EWM27232.1, cds3468 (CDS) Nannochloropsis gaditana

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|585109412|gb|EWM27232.1| (5 -3 exoribonuclease 2 [Nannochloropsis gaditana])

HSP 1 Score: 2462.95 bits (6382), Expect = 0.000e+0
Identity = 1198/1198 (100.00%), Postives = 1198/1198 (100.00%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQRPQVVDGVPLPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPEELDKEWDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDAMQGQKADDGLARGEGSRFPYLKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRRVREVERRHGGTVQELSARIVPADVQPLGRPKLPHPVPRLSPVASPFLPAPPGPSPEEKNMKNKSVAQQLREKLKKPKKELENGVAGVEGGPEASDAPIGDDCEAGMKHELDHTTKMETPVIEASDSAKPVKRVKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLETTASAQDSQEGTKELEEDDEDVGDDAMGEEEGKEEFEDPHIVVPPELEGTELAAAQKELAERVKSKQEARLDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTPHASALLPGLTHPPRRLTDRDLQPRRPPRLGRGINIAELGTQFGFNEVHATAAHRLIVQNMPRGAGSGSMLIYQQNQQQRMSCGGYIGSGQTGTRSWGSFEPQNQGKQRPPPRLPAPSLYPGLGGGLACGAYSATPNLPHPAHMGPRPPLLFPEPPMMSGGPPFPPGPYSHQVAPHYSGQKPQSQQQQQQQQQQQQYGQHAYQVQQASGFPPTPLFQGIDAGLMNSLRRGVAHAVEQRSHEGRGGQRLHPPRPLHSKNGQQHQRR 1198
            MGVPAFFRWLSEKYPRTVLDMVEQRPQVVDGVPLPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPEELDKEWDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDAMQGQKADDGLARGEGSRFPYLKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRRVREVERRHGGTVQELSARIVPADVQPLGRPKLPHPVPRLSPVASPFLPAPPGPSPEEKNMKNKSVAQQLREKLKKPKKELENGVAGVEGGPEASDAPIGDDCEAGMKHELDHTTKMETPVIEASDSAKPVKRVKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLETTASAQDSQEGTKELEEDDEDVGDDAMGEEEGKEEFEDPHIVVPPELEGTELAAAQKELAERVKSKQEARLDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTPHASALLPGLTHPPRRLTDRDLQPRRPPRLGRGINIAELGTQFGFNEVHATAAHRLIVQNMPRGAGSGSMLIYQQNQQQRMSCGGYIGSGQTGTRSWGSFEPQNQGKQRPPPRLPAPSLYPGLGGGLACGAYSATPNLPHPAHMGPRPPLLFPEPPMMSGGPPFPPGPYSHQVAPHYSGQKPQSQQQQQQQQQQQQYGQHAYQVQQASGFPPTPLFQGIDAGLMNSLRRGVAHAVEQRSHEGRGGQRLHPPRPLHSKNGQQHQRR
Sbjct:    1 MGVPAFFRWLSEKYPRTVLDMVEQRPQVVDGVPLPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPEELDKEWDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDAMQGQKADDGLARGEGSRFPYLKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRRVREVERRHGGTVQELSARIVPADVQPLGRPKLPHPVPRLSPVASPFLPAPPGPSPEEKNMKNKSVAQQLREKLKKPKKELENGVAGVEGGPEASDAPIGDDCEAGMKHELDHTTKMETPVIEASDSAKPVKRVKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLETTASAQDSQEGTKELEEDDEDVGDDAMGEEEGKEEFEDPHIVVPPELEGTELAAAQKELAERVKSKQEARLDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTPHASALLPGLTHPPRRLTDRDLQPRRPPRLGRGINIAELGTQFGFNEVHATAAHRLIVQNMPRGAGSGSMLIYQQNQQQRMSCGGYIGSGQTGTRSWGSFEPQNQGKQRPPPRLPAPSLYPGLGGGLACGAYSATPNLPHPAHMGPRPPLLFPEPPMMSGGPPFPPGPYSHQVAPHYSGQKPQSQQQQQQQQQQQQYGQHAYQVQQASGFPPTPLFQGIDAGLMNSLRRGVAHAVEQRSHEGRGGQRLHPPRPLHSKNGQQHQRR 1198          

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|1210511899|dbj|GAX29139.1| (5'-3' exoribonuclease 2 [Fistulifera solaris])

HSP 1 Score: 760.37 bits (1962), Expect = 0.000e+0
Identity = 453/1071 (42.30%), Postives = 620/1071 (57.89%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQR--PQVVDGVPL-PMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPEELDKEWDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDAMQGQKADDGLARGE--GSRFPYLKPFQMLHIPTLREYLEEEFRSL---RGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRRVREVERRHGGTVQELSARIVPADVQPLGRPKLPHPVPRLSPVASPFLPAPPGPSPEEKNMKNKSVAQQLREKLKKPKKELENGVAGVEGGPEASDAPIGDDCEAGMKHEL-DHTTKMETPVIEASDSAKPVKRVKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLETTASAQDSQEGTKELEEDDEDVGDDAMGEEEGKEEFEDPHIVVPPELEGTELAAAQKELAERVKSK----QEARLDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHV---AFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVG--GSLLCLHEYHPTFRAIARVLEVEGAQDRAG----VEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDV-------------EDNRVLVAGFTLPSQTTPHASALLPGLTHPPRRLTDRDLQPRRPPRLGR-GINIAELGTQFGFNEVHATAAHRLIVQNMPRGAGSGSMLIYQQNQQQRMSCGGYIGSGQTGTRSWGSFEP 1035
            MGVPAF+RWLSEKYP+ V D++E+R   Q    VPL P++++ PNPN +E DNLYIDMNGIIHPCSHPE+   P TE EMF NV  YVDR+  A+RPR+L++LAIDGVAPRAKMNQQR RRFRSA +ARE HE++   R+E+V  G  VPP +  K WDSNVITPGT FM+ LA +LRFY+  R++T + W+ ++V+FSD S PGEGEHKIM  IR +RAQ GY+PNT H+LHGLDADLIMLALATHE HF I RE V FGR+ ++  +    +S F  +  QKA D     E         KP Q + IP LREYL  EF  +   + LPF  + ER++DDFVFLCFFVGNDFLPHLPSLDIRDGA+D+LLNVYKR+LP+MG Y+T  GG VNL  VDIILAEVG +ED VF  +   E++ +    RR  +  R+      +      P +V P+   ++     R+                 E+  K+K +A     + K+  + ++     +    E  +A   +D +A +   + + + K+E  + ++    +    VK E+        + +++KE    T+LG   +  LE      + +    +++ +   VGD     +    + E+   +  P++E   +     ++  R K+K    Q+ +LD   + + D +R HEAGWKDRYY DK K  ++E  GGREH++R+Y+ GLCWV +YYY GC +W W+YP+HY PFASDL NIER+     AFE   P  P+EQLMAVLP +S  A+P     LM   +SPI DFYP+++ VDPNGKAMPWLWV LLPFIDE RLL +++      ++ E   NV G     L +H  HP  + +A +L+ +G   +AG     +       GFSG++ PP  +    +   V  P  PPS    +             E N  +   F+ P + + H S +LPG       LTD D + RR PRL R G  IA +G   G              Q+   G GS ++  Y++   ++   G  +   Q GTR+WG+ EP
Sbjct:    1 MGVPAFYRWLSEKYPKIVQDILEERVPNQPNSNVPLLPLNIQGPNPNGIECDNLYIDMNGIIHPCSHPENGLQPTTEEEMFENVCHYVDRLVRAIRPRKLLYLAIDGVAPRAKMNQQRARRFRSAQEARELHELEEHVREELVKSGQFVPPHK--KTWDSNVITPGTAFMLHLAEFLRFYIRKRLSTDRNWKDLRVIFSDASLPGEGEHKIMSHIRLQRAQAGYNPNTVHMLHGLDADLIMLALATHEAHFYITRELVLFGRKSQEQHEQRQMESGF--LDAQKALDEKVGPEAMAMEINQQKPLQRISIPILREYLAAEFAVVLRPQALPFAASLERIIDDFVFLCFFVGNDFLPHLPSLDIRDGALDYLLNVYKRLLPSMGGYITNSGGNVNLSNVDIILAEVGAIEDYVFGMKHENEKREEE---RRREQSMRKKNNLRND-----APPEVAPVEHHRVKGRAARIM----------------ERQNKDKDIALSRGHQAKEDARHVQKAKRMM----EQDNAKAAEDLKASLLGLVSEKSVKIENGIADSLPKHEDADNVKIEEDQ------EHISVKEE---TSLG--TKRSLEEVEKNSEDEPPPAKVKSEPGVVGDMEDEFDADDADEEEDLDIEKPKIEDL-IEDTDPDVTSRFKAKVKEEQQKKLDEHAKNVVDNVRLHEAGWKDRYYSDKCKADDIESNGGREHLFRSYVMGLCWVMKYYYDGCPSWKWYYPYHYGPFASDLKNIERFEKDCRAFELSTPFNPVEQLMAVLPSDSKHAIPKAARWLMEDPESPIIDFYPKEILVDPNGKAMPWLWVVLLPFIDEDRLLAALSPTMQNWTKQELFANVRGLDDGYLYVHRSHPLSQKLATILK-DGNTAKAGKTRLTDTAMYGCAGFSGSVRPPLSNELYPVEEDVTIP--PPSSASKIVRSSDDNIFSEPLEPNDAVCVSFSEPPKLS-HKSIILPGSKLLNPVLTDEDKRIRR-PRLNRFGGTIANMGVGNG--------------QSYQSGYGSMNLGSYERQLAEQTGRGHQM--HQAGTRAWGAMEP 1006          

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|358055903|dbj|GAA98248.1| (hypothetical protein E5Q_04931 [Mixia osmundae IAM 14324])

HSP 1 Score: 639.417 bits (1648), Expect = 0.000e+0
Identity = 408/1059 (38.53%), Postives = 562/1059 (53.07%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQRPQV----------VDGVPLP----------------MDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPEELDKE-WDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRR--------GRDGPQVSAAQSAFDAMQGQKADDGLARGEGSRFPYLKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRRVREVERRHGGTVQELSARIVPADVQ-------------PLGRPKLPHPVPRLSPVASPFLPAPP--------------GPSPEEKNMKNKSVAQQLREKL--------------KKPKKELENGVAGVEGGPEASDAPIGDDCEA--GMKHELD-----HTTKMETPVIEASDS-------AKPVKRVKTEDSNIMDDKADLVTLKEPGSVT-ALGMSVEMCLETTASAQDSQEGTKELEEDDEDVGDDAMGEEEGKEEFEDPHIVVPPELEGTELAAAQKELAERVKSKQEARLDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTPHASALLPGLTHPPRRLTDRDLQPRRPPRLGRGIN 968
            MGVPA FRWLS+KYP  V  + E  P            V G PLP                +D+  PNPN  EFD LY+DMNGI+HPC+HPE +P P+TEA+M V +  Y +R+   +RPR+L+ +AIDGVAPRAKMNQQR+RRFR++ +A+ + E K A  +E  AMG  V  E  DK+ WD N ITPGT FM  LA+ LR++VA ++N+   W +++V+ SD S PGEGEHKIMD+IR++R+  GYDPNTRH+++GLDADLIML+LATHEP+F +LRE VF   R        G+ G   S    A    QG+         E ++    KPF  L +  LREYLE E  ++ G+PF ++ ER +DD+VFL FFVGNDFLPHLPSL+IR+GAID LL +++  LP MGDYLT+  G VN++    IL  + R EDE+F+RRR  EE    R +R  +     +G +    S + +P  +Q             P      P  V   +    PFLPA P                  E     N+S A+ L+ +L              ++ K EL+          EA  AP+ DD E   G+           +T  +  V E++DS       A+ VKR  +E++             EP   T A+  +V    +T A+   + +   E+ ED   V DD   +EEG     D  ++  P      +A A   +   V             E +DT++  E G+K+RYYQ K     LE    R+ + + Y+EGL WV  YYY+GC +W WFYP+H+APFASD  ++E+  + FE G P RP EQLM V P  S   +P P   LMTS+DSPI DFYP +  +D NGK M W  V LLPFID +RLL ++     +LSE E++RN +G  ++ +++ H  + A+  +   + +     V  ++ S+ G +G L  P PD     T    +P      L  + ++R + A +  P Q  PH S LLPG+    R LT RD    R     RG N
Sbjct:    1 MGVPALFRWLSKKYPHIVTSVKEDLPTKAQPGASRLDPVTGQPLPGSEDDPDGGGEDVEIPVDMSLPNPNGEEFDCLYLDMNGIVHPCTHPEGKPPPETEADMMVEIFAYTERVVNMIRPRKLLLIAIDGVAPRAKMNQQRSRRFRASQEAKTKAEEKEAAIREWEAMGKEVSDEYKDKKAWDQNAITPGTPFMDLLAASLRYWVASKLNSDPGWSKLQVVISDASVPGEGEHKIMDYIRRQRSHAGYDPNTRHVIYGLDADLIMLSLATHEPYFKVLREDVFAQDRKPRGCHRCGQPGHHSSNCTGAPKEKQGE-------FDEKNKITERKPFIFLDVSVLREYLEIEL-NVPGVPFPFDLERAIDDWVFLIFFVGNDFLPHLPSLEIREGAIDTLLAIWRTSLPQMGDYLTS-HGKVNMKSAQFILDGLARREDEIFQRRRDAEE----RQDRNEKRRRLGNGASDDSPSRKPLPGSMQMPNGTEYVEVQSLPSSGRSTPSMVGHKNGPLPPFLPARPTTIVPAVSQAQLMASSIAESSRAANQSAAKSLKAQLGVTAAANSANLSAAERLKAELKARKTTATQSSEA--APVSDDDEQLYGIASSSGDGVPPSSTDAQETVSESADSSQELYNAARGVKRKASEEAEADRSSGPAPVSDEPTPETHAIAAAVS---DTVAAGLQAGQDESEVAEDAAIVLDD---DEEGPA---DTSVLSGP------VAPAPLRITGNVA------------EQDDTVKLWEPGYKERYYQQKFGVP-LEDKAFRDQIVKNYMEGLSWVLAYYYQGCPSWQWFYPWHFAPFASDFTDLEKLDIQFELGAPFRPFEQLMGVFPAASRKHIPAPFHDLMTSEDSPIIDFYPEEFMIDMNGKKMLWQGVALLPFIDSSRLLAALDLHYHELSEDEERRNGLGSDVVFVNDNHGLYEALTNLYGKKPST--IPVAFDSKSSDGLAG-LAAPDPDCVPGTT--FFSPFDDSFGLPHINNDRSVSAVYHFPPQLKPHRSMLLPGVQIAKRVLTARDKDSVR-----RGTN 1006          

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|1034737648|gb|OAV91706.1| (hypothetical protein PTTG_06483 [Puccinia triticina 1-1 BBBD Race 1])

HSP 1 Score: 633.254 bits (1632), Expect = 0.000e+0
Identity = 383/1021 (37.51%), Postives = 553/1021 (54.16%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQRP--QVVDGVP--LPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMG--LPVPPEELDKEWDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDAMQGQ-KADDGLARGEGSRFPY-LKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRRVREVERRHGGTVQELSARIVPADVQPLGRPKLPHPVPRLSPVASPFLPAPPGPSPEEKNMKNK-----SVAQQLREKLKKPKKELENGVAGVEGGPEASDAPIGDDCEAGMKHELDHTTKMETPVIEASDSAKPVKR--VKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLETTASAQDSQEGTKE------LEEDDEDVGDDAMGEEEGKEEFEDPHIVVPPELEGTELAAAQKELAERVKSKQEAR---------------LDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTPHASALLPGLT--HPPRRLTDRDLQPRRPPRLGRGINIAELGTQ-FGFNEVH 982
            MGVPA FRWLS+KYP+ V  ++E  P    V+G    +P+D+   NPN +EFDNLY+DMNGI+HPC+HPE +  P+TEAEM + V  Y +R+   VRPR+L+ +AIDGVAPRAKMNQQR+RRFR+A +A+ + E K A  +E +  G  LP   +   K WDSN ITPGT FM  L   LR+++ +++NT K W Q++V+ SD S PGEGEHKIMDF+R++R QP YDPNT+H+++GLDADLIML+LATHEPHF +LRE VF  ++   G   +  Q    + Q Q K  + +   +  + P   KPF  L +  LREYL  E  ++ GLPF ++ ER +DD+V L FFVGNDFLPHLPSL+IR+GAID LL ++K  L  MG +LT   G +NL++ +IIL  +   EDE+FRRRR  EE+      RR  E + R        +++  P   +  G P              P L  PP P+    N+ +        +  +       K       A +             D  A +K EL  T ++ TP     ++  P++   V    SN++    D   + +P   T+ G   +   + +A A ++ +   E        + D  +  +  G++   +E E       PE+  TE +A   E  +  ++  +                 L     E +DT++  E G+++RY++ K   + L     +  + + Y+EGL WV  YYY+GCQ+W WFYP+HY+PFASD  N++ + + F+ G+P +P EQLM V P  S   +P     LM + DSPIKDFYP D E+D NGK M W  + LLPFI++ RLL ++   E+ L   +K RN  G  ++   E HP +    ++ +     ++  V ++ A A G  G+ L P PD    +        +P   L D+ +++ + A F +PSQ  PH S LL G+T   P     DRDL         RG N A  G Q +G +  H
Sbjct:    1 MGVPALFRWLSKKYPKVVEQVIEDVPAQNAVEGGTEEVPIDMSQRNPNGIEFDNLYLDMNGIVHPCTHPEGKKPPETEAEMMIEVFKYTERVVNMVRPRKLLMIAIDGVAPRAKMNQQRSRRFRAAQEAKVKEEEKQALIEEYIQQGKELPEDYKTNKKAWDSNAITPGTPFMTLLTESLRYWIVYKMNTDKGWSQIQVILSDASVPGEGEHKIMDFVRRQRTQPSYDPNTKHVIYGLDADLIMLSLATHEPHFKVLREDVFADQKKGKG-CYTCGQLGHHSSQCQGKPKEKVDEHDTQQKPVERKPFIFLDVGILREYLAIEL-NIGGLPFPFDLERAIDDWVLLIFFVGNDFLPHLPSLEIREGAIDTLLKIWKTNLGRMGGFLTD-CGALNLKRTEIILEGLAAREDEIFRRRREAEERQDQSAKRRKIESDNREAHIKSSSTSQHQPTSNE--GNP--------------PTLSLPPKPNFSLGNVGSTIPGLGGTSLSMMSNADVVKNRFAIRTANM-------------DAAAMLKAELSGTLELPTPAASGDNAVTPMETLDVTKPTSNVLLQAQDSSDVIQPVPTTSQGKPSDSVNDGSADATNANDSCHEPVTGPPNADTDPTIKPNTPGQKRKADELES----ADPEIGATEDSAVLSEDDDEDEAPGDVSVFVPGEPSRPAPLKMLGNNMVEQDDTVKLWEPGYRERYFRQKFGIE-LNDEVEKRKIVKHYVEGLAWVLAYYYQGCQSWQWFYPYHYSPFASDFTNLQEFEIKFDLGQPFKPYEQLMGVFPAASRCHIPEVYHELMMNDDSPIKDFYPEDFEIDMNGKKMLWQGIALLPFIEQDRLLNNMKDCENNLKAEDKIRNEPGHVVMFCGEAHPQYENFCKIYQKR--DNKEAVTLDPAHANGVFGSAL-PDPDC---IPGSTFYSPLPTVGLPDISNDKSIFARFFVPSQLVPHRSLLLSGVTLERPTLNNHDRDL-------TRRGGNQAHGGNQHYGNDGFH 971          

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|909612499|gb|KNF02723.1| (hypothetical protein PSTG_04009 [Puccinia striiformis f. sp. tritici PST-78])

HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0
Identity = 398/1016 (39.17%), Postives = 560/1016 (55.12%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQRP---QVVDGVP-LPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPE-ELDKE-WDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDAMQGQ-KADDGLARGEGSRFPY-LKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEE-QHQARLNRRV----REVERRHGGTVQ---ELSARIVPADVQPLGRPKLPHP---------VPRLSPVASPFLPAPPGPSPEEKN-----MKNKSVAQQLREKLKKPKKELENGVAGVEGGPEASDAPIGDDCEAGMKHELDHTTKMETPVIEASDSAKPVKRVKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLETTASAQDSQEGTKELEEDDEDVGDDAMGEEEGKEEFEDPHIVVPP--------ELEGTELAAAQKELAERVKSKQEARLDRLKE---EIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTPHASALLPGLTHPPRRLTDRDLQPRRPPRLGRGINIAELGTQ 975
            MGVPA FRWLS+KYP+ V  ++E  P   QV  G   +P+D+   NPN MEFDNLY+DMNGI+HPC+HPE +  P+TEAEM + V  Y +R+   VRPR+L+ +AIDGVAPRAKMNQQR+RRFR++ +A+ + E K A  +E +  G  +P E + DK+ WDSN ITPGT FM  L   LR+++ +++NT K W Q++V+ SD S PGEGEHKIMDF+R++R QP YDPNT+H+++GLDADLIML+LATHEPHF +LRE VF  ++   G   +  Q    + Q Q K  + +   +    P   KPF  L +  LREYL  E  +L GLPF ++ ER +DD+V L FFVGNDFLPHLPSL+IR+GAID LL ++K  L  MG YLT   G +NL++ +IIL  +   ED++FRRRR  EE Q QA   R++    R+V  +   T +    +S +  P+ +    +P +P           +P L   +   +      S   KN       N   A  LR +L     EL    A V+  P+ S     D  +A    + + TT++E+     ++SA P++     D N    +    +  +P  V  L  S +   E  AS QD   G  E E D        + ++   +E E    V+ P        E E  E A     +    +  + A L  L     E +DT++  E G++ RY++ K   + L+    +  + + Y+EGL WV  YYY+GCQ+W WFYP+HY+PFASD  N+E   + F  G+P +P EQLM V P  S   +P     LM   DSPIKDFYP D E+D NGK M W  + LLPFI++ RLL S+ A E  L   +K RN  G  ++   E HP +    ++ +     ++  V ++ A A G  G+ L P PD    +        +P   L D+ +++ + A F + SQ  PH S LL G+      L + D +  R     RG + A+ G Q
Sbjct:    1 MGVPALFRWLSKKYPKVVEQVIEDVPGQNQVEGGTEEIPIDMSQRNPNGMEFDNLYLDMNGIVHPCTHPEGKKPPETEAEMMIEVFKYTERVVNMVRPRKLLMIAIDGVAPRAKMNQQRSRRFRASQEAKVKEEEKQALIEEFIQQGKELPEEYKTDKKAWDSNAITPGTPFMTLLTESLRYWIIYKMNTDKGWSQIQVILSDASVPGEGEHKIMDFVRRQRTQPSYDPNTKHVIYGLDADLIMLSLATHEPHFKVLREDVFSDQKKGKG-CYTCGQPGHHSSQCQGKPKEKVDEHDTQSKPIERKPFIFLDVKILREYLAIEL-NLGGLPFPFDLERAIDDWVLLIFFVGNDFLPHLPSLEIREGAIDTLLKIWKTNLARMGGYLTD-CGELNLKRTEIILEGLASREDDIFRRRREAEERQDQASKRRKIDSDNRQVHIKSSSTNRPQPTVSNQGTPSTLNLPSKPAIPSGNLGQTAVSGIPGLGTTSLSMMSN----SDVIKNRFAIRTANMDAAAMLRAELSG-TLELPTSSASVDDTPKPS-TETSDVTQAESPTQAESTTQVESTT--QAESAAPLEAQNPSDPNPTTLE---TSPSKPSEVGDLEESTDKIQENAAS-QDPPVGLVEGEADSTIKPATPVHQKRKADELESTDTVIGPIEDSVVLSEDEDEEEAPGDVSVFVPGEPSRPAPLKMLGNNMVEQDDTVKLWEPGYRGRYFRQKFGIE-LDDEIEKRKIVKYYVEGLAWVLAYYYQGCQSWQWFYPYHYSPFASDFTNLEELEIKFNLGQPFKPYEQLMGVFPTASRSHIPEVYHELMLKDDSPIKDFYPEDFEIDMNGKKMLWQGIALLPFIEQDRLLTSMKACEDNLKAEDKIRNEPGHVVMFCGEAHPQYENFCKIYQKR--DNKEAVTLDPARANGVFGSAL-PDPDC---IPGSTFYSPLPTVGLPDISNDKSIFARFFVASQLVPHRSLLLSGVNLERSTLNNHDREVTR-----RGGSQAQGGNQ 989          

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|1149602133|gb|OON09324.1| (hypothetical protein BSLG_01604 [Batrachochytrium salamandrivorans])

HSP 1 Score: 628.246 bits (1619), Expect = 0.000e+0
Identity = 385/1008 (38.19%), Postives = 541/1008 (53.67%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQRPQVVDGVPLPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMG-LPVPPEELDKEWDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDAMQGQKADDGLAR-GEGSRFPYLKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRRVREVERR---HGGTVQELSARIVPADVQPLGRPKLPHPVPRLSPVASPFLPAPPGPSPEEKNMKNKSVAQQLREKLKKPKKELENGVAGVE-------GGPEASDAPIGDDCEAGMKHELDHTTKMETPVIEASDSAKPVKRVKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLE--------------TTASAQDSQEGTKELEEDDEDVGDDAMGEEEGKEEFEDPHIVVPPELEGTELAAA------------QKELAERVKSKQEARLDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDA--ALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTP----HASALLPGLTHPPRRLTDRDLQPRRPPRLG 964
            MG+PA FRWLS KYP+     VE+ P  +    +P+D   PNPN  E+D LY+DMNGIIHPC HPEDRP P TE EM++ +  Y+DRI + +RPR+++++AIDGVAPRAKMNQQR+RRFR+A + + ++E +   R+E+   G +   P    + +DSN ITPGT FM  LA  LR+YVA ++N    W  +KV+ SD S PGEGEHKIMD+IR++R QPGYDP T H+L+GLDADLIMLALATHEPHF ILRE VFF    ++G  V        A    K  + + + GE +     KP+  LH+  LREYLE E  ++  L F +N ER +DD+VFLCFFVGNDFLPHLPSL+IR+GAID L+ ++K+ L   G +LT   G ++L++V+ ++ E+G+VED  F++RR  +E  +    +R ++ + R   H G      A+ V  D       + P  + R++ + S   P         +N+         R  ++KP    +  VA  E          +++  P      +     LD    +++    AS   +P +   T    + D K D + +     V    +S  M +E               TAS+ D+    +  E  D    ++ + +     E E+        +E   +AA+             KE A  V S  EA         ED +R  E+GWK RYY+ K + + L+    RE +   Y+EGLCWV +YYY+G Q+W WFYP+HYAPFASD   I R  ++FE G P  P+EQLM VLP  S   +P  L  LMT   S I DFYP D  +D NGK   W  V LLPFIDE RLL++I  +  Q +E E +RN +G  ++ +   H  F      L   G  +   V ++      F G + P         R ++ +V   MP     D+E+N  L A + +P   TP    + +ALLPG+  P R L + D   R   R+G
Sbjct:    1 MGIPALFRWLSTKYPKVTSQCVEETPVTIGDQTIPVDASQPNPNGAEYDCLYLDMNGIIHPCCHPEDRPAPTTEDEMYIEIFKYIDRIMSIIRPRKVLYMAIDGVAPRAKMNQQRSRRFRAAQEEQLKNEAEDRIRKELEEKGHMAQDPVTKKQHFDSNCITPGTPFMDQLAICLRYYVAKKLNEDAGWDGLKVIISDASIPGEGEHKIMDYIRRQRNQPGYDPQTHHVLYGLDADLIMLALATHEPHFNILREDVFFKDGKQNGCFVCGQVGHMAAQCTGKPKEVIGKFGEQTTATVEKPYIFLHVGILREYLEIEL-AVPDLSFEWNLERAIDDWVFLCFFVGNDFLPHLPSLEIREGAIDQLIKLWKQYLSTWGGFLT-DSGDLDLKRVEEMIIELGKVEDVTFQKRRDEDEHRREMRKKRKQDAKNRLEGHHGQKAAAHAKRVHLD-------EAPEIMTRMADIES--FPV--------RNVDAHQRGDANRMAIRKPSGTKQANVAAAEMLRNRLSAAKKSTTLPASTPSAS-----LDQEATVKSIDAAASSHKRPAEENLTLSPTVTDVK-DTMVIPASHDVVKAPLSKRMAIEESDHPINDAHILKDETASSVDTP--MQSTETPDAAKIENVVADNVEVMEEEEEEEEEEVVIESMSVAASVPVHIPVPVLDTAKEAAPEVDSDDEAP--------EDNVRLWESGWKQRYYRKKFQVE-LDDSTFREAVVTAYVEGLCWVLKYYYQGVQSWKWFYPYHYAPFASDCGFISRLTISFEIGTPFLPVEQLMGVLPAASQSHIPPSLRPLMTEPTSEILDFYPLDFPIDLNGKKYTWQGVALLPFIDETRLLKAIVPIYDQFTEDETRRNTLGSEIVVVGGSHALFDDFCE-LYGRGVNNTEMVPIDPVKGGRFFGFVRPEDVFTVPGSRYSSPLVELGMP-----DIENNNSLSAKYQMP--VTPDGYKYQAALLPGVQMPLRVLDESD---RYAVRMG 961          

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|403163709|ref|XP_003323763.2| (hypothetical protein PGTG_05665 [Puccinia graminis f. sp. tritici CRL 75-36-700-3] >gi|375164418|gb|EFP79344.2| hypothetical protein PGTG_05665 [Puccinia graminis f. sp. tritici CRL 75-36-700-3])

HSP 1 Score: 622.854 bits (1605), Expect = 0.000e+0
Identity = 393/1030 (38.16%), Postives = 555/1030 (53.88%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQRP--QVVDGVP--LPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPE-ELDKE-WDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDAMQGQKADDGLARGEGSRFPY-LKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEE-QHQARLNRRV----REVERRHGGTVQELSARIVPADVQPLGRPKLPHPVPRLSPVASPFLPAPPGPSPEEKNMKNKSVAQQLREKLKKPKKE----LENGVAGVEGGPEASDAPIGDDCEAGMKHELDHT---------TKMETPVIEASDSAKPV--KRVKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLETTASAQDSQEGTK-------------ELEEDDEDVG--DDAMGEEEGKEEFEDP---HIVVPPELEGTELAAAQKELAERVKSKQEARLDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTPHASALLPG--LTHPPRRLTDRDLQPRRPPRLGRGINIAELGTQ-FGFNEVH 982
            MGVPA FRWLS+KYP+ V  ++E  P    VDG    +P+D+   NPN MEFDNLY+DMNGI+HPC+HPE +  P+TEAEM + V  Y +R+   VRPR+L+ +AIDGVAPRAKMNQQR+RRFR+A +A+ + E K A  +E    G  +P + + DK+ WDSN ITPGT FM  L   LR+++ +++NT K W Q++V+ SD S PGEGEHKIMDF+R++R QP YDPNT+H+++GLDADLIML+LATHEPHF +LRE VF  ++   G           +    K  + +   +  + P   KPF  L +  LREYL  E  ++ GLPF ++ ER +DD+V L FFVGNDFLPHLPSL+IR+GAID LL ++K  L  MG +LT   G +NL++ +IIL  +   EDE+FRRRR  EE Q Q+   R++    ++V  +   T Q L    +      L  P  P  +P    V      A PG      +M + +   + R  ++    +    L+  ++G    P  + AP GDD         D              ETP +  ++S  P   +    E  + +D      +  E     A     + C ++ A   D +  +              ELE  D  +G  DD++   E ++E E P    + VP E       A  K L   +             E +DT++  E G+++RY++ K   + L     +  + + Y+EGL WV  YYY+GCQ+W WFYP+HY+PFASD  N+E + + F  G+P +P EQLM V P  S   +P     LM + DSPIKDFYP + E+D NGK M W  V LLPFI++ RLL S+   E +L+  +K RN  G  ++   E HP +    ++ +     ++  V ++ A A G  G+ LP P      +        +P   L D+ +++ + A F +PSQ  PH S LL G  L  P     DRDL         RG N A  G Q +G +  H
Sbjct:    1 MGVPALFRWLSKKYPKVVEQVIEDVPAQNAVDGGTEEVPIDMSQRNPNGMEFDNLYLDMNGIVHPCTHPEGKKPPETEAEMMIEVFKYTERVVNMVRPRKLLMIAIDGVAPRAKMNQQRSRRFRAAQEAKVKEEEKQALIEEYKQQGKELPEDYKTDKKAWDSNAITPGTPFMTLLTESLRYWIVYKMNTDKGWSQIQVILSDASVPGEGEHKIMDFVRRQRTQPSYDPNTKHVIYGLDADLIMLSLATHEPHFKVLREDVFADQKKGKGCYTCGQLGHHSSQCQGKPKEKVDEHDTKQKPVERKPFIFLDVGILREYLAIEL-NIGGLPFPFDIERAIDDWVLLIFFVGNDFLPHLPSLEIREGAIDTLLKIWKTNLGRMGGFLTD-CGALNLKRTEIILEGLASREDEIFRRRREAEERQDQSAKRRKIDDDNKQVHIKSSTTNQPLPTSNLGNLSTTLNVPLKPTFLPE--DVRQTTQSAIPGLGNASLSMMSNADVVKNRFAIRTANMDAAAMLKAELSGTLELP--TSAPAGDDATKMTMESTDVALAESQVPLEANTETPGVTQAESDAPTGTQNPSNESHSAIDTSQGKPSNAENDESAATTNGKDSCQDSAAGPPDGETESTVKPPTPVQKRKADELESADPAIGPIDDSVVLSEDEDEDEAPGDVSVFVPGE---PSRPAPLKMLGNNMV------------EQDDTVKLWEPGYRERYFRQKFGIE-LNDEVEKRKIVKHYVEGLAWVLAYYYQGCQSWQWFYPYHYSPFASDFTNLEEFEIKFNLGQPFKPYEQLMGVFPAASRCHIPDVYHDLMMNDDSPIKDFYPEEFEIDMNGKKMLWQGVALLPFIEQDRLLNSMKDCEDRLAPEDKIRNEPGHVVMFCGEAHPQYENFCKIYQKR--DNKEAVTLDPAHANGVFGSALPDPD----CIPGSTFYSPLPTVGLPDISNDKSIFARFFVPSQLVPHRSLLLSGVALERPILNNHDRDL-------TRRGGNQAHGGNQHYGSDGFH 995          

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|939399404|emb|CEH14476.1| (5-3 exoribonuclease 2 [Ceraceosorus bombacis])

HSP 1 Score: 622.083 bits (1603), Expect = 0.000e+0
Identity = 382/1023 (37.34%), Postives = 552/1023 (53.96%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQRPQVV----------------DGVPLPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPE--ELDKEWDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVF-------------FGRRGRDGPQVSAAQS------AFDAMQGQKADDGLARGEGSRFPYLKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRR-VREVERRHGGTVQELSARIVPADVQPLGRPKLPH-PVPRLSPVASPFLPAPPGPSPEEKNMKNKSVAQQLREKLKKPKKELENGVAGVEGGPEASDAPIGDDCEAGMKHELDHTTKMETPVIEASDSAKP------VKRVKTEDSNIMD--DKADLVTLKEPGSVTALG-----------MSVEMCLETTASAQDSQEGTKELEEDDEDVGDDAMGEEEGKEEFEDPHIVVPPELEGTELAAAQKELAERVKSKQEARLD-RLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQ-----DVEDNRVLVAGFTLPSQTTPHASALLPGLTHPPRRLTDRDLQPRR 959
            MGVPA FRWLS+KYP+ V ++VE+  +                   GVP+P+D+   NPN  EFD LY+DMNGI+HPC+HPE +P P+TE +M + V  Y +R+   VRPR+L+ +AIDGVAPRAKMNQQR+RRFR+A +ARE+ E      +E  + GL V  E  E  K WD N ITPGT FM  LA+ LR++VA ++N    W+ ++V+ SD S PGEGEHKIMD+IR++RAQP +DPNT+H+++GLDADLIML+LATHEP+F +LRE VF              GR G        A+            Q  K  DG  +         KPF  L +  LREYLE E   + G+PF ++ ER +DD+VFL FFVGNDFLPHLPSL+IR+GAID LL ++KR LP +GDYLT   G+V+L+ +  ++  +  +ED++FR+RR  EE+ + R  RR V + ER+      E  AR      + L R    + PVPR +    P +                  ++ +R K           +A +E G    DA + D+        L    +++  +  A   AK       VK V      + D  D  D     E  + TA+            M  ++ +E    +   +   +++EE D DV  D  G E+ ++       VV  +++  + A +Q      + S  E +++     E EDT++  E G+++RYYQ K     L+    R  + ++Y+EGL WV  YYY+GC +W W+YP+H++PFA+D  +++   + F  G P RP +QLM VLP ES  ++P     LM  +DS I DFYP+   +D NGK M W  V +LPFIDE RLL ++  +  +L+  E +RN  G + L + + H  + A+  +     ++D   V ++ A +Q  SG ++  P         Q V      S L+     D+  +R + A ++ P QTTPH S LL G+  PP+ LT  D +  R
Sbjct:    1 MGVPALFRWLSKKYPKIVTNVVEEEQRYAPSGEGRGDDGQTDGEEKGVPIPIDISGKNPNGEEFDCLYLDMNGIVHPCTHPEGKPAPETEEDMMLEVFKYTERVVNMVRPRKLLMMAIDGVAPRAKMNQQRSRRFRAAQEAREKEEEMAKALEEWKSRGLTVLDEGSEKKKSWDRNAITPGTPFMDLLAASLRYWVAKKMNEDAGWKNLQVIISDASVPGEGEHKIMDYIRRQRAQPAHDPNTKHVIYGLDADLIMLSLATHEPYFKVLREDVFAQAPSSGGKGCHKCGREGHIAANCIGAEQPPPPGVDLTHKQAPKTQDGELK---------KPFIFLDVVVLREYLEVELH-VPGMPFAFDLERAIDDWVFLIFFVGNDFLPHLPSLEIREGAIDELLRIWKRELPKLGDYLTK-HGSVSLKNIQCVMDGIAVLEDDIFRKRRIAEERGEERQKRRKVEDHERQREQFAAEKRARGEEEAGEALHRGGTTYVPVPRANEGNEPSV---------------HGASEGVRAK----------ALAAIESG---DDAKVIDNLRTIRMANLSAADRLKAELAGAKAKAKAEAANARVKGVAPRQPQLSDAADDGDASKAPENAAPTAVAHTSSDNFAVFKMDEDVSIEAGGESAPVRSLKRQIEEVDGDV--DGEGNEKPEKAVALADGVV--QVKNADDADSQDRTDPVIASNLERKVNPDGTVEYEDTVKLWEPGYRERYYQQKFG-VGLDDIKLRRAIVKSYVEGLAWVLAYYYQGCPSWTWYYPYHFSPFAADFADLDELDIHFNLGTPFRPYDQLMGVLPAESRASIPPIFHPLMLEEDSEIIDFYPKTFAIDMNGKKMEWQGVAILPFIDEKRLLTALNRLYPRLTPDEVRRNAFGNNSLFVSDEHSLYDALCSLYAKRKSKDP--VPLDPALSQKLSGLVMADP---------QCVPGSTFSSPLERVGEVDISSDRSISAIYSFPEQTTPHKSVLLKGVRPPPKVLTQDDREWTR 968          

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|1129840525|emb|SHO78613.1| (Similar to S.cerevisiae protein RAT1 (Nuclear 5' to 3' single-stranded RNA exonuclease) [Malassezia sympodialis ATCC 42132])

HSP 1 Score: 621.313 bits (1601), Expect = 0.000e+0
Identity = 380/972 (39.09%), Postives = 534/972 (54.94%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQRPQVV---DGVP--LPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPEELDKEWDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDA-MQGQKADDGLARGEGSRFPYLKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRRVREVER--RHGGTVQELSARIVPADV----QPLGRPKLPHPVPRLSPVASPFLPAPPG----PSPEEKNMKNKSVAQQLREKL--KKPKKELENGVAGVEGGPEASDAPIGDDCEAGMKHELDHTTKMETPVIEASDSAKPVKRVKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLETTASAQDSQEGTKELEEDDEDVGDDAMGEEEGKEEFEDPHIVVPPELEGTELAAAQKELAERVKSKQEARLDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTPHASALLPGLTHPPRRLTDRD 954
            MGVPA FRWLS+KYP+ V  + E+ P  +   DG P  +P+D+  PNPN  EFD LY+DMNGI+HPC+HPE +  P+TE EM   V  Y +R+   +RPR+L+ +AIDGVAPRAKMNQQR+RRFRSA DA+   + +    +E    G     E + K WDSN ITPGT FM  LAS LR+++A ++NT   W+ + V+ SD S PGEGEHKIMD+IR++R+ P +DPN +H+++GLDADLIML+LATHEPHF +LRE VF       G      +  F A  +G+   +  + G+       KPF  L +PTLREYL  E ++     F ++ ER +DD+VFL FFVGNDFLPHLPSL+IR+GAID LL ++KR LP M  Y+T   G V LE    IL  + + ED++FR+R+  EE+ +A   R      R  +HG T      +  PA      QP G  K   PVP       P + A           +  M N S A+++R +L  KK  K   +   GV    +A      D        +LD         + A+D+   V+R K+  S     K  LV         A+    ++  E TA +    E   ++EE +E +        E  +  ED    VPPE          K+L  +V +           + EDT++  E G+++RYY+ K   + L     R  + + Y+EGLCWV  YYY+GC +W W+YP+HYAPFA+D  ++    + F   KP RP EQLM VLP  S   LP P   LMT+++S I DFYP++  +D NGK M W  V LLPFIDE RLL ++     QL++ E +RN  G ++L + +    F  ++R L  + ++ RA   ++ +     +G+L     D+A    +   +P      ++D+ +++ + A +  P Q TPH S LL G+  P RRLT  D
Sbjct:    1 MGVPALFRWLSKKYPKIVQSVEEEEPGQMPGPDGAPVDIPVDISRPNPNGEEFDCLYLDMNGIVHPCTHPEGKSPPETEEEMMAEVFKYTERVVNMIRPRKLLMMAIDGVAPRAKMNQQRSRRFRSAQDAKILQDQREEELEERRKRGQASEDEVVKKSWDSNAITPGTPFMDLLASSLRYWIAQKLNTDPGWKNLCVVLSDASVPGEGEHKIMDYIRRKRSDPNHDPNMKHVIYGLDADLIMLSLATHEPHFKVLREDVFAQDSKNKGCHRCGQEGHFAANCKGEPPKEVKSSGK-------KPFIFLDVPTLREYLAVELQT-PSTSFPFDLERAIDDWVFLIFFVGNDFLPHLPSLEIREGAIDTLLRIWKRELPRMTGYVTN-NGRVELENAQFILDGLAQAEDDIFRKRKETEERQEANKRRHASSNYRDVQHGDTTYVEVKKAAPASKALPPQPQGTTK---PVPGARHGKGPVMMAEDSMDVVKHRAQIRMANISAAEEMRAELQGKKNAKRTSDEQDGVRKRTKADP----DASAESTVFKLDEE-------VPANDTVPRVERAKSLASATEGGKPVLVK-------PAVDAPADIKTEETADSASQIE--PKVEESNESL-------REEDDSIEDDDDDVPPET---------KQLERKVNADGTV-------DYEDTVKLWEPGYRERYYRQKFGVE-LSDTKFRREVVQAYVEGLCWVLAYYYQGCPSWKWYYPYHYAPFAADFTDVASLEIKFADSKPFRPFEQLMGVLPAASKSNLPEPFQWLMTNEESEILDFYPKEFSIDMNGKKMAWQGVALLPFIDETRLLTALKQRYDQLTDDEVRRNSFGRNVLFVGKDADLFPVVSR-LYTKRSEGRAE-PIDTSRISQMAGSLT---ADSACVPGSTYYSPLTSLPDMKDIPNDKSISAHYDFPEQITPHRSTLLRGVRLPSRRLTPAD 911          

BLAST of EWM27232.1 vs. NCBI_GenBank
Match: gi|913867647|gb|KNZ57980.1| (hypothetical protein VP01_2023g2 [Puccinia sorghi])

HSP 1 Score: 620.928 bits (1600), Expect = 0.000e+0
Identity = 399/1065 (37.46%), Postives = 565/1065 (53.05%), Query Frame = 0

Query:    1 MGVPAFFRWLSEKYPRTVLDMVEQRP--QVVDGVP--LPMDLRAPNPNNMEFDNLYIDMNGIIHPCSHPEDRPTPKTEAEMFVNVMVYVDRIFAAVRPRRLVFLAIDGVAPRAKMNQQRTRRFRSAGDARERHEIKLATRQEMVAMGLPVPPE-ELDKE-WDSNVITPGTEFMVTLASYLRFYVAHRVNTCKAWQQVKVLFSDGSEPGEGEHKIMDFIRKERAQPGYDPNTRHILHGLDADLIMLALATHEPHFTILREQVFFGRRGRDGPQVSAAQSAFDAMQGQ-KADDGLARGEGSRFPY-LKPFQMLHIPTLREYLEEEFRSLRGLPFGYNFERVVDDFVFLCFFVGNDFLPHLPSLDIRDGAIDFLLNVYKRVLPAMGDYLTAPGGTVNLEQVDIILAEVGRVEDEVFRRRRAGEEQHQARLNRRVREVERRHGGTVQELSARIVPADVQPL-----GRPKL----PHPVPRLSPVASPFLPAPPGPSPEEKNM---------------KNKSVAQQLREKLKKPKKELENGVAGVEGGPEAS-DAPIGDDCEAGMKHELDHTTKMETPVIEASDSAKPVKRVKTEDSNIMDDKADLVTLKEPGSVTALGMSVEMCLETTASAQDSQEGTKELEEDDEDVG--DDAMGEEEGKEEFEDP---HIVVPPELEGTELAAAQKELAERVKSKQEARLDRLKEEIEDTIRYHEAGWKDRYYQDKLKKQNLEKGGGREHMYRTYIEGLCWVFQYYYRGCQAWGWFYPFHYAPFASDLVNIERYHVAFEAGKPLRPLEQLMAVLPPESADALPVPLARLMTSQDSPIKDFYPRDVEVDPNGKAMPWLWVTLLPFIDEARLLESIAAVESQLSEAEKKRNVVGGSLLCLHEYHPTFRAIARVLEVEGAQDRAGVEVEAASAQGFSGTLLPPPPDAALRLTAQVVAPKMPPSVLQDVEDNRVLVAGFTLPSQTTPHASALLPGLTHPPRRLTDRDLQPRRPPRLGRGINIAELGTQ-FGFNEVHATAAHRLIVQNMPRGAGSGSMLIYQQNQQQRMSCGGYIGSGQTG 1026
            MGVPA FRWLS+KYP+ V  ++E  P    V+G    +P+D+   NPN +EFDNLY+DMNGI+HPC+HPE +  P+TEAEM + V  Y +R+   VRPR+L+ +AIDGVAPRAKMNQQR+RRFR+A +A+ + + K A  +E    G  +P + + DK+ WDSN ITPGT FM  L   LR+++ +++NT K W Q++V+ SD S PGEGEHKIMDF+R++R QP YDPNT+H+++GLDADLIML+LATHEPHF +LRE VF  ++   G   +  Q    + Q Q K  + +   +  + P   KPF  L +  LREYL  E  ++ GLPF ++ ER +DD+V L FFVGNDFLPHLPSL+IR+GAID LL ++K  L  MG +LT   GT+NL++++ IL  +   EDE+FRRRR  EE+      RR  + +       Q+   +  P+  QPL     G P      P P      +      A PG      +M                N   A  L+ +L   K EL    AGV+  P+ S +       E     E    +  + P  E + S KP     +E ++ ++          PG         E  L  T++A   +   +ELE     +G  +D++   E ++E E P    + VP                E  +      L     E EDT++  E G+++RY++ K   + L     +  + + Y+EGL WV  YYY+GCQ+W W+YP+HY+PFASD  N+E + + F  G+P +P EQLM V P  S   +P     LM   DSPIKDFYP + E+D NGK M W  V LLPFI++ RLL S+   E  L+  +K RN  G  ++   E HP +    ++ +     +   V ++ A A G  G+   P PD    +        +P   L D+ +++ + A F + +Q  PH S LL G+T     L++ D    R     RG N A  G Q +G +  H         Q    G G G ++ YQ N       GGY    + G
Sbjct:    1 MGVPALFRWLSKKYPKVVEQVIEDVPAQNAVEGGTEEVPIDMSQRNPNGIEFDNLYLDMNGIVHPCTHPEGKKPPETEAEMMIEVFKYTERVVNMVRPRKLLMIAIDGVAPRAKMNQQRSRRFRAAQEAKVKEQEKQALIEEYKQQGKELPEDYKTDKKAWDSNAITPGTPFMTLLTESLRYWIVYKMNTDKGWSQIQVILSDASVPGEGEHKIMDFVRRQRTQPSYDPNTKHVIYGLDADLIMLSLATHEPHFKVLREDVFADQKKGKG-CYTCGQPGHHSSQCQGKPKEKVDEHDTMQKPVERKPFIFLDVGILREYLAVEL-NIGGLPFQFDLERAIDDWVLLIFFVGNDFLPHLPSLEIREGAIDTLLKIWKTNLARMGGFLTD-CGTLNLKRIETILEGLASREDEIFRRRREAEERQDQSAKRRKIDNDS------QQAHTQSSPSTTQPLPSSHQGTPSNLNLPPKPAFPSGNIGQTTQSAIPGLGTTSFSMLSNADIVKNRFIIRTANMDAAAMLKAELSG-KLELPKSAAGVQDSPKTSLETSDATQVEPNTSLEAQGPSDAKPPTPEPAHS-KPPDAGNSESADTINGDDSSRDAARPGLPDE-----ESDLTITSAAPAQKRKAEELESVQPVIGTIEDSVVLSEDEDEEETPGDVSVFVP---------------GEPSRPGPLKMLGNNMVEQEDTVKLWEPGYRERYFRQKFGIE-LNDEVEKRKIVKYYVEGLAWVLAYYYQGCQSWQWYYPYHYSPFASDFTNLEEFEINFNLGQPFKPYEQLMGVFPAASKSHIPEAYHGLMIDDDSPIKDFYPEEFEIDMNGKKMLWQGVALLPFIEQDRLLSSMKGCEEGLAPHDKARNEPGHVVMFCGEAHPQYENFCKIYQKR--DNTEAVTLDPAHANGVFGS-ASPDPDC---IPGSTFCSPLPTVGLPDISNDKSIFARFFVAAQLVPHRSLLLNGVTLERPTLSNYDRDKTR-----RGGNQAHGGNQHYGGDGFHQ--------QRRDFGDGPG-IMNYQNNHSSSGPAGGYWNPSRAG 1013