EWM27149.1, cds3455 (CDS) Nannochloropsis gaditana

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|585109329|gb|EWM27149.1| (retrograde transporter [Nannochloropsis gaditana])

HSP 1 Score: 1976.44 bits (5119), Expect = 0.000e+0
Identity = 972/972 (100.00%), Postives = 972/972 (100.00%), Query Frame = 0

Query:    1 MERYRQNHHAGPGQEKLYRPELLSSSQKAADGAADLAECFREVPAIFFHRDFQLSEPALFDRVVIRATPDTQERLSQYLDVVETCLLKQISSRSQHFFEALITLQDVRDRLAQACGQVLRLRSGLHDIDQKTVRGMIRIPRLAQRKVNLAALLEKLRLLQDVQRSTTLVQSLLGAGDFLGALDVLEDGQGLSEHSCVRLPLQQPELDEALKAQLVPRVRGLVRLCRLSKALERYRGRLTASLKEVVKTVLTEYLGVAEYQRGEDKVGADGAVNTPAEAASPPLPVNGTSGLPTAAAAATSTEENAGLLGKGVRAMKAESFLGCLKLCFEQMLLPMTGVGLIHTFLEEQISEELSRILDRRPVPSSSLHSTHQSQGELSTGSPGETGGEGSFGHVNREPSLPPSSSPAVSNSSRTDAGSVPPSPTPSPSNKFKTFLEKRRLETGGGASKSGTSTVGSLGIGGKQQTQAPFKDEDQIKVLELQACKKLNDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASSSVQSLQEDRLLSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRHRFVATEVIIGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGSAACRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGTIREKFSPASFLSSPATTSNSPSPTSLTLCLPPTDLNGGSASHEPILTPPRTSDAPASASSA 972
            MERYRQNHHAGPGQEKLYRPELLSSSQKAADGAADLAECFREVPAIFFHRDFQLSEPALFDRVVIRATPDTQERLSQYLDVVETCLLKQISSRSQHFFEALITLQDVRDRLAQACGQVLRLRSGLHDIDQKTVRGMIRIPRLAQRKVNLAALLEKLRLLQDVQRSTTLVQSLLGAGDFLGALDVLEDGQGLSEHSCVRLPLQQPELDEALKAQLVPRVRGLVRLCRLSKALERYRGRLTASLKEVVKTVLTEYLGVAEYQRGEDKVGADGAVNTPAEAASPPLPVNGTSGLPTAAAAATSTEENAGLLGKGVRAMKAESFLGCLKLCFEQMLLPMTGVGLIHTFLEEQISEELSRILDRRPVPSSSLHSTHQSQGELSTGSPGETGGEGSFGHVNREPSLPPSSSPAVSNSSRTDAGSVPPSPTPSPSNKFKTFLEKRRLETGGGASKSGTSTVGSLGIGGKQQTQAPFKDEDQIKVLELQACKKLNDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASSSVQSLQEDRLLSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRHRFVATEVIIGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGSAACRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGTIREKFSPASFLSSPATTSNSPSPTSLTLCLPPTDLNGGSASHEPILTPPRTSDAPASASSA
Sbjct:    1 MERYRQNHHAGPGQEKLYRPELLSSSQKAADGAADLAECFREVPAIFFHRDFQLSEPALFDRVVIRATPDTQERLSQYLDVVETCLLKQISSRSQHFFEALITLQDVRDRLAQACGQVLRLRSGLHDIDQKTVRGMIRIPRLAQRKVNLAALLEKLRLLQDVQRSTTLVQSLLGAGDFLGALDVLEDGQGLSEHSCVRLPLQQPELDEALKAQLVPRVRGLVRLCRLSKALERYRGRLTASLKEVVKTVLTEYLGVAEYQRGEDKVGADGAVNTPAEAASPPLPVNGTSGLPTAAAAATSTEENAGLLGKGVRAMKAESFLGCLKLCFEQMLLPMTGVGLIHTFLEEQISEELSRILDRRPVPSSSLHSTHQSQGELSTGSPGETGGEGSFGHVNREPSLPPSSSPAVSNSSRTDAGSVPPSPTPSPSNKFKTFLEKRRLETGGGASKSGTSTVGSLGIGGKQQTQAPFKDEDQIKVLELQACKKLNDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASSSVQSLQEDRLLSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRHRFVATEVIIGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGSAACRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGTIREKFSPASFLSSPATTSNSPSPTSLTLCLPPTDLNGGSASHEPILTPPRTSDAPASASSA 972          

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|553190818|ref|XP_005855268.1| (retrograde transporter [Nannochloropsis gaditana CCMP526] >gi|422293793|gb|EKU21093.1| retrograde transporter [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 786.178 bits (2029), Expect = 0.000e+0
Identity = 385/389 (98.97%), Postives = 387/389 (99.49%), Query Frame = 0

Query:  192 SEHSCVRLPLQQPELDEALKAQLVPRVRGLVRLCRLSKALERYRGRLTASLKEVVKTVLTEYLGVAEYQRGEDKVGADGAVNTPAEAASPPLPVNGTSGLPTAAAAATSTEENAGLLGKGVRAMKAESFLGCLKLCFEQMLLPMTGVGLIHTFLEEQISEELSRILDRRPVPSSSLHSTHQSQGELSTGSPGETGGEGSFGHVNREPSLPPSSSPAVSNSSRTDAGSVPPSPTPSPSNKFKTFLEKRRLETGGGASKSGTSTVGSLGIGGKQQTQAPFKDEDQIKVLELQACKKLNDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQ 580
            SEHSCVRLPLQQPELDEALKAQLVPRVRGLVRLCRLSKALERYRGRLTASLKEVVKTVLTEYLGVAEYQRGEDKVGADGAVNTPAEAASPPLPVNGTSGLPTAAAAATSTEENAGLLGKGVRAMKAESFLGCLKLCFEQMLLPMTGVGLIHTFLEEQISEELSRILDRRPVPSSSLHSTHQSQGELSTGSPGETGGEGSFGHVNREPSLPPSSSPAVSNSSRTDAGSVPPSPTPSPSNKFKTFLEKRRLETGGGASKSGTSTVGSLGIGGKQQTQAPFKDEDQIKVLELQACKKLNDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAE ++ 
Sbjct:  100 SEHSCVRLPLQQPELDEALKAQLVPRVRGLVRLCRLSKALERYRGRLTASLKEVVKTVLTEYLGVAEYQRGEDKVGADGAVNTPAEAASPPLPVNGTSGLPTAAAAATSTEENAGLLGKGVRAMKAESFLGCLKLCFEQMLLPMTGVGLIHTFLEEQISEELSRILDRRPVPSSSLHSTHQSQGELSTGSPGETGGEGSFGHVNREPSLPPSSSPAVSNSSRTDAGSVPPSPTPSPSNKFKTFLEKRRLETGGGASKSGTSTVGSLGIGGKQQTQAPFKDEDQIKVLELQACKKLNDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAESYLN 488          

HSP 2 Score: 586.26 bits (1510), Expect = 0.000e+0
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0

Query:  686 SYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGSAACRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGTIREKFSPASFLSSPATTSNSPSPTSLTLCLPPTDLNGGSASHEPILTPPRTSDAPASASSA 972
            SYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGSAACRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGTIREKFSPASFLSSPATTSNSPSPTSLTLCLPPTDLNGGSASHEPILTPPRTSDAPASASSA
Sbjct:  485 SYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGSAACRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGTIREKFSPASFLSSPATTSNSPSPTSLTLCLPPTDLNGGSASHEPILTPPRTSDAPASASSA 771          

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|223999917|ref|XP_002289631.1| (predicted protein [Thalassiosira pseudonana CCMP1335] >gi|220974839|gb|EED93168.1| predicted protein [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 274.633 bits (701), Expect = 7.891e-73
Identity = 166/467 (35.55%), Postives = 253/467 (54.18%), Query Frame = 0

Query:  496 DLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASSSVQSLQEDRLLSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRHRFVATEVIIGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGSAA----------CRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKE-YLDHA---------------RPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGTIREKFSPASFLSSPATTSNSPSPTS 936
            DL  + +  LL  RREA+  +    ++ LWD++++F   +E+ SG   +GL+STLL QAK FVE  H+  +  L+AALD+ERWVQ  V A+RQA +  L +GRA   +R                P   SI ++              S S+          R   T+  +   R+KVV+SA+LL+ ++   +  AA+F  LA +V+  V  LLRLFNTR   LVLGAGAI + ARL+SI AKHLA+ +QC+G++L +LPHVRAAL A L  KQ + L ++D+++ +Y EH E++L+K V+I+G ++ HS A   +  +++D   S A          C F++ V+  T  MH+VL  +LPP  ++D+F+R+F  L H++   Y D A                PTT  G+ ++V EV  +  +L+ L GV       ++  + ++       S P   +  PSP S
Sbjct:  972 DLSEKSVSELLRLRREAHSLVSFDGMRQLWDTSLTFTLQLERFSGRKAYGLRSTLLAQAKSFVERKHEANMSTLVAALDSERWVQCTVSADRQAALTRLCSGRAAFTSRK---------------PAAGSIDNI-------------ASPSVGGG-------RDKMTDAEVEGVRFKVVWSALLLLEMVMDDVACAAHFQTLATNVVGKVSELLRLFNTRATHLVLGAGAIHSAARLKSINAKHLAIVTQCIGIVLAILPHVRAALMAQLPAKQHSLLHDIDKIKADYTEHSEKILSKLVSIIGGIVEHSLA-AKIPSSNFDTRASTASTVEDDAVDCCPFIDSVMTNTKKMHQVLNVMLPPELLRDVFSRIFAYLDHKVPSLYKDVALKPTDPINPSQSRFSMPTTEEGKRQMVKEVECMTKTLNGLSGVEPWNFTAMK-VLEQELDIEEEESLPVVDTFMPSPVS 1401          

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|397568867|gb|EJK46393.1| (hypothetical protein THAOC_34941 [Thalassiosira oceanica])

HSP 1 Score: 272.707 bits (696), Expect = 2.202e-72
Identity = 158/446 (35.43%), Postives = 242/446 (54.26%), Query Frame = 0

Query:  496 DLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVG--------------------AERQAEIDHLMAGRAVLAARASSSVQSLQEDRLLSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRHRFVATEVIIGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGS------------AACRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYL---------DHARPTTNAGRERLVDEVSHLVTSLSMLRGV 900
            DL  + +  LL  R+EA+  +    ++ LWD++++F   +E+ SG   +GL+STLL QAK FVE  H+  +  L+AALD+ERWVQ +V                     AERQA +  L +GRA  ++R                P  D+      +S  RE                        T+  +   +YKVV+S +LL+ ++   +  AA+F  LA +V+  V  LLRLFNTR+  LVLGAGAI + ARL+SI AKHLA+ +QC+ L+L +LPHVRAAL A L  KQ A L +LD+++ +Y EH E+VL+K V+I+G L+ HS A   +  TD+DA                 C+F++ V+  T  M++VL+ +LP   ++D+F+R+F  L H++             + A PTT+ G+ R++ E+ ++  +L+ L+GV
Sbjct:  853 DLSEKSVSELLRLRKEAHSLVSFDGMRHLWDTSLTFTYQLEKFSGRKAYGLRSTLLAQAKSFVERKHESNMSALVAALDSERWVQCNVSLRLVLLNPSELHDLIFAQVSAERQAALTRLCSGRAAFSSRKQ--------------PGLDAAMTPAPVSTGREK----------------------MTDAEVEGVKYKVVWSCLLLLEMVMDDVACAAHFQTLAANVVGKVAELLRLFNTRSTHLVLGAGAIHSAARLKSINAKHLAIVTQCIALVLAILPHVRAALMAQLPAKQHALLCDLDKIKADYKEHSEKVLSKLVSIIGGLVEHSLA-PRISGTDFDARAKQFSEPDDPTTPVECCKFIDNVMTNTKKMYQVLFVMLPSELLRDVFSRIFAYLDHKVPSLFKAAVHSTDGNFAMPTTDEGKLRMISEIEYMFRTLNSLKGV 1261          

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|1149603335|gb|OON10428.1| (hypothetical protein BSLG_00752 [Batrachochytrium salamandrivorans])

HSP 1 Score: 218.009 bits (554), Expect = 1.312e-54
Identity = 212/781 (27.14%), Postives = 346/781 (44.30%), Query Frame = 0

Query:  207 DEALKAQLVPRVRGLVRLCRLSKALERYRGRLTASLKEVVKTVLTEYLGVAEYQRGEDKVGADGAVNTPAEAASPPLPVNGTSGLPTAAAAATSTEENAGLLGKGVRAMKAESFLGCLKLCFEQMLLPMTGVGLIHTFLEEQISEELSRILDRRPVPSSSLHSTHQSQGELSTGSPGETGGEGSFGHVNREPSLPPSSSPAVSNSSRTDAGSVPPSPTPSPSNKFKTFLEKRRLETGGGASKSGTSTVGSLGIGGKQQTQAPFKDEDQIKVLELQACKKLNDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASSSVQSLQEDRLLSFPKEDSISDVQDLSHSRESG-------ELSISSSISNDGHSENRHRFVATEVI-----------IGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWD-------ALGSAACRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNA--QALAELEGTIREKFSPASFLSSPATTSNSPSPTSLTLCLPPTDLNGGSASHEPILTPP 960
            +E L ++L P V GL+R  RL  AL  +R  L   +K +VK          +Y     ++G+     +P    +P            A A+  S +E    L   +R+M  + F   L   +   L  +     IH  L   I++   R +D   + S+S+ S   +   L+     ET  +             PS++    +    + GS+   P  S     + F+  RR    GG + + +S    +G              D       Q C +  D +L    DL       L+  R E N  ++  +   L+ ST  F+ + E + G   FGLKS +LT AK F+ H H     ++   ++ E+W Q DV  + Q   + L        A  S+    + ++RL S   E    DV D   SR+S        E+ ++SSI    + +   +    E+I           +   +Y VV S +L +  L  Y+N A   P+L  DV+  ++ LL+LFN+R  Q++LGAGA+Q+ A LRSITA+H+ALASQ +G ++ ++P++R  ++  L +KQQ  L + DR+ +++ EHQ  +  K V+IM + +   A+  G     WD       +   A    +  +IK TTT+H+VL + LP   ++ IF  +F     RL E        ++AG+ RL+ +V + +  LS L GV+     L      I+ K   +  L   AT + + S + L +  P   L+ GS+  +  L PP
Sbjct:  526 EEKLVSKLTPLVLGLLRTDRLGSALRAHRDDLIKQVKTMVK----------QYYPVISEIGS----TSPDPKETP------------ADASLQSKKEGQSALSLQLRSMTFDKFYAMLIRVYIASLHIIQRTSTIHQVLLSIITDAQKRGID---IGSNSVQSMAVA---LTLKVSPETATQ-------------PSTARRFLDDD-DELGSLAELPEMS-----QDFM--RRSSKKGGMADASSSASDLVG--------------DASATTYGQMCNESLD-VLTVTSDLIQARCAKLIGVRSEQNAKLNATDFYRLFGSTWEFVTSSELLCGRMCFGLKSVILTHAKAFLLHFHDEKSKQIAVLIENEQWAQADVPVDFQHITEQLQL------APLSAMTDRMDKNRLGSLSDE---LDVNDDISSRKSDPDDGSGDEMDLASSILIKANIKEMKKQQKKELIESAPKTLRYLAVDGGKYYVVGSVLLFLKTLTEYMNCAEKLPVLTTDVLNRILELLKLFNSRVCQVILGAGAMQS-AGLRSITARHIALASQTIGTVIAIIPYIRNGMSRLLSSKQQILLTDFDRILRDFKEHQNELYAKLVSIMNDRLGPHASKLG--AITWDNPDPKEISAEEAVTVHMAVLIKETTTLHKVLSKYLPGESLRSIFGEIFRSYVKRLDEEFHKIDLFSSAGKNRLLMDVQYFIRQLSALDGVDGPDSHLEVCVNNIKIKEKRSVHLMRTATRAGAASNSELKIPTPSQPLHSGSSPLQSPLAPP 1226          

HSP 2 Score: 77.0258 bits (188), Expect = 1.410e-10
Identity = 49/158 (31.01%), Postives = 86/158 (54.43%), Query Frame = 0

Query:   43 VPAIFFHRDFQLSEPALFD------RVVIRATPDT-------QERLSQYLDVVETCLLKQISSRSQHFFEALITLQDVRDRLAQACGQVLRLRSGLHDIDQKTVRGMIRIPRLAQRKVNLAALLEKLRLLQDVQRSTTLVQSLLGAGDFLGALDVLED 187
            VP+IF  +DF LS P  F        +V   T D+       Q+ ++ +LD VE  LL +IS RS  FF AL  LQ++ +   +   ++  LR+ L  +   T +  + + RL  R+ N+  L   ++L+ D++ + +++Q LLG  D++G LD++++
Sbjct:  158 VPSIFLDKDFDLSNPHTFSAACDYMDIVGSNTTDSSSINRLLQDTITHHLDTVEVHLLNEISRRSSSFFSALSNLQNLHNETEECVCKIQDLRTKLASVSLMTAQNGLEVVRLKHRRGNVGFLYGAVKLIADIKHTQSMIQVLLGQADYVGVLDLIKN 315          

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|1023243926|gb|KZT39716.1| (Vps54-domain-containing protein [Sistotremastrum suecicum HHB10207 ss-3])

HSP 1 Score: 215.312 bits (547), Expect = 1.042e-53
Identity = 161/491 (32.79%), Postives = 245/491 (49.90%), Query Frame = 0

Query:  476 KVLELQACKKLNDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASSSVQSLQEDRLLSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRHRFVATEVIIGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGE-LIAHSAANTGLRETDWDALGSAACR---FVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRL-KEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGTIREKFSPA----------------SFLSSPATTSNSPSPTSLTLCLP-PT 944
            K LEL + K    + L    +L       +L  R E + ++DL     L+DST  F+ A E I      GL+ T++ QA+ F+   HK  + R    ++ E+W   +V +  Q   D L     V AA       SL  ++ +  P  + I+     S S++              H E+R              + VV + + ++ LL  YL V  N P+L  D M  VV  L+ FN+RT Q+VLGAGA++ +A L++ITAKHLALASQ L +++ L+P+VR     HL  KQ   LIE D+L+++Y EHQ  + +K V IM + L  H  +   L E DW    SAA +   ++E +IK TTT+H+VL++ LP    + + ++VF  + H+L +EY     P+ +A +ERL+ +V +L   LS L+GV   +   LE  + EK  P                 S  +S   T+N P+P + T  +P PT
Sbjct:  573 KDLELPSLKSDLSDTLGAAAELANTRASKVLAIRSEQHAALDLTSFVNLFDSTWQFVVACEVICQKMIVGLRGTIVNQARSFLSEFHKERLSRSAKLVEDEQWAVVEVSSVAQHSSDQL-----VDAAVRDPPEFSL--NKPVQTPATNGITKKNGASSSKQL-------------HIEDR-------------TFHVVAATLDVLGLLGDYLRVIINLPLLTTDAMSRVVEFLKAFNSRTCQVVLGAGAMR-SAGLKNITAKHLALASQSLSVMIALIPYVRETFRRHLNPKQAVMLIEFDKLKRDYQEHQNEIHSKLVGIMSDRLQVHCKS---LPEIDWSLRLSAAPKVNPYMEGLIKDTTTLHKVLFKFLPGPSAEFVMSQVFATINHQLSEEYGKIELPSIDA-KERLLSDVRYLRDKLSTLKGV-GNSTGMLEIVVTEKRIPGPVSPAPPAKVPSPPPISVTTSQEKTTNVPNPPARTNSVPTPT 1024          

HSP 2 Score: 89.7373 bits (221), Expect = 1.608e-14
Identity = 53/174 (30.46%), Postives = 88/174 (50.57%), Query Frame = 0

Query:   33 AADLAECFREVPAIFFHRDFQLSEPALFDRVVIRATPDTQ-------------ERLSQYLDVVETCLLKQISSRSQHFFEALITLQDVRDRLAQACGQVLRLRSGLHDIDQKTVRGMIRIPRLAQRKVNLAALLEKLRLLQDVQRSTTLVQSLLGAGDFLGALDVLEDGQGLSE 193
            AA  A     +P ++F   F L+ PA F  V   A  +               E++S Y D +E  L+++IS RS  FF AL  LQD++    +   ++  LR  L ++D+K  R  + + RL +RK NL ++ E ++++  V     + +  +GAGD+  AL V+E  +G+ E
Sbjct:  173 AAKQAPPLSTIPQVYFEESFDLTNPATFAAVTEVAGTNNPLDPGDPAYSGPLLEKMSHYTDTLELHLIREISLRSTSFFAALSNLQDLQTESERCLNRISHLREILKEVDEKGARRGLEVVRLEKRKENLHSVAEGVKVVSGVGEMMNVARGFVGAGDWTEALSVIEGIEGIWE 346          

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|1023190776|gb|KZS88368.1| (Vps54-domain-containing protein [Sistotremastrum niveocremeum HHB9708])

HSP 1 Score: 215.312 bits (547), Expect = 1.099e-53
Identity = 161/491 (32.79%), Postives = 245/491 (49.90%), Query Frame = 0

Query:  476 KVLELQACKKLNDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASSSVQSLQEDRLLSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRHRFVATEVIIGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGE-LIAHSAANTGLRETDWDALGSAACR---FVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRL-KEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGTIREKFSPA----------------SFLSSPATTSNSPSPTSLTLCLP-PT 944
            K LEL + K    + L    +L       +L  R E + ++DL     L+DST  F+ A E I      GL+ T++ QA+ F+   HK  + R    ++ E+W   +V +  Q   D L     V AA       SL  ++ +  P  + I+     S S++              H E+R              + VV + + ++ LL  YL V  N P+L  D M  VV  L+ FN+RT Q+VLGAGA++ +A L++ITAKHLALASQ L +++ L+P+VR     HL  KQ   LIE D+L+++Y EHQ  + +K V IM + L  H  +   L E DW    SAA +   ++E +IK TTT+H+VL++ LP    + + ++VF  + H+L +EY     P+ +A +ERL+ +V +L   LS L+GV   +   LE  + EK  P                 S  +S   T+N P+P + T  +P PT
Sbjct:  573 KDLELPSLKSDLSDTLGAAAELANTRASKVLAIRSEQHAALDLTSFVNLFDSTWQFVVACEVICQKMIVGLRGTIVNQARSFLSEFHKERLSRSAKLVEDEQWAVVEVSSVAQHSSDQL-----VDAAVRDPPEFSL--NKPVQTPATNGITKKNGASSSKQL-------------HIEDR-------------TFHVVAATLDVLGLLGDYLRVIINLPLLTTDAMSRVVEFLKAFNSRTCQVVLGAGAMR-SAGLKNITAKHLALASQSLSVMIALIPYVRETFRRHLNPKQAVMLIEFDKLKRDYQEHQNEIHSKLVGIMSDRLQVHCKS---LPEIDWSLRLSAAPKVNPYMEGLIKDTTTLHKVLFKFLPGPSAEFVMSQVFATINHQLSEEYGKIELPSIDA-KERLLSDVRYLRDKLSTLKGV-GNSTGMLEIVVTEKRIPGPVSPAPPAKVPSPPPISVTTSQEKTTNVPNPPARTNSVPTPT 1024          

HSP 2 Score: 89.7373 bits (221), Expect = 1.664e-14
Identity = 53/174 (30.46%), Postives = 88/174 (50.57%), Query Frame = 0

Query:   33 AADLAECFREVPAIFFHRDFQLSEPALFDRVVIRATPDTQ-------------ERLSQYLDVVETCLLKQISSRSQHFFEALITLQDVRDRLAQACGQVLRLRSGLHDIDQKTVRGMIRIPRLAQRKVNLAALLEKLRLLQDVQRSTTLVQSLLGAGDFLGALDVLEDGQGLSE 193
            AA  A     +P ++F   F L+ PA F  V   A  +               E++S Y D +E  L+++IS RS  FF AL  LQD++    +   ++  LR  L ++D+K  R  + + RL +RK NL ++ E ++++  V     + +  +GAGD+  AL V+E  +G+ E
Sbjct:  173 AAKQAPPLSTIPQVYFEESFDLTNPATFAAVTEVAGTNNPLDPGDPAYSGPLLEKMSHYTDTLELHLIREISLRSTSFFAALSNLQDLQTESERCLNRISHLREILKEVDEKGARRGLEVVRLEKRKENLHSVAEGVKVVSGVGEMMNVARGFVGAGDWTEALSVIEGIEGIWE 346          

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|575483838|ref|XP_006681213.1| (hypothetical protein BATDEDRAFT_90790 [Batrachochytrium dendrobatidis JAM81] >gi|328768087|gb|EGF78134.1| hypothetical protein BATDEDRAFT_90790 [Batrachochytrium dendrobatidis JAM81])

HSP 1 Score: 208.764 bits (530), Expect = 1.225e-51
Identity = 142/496 (28.63%), Postives = 251/496 (50.60%), Query Frame = 0

Query:  489 EILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASS--SVQSLQED-----RLLSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRH------RFVATEVIIGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGSAACRFVEEV-------IKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGT-----IREKFSPASFLSSPATTSNSPSPTSLTLCLPPTDLNGGSASHEPILTP 959
            ++L T+ DL +     L+  R E N  ++ A+   ++ +T  F+ + E I G   FGLKS +LT AK F+ H H     ++   ++ E+WV  D+  + Q   + L     ++   + +  S  S+ ED      +LS   ED  S  +++  +  +   + ++ I ++  ++ +       R++A    +  E+Y VV S +L +  L  Y+N A + P+L  DV+  ++ LL+LFN+R  Q++LGAGA+Q+ A L+SITA+H+ALA+Q +G+I+ ++P++R  ++  L  KQQ  L + DR+ +++ EHQ  +  K ++IM + +A  A+  G     WD   S      E V       IK TTT+H+VL + LP   +++I   +F     RL          +++G+ RL+ +V + +  LS L G++    + LE       I+EK        S A  ++      L     P+  NG +    P+ +P
Sbjct:  749 DVLSTISDLIHARCAKLIGVRSEQNAQLNAADFYRIFGATWEFITSSEIICGRICFGLKSVILTHAKAFISHFHDEKSKQIAVLVENEQWVHADIPIDFQHITEALQLDPKLIGDESENKESYSSISEDFDVEEDVLSRHSEDDNSTTEEMDLTSFAFMKANAAEIKSEKIAKQQENAPKTLRYLA----VDGEKYYVVGSVLLFLKTLTDYINCAESLPVLTTDVLNRILELLKLFNSRVCQVILGAGAMQS-AGLKSITARHIALAAQSIGVIIAIIPYIRKGISRLLSPKQQVLLSDFDRILRDFREHQNELYAKLISIMNDRLALHASKLG--AISWDNPDSKEISAEETVTVHMAVLIKETTTLHKVLSKYLPADALKNIMGEIFRNYVQRLDMEFRKIDLFSSSGKNRLLMDVQYFIGQLSALDGIDGPG-SHLEVCVNNIKIKEK-------RSAAVLNSGRGGAGLNTLAIPSPFNGSTGGTSPLKSP 1229          

HSP 2 Score: 84.3445 bits (207), Expect = 9.291e-13
Identity = 53/157 (33.76%), Postives = 83/157 (52.87%), Query Frame = 0

Query:   43 VPAIFFHRDFQLSEPALFD------RVVIRATPDT-------QERLSQYLDVVETCLLKQISSRSQHFFEALITLQDVRDRLAQACGQVLRLRSGLHDIDQKTVRGMIRIPRLAQRKVNLAALLEKLRLLQDVQRSTTLVQSLLGAGDFLGALDVLE 186
            +P IF  +DF L+ P  F+       VV     D        Q++L+ +LD VE  LLK+IS RS  FF AL  LQ + +   +  G++  LR  L  +  +T    + + RL  R+ N+  L   ++LL D++ S   +Q+LLG  D++G LD++E
Sbjct:  169 IPTIFMDKDFDLTNPHTFNAASEYMNVVGSNASDENVANRQLQDKLTHHLDTVEVHLLKEISRRSSSFFSALSNLQSLHNETEECVGKIQDLRKKLASVSARTAENGLEVVRLKHRRGNVGMLYGAVKLLVDIKHSQPTIQALLGQADYVGVLDLIE 325          

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|1028576078|gb|OAJ44382.1| (hypothetical protein BDEG_27616 [Batrachochytrium dendrobatidis JEL423])

HSP 1 Score: 208.764 bits (530), Expect = 1.449e-51
Identity = 142/496 (28.63%), Postives = 251/496 (50.60%), Query Frame = 0

Query:  489 EILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASS--SVQSLQED-----RLLSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRH------RFVATEVIIGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIAHSAANTGLRETDWDALGSAACRFVEEV-------IKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHLVTSLSMLRGVNAQALAELEGT-----IREKFSPASFLSSPATTSNSPSPTSLTLCLPPTDLNGGSASHEPILTP 959
            ++L T+ DL +     L+  R E N  ++ A+   ++ +T  F+ + E I G   FGLKS +LT AK F+ H H     ++   ++ E+WV  D+  + Q   + L     ++   + +  S  S+ ED      +LS   ED  S  +++  +  +   + ++ I ++  ++ +       R++A    +  E+Y VV S +L +  L  Y+N A + P+L  DV+  ++ LL+LFN+R  Q++LGAGA+Q+ A L+SITA+H+ALA+Q +G+I+ ++P++R  ++  L  KQQ  L + DR+ +++ EHQ  +  K ++IM + +A  A+  G     WD   S      E V       IK TTT+H+VL + LP   +++I   +F     RL          +++G+ RL+ +V + +  LS L G++    + LE       I+EK        S A  ++      L     P+  NG +    P+ +P
Sbjct:  775 DVLSTISDLIHARCAKLIGVRSEQNAQLNAADFYRIFGATWEFITSSEIICGRICFGLKSVILTHAKAFISHFHDEKSKQIAVLVENEQWVHADIPIDFQHITEALQLDPKLIGDESENKESYSSISEDFDVEEDVLSRHSEDDNSTTEEMDLTSFAFMKANAAEIKSEKIAKQQENAPKTLRYLA----VDGEKYYVVGSVLLFLKTLTDYINCAESLPVLTTDVLNRILELLKLFNSRVCQVILGAGAMQS-AGLKSITARHIALAAQSIGVIIAIIPYIRKGISRLLSPKQQVLLSDFDRILRDFREHQNELYAKLISIMNDRLALHASKLG--AISWDNPDSKEISAEETVTVHMAVLIKETTTLHKVLSKYLPADALKNIMGEIFRNYVQRLDMEFRKIDLFSSSGKNRLLMDVQYFIGQLSALDGIDGPG-SHLEVCVNNIKIKEK-------RSAAVLNSGRGGAGLNTLAIPSPFNGSTGGTSPLKSP 1255          

HSP 2 Score: 84.3445 bits (207), Expect = 8.897e-13
Identity = 53/157 (33.76%), Postives = 83/157 (52.87%), Query Frame = 0

Query:   43 VPAIFFHRDFQLSEPALFD------RVVIRATPDT-------QERLSQYLDVVETCLLKQISSRSQHFFEALITLQDVRDRLAQACGQVLRLRSGLHDIDQKTVRGMIRIPRLAQRKVNLAALLEKLRLLQDVQRSTTLVQSLLGAGDFLGALDVLE 186
            +P IF  +DF L+ P  F+       VV     D        Q++L+ +LD VE  LLK+IS RS  FF AL  LQ + +   +  G++  LR  L  +  +T    + + RL  R+ N+  L   ++LL D++ S   +Q+LLG  D++G LD++E
Sbjct:  195 IPTIFMDKDFDLTNPHTFNAASEYMNVVGSNASDENVANRQLQDKLTHHLDTVEVHLLKEISRRSSSFFSALSNLQSLHNETEECVGKIQDLRKKLASVSARTAENGLEVVRLKHRRGNVGMLYGAVKLLVDIKHSQPTIQALLGQADYVGVLDLIE 351          

BLAST of EWM27149.1 vs. NCBI_GenBank
Match: gi|1032650112|gb|OAQ31396.1| (Vps54-domain-containing protein [Mortierella elongata AG-77])

HSP 1 Score: 207.223 bits (526), Expect = 4.917e-51
Identity = 192/728 (26.37%), Postives = 325/728 (44.64%), Query Frame = 0

Query:  202 QQPELDEALKAQLVPRVRGLVRLCRLSKALERYRGRLTASLKEVVKTVLTEYLGVAEYQRGEDKVGADGAVNTPAEAASPPLPVNGTSGLPTAAAAATSTEENAGLLGKGVRAMKAESFLGCLKLCFEQMLLPMTGVGLIHTFLEEQISE-ELSRILDRRPVPSSSLHSTHQSQGELST------------------GSPGETGGEGSFGHVNREPSLPPSSSPAVSNSSRTDAGSVPPSPTPSPSNKFKTFLEKRRLETGGGASKSGTSTVGSLGIGGKQQTQAPFKDEDQIKVLELQACKKL-------NDEILKTLCDLCYRHIVSLLVARREANVSMDLAEIKVLWDSTMSFLGAVEQISGTAGFGLKSTLLTQAKQFVEHVHKGGVGRLLAALDAERWVQTDVGAERQAEIDHLMAGRAVLAARASSSVQSLQEDRL-LSFPKEDSISDVQDLSHSRESGELSISSSISNDGHSENRHRFVATEVI-IGEERYKVVYSAVLLISLLNSYLNVAANFPMLAMDVMQCVVNLLRLFNTRTAQLVLGAGAIQATARLRSITAKHLALASQCLGLILVLLPHVRAALAAHLQTKQQAFLIELDRLRQEYAEHQERVLTKFVAIMGELIA-HSAANTGLRETDWDAL----------GSAACRFVEEVIKGTTTMHRVLYQILPPTQVQDIFNRVFDLLGHRLKEYLDHARPTTNAGRERLVDEVSHL 890
            +Q E +  L+ +++P V+GL+R  R   AL+ +R ++   LK ++K+         +Y                     PP P    S +P +A      +E    L   +R M   +F   L   +  +L  +  V L H  +   I + E     D     +SS   + Q    +                    GS GE  G  S G      +  P ++P  +   R    +   S   SP   F            GG      +  G+L I            ED+   + + A  +L       ++EIL  + DL +     L+  R E +  +  AE+  L   T SF+   E +SG   +GL++ +L+Q+K F+   H     +L  A+  ++W Q +V    Q+  + + A  ++    A +   ++  + L LS P    + D      SR   + S  +  S  G S++     +T V+ I  E + VV  +++L+ +L  YL++  N   L  DVMQ ++ LL+LFN+RT Q++LGAGA+++ A L++ITAKHLALASQ L +   L+P+V+  + + +  KQ   L E DR+ +++ EHQ  + +K VAIM E +A H  + T     D+DAL          G++   F++ ++K  TT+H+VL + LP T ++ + + V  +   RL+E +      T  G+ RL  +V++ 
Sbjct:  552 KQLERESDLQEKMMPLVQGLLRANRAGVALQTFREQILQELKNIIKS---------QY--------------------PPPDPDVQNSDVPLSAKK----KEQMAALTNQLRTMDPVTFYKMLSFIYAFLLGALQRVSLYHQLMLHCIRKAEAQGTGDLMMADTSSDSDSMQESASIREMKEGEHEGEEYEELDDQEGSDGENAG-FSLG------TSTPGATPLQATIGRKRMTAQSGSKVGSPRTPF-----------SGGPMSPPVNRFGALQI------------EDEDGQMGINAVAQLFRQLETESEEILFAVADLAHSRCAKLMGMRSEQSAKLLPAEVYKLLKLTWSFVLQSELLSGYMCYGLRTAILSQSKAFLIQFHMEKTSQLALAVGEDQWAQVEVPWSSQSTANEITAISSLSIQEARNKDFTMVFNVLSLSAPPIQGMDDHDSFIQSRLGSKGSNGALNSGKGDSKSTEIPASTRVLSIRGEVFFVVGCSLVLLDILAEYLDILNNITALTTDVMQRIIELLKLFNSRTCQVILGAGAMRS-AGLKNITAKHLALASQSLSVFATLIPYVKDWIRSKMTDKQMVMLTEFDRILRDFQEHQNEIHSKLVAIMTERLALHIRSMTNPAVVDYDALNKPTQQLANGGASVNAFMDGLVKDATTLHKVLSRFLPATSMRRVIDEVLQIFTTRLEEEIQKLPIATPLGKTRLQADVAYF 1215          

HSP 2 Score: 101.293 bits (251), Expect = 4.938e-18
Identity = 67/219 (30.59%), Postives = 104/219 (47.49%), Query Frame = 0

Query:   43 VPAIFFHRDFQLSEPALFDRVVIRATPDT--------------QERLSQYLDVVETCLLKQISSRSQHFFEALITLQDVRDRLAQACGQVLRLRSGLHDIDQKTVRGMIRIPRLAQRKVNLAALLEKLRLLQDVQRSTTLVQSLLGAGDFLGALDVLEDGQGLSEHSCVRLPLQQPELDEALKAQLVPR------------VRGLVRLCRLSKALERYR 235
            VP+IFF  +F    P  FD V  +  P                QE+LS YLD VE  L+ +IS RS  FFEAL  LQ +  +  +   Q+  LR+ L  ID    +  + + RL +R+ NL  L   +R++++++ +  ++Q LLG  D+ GALD+++  Q +     ++     P  D +L   LVP             +RG++ L   S  L   R
Sbjct:  276 VPSIFFDPNFNFENPRTFDLVCEQTGPAETKNSTTSIVTNSILQEKLSHYLDTVEIHLMTEISRRSTSFFEALSNLQALHSQTLECVSQIHVLRAQLARIDHTQSKQGLEVVRLKRRRRNLGLLHNGIRMVKEIRSTQPMIQVLLGKADYFGALDLIDGAQQVLIEGDMKYLEATPGFDPSLT--LVPSSSAIMPSPRSLDIRGVLALVHFSGQLTEMR 492