EWM27320.1, cds3351 (CDS) Nannochloropsis gaditana

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|585109520|gb|EWM27320.1| (trna (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Nannochloropsis gaditana])

HSP 1 Score: 1557.35 bits (4031), Expect = 0.000e+0
Identity = 764/764 (100.00%), Postives = 764/764 (100.00%), Query Frame = 0

Query:    1 MTLLPPHSHTFIHTQIHYQQRWYPATLWWSVGYRGVPSSLSSTLKCISCDICWRATQPKRQKPYHRLEAWQELGVASMRFSLLTLLLLLHQSRGFFWLPAAPGLLLERFRGRSPFRVPLRVAASSRGLPEDDPTSPLPILPPGLENFRRLLSSLPTDDESKAQSLYLISLGDQYAAATARSIASRGGRRVPGCVSVVHMTVRFSSDGSAVHVDGISDARLSYGLVALVAQGLTGESPETILSLEPTTLPKALGLTGGMMQQAPGRHNGLANIVQTVQDEVLARLHESRVPDFQGERGAREAILAAPAERPMLTTAGTKEFAVPVAVDPSHAAPTPAISFPTPAQLPLLTHSFPSEEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLNQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVMGRRYPKVATGHYARAVPAAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDSLAPGQYAALYQDVGEEGSECLGAGVISEETWRIPQAPARLTVNRR 764
            MTLLPPHSHTFIHTQIHYQQRWYPATLWWSVGYRGVPSSLSSTLKCISCDICWRATQPKRQKPYHRLEAWQELGVASMRFSLLTLLLLLHQSRGFFWLPAAPGLLLERFRGRSPFRVPLRVAASSRGLPEDDPTSPLPILPPGLENFRRLLSSLPTDDESKAQSLYLISLGDQYAAATARSIASRGGRRVPGCVSVVHMTVRFSSDGSAVHVDGISDARLSYGLVALVAQGLTGESPETILSLEPTTLPKALGLTGGMMQQAPGRHNGLANIVQTVQDEVLARLHESRVPDFQGERGAREAILAAPAERPMLTTAGTKEFAVPVAVDPSHAAPTPAISFPTPAQLPLLTHSFPSEEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLNQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVMGRRYPKVATGHYARAVPAAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDSLAPGQYAALYQDVGEEGSECLGAGVISEETWRIPQAPARLTVNRR
Sbjct:    1 MTLLPPHSHTFIHTQIHYQQRWYPATLWWSVGYRGVPSSLSSTLKCISCDICWRATQPKRQKPYHRLEAWQELGVASMRFSLLTLLLLLHQSRGFFWLPAAPGLLLERFRGRSPFRVPLRVAASSRGLPEDDPTSPLPILPPGLENFRRLLSSLPTDDESKAQSLYLISLGDQYAAATARSIASRGGRRVPGCVSVVHMTVRFSSDGSAVHVDGISDARLSYGLVALVAQGLTGESPETILSLEPTTLPKALGLTGGMMQQAPGRHNGLANIVQTVQDEVLARLHESRVPDFQGERGAREAILAAPAERPMLTTAGTKEFAVPVAVDPSHAAPTPAISFPTPAQLPLLTHSFPSEEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLNQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVMGRRYPKVATGHYARAVPAAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDSLAPGQYAALYQDVGEEGSECLGAGVISEETWRIPQAPARLTVNRR 764          

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|873229370|emb|CEM13165.1| (unnamed protein product [Vitrella brassicaformis CCMP3155])

HSP 1 Score: 429.098 bits (1102), Expect = 6.751e-138
Identity = 263/624 (42.15%), Postives = 350/624 (56.09%), Query Frame = 0

Query:  134 TSPLPILPPGLENFRRLLSSLPTDDESKAQSLYLISLGDQYAAATARSIASRGG-----RRVPGCVSVVHMTVRFSSDGSAVHVDGISDARLSYGLVALVAQGLTGESPETILSLEPTTLPKALGLTGGMMQQAPGRHNGLANIVQTVQDEVLARLHESRVPDFQGERGAREAILAAPAERPMLTTAGTKEFAVPVAVDPSHAAPTPAISFPTPAQLPLLTHSFPSEEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLNQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVMGRRYPKVATGHYARAVPAAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDS-LAPGQYAALYQDVGEEGSECLGAGVISEETWR 751
            TS  P LP   E     L SL      K + L+L+ LGD+      +      G      +VPGCV+ V +      D  AV V G +DAR+S GLVALVA+ L G     +L++ P     +  +   +    PGR NGL +++ T+Q ++ +R+                A +    E     +AG++                       P   P    S  + EVAVLLSGGVDSSVA  LL  +G +  AFYL+IWLEDEL+HL +CPWEED  FA   C Q   +PLE + LQ  YW  VV +TI EA  GRTPNPD++CN+ VKFG F D  GR + +VATGHYA+        +  G VRL RSPDRIKDQTYFL  L+QEQLA A FPIG ++K ++R +A    LPT +R+DSQGICFLGKLR++DFL HYLG++PG +V +    ++G+H GLW HT+GQRKG+ P L +     GP++V  KD   N L VTN  +++          +  R+  +V+ + W+ G PP  L   G ++M EVQ+RHGP +H  +RVR+L DG GE   S E+ L  +DS +APGQ+ A Y      G ECLG G I  ETW+
Sbjct:   28 TSKDPQLPLQAEG---ALESLRAQPSQKDRFLHLVRLGDELTRRNQQPGGLPDGFEQRAEKVPGCVATVKVLATLDDD--AVKVYGTADARVSQGLVALVAEALEGLPAPRVLAVPPDAFAGSTAMASFL---TPGRLNGLRSLIMTIQRQIASRV----------------AGVGEEQESETSASAGSEGL---------------------PLGPPSFVLSEAAGEVAVLLSGGVDSSVAARLLVDRGERVRAFYLRIWLEDELSHLGECPWEEDLRFARDTCDQL-KIPLELVSLQDSYWDSVVRYTISEAAAGRTPNPDVMCNTRVKFGAFCDDHGRHFKRVATGHYAQVRR----DQENGPVRLFRSPDRIKDQTYFLCGLSQEQLAMADFPIGTFDKARVRQLARDYSLPTASRRDSQGICFLGKLRWEDFLTHYLGKNPGPIVDVAGGELLGEHHGLWLHTVGQRKGLGPYLRDKMVHRGPFFVVGKDPDTNTLLVTNDYDAI----------DRPRQELQVESIGWIAGKPPASLSTAGDAIM-EVQLRHGPKTHM-ARVRLL-DGDGE---SAEVHLEEKDSGIAPGQFVAFYA-----GDECLGGGRI-RETWK 579          

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|299470175|emb|CBN78203.1| (tRNA-methyltransferase [Ectocarpus siliculosus])

HSP 1 Score: 407.527 bits (1046), Expect = 3.519e-131
Identity = 222/436 (50.92%), Postives = 279/436 (63.99%), Query Frame = 0

Query:  359 VLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLNQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVMGRRYPKVATGHYARAVPAAGGKEGE------------------------------GLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDD-------GRGEG-----------PSSLEITLPREDS-LAPGQYAALYQDVGEEGSECLGAGVI 745
            +LLSGGVDSSVA+ LL  QGYK  AFYLKIWLEDELAHLN+CPWEED ++A+AVC QAG VPLE +PLQ+EYW QVV++T++EA+EGRTPNPD++CNS +KFG+FYD +G+ +  VATGHYAR   + G  +                                G VRL+ SPD +KDQTYFL  LTQ+QL +A FP+G Y K QIR +AE  DLPTK RKDSQGICFLGKL+FD+FL+HYLG SPG +V  +    +G+H+GLWFHTIGQRKG+ P L      LGPWYVA KD+ RNVL VTN  E+++            R  FRV+ + W+ G PP E L    S+ L V+ RHGP   R   +R++ +       GR  G           P+   + L  +DS LAPGQ+AA Y      G EC+G+GV+
Sbjct:    1 MLLSGGVDSSVAMRLLLDQGYKIRAFYLKIWLEDELAHLNECPWEEDLAYATAVCEQAG-VPLEVVPLQREYWDQVVSYTVREAREGRTPNPDVMCNSRIKFGMFYDHVGKHFRHVATGHYARLEHSGGSDDANATPSDRSAAAAAAAAGTEEPPVRVAAGSDRGRVRLLLSPDAVKDQTYFLCALTQDQLRKALFPLGVYTKDQIRELAESYDLPTKHRKDSQGICFLGKLKFDEFLQHYLGTSPGAIV-DQTGRHIGEHKGLWFHTIGQRKGVGPGLVPGVVNLGPWYVADKDMERNVLVVTNDREAIA----------SPREVFRVEALQWISGRPPAE-LARDRSMRLRVKTRHGPQM-RDCLLRLVPEVAPGDSGGRAGGAEATVEDRVGSPTRGYVNLDSKDSALAPGQFAAFYL-----GGECVGSGVM 417          

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|551646166|ref|XP_005825870.1| (hypothetical protein GUITHDRAFT_76798 [Guillardia theta CCMP2712] >gi|428169961|gb|EKX38890.1| hypothetical protein GUITHDRAFT_76798 [Guillardia theta CCMP2712])

HSP 1 Score: 404.06 bits (1037), Expect = 1.294e-130
Identity = 209/397 (52.64%), Postives = 277/397 (69.77%), Query Frame = 0

Query:  354 SEEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLNQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVMGRRYPKVATGHYARAVPAAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDSLAPGQYAALYQDVGEEGSECLGAGVISEETW 750
            SEEVAVLLSGGVDSSVAL L+++QG+KP AFYL+IWLEDELAHL +CPWEEDWS+AS+V  Q   VPLE++ LQ+EYW QVV + I+EAK GRTPNPDI+CNS +KFG+F + +G+ + +VA+GHYA +     G       RLM SPD++KDQTYFL  L Q+QL  A FPIGGY K ++R +A   +LPT+ RKDSQGICFLGKL+F+DF+ H+LGE PG V+       +G+H+GLWF+TIGQRKG+  + T     LGPW+VA KD  RN+L+V+N  + +      EGP    RR+F V+ + W+ G+ P +      +L L ++ RHGPN H G  +  L  G+  G  SL+    R+  LAPGQ+AALY     +G EC+G G+I++ T+
Sbjct:   43 SEEVAVLLSGGVDSSVALELVRRQGFKPRAFYLRIWLEDELAHLGECPWEEDWSYASSVAEQL-KVPLESISLQEEYWEQVVEYLIREAKMGRTPNPDIMCNSRIKFGMFEEYVGKHFSRVASGHYAVSCHDDVGTRPS---RLMMSPDKVKDQTYFLCNLRQDQLKHALFPIGGYTKEKVRELAREFNLPTQRRKDSQGICFLGKLKFEDFVGHHLGEDPGPVIDFHTGRELGEHRGLWFYTIGQRKGL-GSATRKVTHLGPWFVAGKDRERNMLYVSNQYDVI------EGP----RRSFDVEQINWMAGSLPED-----DALRLRIKTRHGPNIHDG--LLTLSQGKLSGRVSLD---GRDSGLAPGQWAALY-----DGEECIGGGMIADSTF 409          

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|922855341|gb|KOO23799.1| (tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Chrysochromulina sp. CCMP291])

HSP 1 Score: 402.905 bits (1034), Expect = 7.513e-130
Identity = 215/408 (52.70%), Postives = 269/408 (65.93%), Query Frame = 0

Query:  355 EEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHL--NQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVMGRRYPKVATGHYARAVP-------------AAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDSLAPGQYAALYQDVGEEGSECLGAGVISE 747
            EEVAVLLSGGVDSSVALH L   G++  AFYL+IWLEDE AH     CPWEEDW++  AVC QAG VPLEA+ LQKEY   VV + + EA  GRTPNPDI+CNS +KFGVF+D +G  +  VA+GHYAR  P             +AGGK    +V L+RS D  KDQTYFLS+L QEQL+ A FPIGG  K ++RA A  L LP + RKDSQGICFLG+L +DDFLR +LGE+PG ++  E   +VG H+GLW+HT+GQR+G+ P L NA+R  GPW+V  KD   NVL+ T S        TG    ++AR  FR  G+ WV G PP   ++PG  L L+V+VRHG  +H  + V +  DG G   + +E+   R+  LAPGQ+AA Y     +G  CLG+GVI E
Sbjct:   67 EEVAVLLSGGVDSSVALHQLLAAGHRCRAFYLRIWLEDEQAHAARGDCPWEEDWAYCEAVCEQAG-VPLEAVSLQKEYQEHVVGYLVAEAAAGRTPNPDIMCNSRIKFGVFHDRVGCHFGHVASGHYARTAPTRSTGSRATVPFSSAGGKP---MVELLRSADAHKDQTYFLSQLRQEQLSSAIFPIGGMSKPEVRAAAHALHLPNRARKDSQGICFLGQLNYDDFLRDHLGEAPGHIIEFETGEIVGTHRGLWYHTVGQRRGLGPVLNNAFRAFGPWHVVHKDFEANVLYATRSY-------TG---ADKARNAFRTAGINWVAGHPP--TVLPGVPLHLQVKVRHGAQTHEAT-VALHADGYG---AEVELA-ERDKGLAPGQFAAFY-----DGEVCLGSGVIVE 448          

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|397601234|gb|EJK57851.1| (hypothetical protein THAOC_22066 [Thalassiosira oceanica])

HSP 1 Score: 404.445 bits (1038), Expect = 9.923e-128
Identity = 236/574 (41.11%), Postives = 327/574 (56.97%), Query Frame = 0

Query:  188 RRVPGCVSVVHMTVRFSSDGSAVHVDGISDARLSYGLVALVAQGLTGE--SPETILSLEPTTLPKALGLTGGMMQQAPGRHNGLANIVQTVQDEVLARLHESRVPDFQGERGAREAILAAPAERPMLTTAGTKEFAVPVAVDPSHAAPTPAISFPTPAQLPLLTHSFPSEEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLNQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVF---YDVMGRRYPKVATGHYAR--AVPAAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGAS---LMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPRED-SLAPGQYAALYQDVGEEGSECLGAGVISEETW 750
            +RVPGC++ V   VR +   + V + G SDA LS GL+A+VA  L+G   +   IL L+  +L  ALGL   +     GR++G+A++++ +QD++ + L +S     Q    +R+AI    +++P                                              VA+LLSGGVDSSVALHLL QQGY   A+YL+IWLEDELAHL +CPWE+D      VC  AGNVPLE + L KEY  +VV +TI+EA++GRTPNPDI+CNS VKFG F    D  G ++  VA+GHYAR    P           RL R+PD +KDQ+YFL  LTQ+QL++  FPIG Y+K ++R +A   +LP + R DSQG+CFLGK++FD+FL  YLG  PG+VV      V+GKH GLW+HT+GQRKGI   +       GPWYV AKD  ++V++V+N  +        E    +AR  F V+ + W+    P + +        +  ++++RHGP   +GS +++  DG     SS  + L  +D  LAPGQY   Y    +   E LGAGVISE+ W
Sbjct:  127 QRVPGCIATV--LVRATLSRNIVTISGKSDALLSGGLIAIVADVLSGVDVTAHEILQLDANSLTSALGLKPIL---NTGRNDGVASMIRVIQDQIRSLLGDSSGNTIQPS--SRDAIRNETSDKPT---------------------------------------------VAMLLSGGVDSSVALHLLLQQGYNVTAYYLRIWLEDELAHLGECPWEDDLHVCQEVCEHAGNVPLETVSLGKEYRDRVVQYTIEEAQQGRTPNPDIMCNSRVKFGCFLEYIDQSGMKFDHVASGHYARLEDEPRESSPGTTRAKRLFRAPDPVKDQSYFLCALTQKQLSKVLFPIGQYKKSEVRQLASEFNLPNRNRPDSQGLCFLGKVKFDEFLSSYLGSRPGDVVDAMNGEVIGKHNGLWYHTVGQRKGIGKVMFPLATAHGPWYVVAKDQSKDVVYVSNRYD--------EDDFAKARSEFEVEDVRWISNQHPAQAMSSDGKWEVVRFDMKIRHGPKIVQGS-LQLKGDG-----SSGNVKLDLKDGGLAPGQYVVFYSQTSD---EVLGAGVISEKHW 631          

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|1210511349|dbj|GAX29580.1| (tRNA-uridine 2-sulfurtransferase [Fistulifera solaris])

HSP 1 Score: 389.037 bits (998), Expect = 3.694e-123
Identity = 236/590 (40.00%), Postives = 322/590 (54.58%), Query Frame = 0

Query:  189 RVPGCVSVVHMTVRFSSDGSAVH-------VDGISDARLSYGLVALVAQGLTGESPETILSLEPTTLPKALGLTGGMMQQAPGRHNGLANIVQTVQDEVLARLHESRVPDFQGERGAREAILAAPAERPMLTTAGTKEFAVPVAVDPSHAAPTPAISFPTPAQLPLLTHSFPSEEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLN-QCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVM----GRRYPKVATGHYARAVPAAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDS-LAPGQYAALYQDVGEEGSECLGAGVISEETWRIP-----QAPARLT 760
            +VPGC + VH+ +  +SD S          + G +DA +S GLVA++A  +  +S +TI  +   ++   LGL   +   +PGR++G+A++ +         LH+S    F                 P  +T   K   +  +  PS                           VA+LLSGGVDSSVAL LL QQ Y+  AFYLKIWL+DE AHL   CPWEEDW     VC QA +VPL A+ LQ+EY  +VV + +++A  GRTPNPDILCNS +KFG FYDV+      ++  +ATGHYAR      G      V L R+PD IKDQ+YFL  LTQEQL +  FP+G  EK Q+R +A+  +LP + R DSQG+CFLG+++FD FL+ YLG  PG VV      V+G+HQG+WFHTIGQRKGI   L       GPWYV +KD  + +++ TN  E            +  R+   ++ +TW+ G  P  ++  G +  L +++RHGP   RG  +  L+D     PS   + L + DS LAPGQY   Y D G    ECLG+G++SE  W  P     QA  +LT
Sbjct:   41 KVPGCTANVHIQLVLTSDSSTTRNSRTIQSLQGTADALVSQGLVAVIASCVEQQSEDTIGQISAESITARLGLQQHL---SPGRNDGVASLWRI--------LHQSLQSSF-----------------PASSTTSAK--TIKYSQQPS---------------------------VALLLSGGVDSSVALRLLLQQNYRVTAFYLKIWLQDENAHLGGTCPWEEDWKICQQVCEQA-DVPLRAISLQQEYHERVVEYAMEQASLGRTPNPDILCNSRIKFGCFYDVLMSQQSEQFDYIATGHYARVERDDNGVS----VCLFRAPDAIKDQSYFLCTLTQEQLQKTLFPLGHLEKTQVRQLADEFELPNRHRPDSQGLCFLGQVKFDQFLKSYLGTQPGSVVDAVTGDVIGQHQGVWFHTIGQRKGIGKALNPQATARGPWYVVSKDPVKRIVYCTNQYELF----------DAQRKRVELEDVTWMSGQVPA-MVSDGNACRLTMKLRHGPRLARG--LLQLNDAE---PSKGTVELEQMDSGLAPGQYVVFYADDG----ECLGSGIMSESQWNRPICLETQAVEQLT 548          

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|1210528820|dbj|GAX12463.1| (tRNA-uridine 2-sulfurtransferase [Fistulifera solaris])

HSP 1 Score: 388.267 bits (996), Expect = 3.435e-122
Identity = 224/577 (38.82%), Postives = 319/577 (55.29%), Query Frame = 0

Query:  189 RVPGCVSVVHMTVRFSSDGSAV-------HVDGISDARLSYGLVALVAQGLTGESPETILSLEPTTLPKALGLTGGMMQQAPGRHNGLANIVQTVQDEVLARLHESRVPDFQGERGAREAILAAPAERPMLTTAGTKEFAVPVAVDPSHAAPTPAISFPTPAQLPLLTHSFPSEEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLN-QCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVM----GRRYPKVATGHYARAVPAAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDSLAPGQYAALYQDVGEEGSECLGAGVISEETWRIP 753
            +VPGC + VH+ +  +SD S          ++G +DA +S GLVA++A  +  ES + I  +   ++   LGL   +   +PGR++G+A++ +T+Q  + +                     ++PA             +   + +PS  +  P+                    VA+LLSGGVDSSVAL LL QQ Y+  AFYLKIWL+DE AHL   CPWEEDW     VC QA NVPL+A+ LQ+EY  +VV + +++A  GRTPNPDILCNS +KFG FYDV+      ++  +ATGHYAR        + +   RL R+PD +KDQ+YFL  LTQEQL +  FP+G  EK Q+R +A+  +LP + R DSQG+CFLG+++FD FL  YLG  PG VV       +G+HQG+WFHTIGQRKGI   L       GPWYV +KD  + +++ TN  E            +  R+   ++ +TW+ G  P  ++  G +  L +++RHGP   RG  +++ +    +G   LE     +  LAPGQY   Y D G    ECLG+G++SE  W  P
Sbjct:   88 KVPGCTANVHIQLILTSDASTTINSRTIQSLEGTADALVSQGLVAVIASCVEQESEDAIGQISAESITTRLGLQQHL---SPGRNDGVASLWRTLQQSLQS---------------------SSPA-------------SFITSTEPSTQSQQPS--------------------VALLLSGGVDSSVALRLLLQQNYRVTAFYLKIWLQDENAHLGGTCPWEEDWKICQQVCEQA-NVPLQAISLQQEYHERVVEYAMEQASLGRTPNPDILCNSRIKFGCFYDVLMSQQAEQFDYIATGHYARVER----DDDDASARLFRAPDAVKDQSYFLCTLTQEQLQKTLFPLGHLEKAQVRQLADEFELPNRHRPDSQGLCFLGQVKFDQFLESYLGTQPGSVVDAVTGDNIGQHQGVWFHTIGQRKGIGKALNPQATARGPWYVVSKDPAQRIVYCTNQYELF----------DAQRKHVELEDITWMSGQLPA-MVSGGNACHLTMKLRHGPRLARG-LLQLNNADFSKGTVELE---QMDSGLAPGQYVVFYADDG----ECLGSGIMSESQWNRP 583          

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|219126278|ref|XP_002183388.1| (predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1] >gi|217405144|gb|EEC45088.1| predicted protein, partial [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 368.622 bits (945), Expect = 9.529e-118
Identity = 198/393 (50.38%), Postives = 260/393 (66.16%), Query Frame = 0

Query:  357 VAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLNQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVMGRR-YPKVATGHYARAVPAAGGKEGE-GLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRTFRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDSLAPGQYAALYQDVGEEGSECLGAGVISE 747
            VA+LLSGGVDSSVAL+LL QQGY   AFYLKIWLEDELAHL  CPWE+D+     VC QAG +PLE + LQ  Y ++V+A+T+ EA++GRTPNPDI+CNS VKFG FY+ + +R +  VA+GHYA+ V     K+ E G +RL+R+PD IKDQ+YFL  LTQ+QL R  FPIG  +K Q+R +A+   LP + R DSQG+CFLGK++FD+FL  YLG  PG +V      VVG+H G+W+HT+GQRKGI   L       GPWYV AKD G++V++ +N  +        E     +R  F V+ + W+ G  PP LL  G      +++RHGP+  +G+ +R L +  GEG   L+    ++  LAPGQY   Y+D      ECLGAGVISE
Sbjct:    1 VALLLSGGVDSSVALNLLVQQGYDVTAFYLKIWLEDELAHLGHCPWEDDYRVCQEVCEQAG-IPLETVSLQNAYKSRVIAYTVHEAEQGRTPNPDIMCNSRVKFGCFYEAIDQREFDFVASGHYAQLV-----KDTETGSMRLLRAPDPIKDQSYFLCTLTQDQLQRVLFPIGHLQKSQVRDLADEFILPNRLRPDSQGLCFLGKVKFDEFLAVYLGTRPGPIVDAGTGDVVGQHHGVWYHTVGQRKGIGRVLDPLATSRGPWYVVAKDPGKDVVYCSNQYD--------EDIFTASRSEFAVENIHWIAGQAPPNLL-KGDGGRFVMKIRHGPDLVKGT-LR-LTNTAGEGDVKLD---SKDGGLAPGQYVVFYKD-----EECLGAGVISE 368          

BLAST of EWM27320.1 vs. NCBI_GenBank
Match: gi|546315177|ref|XP_005715790.1| (unnamed protein product [Chondrus crispus] >gi|507112283|emb|CDF35971.1| unnamed protein product [Chondrus crispus])

HSP 1 Score: 374.4 bits (960), Expect = 1.709e-116
Identity = 201/411 (48.91%), Postives = 257/411 (62.53%), Query Frame = 0

Query:  355 EEVAVLLSGGVDSSVALHLLQQQGYKPHAFYLKIWLEDELAHLNQCPWEEDWSFASAVCRQAGNVPLEALPLQKEYWAQVVAHTIQEAKEGRTPNPDILCNSLVKFGVFYDVMGRRYPKVATGHYARAVPAAGGKEGEGLVRLMRSPDRIKDQTYFLSRLTQEQLARATFPIGGYEKHQIRAMAERLDLPTKTRKDSQGICFLGKLRFDDFLRHYLGESPGEVVWLEEDVVVGKHQGLWFHTIGQRKGIVPTLTNAYRPLGPWYVAAKDVGRNVLFVTNSPESLSEVGTGEGPGEEARRT-FRVDGMTWVRGAPPPELLVPGASLMLEVQVRHGPNSHRGSRVRILDDGRGEGPSSLEITLPREDS-LAPGQYAALYQDVGEEGSECLGAGVISEETWRIPQAPARLTVNR 763
            ++VAVLLSGGVDSSVALH +Q+ G +PHAFYLKIWLEDELAHL  CPWEED  +A+AVC+QA NV L  +P QK YW +VV++T+ EA+ GRTPNPD++CNS +KFG F++ +GR Y +VATGHYAR           GL  L  S D  KDQTYFL+ L QEQ+A ++FP+G   K+++R +A+  DLP KTRKDSQGICFLGKL+FDDFL H+LG   G +V  E    +G+H+G WF TIGQR+GI   L+      GPWYV  KDV RNV++V+    S           E+ +RT F  +   W+ G  P  L   GA   L V+ RHGP  H     R   +G        E+ L   D  LAPGQ++A Y D       CLG+ VI+ +   +  AP  +   R
Sbjct:  239 KDVAVLLSGGVDSSVALHQIQESGARPHAFYLKIWLEDELAHLGSCPWEEDMEYAAAVCQQA-NVKLTDVPFQKAYWDEVVSYTVSEARRGRTPNPDVMCNSRIKFGAFFNRIGRYYDRVATGHYARQTV----NPETGLAELRLSADMWKDQTYFLAHLRQEQIAASSFPLGNIPKNEVRELAKAYDLPNKTRKDSQGICFLGKLKFDDFLGHHLGREMGSLVEFETGRELGQHKGFWFFTIGQRRGI--GLSG-----GPWYVVCKDVERNVVYVSKEYHS-----------EKQQRTWFEFETPLWIAGEWPNALGFNGAQTALRVKTRHGPKFHNAVVTRTWAEGG-------EVELAEGDKGLAPGQFSAFYDD----EDRCLGSAVIANDV-HLADAPTDIIRRR 614          

HSP 2 Score: 67.3958 bits (163), Expect = 5.815e-8
Identity = 46/136 (33.82%), Postives = 72/136 (52.94%), Query Frame = 0

Query:  145 ENFRRLLSSLPTDDESKAQSLYLISLGDQYAAATARSIASRGGRRVPGCVSVVHMTVRFSSDGSAVHVDGISDARLSYGLVALVAQGLTGESPETILSLEPTTLPKALGLTGGMMQQAPGRHNGLANIVQTVQDEV 280
            ++ R + +SL T DE +A+ + LI L D   A +   +      RV GC S+ H+ V  S D   V + G SD+ ++ GL+A V  GL G + + + ++ P  L +A GL G      P R  GL +I+  VQ++V
Sbjct:   80 QHLRTIANSLGTLDEPQARYMRLIHLADSIPAMSDNELHP--STRVSGCTSISHVEVVMSDD-RTVKMRGFSDSMVARGLMAFVILGLDGCTADEVAAVMPLELLEASGLRG---SAGPSRMMGLTSILSYVQNKV 209