPSVQVAQILLNCLVPIFSMMSGMVIIPEDLPGKWKFLWVLTPLNKVFEGLVVTQWGNDNQGSIGFFSIKNKQFEELTRWEFVKDFF-GTFALEHRFRNIGVLIAF 1621
            ++ F P  L F+++WYSV +      G E V+L+KGV+G   PG + ALMGSSGAGKTTLLDVLAGRKT G I GEILVNG P     F  +  YVEQ    SP+ TV EAL F+A LRLP  +V+A++   FV D+L LLEL  IA+ V+G   G+GL   E KRVTIG E+VANPS+LFLDEPTTGLDA  AF                           V+R ++K+A +GR+++CTIHQPS  IF  FD LLLL+ GG+TV+ GPLG  S  LI+Y  ++PG  P    G NPA WMLEC+GA  GI  A    ++   +   AL   N E   +L++ P D+    +   +  +R  A    QL  C+ +A ++ WR P Y   R+  S ++ ++ GS++  +  DT   +V  + L++L+T+ V  +N  +V+ V+A E+ +F RE  + MY V AY +S+ L E+P++ + T L+   FY        P+ +  ++++F  +Y++     GQ +  + P+ Q AQ+    +  I ++  G +  P  +   WKF++ L P + + EGLV++Q+  D+      + ++    +     +++ D F G F    ++++IGVL+ +
Sbjct:  325 SLTFQPITLVFKHIWYSVELPKPQGGGKERVELVKGVTGYARPGSLTALMGSSGAGKTTLLDVLAGRKTTGCIIGEILVNGFPKEQRAFSRVMGYVEQTDVHSPHSTVREALLFSATLRLPYTQVTAAQREVFVEDMLALLELSGIADRVIGEDAGSGLLMGERKRVTIGVELVANPSVLFLDEPTTGLDAAKAFEVGIRSEQPNIFRGDQSEVSAFVSSMSVMRSVKKIAASGRSVLCTIHQPSCAIFEMFDMLLLLRHGGRTVYFGPLGKRSKDLINYLEAVPGVIPLRTGGVNPANWMLECIGA--GIEPAAQPLDFAEYYRDHALARRNEEICDSLSR-PFDSHGHGLEPIAFDSRYAAPLKVQLRACMAKAIKNYWRSPNYNFTRMFISVLVAVVFGSVFHDKPYDTETDIVGRVGLMYLSTSFVGIVNMMSVMPVMAKERAAFYREQASSMYSVFAYGVSYGLVELPYIFVSTGLFINVFYWFIGLAAEPFSKFVYYWVFFALYIVCLVFIGQFLICLLPNQQTAQVAGASIAAIMNLFGGYLCTPRTITPFWKFVYYLVPSHYMLEGLVMSQFEGDSTPVQPIYGLQATPAD-----QYIYDHFGGEFTYGAKWKDIGVLLLY 946          

HSP 2 Score: 128.257 bits (321), Expect = 5.544e-26
Identity = 166/702 (23.65%), Postives = 282/702 (40.17%), Query Frame = 0

Query:  194 GGKKQTFQLKALQHITTAFAPQKMYLVIGGPKCGKTSLLKAIAGRSLRGTGYRLSGSVLYNGEEAGSGGFHMTSLACFVEEVDNNEPLLTVRETLVFSGACIAPVPTRKLLQTDYGRALVESLDVDEATKKELRACRGVGRPKIRYALARQKAETVMTLLGLQGCADTIIGDASRRGVSGGERRRVTLAEVLMGEYSVTCLDEISNGLDSAAAFD---------IVRMARASVQAF----------------QYCSIIALNQPAPEVLALFDQVLVLRKGA-LVYQGP----PDEAKGYFEGLGFRCPPRTDVADFLVGVTGPAFKSFRASLDELAETGISPAQVQWPGSTKDFVQRFRKSEPGRHLFQ--EATMSPHKKANSSIKTESSDSGIRRILEAAWRENEQRKFYNTWMTSFVICFHREFKLTFRDRSYVVSRILQDIFLGALTGLVFLN--LRTEA-VATRFGALYQSLLTIAF-QTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHG--LHFFVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEF------IAKNYGALLPGSTETIGETVLGQKGLFT-NARWVWLGLLANLGITLFYLCL----TLVAMMYLE 846
            GG K+  +L  ++ +T    P  +  ++G    GKT+LL  +AGR   G    + G +L NG       F  + +  +VE+ D + P  TVRE L+FS            L+  Y +          A ++E+                    E ++ LL L G AD +IG+ +  G+  GER+RVT+   L+   SV  LDE + GLD+A AF+         I R  ++ V AF                    +  ++QP+  +  +FD +L+LR G   VY GP      +   Y E +    P RT       G   PA       + E    GI PA         DF + +R     R   +  ++   P       ++  + DS     L+   R    +   N W                R  +Y  +R+   + +  + G VF +    TE  +  R G +Y S   +      + +P     R  FY+++ +  +S  +Y ++      P + +   LF  + YW  G A +     ++++VF A L +V    +  F +   L  N  +A  A   +   + LF G++ + + +  F+ +V +L P  Y  +GL +++F      +   YG     + + I +   G+   FT  A+W       ++G+ L Y+ L    T V M ++ 
Sbjct:  349 GGGKERVEL--VKGVTGYARPGSLTALMGSSGAGKTTLLDVLAGRKTTGC---IIGEILVNGFPKEQRAF--SRVMGYVEQTDVHSPHSTVREALLFSAT----------LRLPYTQV--------TAAQREVFV------------------EDMLALLELSGIADRVIGEDAGSGLLMGERKRVTIGVELVANPSVLFLDEPTTGLDAAKAFEVGIRSEQPNIFRGDQSEVSAFVSSMSVMRSVKKIAASGRSVLCTIHQPSCAIFEMFDMLLLLRHGGRTVYFGPLGKRSKDLINYLEAVPGVIPLRT-------GGVNPA-----NWMLECIGAGIEPA-----AQPLDFAEYYRDHALARRNEEICDSLSRPFDSHGHGLEPIAFDSRYAAPLKVQLRACMAKAIKNYW----------------RSPNYNFTRMFISVLVAVVFGSVFHDKPYDTETDIVGRVGLMYLSTSFVGIVNMMSVMPVMAKERAAFYREQASSMYSVFAYGVSYGLVELPYIFVSTGLFINVFYWFIGLAAEPFSKFVYYWVFFA-LYIVCLVFIGQFLIC--LLPNQQTAQVAGASIAAIMNLFGGYLCTPRTITPFWKFVYYLVPSHYMLEGLVMSQFEGDSTPVQPIYGLQATPADQYIYDHFGGE---FTYGAKW------KDIGVLLLYISLLRIGTFVVMTFVR 962          

HSP 3 Score: 109.383 bits (272), Expect = 3.157e-20
Identity = 82/286 (28.67%), Postives = 128/286 (44.76%), Query Frame = 0

Query:  630 LYQSLLTIAFQTAACIPAYFVRRHVFYKQKNAGFFSCSSYLLASTTASAPLVLLDAVLFGTLVYWCAGFARDQHGLHF--FVFLAHLTMVGWAMMSFFKLTTFLTANLTSAAAASGIVTFFLLLFSGFIISVKDVPNFYTWVMFLNPLFYGFQGLAVNEFIAKNYG---------------------ALL-------PGSTETIGETVLGQKGLFTNARWVWLGLLANLGITLFYLCLTLVAMMYLEWL----QPVSSADTTPEEAIAQSSLALRGPGLLRRQTTE 881
            L+ SL+ I     A IP    +R VFYKQ++AGFF  SS ++A      P+  ++ ++F +L Y+ +  +R  +G  +  +V +A  T +G   +  F+L   L  +L  A     +     ++FSG  I  +D+P ++TW+ ++NPL +G + LAVNEF +  Y                      ALL       P    + GE  L   G  T   W+  G+L  L +    L LT++AM  + W      PV SA    E A  +       PG   +   E
Sbjct:    2 LFFSLMFITLGNLATIPTVMEQRAVFYKQRDAGFFPTSSAVVAQMLVQIPIQFVETMVFTSLAYFLSALSRADYGAFYLTYVLVAFSTALGIGQI--FRLVVHLVPSLAQAQPICSLFVLLFVVFSGLTIKGEDIPFYWTWLYWINPLAWGLRALAVNEFSSPTYSQHIIYPPPVPRAIPCDPRRPEALLQYTGSNGPFQCLSEGEIYLINLGFKTKRDWIVYGVLFLLALWSVMLMLTMLAMRLIRWTGQGAAPVPSAAKREELAATED----ENPGYKEKDLNE 281